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Background: Female sexual dysfunction (FSD) and the associated risk factors are less studied in the Middle East. This study is designed to determine the prevalence of FSD and its associated risk factors in Jordan. Materials and Methods: A group of women were interviewed using detailed questionnaire on several aspects of FSD including desire, arousal, lubrication, orgasm, satisfaction, and pain disorders. They underwent sociodemographic investigation. Prevalence and risk factors of FSD are calculated in different age groups. Results: A total of 470 women aged 32.6 ± 9.6 years are included in this study. Desire problems were detected in 49.4% (232/470) of the studied women, while an arousal problem in 31.9% (150/470) and lubrication problem in 39.2% (184/470). An orgasm problem was detected in 39.57% (186/470) of the studied women, while satisfaction problem was detected in 43.82% (206/470) and pain problem during sexual relation in 19.2% (90/470). There was positive significant correlation between each domain of the FSD and the studied women’s age except pain. FSD was found to be significant in women with more than four children (83.3%, P < 0.02), those who had been married for more than 10 years (76.7%, P < 0.02), in women with chronic medical diseases (76.7%, P < 0.02), in unemployed women (76.7%, P < 0.02), and in women not using contraception (75.2%, P < 0.005). There was no significant correlation with the level of education (P < 0.34) and monthly income (P < 0.24). Conclusion: The prevalence of FSD in Jordan is about 64.7%, the desire disorders are the most prevalent domain of FSD, and age is the most significant risk factor for FSD. Further research is needed with larger and more comprehensive sample to estimate the magnitude of FSD and to confirm its relationship with different risk factors. [ABSTRACT FROM AUTHOR]