163 results on '"A Yu Krylov"'
Search Results
2. The emotive category reflection in Arabic phraseological units and methods of their translation
- Author
-
A Yu Krylov
- Subjects
арабский язык ,эмоции ,категория эмотивности ,экспрессия ,фразеология ,фразеологизмы ,паремии ,перевод ,менталитет ,Philology. Linguistics ,P1-1091 - Abstract
The article deals with one of the most relevant problems of speech - realization of the emotive category in Arabic speech and it also deals with the character of reflection of this category in Arabic phraseological units and the basic methods of their translation into Russian.
- Published
- 2012
3. Association of IRF5 (rs2004640), STAT4 (rs7574865), and TNFAIP3 (rs6920220, rs2230926) gene polymorphisms with primary Sjögren's syndrome and its complication, MALT-lymphoma
- Author
-
I. A. Guseva, A. V. Torgashina, J. I. Khvan, M. Yu. Krylov, E. Yu. Samarkina, N. V. Konovalova, and D. A. Varlamov
- Subjects
primary sjögren's syndrome ,sjögren's syndrome ,malt-lymphoma ,leucopenia ,genetic polymorphisms ,stat4 rs7574865 ,tnfaip3 rs6920220 ,tnfaip3 rs2230926 ,irf5 rs2004640 ,Medicine - Abstract
In recent years, more and more data have emerged confirming the contribution of non-HLA genetic markers to the predisposition to thedevelopment of Sjögren's syndrome (SS) and its severe complication, MALT-lymphoma. Objective: to study the association of polymorphisms of IRF5 (rs2004640), STAT4 (rs7574865), and TNFAIP3 (rs6920220, rs2230926) genes with predisposition to the development of SS and MALT-lymphoma. Materials and methods. The study included 80 patients with SS and 103 individuals in the control group. Sixteen patients were diagnosed with MALT-lymphoma. Genotyping of polymorphisms of IRF5 (rs2004640), STAT4 (rs7574865), TNFAIP3 (rs6920220, rs2230926) genes was performed by real-time polymerase chain reaction using original allele-specific probes labeled with different fluorescent labels. Results and discussion. The distribution of genotypes and alleles of the STAT4 gene differed statistically significantly in the study and control groups of patients (p = 0.0005 and p = 0.0001, respectively). The presence of the homozygous TT genotype increased the risk of developing SS more than eightfold compared to TG+GG genotypes (odds ratio, OR=8.2; 95 % confidence interval, CI 2.5–30.0; p=0.0001)]. The polymorphism of the TNFAIP3 rs2230926 gene was also associated with the risk of developing SS: the presence of the TG genotype significantly increased the probability of developing SS compared to the TT genotype (OR 6.4; 95% CI 1.2–44.3; p = 0.01). The development of MALT-lymphoma was associated with the rs6920220 polymorphism of the TNFAIP3 gene. In 10 out of 16 patients with MALT-lymphoma (62.5 %) at least one minor A allele (AA+GA) was detected, while in patients without MALT-lymphoma only in 32.8 % of patients at least one minor A allele was detected (OR=3.4, CI 1.1–10.7; p=0.03). In addition, a correlation was found between the rs7574865 TT genotype of the STAT4 gene and the risk of developing severe leukopenia in SS, which was significantly more frequent in carriers of the TT genotype than in individuals with the GG + GT genotype (OR 4.9; 95 % CI 1.7–14.4; p = 0.004). Polymorphism of the IRF5 gene (rs2004640) was not associated with risk of developing SS or with clinical manifestations of the disease. Conclusion. Polymorphisms rs7574865 of STAT4 gene, rs6920220, rs2230926 of TNFAIP3 gene are associated either with the risk of developing SS or with severe complications of the disease, MALT-lymphoma and leukopenia.
- Published
- 2023
- Full Text
- View/download PDF
4. Association of TNFAIP3 (rs10499194) and TNF-α (rs1800629) gene polymorphisms with susceptibility to systemic lupus erythematosus with juvenile onset and its clinical phenotypes in the Russian pediatric population
- Author
-
M. Yu. Krylov, M. I. Kaleda, and E. Yu. Samarkina
- Subjects
systemic lupus erythematosus with juvenile onset ,tnfa1p3 gene ,polymorphism rs10499194 ,tnf-α gene ,polymorphism rs1800629 ,clinical phenotype ,arthritis ,Medicine - Abstract
Numerous recent studies have shown that TNFAIP3 and TNF-α gene polymorphisms are associated with susceptibility to certain autoimmune and inflammatory diseases, including systemic lupus erythematosus (SLE), systemic scleroderma, rheumatoid arthritis, psoriasis, etc. However, the results of studies on associations between these polymorphisms and the risk of developing SLE in children are ambiguous and few in number.Objective: to test the hypothesis of a possible association between the rs10499194 polymorphism of the TNFA1P3 gene and the rs1800629 polymorphism of the TNF-α gene with susceptibility to juvenile SLE (jSLE) and its clinical phenotypes in the Russian pediatric population.Material and methods. Both polymorphisms were studied by allele-specific real-time polymerase chain reaction in 63 children (15 boys and 48 girls) with a confirmed diagnosis of jSLE, whose mean age was 12.3±3.2 years (3–17 years), and the mean duration of the disease was 4.1±2.4 years. Data on the frequency of genotypes and alleles of the corresponding TNFA1P3 and TNF-α gene polymorphisms in 309 healthy unrelated blood donors over the age of 18 years (20–45 years) were used as controls.Results and discussion. The study showed that the frequency of the rs10499194T mutant allele of the TNFA1P3 gene in patients with jSLE was significantly lower compared to the control (20.6 and 30.7%; p=0.023), and its carriage slightly reduced the risk of developing SLE (odds ratio, OR 0.58; 95% confidence interval, CI 0.32–1.05, p=0.053). The frequency of the rs1800629A mutant allele of the TNF-α gene was slightly higher in jSLE compared with controls (38.1 and 26.2%, respectively; p=0.056), and its carriage slightly increased the risk of developing SLE (OR 1.73; 95% CI 0.93–3.16; p=0.056). An analysis of the frequency distribution of the rs10499194 genotypes in groups of patients with and without arthritis revealed significant differences (p=0.003). Carrying genotypes with the mutant T allele (CT+TT genotypes) in jSLE significantly reduced the risk of developing of arthritis (p=0.003). At the same time, the risk of arthritis in carriers of at least one C allele was 3.76 times higher than in carriers of the other allele (p=0.006). No relationship was found between the rs1800629 TNF-α gene polymorphism and the clinical phenotypes of jSLE.Conclusion. The rs10499194T mutant allele statistically significant reduces the risk of arthritis development as one of the clinical manifestations of jSLE, and the rs1800629A mutant allele of the TNF-α gene is associated with a tendency to increase the risk of jSLE.
- Published
- 2023
- Full Text
- View/download PDF
5. Prevention and treatment of wound infections after caesarean section
- Author
-
K. Yu. Krylov, N. N. Rukhliada, E. I. Biryukova, and L. Sh. Tsechoeva
- Subjects
caesarean section ,preoperative showering ,surgical knots ,wound infections ,vaginal sanitation ,Surgery ,RD1-811 - Abstract
Caesarean section (cS) is one of the most common obstetric operations in the world. About 32 % of all births end by caesarean section. Postpartum infection at the surgical site, wound infection and endometritis are the main causes of long stays in maternity hospitals and are a source of additional costs for the healthcare system. In 2–16 % of cases postpartum endometritis develops. Many risk factors for the development of postpartum infections have been described. These include maternal factors (such as tobacco use, inadequate prenatal care, obesity, corticosteroid use; prematurity, multiple gestation, and uterine scar), intrapartum and operative factors (such as chorioamnionitis; premature rupture of membranes; prolonged anhydrous period; prolonged labor , especially a long second period; large incision of the anterior abdominal wall; subcutaneous tissue thickness more than 3 cm; subcutaneous hematoma; lack of antibiotic prophylaxis; rapid labor; massive blood loss during childbirth). Effective measures to reduce the incidence of obstetric infectious complications include prophylactic use of antibiotics (preoperative preparation with first-generation cephalosporins and intravenous azithromycin), preoperative showering with chlorhexidine instead of iodine, shaving immediately before surgery, vaginal sanitation, removal of the placenta by traction of the umbilical cord, suturing of the subcutane-ous tissue, if the depth of the wound is larger than 2 cm and suturing the skin with sutures instead of using staples. Establishing an evidence-based optimal care algorithm for patients after caesarean section can reduce the incidence of serious infectious complications.
- Published
- 2023
- Full Text
- View/download PDF
6. USE OF PLASMA CURRENTS IN SURGERY OF PURULENT AND INFLAMMATORY DISEASES
- Author
-
A. M. Shulutko, E. G. Osmanov, F. N. Nasyrov, A. Yu. Krylov, and D. M. Djamalov
- Subjects
plasma currents ,purulent inflammation ,soft tissues ,thrombophlebitis ,treatment ,Medicine (General) ,R5-920 - Abstract
Aim of the study. Evaluation of efficacy of plasma currents in treatment of soft tissue wounds.Materials and methods. 682 patients with purulent inflammation of soft tissues were included into the study: in all of them argon and plasma-air currents were used. The results were compared with 440 patients, who received only standard therapeutic regimens.Results. Use of plasma-air currents was associated with diminishing of frequency of necrectomy procedures, intra-operative blood loss and postoperational pain. Plasma current accelerated necrolysis, regeneration and epythelisation of wound defect, as well as reduction of paravasal inflammation in patients with thrombophlebitis.Conclusion. Plasma current use is effective in treatment of inflammatory diseases of soft tissues.
- Published
- 2022
7. The role of polymorphisms rs12218 of the SAA1 gene, rs1205 of the CRP gene, and rs7574865 of the STAT4 gene in the formation of predisposition to panniculitis in the Russian cohort of patients (pilot study)
- Author
-
M. Yu. Krylov, O. N. Egorova, N. V. Konovalova, and D. A. Varlamov
- Subjects
panniculitis ,erythema nodosum ,idiopathic lobular panniculitis ,polymorphisms ,saa1 gene ,crp gene ,stat4 gene ,Medicine - Abstract
Objective: to study the role of SAA1, CRP and STAT4 gene polymorphisms in the development of panniculitis (PN) and their relationship with clinical and laboratory parameters in the Russian cohort of patients.Patients and methods. The study included 74 patients (67 women and 7 men aged 15 to 76 years) with diagnosis of PN. In addition to the general clinical examination, immunological and histological studies, computed tomography of the chest, and tuberculin tests were performed. For genetic study, two groups of patients were formed: with septal PN (SPN, n=26), represented by erythema nodosum (EN) and with lobular PN (LPN, n=48), including predominantly with idiopathic LPN (iLPN, n= 18) and other rare variants (n=30). As a control, the results of DNA genotyping of 142 healthy non-related individuals were used. Genotyping of polymorphisms rs12218 of the SAA1 gene, rs1205 of the CRP gene, and rs7574865 of the STAT4 gene was performed by the allele-specific real-time polymerase chain reaction.Results and discussion. Significant differences were found between the groups in terms of age and duration of the disease. Patients with SPN were younger than those with LPN (p=0.013), had a shorter duration of the disease (p=0.001), and a lower ESR (p=0.001). Carriers of the TT genotype of the SAA1 gene polymorphism were twice as likely to develop LPN as compared to controls (odds ratio, OR 2.25; 95% confidence interval, CI 1.04–4.87; p=0.038), and this genotype was regarded as a risk factor. For patients with SPN, a significant risk factor was identified in the form of carriage of the mutant TT genotype of the CRP gene polymorphism. This genotype increased the predisposition to the development of EN by 4 times compared with the control (OR 4.39; 95% CI 1.26–14.11; p=0.009). There was a 6-fold increase in the risk of developing EN in carriers of the TT mutant genotype and the T allele of the STAT4 gene polymorphism compared with the control (OR 5.89; 95% CI 1.14–31.75; p=0.016 and OR 2.07; 95 % CI 0.99–4.19, p=0.030, respectively). Comparison of the frequencies of the T allele of the SAA1 gene polymorphism in the groups with EN and with iLPN revealed a higher frequency of the SAA1TT genotype and the SAA1T allele in iLPN than in EN (66.7 and 26.9%, p=0.066; 88.5 and 55.8 %, p=0.016, respectively).Conclusion. The present study confirms the involvement of genetic factors, in addition to generally recognized environmental factors, in the pathogenesis of inflammatory diseases of adipose tissue. Polymorphisms of the SAA1, CRP, and STAT4 genes play a role in the formation of a genetic predisposition to the main clinical phenotypes of PN.
- Published
- 2022
- Full Text
- View/download PDF
8. Association of rs7574865 G/T polymorphism of STAT4 gene with juvenile onset of systemic lupus erythematosus
- Author
-
M. Yu. Krylov, M. I. Kaleda, I. A. Guseva, N. V. Konovalova, and D. A. Varlamov
- Subjects
systemic lupus erythematosus with juvenile onset ,stat4 gene ,polymorphism rs7574865 ,phenotypes of systemic lupus erythematosus ,arthritis ,Medicine - Abstract
Systemic lupus erythematosus (SLE) with juvenile onset (jSLE) is a complex autoimmune disease involving many organs and systems. The single nucleotide polymorphism rs7574865 of the STAT4 (signal switch and transcription activator 4) gene is associated with the risk of developing several autoimmune diseases, including SLE in adults.Objective: to verify the hypothesis of the association of the rs7574865 polymorphism of the STAT4 gene with a predisposition to jSLE. Patients and methods. In the present case-control study, the rs7574865 polymorphism was studied in 50 children with jSLE and 103 healthy control volunteers using real-time polymerase chain reaction.Results and discussion. The distribution of genotype frequencies among patients had statistically significant differences compared to controls (p=0.005). The frequencies of GG, GT, TT, GT + TT genotypes in patients with jSLE and in the control group were 36.0 and 63.1% (p=0.003); 54.0 and 33.0% (p=0.021); 10.0 and 3.9% (p=0.153); 64.0 and 36.9% respectively (p=0.003). The frequency of the mutant T allele of the studied polymorphism was higher in patients with jSLE compared with controls (37 and 20.4%, respectively; p=0.002). The association of the T allele with clinical, laboratory, and immunological phenotypes of jSLE was studied.Conclusion. The obtained data indicate the significance of the rs7574865 polymorphism of the STAT4 gene as an important risk factor for susceptibility to jSLE. An analysis of the distribution of allele frequencies between alternative groups of patients with different phenotypic manifestations of jSLE (their presence or absence) showed an association of this polymorphism with arthritis.
- Published
- 2022
- Full Text
- View/download PDF
9. Atomistic mechanisms for frictional energy dissipation during continuous sliding
- Author
-
S. Yu. Krylov and J. W. M. Frenken
- Subjects
Medicine ,Science - Abstract
Abstract After more than a century of detailed investigations into sliding friction, we have not arrived yet at a basic understanding of energy dissipation, even for the simple geometry of a rigid slider moving over a perfectly periodic counter surface. In this article, we use a first-principles-based analysis to establish the atomistic mechanisms of frictional energy dissipation for a rigid object that moves continuously in the periodic surface potential landscape of a solid with vibrational degrees of freedom. We identify two mechanisms that can be viewed as (i) the continuous pumping of energy into the resonant modes, if these exist, and (ii) the destructive interference of the force contributions introduced by all excited phonon modes. These mechanisms act already in a purely dynamic system that includes independent, non-interacting phonon modes, and they manifest irreversibility as a kind of “dynamical stochastization”. In contrast to wide-spread views, we show that the transformation of mechanical energy into heat, that always takes place in real systems due to the coupling between phonon modes, can play only a minor role in the appearance of friction, if any. This insight into the microscopic mechanisms of energy dissipation opens a new, direct way towards true control over friction.
- Published
- 2021
- Full Text
- View/download PDF
10. Polymorphism rs10499194 of the TNFA1P3 gene is not associated with a predisposition to ankylosing spondylitis in the Russian cohort of patients
- Author
-
M. Yu. Krylov, Sh. F. Erdes, N. V. Konovalova, and D. A. Varlamov
- Subjects
ankylosing spondylitis ,tnfa1p3 gene ,rs10499194 polymorphism ,rs10499194 tt genotype ,gender ,extraaxillary arthritis ,basdai ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Background. Recently, numerous studies have shown that TNFAIP3 gene polymorphisms have been associated with susceptibility to certain autoimmune inflammatory diseases, including systemic lupus erythematosus, scleroderma, rheumatoid arthritis and psoriasis. However, the results of studies devoted to the study of associations between TNFAIP3 gene polymorphisms and the risk of ankylosing spondylitis (AS) are ambiguous and few.The aim of the study was to study the possible association of hs10499194 polymorphism of the TNFAIP3 gene with a predisposition to AS and its clinical phenotypes.Material and methods. The rs10499194 S/T polymorphism of the TNFA1P3 gene was studied in two hundred patients with AS (130 men and 70 women). All patients were diagnosed with AS, according to the modified New York criteria, 1984 and high activity of the disease. Demographic and clinical-serological characteristics were studied in all patients. The average age of patients was 39.4±12.6 years; the average duration of the disease was 15.0±10.6 years. Out of 200 patients, 175 (87.5%) were seropositive for HLA-B27 antigen. Extra axial arthritis was detected in 125 (62.5%) patients, 148 (74.0%) had enthesitis, 137 (68.5%) had coxitis. The polymorphism rs10499194 of the TNFAIP3 gene was studied using an allelespecific polymerase chain reaction in real time (PCR-RV) using the Synthol kit.Results. The analysis of the frequencies of genotypes and alleles did not show significant differences with the control group. Stratification by sex, age, and clinical manifestations showed an association of the CT genotype with an increased risk of AS among men (OR=2.24; p=0.010), the TT genotype and the T allele with a high risk of predisposition to the development of extra axillary peripheral arthritis (OR=3.94; p=0.019 and OR=1.64; p=0.027 respectively). The BASDAI index was statistically significantly higher in carriers of the TT genotype compared to the CT genotype (p=0.002).Conclusion. The present study confirmed the association of the genetic polymorphism rs10499194 of the TNFAIP3 gene with AS. Stratification by gender and clinical manifestations showed an association of the CT genotype with an increased risk of AS among men, the TT genotype and the T allele with a high risk of predisposition to the development of extra axillary peripheral arthritis and a high BASDAI index in carriers of the TT genotype.
- Published
- 2022
- Full Text
- View/download PDF
11. Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method
- Author
-
Maria N. Starodubtseva, Nastassia M. Shkliarava, Irina A. Chelnokova, María I. Villalba, Andrei Yu. Krylov, Eldar A. Nadyrov, and Sandor Kasas
- Subjects
elastic modulus ,viscoelasticity ,nanomotion ,actin cytoskeleton ,cancer cell ,BT-20 cell ,Cytology ,QH573-671 - Abstract
Cells of two molecular genetic types of breast cancer—hormone-dependent breast cancer (ZR-75 cell line) and triple-negative breast cancer (BT-20 cell line)—were studied using atomic force microscopy and an optical nanomotion detection method. Using the Peak Force QNM and Force Volume AFM modes, we revealed the unique patterns of the dependence of Young’s modulus on the indentation depth for two cancer cell lines that correlate with the features of the spatial organization of the actin cytoskeleton. Within a 200–300 nm layer just under the cell membrane, BT-20 cells are stiffer than ZR-75 cells, whereas in deeper cell regions, Young’s modulus of ZR-75 cells exceeds that of BT-20 cells. Two cancer cell lines also displayed a difference in cell nanomotion dynamics upon exposure to cytochalasin D, a potent actin polymerization inhibitor. The drug strongly modified the nanomotion pattern of BT-20 cells, whereas it had almost no effect on the ZR-75 cells. We are confident that nanomotion monitoring and measurement of the stiffness of cancer cells at various indentation depths deserve further studies to obtain effective predictive parameters for use in clinical practice.
- Published
- 2023
- Full Text
- View/download PDF
12. Factors associated with rs1801394 of the methionine synthase reductase gene polymorphism in patients with rheumatoid arthritis
- Author
-
M. Yu. Krylov, G. I. Gridneva, and Yu. V. Muravyev
- Subjects
methotrexate ,mtrr gene ,polymorphism a66g ,das28 ,rheumatoid arthritis ,Medicine - Abstract
Clinical response to methotrexate (MT) therapy in rheumatoid arthritis (RA) can be predicted on the basis of some single nucleotide polymorphisms (SNPs) of genes, involved in folate metabolism. One of these SNPs is the rs1801394 (A66G) polymorphism of the methionine synthase reductase gene (MTRR). We investigated the association of this polymorphism with the clinical characteristics of RA patients after 6 months of MT therapy. Studies of the relationship between the response to MT therapy and the rs1801394 polymorphism have not been carried out in Russia previously.Objective: to study the possible association of the rs1801394 polymorphism with the clinical characteristics of patients with RA after 6 months of MT therapy.Patients and methods. The study included 60 patients with RA who met the ACR / EULAR criteria (2010) and received≥20 mg MT per week continuously. Based on the EULAR criteria, patients were divided into two groups: group 1 (n=30) with a good (DAS28>1.2) and group 2 (n=30) with an unsatisfactory (DAS28
- Published
- 2021
- Full Text
- View/download PDF
13. Relationship of genetic polymorphism of the acute phase marker of inflammation rs12218 of the SAA1 gene with clinical phenotypes of juvenile idiopathic arthritis
- Author
-
M. Yu. Krylov, E. S. Fedorov, and S. O. Salugina
- Subjects
juvenile idiopathic arthritis ,predisposition to jia ,acute phase protein saa ,the saa1 gene ,saa1 gene rs12218 t/c polymorphism ,hla-b27 ,anterior uveitis ,Medicine - Abstract
Objective: to test the hypothesis of a possible relationship between the rs12218 polymorphism of the SAA1 gene and a predisposition to different clinical phenotypes of juvenile idiopathic arthritis (JIA).Patients and methods. Genetic typing of rs12218 polymorphism was carried out in 142 children: 77 of them were diagnosed with JIA, including 30 patients with oligoarthritis (oJIA), 20 with polyarthritis (pJIA), and 27 with systemic onset (sJIA). Sixty five healthy volunteers were included in the control group. The rs12218 polymorphism of the SAA1 gene was investigated using real-time polymerase chain reaction.Results and discussion. A high risk of developing the clinical phenotype of oJIA in carriers of the C mutant allele of the rs12218 T/C polymorphism of the SAA1 gene was established. Statistically significant differences between the clinical phenotypes of oJIA and sJIA in the frequency distribution of genotypes and alleles of rs12218 T/C polymorphism of the SAA1 gene are shown.Conclusion. The results of the studies have confirmed the important role of the rs12218 T/C polymorphism of the SAA1 gene in the formation of susceptibility to clinical variants of JIA.
- Published
- 2021
- Full Text
- View/download PDF
14. STAT4 rs7574865 G/T and IRF5 rs2004640 G/T polymorphisms as markers of predisposition to juvenile idiopathic arthritis. What can genetics give to understand its heterogeneity?
- Author
-
E. S. Fedorov, M. Yu. Krylov, S. O. Salugina, E. Yu. Samarkina, and A. N. Latypova
- Subjects
juvenile idiopathic arthritis ,uveitis ,genetics ,predisposition ,genotyping ,stat4 ,Medicine - Abstract
Juvenile idiopathic arthritis (JIA) is a multifactorial immune-mediated inflammatory disease in childhood, the most common type of rheumatic disease in children. It is characterized by the polygenic type of hereditary predisposition.Objective: to study the association of STAT4 rs7574865 G/T and IRF5 rs2004640 G/T polymorphisms with the predisposition to certain JIA subtypes in the Russian pediatric population.Patients and methods. The investigation enrolled 177 patients, including 66 patients diagnosed with JIA and 111 healthy unrelated volunteers (a control group). Of the 66 patients with JIA there were 30 (45%) with oligoarthritis: 20 (67%) with human leukocyte antigen B27(HLA-B27)-positive JIA (that was associated with enthesitis, HLA-B27 positive JIA (JIA-B27), 10 (33%) with anterior uveitis concurrent with antinuclear antibody-positive JIA (JIA-uveitis); 20 (30%) with polyarticular JIA (JIA-poly), seronegative for rheumatoid factor; and 16 (24%) with systemic JIA (JIA-sys). As a control for genotyping STAT4 rs7574865 G/T and IRF5 rs2004640 G/T polymorphisms, the investigators studied 103 and 111 DNA samples from healthy adult volunteers, respectively. STAT4 rs7574865 G/T and IRF5 rs2004640 G/T polymorphisms were investigated using allele-specific real-time polymerase chain reaction (RT-PCR).Results and discussion. In the oligoarticular JIA group, the frequency of the STAT4 T allele was significantly higher than that in the control group (38.3 and 20.4%, respectively; p=0.004). This allele was also significantly more common in the JIA-B27 (35.0 and 20.4%, respectively; p=0.044) and JIA-uveitis (45.0 and 20.4%, respectively; p=0.021) groups compared with the control one. No significant differences were found in the frequency of the mutant STAT4 T allele between the control group and the JIA-sys and JIA-poly groups. Regression analysis showed that the identification of the STAT4 T allele was associated with the high risk of a predisposition to oligoarticular JIA as a whole (odds ratio, OR 2.43; 95% confidence interval (CI) 1.23–4.70; p=0.007), as well as to the antinuclear antibody-positive oligoarticular JIA with uveitis (JIA-uveitis): the risk in T allele carriers was 3.2 times higher than that in the control (OR 3.19; 95% CI 1.09–9.06; p= ). A high risk for predisposition was also found in the JIA-B27 subgroup compared with the control (OR 2.10; 95% CI 0.38–4.60; p=0.070). There were no statistical differences in the frequency of genotypes and alleles of the IRF5 rs2004640 G/T polymorphism between the entire group of JIA as a whole and its individual clinical types, as well as the control group.Conclusion. This pilot study confirmed that the STAT4 rs7574865 G/T polymorphism was associated with the risk of oligoarticular JIA, mainly that of JIA-uveitis and JIA-B27.
- Published
- 2019
- Full Text
- View/download PDF
15. Association of ankylosing spondylitis activity indicators in a Russian population of patients with STAT4 rs7574865 gene polymorphism
- Author
-
M. Yu. Krylov, A. S. Starkova, E. Yu. Samarkina, T. V. Dubinina, and Sh. F. Erdes
- Subjects
ankylosing spondylitis ,stat4 gene ,rs7574865 polymorphism ,c-reactive protein ,activity indices basdai ,asdas ,Medicine - Abstract
Family and twin studies have shown that ankylosing spondylitis (AS) has a hereditary nature that is based on a strong association with the leukocyte antigen HLA-B27. However, only 1–5% of HLA-B27 carriers develop AS, which indicates that there are other genetic markers involved in the formation of a predisposition to this disease. A number of genome-wide association studies have convincingly confirmed the role of the STAT4 gene. This gene encodes the protein – the signal transducer and activator of transcription (STAT) protein, which is a predisposing factor for the development of many autoimmune diseases. There are not so many studies of the relationship of STAT4 polymorphisms to the predisposition to AS, and there are no these studies regarding the Russian population.Objective: to study whether there is a possible association of STAT4 rs7574865 gene polymorphism with the predisposition to AS and to assess the activity of this disease using BASDAI and ASDAS scores in the Russian patient population.Patients and methods. A cohort of 203 individuals, including 100 patients (79 men and 21 women) with AS, and 103 healthy volunteers (a control group) was surveyed. Age, gender, duration, and specific features of AS onset, ESR, and CRP levels were assessed. BASDAI and ASDAS scores were calculated to evaluate disease activity.Results and discussion. There was a significant relationship between STAT4 polymorphism and C-reactive protein (CRP) levels and BASDAI and ASDAS-CRP scores. The TT genotype carriers had significantly higher mean activity indices compared to the GG (p=0.001) and GT (p=0.005) genotype carriers for CRP, BASDAI (p=0.0001 and p=0.009, respectively) and ASDAS-CRP (p=0.009 and p=0.001, respectively). High disease activity (BASDAI >4 and ASDAS-CRP >3.5) was also associated with the high frequency of the T allele (p=0.046 and p=0.004, respectively). The value of STAT4 rs7574865 gene polymorphism in the pathogenesis of autoimmune diseases is confirmed by a study in which the T allele in STAT4 rs7574865 enhances mRNA transcription and protein expression. Italian authors have shown that there is a relationship between the minor T allele of rs7574865 and the high risk of arthritis. We have previously established a relationship between the T allele and the predisposition to diffuse systemic scleroderma, interstitial lung damage, and elevated anti-topoisomerase I antibody levels.Conclusion. The present study has shown for the first time a significant association of STAT4 rs7574865 polymorphism with the main AS activity indicators: CRP levels, BASDAI and ASDAS-CRP scores. The studied polymorphism may be a new genetic marker for predicting the severity of AS.
- Published
- 2019
- Full Text
- View/download PDF
16. The choice of enteral formula in patients in the acute period of critical ill with brain damage in the neurocritical care unit
- Author
-
Kirill Yu. Krylov, Sergey V. Sviridov, Irina V. Vedenina, and Ruben S. Yagubyan
- Abstract
Nutritional support is an important and integral part of the treatment process for critically ill patients. Patients after neurosurgical interventions and patients with damage to the central nervous system, for example, due to acute ischemic stroke, may be due to the severity of the condition in the intensive care unit. This is a separate category of patients and nutritional support for this category of patients has its own characteristics. Enteral nutrition, as the most physiological type of nutrition, should be an integral part of the treatment process in the intensive care unit. In patients in the acute period of a severe condition with brain damage, in addition to the metabolic response to damage, there are also factors that limit the implementation of enteral nutrition: the brain damage itself, being in intensive care, and methods of intensive therapy. The choice of an enteral formula in this category of patients is complex and fundamental to provide adequate nutritional support to cover energy and protein requirements. The purpose of this scientific review is to highlight the issues of choosing a mixture for enteral nutrition of patients in the neurocritical care unit who are in the acute period of a critical ill.
- Published
- 2023
- Full Text
- View/download PDF
17. Nutritional support as part of the basic therapy of a patient in the acute period of ischemic stroke in the intensive care unit
- Author
-
Kirill Yu. Krylov, Sergey V. Sviridov, Irina V. Vedenina, and Ruben S. Yagubyan
- Abstract
Ischemic stroke remains the predominant cause of disability in the population (3.2 per 1000 population). Only 8% of ischemic stroke survivors can return to their previous work. Nutritional support is an integral part of the multidisciplinary approach to the treatment of ischemic stroke in the intensive care unit during the acute and rehabilitation phases. Brain damage always has metabolic consequences on the patients body, and premorbid malnutrition and stroke-stroke malnutrition significantly affect disease outcomes. The incidence of malnutrition in patients with ischemic stroke varies widely, ranging from 6.1% to 62%, depending on the method for determining protein-energy malnutrition. Despite the relevance of the problem of nutritional support in patients with ischemic stroke, only a few specific recommendations are available in the literature for this category of patients. This review focuses on nutritional support for patients who require mechanical ventilation or admission in the intensive care unit for 48 h.
- Published
- 2023
- Full Text
- View/download PDF
18. Relating interferon regulatory factor 5 rs2004640 gene polymorphism to increased risk of systemic sclerosis in the patients: Russian Federation cohort
- Author
-
Mikhail Yu. Krylov, Lidia P. Ananieva, and Irina A. Guseva
- Subjects
interferon ,irf5 gene polymorphism ,systemic sclerosis ,interstitial lung disease ,Medicine (General) ,R5-920 - Abstract
Background ― A number of studies confirmed a crucial role of type 1 interferon in pathophysiology of connective tissue diseases. Interferon regulatory factors (IRF) coordinate an expression of type 1 interferon, while interferon regulatory factor 5 (IRF5) gene was recently identified as causing predisposition to systemic lupus erythematosus and Sjögren syndrome. The objective of our study was to identify possible association of IRF5 rs2004640 (G/T) single nucleotide polymorphism with systemic sclerosis (SSc). Material and Methods―The study involved 236 individuals, including 105 patients with SSc diagnosis and 131 control individuals from Moscow region. The latter were healthy, unrelated to each other, their genders and ages were matched to those of SSc patients. Allele-specific real-time polymerase chain reaction (PCR) was used to study IFR5 rs2004640 polymorphism. Results ― We detected significantly higher percentage of IRF5 T-allele carriers in all patients (59.5%), those with diffuse cutaneous SSc (67.3%), patients with interstitial lung lesions (62.3%), and those with positive titers of anti-topoisomerase I antibodies (66.3%), compared with control group (46.2%). The odds ratios (OR) were: 1.71 (р=0.00), 2.40 (р=0.0004), 1.93 (р=0.002), and 2.30 (р=0.0008), correspondingly. Conclusion ― The replacement of nucleotide G by T in the IRF5 rs2004640 gene polymorphism was associated with a predisposition to SSc. Our data implied an existence of a novel SSc pathogenetic pathway associated with important role of type 1 interferon in pathophysiology of connective tissue diseases.
- Published
- 2020
- Full Text
- View/download PDF
19. Association of polymorphisms of HLA-DRB1 and TNF-308 G/A with radiographic joint damage in patients with early rheumatoid arthritis with high inflammatory activity, treated according to the principle of 'Treat to target' (REMARKA study)
- Author
-
I A Guseva, A V Smirnov, N V Demidova, M Yu Krylov, A S Avdeeva, E Yu Samarkina, E L Luchikhina, D E Karateev, D D Abramov, and E L Nasonov
- Subjects
hla-drb1 ,shared epitope ,se ,tnfa ,early rheumatoid arthritis ,disease activity ,radiographic damage ,single nucleotide polymorphism ,Medicine - Abstract
Objective. To clarify the association between HLA-DRB1 and TNFα (-308G>A) genes polymorphism and joint destruction/further progression during 12 months of the follow-up period (FUP) in patients with early (
- Published
- 2018
- Full Text
- View/download PDF
20. Prevention and treatment of wound infections after caesarean section
- Author
-
K. Yu. Krylov, N. N. Rukhliada, E. I. Biryukova, and L. Sh. Tsechoeva
- Subjects
General Medicine - Abstract
Caesarean section (cS) is one of the most common obstetric operations in the world. About 32 % of all births end by caesarean section. Postpartum infection at the surgical site, wound infection and endometritis are the main causes of long stays in maternity hospitals and are a source of additional costs for the healthcare system. In 2–16 % of cases postpartum endometritis develops. Many risk factors for the development of postpartum infections have been described. These include maternal factors (such as tobacco use, inadequate prenatal care, obesity, corticosteroid use; prematurity, multiple gestation, and uterine scar), intrapartum and operative factors (such as chorioamnionitis; premature rupture of membranes; prolonged anhydrous period; prolonged labor , especially a long second period; large incision of the anterior abdominal wall; subcutaneous tissue thickness more than 3 cm; subcutaneous hematoma; lack of antibiotic prophylaxis; rapid labor; massive blood loss during childbirth). Effective measures to reduce the incidence of obstetric infectious complications include prophylactic use of antibiotics (preoperative preparation with first-generation cephalosporins and intravenous azithromycin), preoperative showering with chlorhexidine instead of iodine, shaving immediately before surgery, vaginal sanitation, removal of the placenta by traction of the umbilical cord, suturing of the subcutane-ous tissue, if the depth of the wound is larger than 2 cm and suturing the skin with sutures instead of using staples. Establishing an evidence-based optimal care algorithm for patients after caesarean section can reduce the incidence of serious infectious complications.
- Published
- 2022
- Full Text
- View/download PDF
21. Results of immunohistochemistry diagnostics of breast cancer in the region of Grodno with consideration of the BRCA1 gene mutation in triple negative cancer
- Author
-
A. Yu. Krylov, M. G. Zubritsky, I. A. Kurstak, S. A. Lialikau, and V. A. Basinski
- Subjects
immunohistochemical study ,breast cancer ,triple negative breast cancer ,brca1 mutation ,Medicine - Abstract
Background: The results of immunohistochemical (IHC) assessment in breast cancer may determine not only the outcome, but also the main directions of antitumor treatment.Aim: To study the results of IHC diagnosis of breast cancer in the Grodno region in 2010 to 2015, to determine the frequency of the BRCA1 gene mutations in triple-negative breast cancer.Materials and methods: 2008 cases of estrogen receptor and/or progesterone receptor positive and 2445 cases of HER2/neu expressing breast cancer were extracted from the database and IHC laboratory journals of the Grodno Regional Clinical Bureau for Pathological Anatomy. Standard DAKO kits (Denmark) were used for IHC. Molecular genetic studies of DNA samples for the presence of the BRCA genes mutations were done by polymerase chain reaction in 449 women with breast cancer. BRCA1 mutation was assessed in 39 cases of triple-negative breast cancer.Results: HER2/neu overexpression (3+) was found in 25.5% (622/2445) of all breast cancer cases, whereas of estrogen receptor and progesterone receptor positive tumors in 50.05% (1105/2008) of cases. Triple negative breast cancer was diagnosed in 19.8% (398/2008) of cases, and the mean age of the patients at the diagnosis was 56.2 years. The BRCA1 gene mutations were identified in 5 of 39 (12.8%) cases of triple negative breast cancer, which is significantly more often than in women with breast cancer without consideration of their hereditary predisposition (17/449, or 3.8%; χ2 = 4.87, p = 0.0274). Histological examination showed that among the tumors with the BRCA1 mutations, there were 3 cases with invasive ductal cancer (one G2 and two G3), one with medullary and one with undifferentiated G3 cancer. One patient had a metachronous bilateral lesion.Conclusion: In the region of Grodno, 50% of breast cancer patients would need hormonal therapy, 25% would need treatment with trastuzumab, and 20% with cytostatic agents. The prevalence of the BRCA1 mutation in triple negative breast cancer is higher compared to that in breast cancer patients without consideration of their hereditary predisposition.
- Published
- 2017
- Full Text
- View/download PDF
22. The influence of STAT4 rs7574865 (G/T) polymorphism on the risk of clinical and immunological phenotypes of systemic sclerosis in a Russian patient population: Results of a pilot study
- Author
-
M Yu Krylov, L P Ananyeva, О А Koneva, M N Starovoytova, O V Desinova, O B Ovsyannikova, E N Aleksandrova, A A Novikov, I A Guseva, N V Konovalova, and D A Varlamov
- Subjects
systemic sclerosis ,genetics ,polymorphism ,stat4 gene ,population ,Medicine - Abstract
Aim. To examine the association of signal transducer and activator transcription 4 (STAT4) rs7574865 G/T polymorphism with a predisposition to systemic sclerosis (SSC) and associated clinical and autoimmune phenotypes in a Russian population. Subjects and methods. A total of 102 patients with SSC and 103 healthy individuals as controls were examined. STAT4 rs7574865 polymorphism was investigated by real-time polymerase chain reaction. Results. The carriers of the T allele showed a statistically significant association with SSC, a diffuse form (DF), the presence of interstitial lung disease (ILD), cardiac injury (CI), and seropositivity for anti-topoisomerase I antibodies (ATA). Conclusion. The findings results confirm the important role of STAT4 gene in the predisposition to SSC and its phenotypes, such as DF, ILD, CI, and ATA in the Russian population.
- Published
- 2017
- Full Text
- View/download PDF
23. Experience in organizing a clinical nutrition and metabolism laboratory and creating a nutritional support system in the ICU and intensive care departments
- Author
-
Kirill Yu. Krylov, Marina V. Petrova, Alexander E. Shestopalov, Alexandra V. Yakovleva, and Alexey A. Yakovlev
- Abstract
In the intensive care unit, along with other methods of intensive care, such as maintaining blood pressure, antibiotic therapy, mechanical ventilation, and vasopressors, clinical nutrition should always be present. Clinical nutrition is an integral part of the treatment process in the intensive care unit. A large number of publications concerning the prevention and treatment of intensive care outcome syndrome exist. One of the most important and low-cost strategies to prevent this condition is nutritional support for critically ill patients at all stages of curation, from the moment of admission to the intensive care unit to the end of the rehabilitation course. With the correct appointment of nutritional support, taking into account the indications and contraindications for various nosological forms, the advantages are obvious in the form of improved treatment outcomes. Proper organization of nutritional support in the hospital is the main key to success in early rehabilitation. From the understanding of nutritional support, its effectiveness will depend on its organization and mutual understanding between all links. Based on the experience of creating a laboratory for clinical nutrition and metabolism, it can be concluded that the creation of such a laboratory and the organization of clinical nutrition in a hospital can improve the results of treatment of even the most severe category of patients.
- Published
- 2022
- Full Text
- View/download PDF
24. About the current situation with regard to the risks and prevention of venous thromboembolic complications after varicose vein surgery
- Author
-
V. Yu. Bogachev, K. V. Lobastov, A. A. Fokin, E. V. Shaydakov, E. P. Burleva, A. Yu. Krylov, V. E. Barinov, S. M. Belentsov, V. V. Soroka, R. A. Bredikhin, D. A. Rosukhovski, D. A. Borsuk, and P. G. Gabay
- Published
- 2022
- Full Text
- View/download PDF
25. Involvement of polymorphisms of TNF, CRP and SAA1 genes in the predisposition to the development of ankylosing spondylitis and its clinical manifestations
- Author
-
M. Yu. Krylov, I. A. Guseva, K. V. Sakharova, E. Yu. Samarkina, S. F. Erdes, N. V. Konovalova, and D. A. Varlamov
- Subjects
Rheumatology ,Immunology ,Immunology and Allergy - Abstract
Objective. To study the involvement of the rs1800629 G/A polymorphisms of the TNF-α gene, rs1205C/T of the CRP gene, and rs12218T/C of the SAA1 gene in the predisposition to ankylosing spondylitis (AS) and their role in the formation of clinical phenotypes of AS.Materials and methods. 122 patients with AS were included in the study. All patients had a diagnosis of AS based on the modified New York criteria. The presence of the HLA-B27 antigen was detected in 109 (89.3%) patients, the presence of peripheral arthritis – in 71 (58.2%), enthesitis – in 92 (75.4%), coxitis – in 82 (67.2%) patients. All patients had a high degree of activity with an average BASDAI index of 5.6±1.2. The control group consisted of 142 healthy blood donors. Polymorphisms were studied using allele-specific real-time polymerase chain reaction (RT-PCR).Results. Significant differences were found in the frequencies of genotypes and alleles of the -308G/A polymorphism of the TNF gene and the frequencies of alleles of the rs12218 T/C polymorphism of the SAA1 gene between patients and the control group (p=0.01, p=0.01 and p=0.03 respectively). Logistic regression analysis showed that the presence of at least one -308A allele in the patient’s genotype reduced the risk of developing AS by 4.4 times compared with the GG genotype (p=0.006). In carriers of the GA genotype, the probability of a predisposition to the development of enthesitis was 2.2 times lower than in carriers of the GG genotype (p=0.01). The relationship between the rs1205 polymorphism of the CRP gene and the predisposition to peripheral arthritis has been established. Carriage of the rs1205T allele doubled the susceptibility to arthritis compared with the rs1205C allele (p=0.013). Carriage of at least one rs12218C allele of the SAA1 gene doubled the susceptibility to AS (p=0.018).Conclusion. The data obtained confirm the involvement of polymorphisms rs1800629 of the TNF gene, rs1205 of the CRP gene, and rs12218 of the SAA1 gene in the predisposition to AS. TNF gene polymorphism is associated with the formation of the clinical phenotype of enthesitis, and CRP gene polymorphism is associated with a predisposition to peripheral arthritis.
- Published
- 2022
- Full Text
- View/download PDF
26. Resolution of the professional community of phlebologists regarding the examination of patients before minimally invasive interventions for varicose veins of the lower extremities
- Author
-
E. V. Shaydakov, Denis Borsuk, A. A. Fokin, P. G. Gabay, A. Yu. Krylov, E. P. Burleva, Kirill Lobastov, V. V. Soroka, S. M. Belentsov, R. A. Bredikhin, D. A. Rosukhovski, H. P. Manjikian, Victor Barinov, M. Yu. Gilyarov, and V. Yu. Bogachev
- Subjects
medicine.medical_specialty ,RD1-811 ,Radiofrequency ablation ,business.industry ,General surgery ,medicine.medical_treatment ,Psychological intervention ,Legislation ,Medical law ,law.invention ,law ,Varicose veins ,medicine ,Sclerotherapy ,Christian ministry ,Surgery ,medicine.symptom ,business ,Professional community - Abstract
To date, modern outpatient minimally invasive interventions for varicose veins, such as endovenous laser or radiofrequency ablation, microphlebectomy, sclerotherapy, non-thermal non-tumescent methods (NTNT), are widely introduced into clinical practice in the Russia. In the approved by the Ministry of Health of the Russian Federation in 2021 clinical guidelines "Varicose veins of the lower extremities" they are recommended as preferred over open surgery. At the same time, there are no federal regulations of preoperative examination of patients before these minimally invasive procedures for varicose veins. That is why the medical community is often forced to prescribe unnecessary diagnostic tests on the "just-in-case" principle, in order to protect itself for cases of complications and subsequent checks. This undoubtedly harms both the patients and the medical service, which has an additional useless and inappropriate burden. The purpose of the round table held on September 28, 2021 under the auspices of the Self-Regulatory Organization “The National College of Phlebology” together with the legal partner “The Faculty of Medical Law LLC” (Moscow), was to review the existing legislation on this issue and then to prepare a resolution regarding the justified preoperative examination of patients before minimally invasive interventions for varicose veins of the lower extremities. The prepared document consists of two parts. The first part presents the legal regulation of the diagnostic tests before interventions in phlebology. The second part contains the opinion of the professional community regarding the appropriateness or inexpediency of different pre-procedural examinations. In addition, based on the conclusions of legal analytics, a form of order of the chief physician has been developed, which regulates the diagnostic tests before the considered phlebological interventions in the clinic.
- Published
- 2022
27. Thrombodynamics-A new global hemostasis assay for heparin monitoring in patients under the anticoagulant treatment.
- Author
-
Anna N Balandina, Ilya I Serebriyskiy, Alexander V Poletaev, Dmitry M Polokhov, Marina A Gracheva, Ekaterina M Koltsova, David M Vardanyan, Irina A Taranenko, Alexey Yu Krylov, Evdokiya S Urnova, Kirill V Lobastov, Artem V Chernyakov, Elena M Shulutko, Andrey P Momot, Alexander M Shulutko, and Fazoil I Ataullakhanov
- Subjects
Medicine ,Science - Abstract
BACKGROUND:Heparin therapy and prophylaxis may be accompanied by bleeding and thrombotic complications due to individual responses to treatment. Dosage control based on standard laboratory assays poorly reflects the effect of the therapy. The aim of our work was to compare the heparin sensitivity of new thrombodynamics (TD) assay with sensitivity of other standard and global coagulation tests available to date. STUDY POPULATION AND METHODS:A total of 296 patients with high risk of venous thromboembolism (deep vein thrombosis (DVT), early postoperative period, hemoblastosis) were enrolled in the study. We used a case-crossover design to evaluate the sensitivity of new thrombodynamics assay (TD) to the hemostatic state before and after unfractionated heparin (UFH) and low-molecular-weight heparin (LMWH) therapy/prophylaxis and to compare it with the activated partial thromboplastin time (APTT), anti-Xa activity test, thrombin generation test (TGT) and thromboelastography (TEG). A receiver operating characteristic (ROC) curve analysis was used to evaluate changes before and after heparin prophylaxis and therapy. Blood was sampled before heparin injection, at the time of maximal blood heparin concentration and before the next injection. RESULTS:Hypercoagulation before the start of heparin treatment was detected by TD, TGT and TEG but not by APTT. The area under the ROC curve (AUC) was maximal for TD and anti-Xa, intermediate for TGT and TEG and minimal for APTT. CONCLUSIONS:These results indicate that TD has a high sensitivity to the effects of UFH and LMWH after both prophylactic and therapeutic regimes and may be used for heparin monitoring.
- Published
- 2018
- Full Text
- View/download PDF
28. Comment on 'Frictional Energy Dissipation due to Phonon Resonance in Two-Layer Graphene System'
- Author
-
S. Yu. Krylov
- Subjects
Mechanics of Materials ,Mechanical Engineering ,Surfaces and Interfaces ,Surfaces, Coatings and Films - Published
- 2022
- Full Text
- View/download PDF
29. Influence of nodular and severe forms of diffuse adenomyosis on reproductive function: a review of reproductive outcomes of surgical interventions and IVF
- Author
-
K. Yu. Krylov, N. N. Rukhliada, E. I. Biryukova, L. S. Tsechoeva, and S. V. Vinnikova
- Subjects
Infertility ,medicine.medical_specialty ,reproductive outcome ,surgical treatment ,Science ,medicine.medical_treatment ,media_common.quotation_subject ,Fertility ,Reproductive technology ,General Biochemistry, Genetics and Molecular Biology ,Intracytoplasmic sperm injection ,Recurrent miscarriage ,medicine ,Adenomyosis ,media_common ,assisted reproductive technologies ,In vitro fertilisation ,Reproductive function ,General Immunology and Microbiology ,Obstetrics ,business.industry ,medicine.disease ,adenomyosis ,pregnancy ,infertility ,business - Abstract
This review includes an analysis of clinical studies evaluating the effect of adenomyosis on reproductive function, as well as a review of studies on the relationship between surgical treat-ment options for adenomyosis and reproductive outcomes. Different studies of women populations after in vitro fertilization and/or intracytoplasmic sperm injection (IVF/ICSI) and after surgical treatment of deep adenomyosis have shown that adenomyosis in both cases has a negative effect on the reproductive outcome, although there are significant differences in these populations. In the literature, there are relatively few data on the degree of influence of the depth of the lesion on the realization of the reproductive function, however, a significant correlation was revealed between them. The positive effect of treatment with gonadotropin-releasing hormone agonists, followed by surgical treatment of adenomyosis, has also been described in several literary sources, but there have been no controlled studies on this topic. Many studies do not meet the validity criteria due to the lack of clear criteria for inclusion and exclusion from the study. The choice of the optimal treatment options for adenomyosis based on the actual data is difficult due to the lack of reliable evidence of the relationship between fertility and the degree of adenomyosis. However, it is known that adenomyosis can reduce the likelihood of successful implantation so ramatically that surgical or other treatment options are reasonably recom-mended. At the same time, it is necessary to assess the possible positive effect of the treatment options available in the doctor’s arsenal. Thus, timely referral to specialized treatment of ade-nomyosis may be crucial for the successful implementation of reproductive function in patients with suspicion or with an established diagnosis of adenomyosis in combination with recurrent miscarriage, who refuse to use assisted reproductive technologies, medical institutions. This review will analyze the relationship between infertility and adenomyosis, as well as review studies of reproductive outcomes in different options for surgical treatment of adenomyosis in infertile patients.
- Published
- 2021
- Full Text
- View/download PDF
30. Association of the rs2167270 polymorphism of the leptin gene (LEP) with the intensity of pain in patients with osteoarthritis of the knee
- Author
-
M. Yu. Krylov, L. I. Alekseeva, and E. P. Scharapova
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Visual analogue scale ,Physiology ,Endocrinology, Diabetes and Metabolism ,polymorphism rs2167270 lep gene ,Adipokine ,Osteoarthritis ,QD415-436 ,Gastroenterology ,Biochemistry ,knee osteoarthritis ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Pain assessment ,Internal medicine ,Genotype ,Internal Medicine ,medicine ,QP1-981 ,030203 arthritis & rheumatology ,Nutrition and Dietetics ,pain, obesity ,business.industry ,Leptin ,Public Health, Environmental and Occupational Health ,medicine.disease ,bmi ,030104 developmental biology ,Knee pain ,Gene polymorphism ,medicine.symptom ,business - Abstract
Background: Osteoarthritis (OA) is a significant social problem as it is the most common disease of the joints. OA is a multifactorial disease in which great attention is paid to hereditary factors. Recently, a number of studies have demonstrated the contribution of a number of genes to the subjective assessment of pain in OA, which is the main symptom of this disease. The association of P2X7, TRPV1 and TACR1 genes and some others with pain sensitivity has been shown. One of the risk factors of pain among many others, is the increased weight. Abdominal adipose tissue is a source of release of pro-inflammatory adipokines that cause systemic inflammation associated with damage to many tissues, including subchondral bone, synovial membrane. Leptin is an endogenous hormone from the adipokine family encoded by the obesity gene leptin (LEP) and which is synthesized primarily in adipocytes.Aims: To investigate the possible association of rs2167270 (A19G) polymorphism of the LEP gene with pain intensity in patients with knee OA.Materials and methods: The study was conducted among women diagnosed with OA. Using the VAS scale (Visual analog scale), patients with mild knee pain — group 1 (VAS ≤ 40 mm) and patients with moderate or severe pain — group 2 (VAS>40 mm) were selected for pain assessment. Genetic variants of A19G leptin gene polymorphism were studied by polymerase chain reaction followed by restriction fragment length analysis (PCR-RFLP) method.Results: In the group of patients with moderate or severe pain intensity (group 2, n=61), a statistically significant association was shown with a higher body mass index (p=0.006) and an increased frequency of carriers of the 19GG genotype (p=0,051) compared to group 1 (n=36). Carriers of the 19GG genotype statistically significantly had a higher rate of knee pain and an early age of OA debut compared to carriers of the 19AA genotype (p=0,035 and p=0,015, respectively).Conclusions: The findings open up new possibilities for predicting pain symptoms in patients with knee OA by genetic testing of A19G polymorphic variants of the leptin gene.
- Published
- 2021
31. Factors associated with rs1801394 of the methionine synthase reductase gene polymorphism in patients with rheumatoid arthritis
- Author
-
G. I. Gridneva, Yu. V. Muravyev, and M. Yu. Krylov
- Subjects
rheumatoid arthritis ,0301 basic medicine ,polymorphism a66g ,Immunology ,Pharmacology ,methotrexate ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Immunology and Allergy ,Medicine ,Pharmacology (medical) ,In patient ,biology ,mtrr gene ,business.industry ,(Methionine synthase) reductase ,medicine.disease ,das28 ,030104 developmental biology ,030220 oncology & carcinogenesis ,Rheumatoid arthritis ,biology.protein ,Gene polymorphism ,business - Abstract
Clinical response to methotrexate (MT) therapy in rheumatoid arthritis (RA) can be predicted on the basis of some single nucleotide polymorphisms (SNPs) of genes, involved in folate metabolism. One of these SNPs is the rs1801394 (A66G) polymorphism of the methionine synthase reductase gene (MTRR). We investigated the association of this polymorphism with the clinical characteristics of RA patients after 6 months of MT therapy. Studies of the relationship between the response to MT therapy and the rs1801394 polymorphism have not been carried out in Russia previously.Objective: to study the possible association of the rs1801394 polymorphism with the clinical characteristics of patients with RA after 6 months of MT therapy.Patients and methods. The study included 60 patients with RA who met the ACR / EULAR criteria (2010) and received≥20 mg MT per week continuously. Based on the EULAR criteria, patients were divided into two groups: group 1 (n=30) with a good (DAS28>1.2) and group 2 (n=30) with an unsatisfactory (DAS28 Results and discussion. The frequency distribution of the A66G polymorphism genotypes in both groups was similar, however, in the 2nd group with an unsatisfactory response, there was a tendency towards a higher frequency of the mutant GG genotype (p=0.067). An association of the A66G polymorphism with gender and disease duration was found. In group 1, the AG genotype was more often detected in men than in women (p=0.017). In group 2, the AG genotype was also more common in men (p=0.075). In addition, in this group, carriers of the G allele (genotypes AG and GG) had a longer duration of the disease than carriers of the AA genotype (p=0.003 and p=0.005, respectively).Conclusion. In the present study, the relationship of the studied polymorphism rs1801394 of the MTRR gene with gender and duration of RA disease was established.
- Published
- 2021
- Full Text
- View/download PDF
32. Polyacrylamide mammary syndrome — specificity of the histological pattern of the focus before and after air-plasma treatment
- Author
-
Elkhan G. Osmanov, Aleksandr M. Shulutko, Aleksey Yu. Krylov, Elena G. Gandybina, and Anastasiya Ya. Malyugina
- Subjects
General Medicine - Abstract
BACKGROUND: At the end of the twentieth century, mammary gland contour correction using polyacrylamide gel (PAAG) became widely popular. The surgical approach must be improved because of the negative consequences associated with this technique. AIM: This study aimed to evaluate the possibilities of air-plasma technology in the treatment of long-term septic manifestations of polyacrylamide mammary syndrome as well as the prevention of its recurrence based on the analysis of histological examination findings. MATERIALS AND METHODS: Biopsy samples of eight women aged 38 to 59 years, who had undergone gel augmentation of the mammary glands six to 20 years ago, were studied. The complex treatment included air-plasma flow in cutting coagulation modes and nitric oxide therapy. A morphological study of the focus was performed before and after exposure to the plasma flow. RESULTS: Before treatment, chronic granulomatous inflammation and helioma were revealed in six cases; and acute phlegmonous-necrotic soft tissue inflammation in two cases. On the fifth day after combined necrectomy, single loci of the gel were visualized, the majority of which was vaporized. Chronic inflammation was less pronounced than the original histograms. During the sanitation with nitric oxide, the early appearance of foci of fibroblastic proliferation and marginal epithelialization was registered. CONCLUSIONS: Exposure to the air-plasma flow contributes to effective sanitation of purulent focus and gel total elimination Nitric oxide therapy (NO-therapy) accelerates the development of granulation tissue.
- Published
- 2021
- Full Text
- View/download PDF
33. Association of rs7574865 polymorphism of the STAT4 gene with spinal syndesmophytes in patients with ankylosing spondylitis
- Author
-
M. Yu. Krylov and Sh. F. Erdes
- Subjects
musculoskeletal diseases ,RC925-935 ,ankylosing spondylitis ,stat4 gene polymorphism ,Diseases of the musculoskeletal system ,syndesmophytes - Abstract
Objective. The aim of the investigation was to study the possible association of the rs7574865 polymorphism of the STAT4 gene with syndesmophytes (SMP) of the spine in patients with ankylosing spondylitis (AS).Subjects and methods. The study included a cohort of 100 patients, 79 men and 21 women with a diagnosis of AS.All patients were positive for the HLA-B27 antigen, had a mean age of 39.6±10.9 years and a mean disease duration of 60,4±28,4 months. The association of the rs7574865 polymorphism of the STAT4 gene with the SMP of the cervical, thoracic and lumbar spine was studied. For genotyping of the rs7574865 polymorphism, the method of allele-specific polymerase chain reaction in real time (RT-PCR) was used.Results. Spearman’s correlation analysis showed a statistically significant positive relationship between SMP in the thoracic spine and rs7574865 polymorphism of the STAT4 gene (r=0.23; p=0.022). The frequency of GT genotype carriers in the group of patients with thoracic spine trSMP(+) was statistically significantly lower than in the alternative group trSMP(–) (28.2% and 50.8%, respectively; p=0.025). Carriage of the GT genotype in patients with AS reduced the risk of trSMP(+) formation in the thoracic spine (OR=0.31) and this genotype was protective. No reliably significant association of the studied polymorphism with SMP of the cervical and lumbar spine was found. Patients with trSMP(+) were statistically significantly older in age, had a longer duration of the disease and a higher functional BASFI index compared with patients without trSMP(–).Conclusion. Genetic testing of the rs7574865 G/T polymorphism of the STAT4 gene in patients with AS opens up the possibility of using this polymorphism as a genetic marker-predictor – X-ray progression of structural changes in the thoracic spine.
- Published
- 2021
34. Malnutrition in hospitalized older patients. Clinical case
- Author
-
N. K. Runikhina, K. Yu. Krylov, V. S. Ostapenko, and M. M. Balaeva
- Subjects
0301 basic medicine ,Pediatrics ,medicine.medical_specialty ,030109 nutrition & dietetics ,business.industry ,medicine.disease ,03 medical and health sciences ,Malnutrition ,0302 clinical medicine ,Older patients ,Media Technology ,medicine ,030212 general & internal medicine ,Clinical case ,business - Abstract
Malnutrition is a common geriatric syndrome, which often undiagnosed, leads to impaired physical and mental functioning and patient prognosis. Geriatric patients admitted to hospital with acute and subacute conditions and pain syndrome are in an especial risk zone. The article presents a clinical case of the development and correction of malnutrition in an 84-year-old patient with aseptic necrosis of the femoral head. Risk factors and existing approaches to the management of patients with malnutrition are discussed.
- Published
- 2021
- Full Text
- View/download PDF
35. Russian clinical practice guidelines for the management of c1 clinical class of chronic venous disorders (reticular veins and telangiectasias)
- Author
-
S. N. Kornievich, V. S. Martynov, Kirill Lobastov, E. Y. Berezhnoi, O. V. Dzenin, D. A. Rosukhovski, P. A. Pirozhenko, A. V. Albitskii, K. S. Suvorov, I. V. Doronin, H. P. Manjikian, R. A. Bredikhin, A. Yu. Semenov, A. R. Khafizov, V. E. Denisov, S. D. Zokirkhonov, V. N. Lobanov, R. G. Chabbarov, A. A. Fokin, A. S. Sharipov, O. A. Shonov, E. N. Glagoleva, S. M. Belentsov, A. V. Krylov, M. A. Karev, Y. V. Savinova, V. V. Soroka, E. V. Shaydakov, D. N. Morenko, O. O. Ivanov, P. Yu. Turkin, E. O. Belyanina, A. M. Terekhov, Denis Borsuk, E. P. Burleva, N. R. Arkadan, K. A. Kaperiz, A. Yu. Krylov, S. I. Larin, V. Yu. Bogachev, V. V. Raskin, B. V. Boldin, Victor Barinov, Anton S. Dvornikov, O. N. Guzhkov, S. V. Sapelkin, S. I. Pryadko, and E. A. Letunovsky
- Subjects
telangiectasias ,Class (computer programming) ,medicine.medical_specialty ,RD1-811 ,business.industry ,General Medicine ,030204 cardiovascular system & hematology ,Dermatology ,percutaneous laser ablation ,Clinical Practice ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Reticular connective tissue ,sclerotherapy ,Medicine ,Surgery ,chronic venous disorders ,business ,clinical practice guidelines ,reticular veins - Abstract
Recently collated scientific data on the management of C1 clinical class of chronic venous disorders; wide prevalence of the disease and high variability amongst medical practitioners in relation to managing this category of patients and absence of any regulatory documents has prompted the development of clinical guidelines for the treatment of patients with reticular varicose veins and telangiectasias of the lower extremities and various parts of the body. These guidelines have been developed by a self-regulated organization Association “The National College of Phlebology”. The purpose of the de novo guidelines is to systematize the existing evidence and offer minimal standards of care for chronic venous disorders in C1 patients.
- Published
- 2021
36. Home clinical nutrition and prospects for its development
- Author
-
K. Yu. Krylov, A. E. Shestopalov, Sergey V. Sviridov, Marina V. Petrova, V. V. Zozulya, Ruben S. Yagubyan, and M. Rubanes
- Subjects
0301 basic medicine ,medicine.medical_specialty ,030109 nutrition & dietetics ,business.industry ,030209 endocrinology & metabolism ,Clinical nutrition ,Routine practice ,After discharge ,03 medical and health sciences ,0302 clinical medicine ,Family medicine ,medicine ,Russian federation ,Observational study ,business - Abstract
There is an increase in the number of patients worldwide who require special home feeding after discharge from the hospital Home clinical nutrition (HCN). The yearly prevalence of home clinical nutrition in 2017 was at 463 cases per million in the United States of America whereby in the 90s it was around 163360 per million in the United States of America and Europe. There isnt an established practice of HCN in the Russian Federation and thereby no data on the amount of patients who require HCN and the new risks associated with it because there isnt prior experience in conducting it. To study the routine practice and identify patients who need HCN, we planned a multicenter observational monitoring programme. The goal of the study is patient monitoring who are unable to completely cover their energy needs with the help of conventional nutrition.
- Published
- 2020
- Full Text
- View/download PDF
37. Nutritional support for patients with COVID-19 coronavirus infection
- Author
-
Sergey V. Sviridov, A. A. Ryk, D. B. Nikityuk, E. V. Kryukov, Kh. Kh. Sharafetdinov, V. M. Luft, A. E. Shestopalov, I. Yu. Tarmayeva, D. N. Protsenko, Marina V. Petrova, A. V. Starodubova, A. V. Grechko, E. A. Evdokimov, K. Yu. Krylov, V. V. Stets, Alexandra V. Yakovleva, T. S. Popova, A. V. Pogozheva, V. A. Tutelyan, O. N. Kotenko, and S. S. Petrikov
- Subjects
ARDS ,medicine.medical_specialty ,business.industry ,Disease ,030204 cardiovascular system & hematology ,medicine.disease ,medicine.disease_cause ,Sepsis ,03 medical and health sciences ,Pneumonia ,0302 clinical medicine ,Parenteral nutrition ,Community-acquired pneumonia ,Intensive care ,medicine ,030212 general & internal medicine ,business ,Intensive care medicine ,Coronavirus - Abstract
Prophylaxis, diagnostics and correction of nutritional status disturbances is considered as one of the main treatment methods of patients with COVID-19 infection-directed to resolve systemic inflammatory response and correction of metabolic response to a viral infection. Systemic Inflammatory Reaction (SIR) manisfestation as a result of viral infection leads to pronounced metabolic processes disturbances. The main metabolic manifestations of SIR is reflected as hypermetabolic-hypercatabolic syndrome with complex disturbances of protein, lipids and carbohydrates metabolism, increased consumption of carbohydrate-lipid reserves and breakdown of tissue proteins. Thus, adequate correction of metabolic disorders and a wholesome nutritional support, taking into account the clinical picture, severity of the disease, ongoing respiratory and intensive care therapy is an integral component in treating patients with COVID-19 infection which determines the efficiency of its treatment and reduction in mortality. Given the relevance of the problem, the authors decided that it was important to increase the COVID-19 treatment efficacy by producing guidelines based on the most fundamental provisions of the modern approach to nutritional support in critical patients with community acquired pneumonia, acute respiratory failure, ARDS, sepsis, multiple organ failure.
- Published
- 2020
- Full Text
- View/download PDF
38. Changes in energy expenditures while reducing pressure support as a predictor of successful spontaneous breathing trial
- Author
-
Ya. V. Savchenko, A. S. Goryachev, I. A. Savin, K. Yu. Krylov, A. A. Polupan, and T. M. Birg
- Subjects
Mechanical ventilation ,indirect calorimetry ,business.industry ,RC86-88.9 ,medicine.medical_treatment ,respiratory work ,030208 emergency & critical care medicine ,Medical emergencies. Critical care. Intensive care. First aid ,mechanical ventilation ,Critical Care and Intensive Care Medicine ,oxygen consumption ,Spontaneous breathing trial ,03 medical and health sciences ,0302 clinical medicine ,Anesthesiology and Pain Medicine ,030228 respiratory system ,Energy expenditure ,Anesthesia ,energy expenditure ,Emergency Medicine ,Breathing ,weaning from mechanical ventilation ,Medicine ,business ,respiratory comfort - Abstract
The objective: to assess changes in energy expenditures (EE) when reducing pressure support (PS) as a predictor of the patient’s readiness for transfer to spontaneous breathing. Subjects and methods. The study included 33 patients who had been on mechanical ventilation for at least 7 days. PS reduced from 20 to 4 cmH2O at the interval of 4 cmH2O. At each level, the patient's EE were measured with indirect calorimetry. At the end of the study, spontaneous breathing trial (SBT) was performed. Results. In 22 (67%) patients, at least one episode of a statistically significant increase in EE was observed upon transition to a lower level of PS (Group 1). In remaining 11 (33%) patients, with decreased level of PS, EE decreased or did not significantly change (Group 2). SBT was successful in 14 out of 22 patients from Group 1 and all 11 patients from Group 2. The absence of episodes of increased EE during decreased PS was a predictor of successful SBT (sensitivity – 44%, specificity – 100%). Conclusion. The absence of episodes of a statistically significant increase in EE during the transition to a lower level of PS is a highly specific predictor of successful SBT.
- Published
- 2020
39. A generalized action plan for obstetric hospitals and outpatient clinics during the suspected or confirmed COVID-19 pandemic
- Author
-
Nikolai N. Rukhliada, William Kondo, Vitaly A. Reznik, Dmitriy O. Ivanov, Franco Lisi, Sushila Saini, Attila Vereczkey, Stefano Bettocchi, Felice Petraglia, Kirill Yu. Krylov, and Daniel Sanabria S.
- Subjects
Coronavirus disease 2019 (COVID-19) ,business.industry ,viruses ,virus diseases ,General Medicine ,Disease ,medicine.disease_cause ,medicine.disease ,Action plan ,Pandemic ,medicine ,Outpatient clinic ,Medical emergency ,business ,Coronavirus - Abstract
This summary paper presents a generalized action plan for hospitals and outpatient clinics during the COVID-19 coronavirus pandemic. Now the pandemic is the most relevant global challenge. Taking into account that the medical world does not have much clinical experience with COVID-19 and other coronaviruses, such as SARS-CoV and MERS-CoV, thoroughness and caution in assessing and treating pregnant women is an extremely urgent topic. The authors of this article summarized the world experience in managing patients with coronavirus disease and brought it into a unique algorithm that was considered most rational.
- Published
- 2020
- Full Text
- View/download PDF
40. The quality of life of women and morphological changes in the glands of adenomyosis under the influence of dienogest-containing preparations
- Author
-
Nikolai N. Rukhliada, Kirill Yu. Krylov, and Valeriy I Matukhin
- Subjects
Gynecology ,medicine.medical_specialty ,030219 obstetrics & reproductive medicine ,Medical treatment ,business.industry ,Pelvic pain ,Endometriosis ,Myometrium ,medicine.disease ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Dienogest ,chemistry ,030220 oncology & carcinogenesis ,medicine ,Adenomyosis ,medicine.symptom ,business - Abstract
Introduction: “The aim of our study was to analyze the effect of dienogest on the endometrioid glands in myometrium, a morphological assessment of the effect of the drug, taking into account its safety, as well as an assessment of changes in the quality of life of women associated with a decrease in the severity symptoms of adenomyosis.” Materials and methods: The study included women aged 20–50 years, with histologically confirmed adenomyosis, which was manifested in them by the presence of pelvic pain, abnormal uterine bleeding, painful, and/or heavy menstruation, as well as the presence of anemia. Patients were divided into three groups depending on the name of the drug that they used: 24 patients of Group I received the drug “Visanne” at 2 mg/day for 6 months. In the second group, 24 patients received the combined oral contraceptive drug Klaira, which contains gestagen and estrogen. The third group included 24 patients who received the Jeanine preparation continuously (21–21–21) for 6 months and then received it for an additional 6 months according to the scheme (7 + 21), containing ethinyl estradiol and dienogest. Results: After the appointment of drugs for 6–12 months, bloody discharge from the genital tract in Group I completely stopped in all 10 women; in Group II, the discharge decreased in four women and completely stopped in eight women; and in group III, the discharge decreased in three women and completely stopped in seven women, pain in Group I completely stopped in six women out of eight (75%) and decreased in two women out of eight (25%); in the second group, the pain stopped completely in 7 women out of 12 (58.3 %) and decreased in 5 women out of 12 (41.6%); in the third group, pain completely recovered in 6 women out of 10 (60%) and decreased in 4 women out of 10 (40%). According to the morphological assessment (Figures 1 and 2) of the myometrium section with endometrioid glands before treatment, Group I had 12.2 ± 3.8* glands before treatment and from 1.4 ± 0.8* endometrioid glands after treatment. In the second group, the number of glands before treatment was 10.8 ± 1.4* and after treatment from 3.8 ± 0.7 glands, and in the third group before treatment, 6.7 ± 2.1 endometrioid glands were determined and after treatment 2.7 ± 1.4 glands in the field of view (Figure 1). Conclusion: Dienogest is effective in the treatment of endometriosis and reduces both the clinical manifestations of adenomyosis (abnormal uterine bleeding and chronic pelvic pain) and laboratory blood counts, increasing hemoglobin levels, together with a decrease in the activity of the endometrioid glands in myometrium. Dienogest is a highly selective progestogen against progesterone receptors and has anti-proliferative activity in isolated human endometrial cells. Given the indicators of the 36-Item Short Form Survey questionnaire, the quality of life in all the studied groups improved in all parameters, which indicates the effectiveness of the dienogest-containing drugs used.
- Published
- 2020
- Full Text
- View/download PDF
41. Nutritive rehabilitation of the patients after recovering from coma
- Author
-
Alexandra V. Yakovleva, E. M. Mengistu, K. Yu. Krylov, A. E. Shestopalov, and Marina V. Petrova
- Subjects
Coma ,medicine.medical_specialty ,Rehabilitation ,business.industry ,medicine.medical_treatment ,medicine ,In patient ,medicine.symptom ,Prospective cohort study ,Intensive care medicine ,business ,Enteral administration ,Critical condition - Abstract
Background. Along with the measures to restore the basic body functions and prevent secondary brain injury, one of the key areas of modern rehabilitation is nutritional and metabolic support. Aims: to improve the results of the treatment of the patients by optimizing the system of nutritional and metabolic rehabilitation using enteral, parenteral, combined parenteral-enteral nutrition methods, who are undergoing rehabilitation after recovering from Coma resulting from injuries and diseases of the central nervous system. Methods. In a prospective cohort study, the results of the study and complex treatment of 113 patients after recovering from Coma as a result of brain injury of various etiologies who were undergoing rehabilitation in the period from 2017 to 2019 in the FNRC RR were analyzed. Results. Indirect calorimetry has proven to be the most accurate method for creating an effective and adequate program of nutritional and metabolic support in patients in chronic critical conditions who recovers from the coma. In patients who recovers from the coma, due to the excessive process of hypercatabolism, increasing protein requirements: the average protein requirements for a patient who recovers from the coma are 1.261.5 g of protein per kg/weight. Conducting optimal nutritional support for patients recovering from Coma involves determining the functional state of the gastrointestinal tract. To build an effective and adequate program of nutritional and metabolic support where accurate determination of energy needs is of great importance. Conclusions. The indirect calorimetry method is a valuable tool for assessing energy needs, although without taking into account the functionality of the gastrointestinal tract and other factors affecting body compositions, it may not be sufficient to prescribe optimal nutritional support in patients after recovering from Comatose.
- Published
- 2020
- Full Text
- View/download PDF
42. Behcet's disease and associations with HLA-B5 antigen (a review of literature and the authors' findings)
- Author
-
Regina Georgievna Goloeva, Zemfira Sadullaevna Alekberova, Irina Anatol'evna Guseva, Mikhail Yur'evich Krylov, R G Goloyeva, Z S Alekberova, I A Guseva, and M Yu Krylov
- Subjects
behcet's disease ,hla-b5 antigen in behcet's disease ,Medicine - Abstract
Aim. To estimate the distribution of HLA Class I (A, B) antigens in patients with Behcet's disease (BD) and the association of HLA-B5 antigen with the clinical manifestations of the disease in different ethnic and population groups in relation to gender. Subjects and methods. The study covered 93 patients (68 males, 25 females) from the representatives of 24 ethnicities with the verified disease. HLA Class I antigens were typed by the microlymphocytotoxic technique, by applying an antileukocytic serum kit (GISANS, Saint Petersburg). Results. In patients with BD, the prevalence of HLA-B5 antigen proved to be significantly higher than that in the controls (72.0 and 21%, respectively) and to be similar in patients of different ethnicities living in the Caucasus and Transcaucasus (80-83%) while the number of HLA-B5 antigen-positive patients with BD was thrice less in the Russian population than in other BD patients (p < 0.01). There was a significant correlation of HLA-B5 antigen with ocular lesion (retinal angiitis) predominantly in male patients with BD. Conclusion. The prevalence of HLA-B5 antigen was higher in patients with BD than in the population-based control. The diagnostic value of this antigen is not so great, for example, in the Russian population of patients with BD. The presence of HLA-B5 antigen in the phenotype of male patients with BD may be regarded as a prognostically poor marker of development of eye diseases.
- Published
- 2010
43. Allele polymorphism of alkaline, acid soluble phosphatase genes and vitamin D-binding protein in postmenopausal osteoporosis
- Author
-
M Yu Krylov, T A Korotkova, V A Myakotkin, and L I Benevolenskaya
- Subjects
gc genotypes ,association ,osteoporosis ,mineral density of bone tissue ,genetic marker ,Medicine - Abstract
Aim. To study polymorphism of genes involved in mechanisms regulating metabolism of bone tissue: alkaline (ALPL) and acid (ACPI) phosphatases, vitamin D-bindingprotein (GC); to ascertain associations of their genotypes and alleles with osteoporosis (OP) and mineral density of spinal and femoral bone tissue (BTMD). Material and methods. Relevant genetic examination was made in 70 females with OP diagnosed by the WHO criteria (1994) aged 60-79years (mean age 71.0 ± 6.2years) and 51 ОP-free females in the same age interval (mean age 69.0 ± 5.6 years). Polymorphic sites of the genes were examined by polymerase chain reaction. Trinucleotide repeat, ARG105GLN polymorphism of restrictive fragment length (PRFL), [GC, TRH420LYS] PRFL were studied for ALPL gene, ACPI gene and GС gene, respectively. Results. Association was found between frequencies of genotypes SS, 2F and FS, F allele of GC gene with OP as well as between PRFL of the spine, femur and some GC genotypes in OP women. Genes ALPL and ACPI were not associated with OP. Conclusion. It is suggested that genotypes SS, 2F and FS have marked functional differences in fixation and transport of vitamin D active metabolites involved in metabolism of bone tissue in OP.
- Published
- 2004
44. ROLE OF RS7574865 G/T POLYMORPHISMS OF THE STAT4 GENE AND RS2004640 G/T OF THE IRF5 GENE IN PREDISPOSITION TO JUVENILE IDIOPATHIC ARTHRITIS: A PILOT STUDY
- Author
-
Konovalova Nv, I A Guseva, E. Fedorov, S. Salugina, M Yu Krylov, E Yu Samarkina, and D. A. Varlamov
- Subjects
business.industry ,STAT4 gene ,Pediatrics, Perinatology and Child Health ,Immunology ,Medicine ,Arthritis ,Juvenile ,business ,medicine.disease ,Gene ,IRF5 - Published
- 2019
- Full Text
- View/download PDF
45. The rs7574865 polymorphism of the STAT4 gene and risk of early rheumatoid arthritis development (the REMARKA study)
- Author
-
I. A. Guseva, M. Yu. Krylov, N. V. Demidova, A. S. Avdeeva, A. V. Smirnov, E. Yu. Samarkina, E. L. Luchina, D. E. Karateev, and E. L. Nasonov
- Subjects
rheumatoid arthritis ,destructive joint damage ,RC925-935 ,single nucleotide polymorphism ,stat4 gene ,antibodies to cyclic citrullinated peptides ,Diseases of the musculoskeletal system - Abstract
The aim of the study was investigation of association of the rs7574865 polymorphism of STAT4 gene (Signal Transducer and Activator of Transcription 4, a family of signal transduction and transcription activation molecules 4) with very early rheumatoid arthritis (RA) in Russian patients, and the study of the relationship of "phenotype – genotype", particularly of positivity for antibodies to cyclic citrullinated peptides (ACCP), their concentration, the presence of erosive joint damage at inclusion in patients the study with the STAT4 gene polymorphism. Material and methods. The study was conducted in the framework of the program REMARKA (Russian study of Methotrexate and biological drugs in Early active Arthritis). 85 patients with very early RA with a duration of symptoms no more than 6 months, not receiving disease modifying anti-rheumatic drugs (DMARD) and biologicals were included. Results and discussion. The analysis of the distribution of genotypes and alleles of STAT4 rs7574865 polymorphism showed that the frequencies of g/G, G/T and T/T genotypes differ at the level of the prominent tendency to statistical significance between patients with RA and the control group (p=0.05). The frequency of minor allele T in RA is significantly higher than that in the control group, and this allele is associated with a predisposition to RA [odds ratio (OR) 1.7; 95% confidence interval (CI) 1.1–2.8; p=0.03]. In the study of the "genotype – phenotype" relationship, STAT4 gene polymorphism correlated with erosive joint damage (r2=0.289; p=0.008). In carriers of the homozygous genotype TT, the number of erosions at inclusion in the study was significantly higher compared with carriers of genotypes GG/GT (median 5.50 [0.754; 7.5] and 0.00 [0.00; 2.00], respectively; p=0.003). The risk of erosion is also associated with the polymorphism of the STAT4 gene (OR 8.6; 95% CI 1.0–204.9; p=0.03). A difference of the ACCP level depending on STAT4 gene polymorphism was revealed: carriers of at least one minor allele T (G/T+T/T) had significantly higher concentration of ACCP than that in carriers of homozygous GG genotype: 248.97±151.00 and 179.51±147.01 U/ml, respectively; p=0.048).Conclusion. The study showed that in Russian patients with very early RA, the STAT4 gene rs7574865 polymorphism is associated with both predisposition to the disease and prognostically unfavorable manifestations (phenotypes) of the disease, namely, with the development of erosion in the early stages of the disease and an increase of the ACCP level.
- Published
- 2019
46. Vyacheslav Vsevolodovich Ivanov (1929 – 2017)
- Author
-
Vladimir Dybo and Sergey Yu. Krylov
- Subjects
General Earth and Planetary Sciences ,General Environmental Science - Published
- 2019
- Full Text
- View/download PDF
47. Data on distrubution of the phonemes /o/ and /ô/ in the South Russian dialect of the village of Rogovatoye
- Author
-
Aleksandra Ter-Avanesova, Sergey Yu. Krylov, Svetlana Dyachenko, and Anna Malysheva
- Abstract
The paper provides information on the distribution of phonemes /o/ and /ô/ in the South Russian dialect of the village of Rogovatoye (Stary Oskol region, Belgorod district), presenting it in the form of a concise dictionary, which includes data on the reflexes of *o and *ъ in stems, some cases of /o/ and /ô/ that stay for *a, *ě, *e in stems, /o/ and /ô/ in loanwords and a list of inflections with reflexes of *o and *ъ.
- Published
- 2019
- Full Text
- View/download PDF
48. [Intestine morphology and microbiocenosis changes in critically ill patients in neurosurgery]
- Author
-
A. E. Shestopalov, Marina V. Petrova, K. Yu. Krylov, and K.A. Mikaelyan
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Critical Care ,business.industry ,Traumatic brain injury ,Critically ill ,Critical Illness ,Neurosurgery ,030208 emergency & critical care medicine ,medicine.disease ,Wide field ,Neurosurgical Procedures ,Intestines ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Intensive care ,Medicine ,Humans ,Neurology (clinical) ,Microbiome ,business ,Intensive care medicine ,Critical condition - Abstract
In recent years, the effect of critical conditions on intestine and the role of such changes in maintenance and progression of systemic disorders are of particular attention. This issue is relevant in critically ill neurosurgical patients too. Intestine morphology and microbiome changes in these patients represent a wide field for researches in intensive care and prevention of secondary damage to other organs and systems. This review ensures a current approach to the problem of intestine morphology and microbiome changes in critically ill neurosurgical patients. We reviewed the data from clinical studies and experiments reproducing a critical condition in animals. Most publications are indexed in the PubMed, e-library, Google Scholar databases. We also analyzed the data from NEJM, JAMA, Lancet, Critical Care and other issues. The manuscript contains an overview of 44 foreign and 13 domestic references; over 50% of researches were published within the past 5 years. Searching depth was over 50 years.В последние годы все большее внимание уделяется влиянию критического состояния больного на кишечник и роли возникающих в нем изменений в поддержании и нарастании системных нарушений (так называемой энтеропатии критических состояний). Эта проблема актуальна и при критических состояниях у больных нейрохирургического профиля. Вопрос изменения кишечной морфологии и микробиома у таких пациентов представляет собой широкое поле для исследований в области интенсивной терапии и профилактики вторичного поражения других органов и систем. В обзоре представлены результаты систематического поиска публикаций, посвященных изменению морфологии и микробиоценоза кишечника, главным образом у пациентов с нейрохирургической патологией, а также с данными воспроизведения критического состояния в экспериментальных моделях у животных. Большинство публикаций проиндексированы в базах данных PubMed, e-library, Google Scholar, также использованы данные медицинских изданий NEJM, JAMA, Lancet, Critical Care и других. Статья содержит обзор 44 зарубежных и 13 отечественных источников; более половины работ составляют публикации за последние 5 лет. Глубина поиска составила более 50 лет.
- Published
- 2021
49. Relating interferon regulatory factor 5 rs2004640 gene polymorphism to increased risk of systemic sclerosis in the patients: Russian Federation cohort
- Author
-
Lidia P. Ananieva, Mikhail Yu. Krylov, and Irina A. Guseva
- Subjects
030203 arthritis & rheumatology ,0301 basic medicine ,interstitial lung disease ,Medicine (General) ,business.industry ,systemic sclerosis ,Interstitial lung disease ,General Medicine ,interferon ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Increased risk ,R5-920 ,Interferon ,Cohort ,Immunology ,irf5 gene polymorphism ,medicine ,Russian federation ,Gene polymorphism ,business ,medicine.drug ,Interferon regulatory factors - Abstract
Background ― A number of studies confirmed a crucial role of type 1 interferon in pathophysiology of connective tissue diseases. Interferon regulatory factors (IRF) coordinate an expression of type 1 interferon, while interferon regulatory factor 5 (IRF5) gene was recently identified as causing predisposition to systemic lupus erythematosus and Sjögren syndrome. The objective of our study was to identify possible association of IRF5 rs2004640 (G/T) single nucleotide polymorphism with systemic sclerosis (SSc). Material and Methods―The study involved 236 individuals, including 105 patients with SSc diagnosis and 131 control individuals from Moscow region. The latter were healthy, unrelated to each other, their genders and ages were matched to those of SSc patients. Allele-specific real-time polymerase chain reaction (PCR) was used to study IFR5 rs2004640 polymorphism. Results ― We detected significantly higher percentage of IRF5 T-allele carriers in all patients (59.5%), those with diffuse cutaneous SSc (67.3%), patients with interstitial lung lesions (62.3%), and those with positive titers of anti-topoisomerase I antibodies (66.3%), compared with control group (46.2%). The odds ratios (OR) were: 1.71 (р=0.00), 2.40 (р=0.0004), 1.93 (р=0.002), and 2.30 (р=0.0008), correspondingly. Conclusion ― The replacement of nucleotide G by T in the IRF5 rs2004640 gene polymorphism was associated with a predisposition to SSc. Our data implied an existence of a novel SSc pathogenetic pathway associated with important role of type 1 interferon in pathophysiology of connective tissue diseases.
- Published
- 2020
50. Novel coronavirus infection SARS-CoV-2 in elderly and senile patients: prevention, diagnosis and treatment. Expert Position Paper of the Russian Association of Gerontology and Geriatrics
- Author
-
A. Yu. Shchedrina, A. V. Naumov, Yu. V. Kotovskaya, A. V. Rozanov, E. A. Tyukhmenev, F. A. Semenov, I. P. Malaya, Ekaterina N. Dudinskaya, N. O. Khovasova, Irina D. Strazhesko, N. M. Vorobyeva, Olga N. Tkacheva, K. A. Yeruslanova, N. V. Sharashkina, A. S. Milto, L. A. Aleksanyan, T. M. Manevich, E. A. Mkhitaryan, K. Yu. Krylov, N. V. Kotovskaya, and V. S. Ostapenko
- Subjects
Artificial ventilation ,medicine.medical_specialty ,medicine.medical_treatment ,malnutrition ,030204 cardiovascular system & hematology ,medicine.disease_cause ,novel coronavirus infection ,03 medical and health sciences ,Social support ,0302 clinical medicine ,delirium ,medicine ,Diseases of the circulatory (Cardiovascular) system ,030212 general & internal medicine ,Intensive care medicine ,Coronavirus ,senile asthenia ,Geriatrics ,business.industry ,medicine.disease ,Malnutrition ,geriatric assessment ,covid-19 ,Sarcopenia ,RC666-701 ,Position paper ,Delirium ,elderly and senile age ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business - Abstract
A novel coronavirus infection SARS-CoV-2 (COVID19) is especially dangerous for elderly and senile patients. Preventive measures for elderly people should cover three areas: 1) direct prevention of the viral infection, 2) preservation of the functional status and prevention of geriatric syndromes, including the use of social support measures, 3) control of comorbidities. The clinical pattern of COVID-19 in older patients may be atypical, while the mildness of symptoms (no fever, cough, shortness of breath) may not correspond to the severity of the prognosis. Delirium may be the first manifestation of COVID-19, which requires special care in its screening. Management of elderly and senile patients with COVID19 should include measures for delirium prevention, the detection and improvement of nutrition. The risk of malnutrition with sarcopenia increases with hospitalization of a patient, especially when using artificial ventilation, is associated with an unfavorable prognosis during hospitalization, accelerates the progression of senile asthenia and reduces the quality of life. Geriatric assessment is the cornerstone of determining the management of an elderly patient.
- Published
- 2020
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.