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目的本研究旨在探讨抑制miR-4321在改善脓毒症相关急性肾损伤（Sepsis associated acute kidney injury， SA-AKI）中的作用和机制。方法采用RT-qPCR方法检测LPS诱导的HK2细胞模型和CLP模型小鼠肾脏组织中miR-3165、miR-4270和miR-4321的表达水平。利用miR-4321-Inhibitor抑制LPS诱导的HK2细胞损伤模型中miR-4321的表达，分别通过CCK-8分析、EdU染色分析和western blotting评估miR-4321对细胞增殖、细胞活力、细胞因子和凋亡相关蛋白水平的影响。利用miR-4321-Antago干预CLP模型小鼠肾组织中miR-4321的表达，通过H&E染色检测肾组织结构变化，比色法检测血清中肌酐和血尿氮水平，ELISA检测血清中白细胞介素6（IL-6）、白细胞介素1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的表达，IHC染色检测组织中mTOR水平，western blotting检测肾组织中凋亡相关蛋白表达水平。机制上，使用Targetscan软件预测miR-4321的靶基因，并通过双荧光素酶报告基因实验对靶基因进行验证。结果与对照组相比，LPS诱导的HK2细胞中miR-4321（n=3， t=7.154， P=0.001 3）表达升高；miR-4321-Inhibitor可促进HK2细胞增殖和细胞活力，并降低LPS诱导的IL-1β，IL-6和TNF-α水平的表达。体内实验证明miR-4321-Antago能够抑制CLP小鼠血清中肌酐和血尿氮水平，改善肾组织损伤，降低血清中IL-1β，IL-6和TNF-α水平，促进肾组织mTOR表达，抑制凋亡相关蛋白的表达。此外，mTOR是miR-4321的靶基因之一。结论抑制miR-4321可明显减轻脓毒症相关急性肾损伤，这一作用可能是通过增加mTOR的表达实现的。

Copyright of Journal of Xinyang Normal University Natural Science Edition is the property of Journal of Xinyang Normal University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Xinyang Normal University Natural Science Edition is the property of Journal of Xinyang Normal University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Ecology & Rural Environment is the property of Journal of Ecology & Rural Environment Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Geomatics & Information Science of Wuhan University is the property of Geomatics & Information Science of Wuhan University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Antibacterial and mould-proof polyurethane coatings (PU-PHMG) were prepared via chemically bonding polyurethane (PU) with polyhexamethylene guanidine (PHMG). The molecular structure and performances of the products were characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Abbe Refractometer and Atomic Force Microscope (AFM), etc. Properties of PU coatings affected by PHMG mass fraction was investigated. Results showed that when the mass fraction of PHMG was 1.01%, the bonding efficiency reached 93.59%, the inhibition rate against E.coli and S. aureus was above 99.5%. Then, the durabily antibacterial property of PU-PHMG was proved. Moreover, the antibacterial coating exhibited excellent performance of mould-proof and mechanical properties. [ABSTRACT FROM AUTHOR]

目的探讨应用集束化护理方案对神经外科气管切开患者肺部感染预防的临床效果。方法选取 2015 年1 月至2016 年12 月该院神经外科收治的100 例颅脑损伤需行气管切开术患者，根据随机原则将其分为对照 组和观察组，每组50 例。对照组患者实施该院神经外科常规的基础方案护理，观察组患者应用集束化护理方案进行护 理干预。观察、比较2 组患者护理干预后肺部感染及并发症发生情况，患者气管切开时间、入住重症监护病房（ICU）时 间及住院总费用等，以及患者和家属对临床护理的满意度。结果观察组患者肺部感染发生率及并发症发生率均明显 低于对照组，患者气管切开时间、入住ICU 时间及住院总费用均明显优于对照组，患者及家属对临床护理的总满意度 明显高于对照组，差异均有统计学意义（P<0.05）。结论对神经外科气管切开患者应用集束化护理方案进行护理干 预，能有效降低患者肺部感染发生率及并发症发生率，缩短患者气管切开时间、入住ICU 时间，明显降低患者住院治疗 总费用，提高患者及家属对临床护理的满意度，其临床应用的综合效果显著，值得进一步推广使用。 [ABSTRACT FROM AUTHOR]