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IntroductionEpilepsy is a common central nervous system disorder characterized by abnormal brain electrical activity. We aimed to compare the metabolic profiles of plasma from patients with epilepsy across different etiologies, seizure frequency, seizure type, and patient age to try to identify common disrupted pathways.Material and methodsWe used data from three separate cohorts. The first cohort (PED-C) consisted of 31 pediatric patients with suspicion of a genetic disorder with unclear etiology; the second cohort (AD-C) consisted of 250 adults from the Estonian Biobank (EstBB), and the third cohort consisted of 583 adults ≥ 69 years of age from the EstBB (ELD-C). We compared untargeted metabolomics and lipidomics data between individuals with and without epilepsy in each cohort.ResultsIn the PED-C, significant alterations (p-value ConclusionOur results suggest that cell membrane fluidity may have a significant role in the mechanism of epilepsy, and changes in lipid balance may indicate epilepsy. However, further studies are needed to evaluate whether untargeted metabolomics analysis could prove helpful in diagnosing epilepsy earlier.

The number of people living in rural areas of low and middle-income countries is projected to increase in the coming decades. It is in the rural areas of these countries where a large majority of the world’s extreme poor reside. The livelihoods of two to three billion rural people depend on small farms. These small farms are responsible for the production and supply of a large portion of the calories feeding low- and middle-income countries. Small farms are also preservers of crops and associated biodiversity and with the right incentives can contribute to land stewardship. Small farms are diverse, and, hence, so are their associated challenges. We categorize small farms as commercial farms, small farms in transition and subsistence-oriented farms and highlight evidence-based innovations for the sustainable transformation of each type of small farm. Broadly, small farms face high transaction costs, lack collective action, and experience coordination failure in production and marketing. Lack of market access is also a major challenge. Investments in infrastructure, including those that support access to digital technologies, can improve farmers’ access to markets and incentives as well as foster growth in the midstream segments of the value chain that provide inputs, storage, processing, and logistics to small farms. Rural Non-Farm Employment (RNFE) is increasingly the main source of income for most small farmers and provides them with a risk diversification strategy and cash, both to purchase food and for farm investments to raise productivity, expand commercial activities, and produce higher-value products. Public investments and policies that facilitate growth of the agrifood system must pay more attention to creating enabling environments for the development of RNFE and strengthening the synergy between agriculture and RNFE in rural areas.

Metabolism is a highly regulated process that provides nutrients to cells and essential building blocks for the synthesis of protein, DNA and other macromolecules. In healthy biological systems, metabolism maintains a steady state in which the concentrations of metabolites are relatively constant yet are subject to metabolic demands and environmental stimuli. Rare genetic disorders, such as inborn errors of metabolism (IEM), cause defects in regulatory enzymes or proteins leading to metabolic pathway disruption and metabolite accumulation or deficiency. Traditionally, the laboratory diagnosis of IEMs has been limited to analytical methods that target specific metabolites such as amino acids and acyl carnitines. This approach is effective as a screening method for the most common IEM disorders but lacks the comprehensive coverage of metabolites that is necessary to identify rare disorders that present with nonspecific clinical symptoms. Fortunately, advancements in technology and data analytics has introduced a new field of study called metabolomics which has allowed scientists to perform comprehensive metabolite profiling of biological systems to provide insight into mechanism of action and gene function. Since metabolomics seeks to measure all small molecule metabolites in a biological specimen, it provides an innovative approach to evaluating disease in patients with rare genetic disorders. In this review we provide insight into the appropriate application of metabolomics in clinical settings. We discuss the advantages and limitations of the method and provide details related to the technology, data analytics and statistical modeling required for metabolomic profiling of patients with IEMs.

Simple Summary A tracheal lavage is commonly used to characterize the microbes that may be causing pneumonia in sea turtles, typically by culture-dependent methods. In this study, we characterized the tracheal lavage microbiome through culture-independent methods and compared the resulting sequence data to conventional cultures, the degree of radiographic lung abnormalities, and pathogens of sea turtles as previously reported in the literature. This study also evaluates the microbial communities at different sections of the respiratory tract from deceased sea turtles. We found that radiographic lung abnormalities do not correlate with the tracheal lavage microbiome, tracheal lavage cultures under-represent the microbial community as determined by culture-independent methods, many previously reported sea turtle pathogens are present in low abundance of the tracheal lavage microbiome, and tracheal lavages are not representative of other sections of the respiratory tract. Abstract Respiratory disease is a common cause of morbidity and mortality in sea turtles, including the Kemp’s ridley sea turtle (Lepidochelys kempii). Although culture-dependent methods are typically used to characterize microbes associated with pneumonia and to determine treatment, culture-independent methods can provide a deeper understanding of the respiratory microbial communities and lead to a more accurate diagnosis. In this study, we characterized the tracheal lavage microbiome from cold-stunned Kemp’s ridley sea turtles at three time points during rehabilitation (intake, rehabilitation, and convalescence) by analyzing the 16S rRNA gene collected from tracheal lavage samples. We retrospectively developed a radiographic scoring system to grade the severity of lung abnormalities in these turtles and found no differences in diversity or composition of microbial communities based on radiographic score. We also found that the culture isolates from tracheal lavage samples, as well as other previously reported sea turtle pathogens, were present in variable abundance across sequenced samples. In addition to the tracheal microbial community of live turtles, we characterized microbial communities from other segments of the respiratory tract (glottis, trachea, anterior lung, posterior lung) from deceased turtles. We found a high degree of variability within turtles and a high degree of dissimilarity between different segments of the respiratory tract and the tracheal lavage collected from the same turtle. In summary, we found that the pulmonary microbial community associated with pneumonia in sea turtles is complex and does not correlate well with the microbial community as identified by tracheal lavage. These results underscore the limitations of using tracheal lavage for identification of the causative agents of pneumonia in sea turtles.

Simple Summary Kemp’s ridley turtle is the rarest species of sea turtle and is considered to be critically endangered. While the main habitat of adults is the Gulf of Mexico, juveniles forage off the northeastern United States during summer. However, when juveniles fail to leave this area in autumn before water temperatures fall below 10 °C, they become cold-stunned and strand on beaches. Every winter, there are coordinated efforts to rescue and rehabilitate these stranded sea turtles. Cold-stunned sea turtles require supportive care because they suffer from a variety of medical problems, including steatitis, or inflammation of fat tissue. The goal of this study was to further understand steatitis by investigating blood analytes involved with vitamin E metabolism, and investigating microscopic changes that occur in affected fat tissue. We found that various contributing factors may play a role in the development of steatitis. Based on these findings, we conclude that enhanced vitamin E supplementation and dietary modification during rehabilitation may be useful in preventing and treating steatitis. The results from this study will contribute to improving rehabilitation outcomes and successful release of this endangered sea turtle species. Abstract The pathogenesis of steatitis that infrequently occurs in cold-stunned Kemp’s ridley sea turtles (KRT; Lepidochelys kempii) has been undetermined. The objectives of this study were to investigate the clinical (n = 23) and histologic findings (n = 11) in cold-stunned KRT, and to compare plasma concentrations of α-tocopherol (vitamin E), thiobarbituric acid reactive substances (TBARS), and the TBARS to vitamin E (T/E) ratio (an assessment of oxidative stress) between cold-stunned KRT with clinically and/or histologically confirmed steatitis (n = 10) and free-ranging KRT (n = 9). None of the cold-stunned turtles had clinically detectable steatitis at admission, and the median number of days to diagnosis of steatitis was 71 (range 33–469). Histologic findings of affected adipose tissue included heterophilic (n = 9) and/or histiocytic (n = 5) steatitis, fat necrosis (n = 7), myonecrosis (n = 2), and intralesional bacteria (n = 6). Cold-stunned KRT had significantly lower plasma vitamin E concentrations (median = 3.5 nmol/g), lower plasma TBARS concentrations (median = 1.6 nmol/g), and higher T/E ratios (median = 0.37), than controls (208.8 nmol/g; 2.1 nmol/g; 0.01, respectively). These results suggest a multifactorial etiology for the development of steatitis in KRT during rehabilitation, including tissue injury, septicemia, and various factors resulting in imbalances of anti-/oxidative status. By highlighting the need to provide more effective vitamin E supplementation, and the need to re-assess specific components of the diet, this study may lead to reduced incidence and improved medical management of steatitis in cold-stunned sea turtles.

Oxytetracycline (42 mg/kg SC q6d) was injected into the dorsal shoulder region of 29 sea turtles during clinical management after stranding, including 10 Kemp's ridley (Lepidochelys kempii), 10 loggerhead (Caretta caretta), and nine green sea turtles (Chelonia mydas). Before injection, the drug was diluted into a suitable fluid for injection (5–20 ml/kg) as chosen by the attending veterinarian. Turtles were treated for as long as clinically indicated prior to discontinuing the drug (range 2–10 doses, median four). After the final injection, blood was collected at 1, 2, 4, 8, 72, 144, and 288 h and plasma harvested for measurement of the oxytetracycline plasma concentration. To limit the volume of blood collected, a sparse sampling strategy was used with samples collected at only three or four time points per turtle. The plasma oxytetracycline concentration was measured with high pressure liquid chromatography. Population pharmacokinetics using nonlinear mixed effects modeling produced the following results: half-life, 36 h; volume of distribution, 0.78 L/kg; and area-under-the-curve, 2,799 µg*h/ml. Using this dose and method of administration, the plasma oxytetracycline concentrations were maintained above a minimum inhibitory concentration value of approximately 4 µg/ml for approximately 6 days.

As the COVID-19 pandemic began spreading globally in March, national governments and the international donor community mobilized quickly to cushion the impact. The speed, uncertainty, and potential severity of the crisis meant that many disbursement approvals occurred faster than usual, and it was difficult to gain a clear picture of where money was going, how different donors were targeting their resources, and how effectively such resources were being utilized. Many governments also began establishing their own funding baskets to respond to COVID-19, often by encouraging donations from politicians, celebrities, businesspeople, and everyday citizens, sometimes without full transparency.

SynopsisMany juvenile Kemp’s ridley (Lepidochelys kempii) and loggerhead (Caretta caretta) turtles strand during fall on the beaches of Cape Cod (MA, USA), with total stranding numbers sometimes exceeding 300 turtles per year. Once rehabilitated, turtles must be released at beaches with appropriate water temperatures, often requiring transportation to southeastern coastal states of the USA. These transportation events (transports) may approach or exceed 24 h in duration. Kemp’s ridley turtles are known to exhibit an adrenal stress response during such transports, but the effect of transport duration has been unclear, and no other sea turtle species has been investigated. To assess whether transport duration and/or species affects physiological reactions to transport, we studied pre- and post-transport physiological measures in Kemp’s ridley and loggerhead turtles transported by ground for

Since the 1980s, many developing countries have introduced policies to promote seed industry growth and improve the delivery of modern science to farmers, often with a long-term goal of increasing agricultural productivity in smallholder farming systems. Public, private, and civil society actors involved in shaping policy designs have, in turn, developed competing narratives around how best to build an innovative and sustainable seed system, each with varying goals, values, and levels of influence. Efforts to strike a balance between these narratives have often played out in passionate discourses surrounding seed rules and regulations. As a result, however, policymakers in many countries have expressed impatience with the slow progress on enhancing the contribution of a modern seed industry to the overarching goal of increasing agricultural productivity growth. One reason for this slow progress may be that policymakers are insufficiently cognizant of the trade-offs associated with rules and regulations required to effectively govern a modern seed industry. This suggests the need for new data and analysis to improve the understanding of how seed systems function. This paper explores these issues in the context of Asia's rapidly growing seed industry, with illustrations from seed markets for maize and several other crops, to highlight current gaps in the metrics used to analyze performance, competition, and innovation. The paper provides a finite set of indicators to inform policymaking on seed system design and monitoring, and explores how these indicators can be used to inform current policy debates in the region., Highlights • Despite several decades of policy reforms, decisionmaking on seed systems in many developing countries remain poorly informed by market- and policy-relevant indicators. • We explores Asia’s rapidly growing seed industry to illustrate current gaps in metrics used to analyze performance, competition, and innovation. • We present a finite set of seed system indicators to inform policymaking. • We discuss how these indicators might be used to shape competing narratives and inform current policy discourse around seed system develop in the region.

Sea turtle rehabilitation clinics and aquaria frequently transport stranded sea turtles long distances out of water, e.g. for release at sites with appropriate water temperatures. Endangered Kemp's ridley turtles (Lepidochelys kempii) are known to exhibit an adrenal stress response during such transports. In an opportunistic study of turtles transported by road from Massachusetts to Georgia for release, we tested whether placing turtles in saltwater pools for short periods after transport would help turtles recover from transport-related stress. Eighteen juvenile Kemp's ridley turtles were examined and blood samples collected (1) immediately pre-transport, (2) immediately post-transport and (3) after a 6 h (n = 9) or 24 h (n = 9) post-transport period in unfamiliar pools, after which all turtles were released to the sea. Blood samples were analyzed for corticosterone, glucose, total white blood cell (WBC) count, heterophil/lymphocyte (H/L) ratio, pH, pO2, pCO2, HCO3 (bicarbonate), sodium, potassium, ionized calcium, lactate and hematocrit. Though the majority of turtles remained in good clinical condition, corticosterone, glucose, WBC and H/L elevated significantly during transport, while potassium declined slightly but significantly. After at least 6 h in a saltwater pool, potassium and glucose returned to pre-transport baselines and corticosterone partially recovered toward baseline. Extending the pool time to 24 h did not markedly enhance the physiological recovery of turtles, and two immune measures (WBC, H/L) remained elevated from the effect of transport. Six hours in a saltwater pool appears to facilitate the recovery of Kemp's ridley sea turtles from transport-related stress and may therefore improve their readiness for release.

type="main" xml:id="dpr12143-abs-0001"> Export bans have been frequently used by developing countries in recent years in an attempt to ensure domestic food supplies and insulate domestic market prices from international price hikes. This article uses Tanzania to examine the impact of export bans using a computable general equilibrium model. We find that banning cross-border maize exports has very little effect on the national food price index and that the benefits from lower maize prices are captured primarily by urban households, while maize producer prices decrease significantly. The export ban further decreases the wage rate for low-skilled labour and the returns to land, while returns to non-agricultural capital and wage rates for skilled labour increase, further hurting poor rural households and thus increasing poverty for the country as a whole.

A survey of sea turtle rehabilitation facilities in the United States revealed that 34 facilities released 11,417 sea turtles through 2016. The number of turtles released per time period i...

Iohexol clearance testing was used to determine glomerular filtration rates (GFR) for 12 juvenile Kemp's ridley turtles (Lepidochelys kempii) that stranded because of cold-stunning. Iohexol was injected intravenously at 75 mg/kg, with subsequent plasma collection at 4, 8, and 24 h post-injection for measurement of iohexol iodine concentrations by inductively coupled plasma mass spectrometry (ICP-MS). Initial GFR was determined on median day 4 of hospitalization (range day 2–5) at an average body temperature of 19°C (66°F) (range 18–21°C [64–70°F]). Mean initial GFR was 4.6 mL/kg/h (range 1.9–8.7 mL/kg/h, n = 10). Convalescent GFR was determined on median day 126 of hospitalization (range day 108–232) at an average body temperature of 24°C (75°F) (range 23–25°C [74–77°F]). Mean convalescent GFR was 13.5 mL/kg/h (range 8.5–21.5 mL/kg/h, n = 10). Statistical analysis of paired initial and convalescent data demonstrated a significantly lower initial GFR, body weight, straight carapace length, and bod...

Objective—To assess selected clinicopathologic variables at hospital admission (day 1) for cold-stunned Kemp's ridley turtles (Lepidochelys kempii) that died during the first 3 days after admission (nonsurvivors) and turtles that survived (survivors) and to determine the percentage change of each variable from day 1 to day of death (nonsurvivors) or to day 2 or 3 of hospitalization (survivors). Design—Retrospective case-control study. Animals—64 stranded, cold-stunned Kemp's ridley turtles hospitalized from October 2005 through December 2009. Procedures—Blood gas, pH, Hct, and selected biochemical values in blood samples determined on day 1 and day of death (nonsurvivors; n = 32) or day 2 or 3 of hospitalization (survivors; 32) were obtained from medical records. For each variable, initial values and percentage changes (from initial values to values at the day of death or day 2 or 3 of hospitalization) were compared between survivors and nonsurvivors. Results—Compared with blood analysis findings for survivors, nonsurvivors initially had significantly higher potassium concentration and Pco2 and significantly lower Po2, pH, and bicarbonate concentration than did survivors. For the first 2 or 3 days of hospitalization, percentage changes in potassium, lactate, and ionized calcium concentrations were significantly higher and percentage changes in pH and plasma glucose and bicarbonate concentrations were significantly lower in nonsurvivors. Conclusions and Clinical Relevance—At hospital admission, cold-stunned Kemp's ridley turtles were affected by metabolic and respiratory derangements; severe derangements were associated with death. Evaluation of blood gas, pH, Hct, and selected clinicopathologic variables provided useful clinical and prognostic information during rehabilitation of cold-stunned Kemp's ridley turtles.

The livestock is an important sub-sector within Ethiopia’s economy in terms of its contributions to both agricultural value-added and national GDP. Between 1995/96 and 2005/06, the livestock sub-sector’s share averaged 24 percent of agricultural GDP and 11 percent of national GDP, with the highest shares recorded at 27 percent and 13 percent, respectively, at its peak (NBE 2005/06). The contribution of livestock and livestock product exports to foreign exchange earnings is also large. The annual average revenue from livestock and livestock product exports was estimated to be 13 percent of the annual national foreign exchange earnings during the period 2000/01 to 2007/08 (NBE 2007/08). Given the large porous border, a large amount of cross-border exports also go un-recorded. Therefore, the official estimates of foreign exchange earnings do not necessarily reflect the actual volume of exports.At the household level, livestock plays a critical economic and social role in the lives of pastoralists, agro-pastoralists, and smallholder farm households. Livestock fulfills an important function in coping with shocks, accumulating wealth, and serving as a store of value in the absence of formal financial institutions and other missing markets. In the case of smallholder mixed farming systems, livestock provides nutritious food, additional emergency and cash income, transportation, farm outputs and inputs, and fuels for cooking food. In the case of pastoralists, livestock represents a sole means to support and sustain their livelihoods. Furthermore, available research suggests that with economic growth, consumption patterns tend to change towards high value and high protein foods, such as those derived from livestock (Delgado et al. 1999). This implies that, given the economic growth in Ethiopia and the region, the market demand for livestock and livestock products is likely to continue growing in the future. The government recognizes the importance of livestock in poverty alleviation and has increased its emphasis on modernizing and commercializing the livestock sub-sector in recent years (SPS-LMM 2008).Focusing on four key livestock classes—cattle, sheep, goats, and chicken—this chapter undertakes three tasks: (1) it provides a characterization of the livestock subsector, (2) it assesses livestock and livestock product value chains based on primary data, and (3) analyzes the trends in marketing and trade of live animals and animal products. Carrying out these tasks relies on the critical review of existing literature and policy documents, as well as extensive use of both secondary and primary data, including household and traders’ surveys. The paper is organized following the sequence of these major tasks and concludes with a summary and policy implications.

Anecdotal evidence suggests that temporal and environmental factors may contribute to the variance in the volume of pediatric orthopaedic injuries (POI), and knowing the effect of these factors could help in the allocation of resources and personnel in pediatric emergency rooms. All POI presenting to a level 1 pediatric emergency department over a 3-year period were reviewed. Environmental data, including minimal, maximal, and average daily temperatures; amount of precipitation; and lunar phase, were obtained for the study region, as were day of the week and month and season of the year. Multiple logistic regression determined which variable or combination of variables might affect the rate of POI. In the 6770 POI seen over the study period (average 6.2 per day), the day of the week and lunar phase had no effect on the rate of POI, the presence of precipitation lowered the rate slightly, and temperature had a dramatic effect, with the highest number of injuries occurring in the average daily temperature range of 70° to 79°. Winter months of November through February had the lowest rates of POI, while May had the highest.

An in-depth quantitative analysis is undertaken in this paper to assist the Southern African Development Community (SADC) Secretariat, member countries, and development partners in setting future regional investment priorities for agricultural research and development in the SADC region. A primary goal of this work was to identify a range of agricultural research priorities for achieving sector productivity and overall economic growth in southern Africa, at both the country and regional levels. This is accomplished by adopting an integrated modeling framework that combines a disaggregated spatial analytical model with an economy-wide multimarket model developed specifically for the region.

Export bans have been frequently used by developing countries in recent years in an attempt to ensure domestic food supplies and insulate domestic market prices from international price hikes. This article uses Tanzania to examine the impact of export bans using a computable general equilibrium model. We find that banning cross‐border maize exports has very little effect on the national food price index and that the benefits from lower maize prices are captured primarily by urban households, while maize producer prices decrease significantly. The export ban further decreases the wage rate for low‐skilled labour and the returns to land, while returns to non‐agricultural capital and wage rates for skilled labour increase, further hurting poor rural households and thus increasing poverty for the country as a whole.

It is widely agreed that reducing poverty in Africa south of the Sahara (SSA) depends largely on stimulating growth in agriculture. To this end, heads of state in Africa rallied to form the pan-African Comprehensive African Agriculture Development Programme (CAADP) with the goal of raising investments and improving strategy implementation. However, while implementing an agricultural agenda under the CAADP framework, more and more countries have realized that increasing public investment in agriculture alone is not enough. Policy can play an important role not only to make public investment more efficient, but also is crucial for incentivizing private sector and farmer investment in agriculture. Against this backdrop this paper takes stock of current agricultural policies in SSA with a view to identifying policies that are working as well as areas for improvement. The paper examines policies to encourage the adoption of agricultural inputs, initiate greater private-sector investment in agriculture and agro-industries, and manage price volatility while encouraging openness. The paper further reviews successful land tenure policies and property rights systems, reviews the evidence on the synergies between agriculture and nutrition, and examines how CAADP is laying the institutional architecture for improved policy formulation in Africa. In general, the paper finds that although substantial progress has been made, there is considerable scope for improvement. This is not surprising given the relatively primitive and deeply rooted nature of smallholder farming in Africa. Evidence synthesized in the paper supports the view that most policies cannot be implemented in isolation. Rather, policies tend to be most effective when implemented along with complementary policies and public investments.

Juvenile Kemp’s ridley sea turtles were studied before and after ground transportation to assess potential transportation stress. Turtles appeared mildly stressed after a 13h transport (mild elevations in glucose) and moderately stressed by a 26h transport (more pronounced elevations in corticosterone and glucose), but remained in good clinical health., Sea turtle rehabilitation centres frequently transport sea turtles for long distances to move animals between centres or to release them at beaches, yet there is little information on the possible effects of transportation-related stress (‘transport stress’) on sea turtles. To assess whether transport stress is a clinically relevant concern for endangered Kemp’s ridley sea turtles (Lepidochelys kempii), we obtained pre-transport and post-transport plasma samples from 26 juvenile Kemp’s ridley sea turtles that were transported for 13 h (n = 15 turtles) or 26 h (n = 11 turtles) by truck for release at beaches. To control for effects of handling, food restriction and time of day, the same turtles were also studied on ‘control days’ 2 weeks prior to transport, i.e. with two samples taken to mimic pre-transport and post-transport timing, but without transportation. Blood samples were analysed for nine clinical health measures (pH, pCO2, pO2, HCO3, sodium, potassium, ionized calcium, lactate and haematocrit) and four ‘stress-associated’ parameters (corticosterone, glucose, white blood cell count and heterophil-to-lymphocyte ratio). Vital signs (heart rate, respiratory rate and cloacal temperature) were also monitored. Corticosterone and glucose showed pronounced elevations due specifically to transportation; for corticosterone, this elevation was significant only for the longer transport duration, whereas glucose increased significantly after both transport durations. However, clinical health measures and vital signs showed minimal or no changes in response to any sampling event (with or without transport), and all turtles appeared to be in good clinical health after both transport durations. Thus, transportation elicits a mild, but detectable, adrenal stress response that is more pronounced during longer durations of transport; nonetheless, Kemp’s ridley sea turtles can tolerate ground transportation of up to 26 h in good health. These results are likely to depend on specific transportation and handling protocols.

The objectives of the Low Cost Microsensors (LCMS) Program are twofold. The first is to develop and deliver a long-range infrared (IR) sensor built upon an uncooled vanadium oxide (VOx) 640 X 512 format focal plane array (FPA) engine. The second is to develop an expendable microsensor built upon a VOx 160 X 128 format FPA engine. The 640 X 480 sensor is applicable to long-range surveillance and targeting missions and is a reusable asset. The 160 X 120 sensor is designed for applications where miniaturization is required as well as low cost and low power. The 160 X 120 is also intended for expendable military applications. The intent of this DUS&T effort is to further reduce the cost, weight, and power of uncooled IR sensors, and to increase the capability of these sensors, thereby expanding their applicability to military and commercial markets never before addressed by thermal imaging.

Glomerular filtration rates (GFR) were determined with the use of iohexol clearance testing for 17 clinically healthy, captive, juvenile Kemp's ridley turtles (Lepidochelys kempii) and three Kemp's ridley turtles with histopathologically confirmed renal disease. Iohexol was administered intravenously at 75 mg/kg, and blood was collected 4, 8, and 24 h after injection. Plasma iohexol concentrations were determined by high-performance liquid chromatography, and GFR was calculated. Iohexol clearance rates for healthy turtles were 9–17.4 ml/kg/h (mean: 12.6, median: 13.2, standard deviation: 2.17), and those for renal disease turtles were 10.2, 3.0, and 9.2 ml/kg/h, respectively. Administration of intravenous iohexol to Kemp's ridley turtles appeared to be safe, as no adverse effects were detected. This study provides the first data for iohexol clearance in a chelonian species, and the first GFR data for Kemp's ridley turtles. Although the data are limited, this study suggests that iohexol clearance ...