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Increasing evidence on environmental and economic benefits has raised farmers' interest in adopting alternative, less intensive soil management practices. To evaluate the influence of weed-competitive ability in response to a different tillage regime, a field study was conducted in maize under humid Central European climatic conditions in Slovenia. This study was established as a split-plot arrangement with three tillage practices (TPs) as the main plot: conventional (CN), conservation (CS), and no tillage (NT); this was combined with glyphosate application and the weed removal timing as the subplot. The weed removal timings were at the V3, V6, V9, V15, and R1 maize stages, with weed-free and weed season-long monitoring. The beginning and the end of the critical period of weed control (CPWC), based on a 5% maize yield loss rate, were determined by fitting the four-parameter log-logistic equations to the relative maize dry grain yield. The weed dry biomass from maize germination until the R1 growth stage in the NT TP was consistently lower than that in the CN and CS TP. Moreover, the NT TP resulted in a shorter CPWC (39 days after emergence (DAE)) compared to the CN (57 DAE) and CS (58 DAE). The results of CTWR (critical timing of weed removal) showed that less intensive tillage operations in the CS resulted in an earlier need for weed control (V2 and 23 DAE) compared to the CN (V3 and 39 DAE) and NT (V3 and 40 DAE). Our study suggests that the intensive tillage operations performed in the CN TP and the pre-sowing use of non-selective burndown herbicide in the NT delay the CTWR by more than 2 weeks, thus reducing the need for early post-emergence herbicide application in maize. [ABSTRACT FROM AUTHOR]