25 results on '"Abdelmajid Noubhani"'
Search Results
2. A Recombinant Human Anti-Platelet scFv Antibody Produced in Pichia pastoris for Atheroma Targeting.
- Author
-
Amelie Vallet-Courbin, Mélusine Larivière, Agnès Hocquellet, Audrey Hemadou, Sarjapura-Nagaraja Parimala, Jeanny Laroche-Traineau, Xavier Santarelli, Gisèle Clofent-Sanchez, Marie-Josée Jacobin-Valat, and Abdelmajid Noubhani
- Subjects
Medicine ,Science - Abstract
Cells of the innate and adaptive immune system are key factors in the progression of atherosclerotic plaque, leading to plaque instability and rupture, potentially resulting in acute atherothrombotic events such as coronary artery disease, cerebrovascular disease and peripheral arterial disease. Here, we describe the cloning, expression, purification, and immunoreactivity assessment of a recombinant single-chain variable fragment (scFv) derived from a human anti-αIIbβ3 antibody (HuAb) selected to target atheromatous lesions for the presence of platelets. Indeed, platelets within atheroma plaques have been shown to play a role in inflammation, in platelet-leucocyte aggregates and in thrombi formation and might thus be considered relevant biomarkers of atherosclerotic progression. The DNA sequence that encodes the anti-αIIbβ3 TEG4 scFv previously obtained from a phage-display selection on activated platelets, was inserted into the eukaryote vector (pPICZαA) in fusion with a tag sequence encoding 2 cysteines useable for specific probes grafting experiments. The recombinant protein was expressed at high yields in Pichia pastoris (30 mg/L culture). The advantage of P. pastoris as an expression system is the production and secretion of recombinant proteins in the supernatant, ruling out the difficulties encountered when scFv are produced in the cytoplasm of bacteria (low yield, low solubility and reduced affinity). The improved conditions allowed for the recovery of highly purified and biologically active scFv fragments ready to be grafted in a site-directed way to nanoparticles for the imaging of atherosclerotic plaques involving inflammatory processes and thus at high risk of instability.
- Published
- 2017
- Full Text
- View/download PDF
3. Structural and molecular basis of cross-seeding barriers in amyloids
- Author
-
Denis Martinez, Abdelmajid Noubhani, Loren B. Andreas, Guido Pintacuda, Benjamin Bardiaux, Brice Kauffmann, Asen Daskalov, Mélanie Berbon, Antoine Loquet, Joseph S. Wall, Virginie Coustou, Nadia El Mammeri, Jan Stanek, Sven J. Saupe, Birgit Habenstein, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Centre de Résonance Magnetique Nucleaire (CRMN), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Bioinformatique structurale - Structural Bioinformatics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Européen de Chimie et Biologie (IECB), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Brookhaven National Laboratory [Upton, NY] (BNL), UT-Battelle, LLC-Stony Brook University [SUNY] (SBU), State University of New York (SUNY)-State University of New York (SUNY)-U.S. Department of Energy [Washington] (DOE), We acknowledge financial support from the European Research Council (ERC) under the European Unions Horizon 2020 Research and Innovation Programme (ERC-2015-CoG GA 648974 to G.P. and ERC-2015-StG GA 639020 to A.L.), IdEx Bordeaux (Chaire d’Installation to B.H., ANR-10-IDEX-03-02), the Agence Nationale de la Recherche (ANR) (ANR-14-CE09-0020-01 to A.L., ANR-13-PDOC-0017-01 to B.H. and ANR-17-CE11-0035 to S.J.S), the INCEPTION project (Programme d'investissements d'avenir/ANR-16-CONV-0005) and the CNRS (IR-RMN FR3050). J.S. and L.B.A. were supported by individual Marie Sklodowska-Curie incoming fellowships (grant agreements 661799 'COMPLEX-FAST-MAS' and 624918 'MEM-MAS'). A.D. was supported by the Nouvelle Aquitaine Regional Council., We thank the Nouvelle Aquitaine Regional Council, University of Bordeaux, and the Contrat Plan Etat-Region (CPER) CampusB Bordeaux for the acquisition of the NMR equipment. This work has benefited from the Biophysical and Structural Chemistry Platform at IECB, CNRS UMS 3033, INSERM US001., ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), ANR-14-CE09-0020,NanoSSNMR,Nanostructures biologiques et synthétiques étudiées par Résonance Magnétique Nucléaire du Solide(2014), ANR-13-PDOC-0017,SUPRAMOL,Structures d'Assemblages Supramoléculaires par RMN du Solide : le Pseudopilus du Système de Sécrétion de Type II et le Tube de Queue du Bactériophage(2013), ANR-17-CE11-0035,SFAS,Structure et fonctions de motifs amyloïdes impliqués dans la transduction du signal(2017), ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016), European Project: 648974,H2020,ERC-2014-CoG,P-MEM-NMR(2015), European Project: 639020,H2020,ERC-2014-STG,Weakinteract(2015), European Project: 661799,H2020,H2020-MSCA-IF-2014,COMPLEX-fastMAS-NMR(2016), European Project: 624918,EC:FP7:PEOPLE,FP7-PEOPLE-2013-IIF,MEM-MAS(2014), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), U.S. Department of Energy [Washington] (DOE)-UT-Battelle, LLC-Stony Brook University [SUNY] (SBU), and State University of New York (SUNY)-State University of New York (SUNY)
- Subjects
0301 basic medicine ,Protein Folding ,Amyloid ,Prions ,Prion strain ,Model system ,Amyloidogenic Proteins ,Computational biology ,Biology ,010402 general chemistry ,01 natural sciences ,Models, Biological ,Podospora anserina ,Fungal Proteins ,prion ,03 medical and health sciences ,Protein Aggregates ,0302 clinical medicine ,Podospora ,Amino Acid Sequence ,Prion protein ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Conserved Sequence ,030304 developmental biology ,cross-seeding ,0303 health sciences ,Multidisciplinary ,food and beverages ,amyloid ,Biological Sciences ,biology.organism_classification ,Sequence identity ,0104 chemical sciences ,Protein Structure, Tertiary ,[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,Strain specificity ,nuclear magnetic resonance ,030104 developmental biology ,sequence to fold ,Sequence Alignment ,030217 neurology & neurosurgery - Abstract
Neurodegenerative disorders are frequently associated with β-sheet-rich amyloid deposits. Amyloid-forming proteins can aggregate under different structural conformations known as strains, which can exhibit a prion-like behaviour and distinct patho-phenotypes. Precise molecular determinants defining strain specificity and cross-strain interactions (cross-seeding) are currently unknown. The HET-s prion protein from the fungus Podospora anserina represents a model system to study the fundamental properties of prion amyloids. Here, we report the amyloid prion structure of HELLF, a distant homolog of the model prion HET-s. We find that these two amyloids, sharing only 17% sequence identity, have nearly identical β-solenoid folds but lack cross-seeding ability in vivo, indicating that prion specificity can differ in extremely similar amyloid folds. We engineer the HELLF sequence to explore the limits of the sequence-to-fold conservation and to pinpoint determinants of cross-seeding and prion specificity. We find that amyloid fold conservation occurs even at an exceedingly low level of identity to HET-s (5%). Next, we derive a HELLF-based sequence, termed HEC, able to breach the cross-seeding barrier in vivo between HELLF and HET-s, unveiling determinants controlling cross-seeding at residue level. These findings show that virtually identical amyloid backbone structures might not be sufficient for cross-seeding and that critical side-chain positions could determine the seeding specificity of an amyloid fold. Our work redefines the conceptual boundaries of prion strain and shed new light on key molecular features concerning an important class of pathogenic agents.
- Published
- 2021
4. Zuordnung der Rückgrat- und Seitenketten-Protonen in vollständig protonierten Proteinen durch Festkörper-NMR-Spektroskopie: Mikrokristalle, Sedimente und Amyloidfibrillen
- Author
-
Kaspars Tars, Serena Leone, Nicholas E. Dixon, Delia Picone, Svetlana Kotelovica, Mélanie Berbon, Birgit Habenstein, Sven J. Saupe, Zhi-Qiang Xu, Andrea Bertarello, Antoine Loquet, Denis Martinez, Jan Stanek, Abdelmajid Noubhani, Diane Cala, Andrea Pica, Inara Akopjana, Kristaps Jaudzems, Loren B. Andreas, Daniela Lalli, Guido Pintacuda, Tobias Schubeis, and Nadia El Mammeri
- Subjects
010405 organic chemistry ,Chemistry ,General Medicine ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences - Published
- 2016
5. Multimodal molecular imaging of atherosclerosis: Nanoparticles functionalized with scFv fragments of an anti-αIIbβ3 antibody
- Author
-
Stéphane Mornet, Jeanny Laroche-Traineau, Carmelo Di Primo, Laetitia Minder, Xavier Santarelli, Gisèle Clofent-Sanchez, Mélusine Larivière, Stéphane Sanchez, Samuel Bonnet, Cyril Lorenzato, Laurent Adumeau, Audrey Hemadou, Marie-Josée Jacobin-Valat, Abdelmajid Noubhani, Centre de résonance magnétique des systèmes biologiques (CRMSB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut Bergonié [Bordeaux], UNICANCER, Régulations Naturelles et Artificielles (ARNA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bordeaux Ségalen [Bordeaux 2], Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Européen de Chimie et de Biologie, Bordeaux - Recherche Intégrée en Oncologie (SIRIC-BRIO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), This work has benefited from the Vivoptic imaging platform supported by France Life Imaging. More particularly, we would like to thank C. Genevois for her technical support. This work has benefited from the facilities and expertise of the Biophysical and Structural Chemistry plateform (BPCS) at IECB, CNRS UMS3033, Inserm US001, Bordeaux University http://www.iecb.u-bordeaux.fr/index.php/fr/plateformestechnologiques. The microscopy was done in the Bordeaux Imaging Center a service unit of the CNRS-INSERM and Bordeaux University, member of the national infrastructure France BioImaging supported by the French National Research Agency (ANR-10-INBS-04). The help of Sébastien Marais is acknowledged. We would like to thank the Haut-Lévèque Hospital in Pessac, France, for providing the human biological material., ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), and Admin, Oskar
- Subjects
Male ,Integrin ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Near infrared fluorescence (NIRF) ,Bioengineering ,02 engineering and technology ,Platelet Glycoprotein GPIIb-IIIa Complex ,Multimodal Imaging ,Fluorescence ,03 medical and health sciences ,Mice ,In vivo ,medicine ,Bimodal imaging ,Animals ,General Materials Science ,Avidity ,Tissue Distribution ,Platelet activation ,030304 developmental biology ,[CHIM.MATE] Chemical Sciences/Material chemistry ,0303 health sciences ,biology ,Chemistry ,Directed conjugation ,[CHIM.MATE]Chemical Sciences/Material chemistry ,021001 nanoscience & nanotechnology ,medicine.disease ,Atherosclerosis ,scFv antibody fragments ,Magnetic Resonance Imaging ,3. Good health ,Molecular Imaging ,Atheroma ,SPIO ,biology.protein ,Biophysics ,Molecular Medicine ,Nanoparticles ,Rabbits ,Antibody ,Molecular imaging ,0210 nano-technology ,Ex vivo ,Single-Chain Antibodies - Abstract
International audience; Due to the wealth of actors involved in the development of atherosclerosis, molecular imaging based on the targeting of specific markers would substantiate the diagnosis of life-threatening atheroma plaques. To this end, TEG4 antibody is a promising candidate targeting the activated platelets (integrin αIIbβ3) highly represented within the plaque. In this study, scFv antibody fragments were used to functionalize multimodal imaging nanoparticles. This grafting was performed in a regio-selective way to preserve TEG4 activity and the avidity of the nanoparticles was studied with respect to the number of grafted antibodies. Subsequently, taking advantage of the nanoparticle bimodality, both near infrared fluorescence and magnetic resonance imaging of the atheroma plaque were performed in the ApoE−/− mouse model. Here we describe the design of the targeted nanoparticles, and a quantification method for their detection in mice, both ex vivo and in vivo, highlighting their value as a potential diagnosis agent.
- Published
- 2018
6. Lipid based nanocarrier compositions loaded with metal nanoparticles and therapeutic agent
- Author
-
PHILIPPE, BARTHELEMY, KHALID, OUMZIL, GISÈLE, CLOFENT-SANCHEZ, MARIE-JOSÉE, JACOBIN-VALAT, JEANNY, LAROCHE-TRAINEAU, STÉPHANE, MORNET, KAREN, GAUDIN, ABDELMAJID, NOUBHANI, XAVIER-FRANÇOIS, SANTARELLI, Admin, Oskar, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), and École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry ,[CHIM.MATE]Chemical Sciences/Material chemistry - Published
- 2018
7. Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR
- Author
-
Manoj Kumar Pandey, Yusuke Nishiyama, Antoine Loquet, Birgit Habenstein, Mélanie Berbon, Abdelmajid Noubhani, James Tolchard, Sven J. Saupe, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut de biochimie et génétique cellulaires (IBGC), and Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Amyloid ,Proton ,Prions ,Very fast MAS ,Protonation ,010402 general chemistry ,Solid-state NMR ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Amide ,Magic angle spinning ,Side chain ,Nuclear Magnetic Resonance, Biomolecular ,Amyloid fibrils ,Spectroscopy ,Proton detection ,Carbon Isotopes ,Carbon-13 ,Resonance ,[CHIM.MATE]Chemical Sciences/Material chemistry ,0104 chemical sciences ,Crystallography ,030104 developmental biology ,chemistry ,Solid-state nuclear magnetic resonance ,Protein NMR ,Protons - Abstract
We present a new solid-state NMR proton-detected three-dimensional experiment dedicated to the observation of protein proton side chain resonances in nano-liter volumes. The experiment takes advantage of very fast magic angle spinning and double quantum 13C-13C transfer to establish efficient (H)CCH correlations detected on side chain protons. Our approach is demonstrated on the HET-s prion domain in its functional amyloid fibrillar form, fully protonated, with a sample amount of less than 500 g using a MAS frequency of 70kHz. The majority of aliphatic and aromatic side chain protons (70%) are observable, in addition to Halpha resonances, in a single experiment providing a complementary approach to the established proton-detected amide-based multidimensional solid-state NMR experiments for the study and resonance assignment of biosolid samples, in particular for aromatic side chain resonances.
- Published
- 2018
8. New marine mammal faunas (Cetacea and Sirenia) and sea level change in the Samlat Formation, Upper Eocene, near Ad-Dakhla in southwestern Morocco
- Author
-
Samir Zouhri, Najia Elboudali, Meriem Rahali, Abdelmajid Noubhani, Samira Sebti, Saïd Meslouh, and Philip D. Gingerich
- Subjects
Eosiren ,Paleontology ,biology ,Marl ,General Engineering ,Saghacetus ,Sirenia ,Dorudon ,biology.organism_classification ,Stromerius ,Siltstone ,Geology ,Archaeoceti - Abstract
The Samlat Formation is well exposed in coastal sections bordering the Atlantic Ocean south of Ad-Dakhla in southwestern Morocco. Here some 22 m of rhythmically-bedded, chert-rich, marine siltstones and marls are overlain by 1–1.5 m of vertebrate-bearing microconglomeratic sandstone, another 4–8 m of rhythmically-bedded siltstone and marl, and finally a second 3–6 m unit of vertebrate-bearing muddy sandstone. The microconglomeratic and muddy sandstones represent low sea stands in what is otherwise a deeper water sequence. Cetacean skeletons are rare but cetacean vertebrae are common in the lower sandstone (bed B1), where many show the effects of reworking. The cetaceans in bed B1represent a minimum of five species, from smallest to largest: cf. Saghacetus sp., cf. Stromerius sp., Dorudon atrox, cf. Dorudon sp., and Basilosaurus isis. Bed B1 yields rib fragments that may represent sirenians, but sirenians, if present, are rare. The only identifiable cetacean found in the upper sandstone (bed B2) is Basilosaurus sp. Dugongid sirenians identified as cf. Eosiren sp. are the most common mammal in bed B2. We interpret co-occurrence of the typically Early Priabonian species Dorudon atrox and Basilosaurus isis with smaller species more like Middle Priabonian genera Saghacetus osiris and Stromerius nidensis to indicate that bed B1 was deposited during low sea stand Pr-2 between the Early and Middle Priabonian (between the early and middle Late Eocene). Bed B2 is separated from B1 by an interval of deeper water sediment accumulation. Bed B2 could represent a later phase of Pr-2 or a subsequent Priabonian low sea stand (possibly Pr-3).
- Published
- 2014
9. Frontispiz: Zuordnung der Rückgrat- und Seitenketten-Protonen in vollständig protonierten Proteinen durch Festkörper-NMR-Spektroskopie: Mikrokristalle, Sedimente und Amyloidfibrillen
- Author
-
Jan Stanek, Loren B. Andreas, Kristaps Jaudzems, Diane Cala, Daniela Lalli, Andrea Bertarello, Tobias Schubeis, Inara Akopjana, Svetlana Kotelovica, Kaspars Tars, Andrea Pica, Serena Leone, Delia Picone, Zhi-Qiang Xu, Nicholas E. Dixon, Denis Martinez, Mélanie Berbon, Nadia El Mammeri, Abdelmajid Noubhani, Sven Saupe, Birgit Habenstein, Antoine Loquet, and Guido Pintacuda
- Subjects
General Medicine - Published
- 2016
10. High yield of recombinant human apolipoprotein A-I expressed in Pichia pastoris by using mixed-mode chromatography
- Author
-
Krishnan Venkataraman, Abdelmajid Noubhani, Mookambeswaran A. Vijayalakshmi, Vignesh Narasimhan Janakiraman, and Xavier Santarelli
- Subjects
0301 basic medicine ,Tandem mass spectrometry ,Applied Microbiology and Biotechnology ,Pichia ,Pichia pastoris ,law.invention ,03 medical and health sciences ,Tandem Mass Spectrometry ,law ,Humans ,Bioprocess ,Chromatography ,Apolipoprotein A-I ,biology ,Chemistry ,Expanded bed adsorption ,General Medicine ,Chromatography, Ion Exchange ,biology.organism_classification ,Recombinant Proteins ,030104 developmental biology ,Mixed-mode chromatography ,Biochemistry ,Recombinant DNA ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,Target protein - Abstract
A vast majority of the cardioprotective properties exhibited by High-Density Lipoprotein (HDL) is mediated by its major protein component Apolipoprotein A-I (ApoA1). In order to develop a simplified bioprocess for producing recombinant human Apolipoprotein A-I (rhApoA1) in its near-native form, rhApoA1was expressed without the use of an affinity tag in view of its potential therapeutic applications. Expressed in Pichia pastoris at expression levels of 58.2 mg ApoA1 per litre of culture in a reproducible manner, the target protein was purified by mixed-mode chromatography using Capto™ MMC ligand with a purity and recovery of 84% and 68%, respectively. ApoA1 purification was scaled up to Mixed-mode Expanded Bed Adsorption chromatography to establish an 'on-line' process for the efficient capture of rhApoA1 directly from the P. pastoris expression broth. A polishing step using anion exchange chromatography enabled the recovery of ApoA1 up to 96% purity. Purified ApoA1 was identified and verified by RPLC-ESI-Q-TOF mass spectrometry. This two-step process would reduce processing times and therefore costs in comparison to the twelve-step procedure currently used for recovering rhApoA1 from P. pastoris.
- Published
- 2016
11. The selachians’ faunas of the Moroccan phosphate deposits and the KT mass-extinctions
- Author
-
Abdelmajid Noubhani
- Subjects
Extinction event ,High rate ,Extinction ,biology ,Ecology ,Fauna ,Vertebrate ,Phosphate ,Cretaceous ,chemistry.chemical_compound ,Paleontology ,chemistry ,biology.animal ,%22">Fish ,General Agricultural and Biological Sciences ,Geology - Abstract
The Cretaceous and Tertiary phosphate deposits of Morocco which have a vertical thickness covering 25 My (Maastrichtian–Lower Lutetian), are well known for their rich fossil archives. The selachians' fish constitute the most abundant and varied vertebrate faunas. Analysis of the dynamics and patterns of extinctions and faunal renewals identified two significant episodes of high rates of extinction. A mass-extinctions event took place at the end of the Maastrichtian (Cretaceous–Tertiary boundary, KT) when more than 95% of species disappeared. The Lower Ypresian witnessed a second, selective, event which appears to have mainly affected sharks. Geochemical analysis may enlighten the environmental changes.
- Published
- 2010
12. Structure–activity relationship of human liver-expressed antimicrobial peptide 2
- Author
-
Charlotte Cabanne, Milen Milenkov, Bertrand Garbay, Stéphane Chaignepain, Stéphane Claverol, Abdelmajid Noubhani, Xavier Santarelli, Benoit Odaert, Gilles Joucla, Erick J. Dufourc, Caroline Le Sénéchal, Patricia Costaglioli, Agnès Hocquellet, Jean-Marie Schmitter, Wilfrid Dieryck, and Marc Bonneu
- Subjects
Cell Membrane Permeability ,Physiology ,Peptide ,Microbial Sensitivity Tests ,Biology ,Biochemistry ,Protein Structure, Secondary ,law.invention ,Structure-Activity Relationship ,Cellular and Molecular Neuroscience ,Endocrinology ,Protein structure ,law ,Animals ,Humans ,Disulfides ,Protein secondary structure ,Antibacterial agent ,Bacillus megaterium ,chemistry.chemical_classification ,Protein primary structure ,Blood Proteins ,DNA ,Antimicrobial ,biology.organism_classification ,Recombinant Proteins ,Anti-Bacterial Agents ,Protein Structure, Tertiary ,chemistry ,Recombinant DNA ,Oxidation-Reduction ,Antimicrobial Cationic Peptides - Abstract
Liver-expressed antimicrobial peptide 2 (LEAP-2) is a 40-residue cationic peptide originally purified from human blood ultrafiltrate. The native peptide contains two disulfide bonds and is unique regarding its primary structure. Its biological role is not known but a previous study showed that chemically synthesized LEAP-2 exhibited in vitro antimicrobial activities against several Gram-positive bacteria. In order to determine its antimicrobial mode of action, we expressed human recombinant LEAP-2 in Escherichia coli. Circular dichroism spectroscopy and nuclear magnetic resonance analyses showed that the structure of the recombinant peptide was identical to that of the chemically synthesized and oxidized LEAP-2, with two disulfide bonds between Cys residues in relative 1-3 and 2-4 positions. Minimal inhibitory concentration (MIC) of the recombinant human LEAP-2 was determined by a conventional broth dilution assay. It was found to be bactericidal against Bacillus megaterium at a 200microM concentration. Interestingly, the linear LEAP-2 had a greater antimicrobial activity with a MIC value of 12.5microM, which was comparable to that of magainin2. SYTOX Green uptake was used to assess bacterial membrane integrity. Linear LEAP-2 and magainin2 permeabilized B. megaterium membranes with the same efficiency, whereas oxidized LEAP-2 did not induce stain uptake. Binding of the peptides to plasmid DNA was evaluated by gel retardation assays. The DNA-binding efficacy of linear LEAP-2 was three times higher than that of the peptide-containing disulfide bridges. Altogether, these results show that the secondary structure of human LEAP-2 has a profound impact on its antibacterial activity.
- Published
- 2010
13. Variabilité dentaire et crânienne de Numidotherium koholense (Mammalia, Proboscidea) de l’Éocène d’El Kohol, Algérie
- Author
-
Mohammed Mahboubi, Abdelmajid Noubhani, Jean-Jacques Jaeger, Lionel Hautier, and Rodolphe Tabuce
- Subjects
0106 biological sciences ,010506 paleontology ,education.field_of_study ,biology ,Population ,Paleontology ,Biostratigraphy ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Proboscidea ,Theria ,stomatognathic system ,Eutheria ,Space and Planetary Science ,Phanerozoic ,education ,Cenozoic ,Numidotherium ,0105 earth and related environmental sciences - Abstract
The variability of one of the oldest known proboscideans, Numidotherium koholense from the Lower Eocene of El Kohol, Algeria, is here examined. The biometrical study of the jugal teeth shows that the population is homogeneous and corresponds to a single species. The important variations of the skull's shape are interpreted as resulting from sexual dimorphism. The dental variability is analysed and discussed from an evolutionary perspective regarding the adaptative radiation of the proboscidean order at the beginning of the Tertiary.
- Published
- 2008
14. Geoheritage and Geoparks in Africa and the Middle-East: Challenges and Perspectives
- Author
-
Kmar Lattrache, Lala Andrianaivo, Abdulkarim Al-Subbary, Luis Costa, Mamoon Allan, Sophie Siby, Mohammed A. Al-Wosabi, Abderrahmane Bendaoud, Peter Bobrowsky, Marcelle Gauly, Lopo Vasconcelos, Salisu Lawal Halliru, Béatrice Ketchemen, Tea Juliette, Nick Norman, Mohsen Hassine, Gbenga Okunlola, Nasser Ennih, Hasina Nirina Randrianaly, Gabi Schneider, Percy Mabvuto-Ngwira, Fawaz Azki, Abdelmajid Noubhani, Ezzoura Errami, and Monica Omulo
- Subjects
Sustainable development ,Middle East ,Promotion (rank) ,Geodiversity ,Geography ,Geoheritage ,media_common.quotation_subject ,Sustainability ,Geotourism ,Local population ,Environmental planning ,media_common - Abstract
Africa and the Middle East consist of a rich geodiversity, which is regrettably not well known by the public. This is due partly to limited research and studies undertaken in geoheritage and geoconservation in these parts of world, especially those with the intent to explore, inventory and valorize such inherent geodiversity. With the aim to improve this situation, the African Geoparks Network (AGN) was created to increase the awareness of the local population and decision makers regarding the need for sustainable use and management of geoheritage in particular for the benefit of local socio-economic sustainable development targets through the promotion of both geotourism and the creation of unique geoparks.
- Published
- 2015
15. Late Cretaceous and Lower Paleogene Moroccan Phosphates: Geotourism Opportunities
- Author
-
Abdelmajid Noubhani
- Subjects
Sustainable development ,Paleontology ,Sustainability ,Geopark ,Geotourism ,Context (language use) ,Stratigraphy (archaeology) ,Paleogene ,Archaeology ,Cretaceous ,Geology - Abstract
Late Cretaceous and lower Paleogene Moroccan phosphate deposits are one of the best examples of Moroccan geological heritage. These deposits are rich in well-preserved fossil remains including giant marine reptiles, as well as teeth of extinct sharks and rays which have been collected in vast quantities. The Cherifian Office of Phosphates Group (OCP) has recently outlined a strategy to promote sustainable ecological development. In this context, certain sites within the phosphate deposits could be incorporated into a modern Geosite Trail for tourism. Such a trail might start with a visit to the “Museum of Palaeontology” planned in the mining town of Khouribga. This would position visitors within walking distance of sites to be designated within the mining areas, including key representative sections of different geological strata. Besides showcasing the natural beauty of the landscape, these sections present a panoramic view of the lithology and stratigraphy of the phosphate series, displaying both geological features and mining operations. To ensure sustainability, the area would need to be declared a Geopark where geological activities are encouraged but regulated. In addition to the protection and the enhancement of the palaeontological riches, such a declaration would benefit the economy of the local population for whom collecting fossils is a principle economic activity supplementing agriculture and cattle breeding.
- Published
- 2015
16. Aznag (Ouarzazate basin, Morocco), a new African middle Eocene (Lutetian) vertebrate-bearing locality with selachians and mammals
- Author
-
Frédéric Quillévéré, Abdelmajid Noubhani, Henri Cappetta, Rodolphe Tabuce, and Sylvain Adnet
- Subjects
010506 paleontology ,Geology ,14. Life underwater ,15. Life on land ,010502 geochemistry & geophysics ,01 natural sciences ,Humanities ,0105 earth and related environmental sciences - Abstract
Early Paleogene mammals are rare in Africa. They are mainly found in the northwestern part of the continent. Nearshore marine deposits from the Ouarzazate basin, in the southern rim of the Central High Atlas (Morocco), have yielded diverse Thanetian and Ypresian selachian and mammalian faunas [Cappetta et al., 1978, 1987; Gheerbrant et al., 1993]. These faunas from the Paleocene/Eocene transition document the oldest known placental mammals of Africa [Gheerbrant, 1992, 1994, 1995; Gheerbrant et al., 1998; Sigé et al., 1990; Sudre et al., 1993]. New field work in the central part of the basin, near El-Kelaâ M’Gouna, has led to the discovery of a new locality with continental and marine remains, including some non-determinable ostracods and charophytes plus planktonic and benthic foraminifera. The tests of the planktonic foraminifera were globally poorly preserved and highly re-crystallized. However, we found Globigerapsis index (dominant in the sample), Globigerapsis aff. kugleri, Subbotina frontosa, Subbotina inaequispira, Acarinina bullbrooki, Acarinina aff. pentacamerata, Morozovella aragonensis, Morozovella caucasica, Morozovella crater and Pseudohastigerina sp. Following Berggren et al. [1995], the lowest occurrence of the genus Globigerapsis denotes the P10/P11 zonal boundary and the highest occurrence of M. aragonensis denotes the P11/P12 zonal boundary. Both these taxa were represented by several relatively well-preserved specimens. Then, we consider that the sample is correlative to the planktonic foraminiferal Zone P11, for which a biochronal equivalent has been dated between 45.8 and 43.6 Ma. Consequently, the middle Lutetian Aznag locality represents the only known Eocene mammalian site to be adequately dated in Africa. The selachian fauna is relatively rich with nearly 30 species, many of them being probably new. The genera Squatiscyllium, Protoginglymostoma, Ouledia and Garabatis are confidently identified; these taxa were unknown in the Lutetian. We mention the first occurrence in the Eocene of Morocco of « septentrional » taxa Protoginglymostoma, Hemiscyllium and Rhinobatos bruxelliensis and we confirm the presence of genera Eomobula and Rhynchobatus. These data suggest significant North to South faunal exchanges, that contrast with the South to North tendency observed during the early Eocene [Noubhani and Cappetta, 1997]. Nevertheless, the selachian fauna from Aznag is clearly tropical with the occurrence of endemic taxa only known from Morocco (Garabatis, Orectolobiformes nov. gen.) and from the equatorial Tethys-Central Atlantic (Chiloscyllium aff. meraense, Ginglymostoma aff. angolense, Squatiscyllium, « Dasyatis » aff. sudrei, Ouledia, Arechia, Odontorhytis). Only 15 mammalian teeth have been recovered, most of them are very fragmentary and of small size; they document at least 7 species. A probable soricomorph “insectivore” documented by a complete lower molar is very atypical by its tiny size and the entoconid and hypoconulid poorly differentiated from the postcristid. Another “insectivore” is documented by a trigonid of p4, it is reminiscent to the zalambdodont groups. An incisor of rodent shows an enamel microstructure with uniserial Hunter-Schreger bands ; this structure is observed in several Eocene rodents from Eurasia and could be related to the pauciserial to uniserial transition observed in Zegdoumys sebtlai from Chambi (early Eocene, Tunisia) [Martin, 1999]. An upper molar of chiropteran cannot be assigned to one of the known microchiropteran superfamilies; the species from Aznag is characterized by derived traits (e.g. crestiform protocone, hypocone present) and primitive ones such as the presence of a paraconule which evokes the “eochiropteran” grade and the primitive vespertilionoids (paleochiropterygids). A small primate is documented by two fragmentary lower molars and characterized by a well-marked bunodonty and a large paraconid ; this form differs from contemporaneous anthropoids but is very similar to Altiatlasius from the Thanetian of the Adrar Mgorn 1. Two “condylarths” evoke European taxa; a M3 is tentatively assigned to the genus Paschatherium and a DP3or4 is reminiscent to that of Microhyus. This specimen shows also affinities with the ?DP4 of Chambius, a primitive macroscelidid from Chambi. The presence of these mammals strengthen the hypothesis of faunal exchanges between Africa and Europe during the early Eocene. During the middle Eocene, in the south of the Central High Atlas, palynologic data suggested a fairly dry, scarcely vegetated hinterland, and a tropical coastal vegetation (mangrove swamp and salt-marsh) [Fechner, 1988]. The mammals of Aznag, which are characteristic of a closed environment (chiropterans, primate, small ungulates), were certainly confined to a thin belt of coastal vegetation (forest gallery). The fossiliferous level is characterized by a cyclicity of the deposits which could suggest a seasonal variation. Among the selacians, the abundance of the batoids, the absence of both macrofauna and deep-water selacians, and the fragmentary elements indicate hydrodynamical transport in a shallow channel or delta.
- Published
- 2005
17. Production and purification of recombinant human hepcidin-25 with authentic N and C-termini
- Author
-
Caroline Le Sénéchal, Gilles Joucla, Agnès Hocquellet, Vignesh Narasimhan Janakiraman, Patricia Costaglioli, Abdelmajid Noubhani, Wilfrid Dieryck, Charlotte Cabanne, Stéphane Chaignepain, Bertrand Garbay, Xavier Santarelli, Biotechnologie des protéines recombinantes à visée santé, Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 3 LCPO : Polymer Self-Assembly & Life Sciences, and Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)
- Subjects
Hepcidin ,Bioengineering ,Peptide ,Urine ,Applied Microbiology and Biotechnology ,Chromatography, Affinity ,Pichia ,Pichia pastoris ,law.invention ,Recombinant expression ,Hepcidins ,law ,Expanded bed adsorption ,Humans ,chemistry.chemical_classification ,biology ,General Medicine ,biology.organism_classification ,Antimicrobial ,Molecular biology ,Recombinant Proteins ,3. Good health ,[CHIM.POLY]Chemical Sciences/Polymers ,chemistry ,Recombinant DNA ,biology.protein ,P. pastoris ,IMAC ,Antibacterial activity ,Biotechnology - Abstract
International audience; Hepcidin was first identified as an antimicrobial peptide present in human serum and urine. It was later demonstrated that hepcidin is the long-sought hormone that regulates iron homeostasis in mammals. Recombinant human Hepcidin-25 (Hepc25) was expressed in Pichia pastoris using a modified version of the pPICZ alpha A vector. Hepc25 was then purified by a simple two-step chromatographic process to obtain 1.9 mg of soluble recombinant human Hepc25 per liter of culture at 96% purity. The sequence of Hepc25 and the presence of four disulfide bridges were confirmed by mass spectrometry analyses, and the recombinant Hepc25 exhibited antibacterial activity. This protocol of production and purification is the first step toward the production of human Hepc25 at a greater scale. (C) 2015 Elsevier B.V. All rights reserved.
- Published
- 2014
18. The Trehalose Pathway Regulates Mitochondrial Respiratory Chain Content through Hexokinase 2 and cAMP in Saccharomyces cerevisiae*
- Author
-
Beatriz M. Bonini, Abdelmajid Noubhani, Michel Rigoulet, Johan M. Thevelein, Odile Bunoust, Anne Devin, and Grellety, Marie-Lise
- Subjects
Saccharomyces cerevisiae ,Oxidative phosphorylation ,Biology ,Mitochondrion ,Biochemistry ,Electron Transport ,chemistry.chemical_compound ,Hexokinase ,Cyclic AMP ,Glycolysis ,Phosphorylation ,Molecular Biology ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,ComputingMilieux_MISCELLANEOUS ,ATP synthase ,Temperature ,Trehalose ,Cell Biology ,biology.organism_classification ,Cell biology ,Mitochondria ,Metabolism and Bioenergetics ,Kinetics ,Mitochondrial respiratory chain ,chemistry ,Glucosyltransferases ,Mutation ,biology.protein ,Cytochromes - Abstract
In yeast, trehalose is synthesized by a multimeric enzymatic complex: TPS1 encodes trehalose 6-phosphate synthase, which belongs to a complex that is composed of at least three other subunits, including trehalose 6-phosphate phosphatase Tps2 and the redundant regulatory subunits Tps3 and Tsl1. The product of the TPS1 gene plays an essential role in the control of the glycolytic pathway by restricting the influx of glucose into glycolysis. In this paper, we investigated whether the trehalose synthesis pathway could be involved in the control of the other energy-generating pathway: oxidative phosphorylation. We show that the different mutants of the trehalose synthesis pathway (tps1Delta, tps2Delta, and tps1,2Delta) exhibit modulation in the amount of respiratory chains, in terms of cytochrome content and maximal respiratory activity. Furthermore, these variations in mitochondrial enzymatic content are positively linked to the intracellular concentration in cAMP that is modulated by Tps1p through hexokinase2. This is the first time that a pathway involved in sugar storage, i.e. trehalose, is shown to regulate the mitochondrial enzymatic content.
- Published
- 2009
19. Biochemical changes induced by accelerated aging in sunflower seeds. II. RNA populations and protein synthesis
- Author
-
Xavier Gidrol, Alain Pradet, Abdelmajid Noubhani, ProdInra, Migration, Station de physiologie végétale, and Institut National de la Recherche Agronomique (INRA)
- Subjects
Senescence ,Physiology ,food and beverages ,Cell Biology ,Plant Science ,General Medicine ,Biology ,BIOLOGIE MOLECULAIRE ,Accelerated aging ,Sunflower ,Biochemistry ,Ageing ,Gene expression ,Helianthus annuus ,Protein biosynthesis ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,VIELLISSEMENT ,Imbibition ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology - Abstract
Sunflower seeds (Helianthus annuus L. cv Rodeo) slowly lose their viability when subjected to accelerated aging (42°C and 100% relative humidity). After 12 h imbibition, in vivo protein labelling profiles show dramatic changes as a function of accelerated aging. Total and poly(A+)-RNA content of embryonic axes were similar regardless of the time spent in hot and wet conditions. However, the ability of mRNA to be translated in cell-free system was largely reduced in acceleratedly aged seeds. In vitro protein synthesis revealed the observed changes to be largely quantitative rather than qualitative, and to result mainly from post-transcriptional regulation. However, the induction of two proteins (15 and 43 kDa) subsequent to accelerated aging resulted from an increase in their corresponding mRNA, The accelerated aging treatment of sunflower seeds primarily induces quantitative changes in the translation of pre-existing mRNA, rather than the production of new mRNA species.
- Published
- 1990
20. Large-scale production, bacterial localization assessment and immobilized metal affinity chromatography purification of a human single-chain Fv antibody against alphaIIb-beta3 integrin
- Author
-
Marie-Josée Jacobin-Valat, Abdelmajid Noubhani, Agnès Hocquellet, Danièle Daret, Gisèle Clofent-Sanchez, Remy Robert, and Xavier Santarelli
- Subjects
Protein Folding ,Recombinant Fusion Proteins ,Immunoglobulin Variable Region ,Platelet Glycoprotein GPIIb-IIIa Complex ,medicine.disease_cause ,Biochemistry ,Inclusion bodies ,Flow cytometry ,Affinity chromatography ,Structural Biology ,In vivo ,medicine ,Escherichia coli ,Animals ,Humans ,Molecular Biology ,Inclusion Bodies ,Chromatography ,biology ,medicine.diagnostic_test ,Chemistry ,Antibodies, Monoclonal ,Thrombosis ,General Medicine ,respiratory system ,Atherosclerosis ,Disease Models, Animal ,Cytoplasm ,biology.protein ,Rabbits ,Antibody ,Chromatography, Liquid - Abstract
Our objective was to investigate the Escherichia coli localization (such as supernatant, cytoplasm and inclusion bodies) of an anti-alphaIIb-beta3 (alphaIIbbeta3) scFv fragment referred to as scFv[EBB3] produced in batch fermentation. Immobilized metal affinity chromatography (IMAC) purification was performed on supernatant using expanded bed absorbed technology (EBA) and on sonicated cells in native conditions over an immobilized copper-ion affinity column. Inclusion bodies were solubilized before IMAC purification and the refolding procedure was performed on the column. The majority of scFv[EBB3] were present as inclusion bodies (55%), whereas 36% were found in the cytoplasm and only 9% secreted in the supernatant. The scFv activity was assessed by enzyme-linked immunosorbent assay (ELISA), flow cytometry and immunohistochemistry analyses performed on a thrombus induced in vivo on an atherosclerotic rabbit model.
- Published
- 2005
21. Ypr140wp, ‘the yeast tafazzin’, displays a mitochondrial lysophosphatidylcholine (lyso-PC) acyltransferase activity related to triacylglycerol and mitochondrial lipid synthesis
- Author
-
Stéphen Manon, Odile Bunoust, Eric Testet, Denis Coulon, René Lessire, Jeanny Laroche-Traineau, Nadine Camougrand, Abdelmajid Noubhani, and Jean-Jacques Bessoule
- Subjects
Saccharomyces cerevisiae Proteins ,Mutant ,Molecular Sequence Data ,Tafazzin ,Gene Expression ,Saccharomyces cerevisiae ,Mitochondrion ,Biochemistry ,chemistry.chemical_compound ,Cardiolipin ,Escherichia coli ,Amino Acid Sequence ,Molecular Biology ,Triglycerides ,biology ,Sequence Homology, Amino Acid ,Cell Membrane ,1-Acylglycerophosphocholine O-Acyltransferase ,Lipid metabolism ,Cell Biology ,Lipids ,Yeast ,Mitochondria ,Lysophosphatidylcholine ,chemistry ,Acyltransferase ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Acyltransferases ,Research Article - Abstract
When the yeast protein Ypr140w was expressed in Escherichia coli, a lyso-PC [lysophosphatidylcholine (1-acylglycerophosphorylcholine)] acyltransferase activity was found associated with the membranes of the bacteria. To our knowledge, this is the first identification of a protein capable of catalysing the acylation of lyso-PC molecules to form PC. Fluorescence microscopy analysis of living yeasts revealed that the fusion protein Ypr140w–green fluorescent protein is targeted to the mitochondria. Moreover, in contrast with wild-type cells, in the absence of acyl-CoA, the yeast mutant deleted for the YPR140w gene has no lyso-PC acyltransferase activity associated with the mitochondrial fraction. When yeast cells were grown in the presence of lactate, the mutant synthesized 2-fold more triacylglycerols when compared with the wild-type. Moreover, its mitochondrial membranes contained a lesser amount of PC and cardiolipin, and the fatty acid composition of these latter was greatly changed. These modifications were accompanied by a 2-fold increase in the respiration rates (states 3 and 4) of the mitochondria. The relationship between the deletion of the YPR140w gene and the lipid composition of the ypr140wΔ cells is discussed.
- Published
- 2005
22. Aznag (bassin d'Ouarzazate, Maroc), nouvelle localité à sélaciens et mammifères de l'Eocène moyen (Lutétien) d'Afrique
- Author
-
Rodolphe Tabuce, Sylvain Adnet, Henri Cappetta, Abdelmajid Noubhani, Frédéric Quillevéré, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), département de géologie, Université Chouaib Doukkali (UCD), PaleoEnvironnements et PaleobioSphere (PEPS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Sélaciens ,Afrique ,Foraminifères ,Bassin d'Ouarzazate ,Lutétien ,[SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/Paleontology ,ComputingMilieux_MISCELLANEOUS ,Mammifères - Abstract
International audience
- Published
- 2005
23. Improvement in production and purification bioprocesses of bacterially expressed anti-alphaIIbbeta3 human single-chain FV antibodies
- Author
-
Xavier Santarelli, Remy Robert, Marie-Josée Jacobin, Abdelmajid Noubhani, and Gisèle Clofent-Sanchez
- Subjects
Blood Platelets ,Male ,Clinical Biochemistry ,Immunoglobulin Variable Region ,lac operon ,Enzyme-Linked Immunosorbent Assay ,Platelet Glycoprotein GPIIb-IIIa Complex ,Transfection ,Biochemistry ,Chromatography, Affinity ,Analytical Chemistry ,Flow cytometry ,Affinity chromatography ,In vivo ,medicine ,Escherichia coli ,Animals ,Immunoglobulin Fragments ,Gel electrophoresis ,Chromatography ,medicine.diagnostic_test ,biology ,Chemistry ,Thrombosis ,Cell Biology ,General Medicine ,Flow Cytometry ,Immunohistochemistry ,Fermentation ,biology.protein ,Rabbits ,Antibody - Abstract
Production of anti-alphaIIbbeta3 (anti-alphaIIbbeta3)-binding single-chain FV (scFv) fragments obtained from combinatorial libraries of IgG human antibodies is of broad interest for imaging and treatment of acute coronary syndromes. The objective of our work was to design an optimized production of one selected anti-alphaIIbbeta3-binding scFv fragment for subsequent in vivo animal studies. Fed-batch fermentation was initiated with 2TY media supplemented with 0.1 M glucose. This growing batch culture was used as a starting point for further fed-batch induction, in which a media without glucose containing 1 mM IPTG and 0.4 M saccharose was continuously added. Subsequent purification was performed on the whole cell extract in native conditions over an immobilized copper-ion affinity column. The improved conditions allowed the recovery of 5 mg of highly purified scFv fragments as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The bioactivity of the scFv fragments was further monitored by ELISA, cytometric and immunohistochemical methods.
- Published
- 2004
24. S5.5 The trehalose pathway regulates mitochondrial respiratory chain content through hexokinase2 and AMPc IN Saccharomyces cerevisiae
- Author
-
Abdelmajid Noubhani, Johan M. Thevelein, Beatriz M. Bonini, Odile Bunoust, Anne Devin, and Michel Rigoulet
- Subjects
chemistry.chemical_compound ,Mitochondrial respiratory chain ,Biochemistry ,biology ,Chemistry ,Saccharomyces cerevisiae ,Biophysics ,Cell Biology ,biology.organism_classification ,Trehalose - Published
- 2008
25. Les Vertébrés des phosphates crétacés-paléogènes (72,1-47,8 Ma) du Maroc
- Author
-
Nathalie Bardet, Emmanuel Gheerbrant, Abdelmajid Noubhani, Henri Cappetta, Stéphane Jouve, Estelle Bourdon, Xabier Suberbiola, Nour-Eddine Jalil, Peggy Vincent, Alexandra Houssaye, Floréal Solé, Khadija Elhoussaini Darif, Sylvain Adnet, Jean-Claude Rage, France de Lapparent de Broin, Jean Sudre, Baâdi Bouya, Mbarek Amaghzaz, Saïd Meslouh, Centre de recherche sur la Paléobiodiversité et les Paléoenvironnements (CR2P), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Section of Biosystematics, Natural History Museum of Denmark, Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH)-Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH), Mécanismes Adaptatifs et Evolution (MECADEV), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Royal Belgian Institute of Natural Sciences (RBINS), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU)-Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)
- Subjects
Maroc ,Sélaciens ,Oiseaux ,Poissons osseux ,Crises K/Pg et P/E ,Maastrichtien-Lutétien ,Reptiles ,Point chaud de paléobiodiversité ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,Domaine sud-téthysien ,Phosphates ,Mammifères - Abstract
International audience; Les phosphates du Maroc s’étendent sur une période d’environ 24 millions d’années(Ma), de la fin du Crétacé (Maastrichtien) à la base de l’Eocène moyen (Lutétien), c’est-à-direla plus longue durée de tous les dépôts phosphatés des « Provinces Phosphogéniques »méditerranéenne et atlantique. Ces sédiments marins ont enregistré l’évolution de la vie dansles mers épicontinentales en marge du Craton ouest-africain et de son arrière pays continental ;ils se caractérisent par leur remarquable richesse en restes de vertébrés fossiles, qui illustre un« point-chaud » de paléobiodiversité quasi-unique au monde, au tournant Crétacé-Tertiaire, enliaison directe avec les apports de nutriments organiques par les courants d’upwellings profondssur la marge océanique Ouest africaine.Ces dépôts se singularisent par une combinaison singulière de facteurs qui en soulignent lavaleur : 1) les découvertes paléontologiques des bassins des Oulad Abdoun et des Ganntourintéressent l’ensemble de la série phosphatée et des grands groupes de vertébrés ; 2) les fossiles sontexceptionnels tant par leur abondance et leur diversité, que par la qualité de leur conservation ; 3)ils documentent une période-clé dans l’histoire évolutive des vertébrés qui peut être suivie in situsur 24 Ma et qui enregistre deux crises biologiques majeures (limites Crétacé/Tertiaire (K/Pg)et Paléocène/Eocène (P/E)) ; 4) ils se situent dans des provinces paléobiogéographiques encoremal connues (marges sud-téthysienne et atlantique et province arabo-africaine) ; 5) l’exploitationscientifique de ces gisements est possible sur le long terme ; 6) les fossiles récoltés ont unegrande valeur à la fois scientifique (plusieurs recherches en cours), mais également patrimoniale(collection de référence mondiale), et muséologique (création d’un musée).Depuis les travaux pionniers du paléontologue français Camille Arambourg publiés entre1935 et 1952, ce patrimoine paléontologique exceptionnel est néanmoins resté sous-exploitéscientifiquement à l’exception des sélaciens, abondamment étudiés. La découverte inattendueen 1996 de restes de mammifères terrestres dans ces phosphates marins a ravivé l’intérêt despaléontologues et a entraîné la signature en 1997 d’une Convention de recherche franco-marocaineentre le Centre National de la Recherche Scientifique (CNRS), le Muséum National d’HistoireNaturelle (MNHN), le Groupe Office Chérifien des Phosphates (OCP) et le Ministère de l’Energie,des Mines, de l’Eau et de l’Environnement (MEMEE). En 2005, cette Convention a été élargie auxUniversités Cadi Ayyad de Marrakech (UCAM) et Chouaîb Doukkali d’El Jadida (UCDJ). Notretravail multidisciplinaire mené dans le cadre de cette Convention dite « Phosphapal » a permisnon seulement d’améliorer nos connaissances sur la systématique des vertébrés fossiles desphosphates du Maroc, leurs paléoenvironnements et répartitions paléobiogéographiques, maiségalement de constituer une importante collection paléontologique de référence qui alimente leprojet de création par l’OCP d’un musée dans la ville de Khouribga.Les restes fossiles des phosphates du Maroc illustrent tous les groupes de vertébrés, à l’exceptiondes amphibiens : poissons cartilagineux (sélaciens) et osseux (actinoptérygiens), reptiles (oiseauxinclus) et mammifères, soit en l’état actuel des connaissances 332 espèces, 192 genres et 86familles. La plupart des taxons sont marins mais quelques très rares restes d’animaux terrestresont également été découverts. Plusieurs de ces groupes, tels que les dinosaures non aviens, lesoiseaux, les ptérosaures et les mammifères étaient totalement inconnus du temps des travauxd’Arambourg.3Les sélaciens sont de loin les fossiles les plus abondants et diversifiés avec 250 espèces de requinset de raies. En plus de leur intérêt purement paléontologique (taxonomie), ils représententun outil biostratigraphique de référence pour la datation et la corrélation des phosphates del’ensemble de la marge sud-téthysienne. Les poissons actinoptérygiens sont surtout représentéspar des téléostéens et sont abondants dans tous les niveaux. Ils demeurent largement inédits endehors de quelques études ponctuelles. Les reptiles (y compris les oiseaux) sont également trèsabondants et diversifiés (au moins 55 espèces) et souvent représentés par des spécimens completset spectaculaires. Mis à part quelques taxons terrestres (dinosaures non aviens et ptérosaures),ils incluent des formes majoritairement marines appartenant aux chéloniens, squamates,plésiosauriens, crocodyliformes et oiseaux. Cette paléoherpétofaune des phosphates du Marocreprésente une référence mondiale pour les faunes de reptiles marins du Crétacé supérieur -Paléogène inférieur de la marge Sud-téthysienne. Les mammifères continentaux, groupe le plusinattendu dans ces dépôts marins, sont représentés par au moins dix espèces appartenant auxordres des hyaenodontidés, proboscidiens, hyracoïdes et condylarthres. Ils figurent parmi lesplus anciens placentaires d’Afrique et les plus anciens fossiles connus d’ongulés modernes aumonde.
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.