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The aim of this work was to reduce the postweaning stress of growing rabbits by using fasting regimen and probiotic supplementation. Twenty‐four weaned males new Zealand rabbits (5 weeks of age) were randomly divided into four equal groups including (1) rabbits fed ad labium diet served as control, (2) rabbits fasted for 12 h (two days/week) for 8 weeks, (3) rabbits fed ad labium diet with 2.0 cm3 probiotic/litter for 8 weeks, (4) rabbits fasted for 12 h (2 days/week) and take 2.0 cm3 probiotic/litter for 8 weeks. The probiotic inclusion corresponded to Bacillus subtilis and Bacillus licheniformis were supplemented to water in the probiotic groups. Results showed that food consumption was significantly higher (p < 0.01) in growing rabbits of ad libitum group (control group) compared to other in restricted rabbits, but body weight (BW) of growing rabbit in probiotic group at 6 and 12 week of age increased by12 and 19%, respectively, than other in control group and also improved daily weight gain (DWG) during all experimental periods. Compared with control, significant reductions (p <.01) in Plasma cholesterol, triglycerides, HDL, LDL, ALT, AST and urea were found in oral probiotic groups with or without fasting programme. Oral administration of probiotic without fasting regimen significantly improved all carcass characteristics compared to other groups. Total bacteria, fungi, coliforms and cellulolytic bacteria counts were significantly improved in the fasting group with or without adding probiotics compared to control group. Conclusively, using probiotics with or without fasting regimen for 8 weeks postweaning improved growth performance during fattening and enhancement cecum activity of growing rabbits. [ABSTRACT FROM AUTHOR]