1. Human adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine
- Author
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Juan Armendáriz-Borunda, Leonel García-Benavides, Adriana Salazar-Montes, Jesús García-Bañuelos, Juan José Rivera-Valdés, Ana Sandoval-Rodriguez, and Alfredo Dominguez-Rosales
- Subjects
0301 basic medicine ,collagen ,Male ,creatinine blood level ,kidney disease ,kidney fibrosis ,Adipose tissue ,lcsh:Medicine ,transforming growth factor beta1 ,Wistar rat ,chemistry.chemical_compound ,0302 clinical medicine ,Fibrosis ,connective tissue growth factor ,rat ,animal ,genetics ,adipose derived stem cell ,lcsh:Science ,Cells, Cultured ,Kidney ,Multidisciplinary ,messenger RNA ,Stem Cells ,creatinine ,unclassified drug ,adipose tissue ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,creatinine urine level ,chemically induced ,Kidney Diseases ,medicine.medical_specialty ,COL1A1 protein ,animal experiment ,Renal function ,urea ,stem cell transplantation ,Article ,animal tissue ,03 medical and health sciences ,urea blood level ,Internal medicine ,medicine ,gene expression profiling ,Animals ,Humans ,controlled study ,human ,Rats, Wistar ,adenine ,Creatinine ,cell culture ,nonhuman ,business.industry ,animal model ,human cell ,lcsh:R ,fibrosis ,medicine.disease ,Rats ,Transplantation ,CTGF ,stem cell ,030104 developmental biology ,Endocrinology ,chemistry ,7 INGENIERÍA Y TECNOLOGÍA ,ACTA2 protein ,treatment outcome ,cytology ,lcsh:Q ,alpha smooth muscle actin ,business ,Kidney disease - Abstract
Background and aims: ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model. Methods A chronic kidney injury was induced by daily orogastric administration of adenine (100mg/ kg) to male Wistar rats for 28 days. hADSCs were isolated, expanded and characterized before transplantation. hADSC administration was performed in a tail vein at a dose of 2 x106 cells/animal. Animals were sacrificed at 7 days post-treatment. The percentage of fibrotic tissue, serum and urine levels of urea, creatinine, total protein and renal mRNA of COL1A1, TGFB1, CTGF, ACTA2, IL6, IL10, TNF were analyzed. Results hADSCs treatment significantly reduces kidney fibrosis, improves urea and creatinine serum and urine levels, and diminishes COL1A1, TGFB1, CTGF, ACTA2 mRNA kidney levels. Conclusions These results showed that cell therapy using hADSCs improves renal function and reduces fibrosis. © 2017 Rivera-Valdes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Published
- 2017