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This study was performed in Egypt's Sakha Agriculture Research Station during the seasons 2015-2018 to study the efficiency of biparental progenies derived from inter-population random mating in F2 generation in a cotton cross. Intercrossing is allowed in cotton breeding populations. Although the best lines may include harmful alleles, thus early segregating generation recombination and hybridization are required. The F2 generation of the cotton cross (Giza70 TNB1), which obtained from Agriculture Research Center, Egypt, consisted of five male and eight female plants, was used to test biparental progeny from random interpopulation mating. About 28 and 24 families in BIPS1 and BIPS2 had their original parents were examined. All genotypes were assessed for agricultural and fiber qualities. The mean of families (BIPs2) was higher than BIPs1 for all measured metrics except lint yield, percentage, and uniformity ratio. BIPS2 families had more genotypic coefficients of variation (GCV) for features than BIPS1. Intercrossing affected correlation coefficients of BIPS1 and BIPS2 genotypes. Seed cotton yield and lint index genotypic correlations became considerable. Seed cotton yield estimated by seed index and first fruiting node location were altered from negative to positive. Among them, No.5, No.7, No.8, No.18, and No.19 had large yields and early ripening, as shown by their components. The top five families chosen for fiber length had gain values of 3.373% to 8.961% and 5.919 to 7.781% for fiber strength. The No.18 family exhibited good seed cotton production and fiber length. Intermating and self-pollination (BIPS2) may utilize non-additive gene effects and accumulate trait-influencing alleles. Continuing to select and evaluate families based on their large-scale reproductive performance will enhance the likelihood of producing genotypes with desired features. In conclusion, this study underscores the utility of continued selection based on discerned genetic parameters, genotypic correlations, and observed gains in facilitating the ongoing improvement of cotton breeding programs. By providing valuable insights into the intricacies of genetic variability and trait associations, this research holds implications not only for the advancement of cotton breeding practices but also for the broader agricultural landscape on both national and global scales. [ABSTRACT FROM AUTHOR]

2,4-Dichlorophenoxyacetic acid (2,4-D) is the third most commonly used herbicide all over the world. There is a contradicted opinion about its toxicity and its half life in the environment. In this study the most effective method of its degradation and bioremediation has been studied. Two microbial consortia capable of utilizing 2,4-D as a sole source of carbon were isolated from the Egyptian environment. One of the microbial consortia interestingly contained a certain kind of protozoa as one of the mixed consortia members. Degradation of 2,4-D by the microbial consortia was affected by 2,4-D initial concentration, agitation, pH of the medium and temperature. The two consortia were able to degrade up to 700 mg l−1 of 2,4-D. Pre-treatment with UV radiations in the presence of photocatalyst such as TiO2 accelerates the biodegradation process. The toxic non biodegradable concentration of 2,4-D which was found to be the 800 mg l−1, was degraded by pre-treatment with UV/TiO2 and a subsequent microbial inoculation. The combined treatment proved to be an efficient mean of biodegradation and detoxification of toxic non biodegradable concentrations of 2,4-D.

Introduction: Heat stress caused by global warming adversely affects wheat yield through declining most nutritional quality attributes in grains, excluding grain protein content., Methods: This research investigated the biochemical, physiological, and antioxidant responses of wheat plants under heat stress, focusing on the role of plant growth-promoting bacteria ( Micromonospora sp.). Two wheat genotypes were studied: one heat-sensitive and one heat-tolerant, examining their responses to heat stress with and without bacterial inoculation., Results: Under heat stress, the sensitive cultivar experienced significant reductions in photosynthesis rate, chlorophyll content, and RuBisCO activity (57-61%), while the tolerant cultivar had milder reductions (24-28%). Micromonospora sp. treatment notably improved these parameters in the sensitive cultivar (+48-78%), resulting in a substantial increase in biomass production (+43-53%), which was not seen in the tolerant cultivar. Additionally, oxidative stress markers (H 2 O 2 and MDA) were elevated more in the sensitive cultivar (82% and 90% higher) compared to the tolerant one. Micromonospora sp. treatment effectively reduced these markers in the sensitive cultivar (-28% and -27%). Enhanced activity of antioxidant enzymes and ASC-GSH pathway enzymes was particularly evident in Micromonospora sp.-treated sensitive plants. Carbohydrate metabolism shifted, with increased soluble sugars and significant rises in sucrose content in Micromonospora sp.-treated plants under stress., Discussion: The higher soluble sugar levels facilitated amino acid synthesis, contributing to biosynthesis of secondary metabolites, including flavonoids, polyphenols, and anthocyanins. This was reflected in increased activity of phenylalanine ammonia-lyase, cinnamate (CA) 4-hydroxylase, and chalcone synthase enzymes, indicating the activation of phenylpropanoid pathways. Overall, the findings suggest that Micromonospora sp. can mitigate heat stress effects by enhancing photosynthetic efficiency, antioxidant defense, and metabolic adaptations in heat-sensitive wheat cultivars., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Hassan, Ahmed, Sheteiwy, Alhaj Hamoud, Okla, AlGarawi, Maridueña-Zavala, Alaraidh, Reyad and Abdelgawad.)

Vitis jacquemontii R. Parker is a wild grape traditionally used by indigenous people as a substitute for cultivated grapes. However, its therapeutic effects have not been extensively studied. In this study, we investigated the antioxidant, anticholinesterase, analgesic, and antidepressant properties of V . jacquemontii . The antioxidant potential of this wild fruit plant was evaluated using two widely recognized assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-asino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). In-vitro anticholinesterase effects were determined by assessing butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) inhibition. The analgesic activity was assessed through writhing and tail immersion test models, while the antidepressant effect was evaluated using forced swimming and tail suspension test models. Results revealed the exceptional potential of V . jacquemontii as a valuable natural resource. The fruit extract (VJF-Crd) demonstrated remarkable free radical scavenging abilities, with an impressive IC 50 value of 34.96 μg/mL for DPPH and 56.48 μg/mL for ABTS. The leaf extract (VJL-Crd) also exhibited considerable antioxidant properties, with IC 50 values of 73.68 μg/mL for DPPH and 86.72 μg/mL for ABTS. Furthermore, VJF-Crd and VJL-Crd extracts displayed potent inhibitory activity against cholinesterase enzymes, with VJF-Crd demonstrating strong inhibition and VJL-Crd showing moderate inhibition. In terms of analgesia, these extracts exhibited dose-dependent responses in various pain models, with significant protection against acetic acid-induced writhing and tail immersion, showcasing their potential as natural pain relievers. Moreover, both VJF-Crd and VJL-Crd extracts displayed a notable decrease in immobility in the forced swimming and tail suspension test models, indicating their potential as natural antidepressants. These findings underscore the untapped potential of V . jacquemontii as a source of valuable chemical constituents. The isolation and identification of phyto-constituents from this plant hold promise for new bioactive compounds, particularly in pain management. This study sheds light on the multifaceted medicinal attributes of V . jacquemontii and opens new avenues for developing natural remedies for different ailments, especially pain management., Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nasrullah Khan reports financial support, article publishing charges, and writing assistance were provided by 10.13039/501100002383King Saud University, Riyadh, Saudi Arabia. Nasrullah Khan reports a relationship with University of Karachi that includes: board membership. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors. Published by Elsevier Ltd.)

Background: It is imperative to enhance the quality of sprouts since they are a rich source of various primary and secondary metabolites. The objective of this work was to examine how multiwalled carbon nanotubes (MWCNTs) priming at various concentrations affected the nutritional qualities of four horticultural plants (T. foenum-graecum, L. grandiflorum, L. sativum and A. graveolens) and their sprouting processes., Results: Among the four applied concentrations (10-60 mgL -1 ), MWCNTs at 10 and 40 mg L⁻¹ induced the highest biomass accumulation in L. grandiflorum and T. foenum-graecum, respectively, while 60 mg L⁻¹ was most effective for L. sativum and A. graveolent. MWCNTs induced growth by enhancing photosynthesis, as shown by increased chlorophyll content and rubisco activity, which rose by 27%, 17%, 23% and 12% in T. foenum-graecum, L. grandiflorum, L. sativum, and A. graveolens, respectively. Enhanced photosynthesis by MWCNTs improved sugar metabolism as indicated by increased activity of sugar metabolic enzymes such as amylase, starch synthase and invertase. This also supplied the carbon necessary for the production of primary (amino acids, fatty acids and organic acids) and secondary (flavonoids and polyphenols) metabolites. There was consistently higher activity of antioxidant enzymes (catalase and peroxidase). Interestingly, species-specific reactions to MWCNT priming were observed, where L. sativum sprouts showed the highest antioxidant activity, followed by A. graveolens., Conclusion: MWCNT priming improves sprout growth and nutritional quality by boosting metabolic processes and antioxidant activity, presenting a promising approach for sustainable agriculture. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)

Desert plants possess untapped potential for medicinal applications due to their rich phytochemical profiles. However, they need to be more explored. Thus, this study integrates advanced analytical, biochemical, and molecular techniques to investigate the phytochemical composition and biological activities (antimicrobial and antioxidant) of four desert plants ( Pergularia tomentosa, Zygophyllum coccineum, Pulicaria undulata , and Ochradenus baccatus) , collected from Wadi Sannor, Beni-Suef Governorate, Egypt, in March 2021. The volatile chemicals in the 70 % ethanol extracts of the selected plants were also analyzed using GC-MS. The extract exhibited strong antioxidant properties, as demonstrated by its FRAP (Ferric reducing ability of plasma) values, anti-lipid peroxidation, superoxide anion scavenging activity, and DPPH scavenging activity. Additionally, plants extracts showed high antimicrobial activities against seven pathogens, including three Gram-negative bacteria ( Pseudomonas aeruginosa , Salmonella typhimurium , Escherichia coli ) and four Gram-positive bacteria (Staphylococcus saprophyticus, Staphylococcus epidermidis, Enterococcus faecalis , Streptococcus salivarius). Lastly, molecular docking was conducted for cis-vaccenic acid, (E)-9-octadecenoic acid, the cyclohepta[b]furan-2-one scaffold, and URS-20(30)-en-3-ol against both the thymidylate kinase enzyme and the active sites of E. coli DNA gyrase. The results from the molecular docking studies showed a strong correlation with the biological data. Moreover, these compounds exhibited good, proposed absorption, distribution, metabolism, and excretion-toxicity (ADMET) profiles. Our study highlights the potential of P. tomentosa, Z. coccineum, P. undulata, and O. baccatus for future medical applications and the development of new pharmaceuticals derived from desert flora., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

The ecological construction in coastal saline-alkali areas urgently needs to be explored in terms of water-salt regulation management and functional irrigation. In this paper, the extremely severe saline soil in the eastern coastal area of the North China Plain was selected for vegetation rehabilitation by drip irrigation. Through two-year field experiments, the spatial and temporal soil salinity-water dynamics of three land use types (LUTS) under two irrigation strategies (IIS) were systematically studied. The results revealed: (1) The soils in the understory grassland and shrub land root zones remained stable for desalination, with the average ECe decreasing to 0.69 dS/m and 0.71 dS/m under autumn irrigation at surface, and 0.66 dS/m and 0.85 dS/m under winter irrigation. And a slight salt accumulation occurred in the bare land in stage IV. (2)The soil surface moisture increased, and the bulk density decreased significantly with drip irrigation. The final moisture of understory grassland and shrub land was 3.85 and 2.97 times that of the bare land layer at 0-10 cm, 2.55 and 1.97 times at 10-20 cm, and 1.61 times and 1.47 times at 20-40 cm, respectively. (3)Due to quick salt rinsing, Salix matsudana and Hibiscus maintained a high survival rate, and the germination of understory vegetation further increased the vegetation coverage. Meanwhile perennial understory herbs gradually became the dominant species, which positively effects on the maintenance of soil low-salt environment. (4)There were significant differences in SWC and ECe between autumn and winter irrigation treatment during stage I and stage II, indicating that irrigation strategies only impact on soil water-salt movement in the early stage. While there was no significant difference between understory grassland and shrub land, indicating that the research about the effects of vegetation cover type on water-salt transport should take a longer time scale., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Plants are subjected to various biotic and abiotic stresses that significantly impact their growth and productivity. To achieve balanced crop growth and yield, including for leafy vegetables, the continuous application of micronutrient is crucial. This study investigates the effects of different concentrations of copper sulphate (0, 75, 125, and 175 ppm) on the morphological and biochemical features of Spinacia oleracea and Avena sativa. Morphological parameters such as plant height, leaf area, root length, and fresh and dry weights were optimized at a concentration of 75 ppm copper sulfate. At this concentration, chlorophyll a & b levels increased significantly in Spinacia oleracea (462.9 and 249.8 𝜇𝑔/𝑔), and Avena sativa (404.7 and 437.63𝜇𝑔/𝑔). However, carotenoid content and sugar levels in Spinacia oleracea were negatively affected, while sugar content in Avena sativa increased at 125 ppm (941.6 µg/ml). Protein content increased in Spinacia oleracea (75 ppm, 180.3 µg/ml) but decreased in Avena sativa. Phenol content peaked in both plants at 75 ppm (362.2 and 244.5 µg/ml). Higher concentrations (175 ppm) of copper sulfate reduced plant productivity and health. Plants exposed to control and optimal concentrations (75 and 125 ppm) of copper sulpate exhibited the best health and growth compared to those subjected to higher concentrations. Maximum plant height, leaf area, root length, fresh and dry weights were observed at lower concentrations (75 and 125 ppm) of copper sulfate, while higher concentrations caused toxicity. Optimal copper sulfate levels enhanced chlorophyll a, chlorophyll b, total chlorophyll, protein, and phenol contents but inhibited sugar and carotenoid contents in both Spinacia oleracea and Avena sativa. Overall, increased copper sulfate treatment adversely affected the growth parameters and biochemical profiles of these plants., (© 2024. The Author(s).)

Background: Gadolinium (Gd) is an increasingly found lanthanide element in soil; thus, understanding its impact on plant physiology, biochemistry, and molecular responses is crucial. Here, we aimed to provide a comprehensive understanding of Gd (150 mg kg - 1 ) impacts on guar (Cyamopsis tetragonoloba L.) plant yield and metabolism and whether the symbiotic relationship with arbuscular mycorrhizal fungi (AMF) can mitigate Gd toxicity of soil contamination., Results: AMF treatment improved mineral nutrient uptake and seed yield by 38-41% under Gd stress compared to non-inoculated stressed plants. Metabolic analysis unveiled the defense mechanisms adopted by AMF-treated plants, revealing carbon and nitrogen metabolism adaptations to withstand Gd contamination. This included an increase in the synthesis of primary metabolites, such as total sugar (+ 39% compared to control), soluble sugars (+ 29%), starch (+ 30%), and some main amino acids like proline (+ 57%) and phenylalanine (+ 87%) in the seeds of AMF-treated plants grown under Gd contamination. Furthermore, fatty acid and organic acid profile changes were accompanied by the production of secondary metabolites, including tocopherols, polyamines, phenolic acids, flavones, and anthocyanins., Conclusions: Overall, the coordinated synthesis of these compounds underscores the intricate regulatory mechanisms underlying plant-AMF interactions and highlights the potential of AMF to modulate plant secondary metabolism for enhanced Gd stress tolerance., (© 2024. The Author(s).)

The aim of the present research was to evaluate the efficiency of different vegetation indices (VI) obtained from satellites with varying spatial resolutions in discriminating the phenological stages of soybean crops. The experiment was carried out in a soybean cultivation area irrigated by central pivot, in Balsas, MA, Brazil, where weekly assessments of phenology and leaf area index were carried out. Throughout the crop cycle, spectral data from the study area were collected from sensors, onboard the Sentinel-2 and Amazônia-1 satellites. The images obtained were processed to obtain the VI based on NIR (NDVI, NDWI and SAVI) and RGB (VARI, IV GREEN and GLI), for the different phenological stages of the crop. The efficiency in identifying phenological stages by VI was determined through discriminant analysis and the Algorithm Neural Network-ANN, where the best classifications presented an Apparent Error Rate (APER) equal to zero. The APER for the discriminant analysis varied between 53.4% and 70.4% while, for the ANN, it was between 47.4% and 73.9%, making it not possible to identify which of the two analysis techniques is more appropriate. The study results demonstrated that the difference in sensors spatial resolution is not a determining factor in the correct identification of soybean phenological stages. Although no VI, obtained from the Amazônia-1 and Sentinel-2 sensor systems, was 100% effective in identifying all phenological stages, specific indices can be used to identify some key phenological stages of soybean crops, such as: flowering (R1 and R2); pod development (R4); grain development (R5.1); and plant physiological maturity (R8). Therefore, VI obtained from orbital sensors are effective in identifying soybean phenological stages quickly and cheaply., Competing Interests: NO authors have competing interests., (Copyright: © 2024 da Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Plant growth-promoting bacteria (PGPB) are considered a promising tool for triggering the synthesis of bioactive compounds in plants and to produce healthy foods. This study aimed to demonstrate the impact of PGPB on the growth, accumulation of primary and secondary metabolites, biological activities, and nutritional qualities of Eruca sativa (arugula), a key leafy vegetable worldwide. To this end, Jeotgalicoccus sp. (JW0823), was isolated and identified by using partial 16S rDNA-based identification and phylogenetic analysis. The findings revealed that JW0823 significantly boosted plant biomass production by about 45% (P<0.05) and enhanced pigment contents by 47.5% to 83.8%. JW0823-treated plants showed remarkable improvements in their proximate composition and vitamin contents, with vitamin E levels increasing by 161.5%. JW0823 induced the accumulation of bioactive metabolites including antioxidants, vitamins, unsaturated fatty acids, and essential amino acids, thereby improving the nutritional qualities of treated plants. An increase in the amounts of amino acids was recorded, with isoleucine showing the highest increase of 270.2%. This was accompanied by increased activity of the key enzymes involved in amino acid biosynthesis, including glutamine synthase, dihydrodipicolinate synthase, cystathionine γ-synthase, and phenylalanine ammonia-lyase enzymes. Consequently, the total antioxidant and antidiabetic activities of the inoculated plants were enhanced. Additionally, JW0823 improved antimicrobial activity against several pathogenic microorganisms. Overall, the JW0823 treatment is a highly promising method for enhancing the health-promoting properties and biological characteristics of E. sativa, making it a valuable tool for improving the quality of this important leafy vegetable., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Hassan, Maridueña-Zavala, Alsherif, Aloufi, Korany, Aldilami, Bouqellah, Reyad and AbdElgawad.)

Wheat (Triticum aestivum L.) is a staple food crop that plays a crucial role in global food security. A suitable planting pattern and optimum nitrogen (N) split management are efficient practices for improving wheat production. Therefore, an experiment was performed to explore the effect of N split management and sowing patterns on wheat at the Agronomy Research Farm, The University of Agriculture Peshawar, during rabi season 2020-21 and 2021-22. The treatments consisted of different nitrogen rates of 0, 80, 120, and 160 kg ha - 1 and planting patterns of W, M, broadcast and line sowing. The pooled analysis of both cropping seasons showed that application of 120 kg N ha - 1 increased spikelets spike - 1 , grains spike - 1 , 1000 grains weight, grain yield, grain N content, evapotranspiration and water use efficiency by 21.9, 16.7, 21.8, 70, 13, 19.9 and 40% as compared to control, respectively. In addition, W and M were observed the best management practices among all planting patterns. The M planting pattern enhanced chlorophyll a, b, carotenoids and evapotranspiration while W plating pattern improved yield components and yield of wheat as compared to broadcast planting patterns. The principal component analysis biplot showed a close association of M and W planting patterns with 120 kg N ha - 1 in most of the studied traits. Hence, it is concluded that split application of 120 kg N ha - 1 in W and M sowing patterns enhanced growth, biochemical traits and water use efficiency, reducing N fertilization from 160 to 120 kg ha - 1 while increasing grain yield of wheat. Hence, it is recommended that application of 120 kg N ha⁻¹ in combination with W and M planting patterns offer a sustainable approach to enhancing wheat production in the alkaline soil conditions of the Peshawar valley., (© 2024. The Author(s).)

Layer double hydroxide (LDH) nanoparticles (NPs) have been applied to enhance plant growth and productivity. However, their effects on carbon and nitrogen metabolism of aromatic plants, are not well understood. Therefore, we investigated the impact of foliar application of Zn-Al LDH and Mg-Al LDH NPs (10 ppm) on the growth and metabolism of geranium plants. Zn-Al LDH and Mg-Al LDH NPs significantly increased the dry biomass, photosynthetic pigment, and Zn and Mg uptake by treated plants. These increases were consistent with increased primary metabolism such as soluble sugars and their metabolic enzymes (invertase and amylase). The supply of high sugar levels induced TCA organic accumulation, providing a pathway for amino acid biosynthesis. Among amino acids, proline level and its biosynthetic enzymes such as pyrroline-5-carboxylate reductase (P5CR), ornithine aminotransferase (OAT), and pyrroline-5-carboxylate synthetase (P5CS), glutamine synthetase (GS), and arginase were increased. Increased primary metabolites can then be channeled into secondary metabolic pathways, leading to higher levels of secondary metabolites including tocopherols, phenolics, and flavonoids. These observed increases in primary and secondary metabolites also improve the biological value of geranium plants. Overall, our research highlights the potential of Zn-Al LDH and Mg-Al LDH NPs as elicitors to enhance metabolism in geranium plants, thereby improving their growth bioactivity., Competing Interests: All authors declare that they have no competing interests., (This journal is © The Royal Society of Chemistry.)

This study investigates the differential responses of two maize genotypes, SC180 and SC168, to salt stress, aiming to elucidate the mechanisms underlying salinity tolerance and identify traits associated with improved stress resilience. Salinity stress, imposed by 150 mM NaCl, adversely affected various growth parameters in both genotypes. SC180 exhibited a more pronounced reduction in shoot length (13.6%) and root length (13.6%) compared to SC168, which showed minimal reductions (3.0% and 2.3%, respectively). Additionally, dry weight losses in SC180's leaves, stems, and roots were significantly greater than those in SC168. Under salinity stress, both genotypes accumulated Na + in all organs, with SC168 showing higher Na + concentrations. However, K + levels decreased more significantly in SC180's leaves than in SC168's. The study also assessed physiological responses, noting that SC180 experienced a substantial reduction in relative water content (RWC) in leaves (22.7%), while SC168's RWC remained relatively stable (5.15%). Proline accumulation, a marker for osmotic adjustment, increased 2.3-fold in SC168 compared onefold in SC180. Oxidative stress indicators, such as electrolyte leakage and hydrogen peroxide levels, were elevated in both genotypes under salt stress, with SC180 showing higher increases (48.5% and 48.7%, respectively) than SC168 (35.25% and 22.0%). Moreover, antioxidant enzymes (APX, CAT, POD, SOD, GR) activities were significantly enhanced in SC168 under salinity stress, whereas SC180 showed no significant changes in these activities. Stress indices, used to quantify and compare salinity tolerance, consistently ranked SC168 as more tolerant (average rank = 1.08) compared to SC180 (average rank = 1.92). Correlation analyses further confirmed that SC168's superior tolerance was associated with better Na + regulation, maintenance of K + levels, and a robust antioxidant defense system. In conclusion, SC168 demonstrated greater resilience to salinity stress, attributed to its efficient ion regulation, stable water status, enhanced osmotic adjustment, and strong antioxidant response. These findings provide valuable insights for breeding and developing salinity-tolerant maize varieties., (© 2024. The Author(s).)

Introduction: Plant growth-promoting bacteria (PGPB) have been primarily studied for atmospheric nitrogen (N) fixation but they also have the capacity to improve nutrition and yield of crop plants., Methods: Therefore, the objective of this research was to investigate the effects of inoculation with PGPB in association with different N rates on N uptake, grain yield, and oil concentration of dwarf castor beans in succession to legumes and grasses in Ilha Solteira, Brazil. The treatments consisted of N rates (0 to 180 kg ha -1 of N) and inoculation with three plant growth-promoting bacteria ( Azospirillum brasiliense, Bacillus subtilis , and Pseudomonas fluorescens , applied by leaf) and a control with no-inoculation., Results: The grain and oil yields of castor beans were increased by 20 and 40% at a rate of 103 kg ha -1 of N in succession to grasses as compared to without N application. In addition, the grain yield of castor bean after legumes was increased by 28, 64, and 40% with estimated rates of 97, 113, and 92 kg ha -1 of N in combination with inoculations of A. brasilense , B. subtilis , and P. fluorescens as compared to without N application, respectively. Shoot, grain, and total N uptake were improved with foliar inoculation of A. brasilense , B. subtilis , and P. fluorescens at the N rates of 45, 90, and 135 kg ha -1 , respectively., Discussion and Conclusions: Topdressing of N at the rate of 103 kg ha -1 and foliar inoculation in succession to grasses and 180 kg ha -1 of N without the effect of foliar inoculation in succession to legumes are recommended for higher grain and oil yield of castor beans. Foliar inoculations with A. brasilense , B. subtilis , and P. fluorescens increased grain yield under reduced use of N fertilizer by 44, 37, and 49% in dwarf castor cultivation in succession to legumes, potentially contributing to sustainable agriculture., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Gato, Oliveira, Jalal, Moreira, Hashem, de Lima, Leite, Al-Askar, Freitas, AbdElgawad, Ferreira, Santana, Bastos, Galindo, Zoz and Teixeira Filho.)

Introduction: Salinity negatively affects maize productivity. However, calcium lignosulfonate (CLS) could improve soil properties and maize productivity., Methods: In this study, we evaluated the effects of CLS application on soil chemical properties, plant physiology and grain quality of maize under salinity stress. Thus, this experiment was conducted using three CLS application rates, CLS 0 , CLS 5 , and CLS 10 , corresponding to 0%, 5%, and 10% of soil mass, for three irrigation water salinity (WS) levels WS 0.5 , WS 2.5 , and WS 5.5 corresponding to 0.5 and 2.5 and 5.5 dS/m, respectively., Results and Discussion: Results show that the WS 0.5 × CLS 10 combination increased potassium (K 0.167 g/kg), and calcium (Ca, 0.39 g/kg) values while reducing the sodium (Na, 0.23 g/kg) content in soil. However, the treatment WS 5.5 × CLS 0 decreased K (0.120 g/kg), and Ca (0.15 g/kg) values while increasing Na (0.75 g/kg) content in soil. The root activity was larger in WS 0.5 × CLS 10 than in WS 5.5 × CLS 0 , as the former combination enlarged K and Ca contents in the root while the latter decreased their values. The leaf glutamine synthetase (953.9 µmol/(g.h)) and nitrate reductase (40.39 µg/(g.h)) were higher in WS 0.5 × CLS 10 than in WS 5.5 × CLS 0 at 573.4 µmol/(g.h) and 20.76 µg/(g.h), leading to the improvement in cell progression cycle, as revealed by lower malonaldehyde level (6.57 µmol/g). The K and Ca contents in the leaf (881, 278 mg/plant), stem (1314, 731 mg/plant), and grains (1330, 1117 mg/plant) were greater in WS 0.5 × CLS 10 than in WS 5.5 × CLS 0 at (146, 21 mg/plant), (201, 159 mg/plant) and (206, 157 mg/plant), respectively. Therefore, the maize was more resistance to salt stress under the CLS 10 level, as a 7.34% decline in yield was noticed when salinity surpassed the threshold value (5.96 dS/m). The protein (13.6 %) and starch (89.2 %) contents were greater in WS 0.5 × CLS 10 than in WS 5.5 × CLS 0 (6.1 %) and (67.0 %), respectively. This study reveals that CLS addition can alleviate the adverse impacts of salinity on soil quality and maize productivity. Thus, CLS application could be used as an effective soil amendment when irrigating with saline water for sustainable maize production., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Alhaj Hamoud, Shaghaleh, Zhang, Okla, Alaraidh, AbdElgawad and Sheteiwy.)

To reduce heavy metal toxicity, like that induced by thallium (TI) in plants, growth-promoting bacteria (GPB) are a widely used to enhance plant tolerance to heavy metals toxicity. In our study, we characterized seven GPB and identified Actinoplanes spp., as the most active strain. This bioactive strain was then applied to alleviate TI phytotoxicity. TI contamination (20 mg/kg soil) induced TI bioaccumulation, reducing wheat growth (biomass accumulation) and photosynthesis rate, by about 55% and 90%, respectively. TI stress also induced oxidative damages as indicated by increased oxidative markers (H 2 O 2 and lipid peroxidation (MDA)). Interestingly, Actinoplanes spp. significantly reduced growth inhibition and oxidative stress by 20% and 70%, respectively. As a defense mechanism to mitigate the TI toxicity, wheat plants showed improved antioxidant and detoxification defense including increased phenolic and tocopherols levels as well as peroxidase (POX), catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR) enzymes activities. These defense mechanisms were further induced by Actinoplanes spp. Additionally, Actinoplanes spp. increased the production of heavy metal-binding ligands such as metallothionein, phytochelatins, total glutathione, and glutathione S-transferase activity by 100%, 90%, 120%, and 100%, respectively. This study, therefore, elucidated the physiological and biochemical bases underlying TI-stress mitigation impact of Actinoplanes spp. Overall, Actinoplanes spp. holds promise as a valuable approach for ameliorating TI toxicity in plants. KEYBOARD: Actinobacteria, Bioaccumulation, Detoxification, Membrane damage, Redox regulation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Rice (Oryza sativa L.) is an essential food for half of the global population and is vital in maintaining global food security. Climate change, increasing population and recent incident of COVID pandemic has generated financial burden and threaten the global food security. Due to theses factors rice cultivation also has to face significant challenges. frequent weather changes pose a considerable challenge to agricultural planning, which was previously relaying on consistent seasonal variations. In this context, rice cultivation is particularly sensitive to cold, where its development and productivity inhibited by low temperatures (< 18 °C). Developing rice varietes with low temprature tolerence and good yield potential is one of the major goals of current breeding efforts of plant scientists. For this purpose, short duration and early rice varieties are most favorable to avoid cold stress and yield more in less number of days. this study was designed to investigate the effect of low temperatures on different rice varieties. the study was designed to identify low temprature tolerent genotypes with early and regular cultivation. For this, thirty-four genotypes were evaluated in two gorwing seasons (2018-2019) with four different sowing times. Statistically sowing time showed significant interaction between all yield contributing parameters. The data indicate that exposure to low temperatures during the reproductive phase prolongs the maturation period of the crop, also length of the panicle and the fertility of the spikelets drops, resulting in a significant decrease in the production of sensitive varieties. Some varieties are more sensitive to cold stress compared to others. In the Egyptian context, Giza176, Sakha104, and Sakha107 are recommended for early cultivation, while the genotypes Giza 179, Sakha101, Sakha104, and GZ 9730-1-1-1-1 are indicated for the normal cultivation period. The Sakha104 variety is particularly notable, as it is recommended for both purposes. In addition, the data obtained in this study provide valuable information for selecting rice varieties suitable for double cropping in the North Delta of Egypt. This study also contributes to the existing literature, providing insights into the resilience of rice cultivation in the face of climate change., (© 2024. The Author(s).)

Anise ( Pimpinella anisum L.) seeds have various nutritional and therapeutic benefits and are thus considered a valuable addition to animal and human health. Hence, in this study, we aimed to induce the nutritive and biological value of anise seeds. To this end, the potential biofortification effect of the endophytic Actinomycetota sp. JW0824 strain, isolated during the fall of 2023 from the medicinal plant Achyranthes aspera , exhibiting natural distribution in the Jazan region of Saudi Arabia, was investigated in four varieties of anise seeds from Egypt, Tunisia, Syria, and Morocco. Results revealed significant increments ( p < 0.05) in the seed dry weight percentage (DW%) and oil yields. In line with increased biomass accumulation, the metabolism of the primary and secondary metabolites was increased. There were differential increases in proteins, sugars, flavonoids, alkaloids, phenols, vitamins (e.g., β-carotene, ascorbic acid), and essential oil components (e.g., phenylpropanoids and monoterpenes), along with their precursor phenylalanine. Consistently, the activity of L-phenylalanine aminolyase (PAL) was increased in the Egyptian and Tunisian varieties at 83.88% and 77.19%, respectively, while 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) activity increased in all varieties, with a significant 179.31% rise in the Egyptian variety. These findings highlight the beneficial effects of Actinomycetota sp. JW0824 as a bioinoculant for anise seeds, suggesting its potential application in agricultural practices to improve seed yield and quality. Further field trials are recommended to assess the commercial viability of this endophyte for enhancing anise seed production and potentially benefiting other plant species.

Background: Coffee farming constitutes a substantial economic resource, representing a source of income for several countries due to the high consumption of coffee worldwide. Precise management of coffee crops involves collecting crop attributes (characteristics of the soil and the plant), mapping, and applying inputs according to the plants' needs. This differentiated management is precision coffee growing and it stands out for its increased yield and sustainability., Results: This research aimed to predict yield in coffee plantations by applying machine learning methodologies to soil and plant attributes. The data were obtained in a field of 54.6 ha during two consecutive seasons, applying varied fertilization rates in accordance with the recommendations of soil attribute maps. Leaf analysis maps also were monitored with the aim of establishing a correlation between input parameters and yield prediction. The machine-learning models obtained from these data predicted coffee yield efficiently. The best model demonstrated predictive fit results with a Pearson correlation of 0.86. Soil chemical attributes did not interfere with the prediction models, indicating that this analysis can be dispensed with when applying these models., Conclusion: These findings have important implications for optimizing coffee management and cultivation, providing valuable insights for producers and researchers interested in maximizing yield using precision agriculture. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)

Background: Atrial functional mitral regurgitation (AFMR) is best described with normal left ventricular size and function, structurally normal mitral leaflets and dilated left atrium. Unlike the ventricular functional phenotype, changes in the annular geometry more than the tethering forces are the main culprit for mitral regurgitation. The aim of this study is to illuminate the leaflet-annular remodeling in patients with mitral regurgitation and atrial fibrillation (AF) using three-dimensional transesophageal echocardiography (3D TOE)., Results: Consecutive fifty patients with AFMR underwent transthoracic echocardiography and 3D TOE: 25 patients with AF and non-mild MR and 25 patients with AF and mild MR were studied. A special mitral valve analysis software was used to accurately assess the three unique pillars for MR: annular size, leaflets' geometry and tenting parameters. Compared to the mild MR group, non-mild MR group had long-standing AF of more than 1 year and larger left atrial volumes (51.83 ± 12.07 ml/m 2 vs 33.68 ± 10.97 ml/m 2 , p < 0.001). No significant differences were noted in respect of tenting height, area and volume (13.06 ± 2.57 mm vs 11.43 ± 2.89 mm, p = 0.064, 3.58 ± 1.26 cm 2 vs 2.80 ± 0.95 cm 2 , 0.081 and 6.70 ± 2.96 cm 3 vs 5.04 ± 2.32 cm 3 , p = 0.081). Conversely, the non-mild MR group had larger annular area and perimeter (16.20 ± 3.90 cm 2 vs 13.51 ± 3.85 cm 2 , p = 0.023 and 14.73 ± 1.72 cm vs 13.46 ± 1.79 cm, p = 0.033). Similarly, the non-mild MR group had larger anterior and posterior leaflets' areas (10.18 ± 4.02 cm 2 vs 8.71 ± 3.08 cm 2 , p = 0.04 and 8.96 ± 2.60 cm 2 vs 7.30 ± 2.17 cm 2 , p = 0.029). Correspondingly, more disproportionate leaflet-annular remodeling, as assessed by the ratio of total leaflets' area to the annular area, was noted in the non-mild MR as opposed to the mild MR group (1.22 ± 0.04 vs 1.26 ± 0.04, p = 0.008)., Conclusions: Recently, AFMR has been recognized as a remarkable entity of secondary MR with unique mechanisms. Annular dilatation with disproportionate leaflet remodeling can validate the central regurgitation. However, the call for more parameters is being emphasized to characterize the suitable candidates for percutaneous interventions., (© 2024. Crown.)