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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Serelaxin is a recombinant form of human relaxin 2. As endogenous anti-fibrosis active substances, serelaxin may relieve the symptoms and improve the prognosis of patients with acute heart failure. However, there is no report on the effect of serelaxin on hypoxia/reoxygenation injury of myocardial microvascular endothelial cells (H5V) in mice through regulating oxidative stress. OBJECTIVE: To investigate the role on serelaxin in a model of hypoxia/reoxygenation-induced mouse cardiac microvascular endothelial cells (H5V)injury. METHODS: To establish a mouse H5V hypoxia/reoxygenation injury model, cells were incubated under hypoxia for 3, 6, and 12 hours followed by 3 hours of reoxygenation. The optimal time of cellular hypoxia/reoxygenation was determined by cell viability and lactate dehydrogenase activity assay. Different concentrations of serelaxin (0, 60, 100, 140, 180 ng/mL) were selected to act in the H5V cellular hypoxia/reoxygenation injury model, and the optimal drug concentration for drug treatment of cellular hypoxia-reoxygenation injury was determined by the cell viability and lactate dehydrogenase activity assays. H5V cells were then divided into three groups: control, model (hypoxia/reoxygenation), and serelaxin (hypoxia/reoxygenation+180 ng/mL serelaxin) groups. Malondialdehyde, superoxide dismutase and reactive oxygen species levels were detected in each group. RESULTS AND CONCLUSION: Compared with the control group, cell proliferation was significantly reduced after hypoxia 6 hours/reoxygenation 3 hours and hypoxia 12 hours/reoxygenation 3 hours (P < 0.05), while the lactate dehydrogenase activity in cell supernatant was significantly increased (P < 0.05). Therefore, cells cultured under hypoxia 6 hours/reoxygenation 3 hours were selected for subsequent experiments. Cell viability was significantly increased in the serelaxin group compared with the model group (P < 0.05), while the lactate dehydrogenase activity in cell supernatant was significantly decreased (P < 0.05). Therefore, 180 ng/mL serelaxin was selected for subsequent experiments. Compared with the model group, the serelaxin group showed a significant decrease in reactive oxygen species and malondialdehyde levels but a significant increase in the level of superoxide dismutase (all P < 0.05). To conclude, H5V cells subjected to hypoxia 6 hours/reoxygenation 3 hours could establish a model of H5V vascular endothelial cell injury, and 180ng/mL serelaxin could improve the cell viability and anti-oxidative stress ability. [ABSTRACT FROM AUTHOR]

To explore the predictive value of rapid sequential organ failure score(qSOFA)combined with serum homocysteine( Hcy) and triacylglycerol(TG) in the short-term prognosis of acute pancreatitis(AP). The 210 AP patients who were admitted to Department of Gastroenterology, the First Affiliated Hospital of Suzhou University from September 2019 to September 2021 were selected as the research objects. According to the Atlanta AP classification guidelines,they were divided into 125 cases of mild AP (MAP group),45 cases of moderate and severe AP(MSAP group)and 40 cases of severe AP(SAP group). The qSOFA score, Hcy and TG levels of the three groups were compared. According to the prognosis 28 days after admission, all patients were divided into 192 cases in the survival group and 18 cases in the death group. Univariate and multivariate logistic regression were used to analyze the prognostic factors of AP patients. The predictive value of qSOFA score, Hcy and TG on the short-term prognosis of AP patients was analyzed by receiver operating characteristic (ROC) curve. The qSOFA score, Hcy and TG levels in SAP group and MSAP group were higher than those in MAP group, and those in SAP group were higher than those in MSAP group (P<0.05). Univariate and multivariate logistic regression analysis showed that higher qSOFA score and higher levels of TG and Hcy were the risk factors of death in AP patients(P<0. 05). The area under the curve (AUC) of qsofa score combined with Hcy and TG to predict the short-term prognosis of AP patients was 0.982, which was significantly higher than 0.715, 0.780 and 0.782 detected by the three indexes alone. qSOFA score, TG and Hcy levels are the influencing factors of short-term prognosis of AP patients, and the combined detection of qsofa score, Hcy and TG levels has high predictive value for the short-term prognosis of AP patients. [ABSTRACT FROM AUTHOR]