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A prospective study on 193 patients through 3 years from February 2020 till January 2023 patients complain of colorectal carcinoma in different locations. 43 patients (23%) had a history of open or laparoscopic cholecystectomy since a different time ranging from 9 to more than 30 years, who have no family history of colorectal cancer or inflammatory bowel diseases, adenomatous polyps, and no history of familial adenomatous polyposis, (predisposing factors of colorectal cancer). most common location of malignancy for those patients with a history of cholecystectomy in the RT side of the colon 21 patients (48.8%) and the least location in the ano-rectum region one patient (2.4%). The most common time that we diagnosed colorectal carcinoma after 21 to 25 years from the time of cholecystectomy which occurred in 18 patients (41.9%) and the least after 9 years only one patient (2.4). While the microscopic appearance of colorectal cancer in patients with a history of cholecystectomy is, adenocarcinoma occurs in 38 patients (88.4%) and squamous carcinoma occurs in 5 patients (11.6%). While the macroscopic appearance of colorectal cancer occurs most commonly in ulcerative types in 29 patients (67.5%) and least annular type 5 patients (11.6%) and the most common age occur between the age of 60 and 69 years in 24 patients (55.8%) and leas occur at the age of 40 to 49 years only 2 patients (4.6%), about the grading of the tumor moderate differentiated type, is the most common type in 67.5% Aim of the study to know the incidence of colorectal carcinoma after cholecystectomy, locations of tumors, and related time. [ABSTRACT FROM AUTHOR]

Abdominal closure after laparotomy takes several weeks to get 80% of its previous strength in this period the fascial sheath should be hold by sutures. Several materials used in the abdominal closures but the most common suture used is polypropylene, others used nylon, less common used is absorbable sutures but wound sinus is a complication may fellow the use of non-absorbable sutures. For two years we collect 235 patients in all of them abdominal wall incision was Pfannenstiel, in 133 patients the incision closed by polypropylene (non-absorbable), for other 102 patients the incision closed by polydioxanone (slow absorbable) the result was significant for wound sinus formation in polypropylene group but insignificant for hernia, conclusion of the study the polydioxanone look the same strength of polypropylene and may be without wound sinus formation. [ABSTRACT FROM AUTHOR]

Of the most prevalent infections globally are urinary tract infection and one of the main and common causes of this infection is bacterium Uropathogenic Escherichia coli (UPEC). In the present study, virulence factors and antibiotic resistance as well as phylogenetic groups were investigated in UPEC among UTIs in general. One hundred and five midstream urine samples have been from pregnant women suffering from (UTI), aged between (17-40) years from Al-Kut hospital for Gynecology obstetrics and pediatrics and private clinics in the province of Wasit, during the period from 1st September, 2020 to 15th December, 2020. The percentage of bacterial growth which was isolated from 105 samples, 36.1% was UPEC .The results of culture and biochemical tests showed that all E.coli isolates were had given positive test for catalase, indole, Methyl Red, but negative for oxidase, Voges-Proskauer, Simmon citrate and TSI test showed A/A with gas, without H2S. Further confirmation done using API 20E system depending on 20 biochemical tests related with E. coli metabolism activities and Vitek 2. The antibiotic susceptibility test for 38 of UTI Escherichia coli isolates, were performed by disc diffusion method, using 18 antibiotics and their results showed the highest antibiotic resistance were Ampicillin, (97.2%), amoxicillin-clavulanic acid (86.4%), followed by cefotaxime (78.3%), while lowest resist antibiotic were gentamicin (29.7%), Amikacin (27.0%), Nitrofurantoin (13.5%), Meropenem (5.4%), Imipenem (2.7%), this may be a result of the low utilization of those antibiotics in Al-Kut hospitals. DNA extraction of isolates was extracted, purity and concentration were confirmed with Nanodrop, the purity of the nucleic acid in the samples ranged between 1.8-2, while its concentration ranged from (50-360 mg / ìl). In this study, phylogenetic groups of UPEC were identified. A quadraplex PCR assay was applied to detect phylogroups' reference of E. coli isolates (n=38), the maximal frequency of UPEC isolates has been in group B2 (23.6%) then group F (15.7%), group B1 (13.1%), group A (10. 5%), D (7.8%) and group C (2.6%). Groups E has not been detected in any of the isolates, and 6 isolates have been non typeable. Virulence factors' genes that were detected among E. coli isolates by PCR were cnf (6:15.7%), hly (13:34.2%). Other Virulence factors' genes were detected by multiplexed PCR such as fimH (18:47.3%), papC (13:34.2%), sfa (12:31.5%), afa (8:21.0%) and aer (16:42.1%). The current study proved that the isolates, have high antibiotic resistance with a dominance of B2 group. As for the virulence genes for this pathogen, the fimH gene was the most common, followed by the aer, hly, pap, sfa, afa and cnf genes, respectively [ABSTRACT FROM AUTHOR]

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