31 results on '"Adams, Damian H."'
Search Results
2. Self-reported mental health status of donor sperm-conceived adults
- Author
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Adams, Damian H., primary, Gerace, Adam, additional, Davies, Michael J., additional, and de Lacey, Sheryl, additional
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- 2021
- Full Text
- View/download PDF
3. Systemic Delivery of Anti-Integrin αL Antibodies Reduces Early Macrophage Recruitment, Inflammation, and Scar Formation in Murine Burn Wounds
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Strudwick, Xanthe L., primary, Adams, Damian H., additional, Pyne, Natasha T., additional, Samuel, Michael S., additional, Murray, Rachael Z., additional, and Cowin, Allison J., additional
- Published
- 2020
- Full Text
- View/download PDF
4. Self-reported mental health status of donor sperm-conceived adults.
- Author
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Adams, Damian H., Gerace, Adam, Davies, Michael J., and de Lacey, Sheryl
- Subjects
SPERM donation ,PANIC attacks ,MENTAL health ,PREGNANCY ,MATERNAL health - Abstract
While donor-conceived children have similar mental health outcomes compared to spontaneously conceived children, there is an inconsistency between studies investigating mental health outcomes of donor-conceived people in adulthood. This study is an online health survey that was completed by 272 donor sperm-conceived adults and 877 spontaneously conceived adults from around the world. Donor sperm-conceived adults had increased diagnoses of attention deficit disorder (P = 0.004), and autism (P = 0.044) in comparison to those conceived spontaneously. Donor sperm-conceived adults self-reported increased incidences of seeing a mental health professional (P < 0.001), identity formation problems (P < 0.001), learning difficulties (P < 0.001), panic attacks (P = 0.038), recurrent nightmares (sperm P = 0.038), and alcohol/drug dependency (P = 0.037). DASS-21 analysis revealed that donor sperm-conceived adults were also more stressed than those conceived spontaneously (P = 0.013). Both donor sperm and spontaneously conceived cohorts were matched for sex, age, height, alcohol consumption, smoking, exercise, own fertility, and maternal smoking. The increase in adverse mental health outcomes is consistent with some studies of donor-conceived adult mental health outcomes. These results are also consistent with the Developmental Origins of Health and Disease (DOHaD) phenomenon that has linked adverse perinatal outcomes, which have been observed in donor-conceived neonates, to increased risk of chronic disease, including mental health. Further work is required to reconcile our observations in adults to contrary observations reported in donor-conceived children. [ABSTRACT FROM AUTHOR]
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- 2022
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- View/download PDF
5. Self-reported physical health status of donor sperm-conceived adults
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Adams, Damian H., primary, Gerace, Adam, additional, Davies, Michael J., additional, and de Lacey, Sheryl, additional
- Published
- 2020
- Full Text
- View/download PDF
6. Self-reported physical health status of donor sperm-conceived adults.
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Adams, Damian H., Gerace, Adam, Davies, Michael J., and de Lacey, Sheryl
- Subjects
SPERM donation ,HUMAN fertility ,PREMATURE labor ,LOW birth weight ,FETAL growth retardation - Abstract
Donor-conceived neonates have poorer birth outcomes, including low birth weight and preterm delivery that are associated with poorer long-term health in adulthood through the developmental origins of health and disease (DOHaD) theory. The aim of this study was to conduct the first investigation of the adult health outcomes of donor-conceived people. An online health survey was completed by 272 donor sperm-conceived adults and 877 spontaneously conceived adults from around the world. Donor and spontaneously conceived groups were matched for age, sex, height, smoking, alcohol consumption, exercise, own fertility and maternal smoking. Donor sperm-conceived adults had significantly higher reports of being diagnosed with type 1 diabetes (P = 0.031), thyroid disease (P = 0.031), acute bronchitis (P = 0.008), environmental allergies (P = 0.046), sleep apnoea (P = 0.037) and having ear tubes/grommets surgically implanted (P = 0.046). This is the first study to investigate the health outcomes of adult donor sperm-conceived people. Donor sperm-conceived adults self-reported elevated frequencies of various health conditions. The outcomes are consistent with birth defect data from donor sperm treatment and are consistent with the DOHaD linking perturbed early growth and chronic disease in adulthood. [ABSTRACT FROM AUTHOR]
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- 2021
- Full Text
- View/download PDF
7. Data on keratin expression in human cells cultured with Australian native plant extracts
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Adams, Damian H., primary, Shou, Qingyao, additional, Wohlmuth, Hans, additional, and Cowin, Allison J., additional
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- 2016
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8. Native Australian plant extracts differentially induce Collagen I and Collagen III in vitro and could be important targets for the development of new wound healing therapies
- Author
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Adams, Damian H., primary, Shou, Qingyao, additional, Wohlmuth, Hans, additional, and Cowin, Allison J., additional
- Published
- 2016
- Full Text
- View/download PDF
9. In vivo delivery of functional Flightless I siRNA using layer-by-layer polymer surface modification
- Author
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Martens, Penny J, primary, Ly, Mai, additional, Adams, Damian H, additional, Penzkover, Kathryn R, additional, Strudwick, Xanthe, additional, Cowin, Allison J, additional, and Poole-Warren, Laura A, additional
- Published
- 2015
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10. A Novel Murine Model of Hypertrophic Scarring Using Subcutaneous Infusion of Bleomycin
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Cameron, Alexander M., primary, Adams, Damian H., additional, Greenwood, John E., additional, Anderson, Peter J., additional, and Cowin, Allison J., additional
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- 2014
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11. Mouse Strains for the Ubiquitous or Conditional Overexpression of the Flii Gene
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Thomsen, Nicole, Chappell, Anna, Ali, Radiya, Jones, Tamsin, Adams, Damian H, Matthaei, Klaus, Campbell, Hugh, Cowin, Allison J, Arkell, Ruth, Thomsen, Nicole, Chappell, Anna, Ali, Radiya, Jones, Tamsin, Adams, Damian H, Matthaei, Klaus, Campbell, Hugh, Cowin, Allison J, and Arkell, Ruth
- Abstract
The gelsolin related actin binding protein, Flii, is able to regulate wound healing; mice with decreased Flii expression show improved wound healing whereas mice with elevated Flii expression exhibit impaired wound healing. In both mice and humans Flii expression increases with age and amelioration of FLII activity represents a possible therapeutic strategy for improved wound healing in humans. Despite analysis of Flii function in a variety of organisms we know little of the molecular mechanisms underlying Flii action. Two new murine alleles of Flii have been produced to drive constitutive or tissue-specific expression of Flii. Each strain is able to rescue the embryonic lethality associated with a Flii null allele and to impair wound healing. These strains provide valuable resources for ongoing investigation of Flii function in a variety of biological processes. genesis 49:681-688, 2011.
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- 2011
12. Tropomyosin Regulates Cell Migration during Skin Wound Healing
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Lees, Justin G., primary, Ching, Yu Wooi, additional, Adams, Damian H., additional, Bach, Cuc T.T., additional, Samuel, Michael S., additional, Kee, Anthony J., additional, Hardeman, Edna C., additional, Gunning, Peter, additional, Cowin, Allison J., additional, and O'Neill, Geraldine M., additional
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- 2013
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13. Flightless I deficiency enhances wound repair by increasing cell migration and proliferation
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Cowin, Allison, Adams, Damian H, Strudwick, Xanthe L, Chan, Huater (Walter), Hooper Jones, Jane, Sander, Guy R, Rayner, T E, Matthaei, Klaus, Powell, Barry C, Campbell, Hugh, Cowin, Allison, Adams, Damian H, Strudwick, Xanthe L, Chan, Huater (Walter), Hooper Jones, Jane, Sander, Guy R, Rayner, T E, Matthaei, Klaus, Powell, Barry C, and Campbell, Hugh
- Abstract
Wound healing disorders are a therapeutic problem of increasing clinical importance involving substantial morbidity, mortality, and rising health costs. Our studies investigating flightless I (FliI), a highly conserved actin-remodelling protein, now reveal that FliI is an important regulator of wound repair whose manipulation may lead to enhanced wound outcomes. We demonstrate that FliI-deficient +/- mice are characterized by improved wound healing with increased epithetial migration and enhanced wound contraction. In contrast, FliI-overexpressing mice have significantly impaired wound healing with larger less contracted wounds and reduced cellular proliferation. We show that FliI is secreted in response to wounding and that topical application of antibodies raised against the leucinerich repeat domain of the FliI protein (FliL) significantly improves wound repair. These studies reveal that FliI affects wound repair via mechanisms involving cell migration and proliferation and that FliI might represent an effective novel therapeutic factor to improve conditions in which wound healing is impaired.
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- 2007
14. Does the removal of anonymity reduce sperm donors in Australia?
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Adams, Damian H., Ullah, Shahid, and de Lacey, Sheryl
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LEGAL status of sperm donors ,ANONYMITY ,MEDICAL laws ,MEDICAL ethics ,ORGAN donors ,POLICY sciences ,PRIVACY ,SEMEN - Abstract
The National Health and Medical Research Council's guidelines implemented in 2005 removed a sperm donor's ability to remain anonymous in every Australian State except Victoria, which had removed anonymity completely by 1998. To assess the impact of these changes on sperm donor numbers in Australia, Assisted Reproductive Technology clinics were surveyed to obtain sperm donation figures between 2000 and 2012, with additional data collected from State-based oversight groups. There was an increase in total sperm donor numbers over the study period, including the year anonymity was removed as well as the non-anonymous period. Variations in total donor numbers and numbers of new recruits observed during the period could not be attributed to any specific change in policy or practice. As total sperm donor numbers have been increasing, the removal of a donor's ability to remain anonymous has not been detrimental to the availability of sperm donors in Australia. [ABSTRACT FROM AUTHOR]
- Published
- 2016
15. Gamete donor medical records: whose information is it?
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Adams, Damian H, primary
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- 2012
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16. Flii neutralizing antibodies improve wound healing in porcine preclinical studies
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Jackson, Jessica E., primary, Kopecki, Zlatko, additional, Adams, Damian H., additional, and Cowin, Allison J., additional
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- 2012
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17. Mouse strains for the ubiquitous or conditional overexpression of the Flii gene
- Author
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Thomsen, Nicole, primary, Chappell, Anna, additional, Ali, Radiya G., additional, Jones, Tamsin, additional, Adams, Damian H., additional, Matthaei, Klaus I., additional, Campbell, Hugh D., additional, Cowin, Allison J., additional, and Arkell, Ruth M., additional
- Published
- 2011
- Full Text
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18. Gender specific effects on the actin-remodelling protein Flightless I and TGF-β1 contribute to impaired wound healing in aged skin
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Adams, Damian H., primary, Strudwick, Xanthe L., additional, Kopecki, Zlatko, additional, Hooper-Jones, Jane A., additional, Matthaei, Klaus I., additional, Campbell, Hugh D., additional, Powell, Barry C., additional, and Cowin, Allison J., additional
- Published
- 2008
- Full Text
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19. Evaluation of a topical treatment for the relief of sensitive skin.
- Author
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Heinicke, Ingrid R., Adams, Damian H., Barnes, Tanya M., and Greive, Kerryn A.
- Subjects
SKIN inflammation ,OINTMENTS ,PHYSIOLOGICAL effects of collagen ,ERYTHEMA ,TRANSFORMING growth factors-beta ,THERAPEUTICS - Abstract
Background: Approximately, 50% of the population claim to have sensitive skin, which has created an important challenge for dermatologists and the cosmetic industry. This study evaluates the properties of QV Face Rescue Gel (Rescue Gel) that contains a combination of moisturizing and anti-irritant ingredients, and which is used to relieve the symptoms of sensitive facial skin. Methods: The ability of Rescue Gel to induce collagen types I and III in cultured neonatal human foreskin fibroblasts compared to transforming growth factor beta 1, a known potent inducer of collagen types I and III, was measured using immunofluorescence staining. Furthermore, healthy volunteers were recruited to measure the potential for Rescue Gel to reduce erythema induced by solar-simulated ultraviolet radiation on the skin compared to 0.5% hydrocortisone cream (positive control) as well as it's ability to decrease transepidermal water loss compared to baseline levels. In addition, the formulation was tested for its potential to be 1) nonstinging using a facial sting/discomfort assay performed on volunteers who reacted positively to lactic acid, 2) nonirritating as determined by repeat insult patch tests, and 3) noncomedogenic. Results: Rescue Gel significantly induced collagen types I and III in cultured human foreskin fibroblasts similarly to transforming growth factor beta 1. In volunteers, Rescue Gel was shown to significantly reduce erythema induced by solar-simulated ultraviolet radiation similarly to 0.5% hydrocortisone, and to significantly reduce transepidermal water loss compared to baseline levels. Further, the formulation was found to be nonstinging, nonirritating, and noncomedogenic. No adverse events were observed. Conclusion: In this study, Rescue Gel has been shown to exhibit properties that make it effective for use on sensitive or irritated facial skin, without exacerbation of the symptoms associated with sensitive skin. [ABSTRACT FROM AUTHOR]
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- 2015
- Full Text
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20. Differential Effects of Insulin-Like Growth Factors on Scratch Wound Repair in Respiratory Epithelial Cells
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Adams, Damian H., primary, McIntosh, David, additional, Wormald, Peter-John, additional, and Cowin, Allison J., additional
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- 2006
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21. Systemic delivery of anti-integrin aL antibodies reduces early macrophage recruitment, inflammation, and scar formation in murine burn wounds
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Michael Samuel, Damian H. Adams, Xanthe L. Strudwick, Allison J. Cowin, Natasha T. Pyne, Rachael Z. Murray, Strudwick, Xanthe L, Adams, Damian H, Pyne, Natasha T, Samuel, Michael S, Murray, Rachael Z, and Cowin, Allison J
- Subjects
0301 basic medicine ,Male ,integrin ,Integrin Alpha-L ,Inflammation ,macrophage ,Critical Care and Intensive Care Medicine ,Transforming Growth Factor beta1 ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Cicatrix ,Mice ,burns ,0302 clinical medicine ,Fibrosis ,medicine ,TNFα ,Macrophage ,Animals ,Humans ,CD11a Antigen ,Monocyte extravasation ,Discovery Express ,Mice, Inbred BALB C ,Wound Healing ,business.industry ,Tumor Necrosis Factor-alpha ,Macrophages ,TGFβ1 ,Antibodies, Monoclonal ,medicine.disease ,Extravasation ,030104 developmental biology ,Immunology ,Emergency Medicine ,Tumor necrosis factor alpha ,Collagen ,medicine.symptom ,Inflammation Mediators ,Wound healing ,business ,Burns - Abstract
Objective: Increased macrophage recruitment in the early stages of wound healing leads to an excessive inflammatory response associated with elevated fibrosis and scarring. This recruitment relies upon integrins on the surface of monocytes that regulate their migration and extravasation from the circulation into the wound site, where they differentiate into macrophages. The aim of this study was to determine if inhibiting monocyte extravasation from the circulation into burns would reduce macrophages numbers in burns and lead to reduced inflammation and scar formation. Approach: Scald burns were created on mice and treated with integrin alpha L (αL) function blocking antibody via intravenous delivery day 1 after injury. The effect of inhibiting macrophage recruitment into the burn was assessed using macro- and microscopic wound parameters as well as immunohistochemistry for inflammatory cell markers, cytokines, and collagen deposition. Results: Burn wound-associated macrophages were reduced by 54.7% at day 3 following treatment with integrin αL antibody, with levels returning to normal by day 7. This reduction in macrophages led to a concomitant reduction in inflammatory mediators, including tumor necrosis factor-alpha (TNFα) and Il-10 as well as a reduction in proscarring transforming growth factor beta 1 (TGFβ1). This reduced inflammatory response was also associated with less alpha smooth muscle actin (αSMA) expression and an overall trend toward reduced scar formation with a lower collagen I/III ratio. Innovation: Treatment of burns with integrin αL function blocking antibodies reduces inflammation in burn wounds. Conclusion: These results suggest that reducing macrophage infiltration into burn wounds may lead to a reduced early inflammatory response and less scar formation following burn injury. Refereed/Peer-reviewed
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- 2020
22. Native Australian plant extracts differentially induce Collagen I and Collagen III in vitro and could be important targets for the development of new wound healing therapies
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Qingyao Shou, Hans Wohlmuth, Damian H. Adams, Allison J. Cowin, Adams, Damian H, Shou, Qingyao, Wohlmuth, Hans, and Cowin, Allison J
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collagen ,0301 basic medicine ,Collagen i ,Myrtaceae ,Human skin ,Biology ,Pharmacology ,fibroblast ,Australian native plants ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Geijera parviflora ,0302 clinical medicine ,Coumarins ,Drug Discovery ,Botany ,medicine ,Humans ,Fibroblast ,Rutaceae ,Cells, Cultured ,Skin ,Wound Healing ,Molecular Structure ,integumentary system ,Plant Extracts ,Collagen iii ,Australia ,General Medicine ,Fibroblasts ,biology.organism_classification ,In vitro ,modulation ,030104 developmental biology ,medicine.anatomical_structure ,Rhodomyrtus psidioides ,fluorescence ,Collagen ,Wound healing ,Scrophulariaceae - Abstract
Australian native plants have a long history of therapeutic use in indigenous cultures, however, they have been poorly studied scientifically. We analysed the effects of 14 plant derived compounds from the species Pilidiostigma glabrum, Myoporum montanum, Geijera parviflora, and Rhodomyrtus psidioides for their potential wound healing properties by assessing their ability to induce or suppress Collagen I and Collagen III expression in human skin fibroblasts in culture. The compound 7-geranyloxycoumarin was able to significantly increase Collagen I (23.7%, p < 0.0002) expression in comparison to control. Significant suppression of Collagen III was observed for the compounds flindersine (11.1%, p < 0.02), and (N-acetoxymethyl) flindersine (27%, p < 0.00005). The implications of these finding is that these compounds could potentially alter the expression of different collagens in the skin allowing for the potential development of new wound healing therapies and new approaches for treating various skin diseases as well as photo (sun) damaged, and aged skin. Refereed/Peer-reviewed
- Published
- 2016
23. In vivo delivery of functional Flightless I siRNA using layer-by-layer polymer surface modification
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Kathryn R Penzkover, Xanthe L. Strudwick, Damian H. Adams, Allison J. Cowin, Laura A. Poole-Warren, Penny J. Martens, Mai Ly, Martens, Penny J, Ly, Mai, Adams, Damian H, Penzkover, Kathryn R, Strudwick, Xanthe, Cowin, Allison J, and Poole-Warren, Laura A
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Small interfering RNA ,Materials science ,Polymers ,Surface Properties ,Biomedical Engineering ,Biocompatible Materials ,Cell Line ,Green fluorescent protein ,Biomaterials ,Mice ,Tissue culture ,In vivo ,Animals ,Gene silencing ,RNA, Small Interfering ,chemistry.chemical_classification ,Mice, Inbred BALB C ,animal model ,Microfilament Proteins ,Polymer ,Molecular biology ,Cytoskeletal Proteins ,chemistry ,polyurethane ,siRNA ,drug delivery ,Drug delivery ,NIH 3T3 Cells ,Trans-Activators ,Biophysics ,Surface modification ,Carrier Proteins - Abstract
Gene silencing using small interfering RNA has been proposed as a therapy for cancer, viral infections and other diseases. This study aimed to investigate whether layer-by-layer polymer surface modification could deliver small interfering RNA to decrease fibrotic processes associated with medical device implantation. Anti-green fluorescent protein labelled small interfering RNA was applied to tissue culture plates and polyurethane using a layer-by-layer technique with small interfering RNA and poly-L-lysine. In vitro studies showed that the level of down-regulation of green fluorescent protein was directly related to the number of coatings applied. This layer-by-layer coating technique was then used to generate Rhodamine-Flii small interfering RNA-coated implants for in vivo studies of small interfering RNA delivery via subcutaneous implantation in mice. After two days, Rh-positive cells were observed on the implants surface indicating cellular uptake of the Rhodamine-Flii small interfering RNA. Decreased Flii gene expression was observed in tissue surrounding the Rhodamine-Flii small interfering RNA coated implants for up to seven days post implantation, returning to baseline by day 21. Genes downstream from Flii, including TGF-β1 and TGF-β3, showed significantly altered expression confirming a functional effect of the Rhodamine-Flii small interfering RNA on gene expression. This research demonstrates proof-of-principle that small interfering RNA can be delivered via layer-by-layer coatings on biomaterials and thereby can alter the fibrotic process. Refereed/Peer-reviewed
- Published
- 2015
24. Gender specific effects on the actin-remodelling protein Flightless I and TGF-β1 contribute to impaired wound healing in aged skin
- Author
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Jane A. Hooper-Jones, Allison J. Cowin, Klaus I. Matthaei, Hugh Campbell, Barry C. Powell, Damian H. Adams, Xanthe L. Strudwick, Zlatko Kopecki, Adams, Damian H, Strudwick, Xanthe L, Kopecki, Zlatko, Hooper-Jones, Jane A, Matthaei, Klaus I, Campbell, Hugh D, Powell, Barry C, and Cowin, Allison
- Subjects
Male ,Genetically modified mouse ,Aging ,Cytoplasm ,medicine.medical_specialty ,actin cytoskeleton ,wound ,Estrogen receptor ,Mice, Transgenic ,Biology ,Biochemistry ,Transforming Growth Factor beta1 ,Mice ,Sex Factors ,Downregulation and upregulation ,Internal medicine ,Gene expression ,medicine ,Animals ,Humans ,Secretion ,Cell Nucleus ,Wound Healing ,Estradiol ,integumentary system ,Epidermis (botany) ,Microfilament Proteins ,aging ,Cell Biology ,Fibroblasts ,Actin cytoskeleton ,Up-Regulation ,Cytoskeletal Proteins ,Protein Transport ,Endocrinology ,Immunology ,Trans-Activators ,Female ,Carrier Proteins ,Wound healing - Abstract
Impaired wound healing in the elderly presents a major clinical challenge. Understanding the cellular mechanisms behind age-related impaired healing is vital for developing new wound therapies. Here we show that the actin-remodelling protein, Flightless I (FliI) is a contributing factor to the poor healing observed in elderly skin and that gender plays a major role in this process. Using young and aged, wild-type and FliI overexpressing mice we found that aging significantly elevated FliI expression in the epidermis and wound matrix. Aging exacerbated the negative effect of FliI on wound repair and wounds in aged FliI transgenic mice were larger with delayed reepithelialisation. When the effect of gender was further analysed, despite increased FliI expression in young and aged male and female mice, female FliI transgenic mice had the most severe wound healing phenotype suggesting that male mice were refractory to FliI gene expression. Of potential importance, males, but not females, up-regulated transforming growth factor-β1 and this was most pronounced in aged male FliI overexpressing wounds. As FliI also functions as a co-activator of the estrogen nuclear receptor, increasing concentrations of β-estradiol were added to skin fibroblasts and keratinocytes and significantly enhanced FliI expression and translocation of FliI from the cytoplasm to the nucleus was observed. FliI further inhibited estrogen-mediated collagen I secretion suggesting a mechanism via which FliI may directly affect provisional matrix synthesis. In summary, FliI is a contributing factor to impaired healing and strategies aimed at decreasing FliI levels in elderly skin may improve wound repair. Refereed/Peer-reviewed
- Published
- 2008
25. Tropomyosin Regulates Cell Migration during Skin Wound Healing
- Author
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Cuc T. Bach, Yu Wooi Ching, Anthony J. Kee, Edna C. Hardeman, Geraldine M. O'Neill, Damian H. Adams, Justin G. Lees, Allison J. Cowin, Peter W. Gunning, Michael S. Samuel, Lees, Justin G, Ching, Yu Wooi, Adams, Damian H, Bach, Cuc TT, Samuel, Michael S, Kee, Anthony J, Hardeman, Edna C, Gunning, Peter, Cowin, Allison J, and O'Neill, Geraldine M
- Subjects
wound healing ,Tropomyosin ,Biochemistry ,Extracellular matrix ,tropomyosin ,Mice ,0302 clinical medicine ,Cell Movement ,animal ,Phosphorylation ,Mmce ,Cells, Cultured ,Skin ,Mice, Knockout ,0303 health sciences ,Cell migration ,cell line ,Cell biology ,Extracellular Matrix ,rac GTP-Binding Proteins ,Rac GTP-Binding Proteins ,animals ,GTP-binding proteins ,030220 oncology & carcinogenesis ,Models, Animal ,Female ,signal transduction ,Signal Transduction ,tumor ,skin ,extracellular matrix ,Actin filament organization ,Mice, Transgenic ,Dermatology ,macromolecular substances ,Biology ,03 medical and health sciences ,models ,Cell Line, Tumor ,Animals ,cultured ,Molecular Biology ,Actin ,030304 developmental biology ,Cell Proliferation ,Wound Healing ,Actin remodeling ,Cell Biology ,Mice, Inbred C57BL ,cell proliferation ,cells ,Paxillin ,Wound healing - Abstract
Precise orchestration of actin polymer into filaments with distinct characteristics of stability, bundling, and branching underpins cell migration. A key regulator of actin filament specialization is the tropomyosin family of actin-associating proteins. This multi-isoform family of proteins assemble into polymers that lie in the major groove of polymerized actin filaments, which in turn determine the association of molecules that control actin filament organization. This suggests that tropomyosins may be important regulators of actin function during physiological processes dependent on cell migration, such as wound healing. We have therefore analyzed the requirement for tropomyosin isoform expression in a mouse model of cutaneous wound healing. We find that mice in which the 9D exon from the TPM3/γTm tropomyosin gene is deleted (γ9D -/-) exhibit a more rapid wound-healing response 7 days after wounding compared with wild-type mice. Accelerated wound healing was not associated with increased cell proliferation, matrix remodeling, or epidermal abnormalities, but with increased cell migration. Rac GTPase activity and paxillin phosphorylation are elevated in cells from γ9D -/- mice, suggesting the activation of paxillin/Rac signaling. Collectively, our data reveal that tropomyosin isoform expression has an important role in temporal regulation of cell migration during wound healing. Refereed/Peer-reviewed
- Published
- 2015
26. Evaluation of a topical treatment for the relief of sensitive skin
- Author
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Ingrid R Heinicke, Damian H. Adams, Tanya M Barnes, Kerryn A Greive, Heinicke, Ingrid R, Adams, Damian H, Barnes, Tanya M, and Greive, Kerryn A
- Subjects
collagen ,Pathology ,medicine.medical_specialty ,Erythema ,Population ,Dermatology ,Pharmacology ,Sensitive skin ,Foreskin ,medicine ,education ,Adverse effect ,Hydrocortisone ,Original Research ,moisturization ,education.field_of_study ,Transepidermal water loss ,integumentary system ,business.industry ,Sting ,medicine.anatomical_structure ,Clinical, Cosmetic and Investigational Dermatology ,inflammation ,sensitive skin ,medicine.symptom ,business ,erythema ,medicine.drug - Abstract
Ingrid R Heinicke,1 Damian H Adams,2 Tanya M Barnes,1 Kerryn A Greive1 1Ego Pharmaceuticals, Braeside, VIC, Australia; 2Mawson Institute, University of South Australia, Mawson Lakes, SA, Australia Background: Approximately, 50% of the population claim to have sensitive skin, which has created an important challenge for dermatologists and the cosmetic industry. This study evaluates the properties of QV Face Rescue Gel (Rescue Gel) that contains a combination of moisturizing and anti-irritant ingredients, and which is used to relieve the symptoms of sensitive facial skin. Methods: The ability of Rescue Gel to induce collagen types I and III in cultured neonatal human foreskin fibroblasts compared to transforming growth factor beta 1, a known potent inducer of collagen types I and III, was measured using immunofluorescence staining. Furthermore, healthy volunteers were recruited to measure the potential for Rescue Gel to reduce erythema induced by solar-simulated ultraviolet radiation on the skin compared to 0.5% hydrocortisone cream (positive control) as well as it's ability to decrease transepidermal water loss compared to baseline levels. In addition, the formulation was tested for its potential to be 1) nonstinging using a facial sting/discomfort assay performed on volunteers who reacted positively to lactic acid, 2) nonirritating as determined by repeat insult patch tests, and 3) noncomedogenic. Results: Rescue Gel significantly induced collagen types I and III in cultured human foreskin fibroblasts similarly to transforming growth factor beta 1. In volunteers, Rescue Gel was shown to significantly reduce erythema induced by solar-simulated ultraviolet radiation similarly to 0.5% hydrocortisone, and to significantly reduce transepidermal water loss compared to baseline levels. Further, the formulation was found to be nonstinging, nonirritating, and noncomedogenic. No adverse events were observed. Conclusion: In this study, Rescue Gel has been shown to exhibit properties that make it effective for use on sensitive or irritated facial skin, without exacerbation of the symptoms associated with sensitive skin. Keywords: sensitive skin, inflammation, moisturization, collagen, erythema
- Published
- 2015
27. Conceptualising a Child-Centric Paradigm
- Author
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Adams, Damian H
- Subjects
child welfare ,reproductive techniques, assisted ,ethics ,policy - Abstract
Since its inception, donor conception practices have been a reproductive choice for the infertile. Past and current practices have the potential to cause significant and lifelong harm to the offspring through loss of kinship, heritage, identity, and family health history, and possibly through introducing physical problems. Legislation and regulation in Australia that specifies that the welfare of the child born as a consequence of donor conception is paramount may therefore be in conflict with the outcomes. Altering the paradigm to a child-centric model, however, impinges on reproductive choice and rights of adults involved in the process. With some lobby groups pushing for increased reproductive choice while others emphasise offspring rights there is a dichotomy of interests that society and legislators need to address. Concepts pertaining to a shift toward a child-centric paradigm are discussed. usc Refereed/Peer-reviewed
- Published
- 2013
28. Flii neutralizing antibodies improve wound healing in porcine preclinical studies
- Author
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Jackson, Jessica E, Kopecki, Zlatko, Adams, Damian H, and Cowin, Allison J
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nonhuman ,surgical wound ,integumentary system ,cell migration ,animal model ,animal experiment ,article ,swine ,wound healing ,wound dressing ,myofibroblast ,animal tissue ,histology ,cell proliferation ,female ,male ,priority journal ,tensile strength ,scar formation ,epithelization ,controlled study ,protein expression ,mouse - Abstract
Wound healing is an important area of widely unmet medical need, with millions of procedures carried out worldwide which could potentially benefit from a product to improve the wound repair process. Our studies investigating the actin-remodeling protein Flightless I (Flii) show it to be an important regulator of wound healing. Flii-deficient mice have enhanced wound healing in comparison to Flii overexpressing mice which have impaired wound healing. For the first time, we show that a Flightless I neutralizing monoclonal antibody (FnAb) therapy is effective in a large animal model of wound repair. Porcine 5 cm incisional and 6.25 cm2 excisional wounds were treated with FnAb at the time of wounding and for two subsequent days. The wounds were dressed in Tegaderm dressings and left to heal by secondary intention for 7 and 35 days, respectively. At the relevant end points, the wounds were excised and processed for histological analysis. Parameters of wound area, collagen deposition, and scar appearance were analyzed. The results show that treatment with FnAb accelerates reepithelialization and improves the macroscopic appearance of early scars. FnAbs have the potential to enhance wound repair and reduce scar formation. Refereed/Peer-reviewed
- Published
- 2012
29. Mouse strains for the ubiquitous or conditional overexpression of the Flii gene
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Radiya G. Ali, Allison J. Cowin, Tamsin E. M. Jones, Anna Chappell, Nicole Thomsen, Klaus I. Matthaei, Damian H. Adams, Hugh Campbell, Ruth M. Arkell, Thomsen, Nicole, Chappell, Anna, Ali, Radiya G, Jones, Tamsin, Adams, Damian H, Matthaei, Klaus I, Campbell, Hugh D, Cowin, Allison J, and Arkell, Ruth M
- Subjects
Male ,RNA, Untranslated ,Time Factors ,Genotype ,Blotting, Western ,Receptors, Cytoplasmic and Nuclear ,Mice, Transgenic ,wound healing ,Mice ,Endocrinology ,Species Specificity ,Genetics ,Animals ,Actin-binding protein ,Allele ,ROSA26 ,Gene ,Skin ,Mice, Knockout ,Mice, Inbred BALB C ,Wound Healing ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,Muscles ,Myocardium ,Microfilament Proteins ,Brain ,Proteins ,Cell Biology ,Null allele ,Embryonic stem cell ,Cell biology ,Cytoskeletal Proteins ,Trans-Activators ,biology.protein ,Female ,Carrier Proteins ,Wound healing ,Gelsolin ,Spleen ,Function (biology) - Abstract
The gelsolin related actin binding protein, Flii, is able to regulate wound healing; mice with decreased Flii expression show improved wound healing whereas mice with elevated Flii expression exhibit impaired wound healing. In both mice and humans Flii expression increases with age and amelioration of FLII activity represents a possible therapeutic strategy for improved wound healing in humans. Despite analysis of Flii function in a variety of organisms we know little of the molecular mechanisms underlying Flii action. Two new murine alleles of Flii have been produced to drive constitutive or tissue-specific expression of Flii. Each strain is able to rescue the embryonic lethality associated with a Flii null allele and to impair wound healing. These strains provide valuable resources for ongoing investigation of Flii function in a variety of biological processes. genesis 49:681–688, 2011. © 2011 Wiley-Liss, Inc.
- Published
- 2011
30. Data on keratin expression in human cells cultured with Australian native plant extracts
- Author
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Allison J. Cowin, Damian H. Adams, Hans Wohlmuth, Qingyao Shou, Adams, Damian H, Shou, Qingyao, Wohlmuth, Hans, and Cowin, Allison June
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Pathology ,medicine.medical_specialty ,Immunofluorescence ,lcsh:Computer applications to medicine. Medical informatics ,01 natural sciences ,Geijera parviflora ,Tissue culture ,Australian native plants ,Keratin ,medicine ,lcsh:Science (General) ,Data Article ,chemistry.chemical_classification ,Multidisciplinary ,biology ,medicine.diagnostic_test ,integumentary system ,010405 organic chemistry ,keratin expression ,human cells ,biology.organism_classification ,Keratin 1 ,In vitro ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Biochemistry ,chemistry ,Rhodomyrtus psidioides ,lcsh:R858-859.7 ,Wound healing ,lcsh:Q1-390 - Abstract
Australian native plants have a long history of therapeutic use in indigenous cultures particularly for the treatment of wounds. We analysed 14 plant derived compounds from the species Pilidiostigma glabrum, Myoporum montanum, Geijera parviflora, and Rhodomyrtus psidioides for keratin 1, 5, 10 and 14 supporting the research article "Native Australian plant extracts differentially induce Collagen I and Collagen III in vitro and could be important targets for the development of new wound healing therapies" [5]. An in situ immunofluorescence assay was used in a 96 well tissue culture plate format to measure keratin expression in immortalised human keratinocytes (HaCaTs) exposed Australian native plant compounds to NMR spectra for the plant extracts are included in this article as is quantitative fluorescent intensity data of keratin 1, 5, 10 and 14 expression. Refereed/Peer-reviewed
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31. Gender specific effects on the actin-remodelling protein Flightless I and TGF-beta1 contribute to impaired wound healing in aged skin.
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Adams DH, Strudwick XL, Kopecki Z, Hooper-Jones JA, Matthaei KI, Campbell HD, Powell BC, and Cowin AJ
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- Animals, Carrier Proteins, Cell Nucleus metabolism, Cytoplasm metabolism, Cytoskeletal Proteins biosynthesis, Estradiol pharmacology, Female, Fibroblasts metabolism, Humans, Male, Mice, Mice, Transgenic, Microfilament Proteins, Protein Transport, Sex Factors, Trans-Activators, Up-Regulation, Aging physiology, Cytoskeletal Proteins physiology, Transforming Growth Factor beta1 physiology, Wound Healing physiology
- Abstract
Impaired wound healing in the elderly presents a major clinical challenge. Understanding the cellular mechanisms behind age-related impaired healing is vital for developing new wound therapies. Here we show that the actin-remodelling protein, Flightless I (FliI) is a contributing factor to the poor healing observed in elderly skin and that gender plays a major role in this process. Using young and aged, wild-type and FliI overexpressing mice we found that aging significantly elevated FliI expression in the epidermis and wound matrix. Aging exacerbated the negative effect of FliI on wound repair and wounds in aged FliI transgenic mice were larger with delayed reepithelialisation. When the effect of gender was further analysed, despite increased FliI expression in young and aged male and female mice, female FliI transgenic mice had the most severe wound healing phenotype suggesting that male mice were refractory to FliI gene expression. Of potential importance, males, but not females, up-regulated transforming growth factor-beta1 and this was most pronounced in aged male FliI overexpressing wounds. As FliI also functions as a co-activator of the estrogen nuclear receptor, increasing concentrations of beta-estradiol were added to skin fibroblasts and keratinocytes and significantly enhanced FliI expression and translocation of FliI from the cytoplasm to the nucleus was observed. FliI further inhibited estrogen-mediated collagen I secretion suggesting a mechanism via which FliI may directly affect provisional matrix synthesis. In summary, FliI is a contributing factor to impaired healing and strategies aimed at decreasing FliI levels in elderly skin may improve wound repair.
- Published
- 2008
- Full Text
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