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It is of significant importance to public health that reliable monitoring of nitroimidazoles be conducted, while certified reference materials (CRMs) are essential for accurate and reliable detection. A project has been initiated with the objective of developing nitroimidazole purity CRMs to ensure that results from nationwide monitoring laboratories for nitroimidazoles in antibiotic residues can be compared and traced. The candidates were successively characterized in terms of their structure by means of infrared (IR) spectroscopy and mass spectrometry (MS). The mass balance (MB) method and the quantitative nuclear magnetic resonance (qNMR) method were utilized to determine the purity of nitroimidazoles with remarkable accuracy. Furthermore, a methodical investigation was conducted on homogeneity, stability, and uncertainty. Six nitroimidazole purity CRMs, including tinidazole (GBW09252), secnidazole (GBW09286), ronidazole (GBW09288), metronidazole (GBW(E)090755), dimetridazole (GBW(E)090819), and ornidazole (GBW(E)090820), were finally manufactured following authorization from China's State Administration for Market Regulation (SAMR). By using these CRMs, it is possible to improve the traceability, accuracy, and comparability of nitroimidazole measurements in a range of agricultural products, protecting public health. [ABSTRACT FROM AUTHOR]

Introduction: Invasive mechanical ventilation (IMV) is frequently used as a life-supporting device in Pediatric Intensive Care Units (PICU). To date, there are few studies evaluating the impact of prolonged mechanical ventilation (PMV) in children which is associated with high morbidity and mortality. We aimed to determine the incidence and factors associated with PMV in PICU at our institution. Methods: A retrospective review was performed of children aged 1 month to 18 years who were admitted to the PICU at Chiang Mai University Hospital, Thailand between January and December 2020. PMV was defined if the duration of IMV was ≥ 96 hours. Baseline characteristics and factors associated with PMV were analyzed by descriptive statistics, and univariable and multivariable logistic regression analysis, respectively. A p-value of < 0.05 was considered significant. Results: Ninety-two episodes of IMV were performed in 90 children. The median (IQR) age of the children was 22.8 (7.2–111.9) months (male 64.1%). Forty-six of 92 (50%) children received PMV and 32.6% of children with PMV required a tracheostomy. Following multivariable analysis, factors associated with PMV were age <2 years old (OR 2.86, 95% CI 1.04–7.84, p = 0.041), male gender (OR 3.21, 95% CI 1.15–8.94, p = 0.026), and multiple antibiotics administration during PICU admission (OR 7.83, 95% CI 1.87–32.78, p = 0.005), respectively. Conclusions: Pediatric PMV was notably common, with younger age, male gender, and multiple antibiotic use contributing to higher risk. Developing weaning protocols and strategies to reduce PMV duration is crucial. [ABSTRACT FROM AUTHOR]

Graphitic carbon nitride nanorods/titanium dioxide microspheres (g-C 3 N 4 NRs/TiO 2 MSs) Z-scheme heterojunctions were simply prepared by a one-step hydrothermal method, which showed improved visible-light-induced photocatalytic activity for the degradation of tetracycline hydrochloride (TC-HCl). The photodegradation activity of g-C 3 N 4 NRs/TiO 2 MSs is 6.6 and 1.9 times better than that of pristine g-C 3 N 4 and TiO 2 , which is assigned to the unique morphology and formed heterojunction. Hydroxyl radical (⋅OH) and superoxide radical (⋅O 2 −) were the main species for TC-HCl photodegradation according to capture experiments, and a possible mechanism is proposed. This study provides an effective reference for the preparation of binary heterostructures with excellent photocatalytic activity from a simplified process. Z-scheme g-C 3 N 4 NRs/TiO 2 MSs heterojunction was successfully constructed from the simple one-step process which shows enhanced photocatalytic activity and good stability for treating refractory tetracycline hydrochloride. The enhancement is assigned to unique morphology and the formed heterojunction which facilitates charge transfer and visible light absorption. [Display omitted] • Z-scheme g-C 3 N 4 NRs/TiO 2 MSs heterojunction was simply constructed by a one-step hydrothermal method. • TiO 2 microspheres were tightly attached to the surface of g-C 3 N 4 nanorods. • g-C 3 N 4 NRs/TiO 2 MSs (0.8:1) shows 6.6-fold higher TC-HCl degradation rate than g-C 3 N 4 NRs. • The improved activity is related to the unique morphology and heterojunction. [ABSTRACT FROM AUTHOR]

The application of antibiotics has advanced modern medicine significantly. However, the abuse and discharge of antibiotics have led to substantial antibiotic residues in water, posing great harm to natural organisms and humans. To address the problem of antibiotic degradation, this study developed a novel catalytic membrane by depositing Co catalysts onto MXene nanosheets and fabricating the polyethersulfone composite (Co@MXene/PES) using vacuum-assisted self-assembly. The dual role of MXene as both a carrier for Co atoms and an enhancer of interlayer spacing led to improved flux and catalytic degradation capabilities of the membrane. Experimental results confirmed that the Co@MXene/PES membrane effectively degraded antibiotics through peroxymonosulfate activation, achieving up to 95.51% degradation at a cobalt concentration of 0.01 mg/mL. The membrane demonstrated excellent antibacterial properties, minimal flux loss after repeated use, and robust anti-fouling performance, making it a promising solution for efficient antibiotic removal and stable water treatment. [ABSTRACT FROM AUTHOR]

In this paper, we report a simple yet efficient method for rapid and high-performance SERS substrate fabrication by a combination of cold plasma and laser treatment. Our analysis reveals that cold plasma pre-treatment significantly reduced surface roughness, transforming 200 nm spikes into an almost perfectly uniform surface, while enhancing the substrate's surface energy by lowering the water contact angle from 59° to 0°, all achieved within just 30 s of 0.9-mW plasma treatment, while 15-min green-laser treatment facilitated more uniform deposition of AuNPs across the entire treated area, effectively creating the SERS substrates. The combined treatments result in enhancement of the Raman intensity (11 times) and consistency over the whole area of the SERS substrates, and their reusability (up to 10 times). The fabricated SERS substrates exhibit a significant enhancement factor of approximately 3 × 10⁸ with R6G, allowing detection down to a concentration of 10−12 M. We demonstrate the application of these SERS substrates by detecting amoxicillin—an antibiotic used worldwide to treat a diversity of bacterial infections—in a dynamic expanded linear range of seven orders (from 10−3 to 10−9 M) with high reliability (R2 = 0.98), and a detection limit of 9 × 10−10 M. Our approach to high-performance SERS substrate fabrication holds potential for further expansion to other metallic NPs like Ag, or magnetic NPs (Fe3O4). [ABSTRACT FROM AUTHOR]

Smartphone-derived colorimetric tools have the potential to revolutionize food safety control by enabling citizens to carry out monitoring assays. To realize this, it is of paramount significance to recognize recent study efforts and figure out important technology gaps in terms of food security. Driven by international connectivity and the extensive distribution of smartphones, along with their built-in probes and powerful computing abilities, smartphone-based sensors have shown enormous potential as cost-effective and portable diagnostic scaffolds for point-of-need tests. Meantime, the colorimetric technique is of particular notice because of its benefits of rapidity, simplicity, and high universality. In this study, we tried to outline various colorimetric platforms using smartphone technology, elucidate their principles, and explore their applications in detecting target analytes (pesticide residues, antibiotic residues, metal ions, pathogenic bacteria, toxins, and mycotoxins) considering their sensitivity and multiplexing capability. Challenges and desired future perspectives for cost-effective, accurate, reliable, and multi-functions smartphone-based colorimetric tools have also been debated. [ABSTRACT FROM AUTHOR]

In China, antibiotic mycelial residue is categorized as hazardous waste. To achieve the harmless and resourceful disposal of cephalosporin, three types of biochars from cephalosporin mycelia residues, namely non-activated carbon (BC1), ZnCl2-activated carbon (BC2), and KOH-activated carbon (BC3), were respectively fabricated by high-temperature pyrolysis carbonization technology. These three kinds of biochars were characterized via iodine value, FTIR, and SEM, and the adsorption performance of the prepared biochars was investigated using cefuroxime (CXM) as the adsorption target. The results indicated that BC3 biochar possesses the most well-developed pores and the highest iodine value of 1367.41 mg/g; The most suitable dosage is 1.6 g/L, and the lower the pH, the more favorable the adsorption effect. The investigation of adsorption kinetics revealed that it conformed to the kinetic model of pseudo-second order, as well as the process of adsorption was governed by the chemical adsorption mechanism, the rate of adsorption was affected by the collective impact of the quantity of active sites present and the interaction strength between the CXM molecules and the biochar. The exploration of adsorption thermodynamics revealed that it aligned with the Langmuir model, the surface of biochar was relatively uniform, and the adsorption was mainly of low coverage; The calculation of thermodynamic parameters demonstrated that the adsorption was exothermic and spontaneous. [ABSTRACT FROM AUTHOR]

Residues of β-lactam antibiotics (β-LA) in the environment have posed a great threat to human health, while lacking a simple, effective, and universal sensing method. Herein, basic fuchsin and graphdiyne (GDY) were used as precursors to prepare the first reported acidity/basicity-sensitive GDY quantum dots (S-GDY QDs). We propose a novel fluorescence-sensing strategy for β-LA detection based on the ability of β-lactamases to catalyze β-LA to form carboxylic acid, which further induces a change in the acidity/basicity of the solution and causes a decrease in the fluorescence intensity of S-GDY QDs. Furthermore, a fluorescence test strip sensing platform integrated with a smartphone was established to achieve rapid, portable, and visual monitoring of β-LA. Using penicillin G as a model, a detection limit as low as 15.7 nM was achieved, showing important implications for β-LA detection. [ABSTRACT FROM AUTHOR]

A photocatalytic electrochemical aptasensor for the detection of chloramphenicol（CAP）antibiotic residues in water by utilizing SYBR Green I（SG）and chemically exfoliated MoS 2 （ce-MoS 2 ） as synergistically signal-amplification platforms. [Display omitted] • A novel photocatalytic electrochemical aptasensor was developed for the detection of chloramphenicol (CAP) antibiotic residues in water. • Photosensitized SYBR Green I (SG) and chemically stripped MoS 2 (ce-MoS 2) were intercalated into the groove of the resulting dsDNA and use as a synergistic signal-amplification platform. • Upon light irradiation, the photocatalytic reaction was activated, generating singlet oxygen that efficiently cleaved the dsDNA, leading to a significant increase in the current of [Fe(CN) 6 ]3-/4-. • The aptasensor demonstrated good selectivity towards CAP in the presence of interfering antibiotics. • The aptasensor exhibited a linear relationship between CAP and logarithmic concentrations from 20 to 1000 nM, with a detection limit (3σ) of 3.391 nM. This study reports the development of a photocatalytic electrochemical aptasensor for the purpose of detecting chloramphenicol (CAP) antibiotic residues in water by utilizing SYBR Green I (SG) and chemically exfoliated MoS 2 (ce-MoS 2) as synergistically signal-amplification platforms. The Au nanoparticles (AuNPs) were electrodeposited onto the surface of an indium tin oxide (ITO) electrode. After that, the thiolate-modified cDNA, also known as capture DNA, was combined with the aptamer. Subsequently, photosensitized SG molecules and ce-MoS 2 nanomaterial were inserted into the groove of the resultant double-stranded DNA (dsDNA). The activation of the photocatalytic process upon exposure to light resulted in the generation of singlet oxygen. The singlet oxygen effectively split the dsDNA, resulting in significant enhancement in the current of [Fe(CN) 6 ]3-/4-. When the CAP was present, both SG molecules and ce-MoS 2 broke away from the dsDNA, which turned off the photosensitization response, leading to significant reduction in the current of [Fe(CN) 6 ]3-/4-. Under the optimal conditions, the aptasensor exhibited a linear relationship between the current of [Fe(CN) 6 ]3-/4- with logarithmic concentrations of CAP from 20 to 1000 nM, with a detection of limit (3 σ) of 3.391 nM. The aptasensor also demonstrated good selectivity towards CAP in the presence of interfering antibiotics, such as tetracycline, streptomycin, levofloxacin, ciprofloxacin, and sulfadimethoxine. Additionally, the results obtained from the analysis of natural water samples using the proposed aptasensor were consistent with the findings acquired through the use of a liquid chromatograph-mass spectrometer. Therefore, with its simplicity and high selectivity, this aptasensor can potentially detect alternative antibiotics in environmental water samples by replacing the aptamers based on photosensitization. [ABSTRACT FROM AUTHOR]

Motivation Antibiotic resistance has emerged as a major global health threat, with an increasing number of bacterial infections becoming difficult to treat. Predicting the underlying resistance mechanisms of antibiotic resistance genes (ARGs) is crucial for understanding and combating this problem. However, existing methods struggle to accurately predict resistance mechanisms for ARGs with low similarity to known sequences and lack sufficient interpretability of the prediction models. Results In this study, we present a novel approach for predicting ARG resistance mechanisms using ProteinBERT, a protein language model (pLM) based on deep learning. Our method outperforms state-of-the-art techniques on diverse ARG datasets, including those with low homology to the training data, highlighting its potential for predicting the resistance mechanisms of unknown ARGs. Attention analysis of the model reveals that it considers biologically relevant features, such as conserved amino acid residues and antibiotic target binding sites, when making predictions. These findings provide valuable insights into the molecular basis of antibiotic resistance and demonstrate the interpretability of pLMs, offering a new perspective on their application in bioinformatics. Availability and implementation The source code is available for free at https://github.com/hmdlab/ARG-BERT. The output results of the model are published at https://waseda.box.com/v/ARG-BERT-suppl. [ABSTRACT FROM AUTHOR]

Photocatalysis is an effective technique to remove antibiotic residues from aquatic environments. Typical metal sulfides like Zn3In2S6 have been applied to a wide range of photocatalytic applications. However, there are currently no readily accessible methods to increase its antibiotic‐degrading activity. Here, a facile hydrothermal approach is developed for the preparation of flower‐like Zn3In2S6 with tunable sulfur lattice defects. Photogenerated carriers can be separated and transferred more easily when there is an adequate amount of lattice defects. Moreover, lattice defect‐induced electronic modulation enhances light utilization and adsorption properties. The modified Zn3In2S6 demonstrates outstanding photocatalytic degradation activity for levofloxacin, ofloxacin, and tetracycline. This work sheds light on exploring metal sulfides with sulfur lattice defects for enhancing photocatalytic activity. [ABSTRACT FROM AUTHOR]

Fish is an important source of animal food for humans because it contains essential nutrients that are easily absorbed by the body. However, the intensification of aquaculture can be harmful to the fish, making them more susceptible to reduced diseases and slow growth. The excessive use of antibiotics is a major concern as it can lead to the accumulation of antibiotic residue in the fish. As a result, many countries have banned the use of antibiotics in fish feed. Phytobiotics, derived from various plant sources, have gained importance as additives in fish feeds and are seen as a promising alternative to antibiotics due to the presence of various bioactive compounds that have the potential to enhance fish production and health. The bioactive compounds present in plant sources such as essential oils, microalgae, seaweed, medicinal plants, and seeds, like Aloe vera and licorice, offer a wide range of beneficial effects in promoting growth, antimicrobial activity, antioxidant properties, and immunostimulant. The main topics of this review highlighted the various sources of phytobiotics, their mode of action, their effects on growth performance, and their therapeutic effects in fish. While phytobiotics have low bioavailability, exposure to them has shown positive effects on organs and several tissues in fish. This present article is also designed to provide an insightful information about the administration of phytobiotics and its bioavailability in fish. Furthermore, this review offers an intuitive knowledge regarding various methods for extracting the phytobiotics. [ABSTRACT FROM AUTHOR]

Poultry farmers around the world utilize antibiotics, albeit indiscriminately, for both preventive and curative purposes. This study assessed tetracycline residues in chicken sampled from 4 poultry farms in Obio-Akpor Local Government Area in Rivers State, Nigeria. Liver, kidney, muscle and gizzard tissues were analyzed using High performance Liquid Chromatography (HPLC) for tetracycline, oxytetracycline and doxycycline residues. All samples reported residue levels within the WHO/FAO Maximum Residue Limits (MRLs) except for the liver sample in Farm 1 which was 895.04 µg/kg. Doxycycline recorded the least residue level within the range of 1.67 µg/kg to 9.30 µg/kg, while oxytetracycline and tetracycline recorded residue levels within the ranges of 1.671µg/kg to 98.662µg/kg and 0.886 to 895.037 µg/kg respectively. These results are indicative of the susceptibility of consumers to health hazards arising from antibiotic residues. It is therefore pertinent for Regulatory agencies to ensure compliance with effective and sustainable agricultural practices such as antibiotics withdrawal periods. [ABSTRACT FROM AUTHOR]

Affected by point and non-point source pollution, the Fenhe River Basin faces significant environmental challenges. This study aimed to analyze the distribution characteristics and influencing factors of antibiotics in the water and sediments of the Fenhe River Basin. Samples were collected from 23 sites within the basin, and 26 antibiotics from five different classes were detected and analyzed using high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS). The water–sediment partition coefficient (Kp) was calculated, and spatial analysis was conducted using geographic information system (GIS) technology. The results showed that 25 antibiotics were detected in the water, with concentrations ranging from 130 to 1615 ng/L, and 17 antibiotics were detected in the sediments, with concentrations ranging from 121 to 426 μg/kg. For quinolones (QNs), except for ofloxacin, all others could be calculated with overall high values of Kp ranging from 692 to 16,106 L/kg. The Kp values for QNs were generally higher in the midstream, with considerable point source pollution from industries and non-point source pollution from developed agriculture. The distribution of Kp is closely associated with risk. This study found that the Kp values of the antibiotics were influenced by various factors such as temperature, water flow, and the physicochemical properties of sediments. Correlation analysis revealed significant relationships between Kp and parameters such as river width, water depth, water quality (total nitrogen, total phosphorus, and chemical oxygen demand), and sediment pH and clay content. [ABSTRACT FROM AUTHOR]

Simple Summary: Considering the issue of antibiotic residues, developing equivalent alternatives in the context of banning antibiotics is key. Peptides are considered a satisfactory option, and the current study aims to demonstrate the effect of prophylactic fly maggot peptides on oxidative stress. Oral administration of fly maggot polypeptide can improve the oxidative stress induced by lipopolysaccharide combined with hemocoagulase, and improve the growth performance, antioxidant function, immune function, and intestinal barrier function of broilers. Recently, there has been fast-growing interest among researchers in discovering bioactive peptides from insects and evaluating their potential applications in livestock production. The present study aimed to assess the antioxidant properties and stability of fly maggot peptide (FMP) and its effects on Arbor Acres (AA) broilers' oxidative stress induced by lipopolysaccharide (LPS) and hemocoagulase (HC). A total of 108 one-day-old AA broilers were randomly divided into six groups: CG (normal saline, basal diet), DG (LPS + HC, basal diet), VG (DG + vitamin C 50 ug/kg), LPG (DG + FMP 5 mg/kg), MPG (DG + FMP 15 mg/kg), and HPG (DG + FMP 25 mg/kg). The results showed that the addition of FMP to the diet promoted LPS+ HC-induced increases in average daily gain (ADG), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC). Meanwhile, FMP regulated the intestinal morphology. Additionally, FMP decreased the increase in the contents of malondialdehyde (MDA), the relative weight of immune organs, interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). In conclusion, this research suggested that the addition of FMP can relieve the LPS+ HC-induced oxidative stress of AA broilers and the recommended dose of FMP is 25 mg/kg. This study presents a theoretical foundation for the addition of an FMP supplement for the purpose of protecting broilers' growth. [ABSTRACT FROM AUTHOR]

Dairy industry is leading in the structure of the food industry of Ukraine at the current stage. It consists of the butter production, cheese production, and milk-canning sub-sectors, as well as manufacturing of whole milk products, since the products of this industry occupy an important place in the consumption of the population. Therefore, research on regulation, toxicological and hygienic assessment of toxic contaminants of various origins must be conducted. The purpose of this study was to assess the cow's milk from Ukrainian farms for the presence of key safety indicators over the past 4 years, namely: residues of antibiotics, nitrofurans, coccidiostats, insecticides, fungicides, anthelmintics, corticosteroids, glucocorticosteroids, organochlorine and organophosphate pesticides, mycotoxins, toxic elements, radionuclides. In the period of 2020-2023, 7138 samples of cow's milk from native dairy enterprises from 25 regions of Ukraine were screened for the content of various dangerous substances and compounds from groups A and B which are declared in Directive 96/23/EC. It was established that the milk of cows from Ukraine meets current national standards and is not inferior in terms of quality and safety to EU indicators. Among the inconsistent results, it is worth highlighting the presence of Chloramphenicol in 0.85% of milk samples out of 234 tested for its content, which was 0.03% of the total number of samples, while the content of other investigated drugs and substances was below of the methods detection limit. [ABSTRACT FROM AUTHOR]

Antibiotic residues are widely recognized as major pollutants in the aquatic environment on a global scale. As a significant class of pharmaceutically active compounds (PhACs), antibiotics are extensively consumed worldwide. The primary sources of these residues include hospitals, municipal sewage, household disposal, and manures from animal husbandry. These residues are frequently detected in surface and drinking waters, sewage effluents, soils, sediments, and various plant species in countries such as China, Japan, South Korea, Europe, the USA, Canada, and India. Antibiotics are used medicinally in both humans and animals, with a substantial portion excreted into the environment as metabolites in feces and urine. With the advancement of sensitive and quantitative analytical techniques, antibiotics are consistently reported in environmental matrices at concentrations ranging from nanograms per liter (ng/L) to milligrams per liter (mg/L). Agricultural soils, in particular, serve as a significant reservoir for antibiotic residues due to their strong particle adsorption capacities. Plants grown in soils irrigated with PhAC-contaminated water can uptake and accumulate these pharmaceuticals in various tissues, such as roots, leaves, and fruits, raising serious concerns regarding their consumption by humans and animals. There is an increasing need for research to understand the potential human health risks associated with the accumulation of antibiotics in the food chain. The present reviews aims to shed light on the rising environmental pharmaceutical contamination concerns, their sources in the environment, and the potential health risks as well as remediation effort. To discuss the main knowledge gaps and the future research that should be prioritized to achieve the risk assessment. We examined and summarized the available data and information on the antibiotic resistance associated with antibiotic residues in the environment. As studies have indicated that vegetables can absorb, transport, and accumulate antibiotics in edible parts when irrigated with wastewater that is either inadequately treated or untreated. These residues and their metabolites can enter the food chain, with their persistence, bioaccumulation, and toxicity contributing to drug resistance and adverse health effects in living organisms. [ABSTRACT FROM AUTHOR]

Antimicrobial residues in milk pose a significant risk to human health, prompting regulatory bodies to establish safety limits to protect consumers. While traditional screening methods have primarily targeted single analytes or classes, recent advancements in multiclass screening techniques offer more comprehensive and efficient monitoring. This review highlights these advancements and focuses on methods developed since 2010, such as microbial inhibition tests, immunoassays, biosensors and LC–MS‐based approaches. It also discusses the strengths and limitations of these techniques, as well as the challenges associated with analysing milk's complex matrix, emphasising the ongoing need for innovation in food safety practices. [ABSTRACT FROM AUTHOR]

Beta‐lactam antibiotics (BLAs) are not only important in animal husbandry but are also unavoidable contaminants in milk. The gradual accumulation of BLAs in the human body due to increased milk intake can cause direct harm to human health. Therefore, developing selective and sensitive methods for rapid BLA detection is essential. In this study, a specific electrochemical receptor sensor based on penicillin‐binding proteins (PBPs) was established. PBP1a and PBP2x, which have high affinity and specificity for the six BLAs, were used as the receptors and their performance was compared. The sensor was fabricated by sequentially immobilizing a graphene/chitosan nanocomposite, penicillin G (PENG), and Nafion on the surface of a glassy carbon electrode using a convenient casting approach. The mechanism of the electrochemical receptor sensor involved direct competitive inhibition of the binding of the graphene/chitosan/PENG composite to the PBPs by the free BLAs in the solution. The PENG‐PBPs complex resisted the interaction between [Fe(CN)6]3−/4− ions and the glassy carbon electrode, thereby decreasing the current signal of the BLAs. The BLA concentration was quantified using differential pulse voltammetry mode. The graphene/PENG/chitosan/Nafion‐based electrochemical assay demonstrated good selectivity against other antibiotic residues frequently detected in milk. Under optimized conditions, the detection limits of this method ranged from 0.24 to 1.39 ng·mL−1, which are much lower than the maximum residue limits set by China and the EU. Moreover, the sensor showed a satisfactory recovery rate of 98.8 ± 5.8%–103.2 ± 7.1%. The total analysis time involved only one incubation step, and the entire analysis could be completed within 35 min. [ABSTRACT FROM AUTHOR]

The "antenna effect" is one of the most important energy transfer modes in lanthanide light-emitting polymers. In this study, novel luminescent nanostructured coordination polymers (Eu–PCP) were synthesized in one step using Eu3+ as the central metal ion and 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin (TCPP) as the organic ligand. The unique "antenna effect" observed between Eu3+ and TCPP leads to a substantial improvement in the electrochemiluminescence (ECL) emission efficiency. Eu–PCP exhibits good cathodic ECL characteristics. Additionally, Au@SnS2 nanosheets exhibit favorable electrical conductivity, biocompatibility, and a significant specific surface area. This makes them a suitable choice as substrate materials for the modification of electrode surfaces and capturing antigens. Being well known, the development of sensitive and rapid methods to detect chloramphenicol is essential for food safety. Based on this, we report a novel competitive electrochemiluminescence immunoassay to achieve ultra-sensitive and highly specific detection of chloramphenicol. The linear range was 0.0002–500 ng mL−1 and the detection limit was 0.09 pg mL−1. Apart from that, the experimental results proved that it provided a new analytical tool for the detection of antibiotic residues in food safety. [ABSTRACT FROM AUTHOR]

AbstractThe cultivation of tilapias, the third most farmed fish group globally, has been rapidly growing, especially in Southeast Asia. This surge in tilapia farming intensification has led to increased use of antibiotics to control bacterial diseases. This study investigated the safety implications of administering graded doses of enrofloxacin (ENF) at 0 (control), 10, 30, 50 and 100 mg/kg biomass/day orally to Oreochromis niloticus. The 43-day study comprised 7 days of pre-dosing, 15 days of ENF-dosing, and a 21-day recovery period with a periodical assessment of the biological responses of fish. The results revealed that the overdosed groups experienced up to 21% reduction in feed consumption, 11% mortalities, and adverse impacts on hematology, including a decrease in erythrocytes, and monocytes and an increase in leukocytes, thrombocytes, lymphocytes, and neutrophils. Haematological indices like mean corpuscular volume and mean corpuscular hemoglobin decreased, while mean corpuscular hemoglobin concentration increased. The plasma biochemical parameters including glucose and liver and kidney enzymes unveiled a significant dose- and time-dependent increase, while calcium and chloride levels decreased. Erythrocytes displayed several erythrocyte cellular and nuclear abnormalities. The frequency of micronucleus increased with dose and time, suggesting potential genotoxicity of ENF. Additionally, a dose-dependent increase in residues in the tissues with the highest accumulation in muscle was documented. Nevertheless, the recovery of the measured parameters upon dose termination indicated that the ENF-induced alterations are reversible. The study affirmed the safety of ENF at the recommended dose (10 mg) in O. niloticus and their adoptive responses to higher doses. [ABSTRACT FROM AUTHOR]

The global spread of multidrug-resistant Escherichia coli threatens human, animal, and environmental health. Despite the recognition of water bodies as potential reservoirs, research on antimicrobial resistance (AMR) and antibiotic residues in the Baltic Sea is limited. In this study, we examined the presence of ESBL-producing E. coli in surface water in northeastern Germany. We then conducted phenotypic and genotypic analyses of the strains, which encompassed AMR and heavy metal/metalloid tolerance, biofilm formation, and detailed plasmid examination. In addition, we used solid-phase extraction with ultra-high performance liquid chromatography and mass spectrometry to quantify antibiotic residues. Our analysis identified 30 ESBL-producing E. coli isolates, some of which were associated with clinically relevant high-risk clonal lineages. We detected antibiotic residues in the environmental samples, but the antibiotic concentrations were significantly lower compared to wastewater samples. These results suggest that the Baltic Sea surface water could serve as a reservoir for ESBL-producing E. coli and clinically relevant antibiotics. [ABSTRACT FROM AUTHOR]

Simple Summary: The dissemination of antimicrobial resistance (AMR) genes in economic animals affects food safety in our life cycle. The pig gut microbiome can be a reservoir of antimicrobial-resistant bacteria. As a result of the comparison of antimicrobial susceptibility profiles and the existence of ESBL genes, we found no difference between antibiotic- and nonantibiotic-using farms. We suggest a basal level of ESBL E. coli persistence on pig farms, which may not depend on antibiotic usage. Direct detection of antimicrobial-resistant genes from pig fecal samples reduced cumbersome bacterial culture processes. The enhanced sensitivity of the nPCR technique facilitates the surveillance of AMR genes, leading to effective control. The commensal Escherichia coli in the gut of pigs is a major reservoir of antimicrobial resistance and can result in possible transmission to humans through the food chain. Direct detection of E. coli from fecal samples is challenging and can be used as a bioindicator of antimicrobial resistance. This study aimed to compare the antimicrobial susceptibility profiles in commensal E. coli from antibiotic- and nonantibiotic-using pig farms and developed the direct detection of ESBL genes in pig fecal samples using nested PCR (nPCR) and multiplex PCR (mPCR) techniques. All direct genotypic results were validated with the results of PCR sequencing of isolated E. coli colonies. The ESBL-producing E. coli were found in 98.6% (145 isolates) and 96.6% (144 isolates) of antibiotic-using and nonantibiotic-using farms, respectively, predominantly CTX-M-55. The nPCR decreased the limit of detection (LOD) from sPCR about 100 times, and the lower LODs of 102, 101, and 1 CFU/mL were reached after incubating samples in an enrichment medium for 2, 4, and 8 h, respectively. The mPCR, sPCR, and nPCR techniques showed sensitivities of 30.15%, 69.85%, and 91.91%, respectively, compared to PCR sequencing. The stability and recycling of ESBL genes were independent of antibiotic usage in commensal E. coli originating in pig farms. [ABSTRACT FROM AUTHOR]

This article reports on the development of an atmospheric pressure filtering electrospray ionization (FESI) source – composed of a syringe, a filter, and the ESI source – for the determination of furazolidone, amoxicillin, nitrofurantoin, and nitrofural, common antibiotics. Spiked food samples were also analyzed; the analytes were placed in the filter head, and the flow rate of the extraction solution from the syringe pump was adjusted. Using a mixture of oleic acid and antibiotics, hydrophilic and hydrophobic filters were compared, demonstrating the advantage of FESI in reducing the background and noise for the mixture. The device simplifies not only the experimental procedure but also the sample pretreatment process. Additionally, the limits of detection of the antibiotics were determined to be 1 mg L−1 for furazolidone, amoxicillin, and nitrofurantoin and 2 mg L−1 for nitrofural. The linear regression coefficients (R2) of the antibiotic standard solutions were all ≥0.99. Furthermore, the spiked recovery rates of pork, chicken, and egg samples ranged from 73.3% to 107.5%, with relative standard deviations (RSDs) from 1.1% to 15.6%. This study uncovers the potential capabilities of ion trap mass spectrometry (ITMS) for the on-site detection of antibiotics and offers a new method for ensuring food safety. [ABSTRACT FROM AUTHOR]

Enramycin, a common growth promoter utilized in chickens and pigs, is sensitive against Gram-positive bacteria, and the maximum residue limit (MRL) of enramycin set up by is 30 µg/kg. However, the methods have been reported for detecting enramycin have failed to meet the accuracy requirements, with the required limit of quantification being higher than the MRL. To address this issue, we developed a high-sensitive and robust analytical method based on ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS), to determine enramycin residues in swine tissues, including liver, kidney, pork, and fat. The ENV cartridge was selected to cleanup and enrich analytes after being extracted using a mixture of 55% methanol containing 0.2 M hydrochloric acid. With comprehensively validation, this established method was found great linearity of enramycin in each tissue, with a coefficient of variation above 0.99. Satisfactory recoveries from four different spiking levels were acquired (70.99-101.40%) while the relative standard deviations were all below 9%. The limit of quantification of enramycin in the present study is 5 µg/kg in fat and 10 µg/kg in other tissues, meeting the requirements for conducting the corresponding safety evaluation study. This method was demonstrated with excellent specificity, stability, and high sensitivity. To conclude, this novel approach is sufficiently sensitive and robust for the safety evaluation of enramycin in food products. [ABSTRACT FROM AUTHOR]

The production of healthy animal-derived food entails the effective control of foodborne pathogens and strategies to mitigate microbial threats during rearing. Antibiotics have been traditionally employed in animal farming to manage bacterial infections. However, the prohibition of antibiotic growth promoters in livestock farming has brought significant changes in animal production practices. Although antibiotics are now restricted to treating and preventing bacterial infections, their overuse has caused serious public health issues, including antibiotic resistance and the presence of antibiotic residues in food and wastewater. Therefore, sustainable animal production is crucial in reducing the spread of antibiotic-resistant bacteria. Annually, 40–50% of fruit and vegetable production is discarded worldwide. These discards present significant potential for extracting value-added ingredients, which can reduce costs, decrease waste, and enhance the food economy. This review highlights the negative impacts of antibiotic use in livestock farming and stresses the importance of analyzing the challenges and safety concerns of extracting value-added ingredients from fruit and vegetable co-products at an industrial scale. It also explores the current trends in reducing antibiotic use in livestock, with a focus on Latin American contexts. Finally, the suitability of using value-added ingredients derived from fruit and vegetable co-products for animal feeds is also discussed. [ABSTRACT FROM AUTHOR]

A growing environmental concern revolves around the widespread use of medicines, particularly antibiotics, which adversely impact water quality and various life forms. The unregulated production and utilization of antibiotics not only affect non-targeted organisms but also exert significant evolutionary pressures, leading to the rapid development of antimicrobial resistance (AMR) in bacterial communities. To address this issue, global studies have been conducted to assess the prevalence and quantities of antibiotics in various environmental components including freshwater, ocean, local sewage, and fish. These studies aim to establish effective analytical methods for identifying and measuring antibiotic residues in environmental matrices that might enable authorities to establish norms for the containment and disposal of antibiotics. This article offers a comprehensive overview of methods used to extract antibiotics from environmental matrices exploring purification techniques such as liquid–liquid extraction, solid-phase extraction, green extraction techniques, and concentration methods like lyophilization and rotary evaporation. It further highlights qualitative and quantitative analysis methods, high-performance liquid chromatography, ultra-high-performance liquid chromatography, and liquid chromatography-tandem along with analytical methods such as UV–Vis and tandem mass spectrometry for detecting and measuring antibiotics. Urgency is underscored for proactive strategies to curb antibiotic contamination, safeguarding the integrity of aquatic ecosystems and public health on a global scale. [ABSTRACT FROM AUTHOR]

Large-scale poultry production in low- and middle-income countries may be a source of adulterated products (e.g., Salmonella contamination, antibiotic residues) that can be disseminated over wide areas. We employed a cross-sectional survey of 199 randomly selected poultry farms in Lagos State, Nigeria, to estimate the prevalence of non-typhoidal Salmonella (NTS), and biosecurity and antibiotic use practices. Pooled fecal samples were collected from laying chickens and from poultry handlers. Selective culture, biochemical assays, and PCR (invA) were used to isolate and confirm NTS isolates. NTS was detected at 14% of farms (28/199) and from 10% of farm workers (6/60). Multivariate logistic regression analysis indicated that antiseptic foot dips reduced the odds ratio (OR) for detecting NTS in chicken feces [OR: 0.55; 95% confidence interval (CI) 0.07–0.58]. Most farms (94.5%, 188/199) used antibiotics for treatment and prophylaxis, but no farms (0/199) exercised withdrawal before sale of products. Most farms (86.4%, 172/199) reported using antibiotic cocktails that included medically important colistin, ciprofloxacin, chloramphenicol, and gentamicin. Egg production in Lagos State relies heavily on antibiotics and antibiotic residues are likely passed to consumers through poultry products, but there is evidence that low-cost biosecurity controls are effective for limiting the presence of NTS on farms. [ABSTRACT FROM AUTHOR]

The emission of glyphosate and antibiotic residues from human activities threatens the diversity and functioning of the microbial community. This study examines the impact of a glyphosate-based herbicide (GBH) and common antibiotics on Gram-negative bacteria within the ESKAPEE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli). Ten strains, including type and multidrug-resistant strains for each species were analysed and eight antibiotics (cefotaxime, meropenem, aztreonam, ciprofloxacin, gentamicin, tigecycline, sulfamethoxazole-trimethoprim, and colistin) were combined with the GBH. While most combinations yielded additive or indifferent effects in 70 associations, antagonistic effects were observed with ciprofloxacin and gentamicin in five strains. GBH notably decreased the minimum inhibitory concentration of colistin in eight strains and displayed synergistic activity with meropenem against metallo-β-lactamase (MBL)-producing strains. Investigation into the effect of GBH properties on outer membrane permeability involved exposing strains to a combination of this GBH and vancomycin. Results indicated that GBH rendered strains sensitive to vancomycin, which is typically ineffective against Gram-negative bacteria. Furthermore, we examined the impact of GBH in combination with three carbapenem agents on 14 strains exhibiting varying carbapenem-resistance mechanisms to assess its effect on carbapenemase activity. The GBH efficiently inhibited MBL activity, demonstrating similar effects to EDTA (ethylenediaminetetraacetic acid). Chelating effect of GBH may have multifaceted impacts on bacterial cells, potentially by increasing outer membrane permeability and inactivating metalloenzyme activity. [ABSTRACT FROM AUTHOR]

Facing complex and variable emerging antibiotic pollutants, the traditional development of functional materials is a "trial-and-error" process based on physicochemical principles, where laborious steps and long timescales make it difficult to accelerate technical breakthroughs. Notably, natural biomolecular coronas derived from highly tolerant organisms under significant contamination scenarios can be used in conjunction with nanotechnology to tackling emerging contaminants of concern. Here, super worms (Tubifex tubifex) with high pollutant tolerance were integrated with nano-zero valent iron (nZVI) to effectively reduce the content of 17 antibiotics in wastewater within 7 d. Inspired by the synergistic remediation, nZVI-augmented worms were constructed as biological nanocomposites. Neither nZVI (0.3 to 3 g/L) nor worms (104 to 105 per liter) alone efficiently degraded florfenicol (FF, as a representative antibiotic), while their composite removed 87% of FF (3 µmol/L). Under antibiotic exposure, biomolecules secreted by worms formed a corona on and modified the nZVI particle surface, enabling the nano-bio interface greater functionality, including responsiveness, enrichment, and reduction. Mechanistically, FF exposure activated glucose-alanine cycle pathways that synthesize organic acids and amines as major metabolites, which were assembled into vesicles and secreted, thereby interacting with nZVI in a biologically response design strategy. Lactic acid and urea formed hydrogen bonds with FF, enriched analyte presence at the heterogeneous interface. Succinic and lactic acids corroded the nZVI passivation layer and promoted electron transfer through surface conjugation. This unique strategy highlights biomolecular coronas as a complex resource to augment nano-enabled technologies and will provide shortcuts for rational manipulation of nanomaterial surfaces with coordinated multifunctionalities. [ABSTRACT FROM AUTHOR]

Environmental pollution remains a major societal problem, leading to serious impacts on living organisms including humans. Human activities such as civilization, urbanization, and industrialization are major causes of pollution. Imposing stricter rules helps control environmental pollutant levels, creating a need for reliable pollutant monitoring in air, water, and soil. The application of traditional analytical techniques is limited in low-resource areas because they are sophisticated, expensive, and bulky. With the development of biosensors and microfluidics technology, environmental monitoring has significantly improved the analysis time, low cost, portability, and ease of use. This review discusses the fundamentals of portable devices, including microfluidics and biosensors, for environmental control. Recently, publications reviewing microfluidics and biosensor device applications have increased more than tenfold, showing the potential of emerging novel approaches for environmental monitoring. Strategies for enzyme-, immunoassay-, and molecular-based analyte sensing are discussed based on their mechanisms and applications. Microfluidic and biosensor platforms for detecting major pollutants, including metal ions, pathogens, pesticides, and antibiotic residues, are reviewed based on their working principles, advantages, and disadvantages. Challenges and future trends for the device design and fabrication process to improve performance are discussed. Miniaturization, low cost, selectivity, sensitivity, high automation, and savings in samples and reagents make the devices ideal alternatives for in-field detection, especially in low-resource areas. However, their operation with complicated environmental samples requires further research to improve the specificity and sensitivity. Although there is a wide range of devices available for environmental applications, their implementation in real-world situations is limited. This study provides insights into existing issues that can be used as references and a comparative analysis for future studies and applications. [ABSTRACT FROM AUTHOR]

Manure return to field has caused serious pollution of antibiotics and antibiotic resistance genes (ARGs) in soil-plant system, which has potential environmental risks. This paper summarized the distribution characteristics of antibiotics and ARGs in manure, soil, and plants and their influencing factors in soil-plant systems. The results showed that microorganisms prolonged exposure to low inhibitory concentrations of antibiotics and heavy metals was likely to induce the generation of ARGs, and there were significant geographical and species differences in antibiotic concentration and ARGs abundance in manure and improved soil. Generally, the residues of antibiotics and ARGs in manure and corresponding soil of pigs and chickens were higher than those of cattle. Moreover, antibiotics and antibioticresistant genes were more likely to accumulate in leafy and nightshade vegetables. Manure types, plant species, soil physicochemical properties, heavy metals, and microbial communities affected the distribution of antibiotics and ARGs in the soil-plant system. The migration of antibiotics from manure to soil and plants was greatly affected by the characteristics of antibiotics, and the concentration of antibiotics decreased continuously during transmedia migration. Bacterial communities significantly affect the migration of ARGs, diluted 100 to 1 000 times as they moved from soil to plant. For example, changes in soil microbial community structure will affect the species and abundance of ARGs in different cultivated crops. Research on the concentration control standards of antibiotics and ARGs in organic fertilizer and their migration mechanism needs to be intensified. This review aims to reveal the occurrence characteristics of ARGs in the soil-plant system and provide a basis for a scientific and effective response to the transmedia migration of microbial resistance. [ABSTRACT FROM AUTHOR]

In the evolving field of food safety, rapid and precise detection of antibiotic residues is crucial. This study aimed to tackle this challenge by integrating advanced inkjet printing technology with sophisticated microfluidic paper‐based analytical devices (µPADs). The µPAD design utilized "green" quantum dots synthesized via an eco‐friendly hydrothermal method using green white mulberry leaves as the carbon source, serving as the key fluorescent detection material. The action mechanism involved a photoinduced electron transfer system using red carbon dots (CDs) as electron donors and blue CDs combined with two‐dimensional layered molybdenum disulfide (MoS2) nanosheets as electron acceptors. This system could quickly detect antibiotics within 10 min in pork and water samples, demonstrating high sensitivity and recovery rates: 6.5 pmol/L at 99.75%–110% for sulfadimethoxine, 3.3 pmol/L at 99%–105% for sulfamethoxazole, and 8.5 pmol/L at 98.5%–105% for tetracycline. It achieved a relative standard deviation under 5%, ensuring reliability and reproducibility. The fabricated sensor offered a promising application for the rapid and efficient on‐site detection of antibiotic residues in food. [ABSTRACT FROM AUTHOR]

The increasing use of antibiotics in livestock poses environmental risks, leading to contamination of agricultural soils and propagation of microbial antibiotic-resistant genes (ARGs). This study examined the impacts of wood- and manure-derived biochar (BC) on antibiotic residues, ARGs, and microbial communities in sandy loam and clay loam soils amended with manure in Cynodon dactylon pastures. We hypothesized that BC amendments would influence the degradation of antibiotics and the structure of microbial communities based on their physicochemical properties and soil types. Our results demonstrated that wood BC reduced the concentrations of tetracycline and sulfonamides, particularly in sandy loam soil, due to its larger surface area and hydrophobic properties. In contrast, manure BC provided additional nutrients and supported atmospheric nitrogen-fixing microbial groups, especially in clay loam soil, while exhibiting variable efficiency in reducing antibiotic residues due to its lower surface area and higher ash content. These findings underscore the differential impacts of each BC type, emphasizing the need for tailored BC applications based on soil type to effectively mitigate antibiotic contamination and promote sustainable agricultural practices. In conclusion, wood BC was more effective in enhancing soil health by reducing antibiotic residues and improving microbial diversity, particularly in sandy loam soils, while manure BC was beneficial for nutrient cycling in clay loam soils. [ABSTRACT FROM AUTHOR]

Teicoplanin has been banned in the veterinary field due to the drug resistance of antibiotics. However, teicoplanin residue from the antibiotic abuse of humans and animals poses a threat to people's health. Therefore, it is necessary to develop an efficient way for the highly accurate and reliable detection of teicoplanin from humans, food, and water. In this study, novel imprinted quantum dots of teicoplanin were prepared based on boronate affinity-based precisely controlled surface imprinting. The imprinting factor (IF) for teicoplanin was evaluated and reached a high value of 6.51. The results showed excellent sensitivity and selectivity towards teicoplanin. The relative fluorescence intensity was inversely proportional to the concentration of teicoplanin, in the range of 1.0–17 μM. And its limit of detection (LOD) was obtained as 0.714 μM. The fluorescence quenching process was mainly controlled by a static quenching mechanism via the non-radiative electron-transfer process between QDs and the five-membered cyclic boronate esters. The recoveries for the spiked urine, milk, and water samples ranged from 95.33 to 104.17%, 91.83 to 97.33, and 94.22 to 106.67%, respectively. [ABSTRACT FROM AUTHOR]

Antimicrobial resistance (AMR) poses a significant threat to global health, powered by pathogens that become increasingly proficient at withstanding antibiotic treatments. This review introduces the factors contributing to antimicrobial resistance (AMR), highlighting the presence of antibiotics in different environmental and biological matrices as a significant contributor to the resistance. It emphasizes the urgent need for robust and effective detection methods to identify these substances and mitigate their impact on AMR. Traditional techniques, such as liquid chromatography-mass spectrometry (LC-MS) and immunoassays, are discussed alongside their limitations. The review underscores the emerging role of biosensors as promising alternatives for antibiotic detection, with a particular focus on electrochemical biosensors. Therefore, the manuscript extensively explores the principles and various types of electrochemical biosensors, elucidating their advantages, including high sensitivity, rapid response, and potential for point-of-care applications. Moreover, the manuscript investigates recent advances in materials used to fabricate electrochemical platforms for antibiotic detection, such as aptamers and molecularly imprinted polymers, highlighting their role in enhancing sensor performance and selectivity. This review culminates with an evaluation and summary of commercially available and spin-off sensors for antibiotic detection, emphasizing their versatility and portability. By explaining the landscape, role, and future outlook of electrochemical biosensors in antibiotic detection, this review provides insights into the ongoing efforts to combat the escalating threat of AMR effectively. [ABSTRACT FROM AUTHOR]

Background The AnticFast® Beta-lactams & Tetracyclines Combo Test Kit is a qualitative two-step (2 min + 5 min) rapid lateral flow assay to detect β-lactam (penicillins and cefalosporins) and tetracycline antibiotic residues in raw commingled cows' milk. Objective The method performance was evaluated according to Commission Implementing Regulation 2021/808 and Community Reference Laboratories Residues Guidelines for the Validation of Screening Methods for Residues of Veterinary Medicines and submitted for AOAC Performance Tested Methods SM certification. Methods The AnticFast Beta-lactams & Tetracyclines Combo Test Kit was evaluated for detection capability (CCβ), selectivity, false-positive results, repeatability, robustness, suitability for various milk types and milk compositions, milks from various species, and test kit consistency and stability. Samples included milks spiked at concentrations bracketing the EU maximum residue limits (MRLs) for β-lactams and tetracyclines as well as bulk farm and tanker milks. Results The AnticFast Beta-lactams & Tetracyclines Combo Test Kit is specific for the detection of residues of β-lactams and tetracyclines in milk and does not detect residues from other antibiotic families. Interference was seen with clavulanic acid, a β-lactamase inhibitor, which was expected. The test can detect (minimum a 95% detection) all residues of β-lactams (penicillins and cepfalosporins) and tetracyclines (parent drugs and their 4-epimers) present on the EU-MRL list for milk at their respective MRL except for desfuroylceftiofur and cefalexin, with a 95% detection only above the MRL. No false positives were detected in 599 blank samples (out of 305 farm and 301 tanker load samples) tested on both channels. Five real positives were detected and confirmed on the tetracycline channel for the farm milk samples, and two positives were detected and confirmed on the β-lactam channel for the tanker samples. Robustness testing indicated that the detection in high-protein raw cows' milk and heat-treated milk types (UHT, sterilized, and reconstituted milk powder) may be slightly hampered. For substances with a detection capability well below the MRL, this interference does not cause problems since detection at MRL remains guaranteed, but care should be taken for substances with a CCβ at or near their MRL. Diminished sample flow was seen for high-fat raw cows' milk and for all other cows' milk types other than raw milk and blank ewes' milk, so sample flow should always be verified for these milk types. Conclusions Results of this validation show that the AnticFast Beta-lactams & Tetracyclines Combo Test Kit is a reliable test for rapid screening of raw cows' milk for residues of β-lactam and tetracycline antibiotics. Highlights The AnticFast Beta-lactams & Tetracyclines Combo Test Kit is an easy, reliable, robust, and highly specific test for screening of β-lactam (penicillins and cefalosporins) and tetracycline antibiotic residues in milk with incubation at room temperature. In raw cows' milk, all tetracyclines are detected below 10 µg/kg. [ABSTRACT FROM AUTHOR]

The disposal and treatment of antibiotic residues is a recognized challenge due to the huge production, high moisture content, high processing costs, and residual antibiotics, which caused environmental pollution. Antibiotic residues contained valuable components and could be recycled. Using a one-step controllable pyrolysis technique in a tubular furnace, biochar (OSOBs) was produced without the preliminary carbonization step, which was innovative and time- and cost-saving compared to traditional methods. The main aim of this study was to explore the adsorption and removal efficiency of tetracycline (TC) in water using porous biochar prepared from oxytetracycline fermentation residues in one step. A series of characterizations were conducted on the prepared biochar materials, and the effects of biochar dosage, initial tetracycline concentration, reaction time, and reaction temperature on the adsorption capacity were studied. The experimental results showed that at 298 K, the maximum adsorption capacity of OSOB-3-700 calculated by the Langmuir model reached 1096.871 mg/g. The adsorption kinetics fitting results indicated that the adsorption of tetracycline on biochar was more consistent with the pseudo-second-order kinetic model, which was a chemical adsorption. The adsorption isotherm fitting results showed that the Langmuir model better described the adsorption process of tetracycline on biochar, indicating that tetracycline was adsorbed in a monolayer on specific homogeneous active sites through chemical adsorption, consistent with the kinetic conclusions. The adsorption process occurred on the surface of the biochar containing rich active sites, and the chemical actions such as electron exchange promoted the adsorption process. [ABSTRACT FROM AUTHOR]

Antibiotic resistant bacteria are prevalent environmental contaminants in freshwaters, and antibiotic resistance genes circulate throughout the urban water cycle. The increase of antibiotic resistant pathogens threatens public health through direct and indirect exposure, and natural resource managers need information on the spatial patterns of antibiotic resistant bacteria and environmental factors associated with their distribution to improve water quality monitoring and to better assess human, animal, and environmental health risks. We collected water and epilithic biofilm samples and measured physicochemical environmental variables at 29 sites distributed longitudinally in the Green-Duwamish River basin, Washington, USA. We characterized catchment-wide patterns of gram-negative fecal indicator bacteria and hypothesized that the presence of antibiotic resistance would be associated with environmental heterogeneity, bacterial primary ecology, stream compartment, and stream type. Antibiotic resistance was determined by microbial growth on selective media supplemented with 3 different antibiotics (ampicillin, chloramphenicol, or tetracycline). Phenotypic antibiotic resistance was positively associated with disturbance, but resistance to at least 1 antibiotic was also detected in undeveloped river segments, with an 83% overall detection rate (i.e., 24 out of 29 sites, 17 in the mainstem and 7 in tributaries). The most probable number of Escherichia coli was associated with higher levels of antibiotic resistance of non- E. coli coliforms across the basin (ρ = 0.38, p < 0.01) but was not associated with antibiotic resistance of E. coli. Phenotypic resistance was highest among non- E. coli coliforms in the water column of tributaries draining moderately to extensively developed subcatchments. Generalized linear mixed-effects model results showed that 18% of the variance in presence of antibiotic resistance was explained by the fixed effects (summed CV across environmental variables, stream type, primary ecology, and stream compartment), and when a spatial random effect was included, the model explained 27% of the variance. Our study provides new evidence that environmental factors and bacterial primary ecology are important underlying factors associated with spatial patterns of antibiotic resistant enteric coliforms. We used macroecological concepts and a riverscape approach to characterize the distribution of antibiotic resistance with methods applicable to municipalities. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. This study aimed to assess the effect of bulk tank milk (BTM), waste milk (WM), and pasteurized waste milk (PWM) on nutrient digestibility, ruminal and cecal fermentation, gastrointestinal tract (GIT) development, and antimicrobial resistance of fecal Escherichia coli from dairy calves at 2 periods (30 and 60 d of age). Calves were grouped according to BW, serum protein levels, and breed composition. Three treatments were included: BTM (n = 21), WM from cows under antibiotic treatment (n = 21), and PWM (WM submitted to HTST pasteurization; n = 21). A total of 63 calves were used, of which 18 animals (n = 6 per treatment) were evaluated in the period of 4 to 30 d, and 45 (n = 15 per treatment) from 4 to 60 d. During the experimental period, a daily intake of 6 L of milk was divided into 2 equal meals, with ad libitum access to water and starter. Milk and feed intakes were recorded daily. Apparent total-tract digestibility and nitrogen balance were conducted from 25 to 29 d of age (n = 6) and from 53 to 57 d of age (n = 15). Animals were slaughtered at 30 ± 1 and 60 ± 1 d of age for the assessment of ruminal and cecal fermentation and GIT development. Antimicrobial susceptibility testing was conducted at 1, 30, and 60 d of age (n = 15/treatment). Statistical analysis used a linear mixed-effects model for continuous outcomes and generalized linear models for single measurements (R software). Treatments WM and PWM had lower rumen pH, higher ruminal acetate concentration, larger reticulorumen and liver, and a higher prevalence of fecal-resistant E. coli compared with BTM at both 30 and 60 d. Up to 60 d, both BTM and WM treatments exhibited higher digestibility of ether extract and gross energy compared with the PWM, whereas WM and PWM treatments showed increased nitrogen intake and retention compared with the BTM treatment. These findings suggest that pasteurization of WM negatively affects nutrient digestibility and calf performance, while also affecting rumen development. Additionally, the use of milk containing antibiotic residue leads to the selection of resistant E. coli in the GIT over time. [ABSTRACT FROM AUTHOR]

To explore potential factors contributing to high fluoroquinolone resistance levels, it is essential to develop analytical methods capable of detecting residues and trace amounts of antibiotic use in broilers. The aim of the present study was to develop and in-house validate a sensitive UHPLC-MS/MS method capable of determining enrofloxacin (ENR) and flumequine (FLU) residues at slaughter age (day 45) when the animals were treated with these antimicrobials one day after hatching. Residue depletion of ENR and FLU in feathers was also assessed. Two experimental trials were performed, both consisting of 5 different treatment groups. In the first trial animals were treated with ENR and in the second one with FLU. The developed method was successfully validated and was found to be sensitive enough to detect residues of fluoroquinolones in the feathers up until slaughter age in all treatment groups. Average ENR concentration on day 45 was 10 ng g−1 feather after drinking water treatment, with all concentrations above the limit of quantification (LOQ) of 5 ng g−1 feather. For FLU average concentration on day 45 after drinking water administration was 4 ng g−1 feather, with an LOQ of 1 ng g−1 feather. Therefore, the method is suited for application to monitor fluoroquinolone use in broilers. [ABSTRACT FROM AUTHOR]

In regenerative endodontics, eradicating antibiotic residues from root canals is imperative, given their detrimental effects on human apical papilla stem cells. Previous antibiotic removal studies lacked precision in identifying types and quantities of residual antibiotics. High-performance liquid chromatography (HPLC) enhances sensitivity and specificity, enabling accurate detection and quantification of residual drugs. Using HPLC analysis, this study explored the influence of vehicles and irrigation solutions and methods on double antibiotic paste (DAP) removal from root canals. Two DAP formulations, each containing 5 mg/mL ciprofloxacin and metronidazole, were created using distinct vehicles: macrogol and propylene glycol (MP) or hydroxypropyl methylcellulose (HPMC). Subsequently, 5 μL of DAP was applied to 200 simulated immature teeth with open apices (n = 100 per formulation) and cultured for 28 days at 37°C. Samples were then divided into 11 groups (n = 20 per group), and canals were irrigated with 17% ethylenediaminetetraacetic acid or 10% citric acid, employing a positive pressure syringe or passive ultrasonic irrigation. The irrigation solution and dentin sample from each tooth were evaluated via HPLC for ciprofloxacin and metronidazole quantification. Citric acid exhibited significantly superior efficacy in antibiotic removal from root canals, with no observable effect of irrigation methods on drug removal. The HPMC-based DAP formulation significantly enhanced ciprofloxacin removal compared with MP-based DAP. For antibiotic paste removal from root canals, citric acid is effective, and HPMC is a preferable vehicle over MP. Overall, HPLC is a valuable method for detecting, removing, and quantifying residual antibiotics in root canals. [ABSTRACT FROM AUTHOR]

This review focuses on the crucial role of aquatic ecosystems in preserving biodiversity and the biosphere, as well as the connection between antimicrobial resistance (AMR) and these ecosystems. It provides an in-depth analysis of the link between ecological well-being and public health, helping readers understand this complex subject. Aquatic ecosystems are essential for maintaining biodiversity and ecological balance. Additionally, they act as primary reservoirs and pathways for the spread of antimicrobial resistance (AMR). These ecosystems risk antibiotic contamination through various sources, such as the release of antibiotics from animal and human waste, improper disposal of unused medications, and pharmaceutical industry waste management practices. The presence of antibiotic residues in these environments significantly speeds up the development of bacterial resistance. The global prevalence of antimicrobial resistance (AMR) is evident in freshwater bodies, tributaries, sewage waters, and wastewater treatment facilities. Antimicrobial resistance (AMR) is now a significant public health threat, compromising the effectiveness of many previously successful treatments against various pathogens. One notable and alarming aspect of antimicrobial resistance (AMR) is its rapid development, often occurring within 5–10 years after introducing antimicrobial drugs to the market. This acceleration is closely tied to bacteria's ability to thrive and adapt in the presence of antimicrobial agents and their residues in the environment. The implications of antimicrobial resistance (AMR) include treatment failures with long-term effects and a continuous increase in healthcare costs. This review comprehensively examines the intricate relationship between aquatic habitats, antibiotics, and the global challenge of antimicrobial resistance (AMR). It emphasizes the critical role of these ecosystems in preserving ecological diversity. It raises awareness about AMR's urgent public health issue, laying a foundation for understanding its extensive consequences. [ABSTRACT FROM AUTHOR]

The emergence of antimicrobial resistance due to antibiotics in the environment presents significant public health, economic, and societal risks. This study addresses the need for effective strategies to reduce antibiotic residues, focusing on ciprofloxacin degradation. Magnetic iron oxide nanoparticles (IO NPs), approximately 13 nm in size, were synthesized and functionalized with branched polyethyleneimine (bPEI) to obtain a positive charge. These IO-bPEI NPs were combined with negatively charged titanium dioxide NPs (TiO2@CA) to form magnetically photocatalytic IO-TiO2 nanocomposites. Characterization techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), infrared spectroscopy (IR), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), electrokinetic measurements, and a vibrating sample magnetometer (VSM), confirmed the successful formation and properties of the nanocomposites. The nanocomposites exhibited a high specific surface area, reduced mobility of photogenerated charge carriers, and enhanced photocatalytic properties. Testing the photocatalytic potential of IO-TiO2 with ciprofloxacin in water under UV-B light achieved up to 70% degradation in 150 min, with a degradation rate of 0.0063 min−1. The nanocomposite was magnetically removed after photocatalysis and successfully regenerated for reuse. These findings highlight the potential of IO-TiO2 nanocomposites for reducing ciprofloxacin levels in wastewater, helping curb antibiotic resistance. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Irrigation & Drainage is the property of Journal of Irrigation & Drainage Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: This study investigate the influence of season on the occurrence of adulteration by chemical preservatives and antibiotic residues (beta-lactam, tetracycline and sulfonamides) in the raw milk in the main towns of Khartoum State, Sudan. Methods: The milk samples (n= 270) were randomly collected from farms (90 samples), groceries (90 samples) and vendors (90 samples) during late summer and winter seasons, 135 samples during each season. The samples were placed in cool cracked ice and transported immediately to the laboratory for analysis. Results: Data revealed a high presence of the added water in the milk collected from vendors during late summer season (7.70± 9.46%). Also, hydrogen peroxide, starch and formalin were detected only in the milk obtained from vendors. However, the higher percentages of salt (32%) and ammonium sulphate (28%) were obtained in the milk samples collected from the groceries during late summer season, while the occurrenc of ammonium sulphate was 26% in the samples collected from vendors during winter season. On the other hand, 93% of the milk samples examined during late summer season were positive for the occurrence of antibiotic residues compared to 55% that was detected during winter season. The highest percentage of antibiotic residues (26%) was found in milk from groceries during the winter season. Milk samples from vendors during late summer season contained beta-lactam, tetracycline, and sulfonamide in 19%, 13%, and 1% of samples, respectively, while these figures were 20%, 2%, and 3%, respectively, during winter season. Conclusions: The addition of water followed by the presence of salt and antibiotics are the most adulterants found in raw milk sold in Khartoum State especially from vendors during late summer season. [ABSTRACT FROM AUTHOR]

Yancheng coastal wetland, the largest coastal wetland in the west coast of the Pacific Ocean and the margin of the Asian continent, has significant environmental, economic and social effects on local human beings. The extensive contamination and potential risk of quinolone antibiotics (QNs) on local aquaculture and human health are still not clear until now. In this study, 52 surface sediment samples were collected to investigate the contamination status and polluted sources, and evaluate ecological risks of QNs in the south of Yancheng coastal wetland. The total contents of QNs ranged from 0.33 to 21.60 ng/g dw (mean value of 4.51 ng/g dw), following the detection frequencies of QNs ranging from 19.23 to 94.23%. The highest content of QNs occurred around an aquaculture pond dominated by flumequine. The total organic carbon contents of sediment were positively correlated with sarafloxacin and lomefloxacin (p < 0.05), indicating the enhanced absorption of these QNs onto sediments. Partial QNs, such as lomefloxacin, enrofloxacin, sarafloxacin and flumequine, presented the homology features originating from the emission of medical treatment and aquaculture. There was no potential risk of QNs to human beings but a potential risk to aquatic organisms (algae > plant > invertebrate). Totally, the management and protection of Yancheng coastal wetland should be of concern with aquaculture as the important industry. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study evaluated the efficiency of aerobic granular sludge (AGS) technology in treating wastewater contaminated with tetracycline (TC), a common antibiotic. AGS was cultivated under a TC pressure gradient ranging from 5 mg/L to 15 mg/L and compared with conventional wastewater conditions. The results demonstrated that AGS achieved high removal efficiencies and exhibited robust sedimentation performance, with significant differences in average particle sizes observed under both conditions (618.6 μm in TC conditions vs. 456.4 μm in conventional conditions). Importantly, exposure to TC was found to alter the composition and production of extracellular polymeric substances (EPSs), thereby enhancing the structural integrity and functional stability of the AGS. Additionally, the selective pressure of TC induced shifts in the microbial community composition; Rhodanobacter played a crucial role in EPS production and biological aggregation, enhancing the structural integrity and metabolic stability of AGS, while Candida tropicalis demonstrated remarkable resilience and efficiency in nutrient removal under stressful environmental conditions. These findings underscore the potential of AGS technology as a promising solution for advancing wastewater treatment methods, thus contributing to environmental protection and sustainability amid growing concerns over antibiotic contamination. [ABSTRACT FROM AUTHOR]

The article explores the science and practice of electrospinning, tracing its history and examining the factors that influence fiber morphology, including the solution composition, processing conditions, and environmental conditions. It offers a thorough examination of electrospinning fundamentals geared toward generating an appropriate platform for creating tailored nanofibers for sensing applications in the food industry. These nanofibers play a critical role in food analysis, sensing, and traceability, and we highlight their effectiveness in identifying pesticide residues, food components, antibiotics, heavy metals, and foodborne pathogens as well as in monitoring the freshness and spoilage of food. The use of electrospinning technology is poised to make sensing platforms more accessible, affordable, and widespread, thereby significantly contributing to the improvement of food quality and safety monitoring practices. [ABSTRACT FROM AUTHOR]