272 results on '"Akira Iwase"'
Search Results
2. Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology
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Ayako Muraoka, Akira Yokoi, Kosuke Yoshida, Masami Kitagawa, Eri Asano-Inami, Mayuko Murakami, Bayasula, Natsuki Miyake, Natsuki Nakanishi, Tomoko Nakamura, Satoko Osuka, Akira Iwase, and Hiroaki Kajiyama
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Medicine - Abstract
Abstract Background Assisted reproductive technology accounts for an increasing proportion of infertility treatments, and assessments to predict clinical pregnancy outcomes are desired. Extracellular vesicles exist in follicular fluid, and small non coding RNAs in extracellular vesicles underline the possibility of reflecting pregnancy potential. Methods Follicular fluid samples are collected from 20 ovarian follicles of 15 infertile patients undergoing assisted reproductive technology. Extracellular vesicles are isolated by serial centrifugation and small RNA sequencing is performed to investigate the profiles of microRNAs and P-element-induced wimpy testis-interacting RNAs. Results Small extracellular vesicles with a size range of approximately 100 nm are successfully isolated, and the small non coding RNA profiles of pregnant samples (n = 8) are different from those of non-pregnant samples (n = 12). Fourteen dysregulated small non coding RNAs are selected to identify the independent candidates [mean read count >100, area under the curve >0.8]. Among them, we find that a specific combination of small non coding RNAs (miR-16-2-3p, miR-378a-3p, and miR-483-5p) can predict the pregnant samples more precisely using a receiver operating characteristics curves analysis (area under the curve: 0.96). Furthermore, even in the same patients, the three microRNAs are differentially expressed between pregnant and non-pregnant samples. Conclusions Our results demonstrate that small non coding RNAs derived from small extracellular vesicles in follicular fluid can be potential non-invasive biomarkers for predicting pregnancy, leading to their probable application in assisted reproductive technology. Further large-scale studies are required to validate the clinical usefulness of these small non coding RNAs.
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- 2024
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3. Single coronary artery diagnosed in the perinatal period: A case report
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Ayuko Tanaka, Daisuke Higeta, Syuhei Arai, Yohei Ishibashi, Takuji Kyoya, and Akira Iwase
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Cesarean section ,Perinatal care ,Pregnancy ,Single coronary artery ,Transthoracic echocardiography ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: Having a single coronary artery (SCA) is a rare congenital anomaly in which a single artery arises from the aorta. Although most cases of SCA are asymptomatic and incidental, its effects during the perinatal period remain unknown. Herein, we report a case of pregnant woman with suspected SCA, based on transthoracic echocardiography (TTE) findings. Case report: A 33-year-old multiparous woman presented with preterm premature rupture of the membrane at 29 weeks gestation. The patient’s preoperative electrocardiogram (ECG) showed slight ST changes. TTE showed dilated right coronary artery and hypoplastic left coronary artery. Cesarean section was performed at 30 weeks of gestation due to non-reassuring fetal status. Although poor oxygenation was observed postoperatively, the patient was managed appropriately. She was diagnosed with SCA based on coronary computed tomographic angiography findings one month after delivery. Conclusion: Pregnant women with SCA require careful perinatal care.
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- 2023
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4. Anti-Müllerian hormone beyond an ovarian reserve marker: the relationship with the physiology and pathology in the life-long follicle development
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Akira Iwase, Yuko Hasegawa, Yumiko Tsukui, Mio Kobayashi, Hikaru Hiraishi, Tomoko Nakazato, and Yoshikazu Kitahara
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anti-Müllerian hormone ,follicle cohort ,oocyte ,ovarian reserve ,puberty ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Anti-Müllerian hormone (AMH), an indirect indicator of the number of remaining follicles, is clinically used as a test for ovarian reserve. Typically, a decline suggests a decrease in the number of remaining follicles in relation to ovarian toxicity caused by interventions, which may implicate fertility. In contrast, serum AMH levels are elevated in patients with polycystic ovary syndrome. AMH is produced primarily in the granulosa cells of the preantral and small antral follicles. Thus it varies in association with folliculogenesis and the establishment and shrinking of the follicle cohort. Ovarian activity during the female half-life, from the embryonic period to menopause, is based on folliculogenesis and maintenance of the follicle cohort, which is influenced by developmental processes, life events, and interventions. AMH trends over a woman’s lifetime are associated with in vivo follicular cohort transitions that cannot be observed directly.
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- 2023
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5. Predictive factors for massive hemorrhage in women with retained products of conception: a prospective study
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Reina Sonehara, Tomoko Nakamura, Akira Iwase, Kazuki Nishida, Sachiko Takikawa, Mayuko Murakami, Sayako Yoshita, Ayako Muraoka, Natsuki Miyake, Natsuki Nakanishi, Satoko Osuka, Maki Goto, and Hiroaki Kajiyama
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Medicine ,Science - Abstract
Abstract Retained products of conception (RPOC) is a common cause of postpartum bleeding, which may be life-threatening; however, no evidence-based guidelines exist to assist in evaluating the risk of massive hemorrhage in women with RPOC. In this prospective study, we aimed to evaluate the predictive factors for massive hemorrhage in women with RPOC. The primary and secondary endpoints were to validate the usefulness of power Doppler color scoring (PDCS) in evaluating hypervascularity and to identify other predictive factors (such as maximum RPOC diameter and serum βhCG and Hb level at first visit), respectively. Among the 51 women with RPOC included in this study, 16 (31.5%) experienced massive hemorrhage during follow-up. None of the women with PDCS 1 or 2 (18) experienced massive hemorrhage, whereas 16 (48.5%) women with PDCS 3 or 4 (33) did. Multiple logistic regression analysis showed that the odds ratio [95% confidence interval] (P value) for PDCS, assisted reproductive technology (ART), and low serum hemoglobin (Hb) levels were 22.39 [2.25 − 3087.92] (P = 0.004), 5.72 [1.28 − 33.29] (P = 0.022), and 4.24 [0.97 − 22.99] (P = 0.056), respectively. Further, the decision tree method identified PDCS, ART, and low serum Hb levels as potential predictive factors for massive hemorrhage. This study identified PDCS as useful predictor of massive hemorrhage in women with RPOC. With additional inclusion of factors such as ART and low serum Hb levels, the risk of massive hemorrhage may be effectively evaluated, leading to better management of women of reproductive age.
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- 2022
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6. Serum leucine‐rich α2‐glycoprotein as a possible marker for inflammatory status in endometriosis
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Mio Kobayashi, Orie Nakamura, Yoshikazu Kitahara, Naoki Inoue, Yumiko Tsukui, Yuko Hasegawa, Hikaru Hiraishi, Atsushi Yabuki, Ayako Muraoka, Satoko Osuka, and Akira Iwase
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biomarker ,dienogest ,endometriosis ,inflammation ,LRG ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Reproduction ,QH471-489 - Abstract
Abstract Purpose This study aimed to investigate whether serum leucine‐rich α2‐glycoprotein (LRG) is a useful diagnostic biomarker for endometriosis, including the evaluation of treatment efficacy and exploration of LRG production in endometriotic lesions. Methods Forty‐three women with endometriomas were compared to 22 women with benign ovarian cysts and 30 women who underwent assisted reproduction as controls. Changes in serum LRG levels were assessed before and after surgery, and during dienogest treatment. LRG expression in endometriotic tissue samples was evaluated using immunoblotting. Results Serum LRG levels in the endometrioma group (80.0 ± 36.3 μg/mL) were significantly higher than those in the benign ovarian cyst (65.1 ± 27.0 μg/mL, p = 0.0265) and control (57.8 ± 22.3 μg/mL, p = 0.0028) groups. Serum LRG levels after endometrioma surgery were significantly lower than preoperative levels (p = 0.0484). Serum LRG levels consistently decreased during dienogest treatment. LRG expression levels were significantly higher in endometriotic tissues than in the normal endometrium. Conclusion Serum LRG, possibly derived from local and systemic origins, could be used as a potential biomarker for the diagnosis and treatment of endometriosis.
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- 2023
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7. Follicle development and its prediction in patients with primary ovarian insufficiency: Possible treatments and markers to maximize the ability to conceive with residual follicles
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Satoko Osuka, Yukiyo Kasahara, Shohei Iyoshi, Reina Sonehara, Natsuki Myake, Ayako Muraoka, Tomoko Nakamura, Akira Iwase, and Hiroaki Kajiyama
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anti‐Müllerian hormone ,enzyme‐linked immunosorbent assay ,hypogonadism ,premature ovarian failure ,primary ovarian insufficiency ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Reproduction ,QH471-489 - Abstract
Abstract Background Primary ovarian insufficiency (POI) is characterized by the development of hypergonadotropic hypogonadism before 40 years of age and leads to intractable infertility. Although in vitro fertilization and embryo transfer with donated eggs enables pregnancy, not a few patients desire pregnancy using their oocytes. However, follicular development is rare and unpredictable in patients with POI. Thus, there is a need for treatments that promote the development of residual follicles and methods to accurately predict infrequent ovulation. Methods This review discusses the effects of various treatments for obtaining eggs from POI patients. Furthermore, this study focused a potential marker for predicting follicular growth in patients with POI. Main Findings Different treatments such as hormone‐replacement therapy, dehydroepiandrosterone supplementation, platelet‐rich plasma injection, and in vitro activation have shown varying degrees of effectiveness in retrieving oocytes from patients with POI. To predict follicle development in the cycle, elevated serum estradiol and reduced follicle‐stimulating hormone (FSH) levels are important. However, these markers are not always reliable under continuous estradiol‐replacement therapy. As a novel marker for predicting follicle growth, serum anti‐Müllerian hormone (AMH) levels, measured using the picoAMH enzyme‐linked immunosorbent assay, were found to predict follicle growth in patients and the cycle. Conclusion This review highlights the challenges and available interventions for achieving pregnancy using a patient's oocytes in cases of POI. We believe that a combination of currently available treatments and prediction methods is the best strategy to enable patients with POI to conceive using their own eggs. Although AMH levels may predict follicle growth, further research is necessary to improve the chances of successful follicular development and conception in patients with POI.
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- 2023
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8. Impact of perioperative use of GnRH agonist or dienogest on ovarian reserve after cystectomy for endometriomas: a randomized controlled trial
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Ayako Muraoka, Satoko Osuka, Atsushi Yabuki, Bayasula, Masato Yoshihara, Hideaki Tanaka, Reina Sonehara, Natsuki Miyake, Mayuko Murakami, Sayako Yoshita, Natsuki Nakanishi, Tomoko Nakamura, Maki Goto, Akira Iwase, and Hiroaki Kajiyama
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Anti-Müllerian hormone ,Cystectomy ,Endometriomas ,Ovarian reserve ,Gynecology and obstetrics ,RG1-991 ,Reproduction ,QH471-489 - Abstract
Abstract Background Ovarian endometrioma is a common gynecological disease that is often treated with surgery or hormonal treatment. Ovarian cystectomy, a surgical procedure for ovarian endometrioma, can result in impaired ovarian reserve. Methods We conducted a randomized controlled trial to evaluate the efficacy of hormonal treatment [gonadotropin-releasing hormone agonist (GnRHa) or dienogest (DNG)] for preserving ovarian reserve after cystectomy for ovarian endometrioma. The primary endpoint was the level of serum Anti-Müllerian hormone (AMH) as a marker of ovarian reserve. Results Before and after laparoscopic surgery, 22 patients in the GnRHa group and 27 patients in the DNG group were administered hormonal treatment for a total of 4 months. After 1-year follow-up, >60% of the patients in the DNG group retained over 70% of their pretreatment AMH levels, whereas no patient in the GnRHa group retained their AMH levels after cystectomy (P < 0.01). Interleukin-6 (IL-6) is a key cytokine involved in inflammation. Compared with the GnRHa group, patients in the DNG group had lower IL-6 levels at the end of treatment. Conclusions Our data revealed that DNG is more effective than GnRHa in preserving ovarian reserve after cystectomy of ovarian endometrioma. This is achieved through the reduction of the inflammatory response during the perioperative period and other endometriosis-related inflammatory reactions. Trial registration The registration number of this trial is UMIN-CTR, UMIN000018569, registered 6 August 2015, https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000021492 , and Japan Registry of Clinical Trials, jRCTs041180140, registered 29 March 2019, https://jrct.niph.go.jp/en-latest-detail/jRCTs041180140 . This randomized controlled trial was conducted in accordance with the CONSORT guidelines.
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- 2021
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9. Malignancies Associated with Extraovarian Endometriosis: A Literature Review
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Naoki Inoue, Takashi Hirakawa, Junji Mitsushita, Yoshikazu Kitahara, and Akira Iwase
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clear cell carcinoma ,extraovarian endometriosis ,endometrioid carcinoma ,hormone replacement therapy ,infertility ,pelvic pain ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Endometriosis-associated ovarian malignancies have been well documented. Although these malignancies also occur as extraovarian lesions, little is known about them. Thus, this literature review aimed to further explore these rarely experienced tumors. A total of 257 published cases between April 1990 and April 2020 were found using PubMed, and 212 cases were included in the analysis considering Sampson’s criteria and the history of endometriosis. We classified these cases as follows: intestine, abdominal scar, vagina and vulva, peritoneum and deep endometriosis, urinary tract, uterine cervix, and others. Age of patients, history of endometriosis, types of past hormonal therapy, symptoms, histological types, and treatment were identified. The most common tumor site was the intestine. Endometrioid carcinoma was the dominant histological type. Contrary to the ovary, clear cell carcinoma was rare in extraovarian sites. On the other hand, clear cell carcinoma represented the largest number of abdominal scars. This difference may help us to understand the development of endometriosis-related malignancies. Hormonal treatment was mentioned in 67 cases and estrogen replacement therapy in 33 cases. Although risks of estrogen therapy are still controversial, the highly differentiated histological types and hormone-dependent characteristics of endometriosis-associated malignancy should be considered. Physicians should be careful about estrogen monotherapy after hysterectomy and long-term hormone replacement therapy in patients with a history of endometriosis.
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- 2021
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10. Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells
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Yukiyo Kasahara, Satoko Osuka, Nobuyoshi Takasaki, Bayasula, Yoshihiro Koya, Natsuki Nakanishi, Tomohiko Murase, Tomoko Nakamura, Maki Goto, Akira Iwase, and Hiroaki Kajiyama
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Patients with primary ovarian insufficiency (POI) often have a high prevalence of autoimmune disorders. To identify antigenic molecules associated with ovarian autoimmunity, we performed immunoprecipitation (IP) screening using serum from patients with POI and the established human granulosa cell line (HGrC1). POTE ankyrin domain family member E (POTEE) and POTE ankyrin domain family member F (POTEF), proteins specific to primates, were identified as candidate antigens. Using immunohistochemistry (IHC) with human ovarian tissue, POTEE or POTEF was weakly seen in the granulosa cells (GCs) of primordial follicles and primary follicles, and strongly in large antral follicles and luteal cells. Interestingly, no signals were detected in growing GCs in secondary, preantral, and small antral follicles. Thus, to explore the function of POTEE and POTEF in human folliculogenesis, we established HGrC1 cell lines with drug-inducible expression of POTEF. Expression of POTEF significantly suppressed cell proliferation in HGrC1 cells. Furthermore, chaperonin containing TCP-1 complex (CCT) components, which affect folding proteins required for cell proliferation, was bound to the actin domain of POTEF protein. Although CCT is normally localized only around the Golgi apparatus, TCP-1α, a component of CCT, co-migrated closer to the cell membrane when POTEF expression was induced. These data suggest that the interaction between POTEF and CCT components impairs the usual function of CCT during cell growth. In addition, over-accumulation of POTEF in HGrC1 cells leads to autophagic failure. It was recently reported that knockout of an autophagic gene in mice leads to a phenotype similar to human POI. These results suggested that a proper amount of POTEF is required for the maintenance of GCs in follicle pools, whereas POTEF overaccumulation might be involved in follicle atresia and the development of POI. We also showed the possibility that POTEF could be an antigen involved in ovarian autoimmunity.
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- 2021
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11. Pregnancy in hereditary sensory and autonomic neuropathy type V: A case report and literature review
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Daisuke Higeta, Akihito Morita, Risa Uehara, Maki Inoue, Takashi Kameda, and Akira Iwase
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Anesthesia ,Cesarean section ,Congenital insensitivity to pain ,HSAN ,Pregnancy ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: Hereditary sensory and autonomic neuropathies (HSANs) are a clinical heterogenous group of inherited neuropathies featuring prominent sensory and autonomic involvement. We report on the management of pregnancy and delivery in a woman with HSAN type V (HSAN-V) that is a rare inherited disease characterized by pain insensitivity, and partial anhidrosis. Case report: A 25-year-old woman with HSAN-V at six weeks of gestation was referred to our hospital. She decided to continue her pregnancy after the genetic counseling. A multidisciplinary team including her decided to undergo cesarean section due to her short stature and the risk of an emergency in normal delivery. She successfully gave birth at 38 weeks of gestation by cesarean section under general anesthesia following an uneventful pregnancy course. Conclusion: Cesarean section seems favorable to vaginal delivery in women with HSANs.
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- 2022
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12. Postpartum unscarred uterine rupture caused by placenta accreta: A case report and literature review
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Jun Okaniwa, Daisuke Higeta, Takashi Kameda, Yosuke Uchiyama, Maki Inoue, and Akira Iwase
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placenta accreta ,postpartum ,retained placenta ,unscarred uterine rupture ,uterotonics ,Medicine ,Medicine (General) ,R5-920 - Abstract
Key Clinical Message Our case and the literature review suggest that placenta accreta spectrum, with use of uterotonics and manual removal of placenta, could be risk factors for postpartum unscarred uterine rupture.
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- 2021
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13. Effect of hypothyroidism and thyroid autoimmunity on the ovarian reserve: A systematic review and meta‐analysis
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Yuko Hasegawa, Yoshikazu Kitahara, Satoko Osuka, Yumiko Tsukui, Mio Kobayashi, and Akira Iwase
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adolescent ,AMH ,hypothyroidism ,ovarian reserve ,thyroid autoimmunity ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Reproduction ,QH471-489 - Abstract
Abstract Background Evidence suggests that hypothyroidism and thyroid autoimmunity (TAI) are possibly associated with ovarian dysfunction. This meta‐analysis aimed to investigate whether hypothyroidism and/or TAI affect the ovarian reserve and evaluated using the anti‐Mullerian hormone (AMH). Methods PubMed, EMBASE, Web of Science, and Cochrane Controlled Trials Register databases from inception to October 2020 were searched to identify relevant studies. Studies comparing the AMH levels between the control and the affected groups were included in the data synthesis. The primary endpoint in the meta‐analysis was AMH levels compared with the controls. Main findings Nine trials were included in the analysis. The AMH levels were significantly lower in the adults with euthyroid TAI (mean difference −0.12, [95% CI: −0.18 to −0.06]). The AMH levels tended to be lower in subclinical hypothyroidism and overt hypothyroidism than in the control group, although the differences were not significant. The AMH levels were significantly higher in the euthyroid TAI group in the adolescents (mean difference 2.51, [95% CI 1.82 to 3.21]). Conclusion TAI and hypothyroidism may affect the ovarian reserve. The opposite effects on AMH levels depending on age suggest that TAI may be implicated in the depletion of follicles in adults following extensive activation of primordial follicles in adolescence.
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- 2022
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14. Special Issue 'Impact of Endometriosis on Women’s Health'
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Osamu Wada-Hiraike and Akira Iwase
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n/a ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Endometriosis is one of the most common diseases in women of reproductive age, and although there are many theories to explain this enigmatic disease, such as reflux theory, metastasis theory, and metaplasia theory, there is still no single theory that can wholly explain the pathogenesis of the disease, and it is considered a mysterious disease until now [...]
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- 2022
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15. Animal models of polycystic ovary syndrome: A review of hormone‐induced rodent models focused on hypothalamus‐pituitary‐ovary axis and neuropeptides
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Satoko Osuka, Natsuki Nakanishi, Tomohiko Murase, Tomoko Nakamura, Maki Goto, Akira Iwase, and Fumitaka Kikkawa
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androgen ,animal models ,hypothalamic‐pituitary‐gonadal axis ,kisspeptin ,polycystic ovary syndrome ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Reproduction ,QH471-489 - Abstract
Abstract Background Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women of reproductive age and a major cause of infertility; however, the pathophysiology of this syndrome is not fully understood. This can be addressed using appropriate animal models of PCOS. In this review, we describe rodent models of hormone‐induced PCOS that focus on the perturbation of the hypothalamic‐pituitary‐ovary (HPO) axis and abnormalities in neuropeptide levels. Methods Comparison of rodent models of hormone‐induced PCOS. Main findings The main method used to generate rodent models of PCOS was subcutaneous injection or implantation of androgens, estrogens, antiprogestin, or aromatase inhibitor. Androgens were administered to animals pre‐ or postnatally. Alterations in the levels of kisspeptin and related molecules have been reported in these models. Conclusion The most appropriate model for the research objective and hypothesis should be established. Dysregulation of the HPO axis followed by elevated serum luteinizing hormone levels, hyperandrogenism, and metabolic disturbance contribute to the complex etiology of PCOS. These phenotypes of the human disease are recapitulated in hormone‐induced PCOS models. Thus, evidence from animal models can help to clarify the pathophysiology of PCOS.
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- 2019
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16. Protective effects of mangafodipir against chemotherapy-induced ovarian damage in mice
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Ying Qin, Akira Iwase, Tomohiko Murase, Bayasula, Chiharu Ishida, Nao Kato, Tomoko Nakamura, Satoko Osuka, Sachiko Takikawa, Maki Goto, Tomomi Kotani, and Fumitaka Kikkawa
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Anticancer drug ,Follicle ,Mangafodipir ,Ovary ,Oxidative stress ,Gynecology and obstetrics ,RG1-991 ,Reproduction ,QH471-489 - Abstract
Abstract Background Given the seriousness of chemotherapy-induced ovarian injury in female cancer patients, the preservation of fertility, including through the use of cryopreservation technology and pharmaceuticals, requires investigation. Previous studies have shown that damage to the ovaries is related to oxidative stress caused by anticancer drugs. Therefore, superoxide dismutase (SOD) may represent a key factor in the pharmacological protection of the ovaries. The aim of our study was to identify the effects of mangafodipir, a manganese chelate and SOD-mimetic, on suppression of apoptosis in granulosa cells and primordial follicle activation induced by anticancer drugs. Methods Cell viability assays using methyltrichlorosilane solutions and immunoblotting for cleaved caspase-3 were performed in in vitro experiments with the simultaneous addition of mangafodipir to human non-luteinized granulosa cell line (HGrC) cultures treated with hydrogen peroxide (H2O2), cisplatin, or paclitaxel. Count and morphological analyses of follicles at each developing stage in the ovaries and immunohistochemistry for cleaved caspase-3, Ki67 and 4-hydroxynonenal, a marker for oxidative stress, were also performed using mangafodipir-injected 6-week-old female ICR mice treated with cisplatin or paclitaxel. Further, mangafodipir was injected into 6-week-old female BALB/c mice inoculated with ES-2 to analyze whether mangafodipir inhibits the anti-tumor effects of cisplatin or paclitaxel treatment. Results Mangafodipir attenuated apoptosis induced by H2O2 and anticancer drugs in vitro. Mangafodipir also decreased the expression of 4-hydroxynonenal and reduced cisplatin- and paclitaxel-induced apoptosis in granulosa cells in vivo. In addition, mangafodipir inhibited the loss of primordial follicles. Tumor xenograft studies in mice showed that mangafodipir did not affect anticancer drug antitumor effects. Conclusions Oxidative stress might be one of the mechanisms of cisplatin- and paclitaxel-induced the loss of primordial follicles. Mangafodipir can reduce cisplatin- and paclitaxel-induced apoptosis in granulosa cells and primordial follicle activation partially via its SOD activity. At the same time, mangafodipir might have other potential mechanisms to inhibit the activation of primordial follicles. Further, mangafodipir attenuated the ovarian damage caused by cisplatin and paclitaxel without affecting their antitumor activities. Mangafodipir, therefore, though its efficacy might be limited, may be a new option for the preservation of fertility during anticancer treatment.
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- 2018
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17. Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice
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Shotaro Hayashi, Tomoko Nakamura, Yashiro Motooka, Fumiya Ito, Li Jiang, Shinya Akatsuka, Akira Iwase, Hiroaki Kajiyama, Fumitaka Kikkawa, and Shinya Toyokuni
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Ovarian endometriosis ,Mouse model ,Iron ,Oxidative stress ,Follicle stimulating hormone receptor ,Infertility ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Ovarian endometriosis (OE) provides women of reproductive age with not only severe menstrual pain but also infertility and an increased risk for ovarian carcinogenesis. Whereas peritoneal endometriosis models have been developed with syngeneic implantation of minced uterine tissue and oncogenic K-ras allele with conditional Pten deletion within ovarian surface epithelium generated preneoplastic endometrial glandular morphology, followed by endometrioid adenocarcinoma, there has been no mouse model of OE similar to human counterparts, applicable to preclinical studies. Here we for the first time established a murine OE model that reveals infertility, and evaluated the involvement of iron catalyzed oxidative stress in the pathogenesis. Minced uterine tissue from female mice was implanted on ovarian surface of syngeneic mice after bursectomy to induce OE. Ectopic growth of endometrium was observed in association with ovary 4 weeks after implantation in 85.7% (12/14) of the operated mice with our protocol. Endometriotic lesions involved intestine, pancreas and peritoneal wall. Fibrosis around the ovary was prominent and increased time-dependently in the OE group. Iron accumulation was significantly increased in the OE group, leading to oxidative stress in each stage of the follicles as evaluated by 4-hydroxy-2-nonenal-modified proteins and 8-hydroxy-2′-deoxyguanosine. Expression of follicle stimulating hormone receptor in the follicles revealed a significant decrease during pre-antral, antral and pre-ovulatory phases in the OE group. Finally, the number of pups was significantly reduced in the OE group in comparison to the controls. This model affords an opportunity to evaluate agents or procedures to counteract ovarian endometriosis in the preclinical settings.
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- 2020
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18. Common Mechanisms of Developmental Reprogramming in Plants—Lessons From Regeneration, Symbiosis, and Parasitism
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Yasunori Ichihashi, Tsuneo Hakoyama, Akira Iwase, Ken Shirasu, Keiko Sugimoto, and Makoto Hayashi
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reprogrammed development ,lateral root development ,parasitism ,regeneration ,symbiosis ,Plant culture ,SB1-1110 - Abstract
Most plants are exquisitely sensitive to their environment and adapt by reprogramming post-embryonic development. The systematic understanding of molecular mechanisms regulating developmental reprogramming has been underexplored because abiotic and biotic stimuli that lead to reprogramming of post-embryonic development vary and the outcomes are highly species-specific. In this review, we discuss the diversity and similarities of developmental reprogramming processes by summarizing recent key findings in reprogrammed development: plant regeneration, nodule organogenesis in symbiosis, and haustorial formation in parasitism. We highlight the potentially shared molecular mechanisms across the different developmental programs, especially a core network module mediated by the AUXIN RESPONSIVE FACTOR (ARF) and the LATERAL ORGAN BOUNDARIES DOMAIN (LBD) family of transcription factors. This allows us to propose a new holistic concept that will provide insights into the nature of plant development, catalyzing the fusion of subdisciplines in plant developmental biology.
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- 2020
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19. Mutation Analysis of Radioresistant Early-Stage Cervical Cancer
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Tae Oike, Yoshihito Sekiguchi, Yuya Yoshimoto, Takahiro Oike, Ken Ando, Wenchao Gu, Yasushi Sasaki, Takashi Tokino, Akira Iwase, and Tatsuya Ohno
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cervical cancer ,early stage ,radiotherapy ,radioresistance ,mutation ,KRAS ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Radiotherapy is a definitive treatment for early-stage cervical cancer; however, a subset of this disease recurs locally, necessitating establishment of predictive biomarkers and treatment strategies. To address this issue, we performed gene panel-based sequencing of 18 stage IB cervical cancers treated with definitive radiotherapy, including two cases of local recurrence, followed by in vitro and in silico analyses. Simultaneous mutations in KRAS and SMAD4 (KRASmt/SMAD4mt) were detected only in a local recurrence case, indicating potential association of this mutation signature with radioresistance. In isogenic cell-based experiments, a combination of activating KRAS mutation and SMAD4 deficiency led to X-ray resistance, whereas either of these factors alone did not. Analysis of genomic data from 55,308 cancers showed a significant trend toward co-occurrence of mutations in KRAS and SMAD4. Gene Set Enrichment Analysis of the Cancer Cell Line Encyclopedia dataset suggested upregulation of the pathways involved in epithelial mesenchymal transition and inflammatory responses in KRASmt/SMAD4mt cancer cells. Notably, irradiation with therapeutic carbon ions led to robust killing of X-ray-resistant KRASmt/SMAD4mt cancer cells. These data indicate that the KRASmt/SMAD4mt signature is a potential predictor of radioresistance, and that carbon ion radiotherapy is a potential option to treat early-stage cervical cancers with the KRASmt/SMAD4mt signature.
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- 2021
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20. The Non-Cancer Specific Elevation of the Serum Squamous Cell Carcinoma Antigen during the Post-Radiotherapy Follow-Up of Cervical Cancer Patients
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Tae Oike, Takahiro Oike, Ken Ando, Akira Iwase, and Tatsuya Ohno
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squamous cell carcinoma antigen ,cervical cancer ,radiotherapy ,non-cancer specific elevation ,incidence ,renal dysfunction ,Medicine (General) ,R5-920 - Abstract
The elevation of the serum squamous cell carcinoma (SCC) antigen unrelated to disease progression occurs during the follow-up of patients with cervical cancer treated with radiotherapy. Although known empirically, the incidence and characteristics of this non-cancer specific elevation in SCC remain unclear. Here, we examined the post-treatment kinetics of SCC in 143 consecutive patients with squamous cell carcinoma of the cervix treated with definitive radiotherapy; in all patients, progression-free disease status was confirmed by periodic monitoring for at least 36 months (median, 61 months). We found that the 5-year cumulative incidence of post-treatment SCC elevation was unexpectedly high at 37.3% (59/143 patients), and that 59.3% (35/59) of event-positive patients experienced multiple events. The median peak SCC level for a given event was 2.0 ng/mL (interquartile range, 1.7–2.9 ng/mL). The multivariate analysis showed that renal dysfunction was associated significantly with a greater incidence of SCC elevation (p = 0.046). In addition, the 5-year cumulative incidence of SCC elevation was significantly greater in patients with renal dysfunction than in those without (54.8% vs. 32.9%, respectively; hazard ratio, 2.1 [95% confidence interval, 1.1–4.2]; p = 0.028). These data will be useful for monitoring cervical cancer patients treated with radiotherapy.
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- 2021
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21. Involvement of mesosalpinx in endometrioma is a possible risk factor for decrease of ovarian reserve after cystectomy: a retrospective cohort study
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Ai Saito, Akira Iwase, Tomoko Nakamura, Satoko Osuka, Bayasula, Tomohiko Murase, Nao Kato, Chiharu Ishida, Sachiko Takikawa, Maki Goto, and Fumitaka Kikkawa
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Anti-Müllerian hormone ,Cystectomy ,Endometriomas ,Mesosalpinx ,Ovarian reserve ,Gynecology and obstetrics ,RG1-991 ,Reproduction ,QH471-489 - Abstract
Abstract Background Serum anti-Müllerian hormone (AMH) concentration has been used to assess ovarian reserve in patients with endometriosis, especially when endometrioma surgery is involved. Previously, we reported that decreased serum AMH levels after cystectomy for endometriomas can recover to preoperative levels in some cases. In this present study, we assessed the sequential changes in serum AMH levels before and after cystectomy in terms of the state of the mesosalpinx prior to surgery. Methods The retrospective cohort study recruited 53 patients from a series of prospective studies conducted from 2009 to 2015. All patients underwent laparoscopic cystectomy for endometriomas. If either mesosalpinx was involved in the endometrioma or adnexal adhesion before cystectomy, the case was defined as ‘involved mesosalpinx’ (n = 14). If both mesosalpinx remained anatomically correct, the case was classified as ‘intact mesosalpinx’ (n = 39). Blood samples were obtained from the patients 2 weeks before surgery, and at 1 month and 1 year after surgery to assess serum AMH levels. Results The serum AMH levels (the involved group vs. the intact group) were 1.92 vs. 0.98 (P = 0.552) preoperatively, 0.59 vs. 1.99 (P = 0.049) at 1 month postoperatively, and 0.48 vs. 2.37 ng/mL (P = 0.007) at 1 year postoperatively. The involved mesosalpinx group showed a further decrease in serum AMH levels at 1 year postoperatively, while serum AMH levels in the intact mesosalpinx group tended to recover. Conclusion These results suggest that pre-existing mesosalpinx disturbance, in combination with adhesiolysis, may be involved in the medium- and long-term decrease in ovarian reserve after endometrioma surgery. A disturbance in ovarian blood supply via the mesosalpinx may underlie this. Trial registration UMIN-CTR UMIN000019369 . Retrospectively registered October 15, 2015.
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- 2016
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22. Choosing the optimal therapeutic strategy for placental polyps using power Doppler color scoring: Transarterial embolization followed by hysteroscopic resection or expectant management?
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Masahiko Mori, Akira Iwase, Satoko Osuka, Mika Kondo, Tomoko Nakamura, Tatsuo Nakahara, Maki Goto, and Fumitaka Kikkawa
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hysteroscopic resection ,placental polyps ,power Doppler ,transarterial embolization ,vascularity ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: To evaluate a protocol for selection of placental polyp management, including expectant management and hysteroscopic resection with or without transarterial embolization (TAE), using power Doppler color score (PDCS) as the vascularity parameter. Materials and Methods: This retrospective case–control study included 25 patients who were diagnosed with placental polyps. We evaluated the vascularity of placental polyps with PDCS measured by transvaginal ultrasonography as follows: PDCS 1, no blood flow; PDCS 2, minimal flow; PDCS 3, moderate flow; and PDCS 4, marked blood flow. We then selected expectant management or hysteroscopic resection with or without TAE. Results: Three of 17 patients with PDCS 1 or 2 underwent surgical intervention, and expectant management was successful in 14. Seven of eight patients with PDCS 3 or 4 underwent surgical intervention, while expectant management was successful in only one patient. Conclusion: PDCS is a simple examination for evaluating the vascularity of placental polyps. PDCS might be useful for selecting the optimal treatment for placental polyps, such as expectant management or surgical intervention, according to their vascularity.
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- 2016
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23. FGFR Signaling as a Candidate Therapeutic Target for Cancers Resistant to Carbon Ion Radiotherapy
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Narisa Dewi Maulany Darwis, Ankita Nachankar, Yasushi Sasaki, Toshiaki Matsui, Shin-ei Noda, Kazutoshi Murata, Tomoaki Tamaki, Ken Ando, Noriyuki Okonogi, Shintaro Shiba, Daisuke Irie, Takuya Kaminuma, Takuya Kumazawa, Mai Anakura, Souichi Yamashita, Takashi Hirakawa, Sangeeta Kakoti, Yuka Hirota, Takashi Tokino, Akira Iwase, Tatsuya Ohno, Atsushi Shibata, Takahiro Oike, and Takashi Nakano
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carbon ion radiotherapy ,uterine cervical cancer ,next-generation sequencer ,somatic mutations ,FGFR ,radiosensitization ,LY2874455 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Radiotherapy is an essential component of cancer therapy. Carbon ion radiotherapy (CIRT) promises to improve outcomes compared with standard of care in many cancers. Nevertheless, clinicians often observe in-field recurrence after CIRT. This indicates the presence of a subset of cancers that harbor intrinsic resistance to CIRT. Thus, the development of methods to identify and sensitize CIRT-resistant cancers is needed. To address this issue, we analyzed a unique donor-matched pair of clinical specimens: a treatment-naïve tumor, and the tumor that recurred locally after CIRT in the same patient. Exon sequencing of 409 cancer-related genes identified enrichment of somatic mutations in FGFR3 and FGFR4 in the recurrent tumor compared with the treatment-naïve tumor, indicating a pivotal role for FGFR signaling in cancer cell survival through CIRT. Inhibition of FGFR using the clinically available pan-FGFR inhibitor LY2874455 sensitized multiple cancer cell lines to carbon ions at 3 Gy (RBE: relative biological effectiveness), the daily dose prescribed to the patient. The sensitizer enhancement ratio was 1.66 ± 0.17, 1.27 ± 0.09, and 1.20 ± 0.18 in A549, H1299, and H1703 cells, respectively. Our data indicate the potential usefulness of the analytical pipeline employed in this pilot study to identify targetable mutations associated with resistance to CIRT, and of LY21874455 as a sensitizer for CIRT-resistant cancers. The results warrant validation in larger cohorts.
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- 2019
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24. Fertility-sparing operation for recurrence of uterine cervical perivascular epithelioid cell tumor
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Eiko Yamamoto, Kazuhiko Ino, Maiko Sakurai, Sachiko Takigawa, Akira Iwase, and Fumitaka Kikkawa
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Perivascular epithelioid cell tumors (PEComa) are mesenchymal tumors composed of histologically and immunohistochemically distinctive perivascular epithelial cells. Although the uterine corpus seems to be one of the most prevalent sites of involvement, PEComa of the uterine cervix are very rare. Only four cervical PEComa cases have been described, and were treated with hysterectomy and radiotherapy. We report a case of a 24-year-old nulligravida woman who presented with acute abdominal pain and was diagnosed with a rupture of an ovarian chocolate cyst. Subsequent surgery revealed that the tumor arose in the uterus, and the histological diagnosis was uterine PEComa with low potential malignancy. Recurrent PEComa in the uterine cervix were excised twice, and she remains disease free 12 months after the last operation. To the best of our knowledge, this is the first report of recurrent cervical PEComa with fertility-preserving surgery. Estimating the malignant potential and appropriate surgery are essential for young patients with uterine PEComa.
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- 2010
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25. National survey of abnormal uterine bleeding according to the <scp>FIGO</scp> classification in Japan
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Yoshikazu Kitahara, Osamu Hiraike, Hiroshi Ishikawa, Koji Kugu, Yasushi Takai, Osamu Yoshino, Masanori Ono, Ryo Maekawa, Ikuko Ota, and Akira Iwase
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Obstetrics and Gynecology - Abstract
To investigate the status of abnormal uterine bleeding (AUB) in Japan using the International Federation of Gynecology and Obstetrics (FIGO) classification (AUB system 1 and 2; PALM-COEIN) and to clarify the relationship between AUB symptoms and the diseases causing AUB.In a nationwide study, we enrolled first-time patients who visited target facilities during two consecutive weeks from December 1, 2019 to January 31, 2020. The FIGO classification was used to investigate patients with symptoms and causative diseases of AUB. Based on the proportion of patients in the nationwide study, 373 cases were included in the detailed survey. Survey items included symptoms of AUB according to AUB system 1, examination details, and causative diseases according to the PALM-COEIN classification.Within the study period, we encountered 61 740 first-time patients, of which 8081 (13.1%) were diagnosed with AUB. Among them, 39.9% had abnormal menstrual cycles and 56.9% had abnormal menstrual bleeding. In the survey, PALM had the highest percentage of AUB-L and COEIN had the highest percentage of AUB-O. Correspondence analysis showed that COEIN was strongly associated with abnormal menstrual cycles and PALM with abnormal menstrual bleeding.We conducted the first nationwide survey of AUB in Japan. The FIGO classification was a useful tool for the diagnosis of AUB, with a strong correlation between symptoms of AUB by AUB system 1 and the causative disease of AUB by PALM-COEIN. Conversely, a high percentage of AUB-N and AUB-E suggests that AUB system 1 and PALM-COEIN are ambiguous as diagnostic tools.
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- 2022
26. Preface to the special issue 'Stem cell reformation in plants'
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Akira, Iwase and Masaaki, Umeda
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Plant Science ,Preface ,Agronomy and Crop Science ,Biotechnology - Published
- 2022
27. 4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway
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Akira Iwase, Arika Takebayashi, Yuki Aoi, David S Favero, Shunsuke Watanabe, Mitsunori Seo, Hiroyuki Kasahara, and Keiko Sugimoto
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Original Paper ,fungi ,food and beverages ,Plant Science ,Agronomy and Crop Science ,Biotechnology - Abstract
4-Phenylbutyric acid (4PBA) is utilized as a drug to treat urea cycle disorders and is also being studied as a potential anticancer drug that acts via its histone deacetylase (HDAC) inhibitor activity. During a search to find small molecules that affect plant regeneration in Arabidopsis, we found that 4PBA treatment promotes this process by mimicking the effect of exogenous auxin. Specifically, plant tissue culture experiments revealed that a medium containing 4PBA enhances callus formation and subsequent shoot regeneration. Analyses with auxin-responsive or cytokinin-responsive marker lines demonstrated that 4PBA specifically enhances AUXIN RESPONSE FACTOR (ARF)-dependent auxin responses. Our western blot analyses showed that 4PBA treatment does not enhance histone acetylation in Arabidopsis, in contrast to butyric acid and trichostatin A, other chemicals often used as HDAC inhibitors, suggesting this mechanism of action does not explain the observed effect of 4PBA on regeneration. Finally, mass spectroscopic analysis and genetic approaches uncovered that 4PBA in Arabidopsis plants is converted to phenylacetic acid (PAA), a known natural auxin, in a manner independent of peroxisomal IBR3-related β-oxidation. This study demonstrates that 4PBA application promotes regeneration in explants via its auxin activity and has potential applications to not only plant tissue culture engineering but also research on the plant β-oxidation pathway.
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- 2022
28. Tamoxifen Activates Dormant Primordial Follicles in Mouse Ovaries
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Wei Wei, Kouji Komatsu, Satoko Osuka, Tomohiko Murase, Bayasula Bayasula, Natsuki Nakanishi, Tomoko Nakamura, Maki Goto, Akira Iwase, Satoru Masubuchi, and Hiroaki Kajiyama
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Obstetrics and Gynecology - Abstract
Our previous study found that 17β-estradiol (E2) suppresses primordial follicle activation and growth in cultured mouse ovaries. In this study, we administered tamoxifen, an estrogen receptor antagonist, into the abdominal cavity of mice to clarify the relationship between primordial follicle activation and the physiological concentration of E2 in mouse ovaries. The results showed that tamoxifen promoted primordial follicle activation. Administration of tamoxifen promoted degradation of the extracellular matrix surrounding primordial follicles in the ovaries. Furthermore, tamoxifen decreased the expression of stefin A, an inhibitor of cathepsins that digest some proteins and extracellular matrix, in the ovaries. Mechanical stress produced by the extracellular matrix reportedly suppresses the activation of primordial follicles. The collective results show that tamoxifen can promote primordial follicle activation through the degradation of the extracellular matrix surrounding primordial follicles. Our results indicate that E2 suppresses primordial follicle activation in vivo and that tamoxifen may be useful as a therapeutic agent against infertility. Graphical abstract
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- 2022
29. A case of laparoscopic total hysterectomy for uterine endometrial cancer in a patient with severe obesity and a giant thyroid tumor
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Azusa Kobayashi, Koshiro Nakao, Hisano Takakuwa, Amane Shuto, Anri Azuma, Tae Oike, Sadatomo Ikeda, Takashi Hirakawa, and Akira Iwase
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General Earth and Planetary Sciences ,General Environmental Science - Published
- 2022
30. How do plants reprogramme the fate of differentiated cells?
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Hatsune Morinaka, Yuki Sakamoto, Akira Iwase, and Keiko Sugimoto
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Plant Science - Published
- 2023
31. Decreased lamin A and B1 expression results in nuclear enlargement in serous ovarian carcinoma, whereas lamin A-expressing tumor cells metastasize to lymph nodes
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Miduki Ouchi, Sayaka Kobayashi, Yoshimi Nishijima, Naoki Inoue, Hayato Ikota, Akira Iwase, Hideaki Yokoo, and Masanao Saio
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Cell Biology ,Pathology and Forensic Medicine - Published
- 2023
32. A cell wall–localized cytokinin/purine riboside nucleosidase is involved in apoplastic cytokinin metabolism in Oryza sativa.
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Mikiko Kojima, Nobue Makita, Kazuki Miyata, Mika Yoshino, Akira Iwase, Miwa Ohashi, Alicia Surjana, Toru Kudo, Noriko Takeda-Kamiya, Kiminori Toyooka, Akio Miyao, Hirohiko Hirochika, Tsuyu Ando, Ayahiko Shomura, Masahiro Yano, Toshio Yamamoto, Tokunori Hobo, and Hitoshi Sakakibara
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RICE ,LOCUS (Genetics) ,RICE farmers ,GRAIN yields ,METABOLISM ,PLANT growth ,NATURAL products - Abstract
In the final step of cytokinin biosynthesis, the main pathway is the elimination of a ribose-phosphate moiety from the cytokinin nucleotide precursor by phosphoribohydrolase, an enzyme encoded by a gene named LONELY GUY (LOG). This reaction accounts for most of the cytokinin supply needed for regulating plant growth and development. In contrast, the LOG-independent pathway, in which dephosphorylation and deribosylation sequentially occur, is also thought to play a role in cytokinin biosynthesis, but the gene entity and physiological contribution have been elusive. In this study, we profiled the phytohormone content of chromosome segment substitution lines of Oryza sativa and searched for genes affecting the endogenous levels of cytokinin ribosides by quantitative trait loci analysis. Our approach identified a gene encoding an enzyme that catalyzes the deribosylation of cytokinin nucleoside precursors and other purine nucleosides. The cytokinin/purine riboside nucleosidase 1 (CPN1) we identified is a cell wall–localized protein. Loss-of-function mutations (cpn1) were created by inserting a Tos17-retrotransposon that altered the cytokinin composition in seedling shoots and leaf apoplastic fluid. The cpn1 mutation also abolished cytokinin riboside nucleosidase activity in leaf extracts and attenuated the trans-zeatin riboside-responsive expression of cytokinin marker genes. Grain yield of the mutants declined due to altered panicle morphology under field-grown conditions. These results suggest that the cell wall–localized LOG-independent cytokinin activating pathway catalyzed by CPN1 plays a role in cytokinin control of rice growth. Our finding broadens our spatial perspective of the cytokinin metabolic system. [ABSTRACT FROM AUTHOR]
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- 2023
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33. Molecular Mechanisms of Plant Regeneration from Differentiated Cells: Approaches from Historical Tissue Culture Systems
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Hatsune Morinaka, Duncan Coleman, Keiko Sugimoto, and Akira Iwase
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Physiology ,Cell Biology ,Plant Science ,General Medicine - Abstract
Plants can exert remarkable capacity for cell reprogramming even from differentiated cells. This ability allows plants to regenerate tissues/organs and even individuals in nature and in vitro. In recent decades, Arabidopsis research has uncovered molecular mechanisms of plant regeneration; however, our understanding of how plant cells retain both differentiated status and developmental plasticity is still obscure. In this review, we first provide a brief outlook of the representative modes of plant regeneration and key factors revealed by Arabidopsis research. We then re-examine historical tissue culture systems that enable us to investigate the molecular details of cell reprogramming in differentiated cells and discuss the different approaches, specifically highlighting our recent progress in shoot regeneration from the epidermal cell of Torenia fournieri.
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- 2022
34. A case of Abdominal Wall Endometriosis at the Caesarean Section Scar
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Ken Sirabe, Yumi Mori, Mio Kobayashi, Yuki Hirai, Yukie Yamatsu, Takaya Makiguchi, Hideharu Nakamura, Akira Iwase, Koki Shoda, Daichi Aoki, and Satoshi Yokoo
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Abdominal wall ,medicine.medical_specialty ,medicine.anatomical_structure ,business.industry ,medicine.medical_treatment ,Endometriosis ,Medicine ,Caesarean section ,General Medicine ,business ,medicine.disease ,Surgery - Published
- 2021
35. Malignancies Associated with Extraovarian Endometriosis: A Literature Review
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Akira Iwase, Junji Mitsushita, Naoki Inoue, Takashi Hirakawa, and Yoshikazu Kitahara
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extraovarian endometriosis ,medicine.medical_specialty ,clear cell carcinoma ,Hysterectomy ,business.industry ,medicine.medical_treatment ,Endometriosis ,Hormone replacement therapy (menopause) ,pelvic pain ,medicine.disease ,Malignancy ,RC648-665 ,Dermatology ,Diseases of the endocrine glands. Clinical endocrinology ,hormone replacement therapy ,Clear cell carcinoma ,medicine ,Carcinoma ,Hormonal therapy ,Sarcoma ,business ,infertility ,endometrioid carcinoma - Abstract
Endometriosis-associated ovarian malignancies have been well documented. Although these malignancies also occur as extraovarian lesions, little is known about them. Thus, this literature review aimed to further explore these rarely experienced tumors. A total of 257 published cases between April 1990 and April 2020 were found using PubMed, and 212 cases were included in the analysis considering Sampson’s criteria and the history of endometriosis. We classified these cases as follows: intestine, abdominal scar, vagina and vulva, peritoneum and deep endometriosis, urinary tract, uterine cervix, and others. Age of patients, history of endometriosis, types of past hormonal therapy, symptoms, histological types, and treatment were identified. The most common tumor site was the intestine. Endometrioid carcinoma was the dominant histological type. Contrary to the ovary, clear cell carcinoma was rare in extraovarian sites. On the other hand, clear cell carcinoma represented the largest number of abdominal scars. This difference may help us to understand the development of endometriosis-related malignancies. Hormonal treatment was mentioned in 67 cases and estrogen replacement therapy in 33 cases. Although risks of estrogen therapy are still controversial, the highly differentiated histological types and hormone-dependent characteristics of endometriosis-associated malignancy should be considered. Physicians should be careful about estrogen monotherapy after hysterectomy and long-term hormone replacement therapy in patients with a history of endometriosis.
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- 2021
36. Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells
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Tomohiko Murase, Hiroaki Kajiyama, Yoshihiro Koya, Tomoko Nakamura, Satoko Osuka, Natsuki Nakanishi, Yukiyo Kasahara, Akira Iwase, Bayasula, Maki Goto, and Nobuyoshi Takasaki
- Subjects
0301 basic medicine ,Cancer Research ,endocrine system ,Reproductive disorders ,Granulosa cell ,Immunology ,030209 endocrinology & metabolism ,Biology ,Article ,Cell membrane ,Cell growth ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,medicine ,Ankyrin ,Actin ,RC254-282 ,chemistry.chemical_classification ,QH573-671 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cell Biology ,Antral follicle ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Cell culture ,Folliculogenesis ,Cytology - Abstract
Patients with primary ovarian insufficiency (POI) often have a high prevalence of autoimmune disorders. To identify antigenic molecules associated with ovarian autoimmunity, we performed immunoprecipitation (IP) screening using serum from patients with POI and the established human granulosa cell line (HGrC1). POTE ankyrin domain family member E (POTEE) and POTE ankyrin domain family member F (POTEF), proteins specific to primates, were identified as candidate antigens. Using immunohistochemistry (IHC) with human ovarian tissue, POTEE or POTEF was weakly seen in the granulosa cells (GCs) of primordial follicles and primary follicles, and strongly in large antral follicles and luteal cells. Interestingly, no signals were detected in growing GCs in secondary, preantral, and small antral follicles. Thus, to explore the function of POTEE and POTEF in human folliculogenesis, we established HGrC1 cell lines with drug-inducible expression of POTEF. Expression of POTEF significantly suppressed cell proliferation in HGrC1 cells. Furthermore, chaperonin containing TCP-1 complex (CCT) components, which affect folding proteins required for cell proliferation, was bound to the actin domain of POTEF protein. Although CCT is normally localized only around the Golgi apparatus, TCP-1α, a component of CCT, co-migrated closer to the cell membrane when POTEF expression was induced. These data suggest that the interaction between POTEF and CCT components impairs the usual function of CCT during cell growth. In addition, over-accumulation of POTEF in HGrC1 cells leads to autophagic failure. It was recently reported that knockout of an autophagic gene in mice leads to a phenotype similar to human POI. These results suggested that a proper amount of POTEF is required for the maintenance of GCs in follicle pools, whereas POTEF overaccumulation might be involved in follicle atresia and the development of POI. We also showed the possibility that POTEF could be an antigen involved in ovarian autoimmunity.
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- 2021
37. Anti-Müllerian hormone levels in the diagnosis of adolescent polycystic ovarian syndrome: a systematic review and meta-analysis
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Yumiko Tsukui, Yoshikazu Kitahara, Yuko Hasegawa, Mio Kobayashi, Satoko Osuka, and Akira Iwase
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Anti-Mullerian Hormone ,Endocrinology ,Adolescent ,ROC Curve ,Endocrinology, Diabetes and Metabolism ,Infertility ,Peptide Hormones ,Humans ,Female ,Hyperandrogenism ,Polycystic Ovary Syndrome - Abstract
Polycystic ovary syndrome (PCOS) is an endocrine disorder that causes menstrual cycle irregularities and infertility. PCOS is diagnosed based on hyperandrogenism, polycystic ovarian morphology (PCOM), and an-/oligo-ovulation. Upregulation of anti-Müllerian hormone (AMH) in the serum of women with PCOS may be another suitable alternative diagnostic criterion for PCOM. However, previous meta-analyses have reported conflicting results due to the age-dependent decline in serum AMH levels. Therefore, we performed a meta-analysis to evaluate the threshold of AMH for the diagnosis of PCOS in adolescents and women in their early twenties. Fifteen trials were included in this meta-analysis. PCOS is diagnosed with either Rotterdam criteria, NIH, or AE-PCOS. AMH levels were significantly higher in adolescents with PCOS (weighted mean difference, 3.05; 95% confidence interval: 2.09-4.01) than in the control group. The cutoff values of AMH for the diagnosis of adolescent PCOS were 6.1, 6.26, 7.03, 7.11, 7.2, and 7.25 ng/mL in the studies that reported the usefulness of AMH levels. The summary receiver operating characteristic analysis of the diagnostic accuracy demonstrated that the specificity and sensitivity were 81% and 66.3%, respectively. Our meta-analysis demonstrates that AMH may be a useful diagnostic test for adolescent PCOS and, based on the previous studies included in the meta-analysis, its cutoff value was estimated to be 6-7 ng/mL.
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- 2022
38. Significance of the association between early embryonic development and endocytosis
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Akihito Morita, Yuhkoh Satouh, Ken Sato, and Akira Iwase
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Mice ,Pregnancy ,Oocytes ,Animals ,Embryonic Development ,Membrane Proteins ,Female ,General Medicine ,Aneuploidy ,Molecular Biology ,Endocytosis ,Pathology and Forensic Medicine - Abstract
Fertilization triggers a process called maternal-to-zygotic transition, in which the oocyte undergoes oocyte-to-embryo transition, leading to massive intracellular remodeling toward early embryogenesis. This transition requires the degradation of oocyte-derived components; however, the significance and mechanism of degradation of cell surface components remain unknown. In this review, we focused on the dynamics of plasma membrane proteins and investigated the relationship between embryonic development and endocytosis. Our survey of the extant literature on the topic led to the conclusion that clathrin-mediated endocytosis is essential for the progression of early embryogenesis and selective degradation of oocyte-derived plasma membrane proteins in mouse embryos, as reported by studies analyzing maternal cellular surface proteins, including a glycine transporter, GlyT1a. Evaluation of such endocytic activity in individual embryos may allow the selection of embryos with higher viability in assisted reproductive technologies, and it is important to select viable embryos to increase the rates of successful pregnancy and live birth. Although the early embryonic developmental abnormalities are mainly accompanied with chromosomal aneuploidy, other causes and mechanisms remain unclear. This review summarizes molecular biological approaches to early embryonic developmental abnormalities and their future prospects.
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- 2022
39. Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes
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Natsuki Nakanishi, Satoko Osuka, Tomohiro Kono, Hisato Kobayashi, Shinya Ikeda, Bayasula Bayasula, Reina Sonehara, Mayuko Murakami, Sayako Yoshita, Natsuki Miyake, Ayako Muraoka, Yukiyo Kasahara, Tomohiko Murase, Tomoko Nakamura, Maki Goto, Akira Iwase, and Hiroaki Kajiyama
- Subjects
Obstetrics and Gynecology - Abstract
Polycystic ovary syndrome (PCOS), a common endocrine disorder, is associated with impaired oocyte development, leading to infertility. However, the pathogenesis of PCOS has not been completely elucidated. This study aimed to determine the differentially expressed genes (DEGs) and epigenetic changes in the oocytes from a PCOS mouse model to identify the etiological factors. RNA-sequencing analysis revealed that 90 DEGs were upregulated and 27 DEGs were downregulated in mice with PCOS compared with control mice. DNA methylation analysis revealed 30 hypomethylated and 10 hypermethylated regions in the PCOS group. However, the DNA methylation status did not correlate with differential gene expression. The pathway enrichment analysis revealed that five DEGs (Rps21, Rpl36, Rpl36a, Rpl37a, and Rpl22l1) were enriched in ribosome-related pathways in the oocytes of mice with PCOS, and the immunohistochemical analysis revealed significantly upregulated expression levels of Rps21 and Rpl36. These results suggest that differential gene expression in the oocytes of mice in PCOS is related to impaired folliculogenesis. These findings improve our understanding of PCOS pathogenesis.
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- 2022
40. Effect of salpingectomy on ovarian reserve: A systematic review and meta-analysis
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Mio Kobayashi, Yoshikazu Kitahara, Yuko Hasegawa, Yumiko Tsukui, Hikaru Hiraishi, and Akira Iwase
- Subjects
Anti-Mullerian Hormone ,Salpingectomy ,Cross-Sectional Studies ,Obstetrics and Gynecology ,Humans ,Female ,Ovarian Reserve ,Retrospective Studies - Abstract
To determine the effect of salpingectomy on ovarian reserve.PubMed, EMBASE, Web of Science, Dynamed plus, and Cochrane Controlled Trials Register databases were searched from their inception to December 2020 to identify relevant studies, including cross-sectional studies, retrospective studies, and randomized controlled trials. Studies that compared anti-Müllerian hormone (AMH) levels and/or antral follicle count (AFC) between the control and salpingectomy groups or before and after surgery were included.Twenty-one articles were included in the systematic review. Meta-analyses were performed on 16 studies in which data were presented as mean ± SD values. A meta-analysis comparing AMH levels before and after surgery in the same patients showed no significant decrease in all cases, irrespective of whether it was unilateral or bilateral salpingectomy. There was no significant decrease in the AFC in the meta-analysis comparing levels before and after bilateral salpingectomy, either. In contrast, in the case-controlled study the salpingectomy group had significantly lower levels of AMH in all meta-analyses of unilateral and bilateral surgery (mean difference: -0.31, 95% confidence interval [CI]: -0.55, -0.07), only unilateral cases (mean difference: -0.28, 95% CI: -0.50, -0.06), and only bilateral cases (mean difference: -0.71, 95% CI: -1.19, -0.23). The salpingectomy group that included unilateral and bilateral cases had significantly lower AFC compared with no-surgery controls (mean difference: -1.31, 95% CI: -2.13, -0.48).Although not conclusive, it does appear that patients who underwent salpingectomy (either unilateral or bilateral) have a decreased ovarian reserve.
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- 2022
41. Postpartum unscarred uterine rupture caused by placenta accreta: A case report and literature review
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Daisuke Higeta, Maki Inoue, Jun Okaniwa, Akira Iwase, Takashi Kameda, and Yosuke Uchiyama
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medicine.medical_specialty ,Placenta accreta ,medicine.medical_treatment ,Uterus ,lcsh:Medicine ,Case Report ,placenta accreta ,Case Reports ,030204 cardiovascular system & hematology ,unscarred uterine rupture ,03 medical and health sciences ,0302 clinical medicine ,Retained placenta ,Laparotomy ,Placenta ,medicine ,postpartum ,Risk factor ,reproductive and urinary physiology ,lcsh:R5-920 ,Obstetrics ,business.industry ,lcsh:R ,retained placenta ,General Medicine ,medicine.disease ,Uterine rupture ,medicine.anatomical_structure ,Shock (circulatory) ,030220 oncology & carcinogenesis ,embryonic structures ,medicine.symptom ,business ,lcsh:Medicine (General) ,uterotonics - Abstract
Key Clinical Message Our case and the literature review suggest that placenta accreta spectrum, with use of uterotonics and manual removal of placenta, could be risk factors for postpartum unscarred uterine rupture., Our case and the literature review suggest that placenta accreta spectrum, with use of uterotonics and manual removal of placenta, could be risk factors for postpartum unscarred uterine rupture.
- Published
- 2021
42. High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues
- Author
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Phanu T. Serivichyaswat, Kai Bartusch, Martina Leso, Constance Musseau, Akira Iwase, Yu Chen, Keiko Sugimoto, Marcel Quint, and Charles W. Melnyk
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Hot Temperature ,Indoleacetic Acids ,Arabidopsis Proteins ,fungi ,Arabidopsis ,Botany ,food and beverages ,Biological Transport ,Plants, Genetically Modified ,Hypocotyl ,Mixed Function Oxygenases ,Plant Leaves ,Cytochrome P-450 Enzyme System ,Solanum lycopersicum ,Gene Expression Regulation, Plant ,Basic Helix-Loop-Helix Transcription Factors ,Regeneration ,Plant Biotechnology ,Cotyledon ,Molecular Biology ,Signal Transduction ,Developmental Biology - Abstract
Cellular regeneration in response to wounding is fundamental to maintain tissue integrity. Various internal factors including hormones and transcription factors mediate healing, but little is known about the role of external factors. To understand how the environment affects regeneration, we investigated the effects of temperature upon the horticulturally relevant process of plant grafting. We found that elevated temperatures accelerated vascular regeneration in Arabidopsis thaliana and tomato grafts. Leaves were crucial for this effect, as blocking auxin transport or mutating PHYTOCHROME INTERACTING FACTOR 4 (PIF4) or YUCCA2/5/8/9 in the cotyledons abolished the temperature enhancement. However, these perturbations did not affect grafting at ambient temperatures, and temperature enhancement of callus formation and tissue adhesion did not require PIF4, suggesting leaf-derived auxin specifically enhanced vascular regeneration in response to elevated temperatures. We also found that elevated temperatures accelerated the formation of inter-plant vascular connections between the parasitic plant Phtheirospermum japonicum and host Arabidopsis, and this effect required shoot-derived auxin from the parasite. Taken together, our results identify a pathway whereby local temperature perception mediates long distance auxin signaling to modify regeneration, grafting and parasitism. This article has an associated ‘The people behind the papers’ interview.
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- 2022
- Full Text
- View/download PDF
43. Exposure to Information and Seat Belt Use among Pregnant Women
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Akira Iwase, Shota Ogawa, Masahiro Itoh, Hiromitsu Shinozaki, Kunihiko Hayashi, and Masamichi Hoshino
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business.industry ,Cross-sectional study ,law ,Environmental health ,Seat belt ,Medicine ,Health education ,General Medicine ,business ,Automotive safety ,law.invention - Published
- 2020
44. Involvement of Transcription Factor 21 in the Pathogenesis of Fibrosis in Endometriosis
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Yukiyo Kasahara, Bayasula, Maki Goto, Nobuyoshi Takasaki, Takashi Nagai, Natsuki Nakanishi, Tomohiko Murase, Fumitaka Kikkawa, Umida Ganieva, Ayako Muraoka, Akira Iwase, Satoko Osuka, Tomoko Nakamura, and Shotaro Hayashi
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0301 basic medicine ,Stromal cell ,Endometriosis ,Periostin ,Pathology and Forensic Medicine ,Extracellular matrix ,Endometrium ,03 medical and health sciences ,0302 clinical medicine ,Stroma ,Fibrosis ,Basic Helix-Loop-Helix Transcription Factors ,medicine ,Humans ,Cells, Cultured ,Cell Proliferation ,030219 obstetrics & reproductive medicine ,business.industry ,Transfection ,medicine.disease ,030104 developmental biology ,Case-Control Studies ,Cancer research ,Ovarian Endometriosis ,Cytokines ,Female ,Stromal Cells ,business ,Cell Adhesion Molecules ,Biomarkers - Abstract
Repeated tissue injury and repair and fibrosis play a pivotal role in endometriosis. Fibrotic tissue consists of extracellular matrix proteins, regulated by transcriptional factors promoting cell proliferation and survival. Periostin is one of the putative key extracellular matrix proteins. This study aimed to determine whether transcription factor 21 (TCF21) is involved in the development of endometriosis as an upstream regulatory gene of periostin. Formalin-fixed, paraffin-embedded tissue samples [normal endometrium of women without endometriosis; eutopic endometrium of women with endometriosis; ovarian endometriosis (OE); and deep infiltrating endometriosis (DIE)] and respective cells were analyzed. Basal, transiently stimulated, and knocked down periostin and TCF21 concentrations in stromal cells of women with or without endometriosis were examined. Periostin and TCF21 expressions were undetected in normal endometrium of women without endometriosis, weakly positive in eutopic endometrium of women with endometriosis, moderately positive in OE, and strongly positive in DIE. Type 2 helper T-cell cytokines (IL-4, IL-13, and transforming growth factor-β1) increased the mRNA expression of periostin and TCF21. These cytokines, periostin, and TCF21 colocalized in the stroma of OE and DIE. siRNA against human TCF21 gene suppressed periostin expression. Transfection of TCF21 plasmid vector into stromal cells of women without endometriosis, which originally expressed neither periostin nor TCF21, resulted in TCF21 and periostin expression. TCF21 and periostin are involved in the regulation of fibrosis in endometriosis. TCF21 may be a promising therapeutic target and biomarker in endometriosis.
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- 2020
45. Sedentism, pottery and inland fishing in Late Glacial Japan: a reassessment of the Maedakochi site
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Hiroyuki Sato, Yuka Sasaki, Takeshi Yamazaki, Naoichiro Ichida, Akira Iwase, Yasuko Kuronuma, Dai Kunikita, Noriyoshi Oda, and Kazuki Morisaki
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010506 paleontology ,Archeology ,geography.geographical_feature_category ,060102 archaeology ,General Arts and Humanities ,Sedentism ,Fishing ,06 humanities and the arts ,01 natural sciences ,Archaeology ,Geography ,Archipelago ,Period (geology) ,0601 history and archaeology ,East Asia ,Glacial period ,Pottery ,0105 earth and related environmental sciences ,Chronology - Abstract
The Palaeolithic–Neolithic transition in East Asia is characterised by the transformation of mobile hunter-gatherer groups into sedentary communities. The existence of ‘ice-age’ pottery in the Japanese archipelago, however, is inconsistent with claims that directly link climatic warming with sedentism and the development of ceramics. Here, the authors reconsider the chronology and palaeoenvironment of the Maedakochi site in Tokyo. New AMS dating and environmental data suggest that intensified inland fishing in cold environments, immediately prior to the Late Glacial warm period, created conditions conducive to sedentism and the development of subsistence-related pottery.
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- 2019
46. Histone acetylation orchestrates wound-induced transcriptional activation and cellular reprogramming in Arabidopsis
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Motoaki Seki, Yuki Sakamoto, Bart Rymen, Momoko Ikeuchi, David S. Favero, Keiko Sugimoto, Tetsuji Kakutani, Ayako Kawamura, Alice Lambolez, Soichi Inagaki, Arika Takebayashi, François Roudier, Akira Iwase, Takamasa Suzuki, Kaori Sako, RIKEN Center for Sustainable Resource Science [Yokohama] (RIKEN CSRS), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), University of Tokyo, National Institut Genetic, Graduate University for Advanced Studies [Hayama] (SOKENDAI), Japan Science and Technology Agency, Kindai University, Chubu University, RIKEN Cluster, Yokohama City University (YCU), Univ Tokyo, National Institut of Genetic, Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), RIKEN 201701100428Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)15H05961Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science17H03704PHC SAKURA grant 36164XFPICS grant 7625University of Tokyo Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science, École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and RIKEN Center for Sustainable Resource Science (CSRS)
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0106 biological sciences ,Transcriptional Activation ,Plant molecular biology ,Callus formation ,Arabidopsis ,Medicine (miscellaneous) ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Article ,Epigenesis, Genetic ,Histones ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Gene expression ,Histone post-translational modifications ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,méthylation ,lcsh:QH301-705.5 ,030304 developmental biology ,Plant Diseases ,0303 health sciences ,biology ,integumentary system ,Arabidopsis Proteins ,Acetylation ,biology.organism_classification ,Cellular Reprogramming ,Plants, Genetically Modified ,acétylation ,Chromatin ,Cell biology ,Histone Code ,Histone ,lcsh:Biology (General) ,biology.protein ,Wounding ,H3K4me3 ,General Agricultural and Biological Sciences ,Reprogramming ,010606 plant biology & botany ,Plant regeneration ,expression des gènes - Abstract
Plant somatic cells reprogram and regenerate new tissues or organs when they are severely damaged. These physiological processes are associated with dynamic transcriptional responses but how chromatin-based regulation contributes to wound-induced gene expression changes and subsequent cellular reprogramming remains unknown. In this study we investigate the temporal dynamics of the histone modifications H3K9/14ac, H3K27ac, H3K4me3, H3K27me3, and H3K36me3, and analyze their correlation with gene expression at early time points after wounding. We show that a majority of the few thousand genes rapidly induced by wounding are marked with H3K9/14ac and H3K27ac before and/or shortly after wounding, and these include key wound-inducible reprogramming genes such as WIND1, ERF113/RAP2.6 L and LBD16. Our data further demonstrate that inhibition of GNAT-MYST-mediated histone acetylation strongly blocks wound-induced transcriptional activation as well as callus formation at wound sites. This study thus uncovered a key epigenetic mechanism that underlies wound-induced cellular reprogramming in plants., Bart Rymen et al. looked at the correlation between histone modifications and gene expression at early time points after wounding. They show that histone acetylation rapidly marks a number of genes, including wound-inducible reprogramming genes.
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- 2019
47. Association between FSH, E1, and E2 levels in urine and serum in premenopausal and postmenopausal women
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Kunihiko Hayashi, Kazue Nagai, Yoshikazu Kitahara, Yuki Ideno, Toshiyuki Yasui, Akira Iwase, Yoko Onizuka, and Junko Nakajima-Shimada
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Adult ,030213 general clinical medicine ,medicine.medical_specialty ,Follicle-stimulating hormone (FSH) ,Estrone ,Clinical Biochemistry ,Urine ,030204 cardiovascular system & hematology ,Estradiol (E2) ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Chemiluminescent immunoassay ,Internal medicine ,medicine ,Humans ,Aged ,Postmenopausal women ,Estradiol ,medicine.diagnostic_test ,business.industry ,Radioimmunoassay ,General Medicine ,Middle Aged ,Serum samples ,Postmenopause ,Endocrinology ,Premenopause ,chemistry ,Estrone (E1) ,Immunoassay ,Measurement of sex hormones in urine ,Female ,Follicle Stimulating Hormone ,Menopausal status ,business ,hormones, hormone substitutes, and hormone antagonists ,Hormone - Abstract
Objective We aimed to establish correlations for the levels of follicle-stimulating hormone (FSH), estrone (E1) and estradiol (E2) between urine and serum in premenopausal and postmenopausal women using immunoassays. Methods In this study of 92 women (61 postmenopausal, 31 premenopausal), both urine and blood specimens were collected on the same day and stored at 4 °C for analysis by chemiluminescent immunoassay, radioimmunoassay and/or electrochemiluminescent immunoassay. Results There were correlations in the levels of FSH, E1 and E2 between urine and serum in both postmenopausal (r = 0.96 for FSH, r = 0.91 for E1, r = 0.80 for E2) and premenopausal (r = 0.98 for FSH, r = 0.92 for E1, r = 0.90 for E2) women. It is indicated that the correlations were stronger in the premenopausal group compared with the postmenopausal group, especially for FSH. Conclusion The levels of FSH, E1 and E2 in urine correlated with those in the serum in premenopausal and postmenopausal women. Urine samples could be used instead of serum samples to measure hormone levels, which would reduce the difficulty of conducting large survey studies.
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- 2019
48. Warm Temperature Promotes Shoot Regeneration in Arabidopsis thaliana
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Alice Lambolez, Ayako Kawamura, Tatsuya Takahashi, Bart Rymen, Akira Iwase, David S Favero, Momoko Ikeuchi, Takamasa Suzuki, Sandra Cortijo, Katja E Jaeger, Philip A Wigge, and Keiko Sugimoto
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Histones ,Physiology ,Arabidopsis Proteins ,Gene Expression Regulation, Plant ,fungi ,Arabidopsis ,Temperature ,food and beverages ,Cell Biology ,Plant Science ,General Medicine ,Hormones ,Plant Shoots - Abstract
Many plants are able to regenerate upon cutting, and this process can be enhanced in vitro by incubating explants on hormone-supplemented media. While such protocols have been used for decades, little is known about the molecular details of how incubation conditions influence their efficiency. In this study, we find that warm temperature promotes both callus formation and shoot regeneration in Arabidopsis thaliana. We show that such an increase in shoot regenerative capacity at higher temperatures correlates with the enhanced expression of several regeneration-associated genes, such as CUP-SHAPED COTYLEDON 1 (CUC1) encoding a transcription factor involved in shoot meristem formation and YUCCAs (YUCs) encoding auxin biosynthesis enzymes. ChIP-sequencing analyses further reveal that histone variant H2A.Z is enriched on these loci at 17°C, while its occupancy is reduced by an increase in ambient temperature to 27°C. Moreover, we provide genetic evidence to demonstrate that H2A.Z acts as a repressor of de novo shoot organogenesis since H2A.Z-depleted mutants display enhanced shoot regeneration. This study thus uncovers a new chromatin-based mechanism that influences hormone-induced regeneration and additionally highlights incubation temperature as a key parameter for optimizing in vitro tissue culture.
- Published
- 2021
49. Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Findings of Differential Gene Expression Analysis of Oocytes
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Natsuki Nakanishi, Satoko Osuka, Tomohiro Kono, Hisato Kobayashi, Shinya Ikeda, Bayasula Bayasula, Tomomi Seki, Atsushi Yabuki, Hideaki Tanaka, Reina Sonehara, Mayuko Murakami, Sayako Yoshita, Natsuki Miyake, Ayako Muraoka, Yukiyo Kasahara, Tomohiko Murase, Tomoko Nakamura, Maki Goto, Akira Iwase, and Hiroaki Kajiyama
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endocrine system diseases - Abstract
Background: Polycystic ovary syndrome (PCOS), a common endocrinal disorder, is associated with impaired oocyte development, which leads to infertility. However, the pathogenesis of PCOS has not been completely elucidated. Limited studies have analyzed the pathological characteristics of oocytes in PCOS. This study aimed to analyze the differentially expressed genes (DEGs) and epigenetic changes in the oocytes of the PCOS mouse model to identify the etiological factors.Methods: C57BL/6J female mice were subcutaneously injected with vehicle or 5α-dihydrotestosterone (250 µg/day) on days 16–18 of pregnancy. Female offspring were used as the control or PCOS group. The oocytes were collected from mice aged 7–9 weeks. The DEGs between the control and PCOS groups were analyzed using RNA sequencing (RNA-Seq). Additionally, the DNA methylation status was analyzed using the post-bisulfite adaptor tagging method. The ovarian tissue sections were stained with hematoxylin and eosin to examine the morphological changes. The proteins, Rps21 and Rpl36, were measured using immunostaining.Results: Compared with the control group, the PCOS group exhibited impaired estrous cycle and polycystic ovary-like morphology. RNA-Seq analysis revealed that 90 DEGs were upregulated and 27 DEGs were downregulated in the PCOS mouse model. DNA methylation analysis revealed 30 hypomethylated and 10 hypermethylated regions in the PCOS group. However, the DNA methylation status was not correlated with differential gene expression. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that five DEGs (Rps21, Rpl36, Rpl36a, Rpl37a, and Rpl22l1) were enriched in ribosome-related pathways. The immunohistochemical analysis revealed that the expression levels of Rps21 and Rpl36 were significantly upregulated in the PCOS mouse model.Conclusions: These results suggest that differential gene expression in the oocytes of the PCOS mouse model is related to impaired folliculogenesis. These findings improved our understanding of the pathogenesis of PCOS.
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- 2021
50. Effect of hypothyroidism and thyroid autoimmunity on the ovarian reserve: A systematic review and meta‐analysis
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Yuko Hasegawa, Yoshikazu Kitahara, Satoko Osuka, Yumiko Tsukui, Mio Kobayashi, and Akira Iwase
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ovarian reserve ,endocrine system ,endocrine system diseases ,Reproductive Medicine ,adolescent ,thyroid autoimmunity ,AMH ,Reviews ,Review ,hypothyroidism ,Cell Biology ,human activities ,hormones, hormone substitutes, and hormone antagonists - Abstract
Background Evidence suggests that hypothyroidism and thyroid autoimmunity (TAI) are possibly associated with ovarian dysfunction. This meta‐analysis aimed to investigate whether hypothyroidism and/or TAI affect the ovarian reserve and evaluated using the anti‐Mullerian hormone (AMH). Methods PubMed, EMBASE, Web of Science, and Cochrane Controlled Trials Register databases from inception to October 2020 were searched to identify relevant studies. Studies comparing the AMH levels between the control and the affected groups were included in the data synthesis. The primary endpoint in the meta‐analysis was AMH levels compared with the controls. Main findings Nine trials were included in the analysis. The AMH levels were significantly lower in the adults with euthyroid TAI (mean difference −0.12, [95% CI: −0.18 to −0.06]). The AMH levels tended to be lower in subclinical hypothyroidism and overt hypothyroidism than in the control group, although the differences were not significant. The AMH levels were significantly higher in the euthyroid TAI group in the adolescents (mean difference 2.51, [95% CI 1.82 to 3.21]). Conclusion TAI and hypothyroidism may affect the ovarian reserve. The opposite effects on AMH levels depending on age suggest that TAI may be implicated in the depletion of follicles in adults following extensive activation of primordial follicles in adolescence.
- Published
- 2021
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