1. Protective effect of chondroitin sulfate nano-selenium on chondrocyte of patients with Kashin-Beck disease.
- Author
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Qiao, Lichun, Amhare, Abebe F, Deng, Huan, Lv, Yizhen, Zhao, Yan, Liu, Jiaxin, Lei, Jian, Wang, Liyun, Chilufya, Mumba M, and Han, Jing
- Subjects
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CHONDROITIN sulfates , *CHONDROITIN sulfate proteoglycan , *TRANSMISSION electron microscopy , *POLYMERASE chain reaction , *SULFOTRANSFERASES - Abstract
Objective: To investigate the protective effect of chondroitin sulfate nano-selenium (SeCS) on chondrocyte of Kashin-Beck disease (KBD). Methods: Chondrocyte samples were isolated from the cartilage of three male KBD patients (54–57 years old). The chondrocytes were respectively divided into four groups: (a) control group, (b) SeCS supplement group (100 ng/mL SeCS), (c) T-2 + SeCS supplement group (20 ng/mL T-2 + 100 ng/mL SeCS), and (d) T-2 group (20 ng/mL T-2). Live/dead staining and transmission electron microscopy (TEM) were used to observe cell viability and ultrastructural changes in chondrocytes respectively. Expressions of Caspase-9, cytochrome C (Cyt-C), and chondroitin sulfate (CS) structure-modifying sulfotransferases including carbohydrate sulfotransferase 3, 15 (CHST-3, CHST-15), and uronyl 2-O-sulfotransferase (UST) were examined by quantitative real-time polymerase chain reaction. Results: After one- or three-days intervention, the number of living chondrocytes in the SeCS supplement group was higher than that in the control group, while it is opposite in the T-2 + SeCS supplement group and T-2 group. The cellular villi number in the surface increased in the SeCS supplement group compared with the control group. Mitochondrial morphology density was improved in the T-2 + SeCS supplement group compared with the T-2 group. Expressions of CHST-3, CHST-15, UST, Caspase-9, and Cyt-C on the mRNA level significantly increased in the T-2 + SeCS supplement group and T-2 group compared with the control group. Conclusions: SeCS supplement increased the number of living chondrocytes, improved the ultrastructure, and altered the expressions of CS structure-modifying sulfotransferases, Caspase-9, and Cyt-C. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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