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Numerous biological and chemical methods have been proposed over the years for estimating the nitrogen (N) mineralization capacity of soils; however, none of them has found general use in soil fertility testing. The efficacy of a recently proposed alkaline hydrolysis method for assessing N availability in soils compared with the standard long-term incubation technique for determining potentially available N was evaluated. The nitrogen mineralization of 12 surface soils incubated under aerobic conditions at 25 °C for 26 weeks was determined. Field-moist soils were direct-steam distilled with 1 M KOH or 1 M NaOH; the NH3 released was trapped in boric acid, and its concentration was determined successively every 5 min for 40 min. The cumulative N mineralized or hydrolyzed was fitted to the first-order exponential equation to determine the potentially mineralizable N (No) and an analogous “potentially hydrolyzable N (Nmax)” for the soils. The flush of CO2 (fCO2) following the rewetting and incubation of air-dried soils under aerobic conditions for 3 days was also determined. The results showed that the Nmax values differed considerably among the soils, indicating differences in the chemical nature and reactivity of the organic N content of the soils, and were significantly correlated with No and fCO2 values. The estimated Nmax and No values ranged from 105 to 371 mg N kg−1 and 121 to 292 mg kg−1, respectively. Based on the simple and inexpensive nature of the alkaline hydrolysis procedure, the reduction in the incubation time required to obtain No (months to minutes), and the strong association between Nmax and No, we concluded that Nmax is a good predictor of the biologically discrete and quantifiable labile pool of mineralizable soil organic N (ON), and the use of the alkaline hydrolyzable ON as a predictor of No merits consideration for routine use in soil testing laboratories for estimating the N-supplying capacity of soils.