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Baobab (Adansonia digitata) is a long-lived tree endemic to Africa with economic, ecological, and cultural importance, yet its genomic features are underexplored. Here, we report a chromosome-level reference genome anchored to 42 chromosomes for A. digitata, alongside draft assemblies for a sibling tree, two trees from distinct locations in Africa, and A. za from Madagascar. The baobab genome is uniquely rich in DNA transposons, which make up 33%, while LTR retrotransposons account for 10%. A. digitata experienced whole genome multiplication (WGM) around 30 million years ago (MYA), followed by a second WGM event 3–11 MYA, likely linked to autotetraploidy. Resequencing of 25 trees identify three subpopulations, with gene flow across West Africa distinct from East Africa. Gene enrichment and fixation index (Fst) analyses show baobab retained multiple circadian, flowering, and light-responsive genes, which likely support longevity through the UV RESISTANCE LOCUS 8 (UVR8) pathway. In sum, we provide genomic resources and insights for baobab breeding and conservation. Baobab is a long-lived tree that holds great economic, ecological, and cultural value. Here, the authors report haploid chromosome-level reference genome of Adansonia digitata and draft assemblies for a sibling tree, two trees from distinct locations in Africa, and a related species from Madagascar. [ABSTRACT FROM AUTHOR]

Abstract\nNOVELTY STATEMENT\nSIGNIFICANCE OF THE STUDYThe study investigates the ability of selected tree species to absorb heavy metals (Pb, Ni, Zn) from polluted soils. Seedlings of Adansonia digitata (P1), Jatropha curcas (P2), and Hildegardia barteri (P3) were transplanted into polythene pots with soils from a dumpsite (T1), highway (T2), industrial area (T3), and farmland (T4), forming a 3x4 factorial experiment replicated five times in a Completely Randomized Block Design. Pre-sowing analysis showed T1 and T2 had the highest Pb and Zn concentrations, T3 had the highest Ni, and T4 had the lowest heavy metal concentrations. After 12 weeks, heavy metal concentrations decreased in all soils. P1 concentrated metals in the root, P2 in the shoot, and P3 in various plant parts, with significant differences between species. P2 was identified as an effective phytoextractor for Pb and Zn (TF > 1), and P3 for Ni. All species showed potential for phytostabilization. The study concludes that these species are viable options for phytoremediation of heavy metals in contaminated soils.The study examines the effectiveness of indigenous tree species in phytoremediation, highlighting the importance of using species adapted to local ecosystems for successful land restoration.The study provides essential data for environmental restoration, highlighting sustainable, eco-friendly approaches to soil remediation. These findings have direct implications for policy-making and land management in polluted regions. [ABSTRACT FROM AUTHOR]

Recent studies have used nanoparticle additions to enhance the fuel properties of biodiesel. However, it is unclear how these additives would affect engine operation. The effects of commercial nanoadditives on engines have been the subject of several studies. The current study focuses on nanoparticles derived from agricultural waste – specifically rice husk (RH) – to enhance their value. This study therefore examined silica (SiO2) doped with Adansonia digitata methyl ester (ADME) and tested it in a diesel engine. All nanofuel blends were prepared using an ultrasonication process, incorporating 400 ppm of SiO2 nanoparticles, fuels, and 1% surfactants. The results revealed that the brake thermal efficiencies (BTE) at maximum brake power (BP), for B20, B20 + SiO2, B100, and B100 + SiO2 fuels, were 29.9%, 28.2%, 28.44%, and 27.1%, respectively. Brake‐specific fuel consumption (BSFC) was also reduced when the engine ran from 4 to 16 kW BP. The exhaust gas temperature (EGT) of B100 and B100 + SiO2 increased more than that of B20. The peak heat release rates (HRR) of the B100 + SiO2 and B20 + SiO2 were slightly higher by 2.9% and 2.6%, respectively than the neat B100 at medium BP. However, in‐cylinder gas pressure (CGP) increased in the following order: B20 + SiO2 < B20 < B100 < B100 + SiO2 < B0. Moreover, the exhaust emissions of nanofuel blends showed a greater reduction in CO, total hydrocarbon (THC), CO2, NOX, and particulate matter (PM) in comparison with B20 and B100. Overall, this study recommends that SiO2 nanoadditive is a beneficial substitute fuel additive to use with biodiesel and its blends due to enhanced engine performance efficiency and reduced emissions. [ABSTRACT FROM AUTHOR]

Compared to other drug discovery sources, traditional medicine has significantly contributed to developing innovative therapeutic molecules for preventive and curative medicine. The Baobab tree, also known as Adansonia digitata L., is significant in Africa due to its multitude of benefits and various parts that serve different purposes, providing economic support to rural communities. The analysis of a plant sample using Fourier transform infrared (FT‐IR) spectroscopy detected multiple functional groups, such as carboxyl and aromatic groups. Additionally, gas chromatography‐mass spectroscopy (GC‐MS) was utilized to identify various compounds present in the sample, including tetrachloroethylene and octyl ester. The results of different assays, such as α‐diphenyl‐β‐picrylhydrazyl (DPPH), superoxide, nitric oxide scavenging assays, and total antioxidant by thiobarbituric acid method (TBA) and ferric thiocyanate (FTC) method, demonstrated a substantial scavenging of free radicals and an effective antioxidant efficacy. The bark's antimicrobial activity was tested through agar diffusion, resulting in a range of zone of inhibition from 10.1 ± 0.36 mm to 20.85 ± 0.76 mm. The minimum inhibitory concentration (MIC) value was observed to be approximately 0.625 µg/mL. The biofilm inhibition percentage ranged from 9.89% to 57.92%, with the highest percentage being 57.92%. The GC‐MS and FT‐IR studies revealed phytocompounds, which were then analyzed for their potential therapeutic properties. Computational studies were conducted on the phytocompounds against Pseudomonas aeruginosa and C2 kinase (antioxidant). The study concluded that the Adansonia digitata bark extract and its phytocompound have potential therapeutic efficacy against the target proteins. The best docking scores were about −7.053 kcal/mol and −7.573 kcal/mol for Pseudomonas aeruginosa and C2 kinase (antioxidant), respectively. The interaction patterns with the crucial amino acid residues elucidate the inhibitory efficacy of the phytocompounds. [ABSTRACT FROM AUTHOR]

• The study was commenced to collect and revise data pertaining to the biological impacts of Adansonia digitata. • Our attention was directed towards scholarly articles released from 2015 to January 2024, as documented in the Scopus database. • There is little research conducted on Adansonia digitata plant despite its popular medicinal importance, so we shed light on this plant. Adansonia digitata L. is a highly esteemed and versatile tree native to Africa. It offers numerous benefits in terms of providing medication, food, clothing and serving as a vital source of natural remedies. The increasing demand for natural remedies as opposed to synthetic ones has prompted the exploration of several alternative natural products. The study reviewed research articles published from 2015 to January 2024 and indexed in the Scopus database to provide an up-to-date report on the species' scientific significance. Searches for research papers have been conducted via Elsevier, Springer, Google Scholar, Taylor & Francis, PubMed, and Scopus using " A. digitata ," "chemical composition," "antioxidant," "antibacterial," "antidiabetic," "anticancer," and other pertinent terms. It has been scientifically demonstrated that this plant's fruits, leaves, bark, roots, and seeds all have medicinal potential. This research highlighted the significant potential of the A. digitata tree as a valuable reservoir of phenolic and flavonoid compounds, which can be effectively harnessed for pharmacological purposes. Research undertaken in vitro and in vivo has provided evidence of this plant's helpful role in preventing and treating many ailments. The A. digitata tree exhibits significant potential as an antioxidant and antimicrobial agent as well as an antidiabetic and hepatoprotective agent. A comprehensive evaluation of the compounds identified in the A. digitata tree will yield extremely interesting results. The mechanism of action and clinical assessment should further be determined to ascertain the plant traditional, in vitro, and in vivo animal-documented results. [ABSTRACT FROM AUTHOR]

A few heritage researchers and protection practitioners in some African countries define heritage sites and cultural objects based on European and North American criteria. Such criteria emphasize visual material remains like monumental structures and their aesthetically pleasing features. This Western view overlooks some critical aspects. For example, to local people, the meanings and associated values make the monuments and sites relevant. Despite the inseparability of the tangible heritage and associated intangible aspects such as spiritual practices, some researchers and heritage practitioners, including antiquities department officials, need to pay more attention to the invisible meanings embedded in the sites and cultural objects. Notably, it is these immaterial aspects which the local people value the most. In this article, we survey three fishing villages: Batini, Mkadini and Winde, on the Swahili coast in East Africa, and document what heritage means from the local people's perspective. We argue that fishing communities value features like reef crests, sacred mangrove forests, the ruins of stone-built tombs and sacred baobab trees not because of their physical appearance but the spiritual practices (e.g., healing, rituals, and offerings) the local people undertake within and around these sites. We further contend that to achieve heritage preservation sustainability, government officials and heritage scholars should pay greater attention to the spiritual aspects associated with these places. [ABSTRACT FROM AUTHOR]

Chronic utilization of woody vegetation by African savanna elephants may transform woodland vegetation to open savanna and threaten the extirpation of targeted species. This situation has arisen in Gonarezhou National Park (GNP), Zimbabwe, where a large elephant population (>2 km−2) threatens the persistence of the long‐lived baobab (Adansonia digitata) tree, through elevated mortality levels. This study investigated whether complete or partial refugia from elephant utilization existed for baobabs, and whether protection of trees by management, using wire netting or log‐ or rock‐packs around a tree base, had been effective. A total of 563 baobabs were sampled across northern GNP. Elephants had debarked 99% of trees to some degree, and gouged holes in the stem of 22% of trees. The probability, and severity, of debarking and gouging decreased with increasing distance to permanent water, increasing slope, and the amount of boulder cover. Slope gradient had the greatest effect on elephant impact, with trees located on steep slopes being less affected by elephants than those on flat ground. Distance to water had an effect despite the farthest tree, at 9 km from water, being within the 15 km foraging range of bull elephants. Large boulders partly protected trees even on gentle terrain. None of these factors provided a complete refuge from elephant impact within GNP, but, individually and collectively, provided a partial refuge at current elephant densities. Protection measures doubled tree survival over approximately 5 years, with fencing wire wrapped around baobab stems proving to be the most effective method. Assessment of the future security of baobabs in GNP requires knowledge of their distribution and that of the refugia provided by distance from permanent water, slope, and boulder cover. [ABSTRACT FROM AUTHOR]

Chimpanzees (Pan troglodytes) are categorized as Endangered by the International Union for Conservation of Nature, and habitat loss due to conversion of land for agriculture is one of the major threats to wild populations of this species. This challenging scenario can lead to negative human-chimpanzee interactions, including crop feeding. Chimpanzees consume crops across their geographical range, although little is known about this behavior in savanna habitats. Here we provide new evidence of crop feeding by savanna chimpanzees. We conducted our observations at Dindefelo, a community nature reserve in southeastern Senegal. The chimpanzees were observed to feed on mango (Mangifera indica) and also on baobab (Adansonia digitata), a wild species considered a crop by local people when found in and around villages. Although local people use the fruits of these species for food and income, they tolerated crop-feeding events until recently. In 2023, a case of harassment of a crop-feeding chimpanzee in a mango orchard was witnessed, and four days later a chimpanzee corpse was found at the same place. We conclude that habitat conversion into agricultural fields, uncontrolled bush fires and extraction of wild fruits are the important factors influencing crop-feeding events at Dindefelo. Our findings highlight the need to better understand human-chimpanzee interactions in the anthropogenic landscape of Dindefelo to help mitigate negative attitudes and behaviors towards chimpanzees. [ABSTRACT FROM AUTHOR]

IN MAURITANIA, herbal medicine is an integral part of the culture of the society. However, ethno-medicinal data remain scarce and fragmentary. In this work, the ethno-medicinal knowledge in five regions inhabited mainly by two Mauritanian ethnic groups: the "Maures" and the "Peulhs" was explored. These regions present the climatic stages of the country from hyper-arid to Sahelo-Saharan. This suggests that the use of plants by the population in their health care will depend on their presence in the environment. Our methodology consists of a diagnosis of the current situation of this herbal medicine through an ethno-medical survey of healers in the regions of Tiris Zemmour, Inchiri, Adrar (North), Trarza and Brakna (South West). seventy-six informants were enrolled in this study. Results showed that (93.42%) of the informants belong to the 'Maures' community and that women (64.45%) are more involved in this practice The majority of traditional healers are married (97.38%). The older people and the least educated are the most present in the profession. The ethnobotanical study made it possible to inventory 56 medicinal species, used in phytotherapy, belonging to 25 families. The most cited are Ziziphus lotus, Ziziphus mauritiana, Vachellia tortilis, Adansonia digitata, Gymnosporia senegalensis, and Senegalia senegal. The modes of administration exhibit that the aerial part is the most used (53.50%). The majority of remedies are prepared as infusions (48%) and maceration (22%). The majority of medicines are administered orally (99.77%). Most of medicinal species in the five regions studied are prescribed for diseases of the digestive tract (53.50%). No difference has been observed in the use of this medicine among ethnic groups. these data will serve as a basis for researchers and as a reference for establishing action plans for the conservation of Mauritanian flora in general and traditional medicine in particular. [ABSTRACT FROM AUTHOR]

The metabolic needs of the human body and preventing infections require a diet with sufficient amounts of essential nutrients. This study aimed to investigate the importance of Baobab (Adansonia digitata L.) dried leaves as a healthy food source by determining the content of macro and trace elements in different habitats and regions. This study was conducted in Sudan and covered three different habitats, wetland (W), plainland (P), and mountain (M), in two regions (Blue Nile and Kordofan). The dry matter (DM) of Baobab leaves was considered for analyzed menials, and the results showed that the mean values were significantly affected by habitats where Baobab trees grew. The highest contents of potassium K (1653 ± 34 mg/100 g) and sodium (Na) 7.67 ± 1.18 mg/100 g were found in the W zone, whereas the highest contents of calcium (Ca) 2903 ± 187 mg/100 g and magnesium (Mg) 529 ± 101 mg/100 g were detected in the M and P zones, respectively. In addition, the two regions showed significant differences in trace and macro elements, i.e., higher levels of iron (Fe) 17.17 ± 2.76 mg/100 g and magnesium (556 ± 55 mg/100 g) were found in the Kordofan region while higher levels of zinc (Zn) 2.548 ± 0.55 mg/100 g and calcium (2689 ± 305 mg/100) were in the Blue Nile region. These varying amounts of elements can be used in our daily diets because of their potentially healthy effects, especially in areas where access to nutrient-rich foods is limited. [ABSTRACT FROM AUTHOR]

Baobab (Adansonia digitata L.), seedlings produce a strong prominent taproot, which are known to form root tubers. Baobab domestication is imperative to safeguard its sustainable utilization. Nevertheless, little is known about their growth features and its yield potential. Baobab seedlings develop a tuber-like or taproot which can be consumed as a vegetable. The study aimed to investigate the yield potential of baobab root tubers and number of leaves and the assess variation of fruit types on root development in a nursery trial at five locations in Blue Nile, West and North Kordofan state, Sudan. The collected data included shoot length, stem diameter, number of leaves, taproot length, taproot diameter and root length content. One-way ANOVA showed that that there were significant differences (P≤ 0.05) between studied sites on any of the measured plant growth parameters. Seedlings invested mainly into taproot development during the growth period, with root tubers reaching an average length of 8.13±4.96 cm at 126 days after sowing. Seedlings from El Roseires had longer shoot, tuber, root length, larger number of leaves, stem diameter and tuber diameter. Globose and oblong fruit shape, had variable growth larger than those compared to ovate, obovate, crescent shapes and ellipsoid fruit types. Though, the heterogeneity of root tuber growth by way of affected by abiotic and water availability, as well as genetic origin permit additional study. [ABSTRACT FROM AUTHOR]

The article discloses the AMS (accelerator mass spectrometry) radiocarbon dating results of the well-known African baobab of Dalkut, Dhofar Governorate, Oman. The investigation shows that the baobab has a cluster structure and is composed of 3 perfectly fused stems. Three wood samples were collected from primary branches and one sample was collected from the exterior of a stem. Eight tiny segments were extracted from the samples and dated by radiocarbon. The oldest dated sample segment, which originates from a primary branch of the southern stem, had a radiocarbon date of 590 ± 18 BP, which corresponds to a calibrated age of 685 ± 15 years. This result indicates that the southern stem of the baobab of Dalkut is 800 ± 30 years old. According to other radiocarbon dating results, the two northern stems are younger and emerged from the southern stem around 550 years ago. The tree of Dalkut is a solitary baobab. The nearest baobab is over 150 km away, to the north. [ABSTRACT FROM AUTHOR]

Adansonia digitata L., commonly known as the African baobab, is an important plant within the Malvaceae family. It is a highly nutritious forest tree and holds a special place in several African countries. Despite its prevalence in Africa, its presence is very limited in India, and only a few individual plants of this species are reported from different parts of India, including Kerala. This study focuses on the plants growing at Ajanur and Thalassery in the Kasaragod and Kannur districts of Kerala, respectively. This study reports natural variation in phytochemicals, minerals, and the proximate components of its flowers and leaves at these locations. The results of this study show that total carbohydrate (28.79 %), crude fibre (10.76 %), and protein (53.09 %) content was higher in leaves at Ajanur. In contrast, chlorophyll a, b, and carotenoid content were highest in leaves at Thalassery and showed a value of 39.03 mg/g, 16.65 mg/g, and 13.21 mg/g, respectively. The highest quantity of elements viz. Ca, Zn, K, Fe, Cu, Ni, and Cr were reported in leaves from Thalassery, whereas Mg and Mn were abundant in the leaves from Ajanur. Flowers from Ajanur demonstrated a superior nutritional index, while Thalassery leaves exhibited a higher mineral index. Assessment of antioxidant activity indicated the highest DPPH scavenging activity in the leaves from Ajanur (IC50 = 102.57 μg/mL) and the highest ABTS scavenging activity in its flowers from Thalassery (IC50 = 62.74 μg/mL). The differences in nutritional components and antioxidant properties of the baobab from these two ecological sites underscore the role of ecological factors in its natural variation. Although it is an important forest food tree in the African continent, its potential contribution towards food security from the Indian subcontinent remains untapped.

• Fatty acid content of indigenous seed oils by easy trans-esterification method and GC–MS analysis. • Indigenous South African seed oils are mostly of expected quality and authenticity. • Qualitative and quantitative variation demonstrated for commercially available essential oils. • Industry will benefit from implementation of available SANS standards. South Africa produces a number of indigenous, economically important seed and essential oils. These oils are sold at high prices, which may encourage malpractices such as the adulteration of products, by blending with other less expensive oils, and manipulation of the oil composition of inferior oils by adding specific compounds. Oils produced from wild-harvested plant material, which are typically variable in composition, can influence the batch-to-batch consistency of the oils, both qualitatively and quantitatively. These differences impact on their organoleptic and therapeutic properties. The aim of this study was to investigate the chemical composition of commercially available indigenous fixed oils and essential (volatile) oils, and to compare the levels of characteristic chemical constituents to those reported for authentic oils, to determine the need for compositional guidelines. Selected indigenous seed (n = 24) and essential oils (n = 28), purchased from online suppliers, were chemically characterised using gas chromatography coupled with mass spectrometry and flame ionisation detection. The relative concentrations of four major fatty acids, namely palmitic, stearic, oleic and linoleic acids, varied in the seed oils of Adansonia digitata, Sclerocarya birrea, Citrullus lanatus and Schinziophyton rautanenii. However, the ranges mostly correspond to those reported in literature and to that given in available South African National Standards. One sample each of Adansonia digitata and Citrullus lanatus contained considerably different levels of the four major fatty acids and the cyclopropane fatty acid, dihydrosterculic acid, compared to the other samples in the set. Overall, the main compounds for the five commercial essential oil sample sets were found to vary quantitatively mostly, but were strikingly different in some instances. The chemical profile of one Agathosma betulina sample was found to correspond with that of Agathosma crenulata. Artemisia afra samples contained a compound that may be santolinyl acetate at levels of up to 36.0 %, a compound that was not reported in all authentic oils of the same species. Eriocephalus punctulatus samples contained linalyl acetate at up to 11.9 %, but it was absent from authentic oils investigated in a previous study. Citronellyl acetate and phenyl ethyl alcohol were present in the Pelargonium graveolens samples, but were not reported in a previous study of commercial samples. Lippia javanica samples displayed chemotypic variation, which was also reported in a separate study for the species. A minor degree of qualitative variation was observed in some samples for the essential oils, with compounds absent or present. For the first time, the lack of compositional information that can have a direct impact on consumers is highlighted. As the composition directly impacts on the safety, quality and efficacy of these oils, highlighting the need for producers to follow and provide standardised protocols is suggested. A gas chromatographic profile of seed and essential oils, with semi-quantitative percentage areas and reliable identification of the major constituents present, is recommended as a minimum requirement for quality control. Adherence to this guideline can assist to ensure quality, consistency and authenticity of seed and essential oil products produced and marketed in South Africa. Quality control measures, and where possible, material standardisation, is of paramount importance. The standardisation of preselected plant materials used for essential oil production through cloning and cultivation are key to ensuring chemical consistency and consumer safety. [ABSTRACT FROM AUTHOR]

Background: Baobab fruit is valued for its nutritional and medicinal benefits. Although it is acknowledged that baobab pulp is beneficial for health, studies that link its nutraceutical properties to the ability to eliminate reactive species (ROS and RNS) are scarce. Methods: The nutritional profile and the antioxidant properties of baobab pulp extracts from Angola were evaluated. Thus, for the first time, the evaluation of in vitro scavenging capacity against the most physiologically relevant reactive oxygen species (ROS) and reactive nitrogen species (RNS) were the focus of investigation. Results: Angolan fruit pulp presented high contents of ash (8.0%) and total dietary fiber (52%). Vitamin E content was reported for the first time in fruit pulp. Green solvents were used to quantify bioactive compounds and antioxidant activity. Hydroalcoholic extracts exhibited the highest contents of phenolics (1573.0 mg/100 g) and flavonoids (768.7 mg/100 g). Thus, hydroalcoholic extracts showed higher antioxidant activity, and higher scavenging capacity for ROS (O2•−, H2O2, HOCl, ROO•) and RNS (•NO, ONOO−), being most active for •NO and ONOO−. Conclusion: For the first time, Angolan baobab fruit was described in respect to its nutritional contribution as well as its positive antioxidant effects, both as a functional food and as a nutraceutical ingredient. [ABSTRACT FROM AUTHOR]

Asthma is a chronic respiratory condition which poses a significant global health burden, affecting millions of individuals worldwide. There's growing interest in exploring alternative methods, like medicinal plants, which are crucial for novel drug development opportunities. The aim of the study is to conduct an in-silico investigation on the bioactive compounds present in some medicinal plants traditionally used for treating asthma. The research employed computational software like Maestro 12.8 running on the Windows 10 operating system. Ligand preparation consisted of acquiring SDF format files from the PubChem database and employing the ligprep panel within Maestro 12.8. For protein preparation, protein structures were downloaded from the RCSB directory, and the protein preparation wizard was utilized, followed by receptor grid generation to delineate active sites for docking. Molecular docking procedures were carried out using HTVS, while ADMET predictions were conducted using integrated, in-silico models such as the Swiss ADME online server to evaluate the pharmacokinetic and toxicity properties of the test compounds.A phytoconstituent from Adansonia digitata had higher binding affinity than standard salbutamol against beta-2 receptor, 5 surpassed isobutyl lmethyxanthine (IBMX) against phosphodiesterase (PDE) protein target, while 6 neared fluticasone furoate against glucocorticoid receptor (GR) protein target. 8 phytoconstituents from Allium sativum showed similar affinity to salbutamol against beta-2 receptor, 13 surpassed IBMX against PDE, and 20 approached fluticasone furoate against GR. 10 phytoconstituents from Cynodon dactylon had comparable affinity to salbutamol against beta-2 receptor, 7 to IBMX against PDE, and 7 to fluticasone furoate against GR. 11 phytoconstituents from Casuarina equisetifolia had comparable affinity to salbutamol against beta-2 receptor, 7 surpassed IBMX against PDE, and 12 approached fluticasone furoate against GR. Five phytoconstituents from Euphorbia hirta had similar affinity to salbutamol against beta-2 receptor, 21 surpassed IBMX against PDE, and 11 approached fluticasone furoate against GR, all selected for further screening. ADMET analysis showed favorable pharmacokinetics and toxicity, with post-docking analysis revealing ligand-receptor interactions. These findings underscore the potential of plantderived compounds as candidates for asthma management, warranting further investigation and development. [ABSTRACT FROM AUTHOR]

The removal of pharmaceutically active compounds present in relatively low concentration in wastewater is critical. This is because they have a severe, negative impact on life and the environment. To address this issue, adsorption was used, which is an effective wastewater treatment method for removing substances found in low concentrations in water. This study compared the adsorption performance of active carbon to three biosorbents derived from Adansonia digitata shells. The adsorbents were prepared and characterized using TGA, SEM, EDX, and FTIR analyses and pHPZC. To better understand the adsorption process, equilibrium and reaction kinetics studies were conducted. The effect of contact time, initial phenobarbital concentration, adsorbent mass, and pH was investigated in static conditions. The adsorption results revealed that the biosorbent B3 has a higher affinity for the eliminated compound, with an equilibrium time of 60 min and an adsorption capacity of 47.08 mg/g at an initial concentration of 50 mg/L. The experimental data are consistent with Langmuir and Sips adsorption isotherm models, and with the pseudo-second order and Elovich models for kinetics description. This indicates strong interactions between the adsorbent materials and the pharmaceutical micropollutant. Based on these findings, it appears that, among the tested materials, B3 biosorbent is the most efficient for removing phenobarbital present in low concentrations in water. [ABSTRACT FROM AUTHOR]

This study evaluated the utilization potentials of some endangered savannah tree species in north-west Nigeria. A multistage sampling procedure was employed. Three Local Government areas were selected, from which 36 villages were selected. A structured questionnaire was administered to 360 respondents and ten respondents were randomly selected in each village. Data obtained were analyzed with descriptive statistics. Descriptive statistics were used to assess the collected data. Most (38%) were in the 30- to 39-year-old age range. The majority sex was 100% male, 75% were married and had a family size of at least one to ten people per home. 69% of the respondents have completed formal schooling. (65%) were primarily farmers and (49%) had inherited land. Sixty-five percent of the farms were smaller than one hectare. (40%) had been farmers for an average of 21-31 years. Most farmers (100%) utilized trees as boundary demarcation, 98% as shade and 93% as medicine and wind control. However, 99% utilized trees for medicine. The predominant tree families include Fabaceae, Combretaceae, Rubiaceae, Euphorbiaceae and Caeselpiniaceae. The most familiar tree species were Azadirachta indica, Adansonia digitata, Parkia biglobosa, Lawsonia inermis, Mangifera indica, Vitellaria paradoxa, Tamarindus indica, Jatropha carcus, Diospyros mespiliformis and Acacia nilotica used to treat and cure various ailments and diseases for optimum benefits. [ABSTRACT FROM AUTHOR]

This study explores the isolation and characterization of two acidic polysaccharides from baobab (Adansonia digitata) fruits, named ADPs40-F3 and ADPs60-F3; the two types of acidic polysaccharides exhibited high sugar content and chemical structural features characterized by O–H, C–H, carbonyl C=O, and COOH carboxyl functional groups. The two fractions showed molecular weights of 1.66 × 105 and 9.59 × 104 Da. ADPs40-F3 residues consist of arabinose (2.80%), galactose (0.91%), glucose (3.60%), xylose (34.70%), and galacturonic acid (58.10%). On the other hand, ADPs60-F3 is composed of rhamnose (1.50%), arabinose (5.50%), galactose (2.50%), glucose (3.10%), xylose (26.00%), and galacturonic acid (61.40%). Furthermore, NMR analysis showed that the main acidic structures of ADPs40-F3 and ADPs60-F3 are formed by 4,6)-α-D-GalpA-(1→, →4)-β-D-Xylf-(1→, →4,6)-β-D-Glcp- (1→, →5)-α-L-Araf-(1→, →4,6)-α-D-Galp-(1→ residues and 4)-α-D-GalpA-(1→, →4)-β-D-Xylf-(1→, →6)-β-D-Glcp-(1→, →5)-α-L-Araf-(1→ 4,6)-α-D-Galp-(4,6→, →2)-α-Rhap- residues, respectively, based on the observed signals. Antioxidant assays against DPPH, ABTS+ , and FRAP revealed significant antioxidant activities for ADPs40-F3 and ADPs60-F3, comparable to ascorbic acid (VC). Additionally, both polysaccharides exhibited a dose-dependent inhibition of α-glucosidase and αamylase activities, suggesting potential anti-diabetic properties. In vivo evaluation demonstrated that ADPs60-F3 significantly reduced blood glucose levels, indicating promising therapeutic effects. These findings underscore the potential utility of baobab fruit polysaccharides as natural antioxidants and anti-diabetic agents. [ABSTRACT FROM AUTHOR]

Introduction: The skin is the largest organ of the human body and fulfills protective, immune, and metabolic functions. Skin function and barrier integrity are actively regulated through circadian rhythm--associated genes and epigenetic mechanisms including DNA methylation/demethylation, histone acetylation/deacetylation, and microRNAs. MicroRNA-146a-5p (miR-146a) has been associated with immune activation and skin inflammation; however, the role of miR-146a in regulating skin aging is an open question. This study investigated the role of miR-146a in fibroblasts obtained from different donors in the context of aging, and a potential association of this miRNA with circadian rhythm. Methods: Normal human dermal fibroblasts (NHDFs) from 19y, 27y, 40y, and 62y old donors were used to analyze for miR-146a expression. Expression of miR-146a was downregulated with the hsa-mirVana miR-146a inhibitor, and upregulated with an extract from Adansonia digitata. Effects on markers of skin aging, including cell proliferation, production of Collagen-1 and inflammatory cytokines were assessed. Results: We show that the expression of miR-146a decreases with age in dermal fibroblasts and inhibition of miR-146a in 19y and 62y old NHDFs induced significant changes in essential clock genes indicating an association with circadian rhythm control. Furthermore, downregulation of miR-146a results in a reduction of cellular proliferation, Collagen-1 production, as well as an increase in DNA damage and pro-inflammatory markers. Activation of miR-146a with the Adansonia digitata extract reduced the deleterious effects seen during miR-146a inhibition and increased miR-146a transport through exosome transfer. Conclusion: miR-146a interacts with multiple biological pathways related to skin aging, including circadian rhythm machinery, cell-to-cell communication, cell damage repair, cell proliferation, and collagen production and represents a promising target to fight skin aging. Adansonia digitata extract can promote miR-146a expression and therefore support skin cells' health. [ABSTRACT FROM AUTHOR]

Adansonia digitata L. is a royal tree that is highly valued in Africa for its medicinal and nutritional properties. The objective of this study was to use its fruit shell extract to develop new, powerful mono and bimetallic nanoparticles (NPs) and biochar (BC) using an eco-friendly approach. Silver (Ag), iron oxide (FeO), the bimetallic Ag-FeO NPs, as well as (BC) were fabricated by A. digitata fruit shell extract through a reduction process and biomass pyrolysis, respectively, and their activity against tomato pathogenic fungi Alternaria sp., Sclerotinia sclerotiorum, Fusarium equiseti, and Fusarium venenatum were detected by agar dilution method. The Ag, FeO, Ag-FeONPs, and BC were characterized using a range of powerful analytical techniques such as ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier Transform-Infra Red (FT-IR), dynamic light scatter (DLS), and zeta potential analysis. The fabricated Ag, FeO and Ag-FeO NPs have demonstrated a remarkable level of effectiveness in combating fungal strains. UV-Vis spectra of Ag, FeO, Ag-FeONPs, and BC show broad exhibits peaks at 338, 352, 418, and 480 nm, respectively. The monometallic, bimetallic NPs, and biochar have indicated the presence in various forms mostly in Spherical-shaped. Their size varied from 102.3 to 183.5 nm and the corresponding FTIR spectra suggested that the specific organic functional groups from the plant extract played a significant role in the bio-reduction process. Ag and Ag-FeO NPs exhibited excellent antifungal activity against pathogenic fungi Alternaria sp., S. sclerotiorum, F. equiseti, and F. venenatum. The current study could be a significant achievement in the field of antifungal agents since has the potential to develop new approaches for treating fungal infections. [ABSTRACT FROM AUTHOR]

This work presents some preliminary results on the direct use of untreated biomass from agricultural activities as adsorbents for water treatment. Waste was also used to produce activated carbons (ACs) by chemical activation with KOH. The efficacy of agricultural waste such as stubble, sawdust from Teak (Tectona Grandis), fibers from Imbondeiro (Adansonia digitata L.), bamboo flowers, and other regional plants were tested on methylene blue (MB) removal from the aqueous phase. Adsorption studies were conducted in a batch system and the influence of kinetics, pH, and temperature was evaluated. The adsorption performance of the natural adsorbents was significantly high concerning MB. In particular, Imbondeiro presented a maximum removal capacity of 188.3 mg per gram. This amount was similar to or even higher than the values obtained on ACs produced by their predecessors at 873 K. The studies were finished by constructing slow filters containing natural adsorbents or ACs. The maximum amounts of MB removed on a continuous flux were lower than those obtained for a diversity of untreated biomass types on a batch system. However, these amounts were comparable to the published results obtained on a diversity of untreated biomasses in batch mode. [ABSTRACT FROM AUTHOR]

Rats’ exposure to radiation led to an increased free radical from sub-toxic (24 µg/mol) to toxic concentration (120 µg/mol) in the system of rats. Free radical level exacerbate oxidative stress, thus, leading to hepatic damage, lipid profile and antioxidant alterations.This study investigated the effects of the bioactive constituent of n-butanol subfractions (subf) 1, 2, 3 and 4 of Adansonia digitata (A. digitata) and 1, 2 and 3 of Corchorus olitorius (C. olitorius) leaves on irradiation-induced hepatic damage, lipid profile and antioxidant alterations in system of Rats.A total of 54 rats (198 ± 5.00 g) were distributed into 9 groups of 6 each. Group I were fed with rat chow and distilled water only, all other groups were exposed to a single dose of 6 grey gamma irradiation, such that groups II, III, IV, V, VI, VII, VIII and IX were administered distilled water only, 1000 mg/kg body weight subfractions 1, 2, 3 and 4 of A. digitata, subfraction 1, 2 and 3 of C. olitorius respectively for 7 days.HPLC fingerprinting of the bioactive constituents of the subf of A. digitata and C. olitorius revealed the presence of catechin, eleagnin, quercetin, epicatechin, epigallocatechin, luteolin, astragalin, tocopherol, quercetin and catechin respectively. Irradiation significantly (p

In April 2019, baobab (Adansonia digitata L.) seedlings from Thailand, exhibiting galls on the roots, were intercepted during an import plant quarantine inspection at Chubu Centrair International Airport, Japan. Root-knot nematodes (RKNs) were extracted from the galled roots of baobab seedlings and identified by morphological, morphometrical and molecular methods as the guava root-knot nematode, Meloidogyne enterolobii Yang & Eisenback. The morphology and morphometrics of the intercepted population were similar to those of the original and subsequent descriptions of M. enterolobii. The sequences of D2–D3 of 28S rRNA, mtDNA intergenic COII-16S rRNA and COI genes obtained in this study matched well (99–100% similarity) with each of the gene sequences of M. enterolobii deposited in GenBank. Phylogenetic analysis of these genes revealed that the intercepted population clustered with M. enterolobii and clearly differed from other RKN species. To the best of our knowledge, this is the first report of M. enterolobii from baobab.

Objectives: Epilepsy is a neurological condition characterized by repeated seizures attributable to synchronous neuronal activity in the brain. The study evaluated the effect of acetone extract of Adansonia digitata stem bark (ASBE) on seizure score, cognition, depression, and neurodegeneration as well as the level of Gamma-Aminobutyrate acid (GABA) and glutamate in Pentylenetetrazol-kindled rats. Methods: Thirty-five rats were assigned into five groups (n = 7). Groups 1-2 received normal saline and 35 mg/kg PTZ every other day. Groups 3-4 received 125 mg/kg and 250 mg/kg ASBE orally while group 5 received 5 mg/kg diazepam daily for twenty-six days. Group 3-5 received PTZ every other day, 30 mins after ASBE and diazepam. Results: The results showed that Pentylenetetrazol (PTZ) induces seizure, reduces mobility time in force swim test and decreases the normal cell number in the brain. It also significantly decreases (p < 0.05) catalase, superoxide dismutase and reduced glutathione activities compared to the ASBE pre-treated rats. Pre-treatment with ASBE reportedly decreases seizure activities significantly (p < 0.05) and increases mobility time in the force swim test. ASBE also significantly elevate (p < 0.05) the normal cell number in the hippocampus, temporal lobe, and dentate gyrus. Conclusion: ASBE reduced seizure activity and prevented depression in PTZ-treated rats. It also prevented neurodegeneration by regulating glutamate and GABA levels in the brain as well as preventing lipid peroxidation.

Consumption of wild edible plants (WEPs) is a traditional practice in many rural areas of Sudan and elsewhere in the world. We investigated the traditional uses of WEPs in six villages in Babanosa and Lagawa districts of West Kordofan State, Sudan using guided field walks, direct observations, semi-structured questionnaires and group discussions to collect data on names, characteristics, and uses of WEPs from 150 respondents. We quantified the relative importance of WEPs through use value (UV), fidelity level (FL), and direct matrix ranking (DMR). We recorded a total of 22 WEPs belonging to 21 genera and 15 families. Fabaceae was the dominant family with four genera (19%) and four species (18%), and 11 families contributed one species each. Life forms consisted of shrubs (45.45%), trees (40.9%), herbs (9.09%), and grasses (4.54%). 77% of WEPs exhibited edible fruits that are generally consumed without processing. We recorded the three highest FLs and UVs for Adansonia digitata (94% and 0.87), Balanites aegyptiaca (92.6% and 0.80), and Ziziphus spina-christi (94% and 0.73). Boscia senegalensis was the only species that was consumed as an emergency food to mitigate food shortage. DMR prioritized the medicinal use-category over the other use categories, including food which came in third place. We conclude that domestication of WEPs could secure health, nutrition, and livelihoods for the local population as well as contribute to ecosystem conservation. [ABSTRACT FROM AUTHOR]

Baobab oil is nowadays highly prized by local populations as well as the cosmetic industry. It is a rare oil due to the low oil content of the seeds of the baobab fruit (Adansonia digitata L.). To obtain this oil, the collected seeds were cleaned and ground into powder. Then, the extraction was done by pressing and solvent. The aim of this study is to assess the impact of the extraction method on the composition of baobab oil. According to the results obtained, the solvent extraction process has an influence on the fatty acid composition, especially for oleic (36.42 vs 37.40) % and linoleic (26.85 vs 25.21) % acids. We note a significant difference in the total sterol in favor of the solvent extraction process (370.02 mg.100.g-1 for the oil obtained by pressing against 458.14 mg.100.g-1 for the oil obtained by solvent). Solvent extraction drastically reduces total tocopherols from 1562.4 mg.kg-1 to 547.6 mg.kg-1. The chlorophyll content decreases slightly. However, the carotenoid content increases slightly with solvent extraction. According to the results obtained, the soxhlet solvent extraction process has an effect on the chemical composition of the oil. The heat contributes to the oxidation of the oil and the reduction of sensitive elements such as tocopherols. [ABSTRACT FROM AUTHOR]

The fungal genus Rachicladosporium (Cladosporiales, Cladosporiaceae), typified by cladosporium-like Rachicladosporium luculiae, includes a morphologically diverse assemblage of species. The species of this genus were reported from different substrates, habitats and environments, including plant leaves and needles, twig, black mould on baobab trees, rocks and insects. In this study, four new Rachicladosporium species (R. europaeum, R. ignacyi, R. kajetanii, R. silesianum) isolated from sooty mould communities covering leaves and needles of trees and shrubs in Poland are described. The new species are delineated based on morphological characteristics and molecular phylogenetic analyses using concatenated ITS, LSU, and rpb2 sequences. All newly described species are nested in the main Rachicladosporium lineage (centred around the type species), which contains species that are able to grow at 25 °C. By contrast, four cold adapted, endolithic species known from Antarctica (R. antarcticum, R. aridum, R. mcmurdoi) and Italian Alps (R. monterosanum) form distant phylogenetic lineage and do not grow at this temperature. Therefore, they are accommodated in the new genus Cryoendolithus, typified by Cryoendolithus mcmurdoi. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate nutritional and microbiological properties of beverage made from baobab (adansonia digitata). Specifically, the study evaluated proximate content of beverage made from baobab fruit pulp (Adansonia digitata), assessed mineral composition of beverage made from baobab fruit pulp (Adansonia digitata), determined microbiological properties of beverage made from baobab fruit pulp (Adansonia digitata). A laboratorybased experiment was applied to prepare and analyze samples of the Baobab drink. The results revealed that, even though, the drink had a high moisture content (83.69g) it could supply energy from carbohydrates (12.49g) and contained fat and protein in smaller amounts. Mineral composition was substantial for most nutrients but exceptionally high for potassium (5700ppm). The microbiological properties show that comparatively, the Baobab drink’s microbial status was within safe levels. However, the presence and absence of high moisture in drink and powder influenced the microbial load and isolates from both products respectively. It was recommended that the need to carry out consumer acceptance studies on the drink to guarantee adoption among baobab drinking growing populations. Also a scale-up of this drink to provide healthier replacements for sugar sweetened beverages. [ABSTRACT FROM AUTHOR]

Featured Application: The paper has application potential in the bakery and related industries, considering that the proposed technological solutions can be implemented in the profile units in order to diversify the assortment range of flour products. To provide people with celiac disease with nutrient-rich gluten-free foods, this study aimed to produce cookies based on buckwheat and baobab flours, which were then subjected to nutritional, phytochemical, and sensory analyses. Results demonstrate that baobab flour (BF) and buckwheat flour (BWF) work together to enhance the nutritional properties of the cookies, in that nutrients that BWF is deficient in, BF provides sufficiently, and vice versa. BF is rich in minerals and carbohydrates, while BWF contains comparatively higher fat and protein levels. As for macro- and micro-elements, potassium (K) is the predominant macro-element in BF and BWF, with 13,276.47 ± 174 mg/kg and 1255.35 ± 58.92 mg/kg, respectively. The polyphenol content is higher in BF than BWF, at 629.7 ± 0.35 mg/100 g as opposed to 283.87 ± 0.06 mg/100 g. Similarly, the total flavonoid content and antioxidant activity of BF was greater than that of BWF, while BF exhibited 213.13 ± 0.08 mg/100 g and 86.62 ± 0.04%, in contrast to BWF, which had 125.36 ± 1.12 mg/100 g and 79.72 ± 0.01%, respectively. BF significantly enhanced the phytochemical composition of the cookies, with the richest sample being BBC3 containing 30% baobab. Buckwheat and baobab have the most abundant phenolic compounds of rutin and epicatechin, respectively. About the analysis of sensory attributes of the cookies, the partial substitution of BWF by BF of up to 20% (BWF3) significantly increased the scores for all attributes. Indeed, the appearance (physical aspect of the cookie: whether it is firm or not) and color (influence of baobab addition on cookie coloration) of the cookies were significantly improved with the addition of BF of up to 20%, but above 20% they were less appreciated. Similarly, up to 20% BF, the texture, flavor, and overall acceptability of the cookies were significantly improved. Taste, on the other hand, was not significantly improved, maybe due to the acidic taste provided by the baobab. [ABSTRACT FROM AUTHOR]

Background: It has proven difficult to treat viral infections like SARS-CoV, MERS-CoV, and SARS-CoV-2 that cause severe respiratory disorders with the currently available medications. Alternative strategies for combating viral infections are required to adequately treat infectious diseases like COVID-19. The most potent of them all may be plant-based products or herbal remedies. Following the advent of the COVID-19 epidemic, a number of alternative medicine practitioners in Tanzania developed herbal medicine formulations, claiming that they were effective in treating COVID-19 patients. One among the formulations is Buheri wa Afya, meaning ''good health''. The rationale for choosing Buheri wa Afya over the other formulations was justified by its efficacy in treating COVID-19 patients at different stages of the illness. Purpose: This review aimed to collect information on the chemical composition, biological properties, and ethnomedicinal uses of the constituents of Buheri wa Afya formula, a plant-fungi complex formula made of Adansonia digitata L., Ficus sur Forssk, Securidaca longipedunculata Fresen, Syzygium cumini (L.) Skeels, and Zanthoxylum chalybeum Engl., and a fungus, Ganoderma tsugae Murrill, that was and is still used for the treatment of COVID-19 in Tanzania. Methods: All information regarding ethnomedical applications, chemical compositions, and biological properties related to the constituents of the herbal formula was collected from PubMed, Research4Life, Google Scholar, Science Direct, Web of Science, Wiley Online Library, Springer, Research Gate link, and published books. Results: The search, which mostly focused on COVID-19 and other viral infectious diseases produced a total of 183 publications, with publication dates ranging from 1984 to 2023. Most of the articles originated from African and Asian (specifically China and India) countries. A total of 16 articles spanning between 1984 through 1999 reporting on a variety of topics, such as isolation of compounds and biological testing were collected. During that time, Ganoderma species had the highest number of reported occurrences. In contrast to the 25 papers collected between 2000 and 2009, 78 articles covering the years 2010 through 2019 were collected. These 103 articles reported studies on ethnomedicinal uses, pharmacological properties, and chemical compositions of the plants and mushrooms under review. During the 2020–2023 period, a total 67 different articles were gathered, the major focus being the fight against COVID-19 and other viral infectious diseases. Twelve of them reported the ethnomedical applications of the investigated plant and mushroom species in the Buheri wa Afya formula in the management and treatment of COVID-19 and associated symptoms. The majority of articles reported the presence of a variety of biologically active compounds, including vitamins, flavonoids, phenols, coumarins, alkaloids, terpenoids, macronutrients, and micronutrients. According to the studies, the compounds have been reported to exhibit anti-inflammatory, immunomodulatory, antiviral, antioxidant, antimalarial and many other biological properties. The combination of the observed biological properties of the plant and mushroom species in the Buheri wa Afya formula may be responsible for its effectiveness in the treatment of COVID-19 in Tanzania. Conclusions: The literature has highlighted the indispensable importance of using bBuheri wa Afya as an effective alternative therapy in the management of viral infectious diseases like COVID-19. Various studies reviewed in this study have revealed the potentials of the constituents of Buheri wa Afya in fighting against COVID-19 and other viral infections. Thus, Buheri wa Afya formula with the intrinsic antioxidant, anti-inflammatory, antiviral and immunomodulating properties of its constituents justifies the observed effectiveness of the formula in protection, alleviation of symptoms, and cure of COVID-19 in Tanzania.

This article discusses the discovery of tree-nesting Rüppell's Vultures in Senegal, which is significant because these vultures were previously thought to only nest on rock faces. The breeding distribution of Rüppell's Vultures ranges from Senegal to Tanzania and Ethiopia, and their populations are declining throughout their range. The main threat to these vultures is poisoning, which is also a threat to other vulture species in Africa. The article encourages further surveys in Senegal and adjacent countries to map the size and extent of the regional population. [Extracted from the article]

Baobab (Adansonia digitata L) is a large tree species growing in semiarid and arid lowlands of Ethiopia and other places. The plant is valued by natives for its contributions as a cash crop and livelihood tree. Previous studies using samples from different countries have documented their phytochemical profiles and nutritional and health benefits. This study explored the phytochemical constituents and biological activities of fruit pulp extracts of baobab collected from Tekeze Valley, Tigrai, Ethiopia. To this end, qualitative phytochemical screening tests, quantitative phytochemical analyses, and gas chromatography-mass spectrometry (GC-MS) analysis were carried out using aqueous extract. Analyses of antioxidant activities were also conducted with aqueous- and methanol-extracts using of 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging activity assays. The qualitative screening tests showed the presence of flavonoids, phenols, saponins, tannins, and terpenoids. Quantitative analyses of these phytochemicals at 25, 50, and 100 g/mL aqueous extract resulted in 0.0252 to 0.1000% yields. Yields of flavonoids, phenols, and saponins were higher at 50 g/mL extract, while that of tannins and terpenoids were higher at 100 g/mL. GC-MS analysis resulted in 15 predominant compounds including (1,2bis(trimethylsilyl)benzene (13.17%), 2-methyl-7-phenylindole (11.75%), 2-ethylacridine (10.11%), and benz[b]-1,4-oxazepine-4(5H)-thione,2,3-dihydro-2,8-dimethyl (10.11%). Aqueous and methanol extracts showed concentration-dependent antioxidant activities. In all the assays and concentrations, the antioxidant activities of both extracts were lower than that of the ascorbic acid standard. At equal extract concentrations (e.g., 100 and 250 μg/mL), methanol extract had higher antioxidant activities than aqueous extract. The findings can encourage future initiatives towards large-scale research for compiling a complete phytochemical profile of the fruit pulp of the Ethiopian baobab. [ABSTRACT FROM AUTHOR]

The seeds of baobab were found to have both industrial and domestic uses due to their essential oil qualities for topical medication. However, the seeds found in this study area in Ghana are underutilised and sometimes thrown away after being taken off the pulp. The present study is aimed at examining the impact of the two predominant techniques used for oil extraction from nonoily seeds, namely, mechanical extraction and Soxhlet (n-hexane) extraction, on both the oil yield and physicochemical properties of crude oil derived from baobab seeds. The study looked at the iodine value, peroxide value, acid value, colour, density, and other variables. Refractometers, chroma meters, and titration techniques were used for the determination of specific properties using standard methods. The Soxhlet method of oil extraction was superior in terms of maximum oil recovery, recording a value of 27.75%, in contrast to the mechanical method, which yielded a significantly lower recovery rate of 5.422%. The peroxide and iodine values were found to be 15.09 and 11.89 mEq/g and 85.89 and 88.45 g/100 g for the mechanical and Soxhlet extraction methods, respectively. Statistically significant differences (p ≤ 0.05) were observed between the two oil extraction methods in some of the properties measured. The study discussed the impact of these oil properties on the application of both food and nonfood products. Finally, the study has provided an essential set of data and information to enable product initiators in the cosmetic, food, and other industries to make informed decisions regarding the utilisation of baobab oil as a constituent in the formulation of products. [ABSTRACT FROM AUTHOR]

Adansonia digitata L (Baobab) is a large plant species which thrives in many semiarid regions of the world with remarkable economic and nutritional importance. In Ethiopia, it grows in deserts and hot lowlands. Industrially, baobab fruit pulp (powder) is used for producing good quality jams. This study was carried out to (a) develop a formulation protocol for producing high-quality baobab jam, (b) characterize the jam using standard physicochemical, microbiological, and sensory evaluation methods, and (c) examine the effects of time and temperature of storage on the quality as well as shelf life of the jam. Out of the seven formulations tested, a formulation enriched with 55 g table sugar, 45 g baobab fruit powder, 0.50 g ascorbic acid, and 0.40 to 0.60 g citrus pectin jelling (formulation no. 6) and another one enriched with 60 g table sugar, 40 g baobab fruit powder, 0.50 g ascorbic acid, and 0.40 to 0.60 g citrus pectin jelling (formulation no. 7) resulted in the best jam products. The jam product of formulation no. 7 enriched with 0.50 g ascorbic acid and 0.60 g citrus pectin jelling was better in terms of storage stability and sensory acceptability. Increasing storage time (up to 45 to 90 days) and temperature (from 10–12°C to 25–27°C) lowered the products' nutritional quality and sensory acceptability. High-microbial growths were observed in the products stored at higher temperature longer, but all the microbial loads were far below the acceptable limit. In the absence of measures that improve their shelf lives, household and small-scale jam products have to be consumed fresh or within weeks after their preparation. [ABSTRACT FROM AUTHOR]

The results of a Adansonia digitata provenance and clone trials situated in Sadore (Niger) and Samanko (Mali) indicate genetic variation at both provenance level and among clones. There were significant differences among provenances at Sadore in both survival and height by age 10. Differences in survival were very marked, and suggest that provenances ranged from very poorly- to well-adapted to the Samanko site. However, height was weakly and negatively correlated to survival; possibly the trees may have grown slightly taller because of less competition. West African provenances have a tendency towards faster growth than those of East African origin, and the provenances from drier locations tend to be better adapted. While early variation among 4 year-old clones of leaf development, an important production trait, was indicated, future observations at later ages on fruit traits will be required to make valid selection decisions. The reported 10 year-old provenance variation in tree survival and growth suggest valuable gains could be made by selection of the best provenances. Further trials across a wider range of sites, representative of target planting sites are recommended. Both genebanks contain valuable genetic plant materials worth maintaining for further research and development. [ABSTRACT FROM AUTHOR]

Tree planting success has been a major challenge in the Sahel region. As a low-cost, efficient option, farmer-managed natural regeneration entails some constraints, most notably a lack of species diversity. To find alternative techniques, four experiments were carried out to determine seed germination and seedling emergence capacity, evaluate the success of direct seeding for orthodox (Adansonia digitata, Faidherbia albida, Ziziphus mauritiana) and recalcitrant species (Cordyla pinnata, Saba senegalensis, Vitellaria paradoxa), and examine the effects of fungicide treatments. A fifth experiment studied the influence of planting-hole depth on F. Albida seedling emergence capacity. The results did not reveal any significant differences based on fungicide doses. For germination test, V. paradoxa had the longest mean germination time among recalcitrant species. For both orthodox and recalcitrant species, significant differences were observed in seedling emergence parameters, survival and growth. Eighteen months after sowing, orthodox species A. digitata and Z. mauritiana showed the highest survival rates at 99 ± 1% and 95 ± 2%, respectively, while among recalcitrant species, V. paradoxa had the highest survival rate (80 ± 5%), 17 months after sowing. Analyses of the probability of ending up with a maximum of 10% empty spots demonstrated a need to sow at least 4, 5, 10 and 8 seeds for A. digitata, F. albida, Z. mauritiana, and V. paradoxa, respectively. The deepest hole, measuring 100 cm in the F. albida experiment, significantly outperformed the other holes of lower depth, displaying the highest mean emergence rate and the greatest relative growth rate. These results support the hypothesis that direct seeding in 60 cm-deep holes can be successful for A. digitata, F. albida, Z. mauritiana, and V. paradoxa. [ABSTRACT FROM AUTHOR]

• Controlled mycorrhization and rock phosphate fertilization were two important tools to domesticate indigenous fruit trees with an emphasis on Z. mauritiana. • Controlled mycorrhization and rock phosphate fertilization improved growth and nutrition of indigenous fruit trees in nursery. • Increasing of survival and fruit production of jujube trees in orchards was due to arbuscular mycorrhizal fungal inoculation • Persistence of the introduced arbuscular mycorrhizal fungus with little disturbance in native ones on roots of jujube trees. Ziziphus mauritiana Lam. also named Indian jujube is an important indigenous fruit tree (IFT) to rural people as a source of food and income, whereas it is neglected and underutilized in the West African agroforestry systems. Domestication of Z. mauritiana can help to preserve its genetic resources, to cultivate its germplasm and to improve its fruit production in orchards. Controlled mycorrhization and rock phosphate (RP) fertilization are two approaches to domesticate Ziziphus mauritiana Lam. by improving its growth, nutritional status and fruit production in the P-deficient soils. Here, we review the following (i) relative mycorrhizal dependency (RMD) of Z. mauritiana compared to six Ziziphus spp. [ Z. lotus (L.) Lam., Z. spina-christi (L.) Desf., Z. mucronata Willd., Z. amphibia A. Chev., Z. abyssinica A. Rich. and Z. sphaerocarpa Tul.] and eleven ITFs [ Adansonia digitata L, Aphania senegalensis Radlk, Anacardium occidentale L., Cordyla pinnata (Lepr. ex A. Rich.) Milne-Redhead, Dialium guineensis Wild., Landolphia heudelottii A.DC., Sclerocarya birrea (A. Roch.) Hochst., Saba senegalensis (A. DC.) Pichon, Balanites aegyptiaca (L.) Del., Parkia biglobosa (Jacq.), Tamarindus indica L.], (ii) response to arbuscular mycorrhizal (AM) inoculation and RP fertilization on Z. mauritiana growth and nutrition in nursery, (iii) impact of an introducing exotic AM fungus on already existing AM fungal assemblages in roots of jujube trees after planting, (iv) role of AM fungi and RP fertilizer on growth and fruit production of two Z. mauritiana cultivars (Gola from India and Tasset from Senegal) after planting in arid and semi-arid regions of Senegal. Z. mauritiana provenances and cultivars (Gola and Tasset) appeared to be higher depend on AM fungi than Ziziphus spp. and IFTs. In AM Z. mauritiana seedlings inoculated with Rhizophagus irregularis IR 27, the highest phosphorus dose of RP applied (250 kg P per ha) produced the best plant growth regardless of the sources of the phosphorus applied. The growth-promoting effects of pre-inoculated jujube with the exotic strain R. irregularis IR 27 in nursery was still maintained 18 months after planting, with significant higher rates of survival and an increase of fruit production for inoculated jujube cultivars without disturbance of native AM fungal communities. Inoculated Tasset cultivar gave the highest mean number of fruits (c.a. 300 and per tree), twice the amount of that obtained for Gola, whereas highest mean fresh weight of fruits was obtained for inoculated Gola cultivar (c.a. 670 g per tree). Success and sustained positive effects of pre-inoculation with R. irregularis IR27 hold promise for the role that AM fungal inoculum could play within fruit production of other fruit trees cultivated in West African orchards. [ABSTRACT FROM AUTHOR]

Adansonia digitata L. is an African tree commonly called baobab. This tree is effectively used in traditional medicine to treat cardiovascular disorders. Hyperlipidemia is a well-known cardiovascular risk factor associated with the increased incidence of mortality worldwide. This study aimed to demonstrate the mechanism of baobab polyphenols in the activities of hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and pancreatic lipase as lipid metabolic enzymes. Molecular docking and an incentive for drug design showed that all the polyphenols in baobab bound to the proteins with higher affinity and a lower binding energy compared with simvastatin as the positive control (ΔG: from −5.5 kcal/mol to −6.5 kcal/mol). The same polyphenols exhibited a considerable binding affinity to pancreatic lipase (ΔG: from −7.5 kcal/mol to −9.8 kcal/mol) in comparison with the control and HMG-CoA reductase. Quercetin showed the best docking score from the selected Baobab polyphenols (ΔG = −9.8 kcal/mol). The root mean square deviation (RMSD) results indicated that stable epicatechin and quercetin complexes were demonstrated with HMG-CoA reductase, and other less stable complexes were developed using rutin and chlorogenic acid. Moreover, the analysis of the root mean square fluctuation (RMSF) simulation results was consistent with that of the RMSD. The RMSF value for all the baobab polyphenols, including the crystal control ligand, was kept between 0.80 and 8.00 Å, similarly to simvastatin, and less than 4.8 Å for pancreatic lipase. Chlorogenic acid, quercetin, epicatechin, and rutin had negative ΔG binding scores from highest to lowest. The same ligands displayed more negative ΔG binding scores than those observed in HMG-CoA reductase and crystal control ligand (methoxyundecyl phosphinic acid) in their simulation with pancreatic lipase. In conclusion, baobab polyphenols interact with HMG-CoA reductase and pancreatic lipase to inhibit their substrate binding and block their activity. [ABSTRACT FROM AUTHOR]

The aim of this paper is to improve the nutritional quality of bakery products by replacing wheat flour (WF) with different proportions (10%, 20%, and 30%) of baobab flour (BF). The composite flours and bread obtained were evaluated from nutritional, physical-chemical, phytochemical, organoleptic, and rheological points of view. The results obtained show that BF is a rich source of minerals (K: 13,276.47 ± 174 mg/kg; Ca: 1570.67 ± 29.67 mg/kg; Mg: 1066.73 ± 9.97 mg/kg; Fe: 155.14 ± 2.95 mg/kg; Na: 143.19 ± 5.22 mg/kg; and Zn: 14.90 ± 0.01 mg/kg), lipids (1.56 ± 0.02 mg/100 g), and carbohydrates (76.34 ± 0. 06 mg/100 g) as well as for the phytochemical profile. In this regard, the maximum contents for the total polyphenols content (TPC) were recorded in the case of bread with 30% BF (297.63 ± 1.75 mg GAE/100 g), a total flavonoids content (TFC) of 208.06 ± 0.002 mg QE/100 g, and 66.72 ± 0.07% for antioxidant activity (AA). Regarding the physical-chemical, rheological, and organoleptic analysis, the bread sample with 10% BF (BWB1) was the best among the samples with different proportions of BF. It presented a smooth, porous appearance (73.50 ± 0.67% porosity) and an elastic core (85 ± 0.27% elasticity) with a volume of 155.04 ± 0.95 cm3/100 g. It had better water absorption (76.7%) than WF (55.8%), a stability of 5.82 min, and a zero-gluten index. The scores obtained by BWB1 for the organoleptic test were as follows: Appearance: 4.81; color: 4.85; texture: 4.78; taste: 4.56; flavor: 4.37; and overall acceptability: 4.7. This study shows that BF improved the nutritional quality of the product, organoleptic properties, α-amylase activity, viscosity, and phytochemical profile, resulting in composite flour suitable for the production of functional bread. [ABSTRACT FROM AUTHOR]

Yoghurt was produced from fresh cow milk inoculated with baobab pulp powder. Baobab pulp powder was added at 0.34%, 0.52%, 0.69%, and 0.85% respectively, while the control sample (A) was yoghurt produced from fresh cow milk inoculated with regular commercial starter culture. The physical, chemical, microbiological, anti-nutritional and sensory properties of the samples were analysed using standard procedures. The moisture content, crude protein and fat content decreased with the added baobab pulp powder, while the fibre, ash and carbohydrate contents increased. The pH ranged from 4.34 to 4.74, coupled with negligible anti-nutritional composition. The titratable acidity increased with added baobab pulp powder from 0.52-0.69%. Brix and viscosity of treated samples increased respectively from 30.85-40.02 and 200.05-200.14. Total bacterial and fungi counts ranged from 8.65×104 to 15.51×104 Cfu/mL and 1.12×104 to 4.54×104 Cfu/mL respectively, with the control sample having the higher loads. The over-all acceptability of the samples were significant (p>0.05); sample E (0.85% inoculation) was the most preferred, followed by sample B (0.34% inoculation), while the least accepted sample was the control. Inoculating milk with baobab pulp powder produced yoghurt with improved and acceptable qualities. [ABSTRACT FROM AUTHOR]