1. [Effect of plant polysaccharides on TH1-dependent immune response: screening investigation].
- Author
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Danilets MG, Bel'skiĭ IuP, Gur'ev AM, Belousov MV, Bel'skaia NV, Trofimova ES, Uchasova EG, Alhmedzhanov RR, Ligacheva AA, Iusbov MS, and Agefonov VI
- Subjects
- Animals, Cells, Cultured, Drug Evaluation, Preclinical, Erythrocytes immunology, Lipopolysaccharides pharmacology, Macrophages, Peritoneal enzymology, Mice, Mice, Inbred BALB C, Nitric Oxide biosynthesis, Nitric Oxide Synthase biosynthesis, Nitric Oxide Synthase immunology, Plant Extracts chemistry, Sheep, Th1 Cells metabolism, Macrophages, Peritoneal immunology, Nitric Oxide immunology, Plant Extracts pharmacology, Plants chemistry, Th1 Cells immunology
- Abstract
We have studied the influence of water-soluble polysaccharides isolated from Tussilago farfara L. leaves, Betula verrucosa Ehrh. leaves, Calendula officinalis L. flowers, Acorus calamus rhizomes, Inula helenium L. rhizomes, overground part of Trifolium pretense L., and overground part ofArtemisia absinthium L., on Thl immune response induced by sheep red blood cells and on NO production by murine peritoneal macrophages in vitro. All the investigated polysaccharides have stimulated a Th1 response. Polysaccharides isolated from Betula verrucosa leaves did not influence NO synthesis, while polysaccharides of Tussilago farfara leaves and Acorus calamus rhizomes stimulated NO synthase of murine macrophages on a level comparable with that of lipopolysaccharides (LPS). Polysaccharides from Inula helenium rhizomes, Calendula officinalis flowers, and overground parts of Trifolium pretense and Artemisia absinthium also stimulated NO production, but to a lower extent in comparison to LPS.
- Published
- 2010