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Using microcrystalline cellulose (MC:C) and chitosan (CS) as raw materials, at first, oxidized microcrystalline cellulose (MCC (0)) was obtained by oxidation of microcrystalline cellulose. Then oxidized microcrystalline cellulose-chitosan graft (CS- g-MCC (0)) was prepared by 1 -ethyl- (3-T)imethylaminopropyl) carbodiimide hydrochloride (ET)C)/N-hydroxysuccinimide (NHS) couple crosslinking agent. The graft copolymer was characterized by Fourier transform-infrared spectrometer, contact angle measuring instrument, differential scanning calorimeter, and XRT) diffractometer, to determine whether the grafting was successful. The graftingrate was measured to investigate changes in water-based, thermal stability，and crystallinity. The results show that the performance of oxidized microcrystalline cellulose and chitosan as monomers was improved by grafting. When the molecular weight of CS was 50，000，the content of oxidized microcrystalline cellulose and chitosan was 1 5 2, the content ratio of EDC to NHS was 3 5 1，and the reaction temperature was 60 °C，the grafting rate reached a maximum of 61.2%. Through graft modification, CS and oxidized microcrystalline cellulose couldimprove and promote each other, so as to obtain more excellent biomedical materials. [ABSTRACT FROM AUTHOR]