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7 results on '"API Staph"'

1. Ocular Surface Infection and Antimicrobials.

Author

Willcox, Mark, Dutta, Debarun, Stapleton, Fiona, and Willcox, Mark

Subjects
Bacteriology (non-medical), API Staph, Bdellovibrio, Biolog, Candida, DNA sequencing, Enterobacterales, Fusarium, MPDS susceptibility, MSSA, Mel4 peptide, Micavibrio, Panton-Valentine leukocidin, Pseudomonas, Pseudomonas aeruginosa, Serratia marcescens, Staphyloccus aureus, Staphylococcus, Staphylococcus aureus, antibiotic, antibiotic resistance, antibiotic susceptibility, antimicrobial contact lens, antimicrobial peptides, bacteria, bacterial infection, biocompatibility, biofilms, blepharitis, ciprofloxacin, clinical trail, coagulase-negative staphylococci, combined effect, comfort, conjunctivitis, contact lens, contact lenses, cornea, corneal infiltrative events, endophthalmitis, epithelium, extended wear, eye infections, infection, keratitis, metabolomics, microbial keratitis, misuse of antibiotics, multilocus sequence typing, n/a, ocular infection, ocular infectious isolates, ocular surface, orthokeratology, predatory bacteria, pulsed-field gel electrophoresis, questionnaire, sodA gene, stress response system, transcription factor, ultraviolet C, virulence factors, whole genome sequencing
Abstract

Summary: This reprint collates papers from a Special Issue of the journal Antibiotics, which was entitled "Ocular surface Infection and Antimicrobials". The papers cover aspects such as common microbes which cause ocular infections and their susceptibility to antibiotics; how guidelines for antibiotic use can translate to improved patient compliance; how bacteria respond to antibiotics; and lastly, new treatments and ways of preventing ocular surface infections.

2. Comparison of methods for the identification of coagulase-negative staphylococci

Author

Maria de Lourdes RS Cunha, Yuri K Sinzato, and Liciana VA Silveira

Subjects
coagulase-negative staphylococci, methods, identification, API Staph, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216
Abstract

Coagulase-negative staphylococci (CNS) species identification is still difficult for most clinical laboratories. The scheme proposed by Kloos and Schleifer and modified by Bannerman is the reference method used for the identification of staphylococcal species and subspecies; however, this method is relatively laborious for routine use since it requires the utilization of a large number of biochemical tests. The objective of the present study was to compare four methods, i.e., the reference method, the API Staph system (bioMérieux) and two methods modified from the reference method in our laboratory (simplified method and disk method), in the identification of 100 CNS strains. Compared to the reference method, the simplified method and disk method correctly identified 100 and 99% of the CNS species, respectively, while this rate was 84% for the API Staph system. Inaccurate identification by the API Staph method was observed for Staphylococcus epidermidis (2.2%), S. hominis (25%), S. haemolyticus (37.5%), and S. warneri (47.1%). The simplified method using the simple identification scheme proposed in the present study was found to be efficient for all strains tested, with 100% sensitivity and specificity and proved to be available alternative for the identification of staphylococci, offering, higher reliability and lower cost than the currently available commercial systems. This method would be very useful in clinical microbiology laboratory, especially in places with limited resources.

Published
2004
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3. Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections

Author

Shannon V. Nayyar, John E Romanowski, Vishal Jhanji, Regis P. Kowalski, Robert M. Q. Shanks, and Eric G. Romanowski

Subjects
0301 basic medicine, Microbiology (medical), coagulase-negative staphylococci, genetic structures, medicine.drug_class, sodA gene, 030106 microbiology, Antibiotics, RM1-950, Biochemistry, Microbiology, Article, Keratitis, 03 medical and health sciences, API Staph, 0302 clinical medicine, Endophthalmitis, Biolog, medicine, conjunctivitis, Pharmacology (medical), DNA sequencing, General Pharmacology, Toxicology and Pharmaceutics, Blepharitis, eye infections, business.industry, cons, Eye infection, medicine.disease, Infectious Diseases, keratitis, endophthalmitis, 030221 ophthalmology & optometry, blepharitis, Vancomycin, sense organs, Therapeutics. Pharmacology, antibiotic susceptibility, business, Empiric therapy, medicine.drug
Abstract

Coagulase-negative staphylococci (CoNS) are frequently occurring ocular opportunistic pathogens that are not easily identifiable to the species level. The goal of this study was to speciate CoNS and document antibiotic susceptibilities from cases of endophthalmitis (n = 50), keratitis (n = 50), and conjunctivitis/blepharitis (n = 50) for empiric therapy. All 150 isolates of CoNS were speciated using (1) API Staph (biochemical system), (2) Biolog GEN III Microplates (phenotypic substrate system), and (3) DNA sequencing of the sodA gene. Disk diffusion antibiotic susceptibilities for topical and intravitreal treatment were determined based on serum standards. CoNS identification to the species level by all three methods indicated that S. epidermidis was the predominant species of CoNS isolated from cases of endophthalmitis (84–90%), keratitis (80–86%), and conjunctivitis/blepharitis (62–68%). Identifications indicated different distributions of CoNS species among endophthalmitis (6), keratitis (10), and conjunctivitis/blepharitis (13). Antibiotic susceptibility profiles support empiric treatment of endophthalmitis with vancomycin, and keratitis treatment with cefazolin or vancomycin. There was no clear antibiotic choice for conjunctivitis/blepharitis. S. epidermidis was the most frequently found CoNS ocular pathogen, and infection by other CoNS appears to be less specific and random. Antibiotic resistance does not appear to be a serious problem associated with CoNS.

Published
2021

4. IDENTIFICATION OF METHICILLIN-RESISTANT, COAGULASE-NEGATIVE STAPHYLOCOCCUS STRAINS USING THE API STAPH COMMERCIAL SYSTEM.

Author

Colcieru, Mircea, Jakab, Endre, Hilma, Geta, and Popescu, Octavian

Subjects
METHICILLIN resistance, COAGULASE, STAPHYLOCOCCAL diseases, POLYMERASE chain reaction
Abstract

Community-acquired and hospital-acquired systemic staphylococcal infections are two major causes of mortality worldwide. A total of 125 staphylococcal isolates collected in Sibiu between July 2008 and January 2010 were used in this study. Twenty three of them were confirmed using the mecA/nucA duplex PCR technique; ten strains contains the nucA gene and were considered Staphylococcus aureus. The rest of 13 methicillin-resistant strains were tested using the API Staph commercial identification system (bioMérieux, France). [ABSTRACT FROM AUTHOR]

Published
2010

5. Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections.

Author

Romanowski, John E., Nayyar, Shannon V., Romanowski, Eric G., Jhanji, Vishal, Shanks, Robert M. Q., and Kowalski, Regis P.

Subjects
ANTIBIOTICS, DNA sequencing, COAGULASE, STAPHYLOCOCCUS, GENETIC speciation, INFECTION
Abstract

Coagulase-negative staphylococci (CoNS) are frequently occurring ocular opportunistic pathogens that are not easily identifiable to the species level. The goal of this study was to speciate CoNS and document antibiotic susceptibilities from cases of endophthalmitis (n = 50), keratitis (n = 50), and conjunctivitis/blepharitis (n = 50) for empiric therapy. All 150 isolates of CoNS were speciated using (1) API Staph (biochemical system), (2) Biolog GEN III Microplates (phenotypic substrate system), and (3) DNA sequencing of the sodA gene. Disk diffusion antibiotic susceptibilities for topical and intravitreal treatment were determined based on serum standards. CoNS identification to the species level by all three methods indicated that S. epidermidis was the predominant species of CoNS isolated from cases of endophthalmitis (84–90%), keratitis (80–86%), and conjunctivitis/blepharitis (62–68%). Identifications indicated different distributions of CoNS species among endophthalmitis (6), keratitis (10), and conjunctivitis/blepharitis (13). Antibiotic susceptibility profiles support empiric treatment of endophthalmitis with vancomycin, and keratitis treatment with cefazolin or vancomycin. There was no clear antibiotic choice for conjunctivitis/blepharitis. S. epidermidis was the most frequently found CoNS ocular pathogen, and infection by other CoNS appears to be less specific and random. Antibiotic resistance does not appear to be a serious problem associated with CoNS. [ABSTRACT FROM AUTHOR]

Published
2021
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6. Entwicklung und Evaluierung einer qualitätskontrollierten 16S-rDNADatenbank zur Identifizierung von Vertretern der Unterordnung Micrococcineae (triv. Mikrokokken)

Author

Lee, S. (Sea-Hyun), Becker, K. (Karsten), and Universitäts- und Landesbibliothek Münster

Subjects
Medicine and health, 16S-rDNA-Sequenzierung, API STAPH, VITEK 2, RIDOM, Micrococcineae, Micrococcus, ddc:610
Abstract

Anhand einer klinischen „Mikrokokken"-Stammsammlung wurde die molekulare Identifizierung basierend auf variablen Abschnitten des 16S-rRNA-Gens mit zwei auf phänotypischen Nachweismethoden beruhenden Identifizierungssystemen (API STAPH, VITEK 2) verglichen. Die 16-rDNA-Sequenzanalyse identifizierte 38 der 74 klinischen Isolate (51,4%) innerhalb einer Sequenzübereinstimmung von mindestens 99%. Die 16S-rDNA-Sequenzanalyse und API-STAPH kamen zu 31 (41,9%), Sequenzanalyse und VITEK 2 zu 28 übereinstimmmenden Identifizierungen (37,8%). Insgesamt gab es für 22 der 74 klinischen Isolate (29,7%) ein übereinstimmendes Ergebnis aller drei Nachweismethoden. Eine qualitätskontrollierte 16S-rDNA-Datenbank zur Identifizierung von Vertretern der Unterordnung Micrococcineae konnte erfolgreich etabliert werden. Im Gegensatz zu den in der Arbeit eingesetzten phänotypischen Nachweismethoden hat sich die 16S-rDNA-Sequenzanalyse als schnellere und exakte Methode zur Identifizierung von „Mikrokokken" bewährt und konnte Hinweise auf bisher nicht beschriebene Vertreter der Micrococcineae unter den klinischen Isolaten aufzeigen.

Published
2007

7. Comparison of methods for the identification of coagulase-negative staphylococci

Author

Liciana Vaz de Arruda Silveira, Maria de Lourdes Ribeiro de Souza da Cunha, Yuri K. Sinzato, Departamento de Microbiologia e Imunologia, and Universidade Estadual Paulista (Unesp)

Subjects
Microbiology (medical), Coagulase, coagulase-negative staphylococci, Identification scheme, lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, Staphylococcus, lcsh:QR1-502, Computational biology, Biology, Sensitivity and Specificity, lcsh:Microbiology, Microbiology, methods, API Staph, Staphylococcus epidermidis, Species identification, Humans, biology.organism_classification, bacterial infections and mycoses, Bacterial Typing Techniques, Culture Media, Clinical microbiology, identification, Lower cost, Identification (biology), Limited resources
Abstract

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:45:32Z No. of bitstreams: 1 S0074-02762004000800012.pdf: 56650 bytes, checksum: dd15abe0c90e854a9025753fa1681c37 (MD5) Made available in DSpace on 2013-08-22T18:45:32Z (GMT). No. of bitstreams: 1 S0074-02762004000800012.pdf: 56650 bytes, checksum: dd15abe0c90e854a9025753fa1681c37 (MD5) Previous issue date: 2004-12-01 Made available in DSpace on 2013-09-30T19:34:37Z (GMT). No. of bitstreams: 2 S0074-02762004000800012.pdf: 56650 bytes, checksum: dd15abe0c90e854a9025753fa1681c37 (MD5) S0074-02762004000800012.pdf.txt: 28415 bytes, checksum: e69f3f6064bbea9978571225c804c0a3 (MD5) Previous issue date: 2004-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:09:25Z No. of bitstreams: 2 S0074-02762004000800012.pdf: 56650 bytes, checksum: dd15abe0c90e854a9025753fa1681c37 (MD5) S0074-02762004000800012.pdf.txt: 28415 bytes, checksum: e69f3f6064bbea9978571225c804c0a3 (MD5) Made available in DSpace on 2014-05-20T15:09:25Z (GMT). No. of bitstreams: 2 S0074-02762004000800012.pdf: 56650 bytes, checksum: dd15abe0c90e854a9025753fa1681c37 (MD5) S0074-02762004000800012.pdf.txt: 28415 bytes, checksum: e69f3f6064bbea9978571225c804c0a3 (MD5) Previous issue date: 2004-12-01 Coagulase-negative staphylococci (CNS) species identification is still difficult for most clinical laboratories. The scheme proposed by Kloos and Schleifer and modified by Bannerman is the reference method used for the identification of staphylococcal species and subspecies; however, this method is relatively laborious for routine use since it requires the utilization of a large number of biochemical tests. The objective of the present study was to compare four methods, i.e., the reference method, the API Staph system (bioMérieux) and two methods modified from the reference method in our laboratory (simplified method and disk method), in the identification of 100 CNS strains. Compared to the reference method, the simplified method and disk method correctly identified 100 and 99% of the CNS species, respectively, while this rate was 84% for the API Staph system. Inaccurate identification by the API Staph method was observed for Staphylococcus epidermidis (2.2%), S. hominis (25%), S. haemolyticus (37.5%), and S. warneri (47.1%). The simplified method using the simple identification scheme proposed in the present study was found to be efficient for all strains tested, with 100% sensitivity and specificity and proved to be available alternative for the identification of staphylococci, offering, higher reliability and lower cost than the currently available commercial systems. This method would be very useful in clinical microbiology laboratory, especially in places with limited resources. Departamento de Microbiologia e Imunologia Universidade Estadual Paulista Instituto de Biociências Departamento de Bioestatística Universidade Estadual Paulista Instituto de Biociências Departamento de Bioestatística

Published
2004

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