Search

Your search keyword '"Ahn, Chun-Seob"' showing total 32 results
Start Over Author "Ahn, Chun-Seob"
32 results on '"Ahn, Chun-Seob"'

15. Human Sparganosis in Korea

Author

Kim, Jeong-Geun, primary, Ahn, Chun-Seob, additional, Sohn, Woon-Mok, additional, Nawa, Yukifumi, additional, and Kong, Yoon, additional

Published
2018
Full Text
View/download PDF

28. Biochemical Characterization of Echinococcus multilocularisAntigen B3 Reveals Insight into Adaptation and Maintenance of Parasitic Homeostasis at the Host–Parasite Interface

Author

Ahn, Chun-Seob, Kim, Jeong-Geun, Han, Xiumin, Bae, Young-An, Park, Woo-Jae, Kang, Insug, Wang, Hu, and Kong, Yoon

Abstract

Alveolar echinococcosis (AE) caused by Echinococcus multilocularismetacestode is frequently associated with deleterious zoonotic helminthiasis. The growth patterns and morphological features of AE, such as invasion of the liver parenchyme and multiplication into multivesiculated masses, are similar to those of malignant tumors. AE has been increasingly detected in several regions of Europe, North America, Central Asia, and northwestern China. An isoform of E. multilocularisantigen B3 (EmAgB3) shows a specific immunoreactivity against patient sera of active-stage AE, suggesting that EmAgB3 might play important roles during adaptation of the parasite to hosts. However, expression patterns and biochemical properties of EmAgB3 remained elusive. The protein profile and nature of component proteins of E. multilocularishydatid fluid (EmHF) have never been addressed. In this study, we conducted proteome analysis of EmHF of AE cysts harvested from immunocompetent mice. We observed the molecular and biochemical properties of EmAgB3, including differential transcription patterns of paralogous genes, macromolecular protein status by self-assembly, distinct oligomeric states according to individual anatomical compartments of the worm, and hydrophobic ligand-binding protein activity. We also demonstrated tissue expression patterns of EmAgB3 transcript and protein. EmAgB3 might participate in immune response and recruitment of essential host lipids at the host–parasite interface. Our results might contribute to an in depth understanding of the biophysical and biological features of EmAgB3, thus providing insights into the design of novel targets to control AE.

Published
2017
Full Text
View/download PDF

30. An Echinococcus multilocularisAntigen B3 Proteoform That Shows Specific Antibody Responses to Active-Stage Alveolar Echinococcosis

Author

Ahn, Chun-Seob, Cai, Huixia, Kim, Jeong-Geun, Han, Xiumin, Ma, Xiao, Bae, Young-An, Yang, Hyun-Jong, Kang, Insug, Wang, Hu, and Kong, Yoon

Abstract

ABSTRACTAlveolar echinococcosis (AE), caused by the Echinococcus multilocularismetacestode, represents one of the most frequently fatal zoonoses. Early diagnosis significantly reduces morbidity and mortality associated with AE. Diagnosis of AE largely depends on a combination of imaging and serological tests due to its minimal clinical manifestations. Several antigens derived from the whole worm and protoscolex have been targeted for AE serodiagnosis, while the antigenic properties of E. multilocularishydatid fluid (EmHF) are unclear. We observed two AE-specific 6- and 8-kDa antigen proteoforms through an immunoproteome array of the EmHF. We identified these proteins as representing an E. multilocularisantigen B3 (EmAgB3) isoform, and the proteins were shown to be encoded by the same gene. We cloned the gene and expressed the recombinant EmAgB3 protein (rEmAgB3) in Escherichia coli. rEmAgB3 exhibited sensitivity of 90.9% (80/88 cases) and specificity of 98.5% (597/606 samples) by immunoblotting. The positive and negative predictive values were 89.9% and 98.6%, respectively. The protein did not show antibody responses to 33 AE sera collected during posttreatment follow-up monitoring. Mouse sera experimentally infected with AE protoscoleces began to demonstrate specific antibody responses to native and recombinant EmAgB3 6 months after infection. At that stage, fully mature metacestode vesicles that harbored the brood capsule, primary cell, and protoscolex were observed within an AE mass(es). The response declined along with worm degeneration. Our results demonstrate that the immune responses to this EmAgB3 isoform were highly correlated with worm viability accompanied with AE progression. rEmAgB3 is a promising biomarker for serological assessment of AE patients.

Published
2015
Full Text
View/download PDF

31. Aspergillus nidulans translationally controlled tumor protein has a role in the balance between asexual and sexual differentiation and normal hyphal branching.

Author

Oh, Young Taek, Ahn, Chun-Seob, Jeong, Yu Jeong, Kim, Jeong Geun, Ro, Hyeon-Su, Han, Kap-Hoon, Lee, Chang-Won, and Kim, Jae Won

Subjects
*ASPERGILLUS nidulans, *TUMOR proteins, *SEX differentiation (Embryology), *HYPHOMYCETES, *CONIDIA, *CYTOPLASM
Abstract

Translationally controlled tumor protein ( TCTP) is a highly conserved and ubiquitously expressed protein present in all eukaryotes. Cellular functions of TCTP include growth promoting, allergic response and responses to various cellular stresses, but the functions in filamentous fungi have not been reported. In this report, we characterized an Aspergillus nidulans TCTP ( Tcp A) with high similarity to TCTP. The level of tcpa m RNA was relatively high, both during vegetative growth stage and at early phases of development. Tcp A was found predominantly in the nucleus during germination and mycelial growth, and was localized in cytoplasm and nuclei of vesicles on stipes during conidia development. Deletion of tcpA resulted in abnormal hyphal branch formation during vegetative growth. The tcpA deletion inhibited sexual development, but enhanced asexual development via induction of brlA expression. These results imply that Tcp A is involved in normal hyphal branch establishment during vegetative growth and also has a role in the balance between asexual and sexual differentiation. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

32. Biochemical Characterization of Echinococcus multilocularis Antigen B3 Reveals Insight into Adaptation and Maintenance of Parasitic Homeostasis at the Host-Parasite Interface.

Author

Ahn CS, Kim JG, Han X, Bae YA, Park WJ, Kang I, Wang H, and Kong Y

Subjects
Adaptation, Physiological genetics, Adaptation, Physiological immunology, Animals, Cloning, Molecular, Disease Models, Animal, Echinococcosis, Echinococcosis, Hepatic genetics, Echinococcosis, Hepatic parasitology, Echinococcosis, Hepatic pathology, Echinococcus multilocularis growth & development, Echinococcus multilocularis pathogenicity, Escherichia coli genetics, Escherichia coli metabolism, Female, Gene Expression, Gene Expression Regulation, Helminth Proteins genetics, Homeostasis genetics, Homeostasis immunology, Humans, Lipoproteins genetics, Mice, Oleic Acid metabolism, Proteome genetics, Recombinant Proteins genetics, Recombinant Proteins immunology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Echinococcosis, Hepatic immunology, Echinococcus multilocularis immunology, Helminth Proteins immunology, Host-Parasite Interactions, Lipoproteins immunology, Proteome immunology
Abstract

Alveolar echinococcosis (AE) caused by Echinococcus multilocularis metacestode is frequently associated with deleterious zoonotic helminthiasis. The growth patterns and morphological features of AE, such as invasion of the liver parenchyme and multiplication into multivesiculated masses, are similar to those of malignant tumors. AE has been increasingly detected in several regions of Europe, North America, Central Asia, and northwestern China. An isoform of E. multilocularis antigen B3 (EmAgB3) shows a specific immunoreactivity against patient sera of active-stage AE, suggesting that EmAgB3 might play important roles during adaptation of the parasite to hosts. However, expression patterns and biochemical properties of EmAgB3 remained elusive. The protein profile and nature of component proteins of E. multilocularis hydatid fluid (EmHF) have never been addressed. In this study, we conducted proteome analysis of EmHF of AE cysts harvested from immunocompetent mice. We observed the molecular and biochemical properties of EmAgB3, including differential transcription patterns of paralogous genes, macromolecular protein status by self-assembly, distinct oligomeric states according to individual anatomical compartments of the worm, and hydrophobic ligand-binding protein activity. We also demonstrated tissue expression patterns of EmAgB3 transcript and protein. EmAgB3 might participate in immune response and recruitment of essential host lipids at the host-parasite interface. Our results might contribute to an in depth understanding of the biophysical and biological features of EmAgB3, thus providing insights into the design of novel targets to control AE.

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results