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An analytical mehod for the determination of sanguinarine and chelerythrine in feeds by liquid chromatography tandem mass spectrometry (LC-MS/MS) method was established in this experiment. Sanguinarine and chelerythrine were extracted twice with 15 mL, 0.2% hydrochloric acid acetonitrile solution in ultrasonic water bath for 20 min. The extract was purified by HLB, and filtered through micoron filter, and analyzed by LC-MS/MS. The method was validated. The results showed that the correlation coefficient was over 0.999 in the range of 1 to 100 ng/mL; the quantitation limit was 0.005 mg/kg; recoveries were ranged from 91.9% to 101%, and RSD was ranged from 2.00% to 7.96%. This method was accurate and suitable to determine sanguinarine and chelerythrine in feeds. [ABSTRACT FROM AUTHOR]

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