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Background: People with femoroacetabular with femoroacetabular impingement syndrome (FAIS) often report pain during sports involving repeated sprinting. It remains unclear how sports participation influences running biomechanics in individuals with FAIS. Hypothesis: Changes in running biomechanics and/or isometric hip strength after repeated sprint exercise would be greatest in individuals with FAIS compared with asymptomatic individuals with (CAM) and without cam morphology (Control). Study Design: Controlled laboratory study. Level of Evidence: Level 3. Methods: Three-dimensional hip biomechanics during maximal running (10 m) and hip strength were measured in 49 recreationally active individuals (FAIS = 15; CAM = 16; Control = 18) before and after repeated sprint exercise performed on a nonmotorized treadmill (8-16 × 30 m). Effects of group and time were assessed for biomechanics and strength variables with repeated-measures analyses of variance. Relationships between hip pain (Copenhagen Hip and Groin Outcome Score) and changes in hip moments and strength after repeated sprint exercise were determined using Spearman’s correlation coefficients (ρ). Results: Running speed, hip flexion angles, hip flexion and extension moments, and hip strength in all muscle groups were significantly reduced from pre to post. No significant between-group differences were observed before or after repeated sprint exercise. No significant relationships (ρ = 0.04-0.30) were observed between hip pain and changes in hip moments or strength in the FAIS group. Conclusion: Changes in running biomechanics and strength after repeated sprint exercise did not differ between participants with FAIS and asymptomatic participants with and without cam morphology. Self-reported pain did not appear to influence biomechanics during running or strength after repeated sprint exercise in participants with FAIS. Clinical Relevance: A short bout of repeated sprinting may not elicit changes in running biomechanics in FAIS beyond what occurs in those without symptoms. Longer duration activities or activities requiring greater hip flexion angles may better provoke pathology-related changes in running biomechanics in people with FAIS.

Objectives To quantify the changes in work done by lower limb joint moments during maximal speed running following a sports-specific repeated running protocol. Design Observational with repeated-measures. Methods Recreational athletes (n = 18 (9 females), aged = 26.2 ± 6.2 years) performed 12 maximal 30-m sprints on a non-motorised treadmill. Three-dimensional kinematics and ground reaction forces were subsequently recorded during a 10-m maximal overground sprint before and immediately after the repeated running protocol, from which we calculated work done by sagittal plane hip, knee, and ankle moments. Relative work (J/kg) was reported as a percentage of positive and negative work done by the sum of joint moments. Results Following the repeated running protocol, maximal sprint speed decreased by 19% and was accompanied by reductions in total positive (−1.47 J/kg) and negative (−0.92 J/kg) work, in addition to work done by hip (−0.43 to −0.82 J/kg) and knee (−0.28 J/kg) moments during swing. Compared to before the repeated running protocol, less relative work was done by hip (−9%) and knee (−3%) extension moments during swing. Reductions in work done by hip and knee joint moments during swing were significantly correlated with reductions in maximum running speed (r = 0.61−0.89, p Conclusions A sports-specific repeated running protocol resulted in reductions in mechanical work done by sagittal plane hip and knee joint moments during maximal overground sprinting. Interventions focused on maintaining positive work done by the hip flexors/extensors and negative work done by knee flexors/extensors during the swing phase of running may help prevent reductions in speed following repeated sprinting.

The present work shows the application of nickel- and magnesium-crosslinked gellan microspheres in ionic and affinity capture strategies to directly extract hSCOMT from the complex Komagataella pastoris lysate through a simple batch method. Both formulations present similar morphology, but nickel–crosslinked microspheres present higher crosslinker content and smaller diameters. Four different capture strategies were established, by manipulating the ionic strength, pH, temperature and competing agents' presence. The most promising results for hSCOMT capture and clarification were obtained employing an ionic strategy with nickel-crosslinked microspheres and an affinity strategy with magnesium-crosslinked microspheres at 4 °C. The bioactivity results (200%) and purification degree (70%) of hSCOMT captured by the ionic strategy were more satisfactory probably due to the soft ionic conditions used (100 mM NaCl). For the first time, the gellan polysaccharide versatility was demonstrated in the microsphere application for the direct capture of hSCOMT from a complex lysate, simplifying isolation biotechnological procedures.

Objective: This study determined whether the deep hip muscles could contribute to hip stability. Methods: Hip stability was defined as rotational hip stiffness in the sagittal plane, which was calculated for walking trials for 12 participants via electromyography (EMG)informed neuromusculoskeletal modelling that included all 22 hip spanning muscles. Three model configurations were compared that differed in the excitations of the deep hip muscles, but were identical in the excitations of all other muscles: (1) deep hip muscles informed by intramuscular EMG measurements (assisted activation); (2) deep hip muscles with simulated zero activation (no activation); (3) deep hip muscles with simulated maximal activation (maximal activation). Sagittal plane rotational hip stiffness over the gait cycle was compared between model configurations using a within-participant analysis of variance via statistical parametric mapping (p

The deep hip muscles are often omitted in studies investigating hip contact forces using neuromusculoskeletal modelling methods. However, recent evidence indicates the deep hip muscles have potential to change the direction of hip contact force and could have relevance for hip contact loading estimates. Further, it is not known whether deep hip muscle excitation patterns can be accurately estimated using neuromusculoskeletal modelling or require measurement (through invasive and time-consuming methods) to inform models used to estimate hip contact forces. We calculated hip contact forces during walking, squatting, and squat-jumping for 17 participants using electromyography (EMG)-informed neuromusculoskeletal modelling with (informed) and without (synthesized) intramuscular EMG for the deep hip muscles (piriformis, obturator internus, quadratus femoris). Hip contact force magnitude and direction, calculated as the angle between hip contact force and vector from femoral head to acetabular center, were compared between configurations using a paired t-test deployed through statistical parametric mapping (P 0.05). Additionally, root mean square error, correlation coefficients (R

Background Muscle strength testing is widely used in clinical and athletic populations. Commercially available dynamometers are designed to assess strength in three principal planes (sagittal, transverse, frontal). However, the anatomy of the hip suggests muscles may only be recruited submaximally during tasks performed in these principal planes. Objective To evaluate the inter-session reliability of maximal isometric hip strength in the principal planes and three intermediate planes. Methods Twenty participants (26.1 ± 2.7 years, 50% female) attended two testing sessions 6.2 ± 1.8 days apart. Participants completed 3-5 maximal voluntary isometric contractions for hip abduction, adduction, flexion, extension, and internal and external rotation measured using a fixed uniaxial load cell (custom rig) and commercial dynamometer (Biodex). Three intermediate hip actions were also tested using the custom rig: extension with abduction, extension with external rotation, and extension with both abduction and external rotation. Results Moderate-to-excellent intraclass correlation coefficients were observed for all principal and intermediate muscle actions using the custom rig (0.72–0.95) and the Biodex (0.85–0.95). The minimum detectable change was also similar between devices (custom rig = 11–31%; Biodex = 9–20%). Bland-Altman analysis revealed poor agreement between devices (range between upper and lower limits of agreement = 77–131%). Conclusions Although the custom rig and Biodex showed similar reliability, both devices may lack the sensitivity to detect small changes in hip strength commonly observed following intervention.

Gonçalves, BM, Mesquita, RNO, Tavares, F, Brito, J, Correia, P, Santos, P, and Mil-Homens, P. A new portable device to reliably measure maximal strength and rate of force development of hip adduction and abduction. J Strength Cond Res 36(9): 2465-2471, 2022-Groin injuries are a major issue in sports involving kicking or quick changes of direction. Decreased hip adduction and abduction strength have been indicated as one of the main risk factors for groin injury. The methods currently available to measure hip adduction and abduction strength are reliable but highly dependent on the evaluator skills. Furthermore, several studies have reported the reliability of maximal strength (MVIC), but very few studies investigated the reliability of explosive strength (RFD), a parameter that has been previously shown to have a higher functional value. The aim of the current investigation was to assess the reliability of a user-independent portable dynamometer that concurrently measures MVIC and RFD. Twenty-five healthy young subjects performed maximal isometric hip adduction and abduction in both sitting and supine positions. Measurements occurred in 2 different days separated by 48-72 hours. Test-retest reliability was calculated for both MVIC and RFD. Both MVIC and RFD showed good relative reliability (intraclass correlation coefficient = 0.77-0.98) with no differences between positions or muscle actions. Measurement error was similar between positions for MVIC in both hip adduction and abduction. Measurements of RFD showed higher reliability using a time window of at least 0-100 milliseconds, and lower measurement error was observed in sitting for adduction and in supine for abduction. This study shows that portable dynamometry can be used to concurrently measure hip adduction and abduction maximal and explosive strength, with levels of reliability that are similar to previously described methods.

Objective To compare lower limb strength and countermovement jump (CMJ) kinetics between elite female footballers with and without a history of anterior cruciate ligament reconstruction (ACLR), hamstring strain, or hip/groin injury. Design Cross-sectional. Setting Field-based. Participants 369 elite female Australian football, soccer and rugby league players aged 15–35. Main outcome measures Isometric hip adductor and abductor strength, eccentric knee flexor strength, and CMJ vertical ground reaction forces, including between-leg asymmetry. Players reported their lifetime history of ACLR, and whether they had sustained a hamstring strain, or hip/groin injury in the previous 12-months. Results Players with a unilateral history of ACLR (n = 24) had significant between-leg asymmetry in eccentric knee flexor strength (mean = −6.3%, 95%CI = −8.7 to −3.9%, P < .001), isometric hip abductor strength (mean = −2.5%, 95%CI = −4.3 to −0.7%, P = .008), and CMJ peak landing force (mean = −5.5%, 95%CI = −10.9 to −0.1%, P = .046). Together, between-leg asymmetry in eccentric knee flexor strength, isometric hip abductor strength, and CMJ peak landing force distinguished between players with and without prior ACLR with 93% accuracy. Conclusion Elite female footballers with a history of ACLR, but not hamstring or hip/groin injury, exhibit persistent between-leg asymmetries in lower limb strength and jump kinetics following a return to sport.

Rural and urban environments present significant differences between water and energy consumptions. It is important to know, in detail, which factors related to the consumption of these two resources are different in both environments, once that will be those important to manage and discuss in order to improve its use efficiency and sustainability. This research work involves a survey whose aim is to find the factors that in rural and urban environments may justify the differences found in water and energy consumptions. Besides the collection of water and energy consumption data, this survey analyzed 80 variables (socio-demographic, economic, household characterization, among others), that were chosen among the bibliography as possible factors that should influence water and energy consumptions. After the survey application in rural and urban areas and the data statistical treatment, 42 variables remained as truly differentiating factors of rural and urban environments and so as possible determinants of water and energy consumptions. In order to achieve these objectives, a descriptive data analysis and statistical inference (Mann-Whitney-Wilcoxon test and the Chi-square test of homogeneity) were performed. All the 42 differentiating variables that result from this study may be able to justify these differences, however this will not be presented in the paper and it is reserved for future work., POCI-0-0145-FEDER-016730 Project (PTDC/AAG-REC/4700/2014) with the designation ENERWAT: Water to energy: characterization, modelling and measures for the reduction of urban and rural household consumption, financed by the Foundation for Science and Technology and co-financed by the European Regional Development Fund (FEDER) through the COMPETE 2020 - Programme Operational Competitiveness and Internationalization (POCI). This work was partially supported by the FCT (Portuguese Foundation for Science and Technology) through the project PEst-OE/ECI/UI4082/2013 (C-MADE)

Introduction \ud Hamstring strain injuries typically occur in the proximal biceps femoris long head (BFlh) at high running speeds. Strain magnitude seems to be the primary determinant of strain injury, and may be regulated by muscle activation. In running, BFlh strain is largest in the proximal region, especially at high speeds. However, region-specific activity has not been examined. This study examined the proximal–distal and intermuscular activity of BFlh and semitendinosus (ST) as a function of increasing running speed.\ud \ud Methods \ud Thirteen participants ran at steady speeds of 4.1 (slow), 5.4 (moderate), and 6.8 m·s−1 (fast) on a treadmill. Region- and muscle-specific EMG activity were recorded at each speed using high-density EMG, and were normalized to maximal voluntary isometric activity. Muscle–tendon unit lengths were calculated from kinematic recordings. Speed effects, regional, and intermuscular differences were tested with Statistical Parametric Mapping.\ud \ud Results \ud With increasing running speed, EMG activity increased in all regions of both muscles to a similar extent in the clinically relevant late swing phase. Increases in muscle–tendon unit lengths in late swing as a function of running speed were comparatively small. In fast running, EMG activity was highest in late swing in all regions, and reached 115% ± 20% (proximal region, mean ± 95% confidence limit), 106% ± 11% (middle), and 124% ± 16% (distal) relative to maximal voluntary isometric activity in BFlh. Regional and intermuscular EMG patterns were highly individual, but each individual maintained similar proximal–distal and intermuscular EMG activity patterns across running speeds.\ud \ud Conclusions \ud Running is associated with highly individual hamstring activity patterns, but these patterns are similar across speeds. It may thus be crucial to implement running at submaximal speeds early after hamstring injury for restoration of normal neuromuscular function.

The purpose of this study was to determine the reliability and validity of ultrasonography for measurement of hamstring muscle and semitendinosus (ST) tendon cross-sectional area (CSA). On two consecutive days, muscle anatomical CSA (ACSA) and ST tendon CSA were measured at standardized positions (30%-80% of thigh length; half the distance from the distal muscle-tendon junction to the popliteal crease) on 12 legs using ultrasonography and compared with corresponding magnetic resonance imaging measures. Inter-day intraclass correlation coefficients were good-to-excellent (0.882-0.996) for all assessed muscle and tendon sites. The limits of agreement widths were narrowest (range: 17%-52%) when muscle ACSA was large but were wide at sites with relatively small ACSA (≤184%) and for ST tendon CSA (range: 72%). Results suggest ultrasound-based measures of individual hamstring muscle maximal ACSA are reliable and valid and ST tendon CSA measures are reliable but require comparison with cadaveric or intra-operative measurements to verify validity.

Made available in DSpace on 2019-10-06T16:42:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-11-15 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The present study was developed in a joint partnership with the Brazilian pedometrics community to standardize and evaluate spectra within the 350–2500 nm range of Brazilian soils. The Brazilian Soil Spectral Library (BSSL) began in 1995, creating a protocol to gather soil samples from different locations in Brazil. The BSSL reached 39,284 soil samples from 65 contributors representing 41 institutions from all 26 states. Through the BSSL spectra database, it was possible to estimate important soil attributes, such as clay, sand, soil organic carbon, cation exchange capacity, pH and base saturation, resulting in differences among the multi-scale models taking Brazil (overall), regional and state scale. In general, spectral descriptive and quantitative behavior indicated important relationship with physical, chemical and mineralogical properties. Statistical analyses showed that six basic patterns of spectral signatures represent the Brazilian soils types and that environmental conditions explain the differences in spectra. This study demonstrates that spectroscopy analyses along with the establishment of soil spectral libraries are a powerful technique for providing information on a national and regional levels. We also developed an interactive online platform showing soil sample locations and their contributors. As soil spectroscopy is considered a fast, simple, accurate and nondestructive analytical procedure, its application may be integrated with wet analysis as an alternative to support the sustainable management of soils. Department of Soil Science Luiz de Queiroz College of Agriculture (ESALQ) University of São Paulo (USP), Ave. Pádua Dias 11, Cx. Postal 9 Department of Soil Federal University of Santa Maria, Av. Roraima 1000 Geographical Sciences Department Federal University of Pernambuco, Av. Ac. Hélio Ramos, s/n Department of Agronomy State University of Maringá, Av. Colombo 5790 Department of Agriculture Biodiversity and Forestry Federal University of Santa Catarina, Rodovia Ulysses Gaboardi 3000 - Km 3 Federal Rural University of Amazon, Ave. Presidente Tancredo Neves 2501 Faculty of Agronomy and Veterinary Medicine University of Brasília EMBRAPA - Solos, R. Antônio Falcão, 402, Boa Viagem Center of Nuclear Energy in Agriculture (CENA) USP, Av. Centenário 303 CDRS/Secretary of Agriculture of São Paulo State, R. Campos Salles 507 Department of Soils Federal University of Viçosa, Ave. Peter Henry Rolfs s/n EMBRAPA – Informática Agropecuária, Ave. André Tosello, 209 Department of Nuclear Energy Federal University of Pernambuco, Av. Prof. Luis Freire 1000 Department of Geography Federal University of Rio Grande do Norte, R. Joaquim Gregório s/n Agronomic Institute of Campinas (IAC), Ave. Barão de Itapura 1481 Institute of Agricultural Sciences Federal Rural University of Amazônia, Ave. Presidente Tancredo Neves 2501, 66.077-830 Department of Soil Science Federal University of Lavras Federal University of Mato Grosso, Cuiabá, Av. Fernando Corrêa da Costa 2367 Department of Soils Federal Rural University of Rio de Janeiro, Rodovia BR 465, Km 07 s/n Soil and Water Sciences Department University of Florida, 2181 McCarty Hallr, PO Box 110290 EMBRAPA - Solos, R. Jardim Botânico, 1024 Department of Soils and Fertilizers School of Agricultural and Veterinary Studies São Paulo State University (FCAV-UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n Federal University of Sergipe, Av. Marechal Rondon s/n Graduate Program in Earth Sciences (Geochemistry) Department of Geochemistry Federal Fluminense University, Outeiro São João Batista, s/n Federal Institute of the Southeast of Minas Gerais, R. Monsenhor José Augusto 204 Federal University of Rio Grande do Norte, R. Joaquim Gregório s/n Federal University of Piauí EMBRAPA Milho e Sorgo, Rod MG 424 Km 45 Institute of Agricultural Sciences Federal University of Jequitinhonha e Mucuri Valleys, Ave. Ver. João Narciso 1380 Department of Biosystems Engineering ESALQ USP, Ave. Pádua Dias 11, Cx. Postal 9 Federal University of Acre, Rodovia BR 364 Km 04 Federal University of Amazonas, Av. General Rodrigo O. J. Ramos 1200 EMBRAPA Clima Temperado, BR-392, km 78 Department of Agronomy Federal Rural University of Pernambuco, R. Manuel de Medeiros s/n EMBRAPA Cocais, Quadra 11, Av. São Luís Rei de França 4 Paraense Emílio Goeldi Museum, Av. Gov. Magalhães Barata 376 Exata Laboratory, Rua Silvestre Carvalho Q 11 Federal University of Rondônia, BR 364, Km 9.5 Nacional Institute for Amazonian Research, Ave. André Araújo 2936 Department of Forestry Sciences ESALQ-USP, Ave. Pádua Dias 11, Cx. Postal 9 Department of Soils and Fertilizers School of Agricultural and Veterinary Studies São Paulo State University (FCAV-UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n FAPESP: 2014/22262-0 FAPESP: 2016/26176-6 FAPESP: 2017/03207-6

O experimento teve como objetivo avaliar o tempo que búfalas levaram para voltar ao estado fisiológico normal depois de submetidas a estresse térmico. Foram utilizadas seis búfalas mestiças Murrah com idade aproximada de 2,5 a 3 anos e peso médio de 450 kg. As búfalas foram submetidas a dois tratamentos com três animais cada: banho (B) e sem banho (SB). As variáveis fisiológicas coletadas foram: freqüência respiratória (FR); taxa de sudação (TS) e temperatura retal (TR). Todos os animais ficaram mantidos em piquete de aproximadamente 2000 m², sem disponibilidade de sombra e tanques de água, das 8h00 às 14h00, durante quatro dias não consecutivos de coletas. As coletas foram realizadas antes e após a exposição ao sol, e de hora em hora até as 16h00. A exposição dos animais ao sol fez com que houvesse um aumento significativo nas variáveis fisiológicas, com isso o tratamento banho foi mais eficiente para a variável frequência, respiratória comparado ao tratamento sem banho, para que as búfalas voltassem ao seu estado normal após o estresse térmico.

In this work, a study has been carried out to investigate the resistance of commercial poly(vinylidene fluoride) PVDF in its β phase to UV radiation using a xenon lamp. The radiation was applied for 10 weeks and, during this time, the variation in the chemical structure, crystallinity, dielectric and piezoelectric response was monitored. The results show that PVDF has a very good resistance against the effect of UV radiation as no significant changes were detected either at a molecular level or in the electro-active response. It can be concluded that PVDF shows high stability towards photodegradation and can be used for outdoor applications without loss in performance.

In this work, commercial poly(vinylidene fluoride) PVDF, in its β phase was analysed before and after the samples were exposed to UV radiation using a xenon lamp, for ten weeks. Changes in chemical structures, crystallinity, dielectric and piezoelectric response were investigated. From the present study it can be concluded that PVDF shows high stability towards photodegradation and can be used for outdoor applications without interference in its performance.

Marine compound mooring and towing cables are usually formed by cables, chains, buoys and underwater bodies. They are exposed to the action of the water in relative motion, and hence the need to predict the static configuration of these complex mechanical systems under typical conditions from the early stages of the design process. This paper introduces a new mathematical model and a matching numerical method based on finite differences, in order to predict the static configuration of mooring or towing compound cables. The model is validated by analyzing some case studies using a computer program that was specially written for the purpose. The same program is then used for the analysis of a lazy S riser application. The conclusion is that the new model provides a coherent and efficient means to analyze moored/towed systems.

Poly(vinylidene fluoride), PVDF, in its β-phase is an electroactive polymer with many technological applications. There are two main ways to prepare this polymer in its electroactive β-phase: by high temperature stretching from the α-phase and directly from solution. In this paper, the influence of the processing methods in the thermal stability of the samples was studied by UV–VIS spectroscopy and thermogravimetric analysis. The number of chain defects was measured by 1 H NMR. The results obtained were compared to a commercial β-PVDF sample. The number of head to head defects in the different samples is found to be between 6% and 9%. The onset temperature for thermal degradation and the average activation energy (∼76.5 kJ mol −1 ) of the process are approximately equal for the α-phase sample and the β-phase obtained from it. Larger values of the onset temperature and average activation energy (∼100 kJ mol −1 ) are found for the β-phase sample directly obtained from the solution and for the commercial β-phase sample. The thermal degradation of the samples occurs in two steps, independently of the phase of the sample, the degree of crystallinity and the processing method.

The unfolding of cutinase at pH 4.5 was induced by increasing the temperature and guanidine hydrochloride concentration in the presence of potassium chloride, trehalose, and mannosylglycerate potassium salt. Protein thermal unfolding approached a two-state process, since the unfolding transitions were coincident within experimental error when assessed by near-ultraviolet (UV) difference, tryptophyl, and 8-anilino-1-naphthalene sulfonic acid (ANS) fluorescence spectroscopy. Trehalose at 0.5 M increased the temperature at which 50% of cutinase is unfolded by 3 degrees C. Unfolding induced by guanidine hydrochloride is clearly a non-two-state process. The presence of a stable intermediate was detected because unfolding assessed by near-UV difference spectroscopy occurs earlier than unfolding assessed by tryptophyl fluorescence. The intermediate is molten globule in character: the ANS fluorescence is higher than in the presence of the folded or unfolded state, showing native-like secondary structure and losing many tertiary interactions of the folded state, i.e., those surrounding the tyrosyl microenvironment. The stabilization effect of trehalose and mannosylglycerate was quantified by fitting the unfolding transitions to a model proposed by Staniforth et al. (Biochemistry 1993;32:3842-3851). This model takes into consideration the increase in solvation energies of the amino acid side-chains as the denaturant concentration was increased and the fraction of amino acid side-chains that become exposed in the unfolded structure of cutinase. Trehalose and mannosylglycerate stabilize the folded state relative to the intermediate by 1.4-1.6 and 1.6 kcal/mol and the intermediate relative to the unfolded state by 1.0 and 1.5 kcal/mol, respectively.

The effects of aqueous surfactant solutions on the kinetics and stability of cutinase from Fusarium solani pisi were studied. The surfactant sodium bis[2-ethylhexyl]ester sulfosuccinic acid (AOT) acts as a pseudo-competitive inhibitor within a limited concentration range relative to the hydrolysis of short-chain p-nitrophenyl esters. For higher concentrations a hyperbolic mixed inhibition takes place. A pseudo-activation of hydrolysis in presence of AOT and hexadecyltrimethyl-ammonium bromide (CTAB) was observed. CTAB has similar effects on kinetics of cutinase. Cutinase revealed to be stable in CTAB solutions, with activity retention as high as 80%. AOT has a deleterious effect on the enzyme in the time course, resulting in acute loss of activity possibly related with unfolding of the protein structure. A relation between deactivation rate constants and AOT/cutinase concentration ratios is suggested. The presence of the linear alcohol, 1-hexanol, was included in these solutions, in the attempt to interpret the deactivation of cutinase when encapsulated in reversed micelle systems in the absence of this co-surfactant.

Recombinant cutinase from Fusarium solani pisi was covalently attached to dextran and two derivatized silica supports, Biosil-NH2 and Biosil-Dextran-NH2. Kinetic parameters were determined for all three systems as well as for soluble cutinase. Long-term stability in aqueous media was studied; dextran may have a stabilizing role not only due to the covalent links involved but also in the same way as other polyhydroxides in aqueous media. Differential scanning calorimetry analysis suggests an enhancement of conformational stability of the immobilized forms. http://www.sciencedirect.com/science/article/B6TG1-4165DGC-9/1/b62e6c1431e1ea508958b86755959198

Submitted by Ana Maria Bastos da Costa Segadães (segadaes@ua.pt) on 2012-01-18T16:05:25Z No. of bitstreams: 1 MScF 34-36(1988)705-709.pdf: 1071434 bytes, checksum: ab3489b7010dc3c0631183373563d7d7 (MD5) Made available in DSpace on 2012-01-25T10:34:26Z (GMT). No. of bitstreams: 1 MScF 34-36(1988)705-709.pdf: 1071434 bytes, checksum: ab3489b7010dc3c0631183373563d7d7 (MD5) Previous issue date: 1988 Universidade de Aveiro Instituto Nacional de Investigação Científica

Adsorption isotherms of the pure vapours and of liquid mixtures of water and methanol on charcoal cloth at 293K have been determined. The surface excess isotherms show that methanol is always the preferentially adsorbed component over the whole composition range. The isotherms exhibit an unusual feature with a secondary maximum at high mole fractions of methanol. In order to explain this behaviour charcoal cloth was studied before and after modification of the surface chemical structure. Evidence was found that as the polarity decreases the surface excess increases and the secondary maximum becomes less pronounced.

Aim Physiological race determination of 143 Fusarium oxysporum f. sp. lycopersici (FOL) isolates collected along 30 years in major tomato-producing regions of Brazil. Made available in DSpace on 2021-08-10T02:07:11Z (GMT). No. of bitstreams: 1 jam.14966.pdf: 573920 bytes, checksum: 929ad03882c7bfebc86e1a67aa04999d (MD5) Previous issue date: 2021