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11. Contributors

Author

Abraham, Adane, primary, Adhikary, Nayan Kishor, additional, Akhter, Md Shamim, additional, Ali, Akhtar, additional, Ali, Md Emran, additional, Alicai, Titus, additional, Anuradha, C., additional, Bakhtawar, Faheema, additional, Balasubramanian, V., additional, Baranwal, Virendra Kumar, additional, Basavaraj, Y.B., additional, Bhor, Sachin Ashok, additional, Brugo Carivali, María F., additional, Chandel, Vanita, additional, Chandrasekar, A., additional, Gaur, Rajarshi Kumar, additional, Giolitti, Fabián, additional, Hajeri, Subhas, additional, Heo, Kyeong-Jae, additional, Iftikhar, Yasir, additional, Jain, Rakesh Kumar, additional, Jayasinghe, Wikum H., additional, Jeong, Rira, additional, Ji, Chang Yoon, additional, Julian Bujarski, Józef, additional, Karthikeyan, G., additional, Kaya, Takahide, additional, Khurana, S.M.P., additional, Kidanemariam, Dawit, additional, Kim, Minlee, additional, Kobayashi, Kappei, additional, Konakalla, Naga Charan, additional, Kozieł, Edmund, additional, Kumar, Ashwini, additional, Kumar, Jitendra, additional, Latha, T.K.S., additional, Luciani, Cecilia E., additional, Mahalakshmi, M., additional, Masarapu, Hema, additional, Mederos, Dariel Cabrera, additional, Mishra, Vachaspati, additional, Nagendran, K., additional, Nautiyal, Aparna, additional, Nishiguchi, Masamichi, additional, Otulak Kozieł, Katarzyna, additional, Parameshwari, B., additional, Patil, Basavaprabhu L., additional, Patra, Biswajit, additional, Perotto, María C., additional, Petrov, Nikolay Manchev, additional, Popli, Sonam, additional, Portal, Orelvis, additional, Priyanga, T., additional, Radhakrishnan, Thankappan, additional, Rai, Richa, additional, Rajamanickam, S., additional, Ramlal, Ayyagari, additional, Rani, Anita, additional, Rishi, Narayan, additional, Rout, Bichhinna Maitri, additional, Sajid, Ashara, additional, Sanan-Mishra, Neeti, additional, Sarma, Ritasree, additional, Selvarajan, Ramasamy, additional, Sharma, Pooja, additional, Sharma, Susheel Kumar, additional, Singh, Manoj Kumar, additional, Stoyanova, Mariya Ivanova, additional, Suman, Rashmi, additional, Tripathi, Savarni, additional, Trucco, Verónica M., additional, Ullah, Muhammad Irfan, additional, Voloudakis, Andreas E., additional, Wagaba, Henry, additional, Wagh, Sopan Ganpatrao, additional, and Yokomi, Raymond, additional

Published
2023
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20. High‐throughput sequencing‐based detection and characterization of sugarcane viruses in Ethiopia.

Author

Abide, Mereme, Kidanemariam, Dawit, Kebede, Misrak, Knierim, Dennis, Margaria, Paolo, Menzel, Wulf, and Abraham, Adane

Subjects
AMINO acid sequence, WHOLE genome sequencing, VIRUS diversity, VIRAL proteins, SUGARCANE, MOSAIC viruses
Abstract

A virus discovery investigation using high‐throughput sequencing in sugarcane samples from Ethiopia revealed the presence of sugarcane mild mosaic virus (SCMMV, genus Ampelovirus) and sugarcane umbra‐like virus (SULV, genus Umbravirus). The genome sequences of two isolates of SCMMV and one SULV were determined. The sequences of the two SCMMV isolates were 13,005 nucleotides in length and showed ~73.5% nucleotide identity along the genome and ~90.2, 96.8, and 90.4% amino acid sequence identity among each other in the RdRp, CP, and HSP70h, respectively. Isolate SCMMV ET2 showed a close relationship to group A isolates from Colombia, the USA, and the Philippines, with amino acid sequence identity of the predicted virus proteins in the range of 94–98.9%. Conversely, SCMMV ET1 shared a closer relationship with group B isolates from Colombia, Ivory Coast, and Argentina, exhibiting a 93–99% amino acid sequence identity. The complete genome sequence of SULV, comprising 3041 nucleotides, exhibited the highest identity with its counterpart from South Africa (MN868593). These findings contribute to expanding our understanding of the viral diversity within the sugarcane crop in Ethiopia. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Survey of farmers' knowledge of yam pests and diseases and management practices in southern Ethiopia.

Author

Gogile, Ashebir, Kebede, Misrak, Wada, Eyasu, Kidanemariam, Dawit, and Abraham, Adane

Subjects
PEST control, INSECT pathogens, AGRICULTURAL productivity, CROP rotation, DISEASE management
Abstract

Yam is a widely consumed food in the tropics and subtropics. Determination of pests (pathogens and insects) and diseases (developed pest invasions and symptoms) and the appropriate management practices are critical in existing crop production systems. This study aimed to document farmers' knowledge about yam pests and diseases and their management practices in southern Ethiopia. Data were collected from 342 systematically selected farmers and 96 yam fields in 5 major yam-growing zones (Dawuro, Gofa, Gamo, Kembata-Tembaro, and Wolaita) in southern Ethiopia using a semi-structured interview guide. The results showed that 54.1% of the farmers saved their planting materials for the next growing season, while 39.2% of the farmers purchased the planting materials from the local market every year. Most farmers (63.5%) reported that the cultivation of yam has been declining annually due to pests and diseases. White yam (Dioscorea rotundata) (Poir, Dioscoreales, Dioscoreaceae) and water yam (Dioscorea alata) are severely damaged by pests and diseases, as perceived by 33.9% and 24.9% of farmers, respectively. Farmers use healthy-looking pest- and disease-free tubers (39.5%), remove infected plants (24.0%), practice crop rotations (17.3%), and use animal manure (7.0%) to manage yam pests and diseases. Farmers' knowledge can be a starting point for seeking solutions to yam pests and disease risks. This study could play an important role in improving yam cultivation by identifying strategies to improve the recent decline in yam production to meet future food needs for a rapidly growing population. [ABSTRACT FROM AUTHOR]

Published
2024
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36. Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virus isolates

Author

Kidanemariam, Dawit B., Sukal, Amit C., Abraham, Adane D., Njuguna, Joyce N., Stomeo, Francesca, Dale, James L., James, Anthony P., Harding, Robert M., Kidanemariam, Dawit B., Sukal, Amit C., Abraham, Adane D., Njuguna, Joyce N., Stomeo, Francesca, Dale, James L., James, Anthony P., and Harding, Robert M.

Abstract

Taro (Colocasia esculenta) and tannia (Xanthosoma sp.) plants growing in 25 districts across Ethiopia, Kenya, Tanzania and Uganda were surveyed for four RNA viruses. Leaf samples from 392 plants were tested for cucumber mosaic virus (CMV), dasheen mosaic virus (DsMV), taro vein chlorosis virus (TaVCV) and Colocasia bobone disease-associated virus (CBDaV) by RT-PCR. No samples tested positive for TaVCV or CBDaV, while CMV was only detected in three tannia samples with mosaic symptoms from Uganda. DsMV was detected in 40 samples, including 36 out of 171 from Ethiopia, one out of 94 from Uganda and three out of 41 from Tanzania, while none of the 86 samples from Kenya tested positive for any of the four viruses. The complete genomes of nine DsMV isolates from East Africa were cloned and sequenced. Phylogenetic analyses based on the amino acid sequence of the DsMV CP-coding region revealed two distinct clades. Isolates from Ethiopia were distributed in both clades, while samples from Uganda and Tanzania belong to different clades. Seven possible recombination events were identified from the analysis carried out on the available 15 full-length DsMV isolates. Nucleotide substitution ratio analysis revealed that all the DsMV genes are under strong negative selection pressure.

Published
2022

40. Serological, biological and molecular characterization of viruses causing mosaic diseases on cabbage (Brassicasp L.)in Central Ethiopia

Author

Kebede, Muluken, Guadie, Demsachew, Kidanemariam, Dawit, and Abraham, Adane

Abstract

The productivity of cabbage (Brassica oleraceavar. capitata)in Ethiopia has been generally low due to several biotic and abiotic constraints among which are several viral diseases. There is a recent report indicating that this economically important vegetable is seriously affected in Ethiopia by cauliflower mosaic virus (CaMV) and turnip mosaic virus (TuMV). However, little information exists on the incidence and distribution of these viruses as the previous report is based on samples only from Addis Ababa. In this study, a total of 370 leaf samples were collected from 75 cabbage growing fields in Central Ethiopia in two rounds of survey. Two cabbage varieties locally known as “Habesha gomen”and “Tikur gomen”with virus-like symptoms were collected and tested with Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) using polyclonal antibodies specific to CaMV and TuMV. Results from serological diagnosis were confirmed with PCR and Sanger sequencing. The results indicated a high incidence and wide distribution of both viruses in Central Ethiopia with an average of 29.5% infection for CaMV and 40% for TuMV. Biological inoculation tests for CaMV or TuMV or both on healthy cabbage seedlings gave similar symptoms as those observed in the field. Symptom severity was higher with co-infection of CaMV and TuMV followed by TuMV single infection. BLAST analysis showed that TuMV and CaMV isolates from Ethiopia have nucleotide identity of 95–98% and 93–98%, respectively to previously reported isolates. Phylogenetic analysis revealed that CaMV isolates from Ethiopia are closely related to isolates from USA and Italy within Group II clade whereas TuMV isolates have close similarities with isolates from World B clade including isolates from Kenya, UK, Japan and the Netherlands. The identification of the causative agents of the mosaic disease observed on cabbage in Central Ethiopia may lay the foundation for future management studies.

Published
2023
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42. Contributors

Author

Abadin, Zain Ul, Abbas, Muhammad Taqqi, Abd El-Aziz, M.H., Abhishek, G.J., Abraham, Adane, Adeniyi, D.O., Ahmad, Sadia, Ahmed, Mukhtar, Ahsan, Aymen, Akinyemi, Sunday O.S., Al-Tawaha, Abdel Razzaq, Alegbejo, Matthew Dada, Alemandri, Vanina, Ali, Akhtar, Aliyu, Taiye H., Amato, Marcos, Amezquita, N.F., Angira, Aniket, Arogundade, Olawale, Arshad, Hibba, Asif, Mahrukh, Aslam, Muhammad Naveed, Asogwa, E.U., Avila, L.A., Awasthi, L.P., Back, Emma J., Bano Rizvi, Syeda Shahar, Banwo, Olalekan Oyeleke, Barbosa, Tarsiane M.C., Barros, S.C.I.M., Barrón-Contreras, J.L., Bañuelos-Hernández, B., Belen, Glenrose B., Beltran, J.H., Berkani, Salima, Bernal, Julio S., Bhardwaj, Pooja, Bhat, Alangar Ishwara, Boonham, N., Bruckner, Fernanda P., Budzanivska, I., Bukhari, Syeda Fatima, Cabrera Mederos, Dariel, Carvalho, C.M., Chalam, V. Celia, Chandra, Prem, Changadeya, Wisdom, Changaya, Albert, Chapara, V., Chapara, R., Chatterjee, Arpita, Choudhary, Nandlal, Cherian K., Anita, Chinnadurai, Chinnaraja, Chirumamilla, A., Chofong, G.N., Clover, G.R.G., Creamer, Rebecca, Cruz-Hernández, A., Cruz, Maylín, Cuevas, A., Dale, J., Das, I.K., da Silva, Marcel Fernando, Davino, Salvatore, Deepika, D.D., Delmiglio, C., Dian, V.S., Dongo, L.N., Echeverri, J., Eiras, Marcelo, Ellouze, Walid, Elçi, Eminur, Emmanuel, C.J., Enespa, Ertunç, Filiz, Faggioli, Francesco, Fall, Mamadou L., Fariña, Arnaldo Esquivel, Farooq, Tahir, Figueira, A.R., Fox, A., Gaafar, Yahya Z.A., Gates, Matthew Russell, Gazel, Mona, Ghosh, Subrata Kumar, Giolitti, Fabián, Gnanaprakash, S., Gonçalves, Marcos Cesar, Goodin, M.M., Groth-Helms, Deborah, Gupta, Taruna, Haider, Muhammad Saleem, Hallan, Vipin, Harding, R., Harish, Atheena, Haroon, Rajia, Hashmi, Muhammad Muneeb, Higuera, O.L., Ighani, Hakimeh, James, A., Jasrotia, Poonam, Javaran, Vahid J., Jevremović, Darko, Jeyaseelan, T.C., Jones, Tara-Kay L., Kalaiponmani, K., Kapoor, Promil, Karanfil, Ali, Karanja, Laura Shali, Karavina, Charles, Karthikeyan, Gandhi, Kashina, Boniface David, Kashyap, Prem Lal, Katis, N.I., Kaul, Noyonika, Kaur, Baljeet, Kaushal, Lovelin, Kawas, Houda, Keremane, Manjunath L., Khaliq, Robina, Khan, Asifa, Khanna, Megha, Khanum, Samia, Kidanemariam, D., Kil, Eui-Joon, Kim, Chang-Seok, Kingsley-Umana, E.B., Kitajima, Elliot Watanabe, Krishnan, Kathiravan, Krishna Reddy, M., Kumar, R., Kumar, Sudheer, Kumar, Surender, Kumari, Safaa G., Kumari, Pooja, Kumari, Reenu, Kyriakopoulou, P.E., Lau, Douglas, Leastro, Mikhail Oliveira, Lee, Richard F., Lee, Sukchan, Leiva, D.C., Leiva Mora, Michel, Londoño-Avendaño, M.A., Londoño, A., Lorenzo, Jaira T., Maciel, L.S., Madhumitha, B., Maheen, Nimra, Makkouk, Khaled M., Mamedova, Sabina, Manglli, Ariana, Manoranjitham, S.K., Mar, Talita Bernardon, Matić, Slavica, Matthew, Joshua O., Maurya, A.K., Mbewe, Willard, Medina, Raul F., Meena, N.K., Moosa, Anam, Mtonga, Andrew, Muhammad, Buhari, Mulholland, Vincent, Munguía, M.E.G., Mushtaq, Sehrish, Mustafayev, Eldar, Muthu, Sakthivel, Muturi, Samuel Mwangangi, Najar, Asma, Njeru, Lucyline Kajira, Njoroge, Samuel M.C., Noris, Emanuela, Nothnagel, Thomas, Nsabiyera, V., Nusayr, Tesneem, Ochoa-Corona, F.M., Ochwo-Ssemakula, M., Olson, J.D., Pangga, Ireneo B., Pant, R.P., Parrella, Giuseppe, Paunović, Svetlana, Pereira, Paulo Roberto Valle Da Silva, Perez, C.R., Pimenta, Ricardo José Gonzaga, Pinto, Luciana Rossini, Pocsai, Emil, Poojari, Sudarsana, Portal, Orelvis, Pun, K.B., Pushpanathan, Sangeetha, Raj, Chandramani, Raj, S.K., Ramalho-Bean, T.O., Randa-Zelyut, Filiz, Relevante-Belagantol, Cherry A., Riaz, Hafsa, Rishi, Narayan, Rivera-Bustamante, R., Roumi, Vahid, Sadiq, Saleha, Saeed, Sana Tabanda, Sajid, Qurat-ul Ain, Samad, Abdul, Samah, S., Shafiq, Muhammad, Shaheen, Mohamed Nasr Fathi, Shakeel, Muhammad Taimoor, Sharma, V.K., Sharma, Susheel Kumar, Sharma, Tejaswini, Sharma, Rajan, Shevchenko, O., Shevchenko, T., Silva, Fábio Nascimento da, Silveira, A.T.L., Singh, Rachana, Singh, Shweta, Singh, M.K., Singh, Gyanendra Pratap, Sinha, Soumya, Snihur, H., Sokhandan-Bashir, Nemat, Srivastava, Ashish, Srivastava, Sonal, Sta. Cruz, Filomena C., Sánchez-Navarro, Jesús Ángel, Sukal, A., Sundararaj, Deepan, Sutula, Chet, Taglienti, Anna, Çağlayan, Kadriye, Tavares Esashika, M.L., Tawaha, Abdel Rahman M., Tegg, Robert, Thangavel, Tamilarasan, Thirumalaiappan, Priyanga, Thompson, J.R., Tiberini, Antonio, İlbağı, Havva, Tomassoli, Laura, Trucco, Verónica, Çıtır, Ahmet, Ulubaş Serçe, Çiğdem, Umar, Muhammad, Valadez-Moctezuma, E., Varma, Anupam, Vazquez-Iglesias, I., Vemulapati, Bhadra Murthy, Venkataravanappa, V., Verma, H.N., Wachira, Francis Nyamu, Wilson, Calum, Xavier, C.A.D., Xavier, A.S., Yadav, Priya, Zagrai, Luminița-Antonela, Zagrai, Ioan, Zanardo, Larissa G., Zelaya-Molina, L.X., Zhang, Shulu, Zia, Amjad, and Ziebell, Heiko

Published
2024
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