16 results on '"Al-Adham I"'
Search Results
2. Cell membrane effects of some common biocides
- Author
-
AL-Adham, I S I, Dinning, A J, Eastwood, I M, Austin, P, and Collier, P J
- Published
- 1998
- Full Text
- View/download PDF
3. Microemulsions are highly effective anti-biofilm agents
- Author
-
Al-Adham, I. S.I., Al-Hmoud, N. D., Khalil, E., Kierans, M., and Collier, P. J.
- Published
- 2003
4. Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1
- Author
-
Winder, C. L., Al-Adham, I. S.I., Malek, S. M.A. Abdel, Buultjens, T. E.J., Horrocks, A. J., and Collier, P. J.
- Published
- 2000
5. Microemulsions are membrane-active, antimicrobial, self-preserving systems
- Author
-
Al-Adham, I. S.I., Khalil, E., Al-Hmoud, N. D., Kierans, M., and Collier, P. J.
- Published
- 2000
6. A novel assay for the distribution of pyrithione biocides in bacterial cells
- Author
-
Dinning, A. J., AL-Adham, I. S.I., Austin, P., and Collier, P. J.
- Published
- 1998
7. The Formulation and Evaluation of Deep Eutectic Vehicles for the Topical Delivery of Azelaic Acid for Acne Treatment.
- Author
-
Luhaibi DK, Ali HHM, Al-Ani I, Shalan N, Al-Akayleh F, Al-Remawi M, Nasereddin J, Qinna NA, Al-Adham I, and Khanfar M
- Subjects
- Animals, Rabbits, Dicarboxylic Acids chemistry, Skin, Pharmaceutical Preparations, Choline chemistry, Solvents chemistry, Dermatologic Agents therapeutic use, Acne Vulgaris drug therapy
- Abstract
The current work was aimed at the development of a topical drug delivery system for azelaic acid (AzA) for acne treatment. The systems tested for this purpose were deep eutectic systems (DESs) prepared from choline chloride (CC), malonic acid (MA), and PEG 400. Three CC to MA and eight different MA: CC: PEG400 ratios were tested. The physical appearance of the tested formulations ranged from solid and liquid to semisolid. Only those that showed liquid formulations of suitable viscosity were considered for further investigations. A eutectic mixture made from MA: CC: PEG400 1:1:6 (MCP 116) showed the best characteristics in terms of viscosity, contact angle, spreadability, partition coefficient, and in vitro diffusion. Moreover, the MCP116 showed close rheological properties to the commercially available market lead acne treatment product (Skinorin
® ). In addition, the formula showed synergistic antibacterial activity between the MA moiety of the DES and the AzA. In vitro diffusion studies using polyamide membranes demonstrated superior diffusion of MCP116 over the pure drug and the commercial product. No signs of skin irritation and edema were observed when MCP116 was applied to rabbit skin. Additionally, the MCP116 was found to be, physically and chemically, highly stable at 4, 25, and 40 °C for a one-month stability study.- Published
- 2023
- Full Text
- View/download PDF
8. Therapeutic Drug Monitoring of Vancomycin in Jordanian Patients. Development of Physiologically-Based Pharmacokinetic (PBPK) Model and Validation of Class II Drugs of Salivary Excretion Classification System (SECS).
- Author
-
Arabyat M, Abdul-Sattar A, Al-Fararjah F, Al-Ghazawi A, Rabayah A, Al-Hasassnah R, Mohmmad W, Al-Adham I, Hamadi S, and Idkaidek N
- Subjects
- Albumins pharmacology, Anti-Bacterial Agents pharmacology, Area Under Curve, Bayes Theorem, Chromatography, Liquid, Creatinine, Drug Monitoring methods, Humans, Jordan, Microbial Sensitivity Tests, Salivary Elimination, Tandem Mass Spectrometry, Methicillin-Resistant Staphylococcus aureus, Vancomycin pharmacokinetics, Vancomycin therapeutic use
- Abstract
Vancomycin is a commonly used antibiotic for multi-drug resistant gram-positive infections treatment, especially methicillin-resistant Staphylococcus aureus (MRSA). Despite that, it has wide individual pharmacokinetic variability and nephrotoxic effect. Vancomycin trough concentrations for 57 Jordanian patients were measured in plasma and saliva through immunoassay and liquid chromatography-mass spectrometry (LC-MS/MS), respectively. Plasma levels were within accepted normal range, with exception of 6 patients who showed trough levels of more than 20 μg/ml and vancomycin was discontinued. Bayesian dose-optimizing software was used for patient-specific pharmacokinetics prediction and AUC/MIC calculation. Physiological-based pharmacokinetic (PBPK) vancomycin model was built and validated through GastroPlus™ 9.8 using in-house plasma data. A weak correlation coefficient of 0.2478 (P=0.1049) was found between plasma and saliva concentrations. The suggested normal saliva trough range of vancomycin is 0.01906 to 0.028589 (μg/ml). Analysis of variance showed significant statistical effects of creatinine clearance and albumin concentration on dose-normalized Cmin plasma and saliva levels respectively, which is in agreement with PBPKmodeling. It can be concluded that saliva is not a suitable matrix for TDM of vancomycin. Trough levels in plasma matrix should always be monitored for the safety of patients., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
9. A review of the antimicrobial activity of thermodynamically stable microemulsions.
- Author
-
Al-Adham ISI, Jaber N, Al-Remawi M, Al-Akayleh F, Al-Kaissi E, Ali Agha ASA, Fitzsimmons LB, and Collier PJ
- Subjects
- Anti-Bacterial Agents, Emulsions pharmacology, Water, Anti-Infective Agents pharmacology, Surface-Active Agents pharmacology
- Abstract
Microemulsions are thermodynamically stable, transparent, isotropic mixtures of oil, water and surfactant (and sometimes a co-surfactant), which have shown potential for widespread application in disinfection and self-preservation. This is thought to be due to an innate antimicrobial effect. It is suggested that the antimicrobial nature of microemulsions is the result of a combination of their inherent kinetic energy and their containing surfactants, which are known to aid the disruption of bacterial membranes. This review examines the contemporary evidence in support of this theory., (© 2021 The Society for Applied Microbiology.)
- Published
- 2022
- Full Text
- View/download PDF
10. Antimicrobial potential of natural deep eutectic solvents.
- Author
-
Al-Akayleh F, Khalid RM, Hawash D, Al-Kaissi E, Al-Adham ISI, Al-Muhtaseb N, Jaber N, Al-Remawi M, and Collier PJ
- Subjects
- Anti-Bacterial Agents chemistry, Anti-Bacterial Agents pharmacology, Candida albicans, Escherichia coli, Menthol pharmacology, Solvents, Staphylococcus aureus, Water pharmacology, Anti-Infective Agents chemistry, Anti-Infective Agents pharmacology, Deep Eutectic Solvents
- Abstract
Natural deep eutectic solvents (NADES) are a new class of green solvents, which can solubilize natural and synthetic chemicals of low water solubility. NADES are mixtures of two or three compounds of hydrogen bond acceptors and hydrogen bond donors. Many NADES' components are of natural origin and therefore, NADES are presumed to be nontoxic and often exhibit antimicrobial activity. This work aimed to investigate the potential antimicrobial effect of menthol, capric acid and Solutol™, and their associated eutectic system on two Gram-positive bacteria (Staphylococcus aureus ATCC 6538 and Bacillus subtilis ATCC 6633), two Gram-negative bacteria (Escherichia coli ATCC 8739 and Pseudomonas aeruginosa ATCC 9027) and one fungus (the yeast Candida albicans ATCC 10231). The results obtained showed a stronger antimicrobial effect for the NADES when compared to their individual components and that they exhibit a promising antimicrobial activity against S. aureus and C. albicans and good activity against P. aeruginosa. NADES exhibited no observable antimicrobial activity against spore-forming B. subtilis., (© 2022 The Society for Applied Microbiology.)
- Published
- 2022
- Full Text
- View/download PDF
11. Saliva versus Plasma Therapeutic Drug Monitoring of Gentamicin in Jordanian Preterm Infants. Development of a Physiologically-Based Pharmacokinetic (PBPK) Model and Validation of Class II Drugs of Salivary Excretion Classification System.
- Author
-
Idkaidek N, Hamadi S, Bani-Domi R, Al-Adham I, Alsmadi M, Awaysheh F, Aqrabawi H, Al-Ghazawi A, and Rabayah A
- Subjects
- Bacterial Infections blood, Chromatography, High Pressure Liquid instrumentation, Chromatography, High Pressure Liquid methods, Dose-Response Relationship, Drug, Drug Dosage Calculations, Drug Monitoring instrumentation, Female, Gentamicins administration & dosage, Gentamicins adverse effects, Gentamicins isolation & purification, Humans, Infant, Low Birth Weight, Infant, Newborn, Infant, Premature, Jordan, Limit of Detection, Male, Ototoxicity blood, Ototoxicity etiology, Plasma chemistry, Saliva chemistry, Salivary Elimination physiology, Tandem Mass Spectrometry instrumentation, Tandem Mass Spectrometry methods, Bacterial Infections drug therapy, Drug Monitoring methods, Gentamicins pharmacokinetics, Models, Biological, Ototoxicity prevention & control
- Abstract
Gentamicin has proven to be a very successful treatment for bacterial infection, but it also can cause adverse effects, especially ototoxicity, which is irreversible. Therapeutic drug monitoring (TDM) in saliva is a more convenient non-invasive alternative compared to plasma. A physiologically-based pharmacokinetic (PBPK) model of gentamicin was built and validated using previously-published plasma and saliva data. The validated model was then used to predict experimentally-observed plasma and saliva gentamicin TDM data in Jordanian pediatric preterm infant patients measured using sensitive LCMS/MS method. A correlation was established between plasma and saliva exposures. The developed PBPK model predicted previously reported gentamicin levels in plasma, saliva and those observed in the current study. A good correlation was found between plasma and saliva exposures. The PBPK model predicted that gentamicin in saliva is 5-7 times that in plasma, which is in agreement with observed results. Saliva can be used as an alternative for TDM of gentamicin in preterm infant patients. Exposure to gentamicin in plasma and saliva can reliably be predicted using the developed PBPK model in patients., Competing Interests: The authors declare no conflict of interest., (Thieme. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
12. Saliva Versus Plasma Bioequivalence of Azithromycin in Humans: Validation of Class I Drugs of the Salivary Excretion Classification System.
- Author
-
Idkaidek N, Arafat T, Hamadi H, Hamadi S, and Al-Adham I
- Subjects
- Administration, Oral, Azithromycin administration & dosage, Chromatography, Liquid, Cross-Over Studies, Healthy Volunteers, Humans, Mass Spectrometry, Pilot Projects, Azithromycin blood, Azithromycin pharmacokinetics, Saliva metabolism, Salivary Elimination
- Abstract
Aim: The aim of this study was to compare human pharmacokinetics and bioequivalence metrics in saliva versus plasma for azithromycin as a model class I drug of the Salivary Excretion Classification System (SECS)., Methods: A pilot, open-label, two-way crossover bioequivalence study was done, and involved a single 500-mg oral dose of azithromycin given to eight healthy subjects under fasting conditions, followed by a 3-week washout period. Blood and unstimulated saliva samples were collected over 72 h and deep frozen until analysis by a validated liquid chromatography with mass spectroscopy method. The pharmacokinetic parameters and bioequivalence metrics of azithromycin were calculated by non-compartment analysis using WinNonlin V5.2. Descriptive statistics and dimensional analysis of the pharmacokinetic parameters of azithromycin were performed using Microsoft Excel. PK-Sim V5.6 was used to estimate the effective intestinal permeability of azithromycin., Results and Discussion: No statistical differences were shown in area under the concentration curves to 72 h (AUC
0-72 ), maximum measured concentration (Cmax ) and time to maximum concentration (Tmax ) between test and reference azithromycin products (P > 0.05) in the saliva matrix and in the plasma matrix. Due to the high intra-subject variability and low sample size of this pilot study, the 90% confidence intervals of AUC0-72 and Cmax did not fall within the acceptance range (80-125%). However, saliva levels were higher than that of plasma, with a longer salivary Tmax . The mean saliva/plasma concentration of test and reference were 2.29 and 2.33, respectively. The mean ± standard deviation ratios of saliva/plasma of AUC0-72 , Cmax and Tmax for test were 2.65 ± 1.59, 1.51 ± 0.49 and 1.85 ± 1.4, while for the reference product they were 3.37 ± 2.20, 1.57 ± 0.77 and 2.6 ± 1.27, respectively. A good correlation of R = 0.87 between plasma and saliva concentrations for both test and reference products was also observed. Azithromycin is considered a class I drug based on the SECS, since it has a high permeability and high fraction unbound, and saliva sampling could be used as an alternative to plasma sampling to characterize its pharmacokinetics and bioequivalence in humans when adequate sample size is used.- Published
- 2017
- Full Text
- View/download PDF
13. Proanthocyanidins from Ginkgo biloba leaf extract and their radical scavenging activity.
- Author
-
Qa'dan F, Mansoor K, AL-Adham I, Schmidt M, and Nahrstedt A
- Subjects
- Plant Leaves chemistry, Proanthocyanidins chemistry, Proanthocyanidins pharmacology, Free Radical Scavengers isolation & purification, Ginkgo biloba chemistry, Plant Extracts analysis, Proanthocyanidins isolation & purification
- Abstract
Context: Ginkgo biloba L (Ginkgoaceae) is a traditional herbal medicinal plant for the treatment of mild to moderate cognitive disorders, tinnitus, and dementia. These uses may be correlated with the presence of radical scavenging compounds., Objective: The chemical composition and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the flavan-3-ols and proanthocyanidins from G. biloba were studied., Material and Methods: The compounds have been isolated using column chromatography on Sephadex LH-20 and MCI gel and the structures were determined on the basis of 1D- and 2D-NMR (HSQC, HMBC) experiments of their peracetylated derivatives, MALDI-TOF-MS and by acid-catalyzed degradation with phloroglucinol. The DPPH radical scavenging activities of the compounds were investigated., Results: The new trimeric prodelphinidin, epigallocatechin-(4β→8)-epigallocatechin-(4β→8)-catechin (compound 7), has been isolated from the air-dried leaves of the title plant, in addition to catechin, epigallocatechin, gallocatechin, and three dimeric proanthocyanidins. The dimeric prodelphinidin epigallocatechin-(4β→8)-epigallocatechin (compound 6) showed the strongest DPPH radical scavenging activity, with IC(50) 1.7 μg/mL, 10 times more active than the positive control, BHT (IC(50) 17.3 µg/mL), followed by the new trimeric proanthocyanidin epigallocatechin-(4β→8)-epigallocatechin-(4β→8)-catechin with IC(50) 2.1 µg/mL. The crude extract exhibited high DPPH radical scavenging activity with IC(50) 15.5 µg/mL comparable with that of BHT., Discussion and Conclusion: The results showed that all the isolated compounds from the tannin fraction exhibited potent free radical scavenging activities, which were higher than that of BHT, suggesting that the condensed tannins from G. biloba leaves strongly contribute to the overall antioxidant effects.
- Published
- 2011
- Full Text
- View/download PDF
14. Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1.
- Author
-
Abdel-Malek SM, Al-Adham IS, Winder CL, Buultjens TE, Gartland KM, and Collier PJ
- Subjects
- Animals, Cetrimonium, Cetrimonium Compounds pharmacology, Culture Media, Drug Resistance, Bacterial, Microbial Sensitivity Tests, Organometallic Compounds pharmacology, Thiones, Bacterial Outer Membrane Proteins metabolism, Plankton drug effects, Plankton growth & development, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa growth & development, Pyridines pharmacology
- Abstract
Aims: The aim of this study was to determine whether passaging Pseudomonas aeruginosa PAO1 with sub-MICs of the pyrithione biocides results in both the induction of decreased susceptibility towards these antimicrobials and associated outer membrane profile changes., Methods and Results: Previous work by this group has shown that it is possible to induce susceptibility changes towards the isothiazolone biocides in Ps. aeruginosa PAO1 by successive passages in the presence of increasing sub-MICs of biocide. This procedure was accompanied by the loss of a 35 kDa outer membrane protein, T-OMP. In this experiment, this process was repeated with the biocides sodium pyrithione (NaPT), zinc pyrithione (ZnPT) and cetrimide. The pattern of susceptibility was similar to that observed with the isothiazolone biocides. Upon removal of biocide, the observed MIC did not return to the original pre-exposure value. The onset and development of resistance was accompanied by the loss of T-OMP from outer membrane profiles, which suggests that this is a non-specific membrane channel whose production within the cell is sensitive to biocide presence. The T-OMP reappeared when the cells were passaged in the absence of pyrithione. Cross-resistance studies indicated that induced resistance to one biocide yields partial resistance towards other members of the group and the positive control., Conclusions: These results indicate that the pyrithione biocides have similar susceptibility profiles in Ps. aeruginosa to those exhibited by the isothiazolones, but that the acquired changes in susceptibility to the pyrithiones is largely irreversible., Significance and Impact of the Study: This study indicates that acquired susceptibility changes towards sub-MICs of selected biocides are multifactorial in nature.
- Published
- 2002
- Full Text
- View/download PDF
15. Pyrithione biocide interactions with bacterial phospholipid head groups.
- Author
-
Dinning AJ, Al-Adham IS, Austin P, Charlton M, and Collier PJ
- Subjects
- Antifungal Agents chemistry, Edetic Acid metabolism, Models, Molecular, Phospholipids chemistry, Pseudomonas aeruginosa chemistry, Pyridines chemistry, Spectrophotometry, Thiones, Antifungal Agents metabolism, Escherichia coli metabolism, Organometallic Compounds metabolism, Phospholipids metabolism, Pseudomonas aeruginosa metabolism, Pyridines metabolism
- Abstract
Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are antimicrobial agents widely used in both the cosmetics and fuel industries. They are also utilized in the mining industry because of their metal chelating properties. They have been shown to depolarize membrane electropotential in fungi and are also known to inhibit fungal and bacterial substrate transport processes. Recent work has shown that both pyrithiones cause the leakage of intracellular material (potassium ions and O.D.260 nm absorbing material) from exposed bacterial cells. The work here reports studies on the interactions between the pyrithiones and the bacterial phospholipid head group structures, at both a practical and a theoretical level, utilizing tube dilution neutralizer studies, scanning spectrophotometry and molecular modelling. The tube dilution neutralizer studies exhibited a decrease in minimum inhibitory concentration (MIC) for both pyrithiones in the presence of extracellular phosphatidyl-ethanolamine and EDTA. Scanning spectrophotometry exhibited the chelation of the central zinc atom from the ZnPT chelate by the addition of EDTA. Molecular modelling studies exhibited the chelation of the phosphatidyl-ethanolamine head group by ZnPT. Zinc pyrithione also exhibited an interaction with the ammonium tail of the head group structures. Sodium pyrithione exhibited electrostatic interactions with the phospholipid head groups in the molecular modelling studies.
- Published
- 1998
- Full Text
- View/download PDF
16. Pyrithione biocides as inhibitors of bacterial ATP synthesis.
- Author
-
Dinning AJ, Al-Adham IS, Eastwood IM, Austin P, and Collier PJ
- Subjects
- Escherichia coli drug effects, Escherichia coli metabolism, Microbial Sensitivity Tests, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa metabolism, Thiones, Time Factors, Adenosine Triphosphate biosynthesis, Antifungal Agents pharmacology, Organometallic Compounds pharmacology, Pyridines pharmacology
- Abstract
Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and general antimicrobial agents. They have been shown to be active against fungal cell walls, associated membranes and bacterial transport processes. Investigations were undertaken into the effect of these antimicrobial agents on substrate catabolism and intracellular ATP levels using an oxygen electrode and luciferin-laciferase technology, respectively. Results indicate that, while both compounds are poor inhibitors of substrate catabolism, sub-inhibitory concentrations of biocide greatly reduces intracellular ATP levels in both Escherichia coli NCIMB 10000 and Pseudomonas aeruginosa NCIMB 10548. This is thought to be due to the action of NaPT and ZnPT on the Gram-negative bacterial membrane.
- Published
- 1998
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.