45 results on '"Agua‐Doce, Ana"'
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2. Adaptive immunity and neutralizing antibodies against SARS-CoV-2 variants of concern following vaccination in patients with cancer: the CAPTURE study
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Fendler, Annika, Shepherd, Scott T. C., Au, Lewis, Wilkinson, Katalin A., Wu, Mary, Byrne, Fiona, Cerrone, Maddalena, Schmitt, Andreas M., Joharatnam-Hogan, Nalinie, Shum, Benjamin, Tippu, Zayd, Rzeniewicz, Karolina, Boos, Laura Amanda, Harvey, Ruth, Carlyle, Eleanor, Edmonds, Kim, Del Rosario, Lyra, Sarker, Sarah, Lingard, Karla, Mangwende, Mary, Holt, Lucy, Ahmod, Hamid, Korteweg, Justine, Foley, Tara, Bazin, Jessica, Gordon, William, Barber, Taja, Emslie-Henry, Andrea, Xie, Wenyi, Gerard, Camille L., Deng, Daqi, Wall, Emma C., Agua-Doce, Ana, Namjou, Sina, Caidan, Simon, Gavrielides, Mike, MacRae, James I., Kelly, Gavin, Peat, Kema, Kelly, Denise, Murra, Aida, Kelly, Kayleigh, O’Flaherty, Molly, Dowdie, Lauren, Ash, Natalie, Gronthoud, Firza, Shea, Robyn L., Gardner, Gail, Murray, Darren, Kinnaird, Fiona, Cui, Wanyuan, Pascual, Javier, Rodney, Simon, Mencel, Justin, Curtis, Olivia, Stephenson, Clemency, Robinson, Anna, Oza, Bhavna, Farag, Sheima, Leslie, Isla, Rogiers, Aljosja, Iyengar, Sunil, Ethell, Mark, Messiou, Christina, Cunningham, David, Chau, Ian, Starling, Naureen, Turner, Nicholas, Welsh, Liam, van As, Nicholas, Jones, Robin L., Droney, Joanne, Banerjee, Susana, Tatham, Kate C., O’Brien, Mary, Harrington, Kevin, Bhide, Shreerang, Okines, Alicia, Reid, Alison, Young, Kate, Furness, Andrew J. S., Pickering, Lisa, Swanton, Charles, Gandhi, Sonia, Gamblin, Steve, Bauer, David L. V., Kassiotis, George, Kumar, Sacheen, Yousaf, Nadia, Jhanji, Shaman, Nicholson, Emma, Howell, Michael, Walker, Susanna, Wilkinson, Robert J., Larkin, James, and Turajlic, Samra
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- 2021
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3. Functional antibody and T cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study
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Fendler, Annika, Au, Lewis, Shepherd, Scott T. C., Byrne, Fiona, Cerrone, Maddalena, Boos, Laura Amanda, Rzeniewicz, Karolina, Gordon, William, Shum, Benjamin, Gerard, Camille L., Ward, Barry, Xie, Wenyi, Schmitt, Andreas M., Joharatnam-Hogan, Nalinie, Cornish, Georgina H., Pule, Martin, Mekkaoui, Leila, Ng, Kevin W., Carlyle, Eleanor, Edmonds, Kim, Rosario, Lyra Del, Sarker, Sarah, Lingard, Karla, Mangwende, Mary, Holt, Lucy, Ahmod, Hamid, Stone, Richard, Gomes, Camila, Flynn, Helen R., Agua-Doce, Ana, Hobson, Philip, Caidan, Simon, Howell, Michael, Wu, Mary, Goldstone, Robert, Crawford, Margaret, Cubitt, Laura, Patel, Harshil, Gavrielides, Mike, Nye, Emma, Snijders, Ambrosius P., MacRae, James I., Nicod, Jerome, Gronthoud, Firza, Shea, Robyn L., Messiou, Christina, Cunningham, David, Chau, Ian, Starling, Naureen, Turner, Nicholas, Welsh, Liam, van As, Nicholas, Jones, Robin L., Droney, Joanne, Banerjee, Susana, Tatham, Kate C., Jhanji, Shaman, O’Brien, Mary, Curtis, Olivia, Harrington, Kevin, Bhide, Shreerang, Bazin, Jessica, Robinson, Anna, Stephenson, Clemency, Slattery, Tim, Khan, Yasir, Tippu, Zayd, Leslie, Isla, Gennatas, Spyridon, Okines, Alicia, Reid, Alison, Young, Kate, Furness, Andrew J. S., Pickering, Lisa, Gandhi, Sonia, Gamblin, Steve, Swanton, Charles, Nicholson, Emma, Kumar, Sacheen, Yousaf, Nadia, Wilkinson, Katalin A., Swerdlow, Anthony, Harvey, Ruth, Kassiotis, George, Larkin, James, Wilkinson, Robert J., and Turajlic, Samra
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- 2021
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4. Umbilical cord tissue–derived mesenchymal stromal cells maintain immunomodulatory and angiogenic potencies after cryopreservation and subsequent thawing
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Bárcia, Rita N., Santos, Jorge M., Teixeira, Mariana, Filipe, Mariana, Pereira, Ana Rita S., Ministro, Augusto, Água-Doce, Ana, Carvalheiro, Manuela, Gaspar, Maria Manuela, Miranda, Joana P., Graça, Luis, Simões, Sandra, Santos, Susana Constantino Rosa, Cruz, Pedro, and Cruz, Helder
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- 2017
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5. Adjuvant facilitates tolerance induction to factor VIII in hemophilic mice through a Foxp3-independent mechanism that relies on IL-10
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Oliveira, Vanessa G., Agua-Doce, Ana, Curotto de Lafaille, Maria A., Lafaille, Juan J., and Graca, Luis
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- 2013
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6. Different antibody-associated autoimmune diseases have distinct patterns of T follicular cell dysregulation
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Ribeiro, Filipa, Romão, Vasco C., Rosa, Sara, Jesus, Kátia, Agua-Doce, Ana, Barreira, Sofia, Martins, Patrícia, Silva, Susana, Nobre, Ema, Bugalho, Maria João, Fonseca, Valter R, Fonseca, João Eurico, Graca, Luis, and Repositório da Universidade de Lisboa
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Multidisciplinary ,Programmed Cell Death 1 Receptor ,Humans ,Lupus Erythematosus, Systemic ,T-Lymphocytes, Helper-Inducer ,Hashimoto Disease ,T-Lymphocytes, Regulatory ,Autoantibodies - Abstract
© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/., Autoantibodies are produced within germinal centers (GC), in a process regulated by interactions between B, T follicular helper (Tfh), and T follicular regulatory (Tfr) cells. The GC dysregulation in human autoimmunity has been inferred from circulating cells, albeit with conflicting results due to diverse experimental approaches. We applied a consistent approach to compare circulating Tfr and Tfh subsets in patients with different autoimmune diseases. We recruited 97 participants, including 72 patients with Hashimoto's thyroiditis (HT, n = 18), rheumatoid arthritis (RA, n = 16), or systemic lupus erythematosus (SLE, n = 32), and 31 matched healthy donors (HD). We found that the frequency of circulating T follicular subsets differed across diseases. Patients with HT had an increased frequency of blood Tfh cells (p = 0.0215) and a reduced Tfr/Tfh ratio (p = 0.0338) when compared with HD. This was not observed in patients with systemic autoimmune rheumatic diseases (RA, SLE), who had a reduction in both Tfh (p = 0.0494 and p = 0.0392, respectively) and Tfr (p = 0.0003 and p = 0.0001, respectively) cells, resulting in an unchanged Tfr/Tfh ratio. Activated PD-1+ICOS+Tfh and CD4+PD-1+CXCR5-Tph cells were raised only in patients with SLE (p = 0.0022 and p = 0.0054), without association with disease activity. Our data suggest that GC dysregulation, assessed by T follicular subsets, is not uniform in human autoimmunity. Specific patterns of dysregulation may become potential biomarkers for disease and patient stratification., This work was supported by the Fundação para a Ciência e Tecnologia, Portugal (EJPRD/0003/2019).
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- 2022
7. T follicular regulatory cells in mice and men
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Maceiras, Ana Raquel, Fonseca, Valter R., Agua‐Doce, Ana, and Graca, Luis
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- 2017
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8. Adaptive immunity and neutralizing antibodies against SARS-CoV-2 variants of concern following vaccination in patients with cancer: the CAPTURE study
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Fendler, Annika, Shepherd, Scott TC, Au, Lewis, Wilkinson, Katalin A, Wu, Mary, Byrne, Fiona, Cerrone, Maddalena, Schmitt, Andreas M, Joharatnam-Hogan, Nalinie, Shum, Benjamin, Tippu, Zayd, Rzeniewicz, Karolina, Boos, Laura Amanda, Harvey, Ruth, Carlyle, Eleanor, Edmonds, Kim, Del Rosario, Lyra, Sarker, Sarah, Lingard, Karla, Mangwende, Mary, Holt, Lucy, Ahmod, Hamid, Korteweg, Justine, Foley, Tara, Bazin, Jessica, Gordon, William, Barber, Taja, Emslie-Henry, Andrea, Xie, Wenyi, Gerard, Camille L, Deng, Daqi, Wall, Emma C, Agua-Doce, Ana, Namjou, Sina, Caidan, Simon, Gavrielides, Mike, MacRae, James I, Kelly, Gavin, Peat, Kema, Kelly, Denise, Murra, Aida, Kelly, Kayleigh, O���Flaherty, Molly, Dowdie, Lauren, Ash, Natalie, Gronthoud, Firza, Shea, Robyn L, Gardner, Gail, Murray, Darren, Kinnaird, Fiona, Cui, Wanyuan, Pascual, Javier, Rodney, Simon, Mencel, Justin, Curtis, Olivia, Stephenson, Clemency, Robinson, Anna, Oza, Bhavna, Farag, Sheima, Leslie, Isla, Rogiers, Aljosja, Iyengar, Sunil, Ethell, Mark, Messiou, Christina, Cunningham, David, Chau, Ian, Starling, Naureen, Turner, Nicholas, Welsh, Liam, van As, Nicholas, Jones, Robin L, Droney, Joanne, Banerjee, Susana, Tatham, Kate C, O���Brien, Mary, Harrington, Kevin, Bhide, Shreerang, Okines, Alicia, Reid, Alison, Young, Kate, Furness, Andrew JS, Pickering, Lisa, Swanton, Charles, Consortium, The Crick COVID-19, Gandhi, Sonia, Gamblin, Steve, Bauer, David LV, Kassiotis, George, Kumar, Sacheen, Yousaf, Nadia, Jhanji, Shaman, Nicholson, Emma, Howell, Michael, Walker, Susanna, Wilkinson, Robert J, Larkin, James, Turajlic, Samra, and Consortium, The CAPTURE
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Model organisms ,Chemical Biology & High Throughput ,Human Biology & Physiology ,FOS: Clinical medicine ,Stem Cells ,Genome Integrity & Repair ,Immunology ,Neurosciences ,Infectious Disease ,Cell Biology ,Tumour Biology ,Biochemistry & Proteomics ,Signalling & Oncogenes ,Metabolism ,Ecology,Evolution & Ethology ,Cell Cycle & Chromosomes ,Genetics & Genomics ,Developmental Biology ,Structural Biology & Biophysics ,Computational & Systems Biology - Abstract
Coronavirus disease 2019 (COVID-19) antiviral response in a pan-tumor immune monitoring (CAPTURE) (NCT03226886) is a prospective cohort study of COVID-19 immunity in patients with cancer. Here we evaluated 585 patients following administration of two doses of BNT162b2 or AZD1222 vaccines, administered 12 weeks apart. Seroconversion rates after two doses were 85% and 59% in patients with solid and hematological malignancies, respectively. A lower proportion of patients had detectable titers of neutralizing antibodies (NAbT) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOC) versus wild-type (WT) SARS-CoV-2. Patients with hematological malignancies were more likely to have undetectable NAbT and had lower median NAbT than those with solid cancers against both SARS-CoV-2 WT and VOC. By comparison with individuals without cancer, patients with hematological, but not solid, malignancies had reduced neutralizing antibody (NAb) responses. Seroconversion showed poor concordance with NAbT against VOC. Previous SARS-CoV-2 infection boosted the NAb response including against VOC, and anti-CD20 treatment was associated with undetectable NAbT. Vaccine-induced T cell responses were detected in 80% of patients and were comparable between vaccines or cancer types. Our results have implications for the management of patients with cancer during the ongoing COVID-19 pandemic.
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- 2022
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9. Functional antibody and T cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study
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Fendler, Annika, Au, Lewis, Shepherd, Scott TC, Byrne, Fiona, Cerrone, Maddalena, Boos, Laura Amanda, Rzeniewicz, Karolina, Gordon, William, Shum, Benjamin, Gerard, Camille L, Ward, Barry, Xie, Wenyi, Schmitt, Andreas M, Joharatnam-Hogan, Nalinie, Cornish, Georgina H, Pule, Martin, Mekkaoui, Leila, Ng, Kevin W, Carlyle, Eleanor, Edmonds, Kim, Del Rosario, Lyra, Sarker, Sarah, Lingard, Karla, Mangwende, Mary, Holt, Lucy, Ahmod, Hamid, Stone, Richard, Gomes, Camila, Flynn, Helen R, Agua-Doce, Ana, Hobson, Philip, Caidan, Simon, Howell, Michael, Wu, Mary, Goldstone, Robert, Crawford, Margaret, Cubitt, Laura, Patel, Harshil, Gavrielides, Mike, Nye, Emma, Snijders, Ambrosius P, MacRae, James I, Nicod, Jerome, Gronthoud, Firza, Shea, Robyn L, Messiou, Christina, Cunningham, David, Chau, Ian, Starling, Naureen, Turner, Nicholas, Welsh, Liam, van As, Nicholas, Jones, Robin L, Droney, Joanne, Banerjee, Susana, Tatham, Kate C, Jhanji, Shaman, O���Brien, Mary, Curtis, Olivia, Harrington, Kevin, Bhide, Shreerang, Bazin, Jessica, Robinson, Anna, Stephenson, Clemency, Slattery, Tim, Khan, Yasir, Tippu, Zayd, Leslie, Isla, Gennatas, Spyridon, Okines, Alicia, Reid, Alison, Young, Kate, Furness, Andrew JS, Pickering, Lisa, Gandhi, Sonia, Gamblin, Steve, Swanton, Charles, Consortium, The Crick COVID-19, Nicholson, Emma, Kumar, Sacheen, Yousaf, Nadia, Wilkinson, Katalin A, Swerdlow, Anthony, Harvey, Ruth, Kassiotis, George, Larkin, James, Wilkinson, Robert J, Turajlic, Samra, and consortium, The CAPTURE
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Model organisms ,Chemical Biology & High Throughput ,Human Biology & Physiology ,FOS: Clinical medicine ,Stem Cells ,Genome Integrity & Repair ,Immunology ,Neurosciences ,Infectious Disease ,Cell Biology ,Tumour Biology ,Biochemistry & Proteomics ,Signalling & Oncogenes ,Metabolism ,Ecology,Evolution & Ethology ,Cell Cycle & Chromosomes ,Genetics & Genomics ,Developmental Biology ,Structural Biology & Biophysics ,Computational & Systems Biology - Abstract
Patients with cancer have higher COVID-19 morbidity and mortality. Here we present the prospective CAPTURE study, integrating longitudinal immune profiling with clinical annotation. Of 357 patients with cancer, 118 were SARS-CoV-2 positive, 94 were symptomatic and 2 died of COVID-19. In this cohort, 83% patients had S1-reactive antibodies and 82% had neutralizing antibodies against wild type SARS-CoV-2, whereas neutralizing antibody titers against the Alpha, Beta and Delta variants were substantially reduced. S1-reactive antibody levels decreased in 13% of patients, whereas neutralizing antibody titers remained stable for up to 329 days. Patients also had detectable SARS-CoV-2-specific T cells and CD4+ responses correlating with S1-reactive antibody levels, although patients with hematological malignancies had impaired immune responses that were disease and treatment specific, but presented compensatory cellular responses, further supported by clinical recovery in all but one patient. Overall, these findings advance the understanding of the nature and duration of the immune response to SARS-CoV-2 in patients with cancer.
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- 2022
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10. Adaptive immunity and neutralizing antibodies against SARS-CoV-2 variants of concern following vaccination in patients with cancer: The CAPTURE study
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Fendler, Annika, primary, Shepherd, Scott, additional, Au, Lewis, additional, Wilkinson, Katalin, additional, Wu, Mary, additional, Byrne, Fiona, additional, Cerrone, Maddalena, additional, Schmitt, Andreas, additional, Joharatnam-Hogan, Nalinie, additional, Shum, Ben, additional, Tippu, Zayd, additional, Rzeniewicz, Karolina, additional, Boos, Laura, additional, Harvey, Ruth, additional, Carlyle, Eleanor, additional, Edmonds, Kim, additional, Rosario, Lyra Del, additional, Sarker, Sarah, additional, Lingard, Karla, additional, Mangwende, Mary, additional, Holt, Lucy, additional, Ahmod, Hamid, additional, Koreweg, Justine, additional, Foley, Tara, additional, Bazin, Jessica, additional, Gordon, William, additional, Barber, Taja, additional, Emslie-Henry, Andrea, additional, Xie, Wenyi, additional, Gerard, Camille, additional, Deng, Daqi, additional, Wall, Emma, additional, Agua-Doce, Ana, additional, Namjou, Sina, additional, Caidan, Simon, additional, Gavrielides, Mike, additional, MacRae, James, additional, Kelly, Gavin, additional, Peat, Kema, additional, Kelly, Denise, additional, Murra, Aida, additional, Kelly, Kayleigh, additional, O'Flaherty, Molly, additional, Dowdie, Lauren, additional, Ash, Natalie, additional, Grounthoud, Firza, additional, Shea, Robyn, additional, Gardner, Gail, additional, Murray, Darren, additional, Kinnaird, Fiona, additional, Cui, Wanyuan, additional, Pascual, Javier, additional, Rodney, Simon, additional, Mencel, Justin, additional, Curtis, Olivia, additional, Stephenson, Clemency, additional, Robinson, Anna, additional, Oza, Bhavna, additional, Farag, Sheima, additional, Leslie, Isla, additional, Rogiers, Aljosja, additional, Lyengar, Sunil, additional, Ethell, Mark, additional, Messiou, Christina, additional, Cunningham, David, additional, Chau, Ian, additional, Starling, Naureen, additional, Turner, Nicholas, additional, Welsh, Liam, additional, As, Nicholas van, additional, Jones, Robin, additional, DRoney, Joanne, additional, Banerjee, Susana, additional, Tatham, Kate, additional, O'Brien, Mary, additional, Harrington, Kevin, additional, Bhide, Shreerang, additional, Okines, Alicia, additional, Reid, Alison, additional, Young, Kate, additional, Furness, Andrew, additional, Pickering, Lisa, additional, Swanton, Charles, additional, Gandhi, Sonia, additional, Gamblin, Steve, additional, Bauer, David, additional, Kassiotis, George, additional, Kumar, Sacheen, additional, Yousaf, Nadia, additional, Jhanji, Shaman, additional, Nicholson, Emma, additional, Howell, Michael, additional, Walker, Susanna, additional, Wilkinson, Robert, additional, Larkin, James, additional, and Turajlic, Samra, additional
- Published
- 2021
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11. Functional antibody and T-cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study
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Fendler, Annika, primary, Au, Lewis, additional, Shepherd, Scott, additional, Byrne, Fiona, additional, Cerrone, Maddalena, additional, Boos, Laura, additional, Rzeniewicz, Karolina, additional, Gordon, William, additional, Shum, Ben, additional, Gerard, Camille, additional, Ward, Barry, additional, Xie, Wenyi, additional, Schmitt, Andreas, additional, Joharatnam-Hogan, Nalinie, additional, Cornish, Georgina, additional, Pule, Martin, additional, Mekkaoui, Leila, additional, Ng, Kevin, additional, Carlyle, Eleanor, additional, Edmonds, Kim, additional, Rosario, Lyra Del, additional, Sarker, Sarah, additional, Lingard, Karla, additional, Mangwende, Mary, additional, Holt, Lucy, additional, Ahmod, Hamid, additional, Stone, Richard, additional, Gomes, Camila, additional, Flynn, Helen, additional, Agua-Doce, Ana, additional, Hobson, Philip, additional, Caidan, Simon, additional, Howell, Michael, additional, Wu, Mary, additional, Goldstone, Robert, additional, Crawford, Margaret, additional, Cubitt, Laura, additional, Patel, Harshil, additional, Gavrielides, Mike, additional, Nye, Emma, additional, Snijders, Ambrosius, additional, MacRae, James, additional, Nicod, Jerome, additional, Gronthoud, Firza, additional, Shea, Robyn, additional, Messiou, Christina, additional, Cunningham, David, additional, Chau, Ian, additional, Starling, Naureen, additional, Turner, Nicholas, additional, Welsh, Liam, additional, As, Nicholas van, additional, Jones, Robin, additional, Droney, Joanne, additional, Banerjee, Susana, additional, Tatham, Kate, additional, Jhanji, Shaman, additional, O'Brien, Mary, additional, Curtis, Oliva, additional, Harrington, Kevin, additional, Bhide, Shreerang, additional, Bazin, Jessica, additional, Robinson, Anna, additional, Stephenson, Clemency, additional, Slattery, Tim, additional, Khan, Yasir, additional, Tippu, Zayd, additional, Leslie, Isla, additional, Gennatas, Spyridon, additional, Okines, Alicia, additional, Reid, Alison, additional, Young, Kate, additional, Furness, Andrew, additional, Pickering, Lisa, additional, Gandhi, Sonia, additional, Gamblin, Steve, additional, Swanton, Charles, additional, Nicholson, Emma, additional, Kumar, Sacheen, additional, Yousaf, Nadia, additional, Wilkinson, Katalin, additional, Swerdlow, Anthony, additional, Harvey, Ruth, additional, Kassiotis, George, additional, Larkin, James, additional, Wilkinson, Robert, additional, and Turajlic, Samra, additional
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- 2021
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12. The fate of CD4+ T cells under tolerance-inducing stimulation: a modeling perspective
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Caridade, Marta, Oliveira, Vanessa G, Agua-Doce, Ana, Graca, Luis, and Ribeiro, Ruy M
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- 2013
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13. Regulatory T cell maintenance of dominant tolerance: Induction of tissue self-defense?
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Oliveira, Vanessa, Água-Doce, Ana, Duarte, Joana, Soares, Miguel P., and Graca, Luis
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- 2006
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14. Adaptive immunity to SARS-CoV-2 in cancer patients: The CAPTURE study
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Fendler, Annika, primary, Au, Lewis, additional, Boos, Laura Amanda, additional, Byrne, Fiona, additional, Shepherd, Scott T.C., additional, Shum, Ben, additional, Gerard, Camille L., additional, Ward, Barry, additional, Xie, Wenyi, additional, Cerrone, Maddalena, additional, Cornish, Georgina H., additional, Pule, Martin, additional, Mekkaoui, Leila, additional, Ng, Kevin W., additional, Stone, Richard, additional, Gomes, Camila, additional, Flynn, Helen R., additional, Agua-Doce, Ana, additional, Hobson, Phillip, additional, Caidan, Simon, additional, Howell, Mike, additional, Goldstone, Robert, additional, Gavrielides, Mike, additional, Nye, Emma, additional, Snijders, Bram, additional, Macrae, James, additional, Nicod, Jerome, additional, Hayday, Adrian, additional, Gronthoud, Firza, additional, Messiou, Christina, additional, Cunningham, David, additional, Chau, Ian, additional, Starling, Naureen, additional, Turner, Nicholas, additional, Rusby, Jennifer, additional, Welsh, Liam, additional, van As, Nicholas, additional, Jones, Robin, additional, Droney, Joanne, additional, Banerjee, Susana, additional, Tatham, Kate, additional, Jhanji, Shaman, additional, O’Brien, Mary, additional, Curtis, Olivia, additional, Harrington, Kevin, additional, Bhide, Shreerang, additional, Slattery, Tim, additional, Khan, Yasir, additional, Tippu, Zayd, additional, Leslie, Isla, additional, Gennatas, Spyridon, additional, Okines, Alicia, additional, Reid, Alison, additional, Young, Kate, additional, Furness, Andrew, additional, Pickering, Lisa, additional, Ghandi, Sonia, additional, Gamblin, Steve, additional, Swanton, Charles, additional, Nicholson, Emma, additional, Kumar, Sacheen, additional, Yousaf, Nadia, additional, Wilkinson, Katalin, additional, Swerdlow, Anthony, additional, Harvey, Ruth, additional, Kassiotis, George, additional, Wilkinson, Robert, additional, Larkin, James, additional, and Turajlic, Samra, additional
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- 2020
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15. Preexisting and de novo humoral immunity to SARS-CoV-2 in humans
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Ng, Kevin W., primary, Faulkner, Nikhil, additional, Cornish, Georgina H., additional, Rosa, Annachiara, additional, Harvey, Ruth, additional, Hussain, Saira, additional, Ulferts, Rachel, additional, Earl, Christopher, additional, Wrobel, Antoni G., additional, Benton, Donald J., additional, Roustan, Chloe, additional, Bolland, William, additional, Thompson, Rachael, additional, Agua-Doce, Ana, additional, Hobson, Philip, additional, Heaney, Judith, additional, Rickman, Hannah, additional, Paraskevopoulou, Stavroula, additional, Houlihan, Catherine F., additional, Thomson, Kirsty, additional, Sanchez, Emilie, additional, Shin, Gee Yen, additional, Spyer, Moira J., additional, Joshi, Dhira, additional, O’Reilly, Nicola, additional, Walker, Philip A., additional, Kjaer, Svend, additional, Riddell, Andrew, additional, Moore, Catherine, additional, Jebson, Bethany R., additional, Wilkinson, Meredyth, additional, Marshall, Lucy R., additional, Rosser, Elizabeth C., additional, Radziszewska, Anna, additional, Peckham, Hannah, additional, Ciurtin, Coziana, additional, Wedderburn, Lucy R., additional, Beale, Rupert, additional, Swanton, Charles, additional, Gandhi, Sonia, additional, Stockinger, Brigitta, additional, McCauley, John, additional, Gamblin, Steve J., additional, McCoy, Laura E., additional, Cherepanov, Peter, additional, Nastouli, Eleni, additional, and Kassiotis, George, additional
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- 2020
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16. Adapting to the Coronavirus Pandemic: Building and Incorporating a Diagnostic Pipeline in a Shared Resource Laboratory
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Russell, Emma, primary, Agua‐Doce, Ana, additional, Carr, Lotte, additional, Malla, Asha, additional, Bartolovic, Kerol, additional, Levi, Dina, additional, Henderson, Carl, additional, Das, Debipriya, additional, Rhys, Hefin, additional, Hobson, Philip, additional, Purewal, Sukhveer, additional, and Riddell, Andrew, additional
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- 2020
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17. Pandemic peak SARS-CoV-2 infection and seroconversion rates in London frontline health-care workers
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Houlihan, Catherine F, primary, Vora, Nina, additional, Byrne, Thomas, additional, Lewer, Dan, additional, Kelly, Gavin, additional, Heaney, Judith, additional, Gandhi, Sonia, additional, Spyer, Moira J, additional, Beale, Rupert, additional, Cherepanov, Peter, additional, Moore, David, additional, Gilson, Richard, additional, Gamblin, Steve, additional, Kassiotis, George, additional, McCoy, Laura E, additional, Swanton, Charles, additional, Hayward, Andrew, additional, Nastouli, Eleni, additional, Aitken, Jim, additional, Allen, Zoe, additional, Ambler, Rachel, additional, Ambrose, Karen, additional, Ashton, Emma, additional, Avola, Alida, additional, Balakrishnan, Samutheswari, additional, Barns-Jenkins, Caitlin, additional, Barr, Genevieve, additional, Barrell, Sam, additional, Basu, Souradeep, additional, Beesley, Clare, additional, Bhardwaj, Nisha, additional, Bibi, Shahnaz, additional, Bineva-Todd, Ganka, additional, Biswas, Dhruva, additional, Blackman, Michael J, additional, Bonnet, Dominique, additional, Bowker, Faye, additional, Broncel, Malgorzata, additional, Brooks, Claire, additional, Buck, Michael D, additional, Buckton, Andrew, additional, Budd, Timothy, additional, Burrell, Alana, additional, Busby, Louise, additional, Bussi, Claudio, additional, Butterworth, Simon, additional, Byrne, Fiona, additional, Byrne, Richard, additional, Caidan, Simon, additional, Campbell, Joanna, additional, Canton, Johnathan, additional, Cardoso, Ana, additional, Carter, Nick, additional, Carvalho, Luiz, additional, Carzaniga, Raffaella, additional, Chandler, Natalie, additional, Chen, Qu, additional, Churchward, Laura, additional, Clark, Graham, additional, Clayton, Bobbi, additional, Cobolli Gigli, Clementina, additional, Collins, Zena, additional, Cottrell, Sally, additional, Crawford, Margaret, additional, Cubitt, Laura, additional, Cullup, Tom, additional, Davies, Heledd, additional, Davis, Patrick, additional, Davison, Dara, additional, D'Avola, Annalisa, additional, Dearing, Vicky, additional, Debaisieux, Solene, additional, Diaz-Romero, Monica, additional, Dibbs, Alison, additional, Diring, Jessica, additional, Driscoll, Paul C, additional, Earl, Christopher, additional, Edwards, Amelia, additional, Ekin, Chris, additional, Evangelopoulos, Dimitrios, additional, Faraway, Rupert, additional, Fearns, Antony, additional, Ferron, Aaron, additional, Fidanis, Efthymios, additional, Fitz, Dan, additional, Fleming, James, additional, Frederico, Bruno, additional, Gaiba, Alessandra, additional, Gait, Anthony, additional, Gaul, Liam, additional, Golding, Helen M, additional, Goldman, Jacki, additional, Goldstone, Robert, additional, Gomez Dominguez, Belen, additional, Gong, Hui, additional, Grant, Paul R, additional, Greco, Maria, additional, Grobler, Mariana, additional, Guedan, Anabel, additional, Gutierrez, Maximiliano G, additional, Hackett, Fiona, additional, Hall, Ross, additional, Halldorsson, Steinar, additional, Harris, Suzanne, additional, Hashim, Sugera, additional, Healy, Lyn, additional, Herbst, Susanne, additional, Hewitt, Graeme, additional, Higgins, Theresa, additional, Hindmarsh, Steve, additional, Hirani, Rajnika, additional, Hope, Joshua, additional, Horton, Elizabeth, additional, Hoskins, Beth, additional, Houlihan, Catherine F, additional, Howell, Michael, additional, Howitt, Louise, additional, Hoyle, Jacqueline, additional, Htun, Mint R, additional, Hubank, Michael, additional, Huerga Encabo, Hector, additional, Hughes, Deborah, additional, Hughes, Jane, additional, Huseynova, Almaz, additional, Hwang, Ming-Shih, additional, Instrell, Rachael, additional, Jackson, Deborah, additional, Jamal-Hanjani, Mariam, additional, Jenkins, Lucy, additional, Jiang, Ming, additional, Johnson, Mark, additional, Jones, Leigh, additional, Kanu, Nnennaya, additional, Kiely, Louise, additional, King Spert Teixeira, Anastacio, additional, Kirk, Stuart, additional, Kjaer, Svend, additional, Knuepfer, Ellen, additional, Komarov, Nikita, additional, Kotzampaltiris, Paul, additional, Kousis, Konstantinos, additional, Krylova, Tammy, additional, Kucharska, Ania, additional, Labrum, Robyn, additional, Lambe, Catherine, additional, Lappin, Michelle, additional, Lee, Stacey-Ann, additional, Levett, Andrew, additional, Levett, Lisa, additional, Levi, Marcel, additional, Liu, Hon-Wing, additional, Loughlin, Sam, additional, Lu, Wei-Ting, additional, MacRae, James I, additional, Madoo, Akshay, additional, Marczak, Julie A, additional, Martensson, Mimmi, additional, Martinez, Thomas, additional, Marzook, Bishara, additional, Matthews, John, additional, Matz, Joachim M, additional, McCall, Samuel, additional, McKay, Fiona, additional, McNamara, Edel C, additional, Minutti, Carlos M, additional, Mistry, Gita, additional, Molina-Arcas, Miriam, additional, Montaner, Beatriz, additional, Montgomery, Kylie, additional, Moore, Catherine, additional, Moraiti, Anastasia, additional, Moreira-Teixeira, Lucia, additional, Mukherjee, Joyita, additional, Naceur-Lombardelli, Cristina, additional, Nelson, Aileen, additional, Nicod, Jerome, additional, Nightingale, Luke, additional, Nofal, Stephanie, additional, Nurse, Paul, additional, Nutan, Savita, additional, Oedekoven, Caroline, additional, O'Garra, Anne, additional, O'Leary, Jean D, additional, Olsen, Jessica, additional, O'Neill, Olga, additional, Ordonez Suarez, Paula, additional, O'Reilly, Nicola, additional, Osborne, Neil, additional, Pabari, Amar, additional, Pajak, Aleksandra, additional, Papayannopoulos, Venizelos, additional, Patel, Namita, additional, Patel, Yogen, additional, Paun, Oana, additional, Peat, Nigel, additional, Peces-Barba Castano, Laura, additional, Perez Caballero, Ana, additional, Perez-Lloret, Jimena, additional, Perrault, Magali S, additional, Perrin, Abigail, additional, Poh, Roy, additional, Poirier, Enzo Z, additional, Polke, James M, additional, Pollitt, Marc, additional, Prieto-Godino, Lucia, additional, Proust, Alize, additional, Shah Punatar, Rajvee, additional, Puvirajasinghe, Clinda, additional, Queval, Christophe, additional, Ramachandran, Vijaya, additional, Ramaprasad, Abhinay, additional, Ratcliffe, Peter, additional, Reed, Laura, additional, Reis e Sousa, Caetano, additional, Richardson, Kayleigh, additional, Ridewood, Sophie, additional, Roberts, Rowenna, additional, Rodgers, Angela, additional, Romero Clavijo, Pablo, additional, Rosa, Annachiara, additional, Rossi, Alice, additional, Roustan, Chloe, additional, Rowan, Andrew, additional, Sahai, Erik, additional, Sait, Aaron, additional, Sala, Katarzyna, additional, Sanderson, Theo, additional, Santucci, Pierre, additional, Sardar, Fatima, additional, Sateriale, Adam, additional, Saunders, Jill A, additional, Sawyer, Chelsea, additional, Schlott, Anja, additional, Schweighoffer, Edina, additional, Segura-Bayona, Sandra, additional, Shaw, Joe, additional, Shin, Gee Yen, additional, Silva Dos Santos, Mariana, additional, Silvestre, Margaux, additional, Singer, Matthew, additional, Snell, Daniel M, additional, Song, Ok-Ryul, additional, Steel, Louisa, additional, Strange, Amy, additional, Sullivan, Adrienne E, additional, Tan, Michele SY, additional, Tautz-Davis, Zoe H, additional, Taylor, Effie, additional, Taylor, Gunes, additional, Taylor, Harriet B, additional, Taylor-Beadling, Alison, additional, Teixeira Subtil, Fernanda, additional, Terré Torras, Berta, additional, Toolan-Kerr, Patrick, additional, Torelli, Francesca, additional, Toteva, Tea, additional, Treeck, Moritz, additional, Trojer, Hadija, additional, Tsai, Ming-Han C, additional, Turner, James MA, additional, Turner, Melanie, additional, Ule, Jernej, additional, Ulferts, Rachel, additional, Vanloo, Sharon P, additional, Veeriah, Selvaraju, additional, Venkatesan, Subramanian, additional, Vousden, Karen, additional, Wack, Andreas, additional, Walder, Claire, additional, Walker, Philip A, additional, Wang, Yiran, additional, Ward, Sophia, additional, Wenman, Catharina, additional, Wiliams, Luke, additional, Williams, Matthew J, additional, Wong, Wai Keong, additional, Wright, Joshua, additional, Wu, Mary, additional, Wynne, Lauren, additional, Xiang, Zheng, additional, Yap, Melvyn, additional, Zagalak, Julian A, additional, Zecchin, Davide, additional, Zillwood, Rachel, additional, Matthews, Rebecca, additional, Severn, Abigail, additional, Adam, Sajida, additional, Enfield, Louise, additional, McBride, Angela, additional, Gärtner, Kathleen, additional, Edwards, Sarah, additional, Lorencatto, Fabiana, additional, Michie, Susan, additional, Manley, Ed, additional, Shahmanesh, Maryam, additional, Lukha, Hinal, additional, Prymas, Paulina, additional, McBain, Hazel, additional, Shortman, Robert, additional, Wood, Leigh, additional, Davies, Claudia, additional, Williams, Bethany, additional, Ng, Kevin W, additional, Cornish, Georgina H, additional, Faulkner, Nikhil, additional, Riddell, Andrew, additional, Hobson, Philip, additional, Agua-Doce, Ana, additional, Bartolovic, Kerol, additional, Russell, Emma, additional, Carr, Lotte, additional, Sanchez, Emilie, additional, Frampton, Daniel, additional, Byott, Matthew, additional, Paraskevopoulou, Stavroula M, additional, Crayton, Elise, additional, Meyer, Carly, additional, Gkouleli, Triantafylia, additional, Stoltenberg, Andrea, additional, Ranieri, Veronica, additional, Byrne, Tom, additional, Roberts, Fiona, additional, and Hatipoglu, Emine, additional
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- 2020
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18. Pre-existing and de novo humoral immunity to SARS-CoV-2 in humans
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Ng, Kevin W., primary, Faulkner, Nikhil, additional, Cornish, Georgina H., additional, Rosa, Annachiara, additional, Harvey, Ruth, additional, Hussain, Saira, additional, Ulferts, Rachel, additional, Earl, Christopher, additional, Wrobel, Antoni, additional, Benton, Donald, additional, Roustan, Chloe, additional, Bolland, William, additional, Thompson, Rachael, additional, Agua-Doce, Ana, additional, Hobson, Philip, additional, Heaney, Judith, additional, Rickman, Hannah, additional, Paraskevopoulou, Stavroula, additional, Houlihan, Catherine F., additional, Thomson, Kirsty, additional, Sanchez, Emilie, additional, Brealey, David, additional, Shin, Gee Yen, additional, Spyer, Moira J., additional, Joshi, Dhira, additional, O’Reilly, Nicola, additional, Walker, Philip A., additional, Kjaer, Svend, additional, Riddell, Andrew, additional, Moore, Catherine, additional, Jebson, Bethany R., additional, Wilkinson, Meredyth G.Ll., additional, Marshall, Lucy R., additional, Rosser, Elizabeth C., additional, Radziszewska, Anna, additional, Peckham, Hannah, additional, Ciurtin, Coziana, additional, Wedderburn, Lucy R., additional, Beale, Rupert, additional, Swanton, Charles, additional, Gandhi, Sonia, additional, Stockinger, Brigitta, additional, McCauley, John, additional, Gamblin, Steve, additional, McCoy, Laura E., additional, Cherepanov, Peter, additional, Nastouli, Eleni, additional, and Kassiotis, George, additional
- Published
- 2020
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19. Adapting to the Coronavirus Pandemic: Building and Incorporating a Diagnostic Pipeline in a Shared Resource Laboratory.
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Russell, Emma, Agua‐Doce, Ana, Carr, Lotte, Malla, Asha, Bartolovic, Kerol, Levi, Dina, Henderson, Carl, Das, Debipriya, Rhys, Hefin, Hobson, Philip, Purewal, Sukhveer, and Riddell, Andrew
- Abstract
In March 2020, with lockdown due to the coronavirus pandemic underway, the Francis Crick Institute (the Crick) regeared its research laboratories into clinical testing facilities. Two pipelines were established, one for polymerase chain reaction and the other for Serology. This article discusses the Cricks Flow Cytometry Science Technology Platform (Flow STP) role in setting up the Serology pipeline. Pipeline here referring to the overarching processes in place to facilitate the receipt of human sera through to a SARs‐CoV‐2 enzyme‐linked immunosorbent assay result. We examine the challenges that had to be overcome by a research laboratory to incorporate clinical diagnostics and the processes by which this was achieved. It describes the governance required to run the service, the design of the standard operating procedures (SOPs) and pipeline, the setting up of the assay, the validation required to show the robustness of the pipeline and reporting the results of the assay. Finally, as the lockdown started to ease in June 2020, it examines how this new service affects the daily running of the Flow STP. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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20. T Follicular Regulatory Cells Are Decreased in Patients With Established Treated Rheumatoid Arthritis With Active Disease: Comment on the Article by Liu et al
- Author
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Romão, Vasco C., primary, Fonseca, João Eurico, additional, Agua-Doce, Ana, additional, and Graca, Luis, additional
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- 2018
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21. Reply
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Fonseca, Valter R., primary, Romão, Vasco C., additional, Fonseca, João Eurico, additional, Agua-Doce, Ana, additional, and Graca, Luis, additional
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- 2018
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22. The Ratio of Blood T Follicular Regulatory Cells to T Follicular Helper Cells Marks Ectopic Lymphoid Structure Formation While Activated Follicular Helper T Cells Indicate Disease Activity in Primary Sjögren's Syndrome
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Fonseca, Valter R., primary, Romão, Vasco C., additional, Agua-Doce, Ana, additional, Santos, Mara, additional, López-Presa, Dolores, additional, Ferreira, Ana Cristina, additional, Fonseca, João Eurico, additional, and Graca, Luis, additional
- Published
- 2018
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23. Pandemic peak SARS-CoV-2 infection and seroconversion rates in London frontline health-care workers
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Aitken, Jim, Allen, Zoe, Ambler, Rachel, Ambrose, Karen, Ashton, Emma, Avola, Alida, Balakrishnan, Samutheswari, Barns-Jenkins, Caitlin, Barr, Genevieve, Barrell, Sam, Basu, Souradeep, Beale, Rupert, Beesley, Clare, Bhardwaj, Nisha, Bibi, Shahnaz, Bineva-Todd, Ganka, Biswas, Dhruva, Blackman, Michael J, Bonnet, Dominique, Bowker, Faye, Broncel, Malgorzata, Brooks, Claire, Buck, Michael D, Buckton, Andrew, Budd, Timothy, Burrell, Alana, Busby, Louise, Bussi, Claudio, Butterworth, Simon, Byrne, Fiona, Byrne, Richard, Caidan, Simon, Campbell, Joanna, Canton, Johnathan, Cardoso, Ana, Carter, Nick, Carvalho, Luiz, Carzaniga, Raffaella, Chandler, Natalie, Chen, Qu, Cherepanov, Peter, Churchward, Laura, Clark, Graham, Clayton, Bobbi, Cobolli Gigli, Clementina, Collins, Zena, Cottrell, Sally, Crawford, Margaret, Cubitt, Laura, Cullup, Tom, Davies, Heledd, Davis, Patrick, Davison, Dara, D'Avola, Annalisa, Dearing, Vicky, Debaisieux, Solene, Diaz-Romero, Monica, Dibbs, Alison, Diring, Jessica, Driscoll, Paul C, Earl, Christopher, Edwards, Amelia, Ekin, Chris, Evangelopoulos, Dimitrios, Faraway, Rupert, Fearns, Antony, Ferron, Aaron, Fidanis, Efthymios, Fitz, Dan, Fleming, James, Frederico, Bruno, Gaiba, Alessandra, Gait, Anthony, Gamblin, Steve, Gandhi, Sonia, Gaul, Liam, Golding, Helen M, Goldman, Jacki, Goldstone, Robert, Gomez Dominguez, Belen, Gong, Hui, Grant, Paul R, Greco, Maria, Grobler, Mariana, Guedan, Anabel, Gutierrez, Maximiliano G, Hackett, Fiona, Hall, Ross, Halldorsson, Steinar, Harris, Suzanne, Hashim, Sugera, Healy, Lyn, Heaney, Judith, Herbst, Susanne, Hewitt, Graeme, Higgins, Theresa, Hindmarsh, Steve, Hirani, Rajnika, Hope, Joshua, Horton, Elizabeth, Hoskins, Beth, Houlihan, Catherine F, Howell, Michael, Howitt, Louise, Hoyle, Jacqueline, Htun, Mint R, Hubank, Michael, Huerga Encabo, Hector, Hughes, Deborah, Hughes, Jane, Huseynova, Almaz, Hwang, Ming-Shih, Instrell, Rachael, Jackson, Deborah, Jamal-Hanjani, Mariam, Jenkins, Lucy, Jiang, Ming, Johnson, Mark, Jones, Leigh, Kanu, Nnennaya, Kassiotis, George, Kiely, Louise, King Spert Teixeira, Anastacio, Kirk, Stuart, Kjaer, Svend, Knuepfer, Ellen, Komarov, Nikita, Kotzampaltiris, Paul, Kousis, Konstantinos, Krylova, Tammy, Kucharska, Ania, Labrum, Robyn, Lambe, Catherine, Lappin, Michelle, Lee, Stacey-Ann, Levett, Andrew, Levett, Lisa, Levi, Marcel, Liu, Hon-Wing, Loughlin, Sam, Lu, Wei-Ting, MacRae, James I, Madoo, Akshay, Marczak, Julie A, Martensson, Mimmi, Martinez, Thomas, Marzook, Bishara, Matthews, John, Matz, Joachim M, McCall, Samuel, McCoy, Laura E, McKay, Fiona, McNamara, Edel C, Minutti, Carlos M, Mistry, Gita, Molina-Arcas, Miriam, Montaner, Beatriz, Montgomery, Kylie, Moore, Catherine, Moore, David, Moraiti, Anastasia, Moreira-Teixeira, Lucia, Mukherjee, Joyita, Naceur-Lombardelli, Cristina, Nastouli, Eleni, Nelson, Aileen, Nicod, Jerome, Nightingale, Luke, Nofal, Stephanie, Nurse, Paul, Nutan, Savita, Oedekoven, Caroline, O'Garra, Anne, O'Leary, Jean D, Olsen, Jessica, O'Neill, Olga, Ordonez Suarez, Paula, O'Reilly, Nicola, Osborne, Neil, Pabari, Amar, Pajak, Aleksandra, Papayannopoulos, Venizelos, Patel, Namita, Patel, Yogen, Paun, Oana, Peat, Nigel, Peces-Barba Castano, Laura, Perez Caballero, Ana, Perez-Lloret, Jimena, Perrault, Magali S, Perrin, Abigail, Poh, Roy, Poirier, Enzo Z, Polke, James M, Pollitt, Marc, Prieto-Godino, Lucia, Proust, Alize, Shah Punatar, Rajvee, Puvirajasinghe, Clinda, Queval, Christophe, Ramachandran, Vijaya, Ramaprasad, Abhinay, Ratcliffe, Peter, Reed, Laura, Reis e Sousa, Caetano, Richardson, Kayleigh, Ridewood, Sophie, Roberts, Rowenna, Rodgers, Angela, Romero Clavijo, Pablo, Rosa, Annachiara, Rossi, Alice, Roustan, Chloe, Rowan, Andrew, Sahai, Erik, Sait, Aaron, Sala, Katarzyna, Sanderson, Theo, Santucci, Pierre, Sardar, Fatima, Sateriale, Adam, Saunders, Jill A, Sawyer, Chelsea, Schlott, Anja, Schweighoffer, Edina, Segura-Bayona, Sandra, Shaw, Joe, Shin, Gee Yen, Silva Dos Santos, Mariana, Silvestre, Margaux, Singer, Matthew, Snell, Daniel M, Song, Ok-Ryul, Spyer, Moira J, Steel, Louisa, Strange, Amy, Sullivan, Adrienne E, Swanton, Charles, Tan, Michele SY, Tautz-Davis, Zoe H, Taylor, Effie, Taylor, Gunes, Taylor, Harriet B, Taylor-Beadling, Alison, Teixeira Subtil, Fernanda, Terré Torras, Berta, Toolan-Kerr, Patrick, Torelli, Francesca, Toteva, Tea, Treeck, Moritz, Trojer, Hadija, Tsai, Ming-Han C, Turner, James MA, Turner, Melanie, Ule, Jernej, Ulferts, Rachel, Vanloo, Sharon P, Veeriah, Selvaraju, Venkatesan, Subramanian, Vousden, Karen, Wack, Andreas, Walder, Claire, Walker, Philip A, Wang, Yiran, Ward, Sophia, Wenman, Catharina, Wiliams, Luke, Williams, Matthew J, Wong, Wai Keong, Wright, Joshua, Wu, Mary, Wynne, Lauren, Xiang, Zheng, Yap, Melvyn, Zagalak, Julian A, Zecchin, Davide, Zillwood, Rachel, Matthews, Rebecca, Severn, Abigail, Adam, Sajida, Enfield, Louise, McBride, Angela, Gärtner, Kathleen, Edwards, Sarah, Lorencatto, Fabiana, Michie, Susan, Manley, Ed, Shahmanesh, Maryam, Lukha, Hinal, Prymas, Paulina, McBain, Hazel, Shortman, Robert, Wood, Leigh, Davies, Claudia, Williams, Bethany, Ng, Kevin W, Cornish, Georgina H, Faulkner, Nikhil, Riddell, Andrew, Hobson, Philip, Agua-Doce, Ana, Bartolovic, Kerol, Russell, Emma, Carr, Lotte, Sanchez, Emilie, Frampton, Daniel, Byott, Matthew, Paraskevopoulou, Stavroula M, Crayton, Elise, Meyer, Carly, Vora, Nina, Gkouleli, Triantafylia, Stoltenberg, Andrea, Ranieri, Veronica, Byrne, Tom, Lewer, Dan, Hayward, Andrew, Gilson, Richard, Kelly, Gavin, Roberts, Fiona, Hatipoglu, Emine, and Byrne, Thomas
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- 2020
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24. Route of Antigen Presentation Can Determine the Selection of Foxp3-Dependent or Foxp3-Independent Dominant Immune Tolerance
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Agua-Doce, Ana, primary, Caridade, Marta, additional, Oliveira, Vanessa G., additional, Bergman, Lisa, additional, Lafaille, Maria C., additional, Lafaille, Juan J., additional, Demengeot, Jocelyne, additional, and Graca, Luis, additional
- Published
- 2018
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25. Human blood T fr cells are indicators of ongoing humoral activity not fully licensed with suppressive function
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Fonseca, Valter R., primary, Agua-Doce, Ana, additional, Maceiras, Ana Raquel, additional, Pierson, Wim, additional, Ribeiro, Filipa, additional, Romão, Vasco C., additional, Pires, Ana Rita, additional, da Silva, Susana Lopes, additional, Fonseca, João Eurico, additional, Sousa, Ana E., additional, Linterman, Michelle A., additional, and Graca, Luis, additional
- Published
- 2017
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26. Regulatory T Cells and the Control of the Allergic Response
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Agua-Doce, Ana and Graca, Luis
- Subjects
Article Subject ,chemical and pharmacologic phenomena - Abstract
The study of immune regulation and tolerance has been traditionally associated with self/nonself-discrimination. However, the finding that dominant tolerance, a model that puts in evidence the active role of regulatory T cells, can develop to nonself-antigens suggests that the imposition of tolerance can be context dependent. This paper reviews the emerging field of acquired immune tolerance to non-self antigens, with an emphasis on the different subsets of induced regulatory T cells that appear to specialize in specific functional niches. Such regulatory mechanisms are important in preventing the onset of allergic diseases in healthy individuals. In addition, it may be possible to take advantage of these immune regulatory mechanisms for the induction of tolerance in cases where pathological immune responses are generated to allergens occurring in nature, but also to other immunogens such as biological drugs developed for medical therapies.
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- 2012
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27. IL-9 Expression by Invariant NKT Cells Is Not Imprinted during Thymic Development
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Monteiro, Marta, primary, Agua-Doce, Ana, additional, Almeida, Catarina F., additional, Fonseca-Pereira, Diogo, additional, Veiga-Fernandes, Henrique, additional, and Graca, Luis, additional
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- 2015
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28. Human blood Tfr cells are indicators of ongoing humoral activity not fully licensed with suppressive function.
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Fonseca, Valter R., Agua-Doce, Ana, Maceiras, Ana Raquel, Pierson, Wim, Ribeiro, Filipa, Romão, Vasco C., Pires, Ana Rita, da Silva, Susana Lopes, Fonseca, João Eurico, Sousa, Ana E., Linterman, Michelle A., and Graca, Luis
- Abstract
Germinal center (GC) responses are controlled by T follicular helper (T
fh ) and T follicular regulatory (Tfr ) cells and are crucial for the generation of high-affinity antibodies. Although the biology of human circulating and tissue Tfh cells has been established, the relationship between blood and tissue Tfr cells defined as CXCR5+ Foxp3+ T cells remains elusive. We found that blood Tfr cells are increased in Sjögren syndrome, an autoimmune disease with ongoing GC reactions, especially in patients with high autoantibody titers, as well as in healthy individuals upon influenza vaccination. Although blood Tfr cells correlated with humoral responses, they lack full B cell–suppressive capacity, despite being able to suppress T cell proliferation. Blood Tfr cells have a naïve-like phenotype, although they are absent from human thymus or cord blood. We found that these cells were generated in peripheral lymphoid tissues before T-B interaction, as they are maintained in B cell–deficient patients. Therefore, blood CXCR5+ Foxp3+ T cells in human pathology indicate ongoing humoral activity but are not fully competent circulating Tfr cells. [ABSTRACT FROM AUTHOR]- Published
- 2017
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29. The fate of CD4+T cells under tolerance‐inducing stimulation: a modeling perspective
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Caridade, Marta, primary, Oliveira, Vanessa G, additional, Agua‐Doce, Ana, additional, Graca, Luis, additional, and Ribeiro, Ruy M, additional
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- 2013
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30. Induced IL-17–Producing Invariant NKT Cells Require Activation in Presence of TGF-β and IL-1β
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Monteiro, Marta, primary, Almeida, Catarina F., additional, Agua-Doce, Ana, additional, and Graca, Luis, additional
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- 2013
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31. T Cell Apoptosis and Induction of Foxp3+ Regulatory T Cells Underlie the Therapeutic Efficacy of CD4 Blockade in Experimental Autoimmune Encephalomyelitis
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Duarte, Joana, primary, Carrié, Nadège, additional, Oliveira, Vanessa G., additional, Almeida, Catarina, additional, Agua-Doce, Ana, additional, Rodrigues, Lénia, additional, Simas, J. Pedro, additional, Mars, Lennart T., additional, and Graca, Luis, additional
- Published
- 2012
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32. Regulation of the Germinal Center Reaction by Foxp3+ Follicular Regulatory T Cells
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Wollenberg, Ivonne, primary, Agua-Doce, Ana, additional, Hernández, Andrea, additional, Almeida, Catarina, additional, Oliveira, Vanessa G., additional, Faro, Jose, additional, and Graca, Luis, additional
- Published
- 2011
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33. Prevention of House Dust Mite Induced Allergic Airways Disease in Mice through Immune Tolerance
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Agua-Doce, Ana, primary and Graca, Luis, additional
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- 2011
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34. Identification of Regulatory Foxp3+ Invariant NKT Cells Induced by TGF-β
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Monteiro, Marta, primary, Almeida, Catarina F., additional, Caridade, Marta, additional, Ribot, Julie C., additional, Duarte, Joana, additional, Agua-Doce, Ana, additional, Wollenberg, Ivonne, additional, Silva-Santos, Bruno, additional, and Graca, Luis, additional
- Published
- 2010
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35. Modulation of IL-17 and Foxp3 Expression in the Prevention of Autoimmune Arthritis in Mice
- Author
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Duarte, Joana, primary, Agua-Doce, Ana, additional, Oliveira, Vanessa G., additional, Fonseca, João Eurico, additional, and Graca, Luis, additional
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- 2010
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36. The fate of CD4+ T cells under tolerance-inducing stimulation: a modeling perspective.
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Caridade, Marta, Oliveira, Vanessa G, Agua‐Doce, Ana, Graca, Luis, and Ribeiro, Ruy M
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CD4 antigen ,T cells ,MONOCLONAL antibodies ,ANIMAL models in research ,AUTOIMMUNITY ,CELL death ,CELL proliferation ,APOPTOSIS - Abstract
Non-depleting anti-CD4 monoclonal antibodies (MAbs) induce long-term dominant tolerance mediated by regulatory T cells in several animal models of transplantation, allergy and autoimmunity. However, despite many studies on tolerance induction following CD4 blockade, the consequences of this intervention on T-cell kinetics are still unknown. Mathematical models have been useful to understand lymphocyte dynamics, estimating rates of proliferation and cell death following an intervention. Using the same strategy, we found that CD4
+ T cells activated in vitro in the presence of non-depleting anti-CD4 MAbs are prevented from undergoing optimal proliferation and show a higher frequency of apoptosis. Although the changes are small, during the course of a proliferative response, they lead to very distinct final levels of cell numbers. The importance of these mechanisms, predicted by the mathematical model, was validated by showing that lck-driven Bcl-xL transgenic mice, bearing T cells resistant to apoptosis, fail to become tolerant to skin grafts following CD4-blockade. Our data show that, in addition to induction of regulatory T cells, CD4 blockade has a marked effect in the effector T-cell pool by the combined action of hindering proliferation while favoring apoptosis. It is, therefore, the combination of all those mechanisms that leads to stable tolerance. [ABSTRACT FROM AUTHOR]- Published
- 2013
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37. Regulation of the Germinal Center Reaction by Foxp3+ Follicular Regulatory T Cells.
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Wollenberg, Ivonne, Agua-Doce, Ana, Hernández, Andrea, Almeida, Catarina, Oliveira, Vanessa G., Faro, Jose, and Graca, Luis
- Subjects
- *
GERMINAL centers , *FORKHEAD transcription factors , *T cells , *B cells , *IMMUNE response - Abstract
Follicular helper T (TFH) cells participate in humoral responses providing selection signals to germinal center B cells. Recently, expression of CXCR5, PD-1, and the transcription factor Bcl-6 has allowed the identification of TFH cells. We found that a proportion of follicular T cells, with phenotypic characteristics of TFH cells and expressing Foxp3, are recruited during the course of a germinal center (GC) reaction. These Foxp3+ cells derive from natural regulatory T cells. To establish the in vivo physiologic importance of Foxp3+ follicular T cells, we used CXCR5-deficient Foxp3+ cells, which do not have access to the follicular region. Adoptive cell transfers of CXCR5-deficient Foxp3+ cells have shown that Foxp3+ follicular T cells are important regulators of the GC reaction following immunization with a thymus-dependent Ag. Our in vivo data show that Foxp3+ follicular T cells can limit the magnitude of the GC reaction and also the amount of secreted Ag-specific IgM, IgG1, IgG2b, and IgA. Therefore, Foxp3+ follicular regulatory T cells appear to combine characteristics of TFH and regulatory T cells for the control of humoral immune responses. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
38. Monoclonal anti-CD8 therapy induces disease amelioration in the K/BxN mouse model of spontaneous chronic polyarthritis.
- Author
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Raposo, Bruno R, Rodrigues-Santos, Paulo, Carvalheiro, Helena, Agua-Doce, Ana M, Carvalho, Lina, Pereira da Silva, José A, Graça, Luis, and Souto-Carneiro, M Margarida
- Abstract
OBJECTIVE: CD8+ T cells are part of the T cell pool infiltrating the synovium in rheumatoid arthritis (RA). However, their role in the pathogenesis of RA has not been fully delineated. Using the K/BxN mouse model of spontaneous chronic arthritis, which shares many similarities with RA, we studied the potential of CD8+ T cell depletion with monoclonal antibodies (mAb) to stop and reverse the progression of experimental arthritis. METHODS: CD8+ T cells from the blood and articular infiltrate of K/BxN mice were characterized for cell surface phenotypic markers and for cytokine production. Additionally, mice were treated with specific anti-CD8 mAb (YTS105 and YTS169.4), with and without thymectomy. RESULTS: CD8+ T cells from the peripheral blood and joints of K/BxN mice were mainly CD69+ and CD62L-CD27+ T cells expressing proinflammatory cytokines (interferon-[gamma] [IFN[gamma]], tumor necrosis factor [alpha] [TNF[alpha]], interleukin-17a [IL-17A], and IL-4), and granzyme B. In mice receiving anti-CD8 mAb, the arthritis score improved 5 days after treatment. Recovery of the CD8+ T cells was associated with a new increase in the arthritis score after 20 days. In thymectomized and anti-CD8 mAb-treated mice, the arthritis score improved permanently. Histologic analysis showed an absence of inflammatory infiltrate in the anti-CD8 mAb-treated mice. In anti-CD8 mAb-treated mice, the serologic levels of TNF[alpha], IFN[gamma], IL-6, and IL-5 normalized. The levels of the disease-related anti-glucose-6-phosphate isomerase antibodies did not change. CONCLUSION: These results indicate that synovial activated effector CD8+ T cells locally synthesize proinflammatory cytokines (IFN[gamma], TNF[alpha], IL-17, IL-6) and granzyme B in the arthritic joint, thus playing a pivotal role in maintaining chronic synovitis in the K/BxN mouse model of arthritis. [ABSTRACT FROM AUTHOR]
- Published
- 2010
39. The role of human umbilical cord tissue-derived mesenchymal stromal cells (UCX®) in the treatment of inflammatory arthritis.
- Author
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Santos, Jorge M, Bárcia, Rita N, Simoes, Sandra I, Gaspar, Manuela M, Calado, Susana, Agua-Doce, Ana, Almeida, Sílvia Cp, Almeida, Joana, Filipe, Mariana, Teixeira, Mariana, Martins, José P, Graça, Luís, Cruz, Maria Em, Cruz, Pedro, Cruz, Helder, Simões, Sandra I, Almeida, Sílvia C P, and Cruz, Maria E M
- Abstract
Background: ECBio has developed proprietary technology to consistently isolate, expand and cryopreserve a well-characterized population of stromal cells from human umbilical cord tissue (UCX® cells). The technology has recently been optimized in order to become compliant with Advanced Medicine Therapeutic Products. In this work we report the immunosuppressive capacity of UCX® cells for treating induced autoimmune inflammatory arthritis.Methods: UCX® cells were isolated using a proprietary method (PCT/IB2008/054067) that yields a well-defined number of cells using a precise proportion between tissue digestion enzyme activity units, tissue mass, digestion solution volume and void volume. The procedure includes three recovery steps to avoid non-conformities related to cell recovery. UCX® surface markers were characterized by flow cytometry and UCX® capacity to expand in vitro and to differentiate into adipocyte, chondrocyte and osteoblast-like cells was evaluated. Mixed Lymphocyte Reaction (MLR) assays were performed to evaluate the effect of UCX® cells on T-cell activation and Treg conversion assays were also performed in vitro. Furthermore, UCX® cells were administered in vivo in both a rat acute carrageenan-induced arthritis model and rat chronic adjuvant induced arthritis model for arthritic inflammation. UCX® anti-inflammatory activity was then monitored over time.Results: UCX® cells stained positive for CD44, CD73, CD90 and CD105; and negative for CD14, CD19 CD31, CD34, CD45 and HLA-DR; and were capable to differentiate into adipocyte, chondrocyte and osteoblast-like cells. UCX® cells were shown to repress T-cell activation and promote the expansion of Tregs better than bone marrow mesenchymal stem cells (BM-MSCs). Accordingly, xenogeneic UCX® administration in an acute carrageenan-induced arthritis model showed that human UCX® cells can reduce paw edema in vivo more efficiently than BM-MSCs. Finally, in a chronic adjuvant induced arthritis model, animals treated with intra-articular (i.a.) and intra-peritoneal (i.p.) infusions of UCX® cells showed faster remission of local and systemic arthritic manifestations.Conclusion: The results suggest that UCX® cells may be an effective and promising new approach for treating both local and systemic manifestations of inflammatory arthritis. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
40. Induced IL- 17-Producing Invariant NKT Cells Require Activation in Presence of TGF-ß and IL-lß.
- Author
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Monteiro, Marta, Almeida, Catarina F., Agua-Doce, Ana, and Graca, Luis
- Subjects
- *
INTERLEUKIN-17 , *KILLER cells , *TRANSFORMING growth factors , *NATURAL immunity , *LYMPHOCYTES , *THYMUS , *CELL differentiation - Abstract
IL-17 productio n by innate-like lymphocytes, including y5 and invariant NKT (iNKT) cells, have been ascribed to specific lineages that are endowed with this functional specialization during thymic differentiation. IL-17-producing iNKT cells have been de-scribed as a CD4-NK1.1- lineage in mice and CD161+ in humans. We found that, in mice, noncommitted iNKT cells can be induced to produce IL-17 when activated in presence of TGF-ß and IL-lß. This peripheral induction of IL-17 expression could be observed in any subset irrespectively of CD4 and NK1.1 expression, the process leading to loss of NK1.1 expression and partial CD4 downmodulation. Furthermore, induced IL-17-producing iNKT cells were sufficient to drive neutrophilic airways inflam-mation upon intratracheal adoptive cell transfer into congenie mice. Taken together, our data show that similarly to regulatory T cells, which have a natural and peripherally induced subset, IL-17 production by iNKT cells can also be imprinted in natural ÍNKT17 cells or peripherally induced. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
41. T Cell Apoptosis and Induction of Foxp3+ Regulatory T Cells Underlie the Therapeutic Efficacy of CD4 Blockade in Experimental Autoimmune Encephalomyelitis.
- Author
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Duarte, Joana, Carrié, Nadège, Oliveira, Vanessa G., Almeida, Catarina, Agua-Doce, Ana, Rodrigues, Lénia, Simas, J. Pedro, Mars, Lennart T., and Graca, Luis
- Subjects
- *
IMMUNOLOGICAL aspects of encephalomyelitis , *T cells , *APOPTOSIS , *FORKHEAD transcription factors , *CD4 antigen , *AUTOIMMUNE diseases , *MULTIPLE sclerosis , *PHENOTYPES - Abstract
The pathogenesis of multiple sclerosis requires the participation of effector neuroantigen-specific T cells. Thus, T cell targeting has been proposed as a promising therapeutic strategy. However, the mechanism underlying effective disease prevention following T cell targeting remains incompletely known. We found, using several TCR-transgenic strains, that CD4 blockade is effective in preventing experimental autoimmune encephalopathy and in treating mice after the disease onset. The mechanism does not rely on direct T cell depletion, but the anti-CD4 mAb prevents the proliferation of naive neuroantigen-specific T cells, as well as acquisition of effector Thl and Thl7 phenotypes. Simultaneously, the mAb favors peripheral conversion of Foxp3+ regulatory T cells. Pre-existing effector cells, or neuroantigen-specific cells that undergo cell division despite the presence of anti-CD4, are committed to apoptosis. Therefore, protection from experimental autoimmune encephalopathy relies on a combination of dominant mechanisms grounded on regulatory T cell induction and recessive mechanisms based on apoptosis of neuropathogenic cells. We anticipate that the same mechanisms may be implicated in other T cell-mediated autoimmune diseases that can be treated or prevented with Abs targeting T cell molecules, such as CD4 or CD3. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
42. Functional antibody and T-cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study.
- Author
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Fendler A, Au L, Shepherd STC, Byrne F, Cerrone M, Boos LA, Rzeniewicz K, Gordon W, Shum B, Gerard CL, Ward B, Xie W, Schmitt AM, Joharatnam-Hogan N, Cornish GH, Pule M, Mekkaoui L, Ng KW, Carlyle E, Edmonds K, Del Rosario L, Sarker S, Lingard K, Mangwende M, Holt L, Ahmod H, Stone R, Gomes C, Flynn HR, Agua-Doce A, Hobson P, Caidan S, Howell M, Wu M, Goldstone R, Crawford M, Cubitt L, Patel H, Gavrielides M, Nye E, Snijders AP, MacRae JI, Nicod J, Gronthoud F, Shea RL, Messiou C, Cunningham D, Chau I, Starling N, Turner N, Welsh L, van As N, Jones RL, Droney J, Banerjee S, Tatham KC, Jhanji S, O'Brien M, Curtis O, Harrington K, Bhide S, Bazin J, Robinson A, Stephenson C, Slattery T, Khan Y, Tippu Z, Leslie I, Gennatas S, Okines A, Reid A, Young K, Furness AJS, Pickering L, Gandhi S, Gamblin S, Swanton C, Nicholson E, Kumar S, Yousaf N, Wilkinson KA, Swerdlow A, Harvey R, Kassiotis G, Larkin J, Wilkinson RJ, and Turajlic S
- Abstract
Patients with cancer have higher COVID-19 morbidity and mortality. Here we present the prospective CAPTURE study (NCT03226886) integrating longitudinal immune profiling with clinical annotation. Of 357 patients with cancer, 118 were SARS-CoV-2-positive, 94 were symptomatic and 2 patients died of COVID-19. In this cohort, 83% patients had S1-reactive antibodies, 82% had neutralizing antibodies against WT, whereas neutralizing antibody titers (NAbT) against the Alpha, Beta, and Delta variants were substantially reduced. Whereas S1-reactive antibody levels decreased in 13% of patients, NAbT remained stable up to 329 days. Patients also had detectable SARS-CoV-2-specific T cells and CD4+ responses correlating with S1-reactive antibody levels, although patients with hematological malignancies had impaired immune responses that were disease and treatment-specific, but presented compensatory cellular responses, further supported by clinical. Overall, these findings advance the understanding of the nature and duration of immune response to SARS-CoV-2 in patients with cancer.
- Published
- 2021
- Full Text
- View/download PDF
43. Identification of regulatory Foxp3+ invariant NKT cells induced by TGF-beta.
- Author
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Monteiro M, Almeida CF, Caridade M, Ribot JC, Duarte J, Agua-Doce A, Wollenberg I, Silva-Santos B, and Graca L
- Subjects
- Animals, Cell Differentiation drug effects, Cell Differentiation immunology, Cell Movement immunology, Cells, Cultured, Encephalomyelitis, Autoimmune, Experimental immunology, Encephalomyelitis, Autoimmune, Experimental metabolism, Encephalomyelitis, Autoimmune, Experimental prevention & control, Female, Flow Cytometry, Forkhead Transcription Factors metabolism, Galactosylceramides immunology, Galactosylceramides pharmacology, Liver immunology, Liver metabolism, Lymph Nodes immunology, Lymph Nodes metabolism, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Natural Killer T-Cells metabolism, T-Lymphocytes, Regulatory metabolism, Transforming Growth Factor beta immunology, Transforming Growth Factor beta metabolism, Forkhead Transcription Factors immunology, Natural Killer T-Cells immunology, T-Lymphocytes, Regulatory immunology, Transforming Growth Factor beta pharmacology
- Abstract
Invariant NKT (iNKT) cells were shown to prevent the onset of experimental autoimmune encephalomyelitis in mice following administration of their specific TCR agonist alpha-galactosylceramide. We found that this protection was associated with the emergence of a Foxp3(+) iNKT cell population in cervical lymph nodes. We demonstrate that the differentiation of these cells is critically dependent on TGF-beta in both mice and humans. Moreover, in vivo generation of Foxp3(+) iNKT cells was observed in the TGF-beta-rich environment of the murine gut. Foxp3(+) iNKT cells displayed a phenotype similar to that of Foxp3(+) regulatory T cells, and they suppress through a contact-dependent, glucocorticoid-induced TNFR-mediated mechanism. Nevertheless, Foxp3(+) iNKT cells retain distinctive NKT cell characteristics, such as promyelocytic leukemia zinc finger protein expression and preferential homing to the liver following adoptive transfer, where they stably maintained Foxp3 expression. Our data thus unveil an unexpected capacity of iNKT cells to acquire regulatory functions that may contribute to the establishment of immunological tolerance.
- Published
- 2010
- Full Text
- View/download PDF
44. B cells from the bench to the clinical practice.
- Author
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Moura R, Agua-Doce A, Weinmann P, Graça L, and Fonseca JE
- Subjects
- Antibody Formation, Antigens immunology, Autoimmunity, Humans, Lymphocyte Activation, B-Lymphocytes immunology
- Abstract
B cells develop in bone marrow and undergo antigen-induced activation and terminal differentiation in germinal centres of secondary lymphoid organs. Each B cell is a clone which means that an individual B cell has a unique genetic code and produces only one type of antibody when stimulated by antigen being able to multiply itself and originate several B cells with the same antigen specificity (clonal selection theory). However their important role in adaptive immune responses is supported by the remarkable capacity of recognizing an unlimited array of antigens due to mechanisms of antibody diversity such as V(D)J recombination class switching and somatic hypermutation. B cells can also function as antigen presenting cells that can activate T cells improving the effectiveness of the immune response. Immune B cell tolerance surveillance through clonal deletion anergy and receptor editing is also necessary to avoid pathological conditions like autoimmune diseases. B cells can contribute to autoimmunity by autoantibody production cytokine synthesis antigen presentation T cell activation and ectopic lymphogenesis.
- Published
- 2008
45. Induction of dominant tolerance using monoclonal antibodies.
- Author
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Agua-Doce A and Graça L
- Subjects
- Animals, Autoimmune Diseases immunology, Autoimmune Diseases therapy, Immunotherapy methods, T-Lymphocytes, Regulatory immunology, Transplantation Tolerance immunology, Antibodies, Monoclonal therapeutic use, Immune Tolerance immunology
- Abstract
Monoclonal antibodies (MAb) have been shown to be effective in inducing immune tolerance in transplantation and autoimmunity. Several different MAb have tolerogenic properties and their effect has been studied in a range of experimental animal models and, in some cases, in clinical trials. The tolerant state seems to be maintained by CD4+ regulatory T cells (Treg), induced in the periphery, capable of suppressing other T cells specific for the same antigens or antigens presented by the same antigen presenting cells. Furthermore, following the initial induction of Treg cells under MAb treatment, Treg cells themselves can maintain the tolerant state in a dominant way in the absence of the therapeutic MAb or other immunosuppressive agents, and are able to recruit other T cells into the regulatory pool--a process named infectious tolerance.
- Published
- 2007
- Full Text
- View/download PDF
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