Search

Your search keyword '"Abrescia, Nicola G.A."' showing total 34 results
Start Over Author "Abrescia, Nicola G.A."
34 results on '"Abrescia, Nicola G.A."'

2. SUMOylation controls Hu antigen R posttranscriptional activity in liver cancer

Author

Lachiondo-Ortega, Sofia, Rejano-Gordillo, Claudia M., Simon, Jorge, Lopitz-Otsoa, Fernando, C. Delgado, Teresa, Mazan-Mamczarz, Krystyna, Goikoetxea-Usandizaga, Naroa, Zapata-Pavas, L. Estefanía, García-del Río, Ana, Guerra, Pietro, Peña-Sanfélix, Patricia, Hermán-Sánchez, Natalia, Al-Abdulla, Ruba, Fernandez-Rodríguez, Carmen, Azkargorta, Mikel, Velázquez-Cruz, Alejandro, Guyon, Joris, Martín, César, Zalamea, Juan Diego, Egia-Mendikute, Leire, Sanz-Parra, Arantza, Serrano-Maciá, Marina, González-Recio, Irene, Gonzalez-Lopez, Monika, Martínez-Cruz, Luis Alfonso, Pontisso, Patrizia, Aransay, Ana M., Barrio, Rosa, Sutherland, James D., Abrescia, Nicola G.A., Elortza, Félix, Lujambio, Amaia, Banales, Jesus M., Luque, Raúl M., Gahete, Manuel D., Palazón, Asís, Avila, Matias A., G. Marin, Jose J., De, Supriyo, Daubon, Thomas, Díaz-Quintana, Antonio, Díaz-Moreno, Irene, Gorospe, Myriam, Rodríguez, Manuel S., and Martínez-Chantar, María Luz

Published
2024
Full Text
View/download PDF

17. Theoretical study of a new DNA structure: The antiparallel Hoogsteen duplex

Author

Cubero, Elena, Abrescia, Nicola G.A., Subirana, Juan A., Luque, Javier, and Orozco, Modesto

Subjects
Molecular dynamics -- Research, DNA -- Structure, Chemistry
Abstract

The structure of a new form of duplex DNA, the antiparallel Hoogsteen duplex, is studied in polyd(AT) sequences by means of state-of-the-art molecular dynamics simulations in aqueous solution. The structure has many similarities with the standard Watson-Crick duplex in terms of general structure, flexibility and molecular regional patterns.

Published
2003

18. Crystal structure of an antiparallel DNA fragment with Hoogsteen base pairing

Author

Abrescia, Nicola G.A., Thompson, Andrew, Huynh-Dinh, Tam, and Subirana, Juan A.

Subjects
DNA -- Analysis, X-ray crystallography -- Methods, Structure-activity relationships (Biochemistry) -- Analysis, Science and technology
Abstract

We report here an alternative double-helical structure of the DNA molecule. It has been found in the d(ATA[sup.Br]UAT) and d(ATATAT) sequences by single-crystal x-ray crystallography. This sequence is found not only in TATA boxes, but also in other regulatory regions of DNA. Bases of the two antiparallel strands form Hoogsteen pairs, with adenines in the syn conformation. The structure is related neither to those found in triple helices nor to parallel DNA duplexes. Its conformational parameters are very similar to those of duplex DNA in the B form. Both forms may coexist under physiological conditions, although the Hoogsteen pairing greatly influences the recognition sites on DNA. Our results demonstrate that an alternative to the classical B-DNA double helix is possible.

Published
2002

21. DNA vaccination regimes against Schmallenberg virus infection in IFNAR−/− mice suggest two targets for immunization

Author

Boshra, Hani [0000-0001-9924-8126], Abrescia, Nicola G.A. [0000-0001-5559-1918], Charro, Diego [0000-0002-3197-5180], Lorenzo, Gema [0000-0003-1869-9051], Boshra, Hani, Charro, Diego, Lorenzo, Gema, Sánchez, I., Lázaro, Beatriz, Brun Torres, Alejandro, Abrescia, Nicola G.A., Boshra, Hani [0000-0001-9924-8126], Abrescia, Nicola G.A. [0000-0001-5559-1918], Charro, Diego [0000-0002-3197-5180], Lorenzo, Gema [0000-0003-1869-9051], Boshra, Hani, Charro, Diego, Lorenzo, Gema, Sánchez, I., Lázaro, Beatriz, Brun Torres, Alejandro, and Abrescia, Nicola G.A.

Abstract

Schmallenberg virus (SBV) is an RNA virus of the Bunyaviridae family, genus Orthobunyavirus that infects wild and livestock species of ruminants. While inactivated and attenuated vaccines have been shown to prevent SBV infection, little is known about their mode of immunity; specifically, which components of the virus are responsible for inducing immunological responses in the host. As previous DNA vaccination experiments on other bunyaviruses have found that glycoproteins, as well as modified (i.e. ubiquitinated) nucleoproteins (N) can confer immunity against virulent viral challenge, constructs encoding for fragments of SBV glycoproteins GN and GC, as well as ubiquitinated and non-ubiquitinated N were cloned in mammalian expression vectors, and vaccinated intramuscularly in IFNAR−/− mice. Upon viral challenge with virulent SBV, disease progression was monitored. Both the ubiquitinated and non-ubiquitinated nucleoprotein candidates elicited high titers of antibodies against SBV, but only the non-ubiquitinated candidate induced statistically significant protection of the vaccinated mice from viral challenge. Another construct encoding for a putative ectodomain of glycoprotein GC (segment aa. 678–947) also reduced the SBV-viremia in mice after SBV challenge. When compared to other experimental groups, both the nucleoprotein and GC-ectodomain vaccinated groups displayed significantly reduced viremia, as well as exhibiting no clinical signs of SBV infection. These results show that both the nucleoprotein and the putative GC-ectodomain can serve as protective immunological targets against SBV infection, highlighting that viral glycoproteins, as well as nucleoproteins are potent targets in vaccination strategies against bunyaviruses. © 2017 The Authors

Published
2017

28. X-ray and NMR studies of the DNA oligomer d(ATATAT): Hoogsteen base pairing in duplex DNA

Author

Abrescia, Nicola G.A., Gonzalez, Carlos, Gouyette, Catherine, and Subirana, Juan A.

Subjects
Nuclear magnetic resonance -- Analysis, X-ray crystallography -- Analysis, Oligomers -- Analysis, Oligomers -- Structure, DNA microarrays -- Comparative analysis, DNA microarrays -- Structure, Biological sciences, Chemistry
Abstract

The X-ray crystallography and NMR (nuclear magnetic resonance) analyzes the structure of the oligonucleotide d(ATATAT) found in two different forms. The differences between the B-form and Hoogsteen DNA are presented.

Published
2004

33. When pseudosymmetry and merohedral twinning come across: the case of the d(ApTpApTpApT) oligonucleotide in a hexagonal lattice.

Author

Abrescia, Nicola G.A. and Subirana, Juan A.

Subjects
*OLIGONUCLEOTIDES, *CRYSTALLOGRAPHY
Abstract

The d(ApTpApTpApT) deoxyhexanucleotide packs in two different lattice systems: monoclinic and hexagonal. In this study, it is shown that in the hexagonal crystals twinning and pseudosymmetry coexist. The presence of both components resulted in a puzzling space-group determination. Only when a correct model became available [Hoogsteen-DNA; Abrescia et al. (2002), Proc. Natl Acad. Sci. USA, 99, 2806-2811] was it possible to discriminate between twinning and pseudosymmetry. Here, the steps that led to a correct spacegroup assignment and to the solution of the oligonucleotide structure in the hexagonal lattice by molecular replacement are described. [ABSTRACT FROM AUTHOR]

Published
2002
Full Text
View/download PDF

34. Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR-/- mice

Author

Gabriel Soares Guerra, Diego Barriales, Gema Lorenzo, Sandra Moreno, Juan Anguita, Alejandro Brun, Nicola G.A. Abrescia, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Fundación 'la Caixa', Guerra, Gabriel Soares, Barriales, Diego, Lorenzo, Gema, Moreno, Sandra, Anguita, Juan, Brun Torres, Alejandro, and Abrescia, Nicola G.A.

Subjects
Infectious Diseases, Orthobunyavirus, General Veterinary, General Immunology and Microbiology, Cell-mediate immunity, Public Health, Environmental and Occupational Health, Molecular Medicine, Pan-vaccine, Schmallenberg virus, Subunit vaccine
Abstract

10 Pág., Schmallenberg Virus (SBV), an arbovirus from the Peribunyaviridae family and Orthobunyavirus genus, was discovered in late 2011 in Germany and has been circulating in Europe, Asia and Africa ever since. The virus causes a disease associated with ruminants that includes fever, fetal malformation, drop in milk production, diarrhoea and stillbirths, becoming a burden for small and large farms. Building on previous studies on SBV nucleoprotein (SBV-N) as a promising vaccine candidate, we have investigated the possible protein regions responsible for protection. Based on selective truncation of domains designed from the available crystal structure of the SBV-N, we identified both the N-terminal domain (N-term; Met1 – Thr133) and a smaller fragment within (C4; Met1 – Ala58) as vaccine prototypes. Two injections of the N-term and C4 polypeptides protected mice knockout for type I interferon (IFN) receptors (IFNAR-/-) challenged with virulent SBV, opposite to control groups that presented severe signs of morbidity and weight loss. Viremia analyses along with the presence of IFN-γ secreted from splenocytes re-stimulated with the N-terminal region of the protein corroborate that these two portions of SBV-N can be employed as subunit vaccines. Apart from both proteinaceous fragments being easily produced in bacterial cells, the C4 polypeptide shares a high sequence homology (∼87.1 %) with the corresponding region of nucleoproteins of several viruses of the Simbu serogroup, a group of Orthobunyaviruses that comprises SBV and veterinary pathogens like Akabane virus and human infecting viruses like Oropouche. Thus, we propose that this smaller fragment is better suited for vaccine nanoparticle formulation, and it paves the way to further research with other related Orthobunyaviruses., This work was supported by the Spanish Ministerio de Ciencia, Innovación y Universidades RTI2018-095700-B-I00 (NGAA), RTI2018-096494-B-I00 (JA), AGL2017-83226 (AB) and “La Caixa” Foundation (ID 100010434) INPhINIT “La Caixa” fellowship LCF/BQ/DI19/11730041 (GSG and NGAA).

Published
2023

Catalog

Books, media, physical & digital resources
See catalog results