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Effective UV protection is a key aspect of substrates directly exposed to UV radiation. Therefore, in the present study, fibrous substrates of core–shell morphology (PCL-core, PVP-shell) containing peptides based on tryptophan, tyrosine and cysteine (W6, YYC2 and YYC3) were prepared. Spectrophotometric studies showed UV absorption by peptides containing tyrosine and tryptophan in the UVB (up to 80%) and UVA (up to 40%) ranges. Cysteine, in turn, contributed to high antioxidant properties, confirmed by DPPH assay. The presence of peptides contributed to a nonwoven fabric characterized by the ability to absorb UV radiation and prevent the occurrence of oxidative stress (caused by the presence of free radicals). In turn, the increase in the surface zeta potential of the nonwoven after UV irradiation and higher thermal stability (demonstrated by DSC studies) indicated the crosslinking of the PVP layer under UVR, which further contributes to the increased protection of the nonwoven against its effects. In summary, obtained nonwoven exhibited functional similarity to the native cornea, providing a potential solution for enhancing corneal tissue engineering and regenerative medicine applications.

Abstract The aim of this study is to investigate whether physical activity and the level of body fat are factors reducing the level of pro‐inflammatory cytokines in people with T1DM. Twenty‐five men (27.8 ± 9.4 years old; 178.9 ± 6.9 cm; 80.6 ± 12 kg) and 18 women (28.1 ± 12.5 years old; 162.4 ± 5.5; 63.1 ± 9.9 kg) were divided into four groups based on body fat percentage and level of physical activity (AN—active people with normal body fat; IAN—inactive people with normal body fat; AO—active people with excessive body fat, IAO—inactive people with excessive body fat). The level of cytokines in the blood serum was assessed. The level of IL‐8 was higher (measurable) in inactive men, regardless of adiposity degree and in women, only in the inactive group with normal body fat. IL‐6 was found only in active men with excessive adiposity. In conclusion, the findings from this study allow to indicate that moderate level of physical activity may contribute to a reduction in the development of systemic low‐grade inflammation in patients with T1DM, and thus, may reduce the risk of CVD.

For several decades, there has been growing interest in the influence of low-frequency magnetic fields (LFMFs) and red LED light on the healing process. Keratinocytes are cells that play a significant role in the process of wound healing and tissue regeneration. A human keratinocyte cell line (HaCaT) was exposed to an LFMF with low induction (180–195 Hz; 60 µT, magnetostimulation), red LED light (630 nm; 300 mW, LED therapy), and their combined action (magneto-LED therapy) in in vitro culture conditions. On day 4 and 8 of the experiment, the following parameters were determined: adhesion/proliferation, adenylate kinase (AK), nitric oxide (NO), cytokines (IL-1β, IL-6, IL-8, IL-10, IL-12p70, TNF-α), metalloproteinases (MMP-2, MMP-9), and collagen IV. It was shown that magnetostimulation caused an increase in keratinocyte adhesion/proliferation and IL-8 secretion and a decrease in IL-12 secretion. The LED therapy resulted in a transient increase in the secretion of NO and cytokines IL-1, IL-12, and IL-6 in keratinocytes. The use of magneto-LED therapy resulted in an increase in keratinocyte adhesion/proliferation, the secretion of cytokines IL-6 and IL-8, and NO with a simultaneous decrease in MMP-9 secretion. The results of our studies showed that the action of an LFMF with low-induction and LED light on keratinocytes can modulate the biological activity of keratinocytes towards improving the skin healing process. [ABSTRACT FROM AUTHOR]

The skin is one of the first organs that manifest disorders of homeostasis occurring in the body. Chronic diseases that manifest themselves as skin problems are often associated with severe stress and the accompanying anxiety, uncertainty and pain, which consequently reduce the quality of patients’ lives. Both stress and dermatoses trigger a cycle of neurohormonal reactions in the body that affects the functioning of the nervous, endocrine and immune systems. The data presented in this article indicate a relationship between stress, the functioning of the immune system and the quality of life of patients with chronic dermatological diseases. The article stresses the necessity of a holistic approach to skin problems in cosmetology, which is a very important element of its care and can decrease stress. Therefore, we believe that care and beauty treatments in combination with aromatherapy and music therapy can complement classical therapy. By reducing stress levels and improving the patient’s health, they can increase the quality of life.

Aim of the study: The purpose of this study was to assess the occurrence of spinal pain syndromes among video game players. Material and methods: 550 persons took part in the survey, including 494 (89.8%) men and 56 (10.2%) women playing video games. The study was conducted using an online questionnaire created using Google Forms. The questionnaire contained 27 questions, including questions about time spent in a sitting position, the occurrence of spinal pain, ways of dealing with pain, and knowledge of the principles of spinal pain prophylaxis. Results: 70% of respondents play on the computer seven days a week, about 3-4 hours a day. Half of the surveyed players devoted one to two hours a day to physical exercise, one-third of the study subjects less than an hour, while every third video game player performed physical activity 3-4 times a week. When pain comes, almost 70% of respondents wait for it to subside, every fourth person treats themselves and the rest seeks help of a doctor or physiotherapist. Conclusions: There was no correlation between the number of years spent on playing video games and the occurrence of spinal pain as well as between the daily number of hours spent on playing video games and the occurrence of spinal pain.

Introduction: Aging facial skin is a natural and unavoidable process. It involves a gradual weakening of the biological activity of cells, extension of regenerative processes and reduction of adaptability. At the same time, a smooth, radiant, wrinkle-free complexion has been a global trend in cosmetology for many years, which results in more and more people, especially women, benefiting from new non-invasive anti-aging treatments. One of such treatments offered on the cosmetology market are treatments utilising RF radio waves. Therefore, the aim of this study was to assess the impact of a series of treatments utilising RF on the skin of the faces of women. Materials and methods: The analysis of the results was carried out by the examined person and by the authors of the project via a point scale of visual assessment of skin condition. The following facial skin parameters were analysed in the study: depth of wrinkles, elasticity, skin brightening and moisturising. Results and conclusions: The conducted own study proves the impact of treatments using RF radio waves on the skin of the face. The applied series of treatments caused a partial reduction of the most visible and deepest wrinkles, as well as improved elasticity, brightening and moisturising of the facial skin.

Emulsion electrospinning is a method of modifying a fibers’ surface and functional properties by encapsulation of the bioactive molecules. In our studies, bovine serum albumin (BSA) played the role of the modifier, and to protect the protein during the electrospinning process, the W/O (water-in-oil) emulsions were prepared, consisting of polymer and micelles formed from BSA and anionic (sodium dodecyl sulfate–S) or nonionic (Tween 80–T) surfactant. It was found that the micelle size distribution was strongly dependent on the nature and the amount of the surfactant, indicating that a higher concentration of the surfactant results in a higher tendency to form smaller micelles (4–9 µm for S and 8–13 µm for T). The appearance of anionic surfactant micelles reduced the diameter of the fiber (100–700 nm) and the wettability of the nonwoven surface (up to 77°) compared to un-modified PCL polymer fibers (100–900 nm and 130°). The use of a non-ionic surfactant resulted in better loading efficiency of micelles with albumin (about 90%), lower wettability of the nonwoven fabric (about 25°) and the formation of larger fibers (100–1100 nm). X-ray photoelectron spectroscopy (XPS) was used to detect the presence of the protein, and UV-Vis spectrophotometry was used to determine the loading efficiency and the nature of the release. The results showed that the location of the micelles influenced the release profiles of the protein, and the materials modified with micelles with the nonionic surfactant showed no burst release. The release kinetics was characteristic of the zero-order release model compared to anionic surfactants. The selected surfactant concentrations did not adversely affect the biological properties of fibrous substrates, such as high viability and low cytotoxicity of RAW macrophages 264.7.

The aim of this study is to investigate whether physical activity and the level of body fat are factors reducing the level of pro‐inflammatory cytokines in people with T1DM. Twenty‐five men (27.8 ± 9.4 years old; 178.9 ± 6.9 cm; 80.6 ± 12 kg) and 18 women (28.1 ± 12.5 years old; 162.4 ± 5.5; 63.1 ± 9.9 kg) were divided into four groups based on body fat percentage and level of physical activity (AN—active people with normal body fat; IAN—inactive people with normal body fat; AO—active people with excessive body fat, IAO—inactive people with excessive body fat). The level of cytokines in the blood serum was assessed. The level of IL‐8 was higher (measurable) in inactive men, regardless of adiposity degree and in women, only in the inactive group with normal body fat. IL‐6 was found only in active men with excessive adiposity. In conclusion, the findings from this study allow to indicate that moderate level of physical activity may contribute to a reduction in the development of systemic low‐grade inflammation in patients with T1DM, and thus, may reduce the risk of CVD. [ABSTRACT FROM AUTHOR]

Background: The available literature lacks studies using the KinesioTaping (KT) method and the hold-relax (HR) technique in working with advanced cancer patients. Patients and methods: The study involved 72 patients (38 women and 34 men), diagnosed with advanced cancer. Patients were randomly assigned either to the KT group (exercise programme and KT), HR group [exercise programme and the HR technique of the proprioceptive neuromuscular facilitation (PNF) method] or C group (a control group, exercise programme). To assess selected parameters the Numerical Rating Scale (NRS) scale and Edmonton Symptom Assessment System — revised (ESAS-r) were used. The physiotherapeutic programme lasted three weeks and took place 5 days a week for 30 minutes within each group. Additionally, in the KT group, kinesio tapes were applied on the paraspinal muscles of the lumbar spine. In the HR group the therapy with the hold-relax technique was applied. Results: There was a statistically significant decrease in pain in all groups and an improvement in the quality of life in patients from both experimental groups. Those changes were significantly greater in the KT group than in both the C group and the HR group. Conclusions: Both KT and HR techniques of the PNF method are effective in reducing pain and improving the quality of life in the examined advanced cancer patients, however, KT has a stronger impact. [ABSTRACT FROM AUTHOR]

Purpose: The aim of this study was to investigate the effect of substrate - polycaprolactone (PCL)-based porous membrane modified with rosmarinic acid (RA), (PCL-RA) and to determine the optimal values of low field laser irradiation (LLLT) as stimulators of biological response of RAW 264.7 macrophages. Methods: The porous polymer membrane was obtained by the phase inversion method, the addition of rosmarinic acid was 1%wt. The reference material was pure polymer membrane. RAW 264.7 were deposited on the material and then irradiated with a laser with a wavelength of 808 nm, a power of 100 mW, an irradiation dose of 2 J/cm²/cell well, applied continuously (C), (100/2/C) or pulsed (I), (100/2/I). Results: Macrophage irradiation resulted in an increase in their adhesion. Modifying the PCL membranes with rosmarinic acid had no effect on cell viability on day 3 of the cell culture. Irradiation of macrophages cultured on PCL-RA material increased their viability. Irradiation of macrophages cultured on PCL-RA material decreased macrophage secretion of NO and protein and the increase in TNF and MCP-1 secretion was only transient on day 3 of culture. Conclusions: Macrophage irradiation had a positive effect on macrophage attachment. Modification of PCL membranes with rosmarinic acid influenced the biological activity of macrophages. Culture of macrophages on rosmarinic acid-modified PCL membranes and simultaneous irradiation of LLLT cells resulted in anti-inflammatory effects. [ABSTRACT FROM AUTHOR]

This work concerns the production of fibrous composite materials based on biodegradable polymers such as alginate, dibutyryl chitin (DBC) and poly-ε-caprolactone (PCL). For the production of fibres from these polymers, various spinning methods were used in order to obtain composite materials of different composition and structure. In the case of alginate fibres containing the nanoadditive tricalcium phosphate (TCP), the traditional method of forming fibres wet from solution was used. However in the case of the other two polymers the electrospinning method was used. Two model systems were tested for biocompatibility. The physicochemical and basic biological tests carried out show that the submicron fibres produced using PCL and DBC have good biocompatibility. The proposed hybrid systems composed of micrometric fibres (zinc and calcium alginates containing TCP) and submicron fibres (DBC and PCL) meet the requirements of regenerative medicine. The biomimetic fibre system, the presence of TCP nanoadditive, and the use of polymers with different resorption times provide a framework with specific properties on which bone cells are able to settle and proliferate.