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Sexual dimorphism in the human species is key to the development of sex estimation techniques in the human skeleton. This dimorphism is manifested, as in other regions of the skeleton, in the bones that constitute the thoracic cage, according to the existing bibliography. In this aspect, the study of the human skeleton through 3D images has also proved to be useful for the development and validation of sex estimation methodologies for the reconstruction of the osteobiological profile.For this purpose, a sample of 240 thoracic CT scans of adult individuals was selected from a collection of 3D images belonging to the University of Granada, provided by the Castilla-La Mancha Health Service (SESCAM). Different measurements of the thoracic bones (ribs R2 to R5 width, sternum length and width, and clavicles width) have been taken with OsiriX software, with the aim of developing discriminant functions for sex estimation.The obtained results are positive, allowing sex estimation through 3D images of the thorax with up to 89.6% accuracy through discriminant functions, which shows the usefulness of image analysis for the reconstruction of the osteobiological profile., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Age estimation is crucial in legal and humanitarian contexts. Forensic professionals may use various procedures to estimate age, including dental analysis, bone density tests, evaluation of physical characteristics including facial bone structure and development, and image-based methods. Although images are often the only material available, visual observation of photographic material is an imprecise method in age estimation, which can compromise judicial decision-making. Analyzing 4000 photographs from the Brazilian Federal Police database, representing four age groups (6, 10, 14, and 18 years), the study employed automated analysis by marking 28 photogrammetric points. Data were used to establish facial patterns by age and sex using the facial geometric morphometrics method. Performance was assessed through a Multinomial Logistic Regression model, evaluating accuracy, sensitivity, and specificity across the categorical age groups. Analyses were conducted using R software, with a 5 % significance level. The study found that facial geometric morphometrics achieved an overall accuracy of 69.3 % in age discrimination, with higher accuracy in males (74.7 %) compared to females (65.8 %) (p < 0.001). The method excelled at predicting the age of 6-year-olds with 87.3 % sensitivity and 95.6 % specificity but had lower performance at 14 years. It showed greater accuracy in distinguishing age groups with larger age gaps, achieving up to 99.5 % accuracy between certain groups, and was particularly effective in differentiating ages of 6 and 10 years in females and 10, 14, and 18 years in males. The facial geometric morphometrics emerges as a promising approach for age estimation among children and adolescents in forensic settings., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.)

This study proposes an assessment of the accuracy of the Fazekas and Kósa and Nagaoka methods by measuring the squamosal and petrous portions of the temporal bone, whose application in the Mediterranean population is not recommended. Therefore, our proposal is a new formula to estimate the age of skeletal remains from individuals at 5 months gestational age to 1.5 postnatal years with the temporal bone. The proposed equation was calculated on a Mediterranean sample identified from the cemetery of San José, Granada (n = 109). The mathematical model used is the exponential regression of the estimated age for each measure and sex, and both in combination, using an inverse calibration and cross-validation model. In addition, the estimation errors and the percentage of individuals within a 95% confidence interval were calculated. The lateral development of the skull, especially the growth of the length of the petrous portion, showed the greatest accuracy, while its counterpart, the width of the pars petrosa, showed the lowest accuracy, so its use is discouraged. The positive results from this paper should be useful in both forensic and bioarchaeological contexts., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Sexual dimorphism is the basis for sex estimation research in skeletal remains, helping the positive identification of individuals in forensic backgrounds. In this regard, it has been proved that the metrical analysis of the costal neck of the first four ribs in Spanish contemporary adult skeletons shows high sexual dimorphism, especially in the first rib. However, the validation of any method developed in identified skeletal collections must be compulsorily reproduced in other skeletonized sets to verify either its potential application in individuals from other locations, or the existence of possible biases associated with inter-population variation. Due to the geographical and socioeconomic proximity between Spain and Portugal, this paper aims to check the utility of the discriminant functions designed by Partido-Navadijo et al. (2021) in the two skeletal collections of the University of Coimbra (Portugal). Results show the utility of these discriminant functions in Portuguese population, with frequencies of cases correctly assigned reaching up to 93.6% in the Coimbra Identified Skeletal Collection (CISC); and up to 92.6% in the 21 st Century Identified Skeletal Collection (CEI/XXI). However, two particularities between the Spanish and Portuguese osteological assemblages can be highlighted: the possible existence of secular changes in relation to the CISC collection (1904-1938); and a reversal in the degree of costal dimorphism, being the third and fourth ribs of the CEI/XXI collection more dimorphic than the first ones., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Age estimation is essential for the identification of skeletal remains in Forensic Anthropology. Numerous studies have been performed on diverse regions of the skeleton, including the synostosis of the sternal segments. In this sense, the fusion of the different sternal segments was assessed to analyze whether it had a correlation with age-at-death in a Mediterranean population. A total of 189 sternums which belonged to individuals between the ages of 20 and 98 from both sexes (56.6% males; 43.4% females), from the San José's Cemetery of Granada Contemporary Collection (Spain), were selected. Scores ranging from 1 to 3 were assigned in accordance with the degree of manubrio-sternal fusion and sterno-xyphoidal fusion. Cohen's kappa coefficient for intra- and inter-observer error was performed and then chi-square test was run to analyze any correlation between the stage of synostosis and the skeletal age. Only the sterno-xyphoidal fusion, which starts between 30 and 39 years old, provided a predictable result, as the manubrium and the sternal body usually remain without fusion. This study demonstrates a direct correlation between fusion of the xyphoid process and chronological age., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

The objectives of this work were to validate two published methods for subadult age estimation based on measurements of the pars lateralis, and to develop a new method based on a wider set of measurements using the Granada Osteological Collection. The pars lateralis of 127 individuals from 6 months prenatal to 4 years of age were measured, taking 6 measurements of the body, the anterior synchondrosis and the condyle. Length and width were used to validate the published methods. Regression functions using age as the independent variable were calculated using each of the six measurements taken, and functions for age estimation were obtained through classical calibration. Functions for calculation of the 95% confidence interval of the estimates were obtained through linear regression using the estimation errors. In the validation of the previous methods, one method showed a linear tendency of the differences, which can be attributed to a circularity in reasoning in the original work. In the other method, a tendency towards overestimation was found, which can be attributed to the limitations of the method itself. The new functions have a consistency rate of 92.2% to 97.1%; the functions derived from all measurements are useful from 6 months prenatal to 2 years of age. Moreover, the functions obtained are applicable to incomplete pars lateralis, allowing for age estimation in a wide set of contexts and providing straightforward age estimates with their respective margin of error., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Pre- and postnatal development and variability in discrete vertebral traits have been poorly described in embryonic studies. Numerous authors have reported that these variations are observable only from adolescence; scientific publications on the vertebrae of fetuses and infants are scarce. Thus, the aims of this study were to (1) describe the ontogeny and variability of anatomical variations in the vertebral column of a Spanish infant population and (2) analyze the frequency and relationship between sex, age, and intertrait variables. A total of 4728 vertebrae from 197 skeletons were studied. The age at death ranged from 22 intrauterine weeks to 8 years. Twenty morphological traits related to vertebral column development were analyzed. A descriptive statistical analysis was performed, and the chi-square test was used to measure the relationship between sex, age, and intertrait variables. We observed that 88.32% of skeletons expressed discrete traits along the spine. In fetuses, the double transverse foramen and unclosed transverse process of the axis were the most prevalent traits. In infants older than one year, the appearance of the L5 cleft neural arch, unclosed transverse process of the atlas, and craniocaudal shifts were frequent. A significant result was found between sex and the unclosed transverse process in the axis. The intertrait relationship was significant for all traits that shared the same embryonic structure. Morphological variations became visible following the appearance of ossification centers during the pre- and postnatal periods, and their etiology was associated with embryonic development., Competing Interests: Authors declaration of conflicts of interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier GmbH. All rights reserved.)

Age-at-death estimation from skeletal remains typically utilizes the roughness of pubic symphysis articular surfaces. This study presents a new quantitative method adapting a tool from geometric morphometrics, bandpass filtering of partial warp bending energy to extract only age-related changes of the surfaces. The study sample consisted of 440 surface-scanned symphyseal pubic bones from men between 14 and 82 years of age, which were landmarked with 102 fixed and surface semilandmarks. From the original sample, 371 specimens within Procrustes distance of 0.05 of the side-specific average were selected. For this subsample, age was correlated with total bending energy (calculated as summed squared partial warps amplitudes) for a wide range of plausible bandpass filters. For our subsample's 188 right-side surfaces, the correlation between age and bandpass filtered versions of bending energy peaks relatively sharply at r = -0.648 for ages up through 49 years against the first seven partial warp amplitudes only. The finding for left symphyses is similar. The results demonstrate that below the age 50, the symphyseal surface form changes most systematically related to age may be best detected by a lowpass-filtered version of bending energy: signals at the largest geometric scales of roughness rather than its full spectrum. Combining this method with information from other skeletal features could further improve age-at-death estimation based on the symphyseal pubic surface., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

One of the first steps in the identification of human remains is sex estimation. Discriminant functions are very useful for this purpose, as they are based on the fundamental premise of the sexual dimorphism in osseous remains. However, the absence of studies of this kind in the thoracic cage is visible, and adequate cause to justify this work. To this purpose, we analysed and measured a set of ribs, R1 to R4, belonging to skeletons of contemporary chronology (1970-2010), in order to obtain discriminant functions enabling sex estimation through equations applied to the rib neck. The sample comes from the Cemetery of San José, which is located at the Laboratory of Anthropology at the University of Granada. All data from this sample was known: ancestry, sex, age-at-death and cause of death. Results showed a percentage of success of approximately 80-88% in ribs R2 to R4, and approximately 85-90% in R1, reaching up to 93.2%. Results from intra-/inter-observer error tests show excellent reproducibility of the method. The data confirms that it is possible to estimate sex from the rib neck.

In the identification and reconstruction of the biological profile of skeletal remains in physical and forensic anthropology, sex estimation is a fundamental step, and is essential to achieve methods that allow the highest allocation accuracy. This study proposes a method to estimate the sex of skeletal remains using lumbar vertebrae. A total of 33 linear measurements for each lumbar vertebra were taken in 94 identified, contemporary, and adult skeletal remains (46 male and 48 female) from the cemetery of San José in Granada. The sexual dimorphism of each variable was analyzed, an intra- and interobserver error analysis was developed to assess the variables with best concordance, and the discriminant equations were obtained through a binary logistic regression analysis. All lumbar vertebrae show statistically significant sexual dimorphism with higher values in males than in females. Equations with an allocation accuracy of between 80% and 94.5% were obtained, showing that the lumbar vertebrae are a useful alternative for sex estimation when other skeletal elements are not well-preserved or available. The differences of biogeographic history between populations could translate into morphometric differences, which means that it is not advisable to use discriminant equations in a universal way. In spite of the high allocation accuracy of the equations developed, they should be applied in adults from populations similar to the present study to obtain reliable results of sexual estimation, until its validation in other populations.

Objective: To interpret traumatic cranial lesions on two individuals buried at the Neolithic necropolis of Campo de Hockey, Spain., Materials: Skeletal remains and grave goods associated with two individuals recovered in good condition from a single grave., Methods: Crania were macroscopically and radiographically analysed., Results: Of all the individuals recovered from this site, only two present perimortem skull injuries. Fractures were located on the right side of the skulls, one on the frontal bone and the other on the parietal bone., Conclusions: The individuals present signs of interpersonal violence. In both cases, the injuries are perimortem and may have contributed to the deaths of the individuals., Significance: The identification of interpersonal violence in the two individuals buried in the tomb, alongside the archaeological context of the burials, provides unique insight into Neolithic burial practices in the Iberian Peninsula and the role that paleopathology can play in the recognition of social complexity., Limitations: The skeletal remains of the two individuals were fragmented, rendering the identification of perimortem lesions difficult., Suggestions for Further Research: New imaging techniques, including CT-scan, will provide more detailed analyses., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Sex determination is one of the essential steps when it comes to establishing an individual's biological profile. It is important in both archaeology and forensic studies. The sacrum is not generally conserved, but in cases where it is, it can be used for determination of the sex of skeletal remains. Furthermore, the sacrum is not a commonly studied bone and has not been studied in a Spanish population. For this study, measurements of 170 sacra of individuals from the contemporary osteological collection of San José from Granada including only the adults were taken. Measurements based on the anatomical regions of the sacra were established in order to obtain some regression formulas to determine sex. Our results show that the Superior Transverse Line and Right Lateral Sacral Crest are the most dimorphic structures, achieving a 74% rate of correct classification of sex in a univariate analysis. In multivariate analysis, when the mentioned variables were combined, an 81.41% rate of correct classification was achieved. Our results show that our method can be applied with other methods at the same time to determine the sex of individuals in forensic and archaeological contexts., (Copyright © 2018 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.)

Sex determination of unknown individuals is one of the primary goals of Physical and Forensic Anthropology. The adult skeleton can be sexed using both morphological and metric traits on a large number of bones. The human pelvis is often used as an important element of adult sex determination. However, studies carried out about the pelvic bone in subadult individuals present several limitations due the absence of sexually dimorphic characteristics. In this study, we analyse the sexual dimorphism of the immature pubis and ischium bones, attending to their shape (Procrustes residuals) and size (centroid size), using an identified sample of subadult individuals composed of 58 individuals for the pubis and 83 for the ischium, aged between birth and 1year of life, from the Granada osteological collection of identified infants (Granada, Spain). Geometric morphometric methods and discriminant analysis were applied to this study. The results of intra- and inter-observer error showed good and excellent agreement in the location of coordinates of landmarks and semilandmarks, respectively. Principal component analysis performed on shape and size variables showed superposition of the two sexes, suggesting a low degree of sexual dimorphism. Canonical variable analysis did not show significant changes between the male and female shapes. As a consequence, discriminant analysis with leave-one-out cross validation provided low classification accuracy. The results suggested a low degree of sexual dimorphism supported by significant sexual dimorphism in the subadult sample and poor cross-validated classification accuracy. The inclusion of centroid size as a discriminant variable does not imply a significant improvement in the results of the analysis. The similarities found between the sexes prevent consideration of pubic and ischial morphology as a sex estimator in early stages of development. The authors suggest extending this study by analysing the different trajectories of shape and size in later ontogeny between males and females., (Copyright © 2018 Elsevier B.V. All rights reserved.)

In the context of physical and forensic anthropology, when a child's skeleton is damaged or in poor condition, which is common, many of the metric methods for the estimation of skeletal age cannot be used. In these circumstances, those more resistant bones, such as the pars basilaris, will be most useful. The aims of this study were to test existing methods for estimating skeletal age from the metric study of the pars basilaris and to propose new regression formulae. One hundred fourteen individuals aged between 5 months of gestation and 6 years were analyzed; seven measures were taken from each pars basilaris using a digital caliper. The chronological age was compared with the estimated age using the methods published by Fazekas and Kósa in 1978 and by Scheuer and MacLaughlin in 1994. New regression formulae are proposed, obtained by classical calibration, which include confidence intervals at 50 and 97.5 % to express the error. With both methods, significant differences were observed; the method of Fazekas and Kósa shows a tendency to underestimate the age, and the method of Scheuer and MacLaughlin tends to overestimate it. The proposed formulae represent a good tool for estimating age in many different contexts because they are relatively easy to apply, although other analysis systems, such as Bayesian approach or geometric morphometry, offer more robust and effective results.

Sex estimation of juveniles in the Physical and Forensic Anthropology context is currently a task with serious difficulties because the discriminatory bone characteristics are minimal until puberty. Also, the small number of osteological collections of children available for research has made it difficult to develop effective methodologies in this regard. This study tested the characteristics of the ilium and jaw proposed by Schutkowski in 1993 for estimation of sex in subadults. The study sample consisted of 109 boys and 76 girls, ranging in age from 5 months of gestation to 6 years, from the identified osteological collection of Granada (Spain). For the analysis and interpretation of the results, we have proposed changes from previous studies because we believe they raised methodological errors relating to the calculation of probabilities of success and sex distribution in the sample. The results showed correct assignment probabilities much lower than those obtained by Schutkowski as well as by other authors. The best results were obtained with the angle and depth of the sciatic notch, with 0.73 and 0.80 probability of correct assignment respectively if the male trait was observed. The results obtained with the other criteria were too small to be valid in the context of Physical or Forensic Anthropology. From our results, we concluded that Schutkowski method should not be used in forensic context, and that the sciatic notch is the most dimorphic trait in subadults and, therefore, the most appropriate to develop more effective methods for estimating sex.

The methodology used to identify individuals in forensic anthropology requires a minimum degree of precision and accuracy and should be based on identified and representative samples. Achievement of these objectives in infant skeletons is hampered by the scarcity of appropriate samples. The dental age estimation methods of Liversidge et al. (Am J Phys Anthropol 90: 307-313, 1993) and Deutsch et al. (Growth 49: 207-17, 1985) were applied to the Granada osteological collection of identified infants (Granada, Spain) in order to evaluate its applicability in a Mediterranean population. Significant differences were found between the estimated and real ages in both cases. Based on the measurements obtained in 140 fetuses and infants, new regression formulas were developed to estimate age from the metric study on deciduous teeth. Independent functions are provided for each deciduous maxillary and mandibular tooth in each sex, along with the margin of error (95% confidence interval). These formulas appear to offer one of the best methods available for estimating the age of Mediterranean infants in forensic anthropology settings.

Radiology has demonstrated great utility for sex determination, but most studies are based in metrical and morphological methods in order to perform an identification profile. It is presented an easy image analysis-based method aimed to demonstrate the relationship between the bony tissue ultrastructure and sex by using several grey level histogram variables obtained from computed tomography images using sagittal sections of the pubic symphysis surface and the body of the pubis. The CT sample consisted of 169 hospital DICOM archives of known sex and age. The multivariate binary logistic regression models calculated showed reliabilities for sex determination between 87.4 and 95.8 %, with a high intra and inter-observer agreement. The suggested method is not only considered useful to perform an identification profile during Virtopsy, but even for application in further studies in order to attach a quantitative correlation for tissue ultrastructure characteristics without complex and expensive methods beyond image analysis.