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5 results on '"Adult, Aged, Brain"'

1. Definition of miRNAs Expression Profile in Glioblastoma Samples: The Relevance of Non-Neoplastic Brain Reference

Author

Visani, Michela, De Biase, Dario, Marucci, Gianluca, Taccioli, Cristian, Baruzzi, Agostino, Pession, Annalisa, Baruzzi, A., Albani, F., Calbucci, F., D'alessandro, R., Michelucci, R., Brandes, A., Eusebieusebi, V., Ceruti, S., Fainardi, E., Tamarozzi, R., Emiliani, E., Cavallo, M., Franceschi, E., Tosoni, A., Fiorica, F., Valentini, A., Depenni, R., Mucciarini, C., Crisi, G., Sasso, E., Biasini, C., Cavanna, L., Guidetti, D., Marcello, N., Pisanello, A., Cremonini, A. M., Guiducci, G., De Pasqua, S., Testoni, S., Agati, R., Ambrosetto, G., Bacci, A., Baldin, E., Baldrati, A., Barbieri, E., Bartolini, S., Bellavista, E., Bisulli, F., Bonora, E., Bunkheila, F., Carelli, V., Crisci, M., Dall'occa, P., Ferro, S., Franceschi, C., Frezza, G., Grasso, V., Leonardi, M., Mostacci, B., Palandri, G., Pasini, E., Pastore Trossello, M., Poggi, R., Riguzzi, P., Rinaldi, R., Rizzi, S., Romeo, G., Spagnolli, F., Tinuper, P., Trocino, C., Cerasoli, S., Dall'agata, M., Faedi, M., Frattarelli, M., Gentili, G., Giovannini, A., Iorio, P., Pasquini, U., Galletti, G., Guidi, C., Neri, W., Patuelli, A., Strumia, S., Casmiro, M., Gamboni, A., Rasi, F., Cruciani, G., Cenni, P., Dazzi, C., Guidi, A. R., Zumaglini, F., Amadori, A., Pasini, G., Pasquinelli, M., Pasquini, E., Polselli, A., Ravasio, A., Viti, B., Sintini, M., Ariatti, A., Bertolini, F., Bigliardi, G., Carpeggiani, P., Cavalleri, F., Meletti, S., Nichelli, P., Pettorelli, E., Pinna, G., Zunarelli, E., Artioli, F., Bernardini, I., Costa, M., Greco, G., Guerzoni, R., Stucchi, C., Iaccarino, C., Ragazzi, M., Rizzi, R., Zuccoli, G., Api, P., Cartei, F., Fallica, E., Granieri, E., Latini, F., Lelli, G., Monetti, C., Saletti, A., Schivalocchi, R., Seraceni, S., Tola, M. R., Urbini, B., Giorgi, C., Montanari, E., Cerasti, D., Crafa, P., Dascola, I., Florindo, I., Giombelli, E., Mazza, S., Ramponi, V., Servadei, F., Silini, E. M., Torelli, P., Immovilli, P., Morelli, N., Vanzo, C., Nobile, C., M. Visani, D. de Biase, G. Marucci, C. Taccioli, A. Baruzzi, A. Pession, Perno Study Group, F. Albani, V. Eusebi, F. Bisulli, V. Carelli, M. Leonardi, B. Mostacci, P. Tinuper, the PERNO Study group [, E. Bonora, and ]

Subjects
Male, Genetics and Molecular Biology (all), Adult, Aged, Brain, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Glioblastoma, Humans, MicroRNAs, Middle Aged, Real-Time Polymerase Chain Reaction, Reference Values, Statistics, Nonparametric, Biochemistry, Genetics and Molecular Biology (all), Agricultural and Biological Sciences (all), Gene Expression, Bioinformatics, Biochemistry, Surgical oncology, Nucleic Acids, metabolism, Female, Gene Expression Profiling, Molecular Cell Biology, Basic Cancer Research, Gene expression, normal adjacent the tumor, Neurological Tumors, methods, Gene Expression Regulation, brain tumors, glioblastoma, miRNAs, Multidisciplinary, Cancer Risk Factors, Medicine (all), Statistics, non-neoplastic brain, Real-time polymerase chain reaction, Oncology, miRNAs, Medicine, DNA microarray, brain RNA commercial reference, Research Article, Adult, Aged, Brain, Neoplastic, genetics/physiology, Glioblastoma, metabolism, Humans, Male, MicroRNAs, metabolism, Middle Aged, Real-Time Polymerase Chain Reaction, Reference Values, Statistics, Nonparametric, Science, Brain tumor, Biology, NO, Molecular Genetics, epileptic tissue, Text mining, microRNA, medicine, miRNA, business.industry, Computational Biology, Cancers and Neoplasms, medicine.disease, Gene expression profiling, Gene Expression Regulation, Cancer research, RNA, brain tumors, metabolism, Middle Aged, Real-Time Polymerase Chain Reaction, Reference Values, Statistic, business, metabolism, Humans, Male, MicroRNA, Glioblastoma Multiforme
Abstract

Glioblastoma is the most aggressive brain tumor that may occur in adults. Regardless of the huge improvements in surgery and molecular therapy, the outcome of neoplasia remains poor. MicroRNAs are small molecules involved in several cellular processes, and their expression is altered in the vast majority of tumors. Several studies reported the expression of different miRNAs in glioblastoma, but one of the most critical point in understanding glioblastoma miRNAs profile is the comparison of these studies. In this paper, we focused our attention on the non-neoplastic references used for determining miRNAs expression. The aim of this study was to investigate if using three different non-neoplastic brain references (normal adjacent the tumor, commercial total RNA, and epileptic specimens) could provide discrepant results. The analysis of 19 miRNAs was performed using Real-Time PCR, starting from the set of samples described above and the expression values compared. Moreover, the three different normal RNAs were used to determine the miRNAs profile in 30 glioblastomas. The data showed that different non-neoplastic controls could lead to different results and emphasize the importance of comparing miRNAs profiles obtained using the same experimental condition.

Published
2013

2. Assessment of normal-appearing white and gray matter in patients with primary progressive multiple sclerosis - A diffusion-tensor magnetic resonance imaging study

Author

Vittorio Martinelli, Antonio Bertolotto, Roberto Bergamaschi, Domenico Caputo, Massimo Filippi, Giancarlo Comi, Giovanni Luigi Mancardi, Marco Bozzali, Enrico Montanari, Marco Rovaris, G. Iannucci, Angelo Ghezzi, Ruggero Capra, Rovaris, M, Bozzali, M, Iannucci, G, Ghezzi, A, Caputo, D, Montanari, E, Bertolotto, A, Bergamaschi, R, Capra, R, Mancardi, Gl, Martinelli, V, Comi, G, and Filippi, M

Subjects
Adult, Male, Image Processing, Primary Progressive Multiple Sclerosis, Scleroderma, Central nervous system disease, Lesion, Computer-Assisted, Arts and Humanities (miscellaneous), Fractional anisotropy, Image Processing, Computer-Assisted, Humans, Medicine, In patient, Pathological, Adult, Aged, Brain, pathology, Female, Humans, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Middle Aged, Scleroderma, Systemic, pathology, Aged, Scleroderma, Systemic, medicine.diagnostic_test, business.industry, Multiple sclerosis, Brain, Magnetic resonance imaging, Middle Aged, medicine.disease, Magnetic Resonance Imaging, Female, Neurology (clinical), medicine.symptom, business, Nuclear medicine
Abstract

Background: Diffusion-tensor magnetic resonance imaging is sensitive to the more destructive aspects of multiple sclerosis (MS) evolution occurring outside and within T2-visible lesions and, as a consequence, holds promise for providing a more complete picture of primary progressive (PP) MS–related tissue damage than conventional magnetic resonance imaging. Objective: To improve our understanding of PPMS by assessing the extent of occult pathological features in the normal-appearing white and gray matter of the brain using diffusion-tensor magnetic resonance imaging. Methods: Ninety-six patients with PPMS, 47 patients with secondary progressive (SP) MS, and 44 healthy control subjects were studied. T2-hyperintense and T1-hypointense lesion volumes were calculated, and the volume of the whole brain tissue was measured. Diffusiontensor magnetic resonance imaging scans were postprocessed and analyzed to obtain the mean diffusivity and fractional anisotropy histograms from the brain and from the normal-appearing white and gray matter in isolation. Results: The mean T2-hyperintense and T1-hypointense lesion volumes were lower in patients with PPMS than in patients with SPMS, while the mean absolute brain volumes were similar in the 2 groups. The average lesion diffusivity was significantly higher in patients with SPMS than in patients with PPMS (P.001). Histogram-derived metrics of the brain tissue and normal-appearing white and gray matter were significantly different between patients with PPMS and healthy subjects (range, P=.004 to.001). Average diffusivity values were significantly higher in patients with SPMS than in patients with PPMS for all the tissues studied (range, P=.001 to .001). Fractional anisotropy histogram–derived quantities did not significantly differ between the 2 patient groups (range, P=.94 to .03). Conclusion: This study confirms that, in patients with PPMS, normal-appearing white and gray matter are not spared by disease-related pathological processes, although they are affected to a lesser degree than in patients with SPMS. Arch Neurol. 2002;59:1406-1412

Published
2002

3. Diffusion MRI in patients with transient ischemic attacks

Author

Kidwell, C. S., Alger, J. R., Di Salle, F., Starkman, S., Villablanca, P., Bentson, J., and Jeffrey Saver

Subjects
Adult, Male, Time Factors, diagnosis, Ischemic Attack, Transient, Brain, Middle Aged, Magnetic Resonance Imaging, methods, Cerebrovascular Disorders, Adult, Aged, Brain, pathology, Cerebrovascular Disorders, diagnosis, Female, Humans, Ischemic Attack, diagnosis, Magnetic Resonance Imaging, methods, Male, Middle Aged, Time Factors, Humans, pathology, Female, Aged
Published
1999

4. [99mTc]-HM-PAO SPECT in Parkinson's disease

Author

C. Da Col, Pierluigi Zanco, B. Saitta, Leontino Battistin, N. Borsato, N. Perlotto, Mauro Dam, G Ferlin, Gilberto Pizzolato, Pizzolato, Gilberto, M., Dam, N., Borsato, B., Saitta, C. D., Col, N., Perlotto, P., Zanco, G., Ferlin, and L., Battistin

Subjects
Adult, Male, Pathology, medicine.medical_specialty, Parkinson's disease, pathology/physiopathology/radionuclide imaging, Posterior parietal cortex, Adult, Aged, Brain, pathology/physiopathology/radionuclide imaging, Cerebrovascular Circulation, Female, Humans, Male, Middle Aged, Organometallic Compounds, diagnostic use, Oximes, diagnostic use, Parkinson Disease, pathology, Technetium Tc 99m Exametazime, Technetium, diagnostic use, Tomography, Emission-Computed, pathology/physiopathology/radionuclide imaging, Cerebrovascular Circulation, Female, Humans, Male, Middle Aged, Organometallic Compound, Technetium Tc 99m Exametazime, Degenerative disease, diagnostic use, Oxime, Oximes, Basal ganglia, Organometallic Compounds, Humans, Medicine, Dementia, Tomography, Aged, business.industry, Brain, Technetium, Parkinson Disease, Middle Aged, medicine.disease, Neurology, Cerebral blood flow, Cerebrovascular Circulation, pathology, Female, Neurology (clinical), diagnostic use, Cardiology and Cardiovascular Medicine, business, Perfusion, Technetium-99m, Tomography, Emission-Computed
Abstract

Thirty-six patients affected by Parkinson's disease were studied using single photon emission computed tomography (SPECT) and [99mTc]–HM-PAO as a tracer. The scanning procedure was performed 16–24 h after discontinuation of specific therapy. Tracer activity ratios were determined in 10 pairs of cerebellar, cortical, and subcortical regions. Data were compared with those of 10 age-matched controls. Most of the regions examined did not show any relevant change between parkinsonian and control subjects. Notably, mean activity in striatal regions were similar in the two groups. Increased activity in caudate–putamen was found in patients who were on chronic DOPA therapy. Side-to-side asymmetries in the basal ganglia increased with the severity of the disease. Significant reductions of tracer uptake, from control values, were observed bilaterally in the parietal cortex. These deficits were more pronounced in patients with mental deterioration and in subjects who had been chronically treated with anticholinergic drugs. Parietal perfusion deficits in parkinsonian patients resemble those described in Alzheimer's dementia. These findings suggest that the heterogeneous alterations of regional cerebral blood flow (rCBF) in parkinsonian patients reflect the multifactorial pathophysiology of the disease.

Published
1988

5. In vivo assessment of the brain and cervical cord pathology of patients with primary progressive multiple sclerosis

Author

Giuseppe Santuccio, Marco Bozzali, Gianluigi Mancardi, Antonio Bertolotto, Massimo Filippi, Vittorio Martinelli, Roberto Bergamaschi, Giancarlo Comi, Enrico Montanari, Ruggero Capra, Marco Rovaris, Domenico Caputo, Angelo Ghezzi, Rovaris, M, Bozzali, M, Santuccio, G, Ghezzi, A, Caputo, D, Montanari, E, Bertolotto, A, Bergamaschi, R, Capra, R, Mancardi, G, Martinelli, V, Comi, Giancarlo, and Filippi, Massimo

Subjects
Adult, Male, Pathology, medicine.medical_specialty, Cord, Multiple Sclerosis, Central nervous system disease, Lesion, Atrophy, medicine, Humans, Aged, medicine.diagnostic_test, business.industry, Multiple sclerosis, Brain, Magnetic resonance imaging, Multiple Sclerosis, Chronic Progressive, Middle Aged, medicine.disease, Spinal cord, Magnetic Resonance Imaging, medicine.anatomical_structure, Chronic Progressive, Spinal Cord, Cervical Vertebrae, pathology, Female, Neurology (clinical), medicine.symptom, business, Adult, Aged, Brain, pathology, Cervical Vertebrae, Female, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Multiple Sclerosis, pathology, Spinal Cord, Cervical vertebrae
Abstract

In patients with primary progressive (PP) multiple sclerosis, brain MRI lesion activity and burden are low, despite the presence of severe neurological impairment. On the contrary, the degree of cord atrophy and diffuse tissue damage in the brain and cervical cord have been found to be associated with clinical disability. Against this background, this study aimed at providing an in vivo indirect assessment of brain and cervical cord pathology in a large cohort of PP multiple sclerosis patients, using conventional MRI and magnetization transfer imaging (MTI). Ninety-one PP multiple sclerosis patients, 36 secondary progressive (SP) multiple sclerosis patients and 30 healthy controls underwent brain and cervical cord MRI scans, using dual echo (brain) or fast short-tau inversion recovery (cervical cord) MTI and T(1)-weighted sequences. For the brain, T(2) hyperintense and T(1) hypointense lesion volumes were calculated and the volume of the whole of the brain tissue measured. For the cervical cord, the number and burden of lesions and the cross-sectional area were assessed. MTI scans were post-processed and analysed to obtain magnetization transfer ratio (MTR) histograms from the whole of the brain and cervical cord tissue and from the normal-appearing brain tissue in isolation. In PP multiple sclerosis patients, brain, normal-appearing brain tissue and cervical cord MTR histogram-derived metrics revealed the presence of diffuse tissue damage whose characteristics did not significantly differ from those of SP multiple sclerosis patients, even though SP multiple sclerosis patients had higher MRI-visible lesion burdens. None of the correlations between MRI or MTI measures obtained from the brain and the cord were significant. PP multiple sclerosis patients' disability was significantly, albeit weakly associated with a composite MR model including measures of loss and intrinsic damage of cervical cord tissue. Our data indicate the presence of a diffuse tissue damage undetectable by conventional MRI in PP multiple sclerosis patients, whose extent seems to match that of SP multiple sclerosis patients with similar levels of disability. They also suggest that the severity of multiple sclerosis pathology in the cervical cord is one of the factors contributing to neurological impairment in PP multiple sclerosis.

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