1. The LBP/BPI multigenic family in invertebrates: Evolutionary history and evidences of specialization in mollusks
- Author
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Olga Baron, Emeline Deleury, Jean-Marc Reichhart, Christine Coustau, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Réponse immunitaire et developpement chez les insectes (RIDI - UPR 9002), Université de Strasbourg (UNISTRA)-Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Agence Nationale de la Recherche [ANR-07-BLAN-0214, ANR-12-EMMA-0007-01], European Research Council [AdG_20090506], CNRS, INRA, Universite de Strasbourg, and Universite Nice-Sophia Antipolis
- Subjects
0301 basic medicine ,Invertebrate immunity ,[SDV]Life Sciences [q-bio] ,Snails ,Immunology ,Snail ,Biomphalaria glabrata ,Genome ,Antimicrobial protein ,Conserved sequence ,03 medical and health sciences ,Immune system ,Species Specificity ,LBP/BPI ,Gene Duplication ,biology.animal ,Animals ,Conserved Sequence ,Invertebrate ,Genetics ,Membrane Glycoproteins ,030102 biochemistry & molecular biology ,biology ,Phylum ,Ecology ,Immunity ,Bacterial Infections ,Blood Proteins ,biology.organism_classification ,Biological Evolution ,Invertebrates ,humanities ,nervous system diseases ,Lipopolysaccharide binding ,030104 developmental biology ,Mycoses ,Organ Specificity ,[SDE]Environmental Sciences ,Carrier Proteins ,Transcriptome ,Acute-Phase Proteins ,Antimicrobial Cationic Peptides ,Developmental Biology - Abstract
International audience; LBPs (lipopolysaccharide binding proteins) and BPIs (bactericidal permeability increasing proteins) are important proteins involved in defense against bacterial pathogens. We recently discovered a novel biocidal activity of a LBP/BPI from the gastropod Biomphalaria glabrata and demonstrated its role in parental immune protection of eggs, highlighting the importance of LBP/BPIs in invertebrate immunity. Here we characterize four additional LBP/BPI from B. glabrata, presenting conserved sequence architecture and exon-intron structure. Searches of invertebrate genomes revealed that existence of LBP/BPIs is not a conserved feature since they are absent from phyla such as arthropods and platyhelminths. Analyses of LBP/BPI transcripts from selected mollusk species showed recent parallel duplications in some species, including B. glabrata. In this snail species, LBP/BPI members vary in their expression tissue localization as well as their change in expression levels after immune challenges (Gram-negative bacterium; Gram-positive bacterium or yeast). These results, together with the predicted protein features provide evidences of functional specialization of LBP/BPI family members in molluscs.
- Published
- 2016
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