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2. Effects of inhibition of myo-inositol transport on MDCK cells under hypertonic environment

Author

Kitamura, Hiroshi, Yamauchi, Atsushi, Nakanishi, Takeshi, Takamitsu, Yoshihiro, Sigiura, Toshihiro, Akagi, Akiko, Moriyama, Toshiki, Horio, Masaru, and Imai, Enyu

Subjects
Renal tubular transport -- Physiological aspects, Inositol -- Physiological aspects, Kidneys -- Physiological aspects, Dogs as laboratory animals -- Physiological aspects, Biological sciences
Abstract

The effects of 2-omicron, C-methylene-myo-inositol (MMI) on myo-inositol transport were analyzed in Madin-Darby canine kidney (MDCK) cells to determine the role of myo-inositol under hypertonic conditions. Analysis of myo-inositol transport in MDCK cells after the administration of MMI indicated the presence of inhibited myo-inositol transport due to the inactivation of the Na+-dependent component. Furthermore, MMI coupled with betaine increased the colony-forming efficiency of MDCK cells.

Published
1997

6. EST analysis of genes that are expressed in the neural complex of Ciona intestinalis adults.

Author

Takamura, Katsumi, Oka, Nobuo, Akagi, Akiko, Okamoto, Kenji, Okada, Taro, Fukuoka, Takuya, Hogaki, Ayumu, Naito, Dai, Oobayashi, Yuji, Satoh, Nori, Takamura, Katsumi, Oka, Nobuo, Akagi, Akiko, Okamoto, Kenji, Okada, Taro, Fukuoka, Takuya, Hogaki, Ayumu, Naito, Dai, Oobayashi, Yuji, and Satoh, Nori

Abstract

A subtractive cDNA library was made corresponding to mRNAs expressed in the neural complex relative to those expressed in the pharynx of adults of the ascidian Ciona intestinalis. Determination and comparison of expressed sequence tags (ESTs) of a set of 1,527 randomly selected clones demonstrated that they represent 832 independent sequences. Five hundred seventy-two of the clones contained amino-acid-encoding sequences. BLASTX analyses showed that 342 of the 572 clones were strong matches (P<10(-7)) to previously identified proteins, while the remaining 230 fell into the "no match" category. Among the clones matching previously identified proteins, about 80 clones represented proteins that are involved in the formation, maintenance of the structure, and function of the nervous system: 22 proteins are associated with signal transduction, five proteins are related to the synapse, 11 to transcription factors, nine to transporters, five to enzymes, and 13 to extracellular matrix and cytoskeletal components, and six to apoptosis. In addition, sequence information for genes associated with the immune system and for genes encoding proteins with interesting functions were obtained. These data provide cues for further studies on genes that are expressed in and function in the ascidian nervous system.

Published
2001

10. Transcriptional activation of a hybrid promoter composed of cytomegalovirus enhancer and β-actin/β-globin gene in glomerular epithelial cells in vivo

Author

Akagi, Yoshitaka, primary, Isaka, Yoshitaka, additional, Akagi, Akiko, additional, Ikawa, Masahito, additional, Takenaka, Masaru, additional, Moriyama, Toshiki, additional, Yamauchi, Atsushi, additional, Horio, Masaru, additional, Ueda, Naohiko, additional, Okabe, Masaru, additional, and Imai, Enyu, additional

Published
1997
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