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Fermented soybean grain (FSBG) is considered improper to use as a protein source in animal nutrition, since it is assumed that defects cause changes on its chemical composition and favor mycotoxins production, but chemical composition data does not support this theory and in vivo studies are missing. Thus, this study aimed to evaluate the effects of FSBG in feedlot lamb diets. For that, two types of FSBG (partially fermented and completely fermented, PFSBG and CFSBG) and one standard soybean grain (SSBG) were obtained and evaluated alone or as a component of experimental diets by in vitro and in vivo studies, where FSBG totally replaced SSBG in feedlot lamb diets, which was included in the experimental diets in 17.4% on dry matter basis as protein source. Before the studies, both soybeans were sent to a specialized laboratory where no mycotoxins were detected. As a result, lower DM and carbohydrate contents but higher crude protein, fiber, and indigestible NDF contents were measured in CFSBG than in SSBG. Furthermore, both types of FSBG showed lower digestibility in vitro dry matter (IVDMD) than SSBG when evaluated separately; however, when evaluated in experimental diets, the substitution of SSBG for FSBG did not affect IVDMD. It was also observed that FSBG also had less rumen-degradable protein than SSBG (mean 47.9 vs 86.4%). In the in vivo study, FSBG did not affect nutrient intake, apparent digestibility, or animal performance (i.e., average daily gain and carcass gain). Thus, mycotoxins-free FSBG may be an alternative to totally replace SSBG in feedlot lamb diets., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

A better understanding of the nutritional requirements of sheep, especially in terms of minerals, is crucial for improving production. We estimated the net requirements for Ca, P, K, Mg, and Na for gain (NCa g , NP g , NK g , NMg g , and NNa g ) and maintenance (NCa m , NP m , NK m , NMg m , and NNa m ) in male and female hair sheep. Six datasets with 248 individual records of hair sheep (139 non-castrated males, 75 castrated males and 34 females) were used to estimate the net macromineral requirements for gain. To estimate the net macromineral requirements for maintenance, 52 observations (26 non-castrated and 26 castrated males) were used. A meta-analytical approach was applied, using non-linear mixed effects models and the study as a random effect. Based on information criteria for model selection, heterogeneous variance functions were more likely to describe mineral requirements with a low level of model selection uncertainty. The adopted criteria allowed the choice of the best models to represent the macromineral requirements. The chosen models explained the observed variability in the sex, and the choices were based on a low level of uncertainty (w ≥ 0.90). Irrespective of sex, NCa g and NP g decreased with increasing BW from 10 to 30 kg and average daily gain (ADG) of 150 g/day, ranging from 1.71-1.38; 1.83-1.57; 1.82-1.51 of Ca and 0.86-0.66; 0.92-0.78; 0.92-0.75 of P for non-castrated males, castrated males, and females, respectively. The NK g remained constant, with mean values of 0.26 g/day. The NNa g range was 0.17 to 0.14 g/day for non-castrated males, 0.20 to 0.25 g/day for females, and constant (0.18 g/day) for castrated males with an increase in BW from 10 to 30 kg and an ADG of 150 g/day. Macromineral requirements for maintenance (mg/kg BW) and retention (%) were 23.70 and 54.30 for Ca, 25.33 and 79.80 for P, 11.74 and 5.00 for K, 2.63 and 8.50 for Mg, and 7.01 and 8.10 for Na for males. The International Committees did not provide inferences about the sex influence on mineral requirements. Our study indicates that sex is one factor that influences the macromineral requirements for gain. The information generated in this study can be used to optimize the mineral management of hair sheep in the growing phase in tropical regions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Herbster, Abreu, Brito Neto, Mendes, Silva, Marcondes, Mazza, Cabral, Bezerra, Oliveira and Pereira.)

The objective of this study was to evaluate increasing levels of inclusion of dry distillery grains (DDGs) in substitution of corn and urea in multiple supplements for beef cattle of Urochloa brizantha cv. Marandu on the consumption, digestibility, and efficiency of microbial synthesis and use of nitrogen. We used four Nellore bulls cannulated in the rumen with an average age of 24 months and 445.12 ± 34.4 kg of body weight (BW), in a 4 × 4 Latin square design. Supplements differed by the inclusion level of DDG (0%, 31.5%, 63.0%, and 94.5%). Increasing levels of DDG inclusion decreased dry matter intake (DMI) (P = 0.002), forage (P = 0.002), organic matter (OM) (P = 0.001), crude protein (CP) (P = 0.037), and total digestible nutrients (TDN) (P < 0.001) and had a quadratic effect on the intake of non-fibrous carbohydrates (NFC) (P = 0.002). It was observed an increase in the digestibility of ether extract (EE) (P = 0.005), however a decrease in the digestibility of NFC (P = 0.001). Inclusion of DDG did not influence ruminal pH. There was a quadratic effect at collection times for ruminal ammoniacal nitrogen (P < 0.05), except for the supplement with 94.5% DDG where the effect was linear (P = 0.002). Nitrogen intake was 10.9% higher when there was no DDG in the supplement, compared with the supplement with 94.5% DDG (P = 0.039). The excretion of N by feces was greater when there was a greater amount of DDG in the supplement (P = 0.027), the opposite occurred with urine excretion of N, being higher when there was less amount of DDG in the supplement (P = 0.027). Increasing levels of DDG did not affect ruminal microbial protein yield (P > 0.05). Replacing corn and urea with up to 94.5% DDGs in multiple supplements resulted in no adverse effect efficiency of microbial synthesis, although nutrient intake, total digestible nutrients, and use of nitrogen were reduced at 94.5% DDG inclusion.