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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Serum-specific biomarkers between normal healthy individuals and populations with developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome) have not been fully defined. OBJECTIVE: To screen and identify the potential biomarkers of developmental cervical canal stenosis with Qi deficiency and blood stasis. METHODS: Serum samples were collected from nine patients with developmental cervical canal stenosis with Qi deficiency and blood stasis and eight healthy people. Differentially expressed proteins in serum were screened and identified using isotope relative labeling and absolute quantification combined with liquid chromatography tandem mass spectrometry. Western blot was used to verify some significant differentially expressed proteins. RESULTS AND CONCLUSION: A total of 61 differentially expressed proteins (P < 0.05) were identified using tandem mass spectrometry techniques. Compared with the healthy normal population group, 14 differentially expressed proteins such as complement component C1q receptor, apolipoprotein A4, and C-C motif chemokine ligand 18 were significantly upregulated, while 47 differentially expressed proteins such as myosin light chain 3, mitochondrial translation elongation factor, and nucleolar phosphoprotein 1 were significantly downregulated. The results of gene ontology enrichment analysis indicated that these differentially expressed proteins might participate in molecular functions such as regulation of chromosomal tissue, mitochondrial membrane tissue, and muscle system processes. Protein-protein interaction network analysis showed that 38 common differential proteins, including complement component C1q receptor, apolipoprotein A4, C-C motif chemokine ligand 18, myosin light chain 3, mitochondrial translation elongation factor, and nucleolar phosphoprotein 1, were located at functional network nodes between healthy normal individuals and those with developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome), and were closely related to the local energy metabolism of the cervical spine, the production of cervical vertebral osteocytes, and the formation of osteoclasts. The main differentially expressed protein myosin light chain 3 was validated using western blot assay, and the validation results were consistent with the proteomic results. To conclude, the preliminary discovery of differentially expressed proteins in serum between healthy normal individuals and those with developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome) through absolute quantitative technology combined with liquid chromatography tandem mass spectrometry technology suggests that myosin light chain 3 may be a specific serum marker for developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome). [ABSTRACT FROM AUTHOR]

BACKGROUND: Taking into account the stability of spinal fixation, the preferred approach for lateral lumbar interbody fusion is commonly the utilization of posterior bilateral pedicle screws, typically performed in two stages. An alternative method involving the posterior approach of unilateral fixation using lateral vertebral nail rods in the lateral decubitus position has shown potential for enhanced mechanical stability. This technique also offers additional advantages such as reduced operation time, lowered risks, and decreased costs, making it a promising area for further investigation. OBJECTIVE: To compare the biomechanical effect of lateral lumbar interbody fusion with four different types of posterior instruments. METHODS: A validated L3-5 finite element model was modified to simulate four different types of lateral lumbar interbody fusion: Model A: posterior bilateral pedicle screw fixation; Model B: posterior unilateral pedicle screw fixation; Model C: lateral bilateral screw fixation; Model D: lateral unilateral screw fixation. The stability in the range of physiological motion, and the stress difference of screw fixation and interbody fusion apparatus were compared among the models. RESULTS AND CONCLUSION: (1) When compared with the intact model, all reconstructive models displayed decreased motion range at L4-5. Model A had a more obvious range of motion decline. (2) In the flexion, lateral flexion and axial rotation, the differences between the peak stress of the lateral screw fixation and the peak stress of the posterior screw fixation were more than 67.74 MPa, 80.10 MPa and 43.95 MPa, respectively. (3) In terms of internal fixed stress distribution in different reconstructed models, the stress distribution of Model A and Model B screws was mainly concentrated in the body of the pedicle screw, while the peak stress of Model C and Model D screws was mainly concentrated in the tail of the screw. (4) It is suggested that Model A could obtain the best stability and reduce the risk of cage sinking and displacement. Model B could also provide better stability, and could be a choice for lateral fusion on the premise of ensuring stability and fixation. (5) Besides, Model C could achieve the same effect as Model D when the lateral road screw was selected. However, patients with lumbar instability should be cautious to choose unilateral lateral internal fixation, and excessive extension should be avoided to reduce the possibility of screw loosening and fixation fatigue. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: As a new and objective method for the measurement and treatment of muscle training, pressure biofeedback can provide real-time feedback to improve the accuracy and effectiveness of deep neck flexor exercises. However, there are few reports on clinical studies related to pressure biofeedback and neck deep muscle exercise in China. OBJECTIVE: To investigate the clinical efficacy of pressure biofeedback training combined with deep neck flexor exercises in patients with chronic neck pain. METHODS: A total of 60 patients with chronic neck pain treated in the Out-Patient Department and Orthopedic Ward of the First Affiliated Hospital of Guangxi University of Chinese Medicine from April to October 2021 were enrolled in this study. These patients were selected and divided into experimental group and control group. The former group used pressure biofeedback to perform deep neck flexor exercises, while the latter group performed regular deep neck flexor exercises. The primary outcome was the peak pressure measured by pressure biofeedback at each follow-up point (1, 2, and 3 months after treatment) and the secondary outcomes were peak pressure maintenance time as well as the Northwick Park Neck Pain Questionnaire score. RESULTS AND CONCLUSION: (1) There were significant differences in peak pressure value and peak pressure maintenance time before and after exercises in the two groups (P < 0.05). Compared with the control group, the peak pressure value and maintenance time were significantly higher in the experimental group at each time point (P < 0.05). (2) The Northwick Park Neck Pain Questionnaire score in each group was significantly lowered at each time point after exercises (P < 0.05), and the score in the experimental group was significantly lower than that in the control group at 2 and 3 months after exercises (P < 0.05). (3) To conclude, pressure biofeedback combined with deep neck flexor exercises can effectively alleviate the symptoms of chronic neck pain, feedback real-time exercise results for patients, and increase the sustainability of functional exercises, which is a simple, convenient, and low-cost treatment for neck pain and can be a part of the community re-education program for prophylaxis and treatment. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Practical Medicine / Shiyong Yixue Zazhi is the property of Journal of Practical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Hainan Medical University is the property of Journal of Hainan Medical College Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: Both Jack kyphoplasty and balloon kyphoplasty have satisfactory results in the treatment of osteoporotic vertebral compression fractures. However, whether the two methods are better or not is still controversial. Thus, this study systemically evaluated the efficacy and safety of Jack vertebral dilator kyphoplasty and balloon-kyphoplasty in the treatment of osteoporotic vertebral compression fracture. METHODS: Wanfang, VIP, CNKI, PubMed, EMBASE, the Cochrane Library, CBM and other databases were searched by computer before July 2020. The clinical controlled studies on Jack kyphoplasty and balloon kyphoplasty in the treatment of osteoporotic vertebral compression fracture were collected. After two evaluators independently selected literature, extracted data and evaluated the quality of methodology included in the study (according to Cochrane Handbook and NOS scale), meta-analysis was carried out by using Stata/SE 12.0 software. RESULTS: (1) Seven articles were included; the quality of the three randomized controlled trial articles was grade B, and the NOS scores of four cohort studies were greater than 5. A total of 473 patients were included in the final analysis, including 229 cases in Jack kyphoplasty group and 244 cases in balloon kyphoplasty group. (2) The results of meta-analysis showed that Jack kyphoplasty was better than balloon kyphoplasty in terms of Cobb angle (MD=-2.2, 95%CI:-4.07 to-0.36, P=0.001). Jack kyphoplasty was higher than balloon kyphoplasty in terms of anterior vertebral height (MD=2.02, 95%CI:1.08-2.96, P=0.000). Jack kyphoplasty was lower than balloon kyphoplasty in terms of cement leakage rate (RR=0.32, 95%CI:0.15-0.68, P=0.003). There were no significant differences in postoperative visual analogue scale score (MD=-0.07, 95%CI:-0.26-0.13, P > 0.05), bone cement injection (MD=-0.08, 95%CI:-0.21-0.06, P > 0.05], operation time (MD=-0.51, 95%CI:-4.85-3.83, P > 0.05], and intraoperative blood loss (MD=0.44, 95%CI:-0.47-1.35, P > 0.05) between the two groups. CONCLUSION: Compared with balloon kyphoplasty, Jack kyphoplasty has more advantages in reducing Cobb angle, improving anterior height of vertebral body and reducing cement leakage rate. However, this conclusion still needs to be further confirmed by large-sample and multi-center randomized controlled trials. [ABSTRACT FROM AUTHOR]

OBJECTIVE: It remains disputed whether three-dimensional (3D) model assisted screw placement technique and traditional screw placement technique in the treatment of spinal deformity. Thus, this study systematically assessed the difference between 3D model assisted screw placement technique and traditional screw placement technique in the treatment of spinal deformity. METHODS: A computer-based online search of CNKI, Wanfang, VIP, The Cochrane library, PubMed, CBM, and EMBASE was performed to retrieve clinical studies of comparison between 3D model assisted screw placement technique and traditional screw placement technique in the treatment of spinal deformity published before April 2020. Moreover, related papers were manually retrieved. After two evaluators independently selected literature, extracted data and evaluated the quality of methodology included in the study, meta-analysis was carried out by using Stata/SE 12.0 software. RESULTS: (1) Eight clinical controlled trials were included, and the quality of a randomized controlled trial was grade B. NOS scores of all cohort studies were greater than 5. A total of 479 patients were included in the final analysis, and divided into model group (n=219) and traditional group (n=260). (2) The metaanalysis results showed that there were significant differences in Cobb angle (MD=-4.69, 95%CI:-8.05 to -1.32, P < 0.05), accuracy of pedicle screw placement (RR=1.11, 95%CI:1.08-1.14, P < 0.05), average screw placement time (MD=-1.52, 95%CI:-1.75 to -1.29, P < 0.05), operation time (MD=-28.59, 95%CI:-30.67 to -26.52, P < 0.05), intraoperative fluoroscopy times (MD=-3.11, 95%CI:-4.70 to -1.53, P < 0.05), and intraoperative blood loss (MD=-177.79, 95%CI:-250.08 to -105.50, P < 0.05) between the two methods. There was no significant difference in rate of complications (RR=0.94, 95%CI:0.75-1.17, P=0.576] between the two methods. CONCLUSION: Compared with the traditional screw placement technology, 3D model assisted screw placement technique has the advantages of good deformity correction, accurate and rapid screw placement, and less intraoperative blood loss. Given the limited quality of included studies, a large number of high-quality multicenter randomized controlled trials are needed to provide more evidence. [ABSTRACT FROM AUTHOR]

BACKGROUND: Clinically, Shaoyao Gancao Decoction has a satisfactory effect on the early pain of lumbar disc herniation, but its mechanism is not clear. As a new subject, network pharmacology has the characteristics of systematization, integrity and multi-channel regulation of signal pathway, which is consistent with the main characteristics of the theoretical system of traditional Chinese medicine. OBJECTIVE: To screen out the main active components of Shaoyao Gancao Decoction by network pharmacology, construct the active compound-target network, and elucidate the mechanism by which Shaoyao Gancao Decoction treats the early pain of lumbar disc herniation. METHODS: The active components of Shaoyao Gancao Decoction were retrieved by Traditional Chinese Medicine Systems Pharmacology Database, and the target genes of active components were selected by AMDE parameters. The relevant target genes of lumbar disc herniation were retrieved from OMIM and Gene Cards database, and the target genes of active components were matched with Shaoyao Gancao Decoction to obtain the intersection targets. The drug-disease-target network map was constructed by using Cytoscape 3.7.2., and the protein-protein interaction network of intersection targets was obtained by STRING platform. Gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using R software. Finally, the main active compounds of the obtained drugs were molecularly docked with key core proteins in the protein-protein interaction network using AutoDock Vina. RESULTS AND CONCLUSION: A total of 87 active ingredients such as quercetin, anthocyanin, kaempferol and 47 potential targets such as interleukin-6, interleukin-1β and matrix metalloproteinase 9 were contained in Shaoyao Gancao Decoction. The GO function enrichment analysis showed that Shaoyao Gancao Decoction was mainly involved in the molecular composition of membrane raft, membrane region and collagen containing extracellular matrix, affected the molecular functions of cytokine receptor binding, endopeptidase activity, receptor ligand activity, and participated in biological processes such as response to molecule of bacterial origin, response to reactive oxygen species and cellular respond to oxidative stress. The KEGG pathway enrichment analysis indicated that Shaoyao Gancao Decoction mainly played the role of anti-oxidation, anti-inflammation and regulated immune responses through tumor necrosis factor signaling pathway, hypoxia-inducible factor-1 signaling pathway and interleukin-17 signaling pathway. Molecular docking results showed that uercetin, menthol and kaempferol, the core compounds of Shaoyao Gancao Decoction, could be closely combined with the core targets, providing the necessary conditions for further exerting the anti-inflammatory and analgesic effects of Shaoyao Gancao Decoction. Overall findings indicate that Shaoyao Gancao Decoction mainly plays the role of anti-oxidation and anti-inflammation as well as regulate immune response through the characteristics of multi-component, multi-target and multi-channel (for example, quercetin can act on the target of matrix metalloproteinase-9 and regulate the tumor necrosis factor signaling pathway), and preliminarily explains the mechanism of Shaoyao Gancao Decoction in the treatment of early pain of lumbar disc herniation. [ABSTRACT FROM AUTHOR]

OBJECTIVE: The advantages and disadvantages of nicardipine are still controversial now. Some studies have reported that nicardipine used in orthopedic perioperative period reduces blood loss, but does not increase the risk of blood clots. However, these studies are small-sample trials, in the meantime, the results are still controversial. This paper discussed the effect of nicardipine on perioperative blood loss and blood transfusion in orthopedic department by meta-analysis. METHODS: PubMed, Web of Science, ClinicalTrials, Cochrane library, CNKI, CBM, Wanfang and VIP databases were retrieved by computer for randomized controlled trials of nicardipine in reducing perioperative blood loss in orthopedics published from inception to February 15, 2020. The application of nicardipine in orthopedic perioperative bleeding was searched, and selected according to the inclusion criteria. The literature that could be extracted and analyzed by meta-analysis should be focused on (i.e., including the main outcome measure: intraoperative blood loss; or secondary outcome measures: incidence of adverse reactions, awake time, operation time, postoperative extubation time, postoperative hemoglobin concentration, intraoperative blood transfusion volume, and one or more items of hematocrit). After screening the literature, extracting data and assessing the risk of bias included in the study, the Stata12.0 software was used for meta-analysis. RESULTS: Nine randomized controlled trials included 521 orthopedic surgery cases, with 275 cases in the nicardipine group and 246 patients in the control group. Results of meta-analysis showed that compared with other control groups included in this meta-analysis, (1) nicardipine reduced perioperative blood loss (SMD=-1.430, 95%CI: -2.570 to -0.290, P < 0.05); (2) nicardipine reduced the incidence of adverse reactions (OR=0.360, 95%CI: 0.130-1.020, P < 0.05), possibly prolonged patients' awake time (SMD=0.936, 95%CI: 0.220-1.653, P < 0.001); (3) nicardipine may prolong the postoperative extubation time (SMD=1.889, 95%CI: 1.544-2.233, P < 0.001). (4) There was no significant effect on operation time, postoperative hemoglobin concentration, intraoperative blood transfusion volume and postoperative hematocrit (P > 0.05). CONCLUSION: Nicardipine controlled hypotension is an effective method to reduce perioperative blood loss in orthopedic department. Nicardipine is safe for controlled hypotension in orthopedic perioperative period. However, it may prolong the awake time and extubation time of some patients. Therefore, clinicians should be more cautious when using this method. However, the above conclusions still need to be further confirmed by higher quality, large sample and multicenter randomized controlled trials. [ABSTRACT FROM AUTHOR]

OBJECTIVE: It remains disputed whether there is a difference in efficacy and safety between bone filling bag vertebroplasty and percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fracture. This study systematically analyzed the efficacy and safety of bone filling bag vertebroplasty and percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fracture. METHODS: A computer-based online search of CNKI, Wanfang, VIP, EMBASE, PubMed, CBM, and The Cochrane library was performed to retrieve randomized controlled trial studies and cohort studies regarding bone filling bag vertebroplasty and percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fracture published before March 2020. Moreover, related papers were retrieved manually. After two evaluators independently selected literature, extracted data and evaluated the quality of methodology included in the study, meta-analysis was carried out by using Stata/SE 12.0 software. RESULTS: (1) A total of seven articles were included, with 709 patients, of whom 321 were treated with bone filling bag vertebroplasty and 388 with percutaneous vertebroplasty. The quality of the two randomized controlled trials was grade B. The scores of NOS in two cohort studies were 8. The other three cohorts scored 7 on the NOS scale. (2) The meta-analysis results showed that there were significant differences between the two methods in reducing Cobb angle (MD=-1.92, 95%CI:-2.15 to -1.68, P < 0.05) and the leakage rate of bone cement (RR=0.15, 95%CI:0.07-0.30, P < 0.05). There was no significant difference between the two methods in reducing visual analogue scale score (MD=-0.03, 95%CI:-0.12-0.06, P=0.521) and Oswestry disability index (MD=0.16, 95%CI:-0.93-1.26, P=0.770). CONCLUSION: Compared with the conventional percutaneous vertebroplasty, bone filling bag vertebroplasty has a significant advantage in improving Cobb angle and reducing the leakage rate of bone cement. The clinical therapeutic effects of the two methods are similar in visual analogue scale score and Oswestry disability index. In view of the limited quality of the included literature, more high-quality multicenter randomized controlled trials are needed to provide evidence support for the above conclusion. [ABSTRACT FROM AUTHOR]

OBJECTIVE: It remains disputed whether percutaneous curved vertebroplasty and unilateral pedicle approach percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fracture. This study systematically analyzed the efficacy and safety of percutaneous curved vertebroplasty and unilateral pedicle approach percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fracture. METHODS: A computer-based online search of Wanfang, VIP, CNKI, PubMed, EMBASE, The Cochrane library, and CBM was performed to retrieve randomized controlled trial studies regarding percutaneous curved vertebroplasty and unilateral pedicle approach percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fracture published before January 2020. Related papers were manually searched. After two evaluators independently selected literature, data were extracted and the quality of methodology included in the study was evaluated. Meta-analysis was carried out by using Stata/SE 12.0 software. RESULTS: (1) A total of eight randomized controlled trials were conducted, including 628 patients, of whom 394 were treated with percutaneous curved vertebroplasty and 404 with unilateral pedicle approach percutaneous vertebroplasty. (2) The meta-analysis results showed that visual analogue scale score [MD=-0.20, 95%CI(-0.28, -0.12), P < 0.05], the leakage rate of bone cement [RR=0.30, 95%CI(0.15, 0.58), P < 0.05], and the total distribution rate of bone cement in the center of vertebral body [RR=1.27, 95%CI(1.12, 1.45), P < 0.05] were better in the percutaneous curved vertebroplasty group than those in the unilateral pedicle approach percutaneous vertebroplasty group. (3) There was no significant difference in Oswestry disability index score [MD=-4.83, 95%CI (-9.71, 0.05), P=0.052], operation time [MD=-1.45, 95%CI(-5.91, 2.82), P=0.488]and X-ray exposure times [MD=-0.70, 95%CI(-2.11, 0.71), P=0.33] between the two methods. CONCLUSION: Percutaneous curved vertebroplasty exhibits significant advantages in analgesic effect, low leakage rate of bone cement and the high total distribution rate of bone cement in the center over unilateral pedicle approach percutaneous vertebroplasty. Therefore, a large number of high-quality multicenter randomized controlled trials are needed to provide more evidence. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To evaluate the efficacy and safety of bisphosphonates for the treatment of osteoporosis patients with lumbar spinal fusion surgery using a meta-analysis system. Methods Publications from inception to January 2017 concerning bisphosphonates for the treatment of osteoporosis patients with lumbar spinal fusion surgery were retrieved through PubMed, ScienceDirect, Google Scholar, CNKI, VIP and Wanfang resource databases. Data analysis was performed using Stata 11.0 and the literatures that were finally included were analyzed systematically. Results Nine randomized controlled trials were included. The results of meta-analysis showed that there were significant differences between the bisphosphonates group and control group in the treatment of osteoporosis patients with lumbar spinal fusion surgery (P<0.01) for bone fusion (RR=1.138, 95% CI 1.064, 1.217), bone mineral density T-score (MD=0.038, 95% CI 0.025, 0.052), Oswestry Disability Index (MD=-4.36, 95% CI -6.372, -2.348), bone resorption biochemical marker CTX (MD=-0.401, 95% CI -0.511, -0.291) and visual analog scale (MD=0.940, 95% CI 0.548, 1.332). There were no significant differences in bone formation biochemical marker PINP (MD=-4.328, 95% CI -8.954, 0.298) and adverse reactions (RR=0.725，95% CI 0.420, 1.251) (P=0.067 and P=0.248). Conclusion There was no association between bisphosphonates treatment and nonunion of lumbar spinal bone, and safety is similar to that of the control group. In addition, it can effectively inhibit bone absorption, improve patients' bone mass and degree of postoperative functional recovery. [ABSTRACT FROM AUTHOR]