Search

Objective To investigate the serum expression characteristics of miR-27b-3p and miR-342-3p in type 2 diabetic nephropathy (DN) patients and the diagnostic value for DN. Methods A total of 157 patients with DN were selected and divided into the normal albuminuria group (NA group, n=49), the microalbuminuria group (MA group, n=41) and the macroalbuminuria group (DN group, n=67) according to the ratio of albumin to creatinine (UACR) of urine specimens. Clinical data and the expression of serum miR-27b-3p and miR-342-3p, renal function and urinary fibrosis indicators were detected by real-time quantitative polymerase chain reaction. Blood urea nitrogen (BUN) and blood creatinine (Scr), were detected by automatic biochemical analyzer. Urine type Ⅳ collagen (Ⅳ-C), laminin (LN) and type Ⅲ procollagen peptide (PCⅢ) were detected by double-antibody sandwich enzyme-linked immunosorbent assay. The factors influencing the pathogenesis of DN and the diagnostic value of miR-27b-3p and miR-342-3p for DN were analyzed. Results Compared with the NA group, the levels of HbA1c, UACR, urinary Ⅳ-C, urinary LN and urinary PCⅢ were increased, while the expresson levels of miR-27b-3p and miR-342-3p were decreased in the DN group and the MA group (P＜0.05). Levels of BUN and Scr were increased in the DN group (P＜0.05), and eGFR level decreased (P＜0.05). The higher level of urinary PCⅢ was a risk factor for DN, while the higher levels of miR-27b-3p, miR-342-3p and eGFR were protective factors of DN (P＜0.05). The expressions levels of miR-27b-3p and miR-342-3p were positively correlated with eGFR (r = 0.647, 0.661, P＜0.05) and negatively correlated with urinary PCⅢ (r=-0.739, P＜0.05). The area under the curve for diagnosing DN by combining eGFR, urinary PCⅢ, miR-27b-3p and miR-342-3p was higher than that of eGFR, urinary PCⅢ, miR-27b-3p, miR-342-3p alone (Z=5.631, 5.794, 6.715, 4.577, P＜0.05). Conclusion The decreased expressions of serum miR-27b-3p and miR-342-3p are associated with decreased renal function and DN occurrence in patients with type 2 diabetes, which can be used as biological indicators for the auxiliary diagnosis of DN. [ABSTRACT FROM AUTHOR]

Objective: To observe the clinical distribution and drug-resistance of A. baumannii, so as to provide the reference for the rational use of antibiotics in clinic. Methods Data of 2 453 A. baumannii isolated from the clinical samples in a hospital from January 2014 to December 2020 were analyzed retrospectively. Results A total of 2 453 strains of A.baumannii were mainly isolated from the patients in the Intensive Care Unit (35.2%) and surgery department (22.1%). The ages of the patients with A.baumannii detected in the hospital were mainly over 60 years old (59.1%). The detection rate of A. baumannii isolated from the males (8.64%) was significantly higher than that from the females ( 5.39%) ( P < 0.01) . Meanwhile, the detection rate of multidrug-resistant A. baumannii isolated from the males (7.39%) was significantly higher than that from the females (3.58%) (P < 0.01). In addition to Levofloxacin, the resistance rates to Piperacillin, Imipenem, Meropenem, Piperacillin/Tazobactam and Tetracycline were increasing year by year. The isolation rate of multidrug resistant A. baumannii was also increased in the observation period. Furthermore, the drug resistance rate of the imipenem-resistant A. baumannii to 12 kinds of antibacterial drugs, such as Piperacillin, was significantly higher than that of the imipenem-sensitive strains. Conclusion A. baumannii isolated are mainly from the intensive care unit and surgery department. The susceptibility of the male patients to A. baumannii is higher than that of the females. The drug resistance rate of A. baumannii and the isolation rate of multidrug resistant A. baumannii are on the rise and the detection rate of multidrug-resistant A. baumannii is increasing year by year. It is necessary to strengthen the monitoring of A. baumannii and use antimicrobial agents rationally. [ABSTRACT FROM AUTHOR]

To investigate the relationship between serum neutrophil extracellular traps (NETs) and the prognosis of lupus nephritis (LN), and to analyze the prognostic value of NETs in LN. Methods There were 92 patients with LN (the LN group), 97 patients with systemic lupus erythematosus (SLE) and normal renal function (the SLE group), and 83 healthy volunteers (the control group) in this study. According to SLEDAI-2000 score, LN patients were divided into the mild activity group (score≤6, n=31), the moderate activity group (score7-12, n=33), the severe activity group (score＞12, n= 28). According to the occurrence of renal endpoint events during the follow-up period, LN patients were divided into the poor prognosis group (n=25) and the good prognosis group (n=67). Levels of serum NETs were detected to analyze the correlation between NETs and deoxyribonuclease 1 (DNASE1), renal function, SLEDAI-2000 score, renal tissue activity index (AI) and chronic index (CI) scores. Logistic regression was used to analyze the factors affecting the prognosis of LN, and receiver operating characteristic curve (ROC) was used to analyze the prognosis value of NETs in patients with LN. Results The serum level of NETs was higher in the LN group than that of the SLE group and the control group. The serum level of NETs was higher in the severe activity group than that of the moderate activity group and the mild activity group. The serum NETs level was higher in the moderate activity group than that of the mild activity group (P＜0.05). Levels of NETs were negatively correlated with glomerular filtration rate (eGFR) and DNASE1 (r=-0.769), and positively correlated with 24 h urinary protein quantification, SLEDAI-2000 score, renal tissue AI and CI (P＜0.05). High AI score and high NETs were risk factors for poor prognosis of LN (P＜0.05). The area under curve (AUC) of NETs for predicting prognosis in patients with LN was 0.871, with a sensitivity of 80.00% and a specificity of 86.57%. Conclusion Elevated levels of NETs are associated with degree of renal injury and renal end point events in LN, and can be used as a prognostic indicator for LN. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)