[Objective】 The effects of forest medicine intercropping on soil biochemical properties of walnut forest were analyzed to provide an important theoretical basis for ecological restoration in karst area and sustainable economic development under walnut forest. 【Method】 Four planting modes, walnut intercropping with Mahonia fortunei at initial fruit stage (CT), walnut intercropping with M. fortunei at full fruit stage (ST), walnut monoculture at initial fruit stage (CD), walnut monoculture at full fruit stage (SD) in karst area of Guangxi were used as the research object. Based on sample plot investigation and sampling, the change characteristics and correlation of soil physical and chemical properties, soil microbial quantity, microbial biomass and soil enzyme activity under the intercropping mode of walnut at different forest ages were analyzed. [Result] In CT, contents of soil total nitrogen, total phosphorus, available nitrogen, available phosphorus and organic carbon contents significantly reduced by 43.36%, 40.00%, 32.30%, 67.49% and 43.72% (P<0.05, the same as below), compared with SD mode, ST mode significantly decreased by 28.31%, 46.26%, 17.94%, 53.56% and 28.02%, respectively. The decrease of various soil nutrient contents at initial fruit period was greater than that at full fruit period. The soil nutrient content of walnut at full fruiting stage was significantly higher than that at initial fruiting stage, regardless of intercropping or single cropping. The quantity of soil bacteria, fungi and actinomycetes (12.36 × 106, 5.23 x 104 and 10.87 x 106 CFU/g, respectively) and microbial biomass carbon content (225.87 mg/kg) in SD mode were significantly higher than those in CD and CT modes. Compared with single cropping mode at full fruiting stage and initial fruiting stage, the activities of urease in soil were significantly increased by 17.32% and 40. 13%, and the activities of sucrase, phosphatase and catalase in soil were not significantly affected (P>0.05). However, the activities of sucrase in soil in ST mode were higher than those in CT mode. Correlation analysis showed that the number of soil bacteria was significantly or extremely significantly positively correlated with the contents of soil organic carbon, total nitrogen, total phosphorus and available nitrogen (P<0.01, the same as below). Both soil invertase and phosphatase activity were extremely significantly positively correlated with soil total nitrogen, available nitrogen and organic carbon content. The results of principal component analysis showed that soil nutrients content were the main influencing factors of walnut forest soil, and microorganisms played an important role in promoting soil fertility. [Conclusion] In the karst area, the intercropping mode of M. fortunei in walnut forest has a greater impact on the soil biochemical properties, especially during the initial fruit stage, the soil nutrients in the walnut forest are significantly reduced. So when walnut intercropping with M. fortunei (especially at the initial fruit stage), nitrogen and phosphorus fertilizers should be applied to ensure the nutrition supply of walnut and promote the sustainable development of walnut forest. [ABSTRACT FROM AUTHOR]