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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: At present, there is a lack of effective strategies that deal with annulus fibrosus defects in a majority of cases undergoing lumbar discectomy, which is one of the important causes of postoperative relapse of lumbar disc herniation. OBJECTIVE: To explore the effects of direct suturing and medical fibrin bonder for annulus fibrosus defects in goats undergoing resection of intervertebral discs. METHODS: L1/2-L6/7 samples (36 lumbar vertebrae) from six goats aged 6 months were randomized into three groups (n=12 per group): direct suture group, bonding group and control group (with no intervention). An annular incision of 4 mm in length was made on the disc using a circle cutter. A small amount of nucleus pulposus (about 0.1 g) was taken from each sample followed by direct suturing, bonding and no treatment in corresponding groups. X-ray and MRI observation of the lumbar vertebra was performed preoperatively and 12 weeks postoperatively. Height index of the lumbar intervertebral disc (DHI) was calculated to evaluate the lumbar degeneration. The goats were sacrificed at 12 weeks postoperatively and the defect sites were observed using light microscope and transmission electron microscope. RESULTS AND CONCLUSION: The DHI values in three groups at 12 weeks postoperatively were ranked as follows: direct suturing group > bonding group > control group. Lumbar T2WI MRI modified Pfirrmann grade was ranked as follows: direct suturing group < bonding group < control group. Histological Masuda degeneration grade was ranked as follows: direct suturing group < bonding group < control group. Performance of cell ultrastructure at repair site was ranked as follows: direct suturing group > bonding group > control group. To conclude, both direct suturing and fibrin bonder can effectively repair annulus fibrosus defects, and moreover, direct suturing is superior to bonding in the repair effect. [ABSTRACT FROM AUTHOR]