Objective • To study the effect of glucose on mouse CD4* T cell dififerentiatioiu Methods. Mouse naive CD4+ T cells cultured in the regulatory T cell (Treg)，Thl, Thl7 or Th2 differention condition were treated with different concentrations of glucose for 5 days. Treg, Thl, Thl7 or Th2 percentages were measured by flow cytometry. Quantitative real-time PCR was used to detect the gene expressions of related cytokines and transcriptional &ctors. Results * The proportions of Treg and Th2 as well as the gene expressions of Iransfonning growth factor-p, interleukin-4 (IL-4) and BL-13, and transcriptional factors，Foxp3 (forichead box P3) and Gata3 (GATA binding protein 3), were increased significantly with the treatment of increasing concentration of glucose. On the contrary, with the glucose treatment, the percentages of Thl and Ihl7 were reduced, and the gene expressions of the related cytokines and cytokine receptors, such as interferon-7, IL-17A, IL-17F, IL-22 and IL-23R, and the related transcriptional factors, Tbx21 (T-box transcription &ctor 21) and RORC (RAR related orphan receptor C)，were decreased consistently. Condu曲＞n. Glucose promotes Treg and Th2 differentiation whole inhibits Thl and Thl7 differentiation in vitro. [ABSTRACT FROM AUTHOR]