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Additional file 1: Fig. S1. The salt stress and recovery condition of A. littoralis and their growth parameters.

Additional file 1: Table S1. Primers used in this study. Table S2. Nucleic acid and protein impurities in purified HA samples. Figure S1. The full length hasA expression cassette. Figure S2. PCR products gel electrophoresis of full-length and truncated form of has A. Figure S3. The truncated form of Tr4 expression cassette. Figure S4. The truncated Tr3 expression cassette. Figure S5. Gel electrophoresis of colony PCR on recombinant strains. Figure S6. The cell growth and HA production plots. Figure S7. The data of GelAnalyzer software for determination of purified HA Mw

Additional file 2: Fig. S2. Experiment timeline.

Additional file 4: Fig. S4. A leaf surface of A. littoralis plants grown at different watering regimes (left: Hoagland solution, and right: On the leaves of a stressed plant, salt gland secretions were visible in the form of salt crystals.

In this paper, we have synthesized a novel chitosan-p-tert-butylcalix[4]arene polymer (CCP) as a highly efficient adsorbent for mercury ion (Hg2+) removal from water. In fact, a lower rim diamine derivative of p-tert-butylcalix[4]arene has been cross-linked with chitosan chain by carbonyl diimidazole (CDI) as the linker. CDI forms an urea linkage between calix[4]arene diamine derivative and amine groups of the chitosan polymeric chain. The structure and properties of the new polymer were characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscope. Also, the adsorption capacity of CCP was studied towards Hg2+ in aqueous medium by inductively coupled plasma-optical emission spectrometry. Interestingly, the results showed a considerable adsorption capacity for CCP in comparison with chitosan. Therefore, CCP can be introduced as a promising adsorbent for the elimination of Hg2+ from wastewaters. Moreover, because of the conformity of adsorption kinetic with pseudo-second-order kinetic models, it can be concluded that chemical adsorption has an important role between functional groups on CCP polymer and Hg2+ ions. In addition, according to Freundlich isotherm, CCP surface was heterogeneous with different functional groups.

Additional file 1. The schematic view of the pET26 plasmid as well as the designed gene structure.

Additional file 2. The original, unprocessed and uncropped version of the Fig. 7 in manuscript.

Additional file 4. The original, unprocessed and uncropped version of the Fig. 9 in manuscript.

Additional file 3. The original, unprocessed and uncropped version of the Fig. 8 in manuscript.

Additional file 5. After generating the standard curves for each test by plotting the absorbance versus the concentration of each controls, concentrations of the active form of the hGH were obtained from the standard curve (all row data were reported in this supplementary data file).

Additional file 1: Figure S1. pChiM is a plasmid containing cell wall sorting motifs of B. subtilis (CWM), sortase cleavage motif (LPDTS) and the enzyme sortase (YhcS). This plasmid was used as a template for making other constructs prepared in this study. Figure S2. Schematic representation of SpA display constructs. Plasmids were constructed to express the SpA with or without the C-terminal cell wall motif (CWM) of YhcR, and with or without the sortase YhcS. a pSpA-YhcS is the main construct for SpA display on the B. subtilis cell wall surface, b the control construct named pNC1 lacks the sortase, c the control construct named pNC2 lacks both the CWM and sortase. SD Shine Dalgarno sequence; spa S. aureus protein A; CWM cell wall motif; YhcS sortase of B. subtilis. Figure S3. Diagrammatic representation of the constructs used in this study as control plasmids (pNC1 and pNC2) and main construct (pSpA-YhcS). Figure S4. The process of making constructs and their transformation. Table S1. Oligonucleotide primers sequences used in this study.