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1. Trpv6 channel targeting using monoclonal antibody induces prostate cancer cell apoptosis and tumor regression

Author

Aurélien Haustrate, Clément Cordier, George Shapovalov, Adriana Mihalache, Emilie Desruelles, Benjamin Soret, Nadège Charlène Essonghé, Corentin Spriet, Maya Yassine, Alexandre Barras, Johanna Marines, Lindsay B. Alcaraz, Sabine Szunerits, Gautier Robin, Pierre Gosset, Natalia Prevarskaya, and V’yacheslav Lehen’kyi

Subjects
Cytology, QH573-671
Abstract

Abstract TRPV6 calcium channel is a prospective target in prostate cancer (PCa) since it is not expressed in healthy prostate while its expression increases during cancer progression. Despite the role of TRPV6 in PCa cell survival and apoptotic resistance has been already established, no reliable tool to target TRPV6 channel in vivo and thus to reduce tumor burden is known to date. Here we report the generation of mouse monoclonal antibody mAb82 raised against extracellular epitope of the pore region of the channel. mAb82 inhibited TRPV6 currents by 90% at 24 µg/ml in a dose-dependent manner while decreasing store-operated calcium entry to 56% at only 2.4 µg/ml. mAb82 decreased PCa survival rate in vitro by 71% at 12 µg/ml via inducing cell death through the apoptosis cascade via activation of the protease calpain, following bax activation, mitochondria enlargement, and loss of cristae, Cyt C release, pro-caspase 9 cleavage with the subsequent activation of caspases 3/7. In vivo, mice bearing either PC3M trpv6+/+ or PC3M trpv6-/- +pTRPV6 tumors were successfully treated with mAb82 at the dose as low as 100 µg/kg resulting in a significant reduction tumor growth by 31% and 90%, respectively. The survival rate was markedly improved by 3.5 times in mice treated with mAb82 in PC3M trpv6+/+ tumor group and completely restored in PC3M trpv6-/- +pTRPV6 tumor group. mAb82 showed a TRPV6-expression dependent organ distribution and virtually no toxicity in the same way as mAbAU1, a control antibody of the same Ig2a isotype. Overall, our data demonstrate for the first time the use of an anti-TRPV6 monoclonal antibody in vitro and in vivo in the treatment of the TRPV6-expressing PCa tumors.

Published
2024
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4. Polymer-Based Nanostructures for Pancreatic Beta-Cell Imaging and Non-Invasive Treatment of Diabetes

Author

Shakila Behzadifar, Alexandre Barras, Valérie Plaisance, Valérie Pawlowski, Sabine Szunerits, Amar Abderrahmani, and Rabah Boukherroub

Subjects
diabetes, beta cells mass, polymer-based nanostructures, imaging, antidiabetic drug delivery, foot ulcers, Pharmacy and materia medica, RS1-441
Abstract

Diabetes poses major economic, social, and public health challenges in all countries worldwide. Besides cardiovascular disease and microangiopathy, diabetes is a leading cause of foot ulcers and lower limb amputations. With the continued rise of diabetes prevalence, it is expected that the future burden of diabetes complications, early mortality, and disabilities will increase. The diabetes epidemic is partly caused by the current lack of clinical imaging diagnostic tools, the timely monitoring of insulin secretion and insulin-expressing cell mass (beta (β)-cells), and the lack of patients’ adherence to treatment, because some drugs are not tolerated or invasively administrated. In addition to this, there is a lack of efficient topical treatment capable of stopping the progression of disabilities, in particular for treating foot ulcers. In this context, polymer-based nanostructures garnered significant interest due to their tunable physicochemical characteristics, rich diversity, and biocompatibility. This review article emphasizes the last advances and discusses the prospects in the use of polymeric materials as nanocarriers for β-cell imaging and non-invasive drug delivery of insulin and antidiabetic drugs in the management of blood glucose and foot ulcers.

Published
2023
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5. Venom Peptides, Polyphenols and Alkaloids: Are They the Next Antidiabetics That Will Preserve β-Cell Mass and Function in Type 2 Diabetes?

Author

Michele Lodato, Valérie Plaisance, Valérie Pawlowski, Maxime Kwapich, Alexandre Barras, Emeline Buissart, Stéphane Dalle, Sabine Szunerits, Jérôme Vicogne, Rabah Boukherroub, and Amar Abderrahmani

Subjects
diabetes, insulin secretagogues, pancreatic beta cell, venom, polyphenols, alkaloids, Cytology, QH573-671
Abstract

Improvement of insulin secretion by pancreatic β-cells and preservation of their mass are the current challenges that future antidiabetic drugs should meet for achieving efficient and long-term glycemic control in patients with type 2 diabetes (T2D). The successful development of glucagon-like peptide 1 (GLP-1) analogues, derived from the saliva of a lizard from the Helodermatidae family, has provided the proof of concept that antidiabetic drugs directly targeting pancreatic β-cells can emerge from venomous animals. The literature reporting on the antidiabetic effects of medicinal plants suggests that they contain some promising active substances such as polyphenols and alkaloids, which could be active as insulin secretagogues and β-cell protectors. In this review, we discuss the potential of several polyphenols, alkaloids and venom peptides from snake, frogs, scorpions and cone snails. These molecules could contribute to the development of new efficient antidiabetic medicines targeting β-cells, which would tackle the progression of the disease.

Published
2023
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6. Water-Soluble Ruthenium (II) Complex Derived From Optically Pure Limonene and Its Microencapsulation Are Efficient Tools Against Bacterial Food Pathogen Biofilms: Escherichia coli, Staphylococcus aureus, Enteroccocus faecalis, and Listeria monocytogenes

Author

Simon Khelissa, Yousra El Fannassi, Samah Mechmechani, Sakhr Alhuthali, Mohamed Amin El Amrani, Adem Gharsallaoui, Alexandre Barras, and Nour-Eddine Chihib

Subjects
ruthenium (II), limonene, complexation, microencapsulation, biofilm, antibacterial agent, Microbiology, QR1-502
Abstract

Bioactive aminooxime ligands based on optically pure (R)-limonene have been synthesized in two steps. Their ruthenium (II) cationic water-soluble complex was prepared by a reaction between dichloro (para-cymene) ruthenium (II) dimers and aminooxime ligands in a 1:2 molar ratio. Antibacterial and antibiofilm activities of the synthetized complex were assessed against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis. The results revealed that the ruthenium (II) complex has higher antibacterial and antibiofilm activities in comparison with free ligands or the enantiopure (R)-limonene. Moreover, microencapsulation of this complex reduced its cytotoxicity and improved their minimum inhibitory concentration and antibiofilm activity toward the considered bacteria. The ruthenium (II) complex targets the bacterial cell membrane, which leads to rapid leakage of intracellular potassium. Our study suggests that the developed ruthenium (II) complexes could be useful as an alternative to conventional disinfectants.

Published
2021
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7. Efficient reduction of Cr(VI) under visible light irradiation using CuS nanostructures

Author

Sawsen Nezar, Yacine Cherifi, Alexandre Barras, Ahmed Addad, Elhadj Dogheche, Nadia Saoula, Nadia Aϊcha Laoufi, Pascal Roussel, Sabine Szunerits, and Rabah Boukherroub

Subjects
Chemistry, QD1-999
Abstract

Photoreduction of hexavalent chromium, Cr(VI), identified as carcinogenic and mutagenic element, to Cr(III), believed to be an essential element, using copper sulfide nanostructures (CuS NSs) as photocatalyst was investigated under visible light irradiation (λ > 420 nm). The CuS NSs were synthesized at low temperature using a wet chemical route and fully characterized using various techniques such as scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy and UV–vis spectrophotometry. The photocatalytic reduction of Cr(VI) using visible light irradiation was assessed by UV–vis by following the decrease of the characteristic absorbance at ∼352 nm in the presence of CuS NSs. While CuS NSs promoted Cr(VI) full reduction within 60 min, addition of adipic acid or formic acid accelerated significantly the photocatalytic reduction process. Under optimized conditions, CuS NSs rapidly (12 min) reduced Cr(VI) in presence of 0.5 mM adipic acid. Similarly, Cu NSs were found to be efficient for Cr(VI) reduction dissolved in water from a local lake. Stability studies showed that the photocatalyst could be recycled up to four times without any apparent decrease of its catalytic performance. Keywords: CuS nanostructures, Cr(VI), Photoreduction, Visible light, Lake water

Published
2019
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8. Enhanced Antibacterial Activity of Dermaseptin through Its Immobilization on Alginate Nanoparticles—Effects of Menthol and Lactic Acid on Its Potentialization

Author

Noura Hazime, Yanath Belguesmia, Alexandre Barras, Mohamed Amiche, Rabah Boukherroub, and Djamel Drider

Subjects
dermaseptin, DRS-B2, alginate nanoparticles, menthol, lactic acid, synergy, Therapeutics. Pharmacology, RM1-950
Abstract

Dermaseptin B2 (DRS-B2) is an antimicrobial peptide secreted by Phyllomedusa bicolor, which is an Amazonian tree frog. Here, we show that the adsorption of DRS-B2 on alginate nanoparticles (Alg NPs) results in a formulation (Alg NPs + DRS-B2) with a remarkable antibacterial activity against Escherichia coli ATCC 8739 and E. coli 184 strains, which are sensitive and resistant, respectively, to colistin. The antibacterial activity, obtained with this new formulation, is higher than that obtained with DRS-B2 alone. Of note, the addition of lactic acid or menthol to this new formulation augments its antibacterial activity against the aforementioned Gram-negative bacilli. The safety of DRS-B2, and also that of the new formulation supplemented or not with a small molecule such as lactic acid or menthol has been proven on the human erythrocytes and the eukaryotic cell line types HT29 (human) and IPEC-1 (animal). Similarly, their stability was determined under the conditions mimicking the gastrointestinal tract with different conditions: pH, temperature, and the presence of digestive enzymes. Based on all the obtained data, we assume that these new formulations are promising and could be suggested, after in vivo approval and completing regulation aspects, as alternatives to antibiotics to fight infections caused by Gram-negative bacilli such as E. coli.

Published
2022
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9. Enhancing Colistin Activity against Colistin-Resistant Escherichia coli through Combination with Alginate Nanoparticles and Small Molecules

Author

Noura Hazime, Yanath Belguesmia, Isabelle Kempf, Alexandre Barras, Djamel Drider, and Rabah Boukherroub

Subjects
alginate nanoparticles, colistin, essential oils, polyamines, lactic acid, Escherichia coli, Medicine, Pharmacy and materia medica, RS1-441
Abstract

Bacterial resistance to antibiotics has become a major public health problem worldwide, with the yearly number of deaths exceeding 700,000. To face this well-acknowledged threat, new molecules and therapeutic methods are considered. In this context, the application of nanotechnology to fight bacterial infection represents a viable approach and has experienced tremendous developments in the last decades. Escherichia coli (E. coli) is responsible for severe diarrhea, notably in the breeding sector, and especially in pig farming. The resulting infection (named colibacillosis) occurs in young piglets and could lead to important economic losses. Here, we report the design of several new formulations based on colistin loaded on alginate nanoparticles (Alg NPs) in the absence, but also in the presence, of small molecules, such as components of essential oils, polyamines, and lactic acid. These new formulations, which are made by concomitantly binding colistin and small molecules to Alg NPs, were successfully tested against E. coli 184, a strain resistant to colistin. When colistin was associated with Alg NPs, the minimal inhibition concentration (MIC) decreased from 8 to 1 µg/mL. It is notable that when menthol or lactic acid was co-loaded with colistin on Alg NPs, the MIC of colistin drastically decreased, reaching 0.31 or 0.62 µg/mL, respectively. These novel bactericidal formulations, whose innocuity towards eukaryotic HT-29 cells was established in vitro, are presumed to permeabilize the bacterial membrane and provoke the leakage of intracellular proteins. Our findings revealed the potentiating effect of the Alg NPs on colistin, but also of the small molecules mentioned above. Such ecological and economical formulations are easy to produce and could be proposed, after confirmation by in vivo and toxicology tests, as therapeutic strategies to replace fading antibiotics.

Published
2022
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10. Enhanced Antibacterial Activity of CuS-BSA/Lysozyme under Near Infrared Light Irradiation

Author

Abir Swaidan, Sena Ghayyem, Alexandre Barras, Ahmed Addad, Sabine Szunerits, and Rabah Boukherroub

Subjects
CuS-BSA NPs, lysozyme, drug delivery, NIR irradiation, photothermal agents, bacteria, Chemistry, QD1-999
Abstract

The synthesis of multifunctional photothermal nanoagents for antibiotic loading and release remains a challenging task in nanomedicine. Herein, we investigated a simple, low-cost strategy for the preparation of CuS-BSA nanoparticles (NPs) loaded with a natural enzyme, lysozyme, as an antibacterial drug model under physiological conditions. The successful development of CuS-BSA NPs was confirmed by various characterization tools such as transmission electron microscopy (TEM), X-ray diffraction (XRD), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). Lysozyme loading onto CuS-BSA NPs was evaluated by UV/vis absorption spectroscopy, Fourier-transform infrared spectroscopy (FTIR), zeta potential, and dynamic light scattering measurements. The CuS-BSA/lysozyme nanocomposite was investigated as an effective means for bacterial elimination of B. subtilis (Gram-positive) and E. coli (Gram-negative), owing to the combined photothermal heating performance of CuS-BSA and lysozyme release under 980 nm (0.7 W cm−2) illumination, which enhances the antibiotic action of the enzyme. Besides the photothermal properties, CuS-BSA/lysozyme nanocomposite possesses photodynamic activity induced by NIR illumination, which further improves its bacterial killing efficiency. The biocompatibility of CuS-BSA and CuS-BSA/Lysozyme was elicited in vitro on HeLa and U-87 MG cancer cell lines, and immortalized human hepatocyte (IHH) cell line. Considering these advantages, CuS-BSA NPs can be used as a suitable drug carrier and hold promise to overcome the limitations of traditional antibiotic therapy.

Published
2021
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11. Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds

Author

Rostyslav Bilyy, Quentin Pagneux, Nathan François, Galyna Bila, Roman Grytsko, Yuri Lebedin, Alexandre Barras, Jean Dubuisson, Sandrine Belouzard, Karin Séron, Rabah Boukherroub, and Sabine Szunerits

Subjects
coronavirus peptide, diamond nanoparticles, neutrophil extracellular traps, Medicine
Abstract

Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that the best possible antigenic components are chosen to stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based adjuvants are proposed here as a rapid and versatile platform for antigen conjugation, utilizing peptides common to different pathogenic strains and making this strategy a good candidate for a “ready-to-use” vaccine. Initiation of an inflammatory reaction with a resulting immune response is based on the ability of living organisms to entrap nanostructures such as nanodiamonds with neutrophil extracellular traps (NETs) formation. In this work, coronavirus peptide homological for MERS-CoV, fusion inhibitor, was conjugated to nanodiamonds and used to induce neutrophilic-driven self-limiting inflammation. The resulting adjuvant was safe and did not induce any tissue damage at the site of injection. Mice immunization resulted in IgG titers of ¼,000 within 28 days. Immunization of rabbits resulted in the formation of a high level of antibodies persistently present for up to 120 days after the first immunization (animal lifespan ~3 years). The peptide used for immunization proved to be reactive with sera of convalescent COVID patients, demonstrating the possibility of developing pancoronaviral vaccine candidates.

Published
2021
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12. Graphene Oxide Nanosheets for Localized Hyperthermia—Physicochemical Characterization, Biocompatibility, and Induction of Tumor Cell Death

Author

Malgorzata J. Podolska, Alexandre Barras, Christoph Alexiou, Benjamin Frey, Udo Gaipl, Rabah Boukherroub, Sabine Szunerits, Christina Janko, and Luis E. Muñoz

Subjects
graphene oxide, hyperthermia, radiotherapy, cell death, infrared, hemocompatibility, Cytology, QH573-671
Abstract

Background: The main goals of cancer treatment are not only to eradicate the tumor itself but also to elicit a specific immune response that overcomes the resistance of tumor cells against chemo- and radiotherapies. Hyperthermia was demonstrated to chemo- and radio-sensitize cancerous cells. Many reports have confirmed the immunostimulatory effect of such multi-modal routines. Methods: We evaluated the interaction of graphene oxide (GO) nanosheets; its derivatives reduced GO and PEGylated rGO, with components of peripheral blood and evaluated its thermal conductivity to induce cell death by localized hyperthermia. Results: We confirmed the sterility and biocompatibility of the graphene nanomaterials and demonstrated that hyperthermia applied alone or in the combination with radiotherapy induced much more cell death in tumor cells than irradiation alone. Cell death was confirmed by the release of lactate dehydrogenase from dead and dying tumor cells. Conclusion: Biocompatible GO and its derivatives can be successfully used in graphene-induced hyperthermia to elicit tumor cell death.

Published
2020
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13. Nanostructures for the Inhibition of Viral Infections

Author

Sabine Szunerits, Alexandre Barras, Manakamana Khanal, Quentin Pagneux, and Rabah Boukherroub

Subjects
viral infections, antiviral therapy, nanomaterials, Organic chemistry, QD241-441
Abstract

Multivalent interactions are omnipresent in biology and confer biological systems with dramatically enhanced affinities towards different receptors. Such multivalent binding interactions have lately been considered for the development of new therapeutic strategies against bacterial and viral infections. Multivalent polymers, dendrimers, and liposomes have successfully targeted pathogenic interactions. While a high synthetic effort was often needed for the development of such therapeutics, the integration of multiple ligands onto nanostructures turned to be a viable alternative. Particles modified with multiple ligands have the additional advantage of creating a high local concentration of binding molecules. This review article will summarize the different nanoparticle-based approaches currently available for the treatment of viral infections.

Published
2015
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14. Toxicity Effect of Silver Nanoparticles on Photosynthetic Pigment Content, Growth, ROS Production and Ultrastructural Changes of Microalgae Chlorella vulgaris

Author

Layla J. Hazeem, Gamze Kuku, Etienne Dewailly, Christian Slomianny, Alexandre Barras, Abderrahmane Hamdi, Rabah Boukherroub, Mustafa Culha, and Mohamed Bououdina

Subjects
silver nanoparticles, Ag ions, Chlorella vulgaris, viable cells, chlorophyll a, reactive oxygen species, Chemistry, QD1-999
Abstract

Silver nanoparticles (Ag NPs) exhibit antibacterial activity and are extensively used in numerous applications. The aim of this study was to examine the toxic effect of Ag NPs on the marine microalga, Chlorella vulgaris. The microalgae, at the exponential growth phase, were treated with different concentrations of Ag NPs (50 and 100 nm) for 96 h. X-Ray diffraction (XRD) results indicated that the used NPs are single and pure Ag phase with a mean crystallite size of 21 and 32 nm. Ag NPs were found to have a negative effect on viable cell concentration, a variable effect on chlorophyll a concentration, and increased ROS formation. Transmission electron microscopy (TEM) analysis revealed that Ag NPs were present inside the microalgae cells and formed large aggregates in the culture medium. Ag+ ions, in the form of AgNO3, were also assessed at higher concentrations and found to cause inhibitory effects.

Published
2019
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15. Exploring Light-Sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-Induced Vapor Nanobubbles

Author

Eline Teirlinck, Alexandre Barras, Jing Liu, Juan C. Fraire, Tatu Lajunen, Ranhua Xiong, Katrien Forier, Chengnan Li, Arto Urtti, Rabah Boukherroub, Sabine Szunerits, Stefaan C. De Smedt, Tom Coenye, and Kevin Braeckmans

Subjects
vapor nanobubbles, laser treatment, triggered release, liposomes, gold nanoparticles, graphene quantum dots, biofilms, diffusion barrier, Pharmacy and materia medica, RS1-441
Abstract

Impaired penetration of antibiotics through bacterial biofilms is one of the reasons for failure of antimicrobial therapy. Hindered drug diffusion is caused on the one hand by interactions with the sticky biofilm matrix and on the other hand by the fact that bacterial cells are organized in densely packed clusters of cells. Binding interactions with the biofilm matrix can be avoided by encapsulating the antibiotics into nanocarriers, while interfering with the integrity of the dense cell clusters can enhance drug transport deep into the biofilm. Vapor nanobubbles (VNB), generated from laser irradiated nanoparticles, are a recently reported effective way to loosen up the biofilm structure in order to enhance drug transport and efficacy. In the present study, we explored if the disruptive force of VNB can be used simultaneously to interfere with the biofilm structure and trigger antibiotic release from light-responsive nanocarriers. The antibiotic tobramycin was incorporated in two types of light-responsive nanocarriers—liposomes functionalized with gold nanoparticles (Lip-AuNP) and graphene quantum dots (GQD)—and their efficacy was evaluated on Pseudomonas aeruginosa biofilms. Even though the anti-biofilm efficacy of tobramycin was improved by liposomal encapsulation, electrostatic functionalization with 70 nm AuNP unfortunately resulted in premature leakage of tobramycin in a matter of hours. Laser-irradiation consequently did not further improve P. aeruginosa biofilm eradication. Adsorption of tobramycin to GQD, on the other hand, did result in a stable formulation with high encapsulation efficiency, without burst release of tobramycin from the nanocarriers. However, even though laser-induced VNB formation from GQD resulted in biofilm disruption, an enhanced anti-biofilm effect was not achieved due to tobramycin not being efficiently released from GQD. Even though this study was unsuccessful in designing suitable nanocarriers for simultaneous biofilm disruption and light-triggered release of tobramycin, it provides insights into the difficulties and challenges that need to be considered for future developments in this regard.

Published
2019
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16. Oxidative burst-dependent NETosis is implicated in the resolution of necrosis-associated sterile inflammation

Author

Mona Helena Biermann, Malgorzata Justyna Podolska, Jasmin Knopf, Christiane Reinwald, Daniela Weidner, Christian Maueröder, Jonas Hahn, Deborah Kienhöfer, Alexandre Barras, Rabah Boukherroub, Sabine Szunerits, Rostyslav Bilyy, Markus Hoffmann, Yi Zhao, Georg Schett, Martin Herrmann, and Luis Enrique Munoz

Subjects
Inflammation, Nanodiamonds, Necrosis, Resolution, reactive oxygen species (ROS), NEtosis, Immunologic diseases. Allergy, RC581-607
Abstract

Necrosis is associated with a profound inflammatory response. The regulation of necrosis-associated inflammation, particularly the mechanisms responsible for resolution of inflammation are incompletely characterized. Nanoparticles are known to induce plasma membrane damage and necrosis followed by sterile inflammation. We observed that injection of metabolically inert nanodiamonds resulted in paw edema in WT and Ncf1** mice. However, while inflammation quickly resolved in WT mice, it persisted over several weeks in Ncf1** mice indicating failure of resolution of inflammation. Mechanistically, NOX2-dependent reactive oxygen species (ROS) production and formation of neutrophil extracellular traps (NETs) were essential for the resolution of necrosis-induced inflammation: Hence, by evaluating the fate of the particles at the site of inflammation, we observed that Ncf1** mice deficient in NADPH-dependent ROS failed to generate granulation tissue therefore being unable to trap the nanodiamonds. These data suggest that NOX2-dependent NETosis is crucial for preventing the chronification of the inflammatory response to tissue necrosis by forming NETosis-dependent barriers between the necrotic and healthy surrounding tissue.

Published
2016
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17. Influence of the Size and Charge of Carbon Quantum Dots on Their Corneal Penetration and Permeation Enhancing Properties

Author

Inès De Hoon, Alexandre Barras, Tomasz Swebocki, Bernd Vanmeerhaeghe, Bram Bogaert, Cristina Muntean, Amar Abderrahmani, Rabah Boukherroub, Stefaan De Smedt, Félix Sauvage, Sabine Szunerits, Universiteit Gent = Ghent University (UGENT), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Financial support from the 'Programme Investissement d’Avenir' (I-SITE ULNE / ANR-16-IDEX-0004 ULNE) managed by the 'Agence Nationale de la Recherche' is acknowledged. In addition, this project has received funding from the European Union’s Horizon 2020 research and innovation program under the 'Marie Sklodowska-Curie grant' (agreement No 847568). Financial support from theCentre National de la Recherche Scientifique (CNRS) and the University of Lille is acknowledged in addition. F. Sauvage is a post-doctoral fellow of the Research Foundation-Flanders (grant number 12X3222N, FWO, Belgium). This work was partly supported by the French Renatech network. The authors would like to thank Maya Marinova for her help in carrying out the work on the electron microscopy facility of the Advanced Characterization Platform of the Chevreul Institute. The Chevreul Institute is thanked for help in the development of this work through the ARCHI-CM project supported by the 'Ministère de l’Enseignement Supérieur de la Recherche et de l’Innovation,' the region 'Hauts-de-France,' the ERDF program of the European Union, and the 'Métropole Européenne de Lille.', Renatech Network, ANR-16-IDEX-0004,ULNE,ULNE(2016), and European Project: 847568,H2020,H2020-MSCA-COFUND-2018,PEARL(2019)

Subjects
[SPI]Engineering Sciences [physics], endothelium, cornea, General Materials Science, fluorescence spectroscopy, epithelium, carbon quantum dots
Abstract

International audience; Reaching the corneal endothelium through the topical administration of therapeutic drugs remains a challenge in ophthalmology. Besides, endothelial cells are not able to regenerate, and diseases at this site can lead to corneal blindness. Targeting the corneal endothelium implies efficient penetration through the three corneal layers, which still remains difficult for small molecules. Carbon quantum dots (CQDs) have demonstrated great potential for ocular nanomedicine. This study focuses on the corneal penetration abilities of differently charged CQDs and their use as permeation enhancers for drugs. Excised whole bovine eyes were used as an ex vivo model to investigate corneal penetration of CQDs derived from glucosamine using β-alanine, ethylenediamine, or spermidine as a passivation agent. It was found that negatively charged CQDs have limited corneal penetration ability, while positively charged CQDs derived from glucosamine hydrochloride and spermidine (CQD-S) penetrate the entire corneal epithelium all the way down to the endothelium. CQD-S were shown to enhance the penetration of FITC-dextran 150 kDa, suggesting that they could be used as efficient penetration enhancers for therapeutic delivery to the corneal endothelium.

Published
2023
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18. Interface Engineering of an Ultrathin Hierarchical Phosphorus-Doped MoS2/Ni3S2 Heterostructure on Nickel Foam for Efficient Water Splitting

Author

Yuanyuan Miao, Saifei Yuan, Hao Ren, Ahmed Addad, Alexandre Barras, Pascal Roussel, Mohammed A. Amin, Sabine Szunerits, Rabah Boukherroub, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), China University of Petroleum, Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Taif University (TU), Partenaires INRAE, and ICP analyses were performed in the ≪ Spectrométrie par torche à plasma ≫ platform of the Research Federation Michel-Eugène Chevreul hosted by the LASIRE laboratory. The authors acknowledge the financial support from the Centre National de la Recherche Scientifique (CNRS), the University of Lille, and the Hauts-de-France region. Y.M. acknowledges the Chinese government for the China Scholarship Council fellowship. The authors acknowledge Taif University Researchers Supporting Project number TURSP-2020/03, Taif University, Taif, Saudi Arabia, for financial support of this project.

Subjects
hydrogen evolution, oxygen evolution, [PHYS]Physics [physics], [SPI]Engineering Sciences [physics], phosphorus doping, MoS2/Ni3S2, high stability, Materials Chemistry, Electrochemistry, Energy Engineering and Power Technology, Chemical Engineering (miscellaneous), Electrical and Electronic Engineering, water splitting
Abstract

International audience; Rationally designed structures and elements are important for the construction of effective electrocatalysts for water splitting. Herein, we developed an ultrathin hierarchical P-doped MoS2/Ni3S2 heterostructure on nickel foam (NF) by a one-pot synthesis. Owing to its optimized electronic structure configuration and hierarchical structure with plenty of active sites, the optimized catalyst P1.0-doped MoS2/Ni3S2/NF showed promising activity for both hydrogen evolution reaction and oxygen evolution reaction in an alkaline medium requiring an overpotential of, respectively, 0.449 and 0.239 V at 100 mA cm–2. Furthermore, the P1.0–MoS2/Ni3S2/NF electrode was applied for overall water splitting using a two-electrode system. The electrolyzer achieved low cell voltages of 1.50 and 1.65 V at current densities of, respectively, 20 and 40 mA cm–2 with excellent stability over 60 h when compared with previously reported Ni3S2-based materials. The results obtained in this work highlight the prime combination of doping and interface engineering for the design of advanced materials in electrocatalysis.

Published
2023
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19. Colorimetric assay for the detection of dopamine using bismuth ferrite oxide (Bi2Fe4O9) nanoparticles as an efficient peroxidase-mimic nanozyme

Author

Mehri Razavi, Alexandre Barras, Madjid Ifires, Abir Swaidan, Maryam Khoshkam, Sabine Szunerits, Mohsen Kompany-Zareh, Rabah Boukherroub, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Dalhousie University [Halifax], and The authors wish to thank the Centre National de la Recherche Scientifique (CNRS), the University of Lille, the region Hauts-de-France, the CPER 'Photonics for Society', and the Institute for Advanced Studies in Basic Sciences (IASBS) in Iran for supporting this study.

Subjects
Biomaterials, [SPI]Engineering Sciences [physics], Colloid and Surface Chemistry, [CHIM]Chemical Sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials
Abstract

International audience; This work describes the preparation of ternary bismuth ferrite oxide nanoparticles (Bi2Fe4O9 NPs) with an enzyme mimetic activity for dopamine (DA) qualitative and quantitative detection. Bi2Fe4O9 NPs were prepared using a facile, low cost, and one-pot hydrothermal treatment. The chemical composition, morphology, and optical properties of Bi2Fe4O9 nanozyme were characterized using different techniques such as Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction pattern (XRD), X-ray photoelectron spectroscopy (XPS), thermo-gravimetric analysis (TGA), dynamic light scattering (DLS), field-emission scanning electron microscopy (FESEM) imaging, FESEM-energy dispersive X-ray spectroscopy (EDS), UV–vis absorption, and fluorescence emission spectroscopy. Bi2Fe4O9 NPs were utilized to catalyze the oxidation of a typical chromogenic peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine (TMB), to form the blue-colored oxidized product (oxTMB), in the presence of hydrogen peroxide (H2O2). All reactions occurred in acetate buffer solution (pH 3.5) to generate hydroxyl radicals (•OH) and the kinetics were followed by UV–vis absorbance at 654 nm. The steady-state kinetic parameters were obtained from the Michaelis-Menten equation and exhibited a good catalytic efficiency of Bi2Fe4O9 NPs as enzyme mimetics. Michaelis–Menten constant (Km) values were estimated as 0.07 and 0.73 mM for TMB and H2O2, respectively. The presented method is efficient, rapid, cost-effective, and sensitive for the colorimetric detection of dopamine with a linear range (LR) from 0.15 to 50 μM and a detection limit (LOD) of 51 nM. The proposed colorimetric sensor was successfully applied for the detection of different concentrations of dopamine in spiked fetal bovine serum (FBS) and horse serum (HS) samples. It is anticipated that Bi2Fe4O9 nanozyme holds great potential in biomedical analysis and diagnostic applications of dopamine-related diseases.

Published
2022
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20. [Review] Deep Eutectic Solvents Comprising Organic Acids and Their Application in (Bio)Medicine

Author

Tomasz Swebocki, Alexandre Barras, Amar Abderrahmani, Kamel Haddadi, Rabah Boukherroub, University of Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], NanoBioInterfaces - IEMN [NBI - IEMN], Circuits Systèmes Applications des Micro-ondes - IEMN [CSAM - IEMN ], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Circuits Systèmes Applications des Micro-ondes - IEMN (CSAM - IEMN ), Funding: The doctorate of TS is financed by the French Ministry of Higher Education, Research,and Innovation (MESRI). The CNRS, the University of Lille and the Hauts-de-France region areacknowledged for financial support., Acknowledgments: Figure 4 and the graphical abstract have been created using Biorender® softwareassets. The CNRS, the University of Lille, the Hauts-de-France region, and the Agence Nationale dela Recherche (project: MSN-2hv) are acknowledged for financial support., and ANR-19-CE09-0034,MSN-2hv,Matériaux théranostiques associant les fonctionnalités d'imagerie 2 photons et de thérapie photodynamique pour le ciblage de bactéries et le contrôle des infections lors de la cicatrisation(2019)

Subjects
Organic Chemistry, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, General Medicine, Catalysis, Computer Science Applications, Inorganic Chemistry, organic acids, drug delivery, enhancers, antimicrobial treatment, Physical and Theoretical Chemistry, Molecular Biology, deep eutectic solvents, Spectroscopy
Abstract

International audience; Over the last years, we observed a significant increase in the number of published studies that focus on the synthesis and characterization of deep eutectic solvents (DESs). These materials are of particular interest mainly due to their physical and chemical stability, low vapor pressure, ease of synthesis, and the possibility of tailoring their properties through dilution or change of the ratio of parent substances (PS). DESs, considered as one of the greenest families of solvents, are used in many fields, such as organic synthesis, (bio)catalysis, electrochemistry, and (bio)medicine. DESs applications have already been reported in various review articles. However, these reports mainly described these components’ basics and general properties without focusing on the particular, PS-wise, group of DESs. Many DESs investigated for potential (bio)medical applications comprise organic acids. However, due to the different aims of the reported studies, many of these substances have not yet been investigated thoroughly, which makes it challenging for the field to move forward. Herein, we propose distinguishing DESs comprising organic acids (OA-DESs) as a specific group derived from natural deep eutectic solvents (NADESs). This review aims to highlight and compare the applications of OA-DESs as antimicrobial agents and drug delivery enhancers—two essential fields in (bio)medical studies where DESs have already been implemented and proven their potential. From the survey of the literature data, it is evident that OA-DESs represent an excellent type of DESs for specific biomedical applications, owing to their negligible cytotoxicity, fulfilling the rules of green chemistry and being generally effective as drug delivery enhancers and antimicrobial agents. The main focus is on the most intriguing examples and (where possible) application-based comparison of particular groups of OA-DESs. This should highlight the importance of OA-DESs and give valuable clues on the direction the field can take.

Published
2023
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21. Preparation of Flower-Shaped Co-Fe Layer Double Hydroxide Nanosheets Loaded with Pt Nanoparticles by Corrosion Engineering for Efficient Electrocatalytic Water Splitting

Author

Zhao-Hui Zhang, Yi Zhang, Alexandre Barras, Ahmed Addad, Pascal Roussel, Long-Cheng Tang, Mohammed A. Amin, Sabine Szunerits, Rabah Boukherroub, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Hangzhou Normal University, Taif University (TU), Partenaires INRAE, and The authors thank the Centre National de la Recherche Scientifique (CNRS), the University of Lille, the Hauts-de-France region, and the CPER 'WaveTech' for financial support. Z.-H.Z. thanks the Chinese government for the China Scholarship Council (CSC) fellowship. The authors thank the ICP analyses platform of the Research Federation Michel-Eugène Chevreul hosted by the LASIRE laboratory. The authors are also thankful to the Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, Saudi Arabia.

Subjects
[PHYS]Physics [physics], [SPI]Engineering Sciences [physics], iron foam, oxygen evolution reaction, oxygen corrosion technology, Materials Chemistry, Electrochemistry, Energy Engineering and Power Technology, Chemical Engineering (miscellaneous), Electrical and Electronic Engineering, Pt nanoparticles, Co/Fe LDH, hydrogen evolution reaction
Abstract

International audience; The development of high-performance and cost-effective hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) electrocatalysts is highly required for electrochemical water splitting. The sluggish reaction kinetics, poor stability, and complicated processing restrict the practical application of most electrocatalysts. Herein, we report a facile oxygen corrosion strategy for the fabrication of Pt nanoparticles loaded on cobalt-iron layer double hydroxide (Pt-Co/Fe LDH) on iron foam using an oxygen corrosion method regulated by sodium chloride (NaCl). The as-prepared Pt-Co/Fe LDH electrodes feature overpotentials as low as 126 and 285 mV to deliver 100 mA cm–2 for HER and OER, respectively. Moreover, the Pt-Co/Fe LDH was applied as anode and cathode in an electrochemical electrolyzer and recorded a current density of 50 mA cm–2 at a small voltage of 1.66 V, which is superior to that of the Pt/C-FF || RuO2-FF system (1.89 V) and those of most previously reported electrocatalysts. The excellent electrocatalytic activity of Pt-Co/Fe LDH could be assigned to its specific structure, consisting of three-dimensional (3D) nanoflower-shaped nanosheets, favorable for promoting fast mass transport and ion diffusion and generating more active sites for the OER process. In addition, the synergistic effect between Pt and the Co/Fe LDH heterojunction structure effectively improves the electronic conductivity and H adsorption for HER performance. The oxygen corrosion method regulated by NaCl and interface engineering of the LDH structure is simple to implement and can be easily extended for the preparation of a plethora of other bifunctional and cost-effective electrocatalysts for various electrochemical processes.

Published
2022
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22. A colorimetric assay and MCR-ALS analysis of the peroxidase-like activity of poly (N-phenylglycine) functionalized with polyethylene glycol (PNPG-PEG) nanozyme for the determination of dopamine

Author

Mehri Razavi, Alexandre Barras, Sabine Szunerits, Maryam Khoshkam, Mohsen Kompany-Zareh, Rabah Boukherroub, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Institute for Advanced Studies in Basic Sciences (IASBS), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), University of Mohaghegh Ardabili, Dalhousie University [Halifax], and The authors gratefully acknowledge the financial support provided by the Centre National de la Recherche Scientifique (CNRS), the University of Lille, the region Hauts-de-France, the CPER ‘‘Photonics for Society', and the Institute for Advanced Studies in Basic Sciences (IASBS) in Iran for supporting this work.

Subjects
Serum, [PHYS]Physics [physics], Dopamine, Glycine, Hydrogen Peroxide, Biochemistry, Polyethylene Glycols, Analytical Chemistry, [SPI]Engineering Sciences [physics], Peroxidases, PNPG-PEG, Peroxidase mimics, Animals, Environmental Chemistry, Colorimetry, Horses, Spectroscopy, Colorimetric detection, Peroxidase
Abstract

International audience; This report describes, for the first time, the coupling of UV–visible spectroscopy with multivariate curve resolution-alternative least-squares (MCR-ALS) algorithm to study peroxidase-like catalytic reaction of polyethylene glycol-functionalized poly (N-phenyl glycine) (PNPG-PEG) as an efficient and intrinsic peroxidase mimic activity (PMA) class of conducting organic polymer for selective detection of dopamine (DA) in the PNPG-PEG + TMB + H2O2 reaction system. PNPG-PEG was produced by means of a chemical route using ammonium persulphate (APS) as an oxidant agent of N-phenyl glycine monomer. The chemical composition, morphology, and thermal behavior of PNPG-PEG were examined by various instrumental techniques. PNPG-PEG exhibited significant peroxidase-mimic activity to catalyze the oxidation 3,3′,5,5′- tetramethylbenzidine (TMB) substrate to oxidized TMB (oxTMB). The qualitative and quantitative determination of the oxidized TMB can easily be detected by the naked-eye and the recorded UV–vis absorbance spectra at 652 nm, respectively. Owing to the superior peroxidase-mimic activity of PNPG-PEG, the colorimetric detection of dopamine was successfully achieved at pH 4.0. Under optimal conditions, acceptable linear dependency was recorded in the concentration range of 5.1–125.0 μM, with a limit of detection (LOD) and limit of quantification (LOQ) equal to 4.6 μM and 13.8 μM (S/N = 3), respectively. Furthermore, this colorimetric assay was successfully used for quantitative analysis of dopamine in fetal bovine serum (FBS) and horse serum (HS) samples with recoveries in the range of 97–105% and 100–122%, respectively. After resolving the bilinear data matrix using MCR-ALS, three chemical components were found for different concentrations and pure spectral profiles. Based on the resolved profiles, the presence of free, slightly penetrated, and majorly penetrated TMB molecules entering the polymeric structure can be easily detected using MCR-ALS as an available statical method without any complex separation instruments. This peroxidase mimetic nanozyme as a visual, simple, low-cost, sensitive, and reproducible colorimetric platform can provide great potential applications in the monitoring and diagnosis of dopamine-related diseases.

Published
2022
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23. A Gamificação no Ensino da Geografia: Teoria e Aplicações

Author

Baptista, Alexandre Barras, Fiel, Elisabete Maria Bailadeira Amiguinho, and Julião, Rui Pedro de Sousa Pereira Monteiro

Subjects
Ensino, Ciências Sociais::Ciências da Educação [Domínio/Área Científica], Motivação, Motivation, Gamificação, Jogo, Geography, Ciências Sociais::Geografia Económica e Social [Domínio/Área Científica], Teaching, Game, Gamification, Geografia
Abstract

Num período onde a transição digital é evidente em várias áreas do conhecimento, é imprescindível que o ensino acompanhe esta evolução e que implemente, num ambiente controlado, as ferramentas tecnológicas que contribuam para aumentar o interesse dos alunos e também a consolidação das suas aprendizagens. Uma dessas ferramentas é justamente a gamificação. A implementação de gamificação em sala de aula encontra-se numa fase de pleno desenvolvimento, mas é alvo de muita resistência devido ao seu carácter lúdico e por vezes exigente a nível técnico. No entanto, são vários os exemplos onde o seu uso resulta num maior interesse e motivação por parte dos alunos. Apesar de existirem exemplos de aplicabilidade, especialmente no caso da matemática, são ainda raros ou pouco estruturados os exemplos na disciplina de geografia. Este trabalho, através da metodologia adotada na Prática de Ensino Supervisionada pretende demonstrar que a implementação de jogos digitais pode constituir um recurso útil na aprendizagem dos alunos e na sua motivação, através de uma solução harmoniosa enquadrando a componente didática com os conteúdos programáticos. Para além do desenvolvimento de jogos, foi implementado um questionário aos alunos onde eram encorajados a avaliar e a providenciar feedback quanto à utilização de gamificação ao longo do ano letivo como também a sugerir metodologias e aplicações passíveis de serem aplicadas. In a period where the digital transition is clear in several areas of knowledge, it is essential that teaching keeps up with this evolution and implements, in a controlled environment, technological tools that contribute to increase student interest and the consolidation of their learning. One of these tools is precisely gamification. The implementation of gamification in the classroom is in a stage of full development but is subject to much resistance due to its playful and sometimes technically demanding nature. However, there are several examples where its use results in increased student interest and motivation. Although there are examples of applicability, especially in the case of mathematics, examples in geography are still rare or poorly structured. This work, through the methodology adopted in the Supervised Teaching Practice, aims to demonstrate that the implementation of digital games can be a useful resource in student learning and motivation, through a harmonious solution framing the didactic component with the syllabus. In addition to the development of games, a questionnaire was given to students to encourage them to evaluate and provide feedback on the use of gamification throughout the school year, and to suggest methodologies and applications that could be applied.

Published
2022

24. Anti-aggregation effect of carbon quantum dots on diabetogenic and beta-cell cytotoxic amylin and beta amyloid heterocomplexes

Author

Anna Voronova, Alexandre Barras, Valérie Plaisance, Valerie Pawlowski, Rabah Boukherroub, Amar Abderrahmani, Sabine Szunerits, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), AV thanks the I-SITE University of Lille Foundation for a PhD fellowship. Financial support from the CNRS and ULille is acknowledged. This work was partly supported by the French Renatech network., Renatech Network, and CMNF

Subjects
Amyloid, Amyloid beta-Peptides, Fatty Acids, Palmitates, Amyloidogenic Proteins, Carbon, Islet Amyloid Polypeptide, Protein Aggregates, [SPI]Engineering Sciences [physics], Glucose, Diabetes Mellitus, Type 2, Quantum Dots, Humans, General Materials Science
Abstract

International audience; Pancreatic islet amyloid deposition is a pathological hallmark of Type 2 diabetes (T2D), contributing to reduced functional β-cell mass. Islet amyloids result not only from the aggregation and fibrillation of human islet amyloid polypeptide (hIAPP), but also from beta-amyloid 42 (Aβ42), the key amyloidogenic peptide linked to Alzheimer's disease. Importantly, Aβ42 and hIAPP aggregates (IAPP:Aβ42) can interact with each other and form some harmful heterocomplex fibrils. While it is well-documented that hIAPP aggregation occurs only when islets are exposed to a diabetic environment, including hyperglycemia and/or elevated concentrations of saturated fatty acids (SFAs), it remains unclear if hIAPP and IAPP:Aβ42 heteromer fibrillations are directly or indirectly triggered by this environment. In this study, we show the interplay between high glucose concentrations and palmitate as the SFA in the aggregation of hIAPP. In addition, we outline that the interaction of hIAPP and Aβ42 leads to the formation of complex protein aggregates, which are toxic to β-cells. Carbon nanocolloids in the form of positively charged carbon quantum dots (CQD-pos) efficiently prevent single amyloid aggregation and the formation of IAPP:Aβ42 heterocomplexes. We provide clear evidence with this study that the diabetogenic environment of islets could directly contribute to the formation of homomeric and heteromeric amyloid aggregates and fibrils in T2D. We also propose carbon nanocolloids as biocompatible nanomaterials for developing innovative therapeutic strategies that prevent the decline of functional β-cell mass.

Published
2022
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25. Discovery of a Peptide Nucleic Acid (PNA) aptamer for cardiac troponin I: Substituting DNA with neutral PNA maintains picomolar affinity and improves performances for electronic sensing with graphene field-effect transistors (gFET)

Author

Teresa Rodrigues, Federica Curti, Yann R. Leroux, Alexandre Barras, Quentin Pagneux, Henri Happy, Christoph Kleber, Rabah Boukherroub, Roger Hasler, Stefano Volpi, Maria Careri, Roberto Corradini, Sabine Szunerits, Wolfgang Knoll, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Austrian Institute of Technology [Vienna] (AIT), Università degli studi di Parma = University of Parma (UNIPR), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Carbon - IEMN (CARBON - IEMN), Dipartimento di Scienze Chimiche,della Vita e della Sostenibilità Ambientale, Danube Private University [Krems, Autriche] (DPU), and Financial supports from the Centre National de la Recherche Scientifique (CNRS), the University of Lille are acknowledged. This work has benefited from the equipment and framework of the COMP-HUB Initiative, funded by the ‘Departments of Excellence’ program of the Italian Ministry for University and Research (MUR, 2018–2022) for the Department of Chemistry, Life Sciences and Environmental Sustainability of the University of Parma. The TeachInParma Program is also acknowledged for sponsoring supervision and collaborative work of WK at the University of Parma premises

Subjects
Graphene-based field effect transistor, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Aptamer, Peptide nucleic acid, Cardiac troponin I, Biomedical Engineering, Pharmaceutical Science, General Materials Science, Bioengineering, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Biosensor, Biotechnology
Abstract

International audience; DNA-based aptamers are widely employed as bioreceptors, owing to their tuneable affinity and specificity towards their targets. The use of peptide nucleic acids (PNAs) has instead proven challenging for this purpose, due to the absence of selection methods for the independent discovery of suitable receptors and due to the difficult mimicry of established DNA-based ones. Despite that PNAs exceed homologous DNA or RNA in terms of complementary base pairing, they can fail to reproduce alternative modes of binding because of their different structural features. The remarkable stability and charge distribution of PNAs could be beneficial to produce sensing bioreceptors, especially in the development of electronic devices such as field-effect transistor-based (FET) biosensors. We hereby report for the first time a high-affinity PNA aptamer for cardiac Troponin I (cTnI), a biomarker of acute myocardial infarction, able to interact with this specific protein in the picomolar range. The PNA aptamer was immobilized onto a graphene-based FET (gFET) transducer, and its ability in the direct detection of cTnI was compared with that of a DNA-based one of the same sequence. Similar dissociation constants were recorded for both receptors in 0.01 × PBS, as well as comparable detection limits of 6.0 ± 1.0 pg mL-1 (PNA aptamer) and 3.3 ± 0.7 pg mL-1 (DNA aptamer). Apart from the non-trivial demonstration that a PNA can behave as an aptamer, the tested receptor proved to be more consistent upon working in more complex biological matrices.

Published
2023
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26. TRPM8 as an Anti-Tumoral Target in Prostate Cancer Growth and Metastasis Dissemination

Author

Guillaume P. Grolez, Giorgia Chinigò, Alexandre Barras, Mehdi Hammadi, Lucile Noyer, Kateryna Kondratska, Etmar Bulk, Thibauld Oullier, Séverine Marionneau-Lambot, Marilyne Le Mée, Stéphanie Rétif, Stéphanie Lerondel, Antonino Bongiovanni, Tullio Genova, Sébastien Roger, Rabah Boukherroub, Albrecht Schwab, Alessandra Fiorio Pla, Dimitra Gkika, Laboratoire de Physiologie Cellulaire : Canaux ioniques, inflammation et cancer - U 1003 (PHYCELL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Neuro-Dol (Neuro-Dol), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate, Université de Lille, Sciences et Technologies-Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli studi di Torino = University of Turin (UNITO), NanoBioInterfaces - IEMN (NBI - IEMN), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Westfälische Wilhelms-Universität Münster = University of Münster (WWU), Cancéropôle Grand-Ouest [Bretagne-Centre-Pays de Loire], Centre d'Imagerie du Petit Animal (CIPA), Université d'Orléans (UO), Transgenèse et archivage d'animaux modèles (TAAM), Centre National de la Recherche Scientifique (CNRS), CHU Lille, Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 (PLBS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), EA4245 - Transplantation, Immunologie, Inflammation [Tours] (T2i), Université de Tours (UT), Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 (CANTHER), This research was funded by the Ministère de l’Education Nationale and the InstitutNational de la Santé et de la Recherche Medicale (INSERM). The research of G.P.G. was supported bythe region Hauts–de–France and the University of Lille. The research of D.G. was supported by theInstitut Universitaire de France (IUF) and France Berkley Fund. D.G., A.F.P. and R.B. were supportedby the Institut National du Cancer (INCa—PLBIO14–213) and the CPER 'Photonics for Society'.A.F.P. and G.C. are supported by the Italian Ministry of Instruction, University and Research (MIUR),PRIN grant 'Leveraging basic knowledge of ion channel network in cancer for innovative therapeuticstrategies (LIONESS)' (grant number 20174TB8KW) and are part of the Associated InternationalLaboratory (CaPANCInv). A.S. is part of the Associated International Laboratory (CaPANCInv) andis supported by Deutsche Forschungsgemeinschaft SCHW 407/22-1., and IEMN, Collection

Subjects
TRPM8, Male, Carcinogenesis, [SDV]Life Sciences [q-bio], TRPM Cation Channels, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, Catalysis, Cell Line, Inorganic Chemistry, metastasis dissemination, Mice, [SDV.CAN] Life Sciences [q-bio]/Cancer, Cell Movement, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Cell Line, Tumor, Animals, Humans, Physical and Theoretical Chemistry, Neoplasm Metastasis, Molecular Biology, Spectroscopy, Cell Proliferation, Tumor, FAK, ERK, Rho signaling, cell proliferation, invasion, prostate cancer, trans–endothelial migration, tumor growth, Membrane Proteins, Prostate, Prostatic Neoplasms, Organic Chemistry, General Medicine, [SDV.MHEP.UN] Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, Computer Science Applications, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Abstract

International audience; In the fight against prostate cancer (PCa), TRPM8 is one of the most promising clinical targets. Indeed, several studies have highlighted that TRPM8 involvement is key in PCa progression because of its impact on cell proliferation, viability, and migration. However, data from the literature are somewhat contradictory regarding the precise role of TRPM8 in prostatic carcinogenesis and are mostly based on in vitro studies. The purpose of this study was to clarify the role played by TRPM8 in PCa progression. We used a prostate orthotopic xenograft mouse model to show that TRPM8 overexpression dramatically limited tumor growth and metastasis dissemination in vivo. Mechanistically, our in vitro data revealed that TRPM8 inhibited tumor growth by affecting the cell proliferation and clonogenic properties of PCa cells. Moreover, TRPM8 impacted metastatic dissemination mainly by impairing cytoskeleton dynamics and focal adhesion formation through the inhibition of the Cdc42, Rac1, ERK, and FAK pathways. Lastly, we proved the in vivo efficiency of a new tool based on lipid nanocapsules containing WS12 in limiting the TRPM8–positive cells’ dissemination at metastatic sites. Our work strongly supports the protective role of TRPM8 on PCa progression, providing new insights into the potential application of TRPM8 as a therapeutic target in PCa treatment.

Published
2022
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27. Highly performing graphene-based field effect transistor for the differentiation between mild-moderate-severe myocardial injury

Author

Teresa Rodrigues, Vladyslav Mishyn, Yann R. Leroux, Laura Butruille, Eloise Woitrain, Alexandre Barras, Patrik Aspermair, Henri Happy, Christoph Kleber, Rabah Boukherroub, David Montaigne, Wolfgang Knoll, Sabine Szunerits, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Austrian Institute of Technology [Vienna] (AIT), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 (EGID), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Carbon - IEMN (CARBON - IEMN), Financial support from the Centre National de la Recherche Scientifique (CNRS), the University of Lille, CPER 'Photonics for Society' and I-Site is acknowledged. DM, LB and EW are supported by grants from Agence Nationale pour la Recherche (ANR-10-LABX-0046, ANR TOMIS-Leukocyte: ANR-CE14–0003-01 and ANR CALMOS: ANR-18-CE17–0003-02), the Leducq Foundation LEAN Network 16CVD01 and the National Center for Precision Diabetic Medicine–PreciDIAB (ANR-18-IBHU-0001, 20001891/NP0025517, 2019_ESR_11)., ANR-10-LABX-0046,EGID,EGID Diabetes Pole(2010), ANR-19-CE14-0003,TOMIS-leukocyte,Impact du moment de la journée sur la réaction inflammatoire péri-opératoire et le remodelage cardiaque inverse après remplacement valvulaire aortique(2019), ANR-18-CE17-0003,CALMOS,Homéostasie calcique mitochondriale dans le contrôle des troubles du rythme associés à la cardiomyopathie métabolique(2018), and ANR-18-IBHU-0001,PreciDIAB,PreciDIAB Institute, the holistic approach of personal diabets care(2018)

Subjects
Graphene-based field effect transistor, cardiac troponin I, Biomedical Engineering, Pharmaceutical Science, aptamer, General Materials Science, Bioengineering, macromolecular substances, [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph], musculoskeletal system, biosensor, Biotechnology
Abstract

International audience; Cardiovascular diseases result in millions of deaths around the globe, many of which could have been avoided if identified at an early stage. Preventive cardiovascular disease diagnostics plays a vital role reducing the critical fatality rate by allowing to take timely necessary precautions. Here, we demonstrate the exceptional properties of aptamer modified graphenebased FET (GFET) for cardiac troponin I (cTnI) specific sensing in different bodily fluids. Concentration-dependent measurements made with cardiac troponin I (cTnI) on an aptamer/polyethylene glycol (PEG) modified GFET exhibited real-time detection of cTnI from 1-400 pg mL-1 in 0.01× phosphate-buffered saline (PBS) solution. The sensor performance is within the clinical important window of 10 pg mL-1 to 500 pg mL-1 , allowing the differentiation between healthy, and people with low and high risk for myocardial infraction (AMI). Even more important is the very reproducible shift and neglectable leakage current of the individual IDSVGS-curves upon contact with cTnI solutions. To further evaluate the applicability of the developed point of care device for troponin I sensing in serum, samples of 15 patients with different troponin levels were analysed for their troponin I levels. The 15 patients were grouped

Published
2022
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28. Phytic acid-doped poly-N-phenylglycine potato peels for removal of anionic dyes: investigation of adsorption parameters

Author

Kahina Bouhadjra, Alexandre Barras, Wahiba Lemlikchi, Ahmed Addad, Manash R. Das, Mohammed A. Amin, Sabine Szunerits, Rabah Boukherroub, Université Mouloud Mammeri [Tizi Ouzou] (UMMTO), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Academy of Scientific and Innovative Research [Ghaziabad, India] (AcSIR), Partenaires INRAE, Taif University (TU), and The Centre National de la Recherche Scientifique (CNRS), the University of Lille, and the Hauts-de-France region are acknowledged for financial support. The authors also thank the Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, Saudi Arabia.

Subjects
[SPI]Engineering Sciences [physics], Materials Chemistry, General Chemistry, Catalysis
Abstract

International audience; In this work, we report on enhanced adsorption of anionic dyes, Reactive blue 49 (RB 49) and Direct Blue 199 (DB 199), using a phytic acid-doped poly-N-phenylglycine@potato peel (PA-PPG@PP) nanocomposite material. The PA-PPG@PP was prepared using a radical polymerization process by varying the amount of potato peel powder (PP) in the composite material. The surface structure and composition were examined by zeta potential, scanning electron microscopy (SEM), Fourier-transform infrared (FTIR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and Brunauer–Emmett–Teller (BET) analysis. The influence of different operating conditions such as adsorbent dose, initial dye concentration, contact time, pH and ionic strength on the removal efficiency of PA-PPG@PP2 was assessed to achieve the optimum conditions for RB 49 dye adsorption. Adsorption equilibrium data were well-described by the Langmuir model and the maximum adsorption capacities of PA-PPG@PP2 for RB 49 and DB 199 at 298 K and pH = 6.5 were 216 and 1148 mg g−1, respectively. The maximum adsorption capacity of RB 49 dye on the PA-PPG@PP2 surface was achieved at 318 K (285 mg g−1). The adsorption process was described by the pseudo-second-order kinetics model (R2 > 0.999). The thermodynamic study proved that the adsorption processes were endothermic and favorable. The dye molecules were adsorbed on the composite material surface mainly through hydrophobic and electrostatic interactions, as well as hydrogen bonding. Additionally, our experimental results suggested that the hydrophobic interactions increased upon increasing ionic strength, while the electrostatic interactions decreased upon elevating the ionic strength of the solution. The findings in this work revealed the potential of biomass powder, potato peels, in adsorption processes upon hybridization with a biocompatible organic polymer, poly-N-phenylglycine.

Published
2022
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29. Effective PDT/PTT dual-modal phototherapeutic killing of bacteria by using poly(N-phenylglycine) nanoparticles

Author

Sena Ghayyem, Alexandre Barras, Farnoush Faridbod, Sabine Szunerits, Rabah Boukherroub, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), University of Tehran, NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), This study is financially supported by the Centre National de la Recherche Scientifique (CNRS), the University of Lille, the Hauts-de-France region, and the CPER 'Photonics 4 Society' for financial support. This work was partly supported by the French Renatech network., and Renatech Network

Subjects
Staphylococcus aureus, [SPI]Engineering Sciences [physics], Photochemotherapy, Escherichia coli, Glycine, Nanoparticles, Triazenes, ComputingMilieux_MISCELLANEOUS, Anti-Bacterial Agents, Analytical Chemistry
Abstract

International audience; This study investigated, for the first time, the antimicrobial properties of polyethylene glycol-functionalized poly(N-phenylglycine) nanoparticles (PNPG-PEG NPs). PNPG-PEG NPs exhibit high extinction coefficient in the near-infrared (NIR) region; they can convert light energy into heat energy with high thermal transformation efficiency. Additionally, they can generate cytotoxic reactive oxygen species (ROS) upon light irradiation. Also, PNPG-PEG NPs are not cytotoxic. All these properties make them appropriate for combined dual-modal photothermal and photodynamic therapies. The antibacterial activity of PNPG-PEG NPs was assessed using Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) pathogenic strains. The results revealed that NIR light (810 nm) irradiation for 10 min could kill effectively the planktonic bacteria and destroy Escherichia coli and Staphylococcus aureus biofilms. The results demonstrated that PNPG-PEG NPs represent a very effective nanoplatform for killing of pathogenic bacteria.

Published
2022
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30. Photothermal Activatable Mucoadhesive Fiber Mats for On-Demand Delivery of Insulin via Buccal and Corneal Mucosa

Author

Anna Voronova, Cristina Prieto, Maria Pardo-Figuerez, Jose Maria Lagaron, Amitav Sanyal, Bilal Demir, Thomas Hubert, Valerie Plaisance, Valerie Pawlowski, Séverine Vignoud-Despond, Alexandre Barras, Amar Abderrahmani, Rabah Boukherroub, Sabine Szunerits, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), European Union's Horizon 2020 Research and Innovation Staff Exchange (RISE) Marie Skłodowska-Curie Actions under grant agreement No 690836., European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Centre National de la Recherche Scientifique (France), Université de Lille, and European commission

Subjects
Swine, Biomedical Engineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, reduced graphene oxide, Biomaterials, Cornea, [SPI]Engineering Sciences [physics], on-demand release, Insulin, Regular, Human, Animals, Humans, Insulin, [CHIM]Chemical Sciences, mucoadhesive buccal mucosa, Ensure healthy lives and promote well-being for all at all ages, electrospinning, Biochemistry (medical), Mouth Mucosa, Administration, Buccal, Hydrogels, General Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, 3. Good health, Buccal mucosa, Mucoadhesive, 0210 nano-technology
Abstract

Electrospun fiber mats loaded with therapeutics have gained considerable attention as a versatile tool in the biomedical field. While these bandages are largely based on fast-dissolving polymers to release the cargo, stimuli-responsive fiber mats have the advantages of providing a timely and spatially controlled drug delivery platform, which can be refilled and reused several times. These benefits make electrospun fiber patches original platforms for painless and convenient on-demand hormone release. Because of the high need of more convenient and non-invasive methods for delivering insulin, a hormone that is currently used to treat hundred million people with diabetes worldwide, we have investigated the tremendous potential of reduced graphene oxide modified poly(acrylic acid) based fiber mats as an original platform for buccal and corneal insulin delivery on-demand. The PAA@rGO hydrogel-like fibers rendered water-insoluble by incorporating β-cyclodextrin, followed by thermal cross-linking, which showed adequate tensile strength along with high adsorption capacity of insulin at pH 7 and good recyclability. The fiber mats maintained good fibrous morphology and high loading efficiency even after five loading-release cycles. The mucoadhesive nature of the fibers allowed their application for insulin delivery via the eye cornea and the buccal mouth lining, as evidenced in ex vivo studies. Insulin loaded PAA@rGO hydrogel-like fibers showed an insulin flux via buccal lining of pigs of 16.6 ± 2.9 μg cm-2 h-1 and 24.3 ± 3.1 μg cm-2 h-1 for porcine cornea. Testing on healthy adult volunteers confirmed the excellent, mucoadhesive nature of the bandage, with three out of six volunteers feeling completely comfortable (note 8.3) while wearing the patches in the buccal cavity., The Centre National de la Recherche Scientifique (CNRS), the University of Lille, the Hauts-de-France region, and the CPER “Photonics for Society” are acknowledged for financial support. This work was partly supported by the French Renatech network. A.V. thanks the i-SITE foundation of the University of Lille for a PhD fellowship. This project has received funding from the European Union’s Horizon 2020 Research and Innovation Staff Exchange (RISE) Marie Skłodowska-Curie Actions under grant agreement No 690836.

Published
2022
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32. Discovery of a Peptide Nucleic Acid (Pna) Aptamer for Cardiac Troponin I: Substituting DNA with Neutral Pna Maintains Picomolar Affinity and Improves Performances in Graphene Field-Effect Transistors (Gfet)

Author

Teresa Rodrigues, Federica Curti, Yann R. Leroux, Alexandre Barras, Quentin Pagneux, Henri Happy, Christoph Kleber, Rabah Boukherroub, Stefan Volpi, Maria Cereri, Roberto Corradini, Sabine Szunerits, and Wolfgang Knoll

Subjects
History, Polymers and Plastics, Business and International Management, Industrial and Manufacturing Engineering
Published
2022
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34. The Potential of Developing Pan-Coronaviral Antibodies to Spike Peptides in Convalescent COVID-19 Patients

Author

Roman Grytsko, Galyna Bila, Quentin Pagneux, Rabah Boukherroub, Sandor G. Vari, Alexandre Barras, Rostyslav Bilyy, Yuriy Rebets, Markian Samborskyy, Sabine Szunerits, Andrii Rabets, Danylo Halytsky Lviv National Medical University [Lviv, Ukraine], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Financial support from the Cedars-Sinai Medical Center’s International Research and Innovation in Medicine Program, the Association for Regional Cooperation in the Fieldsof Health, Science and Technology (RECOOP HST Association) RCSS grant 020, and BMYSRG 015, Grant of Ministry of Healthcare of Ukraine 0119U101338 and National Research Foundation of Ukraine 2020.02/0131, Volkswagen-Stiftung grant No 97744. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under Grant agreements No 861878 and 872331. Financial support by ANR project 'nanoMERS' (ANR- 8-CE09-0021) is acknowledged.NeutroCure and NoBiasFluors, ANR-18-CE09-0021,NanoMERS,Utilisation des nanoparticules innovantes pour inhiber les infections à coronavirus(2018), and European Project: 9872331(1998)

Subjects
Middle East respiratory syndrome coronavirus, Protein subunit, Short Communication, viruses, Immunology, COVID-9, Immunoglobulins, Peptide, Biology, Cross Reactions, medicine.disease_cause, Antibodies, Viral, Cross-reactivity, Antibodies, 03 medical and health sciences, [SPI]Engineering Sciences [physics], 0302 clinical medicine, medicine, Immunology and Allergy, Humans, Coronavirus, chemistry.chemical_classification, SARS-CoV-2, COVID-19, Convalescence, General Medicine, Virology, 3. Good health, Heptad repeat, chemistry, Severe acute respiratory syndrome-related coronavirus, Polyclonal antibodies, Spike Glycoprotein, Coronavirus, biology.protein, Middle East Respiratory Syndrome Coronavirus, Antibody, 030215 immunology
Abstract

Coronaviruses share conservative spike protein (S) on their enveloped membrane surface, where S1 subunit recognizes and binds the cellular receptor, and the S2 subunit mediates membrane fusion. This similarity raises the question: does coronaviral infection by one create protection to others? Convalescent SARS-CoV-2 (COVID-19) sera were tested for cross reactivity with peptides from Middle East respiratory syndrome coronavirus (MERS-CoV) which shares 74% homology. Our results showed significant cross-reactivity with a peptide of the heptad repeat 2 (HR2) domain of the MERS-CoV spike protein. Sera samples of 47 validated seropositive convalescent COVID-19 patients and 40 sera samples of control patients, collected in pre-COVID time were used to establish cross-bind reactivity with the MERS-CoV peptide. Significantly stronger binding (p

Published
2021
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35. Surface modification of carbon dots with tetraalkylammonium moieties for fine tuning their antibacterial activity

Author

Elizaveta Sviridova, Alexandre Barras, Ahmed Addad, Evgenii Plotnikov, Antonio Di Martino, Dominique Deresmes, Ksenia Nikiforova, Marina Trusova, Sabine Szunerits, Olga Guselnikova, Pavel Postnikov, Rabah Boukherroub, Tomsk Polytechnic University [Russie] (UPT), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme de Caractérisation Multi-Physiques - IEMN (PCMP - IEMN), University of Chemistry and Technology Prague (UCT Prague), This work was supported by the by RFBR, project number 20-33-90042. Financial support from the Centre National de la Recherche Scientifique (CNRS), the University of Lille, the Région Hauts-de-France, the Agence Nationale de la Recherche (MSN-2hv) and the CPER 'Photonics for Society' is acknowledged. ADM and MT acknowledge the Ministry of Science and Higher Education of the Russian Federation (State Project 'Science' №WSWW-2020-0011). This research work has been partially undertaken with the support of IEMN fabrication (CMNF) and characterization (PCMP) facilities., Renatech Network, CMNF, PCMP PCP, and ANR-19-CE09-0034,MSN-2hv,Matériaux théranostiques associant les fonctionnalités d'imagerie 2 photons et de thérapie photodynamique pour le ciblage de bactéries et le contrôle des infections lors de la cicatrisation(2019)

Subjects
Staphylococcus aureus, Tetraalkylammonium salts, Biomedical Engineering, Bioengineering, Staphylococcal Infections, Gram-Positive Bacteria, Carbon, Anti-Bacterial Agents, Biomaterials, [SPI]Engineering Sciences [physics], Mice, Biofilms, Diazonium chemistry, Gram-Negative Bacteria, Escherichia coli, Carbon dots, Animals, Antibacterial activity
Abstract

Materials for Biological Applications. Materials Science and Engineering: C has been renamed to Biomaterials Advances; International audience; The widespread of bacterial infections including biofilms drives the never-ending quest for new antimicrobial agents. Among the great variety of nanomaterials, carbon dots (CDs) are the most promising antibacterial material, but still require the adjustment of their surface properties for enhanced activity. In this contribution, we report a facile functionalization method of carbon dots (CDs) by tetraalkylammonium moieties using diazonium chemistry to improve their antibacterial activity against Gram-positive and Gram-negative bacteria. CDs were modified by novel diazonium salts bearing tetraalkylammonium moieties (TAA) with different alkyl chains (C2, C4, C9, C12) for the optimization of antibacterial activity. Variation of the alkyl chain allows to reach the significant antibacterial effect for CDs-C9 towards Gram-positive Staphylococcus aureus (S. aureus) (MIC = 3.09 ± 1.10 μg mL−1) and Gram-negative Escherichia coli (E. coli) (MIC = 7.93 ± 0.17 μg mL−1) bacteria. The antibacterial mechanism of CDs-C9 is ascribed to the balance between the positive charge and hydrophobicity of the alkyl chains. TAA moieties are responsible for enhanced adherence on the bacterial cell membrane, its penetration and disturbance of physiological metabolism. CDs-C9 were not effective in the generation of reactive oxygen species excluding the oxidative damage mechanism. In addition, CDs-C9 effectively promoted the antibiofilm treatment of S. aureus and E. coli biofilms outperforming previously-reported CDs in terms of treatment duration and minimal inhibitory concentration. The good biocompatibility of CDs-C9 was demonstrated on mouse fibroblast (NIH/3T3), HeLa and U-87 MG cell lines for concentrations up to 256 μg mL−1. Collectively, our work highlights the correlation between the surface chemistry of CDs and their antimicrobial performance.

Published
2021
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36. Water-Soluble Ruthenium (II) Complex Derived From Optically Pure Limonene and Its Microencapsulation Are Efficient Tools Against Bacterial Food Pathogen Biofilms: Escherichia coli, Staphylococcus aureus, Enteroccocus faecalis, and Listeria monocytogenes

Author

Nour-Eddine Chihib, Sakhr Alhuthali, Adem Gharsallaoui, Samah Mechmechani, Simon Khelissa, Alexandre Barras, Yousra El Fannassi, Mohamed Amin El Amrani, Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Université Abdelmalek Essaâdi (UAE), Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), This work was carried out within the framework of TOUBKAL PROJECT No. 43716SB. The authors thank Campus France and the Partenariats Hubert Curien program and Imperial Open Access Fund for their financial support. This program was carried out between Abdelmalek Essaadi University, Faculty of Sciences, Tetouan, Morocco and Lille University, Lille, France., Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), and Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)

Subjects
Microbiology (medical), antibacterial agent, complexation, chemistry.chemical_element, biofilm removal, medicine.disease_cause, Microbiology, Bacterial cell structure, Enterococcus faecalis, biofilm, [SPI]Engineering Sciences [physics], 03 medical and health sciences, Minimum inhibitory concentration, Listeria monocytogenes, [SDV.IDA]Life Sciences [q-bio]/Food engineering, medicine, [CHIM.COOR]Chemical Sciences/Coordination chemistry, 0502 Environmental Science and Management, 0503 Soil Sciences, Escherichia coli, 030304 developmental biology, Antibacterial agent, 0303 health sciences, ruthenium (II), biology, 030306 microbiology, Chemistry, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Combinatorial chemistry, QR1-502, Ruthenium, limonene, microencapsulation, food pathogen, Bacteria, 0605 Microbiology
Abstract

Bioactive aminooxime ligands based on optically pure (R)-limonene have been synthesized in two steps. Their ruthenium (II) cationic water-soluble complex was prepared by a reaction between dichloro (para-cymene) ruthenium (II) dimers and aminooxime ligands in a 1:2 molar ratio. Antibacterial and antibiofilm activities of the synthetized complex were assessed against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis. The results revealed that the ruthenium (II) complex has higher antibacterial and antibiofilm activities in comparison with free ligands or the enantiopure (R)-limonene. Moreover, microencapsulation of this complex reduced its cytotoxicity and improved their minimum inhibitory concentration and antibiofilm activity toward the considered bacteria. The ruthenium (II) complex targets the bacterial cell membrane, which leads to rapid leakage of intracellular potassium. Our study suggests that the developed ruthenium (II) complexes could be useful as an alternative to conventional disinfectants.

Published
2021
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37. Surface modification of carbon dots with tetraalkylammonium moieties for fine tuning their antibacterial activity

Author

Marina E. Trusova, Rabah Boukherroub, Olga Guselnikova, Evgenii Plotnikov, Pavel S. Postnikov, Elizaveta Sviridova, Ksenia Nikiforova, Alexandre Barras, D. Deresmes, Antonio Di Martino, and Sabine Szunerits

Subjects
chemistry.chemical_classification, Biocompatibility, Absorption spectroscopy, Chemistry, Biofilm, Surface modification, Fourier transform infrared spectroscopy, Antibacterial activity, Alkyl, Fluorescence spectroscopy, Nuclear chemistry
Abstract

In this contribution, we report a facile functionalization method of carbon dots (CDs) by tetraalkylammonium moieties using diazonium chemistry to improve their antibacterial activity against Gram-positive and Gram-negative bacteria. CDs were modified by novel diazonium salts bearing tetraalkylammonium moieties (TAA) with different alkyl chains (C2, C4, C9, C12) for the optimization of antibacterial activity. The functionalized CDs were characterized by X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared (FTIR) spectroscopy, ultraviolet–visible (UV-vis) absorption spectrophotometry and fluorescence spectroscopy to confirm the covalent binding of TAA onto the CDs’ surface. Variation of the alkyl chain allows to reach the strongest antibacterial effect for CDs-C9 towards Gram-positive S. aureus (MIC 3.09±1.10 µg mL-1) and Gram-negative E. coli (MIC 7.93±0.17 µg mL-1) bacteria. In addition, the antibiofilm properties of the CDs-C9 were assessed; full biofilm inhibition was achieved after 6 h (64 µg mL-1) and 2 h (128 µg mL-1) treatment for S. aureus biofilm and >60% suppression of biofilm mass after 6 h (128 µg mL-1) for E. coli biofilm. CDs-C9 demonstrated good biocompatibility on mouse fibroblast (NIH/3T3), HeLa and U-87 MG cell lines for concentrations up to 256 µg mL-1. Collectively, our work highlights the correlation between the surface chemistry of CDs and their antimicrobial performance.

Published
2021
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38. Colorimetric assay for the detection of dopamine using bismuth ferrite oxide (Bi

Author

Mehri, Razavi, Alexandre, Barras, Madjid, Ifires, Abir, Swaidan, Maryam, Khoshkam, Sabine, Szunerits, Mohsen, Kompany-Zareh, and Rabah, Boukherroub

Subjects
Peroxidases, Dopamine, Nanoparticles, Colorimetry, Serum Albumin, Bovine, Hydrogen Peroxide, Bismuth, Ferric Compounds
Abstract

This work describes the preparation of ternary bismuth ferrite oxide nanoparticles (Bi

Published
2021

39. Adsorption-reduction of Cr(VI) onto unmodified and phytic acid-modified carob waste: Kinetic and isotherm modeling

Author

Kenza Bouaouina, Alexandre Barras, Nacer Bezzi, Mohammed A. Amin, Sabine Szunerits, Rabah Boukherroub, Laboratoire de Technologie des Matériaux et Génie des Procédés, Université Abderrahmane Mira [Béjaïa], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Taif University (TU), Partenaires INRAE, and The authors gratefully acknowledge financial support of the Algerian ministry of high education scientific research for 7 months PROFAS B+ scholarship, the Centre National de la Recherche Scientifique (CNRS), the University of Lille and the Hauts-de-France region. The authors also thank the Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, Saudi Arabia.

Subjects
Chromium, Carob waste, Environmental Engineering, Phytic Acid, Health, Toxicology and Mutagenesis, Public Health, Environmental and Occupational Health, General Medicine, General Chemistry, Kinetic-isotherm, Hydrogen-Ion Concentration, Pollution, Galactans, Mannans, [SPI]Engineering Sciences [physics], Kinetics, Cr(VI) adsorption, Plant Gums, Spectroscopy, Fourier Transform Infrared, Environmental Chemistry, Adsorption, Thermodynamic modeling, Water Pollutants, Chemical
Abstract

International audience; Carob waste (CW) is an agro-biomass material abundant in nature with potential use for eco-friendly remediation. However, like many biomass-based adsorbents, it suffers from its low adsorption capacity for organic/inorganic pollutants. Therefore, modification using physical and/or chemical means is commonly applied to improve the adsorptive properties of biomass-based adsorbents. In this study, carob waste (CW) and carob waste functionalized with phytic acid (PA-CW), as an ecofriendly product, were applied for the first time for Cr(VI) elimination. Various methods were applied for the material characterization like Fourier-transform infrared spectroscopy, powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA and DTG), X-ray photoelectron spectroscopy (XPS), specific surface area and porosity measurements. The results proved that both CW and PA-CW own appropriate features for efficient adsorption. Bach experiments revealed that the optimum parameters for Cr(VI) (100 mg/mL) removal at 25 °C were pH 2, 0.05 and 0.025 g as adsorbent dose for CW and PA-CW, respectively, over 120 min contact time. The kinetic of adsorption was well-described by the pseudo-second order model, whereas the isotherm modeling fitted well the modified Langmuir model. CW and PA-CW achieved respectively maximum adsorption capacities of 212.4 and 387.9 mg/g, which are among the highest values so far reported for biomass-based adsorbent materials. These results confirmed that CW and PA-CW could be alternative cost-effective adsorbents even for high concentrations of Cr(VI) in industrial wastewaters along with their reduction capacity.

Published
2021
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40. Flower‐like nitrogen‐Co‐doped MoS2@rgo composites with excellent stability for supercapacitors

Author

Min Li, Ahmed Addad, Rabah Boukherroub, Pascal Roussel, Yuan Zhang, Mohammed A. Amin, Sabine Szunerits, Alexandre Barras, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette] (LSCE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), The authors thank the Centre National de la Recherche Scientifique (CNRS), the University of Lille, and the Hauts-de-France region for financial support. The authors acknowledge financial support from the Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, SaudiArabia., Institut de Chimie du CNRS (INC)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), and Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille

Subjects
Ammonium molybdate, Supercapacitor, Materials science, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 7. Clean energy, 01 natural sciences, Nitrogen, Capacitance, Catalysis, Energy storage, Hydrothermal circulation, 0104 chemical sciences, chemistry.chemical_compound, [SPI]Engineering Sciences [physics], chemistry, Electrochemistry, Composite material, 0210 nano-technology, Molybdenum disulfide, Co doped
Abstract

International audience; Nitrogen-co-doped MoS2@rGO composites (named hereafter N-MoS2@N-RGO) are prepared by the hydrothermal method using dicyandiamide or hydrazine monohydrate as the nitrogen source. N-MoS2@rGO is obtained by the hydrothermal reaction of thioacetamide, ammonium molybdate and GO precursors in the presence of dicyandiamide, while N-MoS2@N-RGO is synthesized using the same precursors in the presence of hydrazine monohydrate as the nitrogen source. On the one hand, N-MoS2@RGO displays excellent performance and good capacitance value of 335 F/g acquired at 0.5A/g and improved stability (∼94% of the original capacitance is preserved after 5000 cycles). On the other hand, compared with N-MoS2@RGO, N-MoS2@N-RGO possesses a slightly higher capacitance of 340 F/g recorded at 0.5A/g and better cycling stability (∼98% of its original value after 5000 cycles). Taken together, the results of this investigation clearly indicate that the fabricated composites have application prospect in energy storage devices.

Published
2021
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41. CoO Promoted the Catalytic Activity of Nitrogen-Doped MoS2 Supported on Carbon Fibers for Overall Water Splitting

Author

Mohammed A. Amin, Alexandre Barras, Yuan Zhang, Sabine Szunerits, Ahmed Addad, Rabah Boukherroub, Liuqing Pang, Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, York-Zhejiang Lab. for Cognitive Radio & Green Commun., Zhejiang University, Unité Matériaux et Transformations - UMR 8207 (UMET), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Institut National de la Recherche Agronomique (INRA), Institut de Chimie du CNRS (INC)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Kinetics, Nitrogen doped, [CHIM.MATE]Chemical Sciences/Material chemistry, 02 engineering and technology, Electrolyte, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Catalysis, Metal, Chemical engineering, visual_art, visual_art.visual_art_medium, [CHIM]Chemical Sciences, Water splitting, General Materials Science, Hydrogen evolution, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract

Non-noble metal electrocatalysts have recently witnessed increasing attention for the hydrogen evolution reaction (HER) in acidic electrolytes. However, in alkaline electrolytes, the slow kinetics ...

Published
2019
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42. Rapid generation of coronaviral immunity using recombinant peptide modified nanodiamonds

Author

Sandrine Belouzard, Roman Grytsko, Sabine Szunerits, Rabah Boukherroub, Alexandre Barras, Rostyslav Bilyy, Galyna Bila, Jean Dubuisson, Yuri Lebedin, Nathan François, Karin Séron, Quentin Pagneux, Danylo Halytsky Lviv National Medical University [Lviv, Ukraine], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), XEMA-MEDICA Co, NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), This research was funded by the Centre National de la Recherche Scientifique (CNRS), the University of Lille, the ANR, the CPER 'Photonics for Society' and the Agence Nationale de la Recherche (ANR-nanoMERS). Financial support from the Grant of Ministry of Healthcare of Ukraine 0119U101338 and National Research Foundation of Ukraine 2020.02/0131, Volkswagen-Stiftung grant No 97744. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreements No 861878., ANR-18-CE09-0021,NanoMERS,Utilisation des nanoparticules innovantes pour inhiber les infections à coronavirus(2018), European Project: 8618780(1987), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), IEMN, Collection, APPEL À PROJETS GÉNÉRIQUE 2018 - Utilisation des nanoparticules innovantes pour inhiber les infections à coronavirus - - NanoMERS2018 - ANR-18-CE09-0021 - AAPG2018 - VALID, and Biopolymer Sequence Analysis: New Methods and Instrumentation - 1987-09-01 - 1991-04-30 - 8618780 - VALID

Subjects
Microbiology (medical), coronavirus peptide, diamond nanoparticles, neutrophil extracellular traps, [SPI] Engineering Sciences [physics], medicine.medical_treatment, Antigen presentation, 02 engineering and technology, Biology, Article, 03 medical and health sciences, [SPI]Engineering Sciences [physics], Immune system, Antigen, Immunity, medicine, Immunology and Allergy, Molecular Biology, 030304 developmental biology, 0303 health sciences, General Immunology and Microbiology, 021001 nanoscience & nanotechnology, Virology, 3. Good health, Vaccination, Infectious Diseases, Immunization, biology.protein, Medicine, Antibody, 0210 nano-technology, Adjuvant
Abstract

International audience; Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that the best possible antigenic components are chosen to stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based adjuvants are proposed here as a rapid and versatile platform for antigen conjugation, utilizing peptides common to different pathogenic strains and making this strategy a good candidate for a “ready-to-use” vaccine. Initiation of an inflammatory reaction with a resulting immune response is based on the ability of living organisms to entrap nanostructures such as nanodiamonds with neutrophil extracellular traps (NETs) formation. In this work, coronavirus peptide homological for MERS-CoV, fusion inhibitor, was conjugated to nanodiamonds and used to induce neutrophilic-driven self-limiting inflammation. The resulting adjuvant was safe and did not induce any tissue damage at the site of injection. Mice immunization resulted in IgG titers of ¼,000 within 28 days. Immunization of rabbits resulted in the formation of a high level of antibodies persistently present for up to 120 days after the first immunization (animal lifespan ~3 years). The peptide used for immunization proved to be reactive with sera of convalescent COVID patients, demonstrating the possibility of developing pancoronaviral vaccine candidates.

Published
2021
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43. Enhanced electrocatalytic activity of PtRu/nitrogen and sulphur co-doped crumbled graphene in acid and alkaline media

Author

Alexandre Barras, Yuanyuan Miao, Pascal Roussel, Siddheshwar N. Bhange, Sabine Szunerits, Sreekumar Kurungot, Ahmed Addad, Rabah Boukherroub, Liuqing Pang, Mohammed A. Amin, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille, CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
ORR, chemistry.chemical_element, Nanoparticle, 02 engineering and technology, Overpotential, 010402 general chemistry, Electrocatalyst, 7. Clean energy, 01 natural sciences, law.invention, Catalysis, Biomaterials, [SPI]Engineering Sciences [physics], Colloid and Surface Chemistry, law, Sulphur and nitrogen co-doped crumbled graphene, Graphene, graphene, Oxygen evolution, HER, PtRu2, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Nitrogen, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, chemistry, OER, 0210 nano-technology, Platinum, Nuclear chemistry
Abstract

International audience; The low mass activity and high price of pure platinum (Pt)-based catalysts predominantly limit their large-scale utilization in electrocatalysis. Therefore, the reduction of Pt amount while preserving the electrocatalytic efficiency represents a viable alternative. In this work, we prepared new PtRu2 nanoparticles supported on sulphur and nitrogen co-doped crumbled graphene with trace amounts of iron (PtRu2/PF) electrocatalysts. The PtRu2/PF catalysts exhibited enhanced electrocatalytic performance and stability for the hydrogen evolution reaction (HER) at pH - 0. Moreover, the prepared PtRu2/PF electrocatalyst displayed higher HER activity than commercial 20% Pt/C. The PtRu2/PF catalyst achieved a current density of 10 mA cm-2 at an overpotential value of only 22 mV for HER, performing better activity than many other Pt-based electrocatalysts. Besides, the PtRu2/PF revealed a good performance for the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) in alkaline media. The PtRu2/PF catalyst recorded a current density of 10 mA cm-2 at an overpotential of only 270 mV for OER in KOH (1.0 M) solution and an onset potential of 0.96 V vs. RHE (at 1 mA cm-2) for ORR in KOH (0.1 M) solution.

Published
2021
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44. Colorimetric detection of chromium (VI) ion using poly(N-phenylglycine) nanoparticles acting as a peroxidase mimetic catalyst

Author

Farnoush Faridbod, Alexandre Barras, Sabine Szunerits, Abir Swaidan, Rabah Boukherroub, Sena Ghayyem, Mathias Dolci, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), and Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)

Subjects
Chromium, Glycine, Nanoparticle, chemistry.chemical_element, 02 engineering and technology, Poly(N-phenylglycine) nanoparticles, 01 natural sciences, Analytical Chemistry, Catalysis, Absorbance, chemistry.chemical_compound, [SPI]Engineering Sciences [physics], Hexavalent chromium, Hydrogen peroxide, Chromium(VI) ion, Peroxidase, Detection limit, 010401 analytical chemistry, Substrate (chemistry), Hydrogen Peroxide, 021001 nanoscience & nanotechnology, Peroxidase-like activity, 0104 chemical sciences, Peroxidases, chemistry, Heavy metals ion, Nanoparticles, Colorimetry, 0210 nano-technology, Colorimetric detection, Nuclear chemistry
Abstract

International audience; This paper reports on enzyme-like catalytic properties of polyethylene glycol-functionalized poly(N-phenylglycine) (PNPG-PEG) nanoparticles, which have not been explored to date. The developed nanoparticles have the ability to display great inherent peroxidase-like activity at very low concentrations, and are able to catalyze the oxidation of 3,3',5,5'tetramethylbenzidine (TMB) substrate in presence of hydrogen peroxide (H2O2). The oxidized product of TMB has a deep blue color with a maximum absorbance at ~655 nm. The PNPG-PEG nanoparticles exhibit Km values of 0.2828 for TMB and 0.0799 for H2O2, indicating that TMB oxidation takes place at lower concentration of H2O2 in comparison to other nanozymes. Based on the known mechanism of H2O2 oxidation by hexavalent chromium [Cr(VI)] ions to generate hydroxyl radicals (.OH), these nanoparticles were successfully applied for the colorimetric sensing of Cr(VI) ions. The sensor achieved good performance for Cr(VI) sensing with detection limits of 0.012 μM (0.01-0.1 μM linear range) and 0.52 μM (0.05-12.5 μM linear range). The detection scheme was highly selective, and successfully applied for the detection of Cr(VI) in real water samples.

Published
2021
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45. Cathodic pre-polarization studies on the carbon felt/KOH interface: An efficient metal-free electrocatalyst for hydrogen generation

Author

Rabah Boukherroub, Alexandre Barras, Toufik Hadjersi, Sabine Szunerits, Redouane Chegroune, Madjid Ifires, Ahmed Addad, Mohammed A. Amin, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université de Lille, UDL, Centre National de la Recherche Scientifique, CNRS, and The authors gratefully acknowledge financial support from the Centre National de la Recherche Scientifique (CNRS), the University of Lille, and the Hauts-de-France region. The authors are also thankful to the Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, Saudi Arabia.

Subjects
Materials science, General Chemical Engineering, Inorganic chemistry, 02 engineering and technology, engineering.material, Overpotential, 010402 general chemistry, Electrocatalyst, Electrochemistry, 01 natural sciences, Cathodic protection, [SPI]Engineering Sciences [physics], Polarization (electrochemistry), Carbon felt, Tafel equation, Alkaline electrolytes, Chronoamperometry, 021001 nanoscience & nanotechnology, Hydrogen evolution reaction, 0104 chemical sciences, engineering, Noble metal, 0210 nano-technology, Cathodic activation, Electrocatalysis
Abstract

International audience; The recent decades have witnessed a booming research effort on the development of highly active electrode materials in electrocatalysis with the aim to replace the commonly used noble metal-based ones. Although non-noble metal electrodes can fulfill some requirements in terms of performance and price, their stability in alkaline media is generally weak. Additionally, from the environmental view point, the use of metals can cause water contamination. Therefore, the preparation of metal-free electrocatalysts represents an appealing approach to overcome the above-mentioned shortcuts. In this work, we used a simple electrochemical activation process, chronoamperometry measurements conducted in 1 M KOH at various overpotentials (−2, −2.5, −3, and −4 V vs. RHE) and pre-polarization periods (0.5, 2, 4, and 12 h), of commercially available and cheap carbon felt (CF) electrode to achieve good performance for hydrogen evolution reaction (HER) in alkaline media. Under optimized operating conditions (12 h of chronoamperometry measurement acquired at an overpotential of −4 V vs. RHE), the CF electrode exhibited an overpotential of 182 mV at 10 mA/cm2, a value surpassing that recorded for the most efficacious metal-free HER electrocatalysts published in the literature. The two CF samples exposed to 12 h of cathodic activation at −2.5 and −3.0 V vs. RHE recorded respectively Tafel slope (bc) values of 110 and 126 mV dec−1. These bc values are close to the standard bc value of 120 mV dec−1, suggesting the Volmer step as the main step controlling the HER. Increasing the applied cathodic potential to −4.0 V vs. RHE further decreased the bc value to 90 mV dec−1, referring to increased accessible active catalytic sites for the HER. The cathodically activated CF surface's morphology and composition were assessed by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) examinations. Raman spectroscopy was also conducted to assess the influence of the cathodic activation process on the structural defects/disorder of the CF surface. The formation of oxygen-containing groups on the CF surface after cathodic activation, as evidenced from XPS analysis, is believed to enhance water adsorption and act as active sites for hydrogen generation. The technique developed herein is simple and straightforward and can be easily implemented for almost any type of electrode.

Published
2021
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46. Simultaneous photocatalytic Cr(VI) reduction and phenol degradation over copper sulphide-reduced graphene oxide nanocomposite under visible light irradiation: Performance and reaction mechanism

Author

Ahcène Chaouchi, Ahmed Addad, Baghdad Ouddane, Alexandre Barras, Yacine Cherifi, Rabah Boukherroub, Pascal Roussel, Sabine Szunerits, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille, Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), and Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Chromium, Environmental Engineering, Materials science, Light, rGO/CuS nanocomposites, Health, Toxicology and Mutagenesis, 0208 environmental biotechnology, Oxide, 02 engineering and technology, 010501 environmental sciences, Sulfides, 01 natural sciences, Nanocomposites, chemistry.chemical_compound, symbols.namesake, [SPI]Engineering Sciences [physics], X-ray photoelectron spectroscopy, Spectrophotometry, medicine, Phenol degradation, Environmental Chemistry, Phenol, Photocatalysis, Spectroscopy, 0105 earth and related environmental sciences, Nanocomposite, medicine.diagnostic_test, Public Health, Environmental and Occupational Health, General Medicine, General Chemistry, [CHIM.CATA]Chemical Sciences/Catalysis, Pollution, 6. Clean water, 020801 environmental engineering, chemistry, 13. Climate action, symbols, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Graphite, Raman spectroscopy, Copper, Nuclear chemistry, Cr(VI) removal
Abstract

International audience; The contamination of water by synthetic organic molecules and trace metals is a growing challenge, in spite of the enormous research efforts being made in the field of water treatment. In this study, reduced graphene oxide-copper sulphide (rGO-CuS) nanocomposites of different rGO/CuS (2/1, 1/1, 1/2) molar ratios were fabricated via a facile one-step hydrothermal method. The nanocomposite materials, named hereafter as 2rGO-CuS, rGO-CuS and rGO-2CuS, were characterized using various analytical techniques, including X-ray diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectroscopy, X-ray photoelectron spectroscopy (XPS) and UV-visible spectrophotometry. The photocatalytic performance of the nanocomposites was assessed under visible light irradiation (λ > 420 nm) for the simultaneous photocatalytic reduction of Cr(VI) and phenol degradation. It was found that rGO-2CuS achieved a remarkable enhancement of the photocatalytic activity among the prepared nanocomposites for the degradation of phenol and reduction of Cr(VI). Therefore, the simultaneous photocatalytic phenol degradation and Cr(VI) reduction over rGO-2CuS sample was further investigated. The experimental results revealed that rGO-2CuS catalyst maintained good degradation efficacy of mixed pollutants after 6 runs and dissolved oxygen was found to be essential to promote Cr(VI) reduction and phenol degradation. A detailed photocatalytic activity under visible light irradiation mechanism was proposed based on quenching experiments and fluorescence measurements.

Published
2021
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47. Aryne cycloaddition reaction as a facile and mild modification method for design of electrode materials for high-performance symmetric supercapacitor

Author

Rabah Boukherroub, Pavel S. Postnikov, Mekhman S. Yusubov, Elizaveta Sviridova, Akira Yoshimura, Alexandre Barras, Min Li, Ahmed Addad, Viktor V. Zhdankin, Sabine Szunerits, Tomsk Polytechnic University [Russie] (UPT), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), and Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Thermogravimetric analysis, Materials science, General Chemical Engineering, 02 engineering and technology, 010402 general chemistry, 7. Clean energy, 01 natural sciences, law.invention, [SPI]Engineering Sciences [physics], X-ray photoelectron spectroscopy, law, Aryne cycloaddition reaction, Electrochemistry, [CHIM]Chemical Sciences, Reduced graphene oxide, Fourier transform infrared spectroscopy, Supercapacitor, Graphene, Covalent functionalization, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Dielectric spectroscopy, Chemical engineering, Iodonium salts, Surface modification, Cyclic voltammetry, 0210 nano-technology, Symmetric supercapacitor
Abstract

International audience; Covalent modification of graphene-based materials can be considered as one of the most promising methods for tailoring their electrochemical properties and extending their application as electrode materials for supercapacitors. In this contribution, we report a facile and mild approach for the covalent functionalization of reduced graphene oxide (rGO) via aryne cycloaddition using pseudocyclic iodoxoborole as an aryne source. The structure and chemical composition of the functionalized rGO (f-rGO) were assessed by Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), ultraviolet-visible (UV-vis) spectrophotometry, Raman spectroscopy and X-ray photoelectron spectroscopy (XPS), which revealed the negligible influence of covalent modification on the rGO structure. Transmission electron microscopy (TEM) imaging showed an increase of the interlayer distance from 0.38 to 0.46 nm upon functionalization. The electrochemical performance of f-rGO material was studied by cyclic voltammetry (CV), galvanostatic charge-discharge (GCD) and electrochemical impedance spectroscopy (EIS) techniques in 2 M KOH aqueous solution as the electrolyte. Under optimized conditions, the f-rGO displayed a high specific capacitance of 297 F g-1 at a current density of 1 A g-1, which is much higher than that of unmodified rGO (170 F g-1 at 1 A g-1). Therefore, the f-rGO was used to construct a symmetric supercapacitor device, exhibiting an energy density of 6.7 Wh kg-1 at a power density of 685.8 W kg-1. The device exhibited good cycling stability and ability to maintain about 96% of the initial capacitance value after 10,000 cycles. Furthermore, two symmetric supercapacitor devices were successfully applied to power a home-designed windmill device for 3 s. The results obtained in the present study highlight the importance of graphene functionalization as an effective route to fabricate rGO-based materials with enhanced properties in energy storage devices.

Published
2021
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48. The impact of chemical engineering and technological advances on managing diabetes: present and future concepts: [Review]

Author

Rabah Boukherroub, Sabine Szunerits, Amar Abderrahmani, Sorin Melinte, Alexandre Barras, Anna Voronova, Quentin Pagneux, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), UCL - SST/ICTM/ELEN - Pôle en ingénierie électrique, Université catholique de Lille (UCL)-Université catholique de Lille (UCL), and Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)

Subjects
Blood Glucose, medicine.medical_treatment, Insulin delivery, Context (language use), Biosensing Techniques, 02 engineering and technology, 03 medical and health sciences, Drug Delivery Systems, Insulin-Secreting Cells, Diabetes mellitus, Diabetes Mellitus, medicine, Animals, Humans, Hypoglycemic Agents, Insulin, 030304 developmental biology, 0303 health sciences, Genetic Therapy, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, medicine.disease, 3. Good health, Drug development, Chemical engineering, Informatics, Drug delivery, 0210 nano-technology, Blood stream
Abstract

International audience; Monitoring blood glucose levels for diabetic patients is critical to achieve tight glycaemic control. As none of the current antidiabetic treatments restore lost functional β-cell mass in diabetic patients, insulin injections and the use of insulin pumps are most widely used in the management of glycaemia. The use of advanced and intelligent chemical engineering, together with the incorporation of micro- and nanotechnological-based processes have lately revolutionized diabetic management. The start of this concept goes back to 1974 with the description of an electrode that repeatedly measures the level of blood glucose and triggers insulin release from an infusion pump to enter the blood stream from a small reservoir upon need. Next to the insulin pumps, other drug delivery routes, including nasal, transdermal and buccal, are currently investigated. These processes necessitate competences from chemists, engineers-alike and innovative views of pharmacologists and diabetologists. Engineered micro and nanostructures hold a unique potential when it comes to drug delivery applications required for the treatment of diabetic patients. As the technical aspects of chemistry, biology and informatics on medicine are expanding fast, time has come to step back and to evaluate the impact of technology-driven chemistry on diabetics and how the bridges from research laboratories to market products are established. In this review, the large variety of therapeutic approaches proposed in the last five years for diabetic patients are discussed in an applied context. A survey of the state of the art of closed-loop insulin delivery strategies in response to blood glucose level fluctuation is provided together with insights into the emerging key technologies for diagnosis and drug development. Chemical engineering strategies centered on preserving and regenerating functional pancreatic β-cell mass are evoked in addition as they represent a permanent solution for diabetic patients.

Published
2021
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49. Anti-biofilm activity of dodecyltrimethylammonium chloride microcapsules against

Author

Simon, Khelissa, Adem, Gharsallaoui, Jian, Wang, Emilie, Dumas, Alexandre, Barras, Charafeddine, Jama, Fouzia, Jbilou, Noureddine, Loukili, and Nour-Eddine, Chihib

Subjects
Quaternary Ammonium Compounds, Staphylococcus aureus, Salmonella enteritidis, Biofilms, Capsules
Abstract

Dodecyltrimethylammonium chloride (DTAC) was trapped into maltodextrins/pectin spray dried microcapsules to improve its activity against

Published
2021

50. Anti-biofilm activity of dodecyltrimethylammonium chloride microcapsules against Salmonella enterica serovar Enteritidis and Staphylococcus aureus

Author

Nour-Eddine Chihib, Alexandre Barras, Noureddine Loukili, Adem Gharsallaoui, Emilie Dumas, Jian Wang, Simon Khelissa, Fouzia Jbilou, charafeddine Jama, Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université de Lille, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), This work was carried out within the framework of ALIBIOTECH program (Agroalimentaire et Biotechnologie). The authors also thank the Haut de France region and FEDER (Fonds Européen de Développement Régional) for their financial support., Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), and Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)

Subjects
0301 basic medicine, Staphylococcus aureus, food.ingredient, Pectin, Salmonella enteritidis, 030106 microbiology, Aquatic Science, medicine.disease_cause, Applied Microbiology and Biotechnology, 03 medical and health sciences, food, Dodecyltrimethylammonium chloride, medicine, Food science, spray drying, Cytotoxicity, Water Science and Technology, Chemistry, Biofilm, food and beverages, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, 030104 developmental biology, anti-biofilm activity, Spray drying, microencapsulation, Intracellular
Abstract

International audience; Dodecyltrimethylammonium chloride (DTAC) was trapped into maltodextrins/pectin spray dried microcapsules to improve its activity against Salmonella enteritidis and Staphylococcus aureus biofilms. Two different microcapsules were prepared: uncomplexed DTAC-microcapsules (UDM), containing DTAC and maltodextrins; and complexed DTAC-microcapsules (CDM) containing DTAC complexed with pectin and maltodextrins. The minimum inhibitory concentrations (MIC) of both free and microencapsulated DTAC were investigated against S. Enteritidis and S. aureus. The MICs of DTAC were significantly lower when encapsulated. CDM treatment resulted in a 2 and 3.2 log reduction in S. aureus and S. Enteritidis biofilm culturable biomass, respectively. Microencapsulation reduced the cytotoxicity of DTAC by up to 32-fold. Free DTAC and CDM targeted the cell membrane resulting in the leakage of the intracellular molecules and subsequent cell death. The development of DTAC microcapsules reduced the amount of DTAC required to maintain the high standards of cleanliness and hygiene required in the food processing industries.

Published
2021
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