Search

Background/Aim: Previous studies on "Black seed" or "Black Cumin" Nigella sativa (NS) have reported a large number of pharmacological activities including its anti-ulcer potential. These studies employed either fixed oil, volatile oil components or different solvent extracts. In folkloric practices, NS seeds are taken as such, in the form of coarse dry powder or the powdered seeds are mixed with water. This study examines the effect of NS aqueous suspension on experimentally induced gastric ulcers and basal gastric secretion in rats to rationalize its use by herbal and Unani medicine practitioners. Materials and Methods: The study was conducted at the Medicinal, Aromatic and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Acute gastric ulceration was produced by various noxious chemicals (80% ethanol, 0.2 M NaOH, 25% NaCl and indomethacin) in Wistar albino rats. Anti-secretory studies were undertaken in a separate group of rats. Gastric wall mucus contents and non-protein sulfhydryl concentration were estimated, and gastric tissue was examined histopathologically. Results: An aqueous suspension of Black seed significantly prevented gastric ulcer formation induced by necrotizing agents. It also significantly ameliorated the ulcer severity and basal gastric acid secretion in pylorus-ligated Shay rats. Moreover, the suspension significantly replenished the ethanol-induced depleted gastric wall mucus content levels and gastric mucosal non-protein sulfhydryl concentration. The anti-ulcer effect was further confirmed histopathologically. Conclusion: These findings validate the use of Black seed in gastropathies induced by necrotizing agents. The anti-ulcer effect of NS is possibly prostaglandin-mediated and/or through its antioxidant and anti-secretory activities.

Background: Red eye is one of the most important topics in medical practice. It is frequently encountered in primary health care and emergency departments. It is usually marked by an ocular inflammation. It is often considered as benign but the condition entails a vast array of differential diagnoses with some being considered as medical emergencies and so require urgent action. Objectives: In this paper, we will review the studies relevant to diagnoses, differential diagnosis, clinical features, and management of diseases that may cause red eye presentation. Methodology: PubMed database was used for articles selection using the keywords Red Eyes, and Evaluation. Conclusion: Family physicians need to have basic understanding of the management of the main diagnoses behind red eye. They also should know when to refer the patients to a higher center. Early diagnosing and recognition of the disease is important for early intervention or quick referral to an ophthalmologist. It can be even sight saving in a lot of patients. Further studies into the management of more common diseases such as conjunctivitis with larger sample studies and longer follow up duration can help in the future. [ABSTRACT FROM AUTHOR]

This study was designed to investigate the possible antiniciceptive, antipyretic and antimicrobial activities of the essential oil obtained from the fruits of Piper Cubeba (L.). To assess the antinociceptive and antipyretic activities, three doses (150, 300 and 600 mg/kg, i.p.) were tested in acetic acid-induced abdominal writhing, tail flick reaction and hot-plate and Brewer's yeast-induced hyperpyrexia test models in animals. Moreover, the antimicrobial activity was examined using agar diffusion method and broth micro-dilution assay for minimum inhibitory concentrations (MIC). The Piper Cubeba essential oil (PCEO) showed a marked antinociception (17, 30 and 54%) and an increase in reaction time in mice in the flick tailed and hot-plate tests. The brewer's yeast induced hyperpyrexia was decreased in a dose dependent manner. PCEO also exhibited a strong antimicrobial potential. These findings confirm the traditional analgesic indications of P. cubeba oil and provide persuasive evidence and support its use in Arab traditional medicine. [ABSTRACT FROM AUTHOR]

Background. Liver diseases still represent a major health burden worldwide. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. Thus, the present study was to evaluate the effectiveness of Piper cubeba fruits in the amelioration of CCl4-induced liver injuries and oxidative damage in the rodent model. Methods. Hepatoprotective activity was assessed using various biochemical parameters like SGOT, SGPT, γ-GGT, ALP, total bilirubin, LDH, and total protein. Meanwhile, in vivo antioxidant activities as LPO, NP-SH, and CAT were measured in rat liver as well as mRNA expression of cytokines such as TNFα, IL-6, and IL-10 and stress related genes iNOS and HO-1 were determined by RT-PCR. The extent of liver damage was also analyzed through histopathological observations. Results. Treatment with PCEE significantly and dose dependently prevented drug induced increase in serum levels of hepatic enzymes. Furthermore, PCEE significantly reduced the lipid peroxidation in the liver tissue and restored activities of defense antioxidant enzymes NP-SH and CAT towards normal levels. The administration of PCEE significantly downregulated the CCl4-induced proinflammatory cytokines TNFα and IL-6 mRNA expression in dose dependent manner, while it upregulated the IL-10 and induced hepatoprotective effect by downregulating mRNA expression of iNOS and HO-1 gene. [ABSTRACT FROM AUTHOR]

Phytochemical investigation of the aerial part of Ononis natrix L. led to the isolation of a new compound, 6-(2'R-acetoxypentadecyl)-2-hydroxy-4-methoxybenzoic acid (1), along with twenty one known compounds. The structures of the compounds were elucidated by standard spectroscopic methods. These compounds were screened for cytotoxic, antioxidant, antimicrobial, antimalarial, antileishmanial and antitrypanosomal activities using in vitro assays. [ABSTRACT FROM AUTHOR]

The essential oil of Haplophyllum tuberculatumwas prepared by hydrodistillation of the fresh flowering aerial parts of the plant collected from Saudi Arabia. The oil was subsequently analyzed by GC and GC-MS. Thirty seven compounds, accounting for 96.4% of the oil composition were identified. The major components were trans-p-menth-2-en-1-ol (19.2%), cis-p-menth-2-en-1-ol (13.2%), myrcene (10.1%), d-3- carene (8.8%), β-phellandrene (6.9%), limonene (6.6%) and cis-piperitol (6.4%). The antimicrobial activity of the essential oil was determined using the broth microdilution method against various human pathogens, where a relatively low inhibitory range was observed (MIC 1 mg/mL). Furthermore, the oil was evaluated for its antifungal activity against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides using the direct overlay bioautography assay. The essential oil showed no antifungal activity at 80 and 160 µg/spot concentrations compared to commercial antifungal standards. The oil was also investigated for its insecticidal and repellent activity against Aedes aegypti. The oil was repellent to the yellow fever mosquito Ae. aegypti using the "cloth patch assay" down to a concentration of 0.074 mg/cm2; however, the oil had low toxicity against first instar larvae and adults of Ae. aegypti in a high throughput larval bioassay and adult topical assay. Additionally, enzyme activity was measured using the spectrophotometric Ellman method. The oil showed weakly acetylcholinesterase (AChE) inhibitory activity at the tested concentration, compared to standard substances, whereas no inhibition on butyrylcholinesterase (BuChE) activity was observed. [ABSTRACT FROM AUTHOR]

Thepresent investigation was designed to investigate the protective effect of (Beta vulgaris L.) beat root ethanolic extract (BVEE) on gentamicin-induced nephrotoxicity and to elucidate the potential mechanism. Serum specific kidney function parameters (urea, uric acid, total protein, creatinine, and histopathology of kidney tissue)were evaluated to access gentamicin-induced nephrotoxicity. The oxidative/nitrosative stress (Lipid peroxidation, MDA, NP-SH, Catalase, and nitric oxide levels) was assessed.The inflammatory response (TNF-α, IL-6, MPO, NF-κB (p65), and NF-κB (p65) DNA binding) and apoptotic marker (Caspase-3, Bax, and Bcl- 2) were also evaluated. BVEE (250 and 500mg/kg) treatment along with gentamicin restored/increased the renal endogenous antioxidant status. Gentamicin-induced increased renal inflammatory cytokines (TNF-α and IL-6), nuclear protein expression of NF-κB (p65), NF-κB-DNA binding activity, myeloperoxidase (MPO) activity, and nitric oxide level were significantly down regulated upon BVEE treatment. In addition, BVEE treatment significantly reduced the amount of cleaved caspase 3 and Bax, protein expression and increased the Bcl-2 protein expression. BVEE treatment also ameliorated the extent of histologic injury and reduced inflammatory infiltration in renal tubules. These findings suggest that BVEE treatment attenuates renal dysfunction and structural damage through the reduction of oxidative stress, inflammation, and apoptosis in the kidney. [ABSTRACT FROM AUTHOR]