1. Caveolin expression changes in the neurovascular unit after juvenile traumatic brain injury: Signs of blood–brain barrier healing?
- Author
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Badaut, J., Ajao, D.O., Sorensen, D.W., Fukuda, A.M., and Pellerin, L.
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CAVEOLINS , *BRAIN injuries , *BLOOD-brain barrier , *WOUND healing , *PEDIATRICS , *IMMUNOGLOBULIN G , *GENE expression - Abstract
Traumatic brain injury (TBI) is one of the major causes of death and disability in pediatrics, and results in a complex cascade of events including the disruption of the blood–brain barrier (BBB). A controlled-cortical impact on post-natal 17-day-old rats induced BBB disruption by IgG extravasation from 1 to 3 days after injury and returned to normal at day 7. In parallel, we characterized the expression of three caveolin isoforms, caveolin 1 (cav-1), caveolin 2 (cav-2) and caveolin 3 (cav-3). While cav-1 and cav-2 are expressed on endothelial cells, both cav-1 and cav-3 were found to be present on reactive astrocytes, in vivo and in vitro . Following TBI, cav-1 expression was increased in blood vessels at 1 and 7 days in the perilesional cortex. An increase of vascular cav-2 expression was observed 7 days after TBI. In contrast, astrocytic cav-3 expression decreased 3 and 7 days after TBI. Activation of endothelial nitric oxide synthase (eNOS) (via its phosphorylation) was detected 1 day after TBI and phospho-eNOS was detected both in association with blood vessels and with astrocytes. The molecular changes involving caveolins occurring in endothelial cells following juvenile-TBI might participate, independently of eNOS activation, to a mechanism of BBB repair while, they might subserve other undefined roles in astrocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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