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Copyright of Biotechnology Bulletin is the property of Biotechnology Bulletin Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】The present paper aimed to study whether PpCASLUU3 was involved in the hypersensitive reaction in Physcomitrella patens after infection with B.cinerea. Knockout vector PpCASLUU3-PTN182 was constructed, which could provide theoretical basis for plant defense of landing plant. 【Method】In the principle, genome DNA extracted from P. patens was used as a template. With primers designed using Primer Premier 5, the homologous upstream and downstream fragments were amplified, and cloned into pMD-19 T. After being sequenced, the upstream and downstream genes were obtained with restriction enzyme EcoR I and EcoR V, BamH I and Sma I, respectively. Meanwhile PTN182 was digested with the same enzyme. Used T4-DNA ligase, the recombinant vector was transferred into E.coli DH5α. Positive clones were screened out, and the downstream arm was connected to PpCASLUU3.1-PTN182 vector by the same method.【Result】Complete PpCASLUU3.1-PTN182-ppcasluu3.2 knockout vector was obtained by PCR detection and enzyme digestion verification. After sequencing analysis, the similarity of inserted sequences PpCASLUU3.1 and PpCASLUU3.2 were 100 % and 99.63 % respectively.【Conclusion】It indicated that the knockout vector PpCASLUU3-PTN182 was successfully constructed. [ABSTRACT FROM AUTHOR]

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)