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Copyright of Feed Research is the property of Editorial Board of Feed Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To discuss the regulatory effect of serum and glucocorticoid-induced protein kinase 1 (SGK1) in the early development of fertilized eggs at G1 phase of the mice, and to clarify the related mechanism. Methods: Some female mice aged 4-6 weeks and weighed about 20 g, and several male mice aged over 8 weeks and weighed about 30 g were selected. The female mice were intraperitoneally injected with 10 IU of pregnant mare serum gonadotropin (PMSG), followed by 10 IU of human chorionic gonadotropin (HCG) after 48 h. After HCG injection, the female mice were caged overnight with the male mice at a ratio of 1: 1. The fertilized eggs at G1, S, G2, and M phases were collected at 12-21 h, 21-26 h, 26-28 h, and 28-30 h after injected with HCG, and their cellular morphology at different cell cycles were observed under light microscope. The mouse fertilized eggs at G1 phase after superovulation were collected, the mRNA was synthesized in vitro, and divided into no injection group, Tris-EDTA buffer injection group (TE injection group), and SGK1-mRNA injection group. The SGK1 antibodies were mixed with KSOM culture medium with the concentrations of 1 : 25, 1 : 50, 1 : 100, 1: 200, and 0 to culture the mouse fertilized eggs at G1 phase. Western blotting method was used to detect the expression levels of SGK1 protein in fertilized eggs of the mice in various groups and the dephosphorylation for phosphorylated SGK1-Threonine 256 site tyrosine15 site of cell diusion cyclin 2 (Cdc2) (Cdc2-pTyr15) in the fertilized eggs of the mice in various groups and different concentrations of SGK1 antibody groups and the developmental states of the fertilized eggs in the fertilized eggs of the mice in various groups and different concentrations of SGK1 antibody groups were observed under phase contrast microscope; the expression levels of phosphorylated SGK1-Thr256 (SGK1-pThr256) and Cdc2-pTyr15 proteins in fertilized eggs at different post-HCG injection times were detected by Western blotting method. Results: Compared with no injection and TE injection groups, the expression level of SGK1 protein in the cells in SGK1-mRNA injection group was significantly increased (P<0. 01). 27-28 h after injected with HCG, the phosphorylation signaling of Cdc2-pTyr15 in fertilized eggs of the mice in SGK1-mRNA injection group was gradually disappeared, and there was no phosphorylation signaling 29 h after injected with HCG. At 28-29 h after injected with HCG, the phosphorylation signaling of Cdc2-pTyr15 in fertilized eggs of the mice in no injection and TE injection groups gradually disappeared, completely disappeared at 30 h after injected with HCG. With the increasing of the concentration of SGK1 antibody, the disappearing time of the Cdc2-pTyr15 phosphorylation signaling was increased. At 27 h after injected with HCG, the fertilized eggs of the mice in SGK1-mRNA injection group was initiated cleavage; at 31 h after injected with HCG, nearly all the fertilized eggs turned into G2 phase; at 33 h after injected with HCG, all the fertilized eggs in 0 and 1: 200 SGK1 antibody groups underwent cleavage. However, with the increasing of SGK1 antibody concentration, the cleavage of the fertilized eggs in 1: 25, 1: 50, and 1: 100 SGK1 antibody groups was gradually decreased, particularly at 1: 25 SGK1 antibody group. Compared with no injection and TE injection groups, the death rate of the fertilized eggs of the mice in SGK1-mRNA injection group was significantly decreased at 31 h after injected with HCG (P<0. 05), and the cleavage rate was increased (P<0. 05). With the increasing of the SGK1 antibody concentration, the death rates of the fertilized eggs in different concentrations of SGK1 antibody group were increased (P<0. 05), with the extending of cleavage time was increased, and the cleavage rate of the fertilized eggs was decreased in a dose-dependent manner, and the cleavage rate of fertilized eggs in 1: 25 SGK1 antibody group was the lowest. The expression level of SGK1-pThr256 protein in fertilized eggs of the mice was gradually increased from 27 h after injected with HCG (P<0. 05 or P< 0. 01) in a time-dependent manner; at 28 to 29 h after injected with HCG, the expression levels of Cdc2-Tyr15 protein were gradually decreased (P<0. 05) in a time-dependent manner, and had completely disappeared at 30 h after injected with HCG. Conclusion: Both the over-expression and inhibition of SGK1 can affect the time for the fertilized eggs at G1 phase to entry into M phase, suggesting that SGK1 protein may be one of the regulatory factors in the early development of fertilized eggs at G1 phase of the mice, and it may regulate the development of the fertilized eggs at G1 phase through regulation of Cdc2. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to solve the problems of uneven resource utilization and long task completion time in complex task group scheduling, this paper constructed a complex task group resource scheduling model to minimize the mean square error of resource load and the task group completion time, and proposed a multi-objective optimization algorithm based on boundary range local search and NSGA-Ⅱ, called BRLSN. The algorithm used an effective coding method and cross mutation operator for iterative optimization, and constructed an elite retention strategy based on local search in boundary region to expand the search scope of the algorithm and preserved good individuals in the population. Experimental results show that compared with other multi-objective algorithms, the convergence and diversity of BRLSN are significantly improved. At the same time, the algorithm convergence speed is faster, the population quality is higher, and the result value of the final objective function is obviously optimized. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Osteoporosis is the property of Chinese Journal of Osteoporosis and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science Technology & Engineering is the property of Chinese Society of Technology Economics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

For complex driving scenarios such as urban roads, proposed a trajectory planning method for autonomous vehicles based on interactive vehicle trajectory prediction, which decouples high-dimensional trajectory planning into low-dimensional path planning and speed planning. First, used the quintic polynomial curve and the remaining time of the collision to plan the vehicle drive path; Secondly, based on the social generative adversarial network Social-GAN, the trajectory of the interactive vehicle is predicted by combining the vehicle space influence and attention mechanism; Then, combined with the planned path of the main vehicle, the predicted trajectory of the interactive vehicle and the collision determination model to obtain the S-T map of the main vehicle, and used dynamic programming and numerical optimization methods to solve S-T diagram, so as to obtain a safe and comfortable optimal speed curve that satisfies the vehicle dynamics constraints; Finally, build the PreScan-CarSim-Matlab＆Simulink-Python co-simulation model for experimental verification. The simulation results showed that the proposed trajectory planning method can ensure the comfort and efficiency of vehicle driving on the premise of effectively avoiding collisions with interactive vehicles. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Geodesy & Geodynamics (1671-5942) is the property of Editorial Board Journal of Geodesy & Geodynamics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science Technology & Industry / Keji he Chanye is the property of Chinese Society of Technology Economics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The experiment was to investigate the effect of different dietary carbohydrate ratios on ruminal fermentation parameters and serum biochemical index in Liaoning cashmere goats. Fifteen Liaoning cashmere goats with similar body condition, body weight (52.5±2.1) kg and permanent rumen fistula were randomly divided into five groups with three replicates in each group. The goats were fed with SC/NSC of 0.85, 1.05, 1.28, 1.56 and 1.96, respectively. The experimental period lasted for 25 d. The results showed that with the increase of SC/NSC ratios, ruminal fluid pH value increased significantly (P<0.05). The pH value of 0.85 group was significantly lower than that of 1.28 group, 1.59 group and 1.96 group (P<0.05), and pH value of 1.05 group was significantly lower than that of 1.96 group (P<0.05). The concentration of NH3-N in 0.85 group and 1.05 group were significantly higher than that in other groups (P<0.05). With the increase of SC/NSC ratio in diets, VFA concentrations decreased, and the content of acetic acid increased in rumen fluid. The experiment indicates that with the increase of dietary SC/NSC ratios, ruminal pH value and the content of acetic acid increased, VFA and NH3-N concentrations decreased, and low SC/NSC ratio was beneficial to improve carbohydrate utilization efficiency. [ABSTRACT FROM AUTHOR]

Copyright of Feed Research is the property of Editorial Board of Feed Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)