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22 results on '"Ahern, Kevin R"'

3. The maize W22 genome provides a foundation for functional genomics and transposon biology

Author

Springer, Nathan M., Anderson, Sarah N., Andorf, Carson M., Ahern, Kevin R., Bai, Fang, Barad, Omer, Barbazuk, W. Brad, Bass, Hank W., Baruch, Kobi, Ben-Zvi, Gil, Buckler, Edward S., Bukowski, Robert, Campbell, Michael S., Cannon, Ethalinda K. S., Chomet, Paul, Dawe, R. Kelly, Davenport, Ruth, Dooner, Hugo K., Du, Limei He, Du, Chunguang, Easterling, Katherine A., Gault, Christine, Guan, Jiahn-Chou, Hunter, Charles T., Jander, Georg, Jiao, Yinping, Koch, Karen E., Kol, Guy, Köllner, Tobias G., Kudo, Toru, Li, Qing, Lu, Fei, Mayfield-Jones, Dustin, Mei, Wenbin, McCarty, Donald R., Noshay, Jaclyn M., Portwood, II, John L., Ronen, Gil, Settles, A. Mark, Shem-Tov, Doron, Shi, Jinghua, Soifer, Ilya, Stein, Joshua C., Stitzer, Michelle C., Suzuki, Masaharu, Vera, Daniel L., Vollbrecht, Erik, Vrebalov, Julia T., Ware, Doreen, Wei, Sharon, Wimalanathan, Kokulapalan, Woodhouse, Margaret R., Xiong, Wenwei, and Brutnell, Thomas P.

Published
2018
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5. Biosynthesis of 8- O -Methylated Benzoxazinoid Defense Compounds in Maize

Author

Handrick, Vinzenz, Robert, Christelle A.M., Ahern, Kevin R., Zhou, Shaoqun, Machado, Ricardo A.R., Maag, Daniel, Glauser, Gaetan, Fernandez-Penny, Felix E., Chandran, Jima N., Rodgers-Melnik, Eli, Schneider, Bernd, Buckler, Edward S., Boland, Wilhelm, Gershenzon, Jonathan, Jander, Georg, Erb, Matthias, and Köllner, Tobias G.

Published
2016

9. Genome-Wide Distribution of Transposed Dissociation Elements in Maize

Author

Vollbrecht, Erik, Duvick, Jon, Schares, Justin P., Ahern, Kevin R., Deewatthanawong, Prasit, Xu, Ling, Conrad, Liza J., Kikuchi, Kazuhiro, Kubinec, Tammy A., Hall, Bradford D., Weeks, Rebecca, Unger-Wallace, Erica, Muszynski, Michael, Brendel, Volker P., and Brutnell, Thomas P.

Published
2010

11. Distribution of Activator (Ac) throughout the maize genome for use in regional mutagenesis

Author

Kolkman, Judith M., Conrad, Liza J., Farmer, Phyllis R., Hardeman, Kristine, Ahern, Kevin R., Lewis, Paul E., Sawers, Ruairidh J.H., Lebejko, Sara, Chomet, Paul, and Brutnell, Thomas P.

Subjects
Corn -- Genetic aspects, Genetic research, Genomics, Biological sciences
Abstract

A collection of Activator (Ac)-containing, near-isogenic W22 inbred lines has been generated for use in regional mutagenesis experiments. Each line is homozygous for a single, precisely positioned Ac element and the Ds reporter, r1-sc:m3. Through classical and molecular genetic techniques, 158 transposed Ac elements (tr-Acs) were distributed throughout the maize genome and 41 were precisely placed on the linkage map utilizing multiple recombinant inbred populations. Several PCR techniques were utilized to amplify DNA fragments flanking tr-Ac insertions up to 8 kb in length. Sequencing and database searches of flanking DNA revealed that the majority of insertions are in hypomethylated, low- or single-copy sequences, indicating an insertion site preference for genic sequences in the genome. However, a number of Ac transposition events were to highly repetitive sequences in the genome. We present evidence that suggests Ac expression is regulated by genomic context resulting in subtle variations in Ac-mediated excision patterns. These tr-Ac lines can be utilized to isolate genes with unknown function, to conduct fine-scale genetic mapping experiments, and to generate novel allelic diversity in applied breeding programs.

Published
2005

13. An N-acetylglucosamine transporter required for arbuscular mycorrhizal symbioses in rice and maize

Author

Nadal, Marina, Sawers, Ruairidh, Naseem, Shamoon, Bassin, Barbara, Kulicke, Corinna, Sharman, Abigail, An, Gynheung, An, Kyungsook, Ahern, Kevin R, Romag, Amanda, Brutnell, Thomas P, Gutjahr, Caroline, Geldner, Niko, Roux, Christophe, Martinoia, Enrico, Konopka, James B, Paszkowski, Uta, University of Zurich, Paszkowski, Uta, Paszkowski, Uta [0000-0002-7279-7632], and Apollo - University of Cambridge Repository

Subjects
food and beverages, Oryza, 580 Plants (Botany), Genes, Plant, Plant Roots, Zea mays, 10126 Department of Plant and Microbial Biology, Mycorrhizae, Mutation, 1110 Plant Science, Cloning, Molecular, 10211 Zurich-Basel Plant Science Center, Phosphoenolpyruvate Sugar Phosphotransferase System, Symbiosis, Signal Transduction
Abstract

Most terrestrial plants, including crops, engage in beneficial interactions with arbuscular mycorrhizal fungi. Vital to the association is mutual recognition involving the release of diffusible signals into the rhizosphere. Previously, we identified the maize $\textit{no perception 1}$ ($\textit{nope1}$) mutant to be defective in early signalling. Here, we report cloning of $\textit{ZmNope1}$ on the basis of synteny with rice. $\textit{NOPE1}$ encodes a functional homologue of the $\textit{Candida albicans}$ $N$-acetylglucosamine (GlcNAc) transporter $\textit{NGT1}$, and represents the first plasma membrane GlcNAc transporter identified from plants. In $\textit{C. albicans}$, exposure to GlcNAc activates cell signalling and virulence. Similarly, in $\textit{Rhizophagus irregularis}$ treatment with rice wild-type but not $\textit{nope1}$ root exudates induced transcriptome changes associated with signalling function, suggesting a requirement of NOPE1 function for presymbiotic fungal reprogramming.

Published
2017

14. Metabolome-scale genome-wide association studies reveal chemical diversity and genetic control of maize specialized metabolites

Author

Shaoqun, Zhou, primary, Kremling, Karl A., additional, Nonoy, Bandillo, additional, Annett, Richter, additional, Zhang, Ying K., additional, Ahern, Kevin R., additional, Artyukhin, Alexander B., additional, Hui, Joshua X., additional, Schroeder, Frank C., additional, Buckler, Edward S., additional, and Georg, Jander, additional

Published
2018
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15. An N-acetylglucosamine transporter required for arbuscular mycorrhizal symbioses in rice and maize

Author

Nadal, Marina, primary, Sawers, Ruairidh, additional, Naseem, Shamoon, additional, Bassin, Barbara, additional, Kulicke, Corinna, additional, Sharman, Abigail, additional, An, Gynheung, additional, An, Kyungsook, additional, Ahern, Kevin R., additional, Romag, Amanda, additional, Brutnell, Thomas P., additional, Gutjahr, Caroline, additional, Geldner, Niko, additional, Roux, Christophe, additional, Martinoia, Enrico, additional, Konopka, James B., additional, and Paszkowski, Uta, additional

Published
2017
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20. Dynamic Maize Responses to Aphid Feeding Are Revealed by a Time Series of Transcriptomic and Metabolomic Assays

Author

0000-0002-6489-5566, Tzin, Vered, Fernández-Pozo, Noé, Richter, Annett, Schmelz, Eric A., Schoettner, Matthias, Schäfer, Martin, Ahern, Kevin R., Meihls, Lisa N., Kaur, Harleen, Huffaker, Alisa, Mori, Naoki, Degenhardt, Joerg, Mueller, Lukas A., Jander, Georg, 0000-0002-6489-5566, Tzin, Vered, Fernández-Pozo, Noé, Richter, Annett, Schmelz, Eric A., Schoettner, Matthias, Schäfer, Martin, Ahern, Kevin R., Meihls, Lisa N., Kaur, Harleen, Huffaker, Alisa, Mori, Naoki, Degenhardt, Joerg, Mueller, Lukas A., and Jander, Georg

Abstract

As a response to insect attack, maize (Zea mays) has inducible defenses that involve large changes in gene expression and metabolism. Piercing/sucking insects such as corn leaf aphid (Rhopalosiphum maidis) cause direct damage by acquiring phloem nutrients as well as indirect damage through the transmission of plant viruses. To elucidate the metabolic processes and gene expression changes involved in maize responses to aphid attack, leaves of inbred line B73 were infested with corn leaf aphids for 2 to 96 h. Analysis of infested maize leaves showed two distinct response phases, with the most significant transcriptional and metabolic changes occurring in the first few hours after the initiation of aphid feeding. After 4 d, both gene expression and metabolite profiles of aphid-infested maize reverted to being more similar to those of control plants. Although there was a predominant effect of salicylic acid regulation, gene expression changes also indicated prolonged induction of oxylipins, although not necessarily jasmonic acid, in aphid-infested maize. The role of specific metabolic pathways was confirmed using Dissociator transposon insertions in maize inbred line W22. Mutations in three benzoxazinoid biosynthesis genes, Bx1, Bx2, and Bx6, increased aphid reproduction. In contrast, progeny production was greatly decreased by a transposon insertion in the single W22 homolog of the previously uncharacterized B73 terpene synthases TPS2 and TPS3. Together, these results show that maize leaves shift to implementation of physical and chemical defenses within hours after the initiation of aphid feeding and that the production of specific metabolites can have major effects in maize-aphid interactions.

Published
2015

22. Genome-Wide Distribution of TransposedDissociationElements in Maize

Author

Vollbrecht, Erik, primary, Duvick, Jon, additional, Schares, Justin P., additional, Ahern, Kevin R., additional, Deewatthanawong, Prasit, additional, Xu, Ling, additional, Conrad, Liza J., additional, Kikuchi, Kazuhiro, additional, Kubinec, Tammy A., additional, Hall, Bradford D., additional, Weeks, Rebecca, additional, Unger-Wallace, Erica, additional, Muszynski, Michael, additional, Brendel, Volker P., additional, and Brutnell, Thomas P., additional

Published
2010
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