1. Brown and White Adipose Tissue Expression of IL6, UCP1 and SIRT1 are Associated with Alterations in Clinical, Metabolic and Anthropometric Parameters in Obese Humans
- Author
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Alanna Fernandes Paraíso, Sérgio Henrique Sousa Santos, Gislaine Candida Batista Jorge, Lara Jhullian Tolentino Vieira, João Marcus Oliveira Andrade, Antonio Sergio Barcala Jorge, André Luiz Sena Guimarães, Aline Mourão Hilzenderger, Raíssa M. Porto Franco, and Alfredo Maurício Batista De-Paula
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Mrna expression ,Adipose Tissue, White ,Adipose tissue ,030209 endocrinology & metabolism ,White adipose tissue ,Biology ,Anthropometric parameters ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Adipose Tissue, Brown ,Sirtuin 1 ,Internal medicine ,Gene expression ,Internal Medicine ,medicine ,Humans ,Obesity ,Uncoupling Protein 1 ,Interleukin-6 ,food and beverages ,General Medicine ,Anthropometry ,Middle Aged ,medicine.disease ,030104 developmental biology ,Gene Expression Regulation ,Female ,hormones, hormone substitutes, and hormone antagonists - Abstract
Aim: The present study aimed to analyze the expression of IL6, UCP1 and SIRT1 in adipose tissue (WAT and BAT) in association to clinical, metabolic and anthropometric parameters in obese humans. Methods: WAT and BAT samples from obese patients (n=27) were collected. IL6, UCP1 and SIRT1 markers were measured by qRT-PCR. The association between IL6, UCP1 and SIRT1 mRNA expression and anthropometric and clinical parameters were evaluated, using appropriate statistical tests. Results: Our results demonstrated that high levels of IL6 are associated with altered glucose levels in the WAT (p=0.01). In contrast, high levels of IL6 in the BAT were associated with decreased % fat (p=0.01) and fat weight (p=0.02) and increased mVO2 (p=0.02) and VO2 (p=0.02). For UCP1, a higher expression in the BAT was observed when compared to the WAT (p=0.0001). This gene expression was associated with lower values of BMI (p=0.03), % fat (P=0.02) and fat weight (P=0.02) and increased mVO2 (p=0.041) and VO2 (p=0.001). In the WAT, decreased levels of SIRT1 were associated with increased fat weight (p=0.02); in the BAT, associations were found for % fat (p=0.018) and mVO2 (p=0.03). Conclusion: These results reveal different characteristics in the biological actions between WAT and BAT in obese humans. Increased levels of IL6, UCP1 and SIRT1 in the BAT were associated with metabolic parameters improvements.
- Published
- 2017