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The establishment and spread of invasive species are directly related to intersexual interactions as dispersal and reproductive success are related to distribution, effective population size, and population growth. Accordingly, populations established by r‐selected species are particularly difficult to suppress or eradicate. One such species, the red swamp crayfish (Procambarus clarkii) is established globally at considerable ecological and financial costs to natural and human communities. Here, we develop a single nucleotide polymorphism (SNP) loci panel for P. clarkii using restriction‐associated DNA‐sequencing data. We use the SNP panel to successfully genotype 1800 individuals at 930 SNPs in southeastern Michigan, USA. Genotypic data were used to reconstruct pedigrees, which enabled the characterization of P. clarkii's mating system and statistical tests for associations among environmental, demographic, and phenotypic predictors and adult reproductive success estimates. We identified juvenile cohorts using genotype‐based pedigrees, body size, and sampling timing, which elucidated the breeding phenology of multiple introduced populations. We report a high prevalence of multiple paternity in each surveyed waterbody, indicating polyandry in this species. We highlight the use of newly developed rapid genomic assessment tools for monitoring population reproductive responses, effective population sizes, and dispersal during ongoing control efforts. [ABSTRACT FROM AUTHOR]

The enzymes obtained from the alteration of amino acids residues at positions 219, 246, 248, 325, and 328 in Arabidopsis thaliana glyoxalase 2-1 (GLX2-1) were overexpressed, purified, and characterized to identify residues essential for GLX2 substrate activity. The results revealed that an active GLX2 enzyme requires both the presence of a properly positioned metal center and significant nonmetal, enzyme-substrate contacts, with the most important tyrosine 255.

The characterization of human glyoxalase II (Glx2) overexpressed in rich medium and in minimal medium containing zinc, iron, or cobalt, and the resulting Glx2 analogues is carried out to determine the nature of the metal center in the Glx2 enzyme. The results suggested that Glx2 contains a Fe(II)Zn(II) center in vivo but their catalytic activity is attributed to Zn(II) in the Zn1 site.