1. Individual bioaerosol particle discrimination by multi-photon excited fluorescence
- Author
-
Luigi Bonacina, Jean-Pierre Wolf, and Denis Kiselev
- Subjects
Materials science ,010504 meteorology & atmospheric sciences ,Nanotechnology ,ddc:500.2 ,Biosensing Techniques ,01 natural sciences ,Molecular physics ,Fluorescence ,010309 optics ,symbols.namesake ,Optics ,0103 physical sciences ,Laser-induced breakdown spectroscopy ,0105 earth and related environmental sciences ,Aerosols ,Air Pollutants ,Biological Products ,Spectrometry ,business.industry ,Air ,Air and pollutants analysis ,Atomic and Molecular Physics, and Optics ,Spectrometry, Fluorescence ,Coherent control ,Excited state ,Femtosecond ,symbols ,Particle ,Particulate Matter ,business ,Raman scattering ,Bioaerosol ,Environmental Monitoring - Abstract
Femtosecond laser induced multi-photon excited fluorescence (MPEF) from individual airborne particles is tested for the first time for discriminating bioaerosols. The fluorescence spectra, analysed in 32 channels, exhibit a composite character originating from simultaneous two-photon and three-photon excitation at 790 nm. Simulants of bacteria aggregates (clusters of dyed polystyrene microspheres) and different pollen particles (Ragweed, Pecan, Mulberry) are clearly discriminated by their MPEF spectra. This demonstration experiment opens the way to more sophisticated spectroscopic schemes like pump-probe and coherent control.
- Published
- 2011