1. Application of polypyrrole nanowires for the development of a tyrosinase biosensor
- Author
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Agnieszka Gilowska, Paweł Knihnicki, Jolanta Kochana, Katarzyna E. Hnida, Grzegorz D. Sulka, and Joanna Kozak
- Subjects
Conductive polymer ,Materials science ,General Chemical Engineering ,Tyrosinase ,Nanowire ,Analytical chemistry ,General Chemistry ,tyrosinase ,Polypyrrole ,polypyrrole nanowires ,biosensor ,Biochemistry ,Industrial and Manufacturing Engineering ,Matrix (chemical analysis) ,chemistry.chemical_compound ,chemistry ,Linear range ,Chemical engineering ,immobilization ,Materials Chemistry ,Glutaraldehyde ,Biosensor ,conductive polymers - Abstract
Polypyrrole nanowires (PPyNWs) were fabricated and examined as a structural component of amperometric biosensor matrix. An enzyme, tyrosinase (TYR), was immobilized onto PPyNWs using glutaraldehyde (GA). Matrix composite morphology was investigated using scanning electron microscopy. Electrochemical behavior of the prepared PPyNWs/GA/TYR biosensor towards catechol was studied and the assessment of its analytical characteristics was carried out taking into account linear range, sensitivity, repeatability, reproducibility and operational stability.
- Published
- 2015