Search

Your search keyword '"3D in vitro cancer model"' showing total 4 results
Start Over Descriptor "3D in vitro cancer model"
4 results on '"3D in vitro cancer model"'

2. Bioprinting decellularized breast tissue for the development of three-dimensional breast cancer models

Author

Universitat Politècnica de Catalunya. Doctorat en Ciència i Enginyeria dels Materials, Universitat Politècnica de Catalunya. Doctorat en Enginyeria Biomèdica, Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials, Universitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies, Blanco Fernández, Bárbara, Rey Viñoles, Sergi, Bagci, Gülsün, Rubí Sans, Gerard, Otero, Jorge, Navajas Navarro, Daniel, Pérez Amodio, Soledad Graciela, Engel López, Elisabeth, Universitat Politècnica de Catalunya. Doctorat en Ciència i Enginyeria dels Materials, Universitat Politècnica de Catalunya. Doctorat en Enginyeria Biomèdica, Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials, Universitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies, Blanco Fernández, Bárbara, Rey Viñoles, Sergi, Bagci, Gülsün, Rubí Sans, Gerard, Otero, Jorge, Navajas Navarro, Daniel, Pérez Amodio, Soledad Graciela, and Engel López, Elisabeth

Abstract

The tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipidating a porcine breast tissue (TDM) compatible with hydrogel formation. The addition of gelatin methacrylamide and alginate allows this TDM to be bioprinted by itself with good printability, shape fidelity, and cytocompatibility. Furthermore, this bioink has been tuned to more closely recreate the breast tumor by incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate in both TDM bioinks forming cell clusters and spheroids. The addition of Col1 improves the printability of the bioink as well as increases BCC proliferation and reduces doxorubicin sensitivity due to a downregulation of HSP90. TDM bioinks also allow a precise three-dimensional printing of scaffolds containing BCCs and stromal cells and could be used to fabricate artificial tumors. Taken together, we have proven that these novel bioinks are good candidates for biofabricating breast cancer models., Peer Reviewed, Postprint (published version)

Published
2022

3. Bioprinting decellularized breast tissue for the development of three-dimensional breast cancer models

Author

Barbara Blanco-Fernandez, Sergi Rey-Vinolas, Gülsün Bağcı, Gerard Rubi-Sans, Jorge Otero, Daniel Navajas, Soledad Perez-Amodio, Elisabeth Engel, Universitat Politècnica de Catalunya. Doctorat en Ciència i Enginyeria dels Materials, Universitat Politècnica de Catalunya. Doctorat en Enginyeria Biomèdica, Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials, and Universitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies

Subjects
Three-dimensional printing, Breast tissue, Tissue Engineering, Tissue Scaffolds, Swine, Bioprinting, Enginyeria biomèdica::Biomaterials [Àrees temàtiques de la UPC], Extracellular Matrix, Neoplasms, Printing, Three-Dimensional, Animals, General Materials Science, 3D in vitro cancer model, Impressió 3D, Decellularization
Abstract

The tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipidating a porcine breast tissue (TDM) compatible with hydrogel formation. The addition of gelatin methacrylamide and alginate allows this TDM to be bioprinted by itself with good printability, shape fidelity, and cytocompatibility. Furthermore, this bioink has been tuned to more closely recreate the breast tumor by incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate in both TDM bioinks forming cell clusters and spheroids. The addition of Col1 improves the printability of the bioink as well as increases BCC proliferation and reduces doxorubicin sensitivity due to a downregulation of HSP90. TDM bioinks also allow a precise three-dimensional printing of scaffolds containing BCCs and stromal cells and could be used to fabricate artificial tumors. Taken together, we have proven that these novel bioinks are good candidates for biofabricating breast cancer models.

Published
2022

4. Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models.

Author

Blanco-Fernandez B, Rey-Vinolas S, Bağcı G, Rubi-Sans G, Otero J, Navajas D, Perez-Amodio S, and Engel E

Subjects
Animals, Extracellular Matrix, Printing, Three-Dimensional, Swine, Tissue Engineering methods, Tissue Scaffolds, Bioprinting methods, Neoplasms
Abstract

The tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipidating a porcine breast tissue (TDM) compatible with hydrogel formation. The addition of gelatin methacrylamide and alginate allows this TDM to be bioprinted by itself with good printability, shape fidelity, and cytocompatibility. Furthermore, this bioink has been tuned to more closely recreate the breast tumor by incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate in both TDM bioinks forming cell clusters and spheroids. The addition of Col1 improves the printability of the bioink as well as increases BCC proliferation and reduces doxorubicin sensitivity due to a downregulation of HSP90. TDM bioinks also allow a precise three-dimensional printing of scaffolds containing BCCs and stromal cells and could be used to fabricate artificial tumors. Taken together, we have proven that these novel bioinks are good candidates for biofabricating breast cancer models.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results